MD10G1044200.v1.1

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011601
Physical Location & Seq
Forward (+)
5943430 .. 5944074
645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1044200.v1.1.491

Sequence Viewer

Length: 624 bp
ATGGACAACAACTCCATCATCCAAGACCTCATTGAAAGCACTGAAGAACACGATGACGACCCAAATAACGAATCCAATTTCATGTCTGCTATGAACGCAATACTAAGTGGCACTGCACGTCTCAACGTTCTCCTGCCCACGGCCCCCGTTCTTTCCTTCACCATTTTCACTCCTCTCTTCACCAACGACGGTGTATGCACCCCTCTCAACCGCTGGCTGACGGGTTTCTTTATCGCTCTTTCAGCAGCATCCTGCGTCTTCTTTACACTCACCGATAGCTTCAAAACAGCGACTGGCAGGTTATACTATGGAGTGGCCACATTTCGGGGGATACGGACTTTCAATGGTGGCCGGAAAAAGCCACATTTGCCCTCCGATTATAGGTTGAGATGGGCGGATCTCTTCCATGCATCACTTTCGGTAGTCGCGTTTCTGGCGTTTGCAGCTTTGCATTATGATGTCGTGGGTTGTTATTATCCGGCAATGCCTCGGAGGGTGACCAACGTTCTTCCTCTTGTGGTAGGGTTTGTAGTTAGTGTTTTGTTTGTGTTGTTTCCTTCTAAGAGAAGGGGAATTGGGTATCCTTTCTTGCTGCAGAGAGAGGCTTTTTACTCTAGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF679

Protein Analysis

208

Amino Acids

23.17

Weight (kDa)

