Rmu_sc0000293.1_g000037

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000293.1
OG0010011
Physical Location & Seq
Forward (+)
180324 .. 184427
4104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000293.1_g000037.1.cds

Sequence Viewer

Length: 1092 bp
atggcaacgtgggctgcagtgggttgcagcggcacaggtaggcaggcagggcgcgggccggcttgcagcagcttctgcaacagggcagtagcgcaggtgtgcgggctggtgcggagtttgcagggggcagcaggttcggttgcagctagttcggcaggaggctgtagctggttaggcaggaggctgcaggggcaaggctacggtgctgggcagcaggtaggttggcgggctgctgcggtagtgggctgcaggcttgaggcagggaggctgcaggctggtgcaaggacgtgtgcgggatggtgcgcaggttgcagctgtgcggcaggaggctgggctgcagctaggatggagagaattaggaagcagagccgaggtggcgatgaattggccttggtaaaggcggccgcatgggcatggtatgagcatgggactggattagaaggcaagtcactgcaggaatttgatatcagaagaacttttcatgatcctataccatccaggttcaagctagaagccaagaaattagccgaacaaggcagtagagtttttgatgaagggtcaagtccgggaactagtgggagtgtacagactgttgatgatcattcacttcttgatagatatgaaattgagagtatttctaggcggttggatcaacttatagagtctagtggtgacaaaggcacagtttataataaggggttcctagttggagatcgtcttgctaaactgtacgaccactctaatcttggatgcggtagtagatgctcaccaccgccgaagttacaagttccgacgtctgcagttgaagatcttcatcgaaaaggaagtgtggcattatcagatggtgactacacaaatcgcaggaggaagaaaacgagcaacaactttagagggttttggcaaaggcatgtagtgatatgcggttcaagtactcataaagatgtaattctagggacaagatcttttgtggataatcgggggcagcagcagaacccggaaaagcggaaagcatactcgccagtaccagtggtaaagttggccaactgtcggccgagggccagtggccgtcacagacgccttgtgatcaattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

363

Amino Acids

39.05

Weight (kDa)

9.88

Isoelectric Point (pI)

