MD10G1114300.v1.1

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007184
Physical Location & Seq
Forward (+)
18744504 .. 18748152
3649 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1114300.v1.1.491

Sequence Viewer

Length: 1284 bp
ATGGCGGTGGAGTACGAGTGCTGCACCAGCGGATTCTTCATTCACATAGTGGTCATCGTGCTGCTGGTGCTGTTCGCGGGGCTAATGTCCGGCCTCACCTTGGGCCTCATGTCCATGAGCCTCGTCGATCTCGAGGTCCTCGCCAAGTCCGGGACGCCGAAGGATCGCAACCACGCCGCGAAGATATTACCAGTTGTCAAAAAACAGCATCTGTTGCTTTGCACCCTGCTGATTTGCAATGCTGCTGCGATGGAGGCACTCCCCATTTTTCTTGATGGTTTGGTGACAGCTTGGGGTGCAATCCTCATTTCGGTGACACTGATTCTTCTGTTTGGTGAGATTATACCACAATCTGTTTGTTCCCGATATGGTTTGGCTATTGGTGCAACAGTGGCACCATTTGTCCGTGTTCTTGTTTGGGTCTGCTTTCCGGTTGCATATCCTATTAGCAAGCTATTAGACTATCTGTTGGGCCATGGACATGTAGCTCTATTTCGCAGAGCTGAGTTGAAAACGCTTGTAGATATGCATGGTAATGAGGCTGGAAAAGGTGGAGAGTTGACACATGATGAAACAACCATTATTGCCGGAGCACTTGAACTCTCTGAGAAAACAGCTAGAGATGCCATGACTCCCATAGCTGAAACTTTTGCAATCGATATCAATGCCAAGCTTGACAGGAATTTGATGAATCTAATATTGGAGAAAGGGCATAGCAGAGTACCGGTTTATTATGAGGAGCCTACGAATATTATTGGACTCATCCTGGTAAAAAATTTGTTAACGGTGAACCCAGAAGATGAAGTGCCTGTAAAGAATGTCACTATACGCAAGATTCCAAGGGTTCCAGAAACGTTGCCCTTATATGACATATTGAACGAGTTCCAGAAAGGTCACAGTCACATGGCTATTGTTGTAAGAAGGTGCAATAAGAACACGGAGCAGACTAATGGCAATCCGGATGACAATCCAGTGAAAGAAGTGAAAGTCGACATTGACGGTGAAAAGCCTCTTCATGAGAAGTTGAAGAGCAAAAGATCACTACAGAAGTGGAAGAGCTTTACAAATACAAACAGTGCAAATAATTCATACAGGAGTGGGTCCAGAAGCAAGAAATGGACAAAGGAGATATATTCAGACATTCTGCAGATTGATAGTGAGCCACTCCCCAAGCTCCCTGAAGAGGAAGAGGCTGTTGGCATCATAACAATGGAAGATGTTATCGAAGAACTTTTACAGGAGGAGATTTTCGATGAGACTGACCATCAGTTTGAGGACTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

428

Amino Acids

47.48

Weight (kDa)