8.73

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 37 - 194 2.8e-56 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 288
AccII CGCG 1 cut(s) 428
AciI CCGC 2 cut(s) 211, 395
AclI AACGTT 2 cut(s) 126, 504
AclWI GGATC 1 cut(s) 405
AcoI YGGCCR 2 cut(s) 315, 349
AcuI CTGAAG 1 cut(s) 63
AfiI CCNNNNNNNGG 3 cut(s) 139, 324, 381
AgsI TTSAA 3 cut(s) 35, 283, 343
AjiI CACGTC 1 cut(s) 119
AluBI AGCT 2 cut(s) 279, 446
AluI AGCT 2 cut(s) 279, 446
Alw26I GTCTC 1 cut(s) 125
AlwI GGATC 1 cut(s) 405
AlwNI CAGNNNCTG 1 cut(s) 293
AoxI GGCC 3 cut(s) 141, 315, 349
ApeKI GCWGC 3 cut(s) 245, 443, 592
AspS9I GGNCC 1 cut(s) 142
AsuHPI GGTGA 4 cut(s) 151, 172, 262, 508
BalI TGGCCA 1 cut(s) 317
BbsI GAAGAC 1 cut(s) 250
BbvI GCAGC 3 cut(s) 257, 455, 579
BccI CCATC 2 cut(s) 23, 384
BceAI ACGGC 1 cut(s) 156
BcgI CGANNNNNNTGC 2 cut(s) 399, 433
BciVI GTATCC 2 cut(s) 324, 591
BcoDI GTCTC 1 cut(s) 125
BfaI CTAG 1 cut(s) 615
BfmI CTRYAG 1 cut(s) 593
BfuAI ACCTGC 1 cut(s) 288
BfuI GTATCC 2 cut(s) 324, 591
BisI GCNGC 3 cut(s) 246, 444, 593
BlsI GCNGC 3 cut(s) 247, 445, 594
BmgBI CACGTC 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 142
BmiI GGNNCC 1 cut(s) 144
BmsI GCATC 2 cut(s) 257, 419
BpiI GAAGAC 1 cut(s) 250
BsaJI CCNNGG 2 cut(s) 138, 488
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 324, 381
Bse1I ACTGG 1 cut(s) 298
Bse3DI GCAATG 1 cut(s) 489
BseDI CCNNGG 2 cut(s) 138, 488
BseGI GGATG 2 cut(s) 18, 248
BseLI CCNNNNNNNGG 3 cut(s) 139, 324, 381
BseMI GCAATG 1 cut(s) 489
BseNI ACTGG 1 cut(s) 298
BseRI GAGGAG 1 cut(s) 162
BseXI GCAGC 3 cut(s) 257, 455, 579
BsgI GTGCAG 1 cut(s) 99
Bsh1236I CGCG 1 cut(s) 428
BshFI GGCC 3 cut(s) 143, 317, 351
BsiSI CCGG 2 cut(s) 352, 479
BslI CCNNNNNNNGG 3 cut(s) 139, 324, 381
BsmAI GTCTC 1 cut(s) 125
BsmBI CGTCTC 1 cut(s) 125
BsnI GGCC 3 cut(s) 143, 317, 351
Bsp143I GATC 1 cut(s) 397
BspACI CCGC 2 cut(s) 211, 395
BspANI GGCC 3 cut(s) 143, 317, 351
BspFNI CGCG 1 cut(s) 428
BspLI GGNNCC 1 cut(s) 144
BspMAI CTGCAG 1 cut(s) 597
BspMI ACCTGC 1 cut(s) 288
BspPI GGATC 1 cut(s) 405
BsrDI GCAATG 1 cut(s) 489
BsrI ACTGG 1 cut(s) 298
BssECI CCNNGG 2 cut(s) 138, 488
BssMI GATC 1 cut(s) 397
Bst4CI ACNGT 1 cut(s) 191
Bst6I CTCTTC 2 cut(s) 182, 407
BstC8I GCNNGC 1 cut(s) 215
BstDEI CTNAG 2 cut(s) 104, 561
BstDSI CCRYGG 1 cut(s) 138
BstEII GGTNACC 1 cut(s) 496
BstF5I GGATG 2 cut(s) 18, 248
BstFNI CGCG 1 cut(s) 428
BstKTI GATC 1 cut(s) 400
BstMAI GTCTC 1 cut(s) 125
BstMBI GATC 1 cut(s) 397
BstMWI GCNNNNNNNGC 5 cut(s) 95, 242, 367, 434, 443
BstPI GGTNACC 1 cut(s) 496
BstSFI CTRYAG 1 cut(s) 593
BstUI CGCG 1 cut(s) 428
BstV1I GCAGC 3 cut(s) 257, 455, 579
BstV2I GAAGAC 1 cut(s) 250
BstX2I RGATCY 1 cut(s) 397
BstYI RGATCY 1 cut(s) 397
BsuI GTATCC 2 cut(s) 324, 591
BsuRI GGCC 3 cut(s) 143, 317, 351
BtgI CCRYGG 1 cut(s) 138
BtrI CACGTC 1 cut(s) 119
BtsCI GGATG 2 cut(s) 18, 248
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 2 cut(s) 39, 111
BveI ACCTGC 1 cut(s) 288
Cac8I GCNNGC 1 cut(s) 215
CaiI CAGNNNCTG 1 cut(s) 293
Cfr13I GGNCC 1 cut(s) 142
CseI GACGC 1 cut(s) 244
CviAII CATG 2 cut(s) 82, 407
CviJI RGCY 8 cut(s) 143, 217, 279, 317, 351, 361, 446, 605
CviKI_1 RGCY 8 cut(s) 143, 217, 279, 317, 351, 361, 446, 605
