Prupe.8G155800_v2.0.a1

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0007184
Physical Location & Seq
Forward (+)
16696700 .. 16701574
4875 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G155800.2

Sequence Viewer

Length: 1299 bp
ATGGCGGTGGAATACGAGTGTTGCAGTAGCGGGTTCTTCATTCACATAGCGCTGATCGTGCTGTTGGTGCTGTTCGCTGGGCTAATGTCTGGCCTCACCTTGGGGCTCATGTCCATGAGCGTCGTCGATCTCGAGGTCCTCGCAAAGTCCGGCACCCCCAAGGATCGCAAATACGCCGCGAAGATATTGCCTGTTGTAAAAAATCAGCATCTGCTGCTCTGCACCCTACTAATTTGCAATGCTGCTGCAATGGAGACACTTCCTATTTTCCTTGATGGTCTGGTGACAGCTTGGGGTGCTATCCTAATTTCGGTGACACTGATTCTTCTGTTTGGTGAGATTATACCACAATCTGTTTGTTCCCGATATGGTTTGGCGATAGGTGCAACAGTGGCTCCAGTTGTGCGCGTTCTTGTCTGGATCTGCATTCCTGTTGCATATCCAATTAGCAAGCTATTGGACTTTCTGCTGGGCCATGGACATGTAGCTCTATTTCGCAGAGCTGAGTTGAAAACACTTGTAGATATGCATGGTAATGAGGCTGGAAAAGGTGGAGAGTTGACACATGATGAGACAACAATTATTGCCGGAGCACTTGAGCTCTCCGAGAAAACAGCTAGTGATGCCATGACTCCTATAGCCGAAACTTTTGCAATTGATATCAATGCCAAGCTTGACAGGTTTATTATGAATTTGATGAACCTAATATTGGAGAAAGGACATAGCAGAGTTCCTGTTTATTATGAGGAGCCTACAAACATAATTGGACTCGTCCTGGTAAAAAATTTGTTGACGGTTAATCCAGAAGAGGAAGTGCCTGTAAAGAGTGTCACTGTACGCAGGATTCCAAGGGTTCCAGAAACGTTGCCGTTATATGACATATTGAATGAGTTTCAGAAAGGTCACAGCCACATGGCTATTGTTGTAAGACGATGCAATAAGAATGTGGATCAGACCAACGGTGATCCTGCTGACAATCCAGTGAAGGAAGTGAAAGTCGACATTGATGGTGAAAAGCCTCTTCAAGAGAAGATTTTGAAGAGCAAAAGATCACTGCAGAAGTGGAAGAGCTTTCCCAACACAAACAGTGCAAATAATTCATATAGGACCGGTTCCAGAAGCAAGAAATGGACAAAGGAGATATATTCAGACATCCTGCAGATTGATGGCGGTGAGCCACTCCCTAAGCTCCCTGAAGAGGAAGAGGCTGTTGGCATTATAACAATGGAAGATGTTATCGAAGAACTGTTACAGGAGGAGATTTTCGATGAGACTGATCATCAGTTTGACGACTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

433

Amino Acids

47.79

Weight (kDa)