46.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 690
AarI CACCTGC 1 cut(s) 85
AatII GACGTC 1 cut(s) 797
Acc16I TGCGCA 1 cut(s) 304
Acc36I ACCTGC 4 cut(s) 85, 122, 205, 296
AccII CGCG 1 cut(s) 54
AclWI GGATC 2 cut(s) 479, 657
AcoI YGGCCR 4 cut(s) 402, 1038, 1049, 1063
AcsI RAATTY 1 cut(s) 458
AcyI GRCGYC 2 cut(s) 794, 1075
AfaI GTAC 4 cut(s) 585, 731, 931, 1023
AfiI CCNNNNNNNGG 1 cut(s) 1047
AflIII ACRYGT 1 cut(s) 287
AgsI TTSAA 3 cut(s) 505, 806, 927
AhlI ACTAGT 1 cut(s) 572
AjiI CACGTC 1 cut(s) 288
AjnI CCWGG 1 cut(s) 497
AluBI AGCT 6 cut(s) 72, 146, 168, 315, 341, 508
AluI AGCT 6 cut(s) 72, 146, 168, 315, 341, 508
AlwI GGATC 2 cut(s) 479, 657
AlwNI CAGNNNCTG 1 cut(s) 75
AoxI GGCC 7 cut(s) 56, 387, 402, 1038, 1049, 1056, 1063
ApoI RAATTY 1 cut(s) 458
Asp700I GAANNNNTTC 1 cut(s) 810
AspLEI GCGC 3 cut(s) 54, 94, 305
AspS9I GGNCC 2 cut(s) 56, 1056
AsuC2I CCSGG 2 cut(s) 567, 995
AsuHPI GGTGA 3 cut(s) 683, 759, 857
BalI TGGCCA 1 cut(s) 1040
BccI CCATC 4 cut(s) 291, 340, 502, 836
BceAI ACGGC 1 cut(s) 1050
BcgI CGANNNNNNTGC 4 cut(s) 117, 132, 151, 166
BciT130I CCWGG 1 cut(s) 499
BclI TGATCA 2 cut(s) 598, 1083
BcnI CCSGG 2 cut(s) 567, 995
BcuI ACTAGT 1 cut(s) 572
BfaI CTAG 8 cut(s) 147, 342, 509, 573, 639, 666, 704, 950
BfmI CTRYAG 8 cut(s) 15, 163, 185, 247, 269, 336, 452, 798
BfuAI ACCTGC 4 cut(s) 85, 122, 205, 296
BglI GCCNNNNNGGC 2 cut(s) 375, 410
BglII AGATCT 2 cut(s) 808, 959
BmcAI AGTACT 1 cut(s) 931
Bme1390I CCNGG 3 cut(s) 499, 567, 995
BmgBI CACGTC 1 cut(s) 288
BmgT120I GGNCC 2 cut(s) 56, 1056
BmiI GGNNCC 1 cut(s) 701
BmrFI CCNGG 3 cut(s) 499, 567, 995
BmsI GCATC 2 cut(s) 740, 752
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 2 cut(s) 567, 995
BsaBI GATNNNNATC 1 cut(s) 813
BsaHI GRCGYC 2 cut(s) 794, 1075
BsaJI CCNNGG 3 cut(s) 370, 390, 1052
Bsc4I CCNNNNNNNGG 1 cut(s) 1047
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 4 cut(s) 436, 1019, 1025, 1059
Bse8I GATNNNNATC 1 cut(s) 813
BseBI CCWGG 1 cut(s) 499
BseDI CCNNGG 3 cut(s) 370, 390, 1052
BseGI GGATG 4 cut(s) 302, 351, 494, 755
BseJI GATNNNNATC 1 cut(s) 813
BseLI CCNNNNNNNGG 1 cut(s) 1047
BseNI ACTGG 4 cut(s) 436, 1019, 1025, 1059
BseX3I CGGCCG 2 cut(s) 402, 1049
BseYI CCCAGC 2 cut(s) 206, 330
Bsh1236I CGCG 1 cut(s) 54
Bsh1285I CGRYCG 2 cut(s) 405, 1052
BshFI GGCC 7 cut(s) 58, 389, 404, 1040, 1051, 1058, 1065
BsiEI CGRYCG 2 cut(s) 405, 1052