DdeI CTNAG 2 cut(s) 104, 561
DpnI GATC 1 cut(s) 399
DpnII GATC 1 cut(s) 397
EaeI YGGCCR 2 cut(s) 315, 349
Eam1104I CTCTTC 2 cut(s) 182, 407
EarI CTCTTC 2 cut(s) 182, 407
EciI GGCGGA 1 cut(s) 410
Eco57I CTGAAG 1 cut(s) 63
Eco91I GGTNACC 1 cut(s) 496
EcoO65I GGTNACC 1 cut(s) 496
EcoT22I ATGCAT 1 cut(s) 412
Esp3I CGTCTC 1 cut(s) 125
FaeI CATG 2 cut(s) 85, 410
FaiI YATR 8 cut(s) 83, 92, 196, 304, 309, 381, 408, 456
FatI CATG 2 cut(s) 81, 406
Fnu4HI GCNGC 3 cut(s) 246, 444, 593
FokI GGATG 2 cut(s) 5, 235
Fsp4HI GCNGC 3 cut(s) 246, 444, 593
FspBI CTAG 1 cut(s) 615
GluI GCNGC 3 cut(s) 246, 444, 593
HaeIII GGCC 3 cut(s) 143, 317, 351
HapII CCGG 2 cut(s) 352, 479
HgaI GACGC 1 cut(s) 244
Hin1II CATG 2 cut(s) 85, 410
HinfI GANTC 1 cut(s) 71
HpaII CCGG 2 cut(s) 352, 479
HphI GGTGA 4 cut(s) 151, 172, 262, 508
Hpy188I TCNGA 2 cut(s) 376, 492
Hpy188III TCNNGA 1 cut(s) 615
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 3 cut(s) 166, 561, 567
HpyCH4III ACNGT 1 cut(s) 191
HpyCH4IV ACGT 3 cut(s) 118, 126, 504
HpyCH4V TGCA 6 cut(s) 116, 198, 410, 443, 451, 595
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 242, 367, 434, 443
HpyF3I CTNAG 2 cut(s) 104, 561
HpySE526I ACGT 3 cut(s) 118, 126, 504
Hsp92II CATG 2 cut(s) 85, 410
Kzo9I GATC 1 cut(s) 397
LpnPI CCDG 8 cut(s) 146, 199, 265, 279, 283, 365, 419, 492
Lsp1109I GCAGC 3 cut(s) 257, 455, 579
LweI GCATC 2 cut(s) 257, 419
MaeI CTAG 1 cut(s) 615
MaeII ACGT 3 cut(s) 118, 126, 504
MaeIII GTNAC 1 cut(s) 496
MalI GATC 1 cut(s) 399
MboI GATC 1 cut(s) 397
MboII GAAGA 5 cut(s) 56, 169, 250, 394, 500
MflI RGATCY 1 cut(s) 397
MlsI TGGCCA 1 cut(s) 317
MluCI AATT 2 cut(s) 76, 573
MluNI TGGCCA 1 cut(s) 317
MnlI CCTC 8 cut(s) 38, 183, 213, 382, 486, 498, 522, 595
Mox20I TGGCCA 1 cut(s) 317
Mph1103I ATGCAT 1 cut(s) 412
MscI TGGCCA 1 cut(s) 317
MslI CAYNNNNRTG 1 cut(s) 456
Msp20I TGGCCA 1 cut(s) 317
MspA1I CMGCKG 1 cut(s) 213
MspI CCGG 2 cut(s) 352, 479
MvnI CGCG 1 cut(s) 428
MwoI GCNNNNNNNGC 5 cut(s) 95, 242, 367, 434, 443
NdeII GATC 1 cut(s) 397
NlaIII CATG 2 cut(s) 85, 410
NlaIV GGNNCC 1 cut(s) 144
NmuCI GTSAC 1 cut(s) 496
NsiI ATGCAT 1 cut(s) 412
PcsI WCGNNNNNNNCGW 1 cut(s) 425
PfeI GAWTC 1 cut(s) 71
PkrI GCNGC 3 cut(s) 247, 445, 594
Psp1406I AACGTT 2 cut(s) 126, 504
PspEI GGTNACC 1 cut(s) 496
PspN4I GGNNCC 1 cut(s) 144
PspPI GGNCC 1 cut(s) 142
PstI CTGCAG 1 cut(s) 597
PstNI CAGNNNCTG 1 cut(s) 293
PsuI RGATCY 1 cut(s) 397
RseI CAYNNNNRTG 1 cut(s) 456
SatI GCNGC 3 cut(s) 246, 444, 593
Sau3AI GATC 1 cut(s) 397
Sau96I GGNCC 1 cut(s) 142
SetI ASST 8 cut(s) 30, 121, 129, 281, 302, 386, 448, 507
SfaNI GCATC 2 cut(s) 257, 419
SfcI CTRYAG 1 cut(s) 593
SmiMI CAYNNNNRTG 1 cut(s) 456
Sse9I AATT 2 cut(s) 76, 573
SsiI CCGC 2 cut(s) 211, 395
SspMI CTAG 1 cut(s) 615
TaaI ACNGT 1 cut(s) 191
TaiI ACGT 3 cut(s) 121, 129, 507
TasI AATT 2 cut(s) 76, 573
TfiI GAWTC 1 cut(s) 71
TscAI CASTG 2 cut(s) 46, 118
TseFI GTSAC 1 cut(s) 496
TseI GCWGC 3 cut(s) 245, 443, 592
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 2 cut(s) 70, 107
TspGWI ACGGA 1 cut(s) 349
TspRI CASTG 2 cut(s) 46, 118
XbaI TCTAGA 1 cut(s) 614
XspI CTAG 1 cut(s) 615
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.