5.4

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 18 - 189 4.7e-35 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 394
AccI GTMKAC 1 cut(s) 990
AccII CGCG 2 cut(s) 77, 179
AccIII TCCGGA 1 cut(s) 958
AciI CCGC 4 cut(s) 5, 30, 77, 177
AclI AACGTT 1 cut(s) 854
AclWI GGATC 1 cut(s) 171
AcsI RAATTY 2 cut(s) 682, 775
AcuI CTGAAG 1 cut(s) 1200
AcyI GRCGYC 1 cut(s) 155
AdeI CACNNNGTG 1 cut(s) 49
AfaI GTAC 2 cut(s) 14, 723
AfiI CCNNNNNNNGG 4 cut(s) 100, 150, 310, 1183
AflIII ACRYGT 1 cut(s) 481
AgeI ACCGGT 1 cut(s) 724
AgsI TTSAA 4 cut(s) 511, 599, 877, 1027
AjnI CCWGG 1 cut(s) 765
AjuI GAANNNNNNNTTGG 4 cut(s) 683, 715, 1179, 1211
AluBI AGCT 9 cut(s) 290, 454, 488, 503, 617, 641, 673, 1059, 1174
AluI AGCT 9 cut(s) 290, 454, 488, 503, 617, 641, 673, 1059, 1174
Alw21I GWGCWC 1 cut(s) 595
Alw26I GTCTC 1 cut(s) 1250
AlwI GGATC 1 cut(s) 171
AlwNI CAGNNNCTG 1 cut(s) 211
Ama87I CYCGRG 1 cut(s) 131
Aor13HI TCCGGA 1 cut(s) 958
AoxI GGCC 3 cut(s) 91, 103, 472
ApeKI GCWGC 4 cut(s) 21, 61, 242, 245
ApoI RAATTY 2 cut(s) 682, 775
ArsI GACNNNNNNTTYG 1 cut(s) 1253
AsiGI ACCGGT 1 cut(s) 724
Asp700I GAANNNNTTC 1 cut(s) 881
AspS9I GGNCC 4 cut(s) 103, 136, 472, 1101
AsuC2I CCSGG 1 cut(s) 151
AsuHPI GGTGA 6 cut(s) 88, 295, 325, 347, 799, 1013
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 2 cut(s) 136, 1101
BanI GGYRCC 1 cut(s) 394
BarI GAAGNNNNNNTAC 2 cut(s) 1218, 1250
Bbv12I GWGCWC 1 cut(s) 595
BbvI GCAGC 4 cut(s) 8, 48, 229, 232
BccI CCATC 3 cut(s) 244, 269, 1272
BcgI CGANNNNNNTGC 2 cut(s) 647, 681
BciT130I CCWGG 1 cut(s) 767
BcnI CCSGG 1 cut(s) 151
BcoDI GTCTC 1 cut(s) 1250
BfaI CTAG 1 cut(s) 618
BfmI CTRYAG 2 cut(s) 1043, 1145
BisI GCNGC 5 cut(s) 22, 62, 177, 243, 246
BlsI GCNGC 5 cut(s) 23, 63, 178, 244, 247
Bme1390I CCNGG 2 cut(s) 151, 767
Bme18I GGWCC 2 cut(s) 136, 1101
BmeT110I CYCGRG 1 cut(s) 131
BmgT120I GGNCC 4 cut(s) 103, 136, 472, 1101
BmiI GGNNCC 4 cut(s) 396, 741, 846, 1102
BmrFI CCNGG 2 cut(s) 151, 767
BmsI GCATC 3 cut(s) 217, 613, 1209
BpuMI CCSGG 1 cut(s) 151
Bsa29I ATCGAT 1 cut(s) 657
BsaBI GATNNNNATC 1 cut(s) 966
BsaHI GRCGYC 1 cut(s) 155
BsaJI CCNNGG 3 cut(s) 99, 475, 839
BsaWI WCCGGW 3 cut(s) 430, 724, 958
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 150, 310, 1183
Bse118I RCCGGY 1 cut(s) 724
Bse1I ACTGG 2 cut(s) 191, 971
Bse3DI GCAATG 1 cut(s) 244
Bse8I GATNNNNATC 1 cut(s) 966
BseAI TCCGGA 1 cut(s) 958
BseBI CCWGG 1 cut(s) 767
BseCI ATCGAT 1 cut(s) 657
BseDI CCNNGG 3 cut(s) 99, 475, 839
BseGI GGATG 2 cut(s) 762, 967
BseJI GATNNNNATC 1 cut(s) 966
BseLI CCNNNNNNNGG 4 cut(s) 100, 150, 310, 1183
BseMI GCAATG 1 cut(s) 244
BseMII CTCAG 2 cut(s) 495, 597
BseNI ACTGG 2 cut(s) 191, 971
BseRI GAGGAG 2 cut(s) 752, 1256