5.22

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1220
AccB1I GGYRCC 1 cut(s) 152
AccI GTMKAC 1 cut(s) 999
AccII CGCG 2 cut(s) 179, 408
AciI CCGC 4 cut(s) 5, 30, 177, 1170
AclI AACGTT 1 cut(s) 863
AclWI GGATC 4 cut(s) 171, 428, 957, 959
AcsI RAATTY 2 cut(s) 691, 784
AcuI CTGAAG 1 cut(s) 1215
AfaI GTAC 1 cut(s) 837
AfeI AGCGCT 1 cut(s) 51
AfiI CCNNNNNNNGG 4 cut(s) 100, 310, 709, 1198
AflIII ACRYGT 1 cut(s) 481
AgeI ACCGGT 1 cut(s) 1109
AgsI TTSAA 4 cut(s) 511, 886, 1025, 1039
AjnI CCWGG 1 cut(s) 774
AjuI GAANNNNNNNTTGG 4 cut(s) 692, 724, 1194, 1226
AluBI AGCT 9 cut(s) 290, 454, 488, 503, 601, 617, 673, 1071, 1189
AluI AGCT 9 cut(s) 290, 454, 488, 503, 601, 617, 673, 1071, 1189
Alw21I GWGCWC 2 cut(s) 595, 603
Alw26I GTCTC 3 cut(s) 248, 566, 1265
AlwI GGATC 4 cut(s) 171, 428, 957, 959
AlwNI CAGNNNCTG 1 cut(s) 211
Ama87I CYCGRG 1 cut(s) 131
Aor51HI AGCGCT 1 cut(s) 51
AoxI GGCC 2 cut(s) 91, 472
ApeKI GCWGC 3 cut(s) 214, 242, 245
ApoI RAATTY 2 cut(s) 691, 784
AsiGI ACCGGT 1 cut(s) 1109
AspLEI GCGC 2 cut(s) 52, 408
AspS9I GGNCC 3 cut(s) 136, 472, 1107
AsuHPI GGTGA 7 cut(s) 88, 295, 325, 347, 974, 1022, 1184
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 2 cut(s) 136, 1107
BanI GGYRCC 1 cut(s) 152
BanII GRGCYC 2 cut(s) 108, 603
BarI GAAGNNNNNNTAC 2 cut(s) 1233, 1265
Bbv12I GWGCWC 2 cut(s) 595, 603
BbvI GCAGC 3 cut(s) 201, 229, 232
BccI CCATC 3 cut(s) 269, 1001, 1160
BceAI ACGGC 1 cut(s) 853
BcgI CGANNNNNNTGC 4 cut(s) 169, 203, 632, 666
BciT130I CCWGG 1 cut(s) 776
BclI TGATCA 1 cut(s) 1276
BcoDI GTCTC 3 cut(s) 248, 566, 1265
BfaI CTAG 1 cut(s) 618
BfmI CTRYAG 3 cut(s) 636, 1055, 1157
BfoI RGCGCY 1 cut(s) 53
BisI GCNGC 4 cut(s) 177, 215, 243, 246
BlsI GCNGC 4 cut(s) 178, 216, 244, 247
Bme1390I CCNGG 1 cut(s) 776
Bme18I GGWCC 2 cut(s) 136, 1107
BmeT110I CYCGRG 1 cut(s) 131
BmgT120I GGNCC 3 cut(s) 136, 472, 1107
BmiI GGNNCC 5 cut(s) 154, 396, 750, 855, 1114
BmrFI CCNGG 1 cut(s) 776
BmsI GCATC 3 cut(s) 217, 613, 923
BpmI CTGGAG 1 cut(s) 381
Bpu10I CCTNAGC 1 cut(s) 1185
BpuEI CTTGAG 1 cut(s) 617
BsaJI CCNNGG 4 cut(s) 99, 159, 475, 848
BsaWI WCCGGW 1 cut(s) 1109
BsaXI ACNNNNNCTCC 2 cut(s) 379, 409
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 310, 709, 1198
Bse118I RCCGGY 1 cut(s) 1109
Bse1I ACTGG 2 cut(s) 398, 980
Bse3DI GCAATG 2 cut(s) 244, 255
BseBI CCWGG 1 cut(s) 776