BsiSI CCGG 3 cut(s) 59, 566, 995
BslFI GGGAC 2 cut(s) 442, 967
BslI CCNNNNNNNGG 1 cut(s) 1047
BsmFI GGGAC 2 cut(s) 442, 967
BsnI GGCC 7 cut(s) 58, 389, 404, 1040, 1051, 1058, 1065
Bsp1407I TGTACA 1 cut(s) 583
Bsp143I GATC 7 cut(s) 484, 598, 649, 712, 808, 959, 1083
BspANI GGCC 7 cut(s) 58, 389, 404, 1040, 1051, 1058, 1065
BspFNI CGCG 1 cut(s) 54
BspHI TCATGA 1 cut(s) 481
BspLI GGNNCC 1 cut(s) 701
BspMAI CTGCAG 7 cut(s) 19, 189, 251, 273, 340, 456, 802
BspMI ACCTGC 4 cut(s) 85, 122, 205, 296
BspPI GGATC 2 cut(s) 479, 657
BsrFI RCCGGY 1 cut(s) 58
BsrGI TGTACA 1 cut(s) 583
BsrI ACTGG 4 cut(s) 436, 1019, 1025, 1059
BssAI RCCGGY 1 cut(s) 58
BssECI CCNNGG 3 cut(s) 370, 390, 1052
BssMI GATC 7 cut(s) 484, 598, 649, 712, 808, 959, 1083
BssNI GRCGYC 2 cut(s) 794, 1075
BssT1I CCWWGG 1 cut(s) 390
Bst2UI CCWGG 1 cut(s) 499
Bst4CI ACNGT 5 cut(s) 203, 592, 685, 729, 1046
BstACI GRCGYC 2 cut(s) 794, 1075
BstAPI GCANNNNNTGC 1 cut(s) 75
BstAUI TGTACA 1 cut(s) 583
BstC8I GCNNGC 8 cut(s) 45, 56, 60, 64, 104, 228, 251, 273
BstF5I GGATG 4 cut(s) 302, 351, 494, 755
BstFNI CGCG 1 cut(s) 54
BstHHI GCGC 3 cut(s) 54, 94, 305
BstKTI GATC 7 cut(s) 487, 601, 652, 715, 811, 962, 1086
BstMBI GATC 7 cut(s) 484, 598, 649, 712, 808, 959, 1083
BstMCI CGRYCG 2 cut(s) 405, 1052
BstNI CCWGG 1 cut(s) 499
BstNSI RCATGY 1 cut(s) 911
BstSCI CCNGG 3 cut(s) 497, 565, 993
BstSFI CTRYAG 8 cut(s) 15, 163, 185, 247, 269, 336, 452, 798
BstUI CGCG 1 cut(s) 54
BstX2I RGATCY 2 cut(s) 808, 959
BstYI RGATCY 2 cut(s) 808, 959
BstZI CGGCCG 2 cut(s) 402, 1049
BsuRI GGCC 7 cut(s) 58, 389, 404, 1040, 1051, 1058, 1065
BtgZI GCGATG 1 cut(s) 393
BtrI CACGTC 1 cut(s) 288
BtsCI GGATG 4 cut(s) 302, 351, 494, 755
BtsI GCAGTG 2 cut(s) 24, 449
BtsIMutI CAGTG 4 cut(s) 24, 449, 1032, 1066
BveI ACCTGC 4 cut(s) 85, 122, 205, 296
Cac8I GCNNGC 8 cut(s) 45, 56, 60, 64, 104, 228, 251, 273
CaiI CAGNNNCTG 1 cut(s) 75
CciI TCATGA 1 cut(s) 481
CciNI GCGGCCGC 1 cut(s) 402
CfoI GCGC 3 cut(s) 54, 94, 305
Cfr10I RCCGGY 1 cut(s) 58
Cfr13I GGNCC 2 cut(s) 56, 1056
CseI GACGC 1 cut(s) 1083
Csp6I GTAC 4 cut(s) 584, 730, 930, 1022
CviAII CATG 5 cut(s) 408, 414, 425, 482, 908
CviQI GTAC 4 cut(s) 584, 730, 930, 1022
DpnI GATC 7 cut(s) 486, 600, 651, 714, 810, 961, 1085
DpnII GATC 7 cut(s) 484, 598, 649, 712, 808, 959, 1083
EaeI YGGCCR 4 cut(s) 402, 1038, 1049, 1063
EagI CGGCCG 2 cut(s) 402, 1049