BseXI GCAGC 4 cut(s) 8, 48, 229, 232
BsgI GTGCAG 1 cut(s) 7
Bsh1236I CGCG 2 cut(s) 77, 179
BshFI GGCC 3 cut(s) 93, 105, 474
BshNI GGYRCC 1 cut(s) 394
BshTI ACCGGT 1 cut(s) 724
BshVI ATCGAT 1 cut(s) 657
BsiHKAI GWGCWC 1 cut(s) 595
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 6 cut(s) 90, 150, 431, 588, 725, 959
BslFI GGGAC 1 cut(s) 166
BslI CCNNNNNNNGG 4 cut(s) 100, 150, 310, 1183
BsmAI GTCTC 1 cut(s) 1250
BsmFI GGGAC 1 cut(s) 166
BsnI GGCC 3 cut(s) 93, 105, 474
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 595
Bsp13I TCCGGA 1 cut(s) 958
Bsp143I GATC 3 cut(s) 127, 163, 1037
Bsp19I CCATGG 1 cut(s) 475
BspACI CCGC 4 cut(s) 5, 30, 77, 177
BspANI GGCC 3 cut(s) 93, 105, 474
BspCNI CTCAG 2 cut(s) 496, 598
BspDI ATCGAT 1 cut(s) 657
BspEI TCCGGA 1 cut(s) 958
BspFNI CGCG 2 cut(s) 77, 179
BspHI TCATGA 1 cut(s) 1015
BspLI GGNNCC 4 cut(s) 396, 741, 846, 1102
BspMAI CTGCAG 1 cut(s) 1149
BspPI GGATC 1 cut(s) 171
BspQI GCTCTTC 2 cut(s) 1022, 1049
BspT107I GGYRCC 1 cut(s) 394
BsrDI GCAATG 1 cut(s) 244
BsrFI RCCGGY 1 cut(s) 724
BsrI ACTGG 2 cut(s) 191, 971
BssAI RCCGGY 1 cut(s) 724
BssECI CCNNGG 3 cut(s) 99, 475, 839
BssMI GATC 3 cut(s) 127, 163, 1037
BssNI GRCGYC 1 cut(s) 155
BssT1I CCWWGG 3 cut(s) 99, 475, 839
Bst2UI CCWGG 1 cut(s) 767
Bst4CI ACNGT 5 cut(s) 391, 787, 899, 1001, 1076
Bst6I CTCTTC 5 cut(s) 1017, 1022, 1049, 1176, 1182
BstACI GRCGYC 1 cut(s) 155
BstAPI GCANNNNNTGC 1 cut(s) 214
BstC8I GCNNGC 1 cut(s) 452
BstDEI CTNAG 2 cut(s) 504, 606
BstDSI CCRYGG 1 cut(s) 475
BstF5I GGATG 2 cut(s) 762, 967
BstFNI CGCG 2 cut(s) 77, 179
BstKTI GATC 3 cut(s) 130, 166, 1040
BstMAI GTCTC 1 cut(s) 1250
BstMBI GATC 3 cut(s) 127, 163, 1037
BstMWI GCNNNNNNNGC 8 cut(s) 27, 67, 214, 254, 296, 383, 392, 623
BstNI CCWGG 1 cut(s) 767
BstNSI RCATGY 1 cut(s) 485
BstSCI CCNGG 2 cut(s) 149, 765
BstSFI CTRYAG 2 cut(s) 1043, 1145
BstUI CGCG 2 cut(s) 77, 179
BstV1I GCAGC 4 cut(s) 8, 48, 229, 232
Bsu15I ATCGAT 1 cut(s) 657
BsuRI GGCC 3 cut(s) 93, 105, 474
BsuTUI ATCGAT 1 cut(s) 657
BtgI CCRYGG 1 cut(s) 475
BtgZI GCGATG 1 cut(s) 263
BtsCI GGATG 2 cut(s) 762, 967
BtsIMutI CAGTG 4 cut(s) 317, 396, 978, 1081
Cac8I GCNNGC 1 cut(s) 452
CaiI CAGNNNCTG 1 cut(s) 211
CciI TCATGA 1 cut(s) 1015
Cfr10I RCCGGY 1 cut(s) 724
Cfr13I GGNCC 4 cut(s) 103, 136, 472, 1101
ClaI ATCGAT 1 cut(s) 657
CseI GACGC 1 cut(s) 163
Csp6I GTAC 2 cut(s) 13, 722
CspAI ACCGGT 1 cut(s) 724
CviAII CATG 9 cut(s) 109, 115, 476, 482, 530, 566, 628, 904, 1016
CviQI GTAC 2 cut(s) 13, 722
DdeI CTNAG 2 cut(s) 504, 606
DpnI GATC 3 cut(s) 129, 165, 1039
DpnII GATC 3 cut(s) 127, 163, 1037
DraIII CACNNNGTG 1 cut(s) 49
Eam1104I CTCTTC 5 cut(s) 1017, 1022, 1049, 1176, 1182
EarI CTCTTC 5 cut(s) 1017, 1022, 1049, 1176, 1182
