pycom17g26720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0010011
Physical Location & Seq
Forward (+)
25009857 .. 25010549
693 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g26720.1

Sequence Viewer

Length: 693 bp
ATGCAGAGAAGAAAGCCGAGGGGAAGCTTCAACGACATTGATCGATCGGCAGTAGTAAAGGCAGCTGCATGGGCATGGTTCGAGCACTGTTCGGGTTCCGACGGCAAGTCGATCATGCCGGAATTCGATTTAACAAGGACTTGTCACGCTCCTTATAAACCGTCCAGGTACAAGCTAGAAGCTATGAAAAATATAGCCAATATTAGCAGTAAAGAAAGTGTTACTGCATCTAGTAACCCGCTTCTTGATCCATATGAAATTGAAAGTATATCCCGGAAATTGGATCAACTTATTGAATTTAGTGGTGACAGAGGCAAACTTCGTAAGGGTTTTCTAGTAGGAGACGTTCTTGGTATAAAGAACATGAAAGAGAGCGGGAGAGGTTCGGACTCTGACCTCGAATACAGAGATAAACGCTCTTTACCACTGAAGCCACATGTCCCCGAAGACATTCGTCAACAAGAATGTGTGCCGCTCGTGGATGGTAACACGAACGGGAGGAAGAAAAAGAACAAGAACGTTAGAGGAGGGTTTTGGCATAGGCATGCGGCGATATGTGGCAGAAGTGAAGATGTTGTAAACACACAAGCTTTCGTGGTTCATCGTCGGCGGCCGGAAAAGGGTGTATATGTGCGTACCGGTGGACCGGTGGTTAAGACGCGCGGCGAACTTTCGGCCACGGAGGCCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

231

Amino Acids

25.82

Weight (kDa)

9.71

Isoelectric Point (pI)