EclXI CGGCCG 2 cut(s) 402, 1049
Eco130I CCWWGG 1 cut(s) 390
Eco32I GATATC 1 cut(s) 466
Eco52I CGGCCG 2 cut(s) 402, 1049
EcoRII CCWGG 1 cut(s) 497
EcoRV GATATC 1 cut(s) 466
EcoT14I CCWWGG 1 cut(s) 390
ErhI CCWWGG 1 cut(s) 390
FaeI CATG 5 cut(s) 411, 417, 428, 485, 911
FaqI GGGAC 2 cut(s) 442, 967
FatI CATG 5 cut(s) 407, 413, 424, 481, 907
FauI CCCGC 4 cut(s) 47, 95, 219, 286
FbaI TGATCA 2 cut(s) 598, 1083
FokI GGATG 4 cut(s) 309, 358, 481, 762
FspBI CTAG 8 cut(s) 147, 342, 509, 573, 639, 666, 704, 950
FspI TGCGCA 1 cut(s) 304
GlaI GCGC 3 cut(s) 53, 93, 304
GsaI CCCAGC 2 cut(s) 210, 334
HaeIII GGCC 7 cut(s) 58, 389, 404, 1040, 1051, 1058, 1065
HapII CCGG 3 cut(s) 59, 566, 995
HgaI GACGC 1 cut(s) 1083
HhaI GCGC 3 cut(s) 54, 94, 305
Hin1I GRCGYC 2 cut(s) 794, 1075
Hin1II CATG 5 cut(s) 411, 417, 428, 485, 911
Hin6I GCGC 3 cut(s) 52, 92, 303
HinP1I GCGC 3 cut(s) 52, 92, 303
HinfI GANTC 1 cut(s) 662
HpaII CCGG 3 cut(s) 59, 566, 995
HphI GGTGA 3 cut(s) 683, 759, 857
Hpy166II GTNNAC 1 cut(s) 584
Hpy188I TCNGA 3 cut(s) 470, 792, 841
Hpy188III TCNNGA 2 cut(s) 482, 611
Hpy8I GTNNAC 1 cut(s) 584
Hpy99I CGWCG 1 cut(s) 796
HpyAV CCTTC 2 cut(s) 434, 548
HpyCH4III ACNGT 5 cut(s) 203, 592, 685, 729, 1046
HpyCH4IV ACGT 3 cut(s) 8, 287, 794
HpySE526I ACGT 3 cut(s) 8, 287, 794
Hsp92I GRCGYC 2 cut(s) 794, 1075
Hsp92II CATG 5 cut(s) 411, 417, 428, 485, 911
HspAI GCGC 3 cut(s) 52, 92, 303
KroI GCCGGC 1 cut(s) 58
KroNI GCCGGC 1 cut(s) 60
Ksp22I TGATCA 2 cut(s) 598, 1083
Kzo9I GATC 7 cut(s) 484, 598, 649, 712, 808, 959, 1083
LweI GCATC 2 cut(s) 740, 752
MaeI CTAG 8 cut(s) 147, 342, 509, 573, 639, 666, 704, 950
MaeII ACGT 3 cut(s) 8, 287, 794
MaeIII GTNAC 5 cut(s) 447, 671, 780, 845, 1067
MalI GATC 7 cut(s) 486, 600, 651, 714, 810, 961, 1085
MboI GATC 7 cut(s) 484, 598, 649, 712, 808, 959, 1083
MboII GAAGA 4 cut(s) 483, 803, 818, 880
MfeI CAATTG 1 cut(s) 1087
MflI RGATCY 2 cut(s) 808, 959
MlsI TGGCCA 1 cut(s) 1040
MluCI AATT 7 cut(s) 354, 383, 458, 521, 624, 945, 1087
MluNI TGGCCA 1 cut(s) 1040
MlyI GAGTC 1 cut(s) 671
MmeI TCCRAC 3 cut(s) 627, 688, 815
MnlI CCTC 9 cut(s) 152, 174, 250, 258, 320, 365, 858, 884, 1047
Mox20I TGGCCA 1 cut(s) 1040
MroNI GCCGGC 1 cut(s) 58
MroXI GAANNNNTTC 1 cut(s) 810
MscI TGGCCA 1 cut(s) 1040
MslI CAYNNNNRTG 2 cut(s) 412, 939
Msp20I TGGCCA 1 cut(s) 1040
MspA1I CMGCKG 2 cut(s) 30, 315
MspI CCGG 3 cut(s) 59, 566, 995