Eco130I CCWWGG 3 cut(s) 99, 475, 839
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 2 cut(s) 136, 1101
Eco57I CTGAAG 1 cut(s) 1200
Eco88I CYCGRG 1 cut(s) 131
EcoO109I RGGNCCY 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 765
EcoRV GATATC 1 cut(s) 661
EcoT14I CCWWGG 3 cut(s) 99, 475, 839
EcoT22I ATGCAT 1 cut(s) 531
ErhI CCWWGG 3 cut(s) 99, 475, 839
FaeI CATG 9 cut(s) 112, 118, 479, 485, 533, 569, 631, 907, 1019
FaqI GGGAC 1 cut(s) 166
FatI CATG 9 cut(s) 108, 114, 475, 481, 529, 565, 627, 903, 1015
FauI CCCGC 1 cut(s) 70
FblI GTMKAC 1 cut(s) 990
Fnu4HI GCNGC 5 cut(s) 22, 62, 177, 243, 246
FokI GGATG 2 cut(s) 749, 974
Fsp4HI GCNGC 5 cut(s) 22, 62, 177, 243, 246
FspBI CTAG 1 cut(s) 618
GluI GCNGC 5 cut(s) 22, 62, 177, 243, 246
HaeIII GGCC 3 cut(s) 93, 105, 474
HapII CCGG 6 cut(s) 90, 150, 431, 588, 725, 959
HgaI GACGC 1 cut(s) 163
Hin1I GRCGYC 1 cut(s) 155
Hin1II CATG 9 cut(s) 112, 118, 479, 485, 533, 569, 631, 907, 1019
HincII GTYRAC 3 cut(s) 561, 783, 991
HindII GTYRAC 3 cut(s) 561, 783, 991
HindIII AAGCTT 1 cut(s) 671
HinfI GANTC 7 cut(s) 33, 322, 631, 691, 759, 835, 1277
HpaI GTTAAC 1 cut(s) 783
HpaII CCGG 6 cut(s) 90, 150, 431, 588, 725, 959
HphI GGTGA 6 cut(s) 88, 295, 325, 347, 799, 1013
Hpy166II GTNNAC 4 cut(s) 561, 783, 790, 991
Hpy188I TCNGA 2 cut(s) 607, 1138
Hpy188III TCNNGA 8 cut(s) 131, 272, 363, 848, 886, 959, 1016, 1104
Hpy8I GTNNAC 4 cut(s) 561, 783, 790, 991
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 2 cut(s) 154, 915
HpyCH4III ACNGT 5 cut(s) 391, 787, 899, 1001, 1076
HpyCH4IV ACGT 1 cut(s) 854
HpyF10VI GCNNNNNNNGC 8 cut(s) 27, 67, 214, 254, 296, 383, 392, 623
HpyF3I CTNAG 2 cut(s) 504, 606
HpySE526I ACGT 1 cut(s) 854
Hsp92I GRCGYC 1 cut(s) 155
Hsp92II CATG 9 cut(s) 112, 118, 479, 485, 533, 569, 631, 907, 1019
Kpn2I TCCGGA 1 cut(s) 958
KspAI GTTAAC 1 cut(s) 783
Kzo9I GATC 3 cut(s) 127, 163, 1037
LguI GCTCTTC 2 cut(s) 1022, 1049
LmnI GCTCC 4 cut(s) 590, 739, 940, 1179
Lsp1109I GCAGC 4 cut(s) 8, 48, 229, 232
LweI GCATC 3 cut(s) 217, 613, 1209
MaeI CTAG 1 cut(s) 618
MaeII ACGT 1 cut(s) 854
MaeIII GTNAC 5 cut(s) 283, 313, 820, 893, 899
MalI GATC 3 cut(s) 129, 165, 1039
MboI GATC 3 cut(s) 127, 163, 1037
MhlI GDGCHC 1 cut(s) 595
MluCI AATT 3 cut(s) 682, 775, 1084
MlyI GAGTC 3 cut(s) 625, 753, 1271
Mph1103I ATGCAT 1 cut(s) 531
MroI TCCGGA 1 cut(s) 958
MroXI GAANNNNTTC 1 cut(s) 881
MseI TTAA 1 cut(s) 782
MslI CAYNNNNRTG 5 cut(s) 113, 311, 480, 534, 1208
MspA1I CMGCKG 1 cut(s) 30
MspI CCGG 6 cut(s) 90, 150, 431, 588, 725, 959
MspR9I CCNGG 2 cut(s) 151, 767
MvaI CCWGG 1 cut(s) 767
MvnI CGCG 2 cut(s) 77, 179
MwoI GCNNNNNNNGC 8 cut(s) 27, 67, 214, 254, 296, 383, 392, 623
NciI CCSGG 1 cut(s) 151