47.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 156
AccBSI CCGCTC 2 cut(s) 375, 475
AccII CGCG 2 cut(s) 661, 663
AciI CCGC 6 cut(s) 239, 375, 473, 548, 610, 663
AclI AACGTT 1 cut(s) 519
AclWI GGATC 2 cut(s) 242, 291
AcoI YGGCCR 2 cut(s) 611, 675
AcsI RAATTY 2 cut(s) 122, 296
AcuI CTGAAG 1 cut(s) 449
AfaI GTAC 2 cut(s) 170, 637
AfiI CCNNNNNNNGG 2 cut(s) 280, 620
AflIII ACRYGT 1 cut(s) 436
AgeI ACCGGT 2 cut(s) 638, 646
AgsI TTSAA 3 cut(s) 31, 263, 296
AhdI GACNNNNNGTC 1 cut(s) 106
AjnI CCWGG 1 cut(s) 164
AluBI AGCT 5 cut(s) 27, 65, 175, 182, 590
AluI AGCT 5 cut(s) 27, 65, 175, 182, 590
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 1 cut(s) 336
AlwI GGATC 2 cut(s) 242, 291
AoxI GGCC 3 cut(s) 611, 675, 684
ApeKI GCWGC 2 cut(s) 62, 65
ApoI RAATTY 2 cut(s) 122, 296
AsiGI ACCGGT 2 cut(s) 638, 646
Asp700I GAANNNNTTC 1 cut(s) 450
AspLEI GCGC 1 cut(s) 663
AspS9I GGNCC 1 cut(s) 644
AsuC2I CCSGG 1 cut(s) 274
AsuHPI GGTGA 1 cut(s) 317
AvaII GGWCC 1 cut(s) 644
BauI CACGAG 1 cut(s) 476
BbsI GAAGAC 1 cut(s) 453
Bbv12I GWGCWC 1 cut(s) 87
BbvI GCAGC 2 cut(s) 52, 74
BccI CCATC 1 cut(s) 476
BceAI ACGGC 1 cut(s) 118
BciT130I CCWGG 1 cut(s) 166
BcnI CCSGG 1 cut(s) 274
BcoDI GTCTC 1 cut(s) 336
BfaI CTAG 3 cut(s) 176, 231, 335
BglI GCCNNNNNGGC 1 cut(s) 683
BisI GCNGC 6 cut(s) 63, 66, 473, 549, 611, 664
BlsI GCNGC 6 cut(s) 64, 67, 474, 550, 612, 665
Bme1390I CCNGG 2 cut(s) 166, 274
Bme18I GGWCC 1 cut(s) 644
BmeRI GACNNNNNGTC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 644
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 2 cut(s) 166, 274
BmsI GCATC 1 cut(s) 236
BoxI GACNNNNGTC 1 cut(s) 453
BpiI GAAGAC 1 cut(s) 453
BpuMI CCSGG 1 cut(s) 274
Bsa29I ATCGAT 1 cut(s) 43
BsaJI CCNNGG 2 cut(s) 17, 678
BsaWI WCCGGW 2 cut(s) 638, 646
BsaXI ACNNNNNCTCC 1 cut(s) 674
Bsc4I CCNNNNNNNGG 2 cut(s) 280, 620
Bse118I RCCGGY 2 cut(s) 638, 646
BseBI CCWGG 1 cut(s) 166
BseCI ATCGAT 1 cut(s) 43
BseDI CCNNGG 2 cut(s) 17, 678
BseGI GGATG 1 cut(s) 487
BseLI CCNNNNNNNGG 2 cut(s) 280, 620
BseRI GAGGAG 1 cut(s) 540
BseX3I CGGCCG 1 cut(s) 611
BseXI GCAGC 2 cut(s) 52, 74
Bsh1236I CGCG 2 cut(s) 661, 663
Bsh1285I CGRYCG 2 cut(s) 47, 614
BshFI GGCC 3 cut(s) 613, 677, 686
BshTI ACCGGT 2 cut(s) 638, 646
BshVI ATCGAT 1 cut(s) 43
BsiEI CGRYCG 2 cut(s) 47, 614
BsiHKAI GWGCWC 1 cut(s) 87
BsiSI CCGG 5 cut(s) 119, 274, 614, 639, 647
BslFI GGGAC 1 cut(s) 425
BslI CCNNNNNNNGG 2 cut(s) 280, 620
BsmAI GTCTC 1 cut(s) 336
BsmBI CGTCTC 1 cut(s) 336
BsmFI GGGAC 1 cut(s) 425
BsnI GGCC 3 cut(s) 613, 677, 686
Bsp1286I GDGCHC 1 cut(s) 87
Bsp143I GATC 5 cut(s) 40, 44, 111, 247, 283
BspACI CCGC 6 cut(s) 239, 375, 473, 548, 610, 663
BspANI GGCC 3 cut(s) 613, 677, 686
BspDI ATCGAT 1 cut(s) 43
BspFNI CGCG 2 cut(s) 661, 663
BspLI GGNNCC 1 cut(s) 97
BspPI GGATC 2 cut(s) 242, 291
BsrBI CCGCTC 2 cut(s) 375, 475
BsrFI RCCGGY 2 cut(s) 638, 646
BssAI RCCGGY 2 cut(s) 638, 646
BssECI CCNNGG 2 cut(s) 17, 678
BssMI GATC 5 cut(s) 40, 44, 111, 247, 283