MspR9I CCNGG 3 cut(s) 499, 567, 995
MunI CAATTG 1 cut(s) 1087
MvaI CCWGG 1 cut(s) 499
MvnI CGCG 1 cut(s) 54
NaeI GCCGGC 1 cut(s) 60
NciI CCSGG 2 cut(s) 567, 995
NdeII GATC 7 cut(s) 484, 598, 649, 712, 808, 959, 1083
NgoMIV GCCGGC 1 cut(s) 58
NlaIII CATG 5 cut(s) 411, 417, 428, 485, 911
NlaIV GGNNCC 1 cut(s) 701
NmeAIII GCCGAG 2 cut(s) 395, 1077
NmuCI GTSAC 4 cut(s) 447, 671, 845, 1067
NotI GCGGCCGC 1 cut(s) 402
NsbI TGCGCA 1 cut(s) 304
NspI RCATGY 1 cut(s) 911
PagI TCATGA 1 cut(s) 481
PaqCI CACCTGC 1 cut(s) 85
PdiI GCCGGC 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 810
PfoI TCCNGGA 1 cut(s) 565
PleI GAGTC 1 cut(s) 670
PpsI GAGTC 1 cut(s) 670
PsiI TTATAA 1 cut(s) 690
Psp6I CCWGG 1 cut(s) 497
PspFI CCCAGC 2 cut(s) 206, 330
PspGI CCWGG 1 cut(s) 497
PspN4I GGNNCC 1 cut(s) 701
PspPI GGNCC 2 cut(s) 56, 1056
PstI CTGCAG 7 cut(s) 19, 189, 251, 273, 340, 456, 802
PstNI CAGNNNCTG 1 cut(s) 75
PsuI RGATCY 2 cut(s) 808, 959
PvuII CAGCTG 1 cut(s) 315
RsaI GTAC 4 cut(s) 585, 731, 931, 1023
RsaNI GTAC 4 cut(s) 584, 730, 930, 1022
RseI CAYNNNNRTG 2 cut(s) 412, 939
Sau3AI GATC 7 cut(s) 484, 598, 649, 712, 808, 959, 1083
Sau96I GGNCC 2 cut(s) 56, 1056
ScaI AGTACT 1 cut(s) 931
SchI GAGTC 1 cut(s) 671
ScrFI CCNGG 3 cut(s) 499, 567, 995
SfaNI GCATC 2 cut(s) 740, 752
SfcI CTRYAG 8 cut(s) 15, 163, 185, 247, 269, 336, 452, 798
SmiMI CAYNNNNRTG 2 cut(s) 412, 939
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
SpeI ACTAGT 1 cut(s) 572
Sse9I AATT 7 cut(s) 354, 383, 458, 521, 624, 945, 1087
SspMI CTAG 8 cut(s) 147, 342, 509, 573, 639, 666, 704, 950
StyD4I CCNGG 3 cut(s) 497, 565, 993
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 5 cut(s) 203, 592, 685, 729, 1046
TaiI ACGT 3 cut(s) 11, 290, 797
TaqI TCGA 1 cut(s) 817
TasI AATT 7 cut(s) 354, 383, 458, 521, 624, 945, 1087
TatI WGTACW 2 cut(s) 583, 929
TauI GCSGC 4 cut(s) 33, 323, 404, 407
TscAI CASTG 4 cut(s) 24, 456, 1032, 1066
TseFI GTSAC 4 cut(s) 447, 671, 845, 1067
Tsp45I GTSAC 4 cut(s) 447, 671, 845, 1067
TspDTI ATGAA 5 cut(s) 396, 470, 567, 636, 803
TspRI CASTG 4 cut(s) 24, 456, 1032, 1066
XapI RAATTY 1 cut(s) 458
XceI RCATGY 1 cut(s) 911
XcmI CCANNNNNNNNNTGG 1 cut(s) 743
XmnI GAANNNNTTC 1 cut(s) 810
XspI CTAG 8 cut(s) 147, 342, 509, 573, 639, 666, 704, 950
ZraI GACGTC 1 cut(s) 795
ZrmI AGTACT 1 cut(s) 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.