NcoI CCATGG 1 cut(s) 475
NdeII GATC 3 cut(s) 127, 163, 1037
NlaIII CATG 9 cut(s) 112, 118, 479, 485, 533, 569, 631, 907, 1019
NlaIV GGNNCC 4 cut(s) 396, 741, 846, 1102
NmuCI GTSAC 5 cut(s) 283, 313, 820, 893, 899
NsiI ATGCAT 1 cut(s) 531
NspI RCATGY 1 cut(s) 485
PaeR7I CTCGAG 1 cut(s) 131
PagI TCATGA 1 cut(s) 1015
PciI ACATGT 1 cut(s) 481
PciSI GCTCTTC 2 cut(s) 1022, 1049
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PdmI GAANNNNTTC 1 cut(s) 881
PfeI GAWTC 4 cut(s) 33, 322, 691, 835
PfoI TCCNGGA 1 cut(s) 149
PinAI ACCGGT 1 cut(s) 724
PkrI GCNGC 5 cut(s) 23, 63, 178, 244, 247
PleI GAGTC 3 cut(s) 625, 753, 1271
PpsI GAGTC 3 cut(s) 625, 753, 1271
PpuMI RGGWCCY 1 cut(s) 136
PscI ACATGT 1 cut(s) 481
Psp1406I AACGTT 1 cut(s) 854
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 1 cut(s) 765
PspGI CCWGG 1 cut(s) 765
PspN4I GGNNCC 4 cut(s) 396, 741, 846, 1102
PspPI GGNCC 4 cut(s) 103, 136, 472, 1101
PspPPI RGGWCCY 1 cut(s) 136
PstI CTGCAG 1 cut(s) 1149
PstNI CAGNNNCTG 1 cut(s) 211
RsaI GTAC 2 cut(s) 14, 723
RsaNI GTAC 2 cut(s) 13, 722
RseI CAYNNNNRTG 5 cut(s) 113, 311, 480, 534, 1208
SalI GTCGAC 1 cut(s) 989
SapI GCTCTTC 2 cut(s) 1022, 1049
SaqAI TTAA 1 cut(s) 782
SatI GCNGC 5 cut(s) 22, 62, 177, 243, 246
Sau3AI GATC 3 cut(s) 127, 163, 1037
Sau96I GGNCC 4 cut(s) 103, 136, 472, 1101
SchI GAGTC 3 cut(s) 625, 753, 1271
ScrFI CCNGG 2 cut(s) 151, 767
SduI GDGCHC 1 cut(s) 595
SfaNI GCATC 3 cut(s) 217, 613, 1209
SfcI CTRYAG 2 cut(s) 1043, 1145
Sfr274I CTCGAG 1 cut(s) 131
SinI GGWCC 2 cut(s) 136, 1101
SlaI CTCGAG 1 cut(s) 131
SmiMI CAYNNNNRTG 5 cut(s) 113, 311, 480, 534, 1208
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
Sse9I AATT 3 cut(s) 682, 775, 1084
SsiI CCGC 4 cut(s) 5, 30, 77, 177
SspI AATATT 2 cut(s) 699, 751
SspMI CTAG 1 cut(s) 618
StyD4I CCNGG 2 cut(s) 149, 765
StyI CCWWGG 3 cut(s) 99, 475, 839
TaaI ACNGT 5 cut(s) 391, 787, 899, 1001, 1076
TaiI ACGT 1 cut(s) 857
TaqI TCGA 6 cut(s) 126, 132, 657, 990, 1224, 1251
TasI AATT 3 cut(s) 682, 775, 1084
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 4 cut(s) 33, 322, 691, 835
Tru1I TTAA 1 cut(s) 782
Tru9I TTAA 1 cut(s) 782
TscAI CASTG 4 cut(s) 324, 396, 978, 1081
TseFI GTSAC 5 cut(s) 283, 313, 820, 893, 899
TseI GCWGC 4 cut(s) 21, 61, 242, 245
Tsp45I GTSAC 5 cut(s) 283, 313, 820, 893, 899
TspDTI ATGAA 6 cut(s) 28, 585, 704, 816, 1004, 1077
TspGWI ACGGA 2 cut(s) 395, 953
TspRI CASTG 4 cut(s) 324, 396, 978, 1081
VpaK11BI GGWCC 2 cut(s) 136, 1101
XapI RAATTY 2 cut(s) 682, 775
XceI RCATGY 1 cut(s) 485
XhoI CTCGAG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 990
XmnI GAANNNNTTC 1 cut(s) 881
XspI CTAG 1 cut(s) 618
Zsp2I ATGCAT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.