BssSI CACGAG 1 cut(s) 476
Bst2BI CACGAG 1 cut(s) 476
Bst2UI CCWGG 1 cut(s) 166
Bst4CI ACNGT 2 cut(s) 89, 162
BstC8I GCNNGC 1 cut(s) 546
BstDSI CCRYGG 1 cut(s) 678
BstF5I GGATG 1 cut(s) 487
BstFNI CGCG 2 cut(s) 661, 663
BstHHI GCGC 1 cut(s) 663
BstKTI GATC 5 cut(s) 43, 47, 114, 250, 286
BstMAI GTCTC 1 cut(s) 336
BstMBI GATC 5 cut(s) 40, 44, 111, 247, 283
BstMCI CGRYCG 2 cut(s) 47, 614
BstMWI GCNNNNNNNGC 2 cut(s) 71, 683
BstNI CCWGG 1 cut(s) 166
BstNSI RCATGY 2 cut(s) 440, 548
BstPAI GACNNNNGTC 1 cut(s) 453
BstSCI CCNGG 2 cut(s) 164, 272
BstUI CGCG 2 cut(s) 661, 663
BstV1I GCAGC 2 cut(s) 52, 74
BstV2I GAAGAC 1 cut(s) 453
BstZI CGGCCG 1 cut(s) 611
Bsu15I ATCGAT 1 cut(s) 43
BsuRI GGCC 3 cut(s) 613, 677, 686
BsuTUI ATCGAT 1 cut(s) 43
BtgI CCRYGG 1 cut(s) 678
BtsCI GGATG 1 cut(s) 487
BtsIMutI CAGTG 2 cut(s) 85, 425
Cac8I GCNNGC 1 cut(s) 546
CfoI GCGC 1 cut(s) 663
Cfr10I RCCGGY 2 cut(s) 638, 646
Cfr13I GGNCC 1 cut(s) 644
ClaI ATCGAT 1 cut(s) 43
CseI GACGC 1 cut(s) 667
Csp6I GTAC 2 cut(s) 169, 636
CspAI ACCGGT 2 cut(s) 638, 646
CviAII CATG 6 cut(s) 69, 75, 115, 364, 437, 545
CviQI GTAC 2 cut(s) 169, 636
DpnI GATC 5 cut(s) 42, 46, 113, 249, 285
DpnII GATC 5 cut(s) 40, 44, 111, 247, 283
DriI GACNNNNNGTC 1 cut(s) 106
EaeI YGGCCR 2 cut(s) 611, 675
EagI CGGCCG 1 cut(s) 611
Eam1105I GACNNNNNGTC 1 cut(s) 106
EclXI CGGCCG 1 cut(s) 611
Eco147I AGGCCT 1 cut(s) 686
Eco47I GGWCC 1 cut(s) 644
Eco52I CGGCCG 1 cut(s) 611
Eco57I CTGAAG 1 cut(s) 449
EcoRI GAATTC 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 164
Esp3I CGTCTC 1 cut(s) 336
FaeI CATG 6 cut(s) 72, 78, 118, 367, 440, 548
FaqI GGGAC 1 cut(s) 425
FatI CATG 6 cut(s) 68, 74, 114, 363, 436, 544
FauI CCCGC 2 cut(s) 246, 368
FauNDI CATATG 1 cut(s) 253
Fnu4HI GCNGC 6 cut(s) 63, 66, 473, 549, 611, 664
FokI GGATG 1 cut(s) 494
Fsp4HI GCNGC 6 cut(s) 63, 66, 473, 549, 611, 664
FspBI CTAG 3 cut(s) 176, 231, 335
GlaI GCGC 1 cut(s) 662
GluI GCNGC 6 cut(s) 63, 66, 473, 549, 611, 664
HaeIII GGCC 3 cut(s) 613, 677, 686
HapII CCGG 5 cut(s) 119, 274, 614, 639, 647
HgaI GACGC 1 cut(s) 667
HhaI GCGC 1 cut(s) 663
Hin1II CATG 6 cut(s) 72, 78, 118, 367, 440, 548
Hin6I GCGC 1 cut(s) 661
HinP1I GCGC 1 cut(s) 661
HincII GTYRAC 1 cut(s) 458
HindII GTYRAC 1 cut(s) 458
HindIII AAGCTT 2 cut(s) 25, 588
HinfI GANTC 1 cut(s) 389
HpaII CCGG 5 cut(s) 119, 274, 614, 639, 647
HphI GGTGA 1 cut(s) 317
Hpy166II GTNNAC 3 cut(s) 458, 580, 644
Hpy188I TCNGA 3 cut(s) 100, 388, 394
Hpy188III TCNNGA 1 cut(s) 245
Hpy8I GTNNAC 3 cut(s) 458, 580, 644
Hpy99I CGWCG 2 cut(s) 104, 609
HpyCH4III ACNGT 2 cut(s) 89, 162
HpyCH4IV ACGT 2 cut(s) 345, 519
HpyCH4V TGCA 3 cut(s) 4, 68, 227
HpyF10VI GCNNNNNNNGC 2 cut(s) 71, 683
HpySE526I ACGT 2 cut(s) 345, 519
Hsp92II CATG 6 cut(s) 72, 78, 118, 367, 440, 548
HspAI GCGC 1 cut(s) 661
Kzo9I GATC 5 cut(s) 40, 44, 111, 247, 283
LmnI GCTCC 1 cut(s) 154
LpnPI CCDG 7 cut(s) 132, 151, 178, 287, 627, 652, 660
Lsp1109I GCAGC 2 cut(s) 52, 74
LweI GCATC 1 cut(s) 236
MaeI CTAG 3 cut(s) 176, 231, 335
MaeII ACGT 2 cut(s) 345, 519
MaeIII GTNAC 5 cut(s) 143, 220, 233, 305, 485
MalI GATC 5 cut(s) 42, 46, 113, 249, 285
MbiI CCGCTC 2 cut(s) 375, 475
MboI GATC 5 cut(s) 40, 44, 111, 247, 283
MboII GAAGA 4 cut(s) 21, 458, 514, 581
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 4 cut(s) 122, 258, 278, 296
MlyI GAGTC 1 cut(s) 383
MmeI TCCRAC 1 cut(s) 123
MnlI CCTC 8 cut(s) 12, 305, 374, 407, 492, 518, 521, 676
MroXI GAANNNNTTC 1 cut(s) 450
MseI TTAA 2 cut(s) 131, 654
MslI CAYNNNNRTG 2 cut(s) 73, 543
MspA1I CMGCKG 1 cut(s) 65
MspI CCGG 5 cut(s) 119, 274, 614, 639, 647
MspR9I CCNGG 2 cut(s) 166, 274
MvaI CCWGG 1 cut(s) 166
MvnI CGCG 2 cut(s) 661, 663
MwoI GCNNNNNNNGC 2 cut(s) 71, 683
NciI CCSGG 1 cut(s) 274
NdeI CATATG 1 cut(s) 253
NdeII GATC 5 cut(s) 40, 44, 111, 247, 283
NlaIII CATG 6 cut(s) 72, 78, 118, 367, 440, 548
NlaIV GGNNCC 1 cut(s) 97
NmeAIII GCCGAG 1 cut(s) 42
NmuCI GTSAC 2 cut(s) 143, 305
NspI RCATGY 2 cut(s) 440, 548
PaeI GCATGC 1 cut(s) 548
PceI AGGCCT 1 cut(s) 686
PciI ACATGT 1 cut(s) 436
PdmI GAANNNNTTC 1 cut(s) 450
PfoI TCCNGGA 1 cut(s) 272
PinAI ACCGGT 2 cut(s) 638, 646
PkrI GCNGC 6 cut(s) 64, 67, 474, 550, 612, 665
Ple19I CGATCG 1 cut(s) 47
PleI GAGTC 1 cut(s) 383
PpsI GAGTC 1 cut(s) 383
PscI ACATGT 1 cut(s) 436
PshAI GACNNNNGTC 1 cut(s) 453
PsiI TTATAA 1 cut(s) 156
Psp1406I AACGTT 1 cut(s) 519
Psp6I CCWGG 1 cut(s) 164
PspGI CCWGG 1 cut(s) 164
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 1 cut(s) 644
PsrI GAACNNNNNNTAC 2 cut(s) 330, 362
PvuI CGATCG 1 cut(s) 47
PvuII CAGCTG 1 cut(s) 65
RsaI GTAC 2 cut(s) 170, 637
RsaNI GTAC 2 cut(s) 169, 636
RseI CAYNNNNRTG 2 cut(s) 73, 543
SaqAI TTAA 2 cut(s) 131, 654
SatI GCNGC 6 cut(s) 63, 66, 473, 549, 611, 664
Sau3AI GATC 5 cut(s) 40, 44, 111, 247, 283
Sau96I GGNCC 1 cut(s) 644
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 2 cut(s) 166, 274
SduI GDGCHC 1 cut(s) 87
SfaNI GCATC 1 cut(s) 236
SfiI GGCCNNNNNGGCC 1 cut(s) 683
SinI GGWCC 1 cut(s) 644
SmiMI CAYNNNNRTG 2 cut(s) 73, 543
SphI GCATGC 1 cut(s) 548
Sse9I AATT 4 cut(s) 122, 258, 278, 296
SseBI AGGCCT 1 cut(s) 686
SsiI CCGC 6 cut(s) 239, 375, 473, 548, 610, 663
SspI AATATT 1 cut(s) 202
SspMI CTAG 3 cut(s) 176, 231, 335
StuI AGGCCT 1 cut(s) 686
StyD4I CCNGG 2 cut(s) 164, 272
TaaI ACNGT 2 cut(s) 89, 162
TaiI ACGT 2 cut(s) 348, 522
TaqI TCGA 5 cut(s) 43, 81, 110, 126, 399
TasI AATT 4 cut(s) 122, 258, 278, 296
TauI GCSGC 4 cut(s) 475, 551, 613, 666
Tru1I TTAA 2 cut(s) 131, 654
Tru9I TTAA 2 cut(s) 131, 654
TscAI CASTG 2 cut(s) 92, 432
TseFI GTSAC 2 cut(s) 143, 305
TseI GCWGC 2 cut(s) 62, 65
Tsp45I GTSAC 2 cut(s) 143, 305
TspDTI ATGAA 4 cut(s) 200, 270, 380, 590
TspRI CASTG 2 cut(s) 92, 432
VpaK11BI GGWCC 1 cut(s) 644
XapI RAATTY 2 cut(s) 122, 296
XceI RCATGY 2 cut(s) 440, 548
XmnI GAANNNNTTC 1 cut(s) 450
XspI CTAG 3 cut(s) 176, 231, 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.