BseDI CCNNGG 4 cut(s) 99, 159, 475, 848
BseGI GGATG 1 cut(s) 1152
BseLI CCNNNNNNNGG 4 cut(s) 100, 310, 709, 1198
BseMI GCAATG 2 cut(s) 244, 255
BseMII CTCAG 1 cut(s) 495
BseNI ACTGG 2 cut(s) 398, 980
BseRI GAGGAG 2 cut(s) 761, 1271
BseXI GCAGC 3 cut(s) 201, 229, 232
BseYI CCCAGC 2 cut(s) 77, 469
BsgI GTGCAG 1 cut(s) 205
Bsh1236I CGCG 2 cut(s) 179, 408
BshFI GGCC 2 cut(s) 93, 474
BshNI GGYRCC 1 cut(s) 152
BshTI ACCGGT 1 cut(s) 1109
BsiHKAI GWGCWC 2 cut(s) 595, 603
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 3 cut(s) 150, 588, 1110
BslI CCNNNNNNNGG 4 cut(s) 100, 310, 709, 1198
BsmAI GTCTC 3 cut(s) 248, 566, 1265
BsmI GAATGC 1 cut(s) 426
BsnI GGCC 2 cut(s) 93, 474
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 3 cut(s) 108, 595, 603
Bsp143I GATC 8 cut(s) 54, 127, 163, 420, 949, 964, 1049, 1276
Bsp19I CCATGG 1 cut(s) 475
BspACI CCGC 4 cut(s) 5, 30, 177, 1170
BspANI GGCC 2 cut(s) 93, 474
BspCNI CTCAG 1 cut(s) 496
BspFNI CGCG 2 cut(s) 179, 408
BspLI GGNNCC 5 cut(s) 154, 396, 750, 855, 1114
BspMAI CTGCAG 2 cut(s) 1059, 1161
BspPI GGATC 4 cut(s) 171, 428, 957, 959
BspQI GCTCTTC 2 cut(s) 1034, 1061
BspT107I GGYRCC 1 cut(s) 152
BsrDI GCAATG 2 cut(s) 244, 255
BsrFI RCCGGY 1 cut(s) 1109
BsrI ACTGG 2 cut(s) 398, 980
BssAI RCCGGY 1 cut(s) 1109
BssECI CCNNGG 4 cut(s) 99, 159, 475, 848
BssMI GATC 8 cut(s) 54, 127, 163, 420, 949, 964, 1049, 1276
BssT1I CCWWGG 4 cut(s) 99, 159, 475, 848
Bst2UI CCWGG 1 cut(s) 776
Bst4CI ACNGT 6 cut(s) 391, 796, 835, 962, 1088, 1248
Bst6I CTCTTC 6 cut(s) 801, 1026, 1034, 1061, 1191, 1197
BstAPI GCANNNNNTGC 1 cut(s) 214
BstC8I GCNNGC 1 cut(s) 452
BstDEI CTNAG 2 cut(s) 504, 1185
BstDSI CCRYGG 1 cut(s) 475
BstF5I GGATG 1 cut(s) 1152
BstFNI CGCG 2 cut(s) 179, 408
BstH2I RGCGCY 1 cut(s) 53
BstHHI GCGC 2 cut(s) 52, 408
BstKTI GATC 8 cut(s) 57, 130, 166, 423, 952, 967, 1052, 1279
BstMAI GTCTC 3 cut(s) 248, 566, 1265
BstMBI GATC 8 cut(s) 54, 127, 163, 420, 949, 964, 1049, 1276
BstMWI GCNNNNNNNGC 7 cut(s) 58, 67, 214, 296, 383, 392, 623
BstNI CCWGG 1 cut(s) 776
BstNSI RCATGY 1 cut(s) 485
BstSCI CCNGG 1 cut(s) 774
BstSFI CTRYAG 3 cut(s) 636, 1055, 1157
BstUI CGCG 2 cut(s) 179, 408
BstV1I GCAGC 3 cut(s) 201, 229, 232
BstX2I RGATCY 1 cut(s) 420
BstYI RGATCY 1 cut(s) 420
BsuRI GGCC 2 cut(s) 93, 474
BtgI CCRYGG 1 cut(s) 475
BtsCI GGATG 1 cut(s) 1152
BtsI GCAGTG 1 cut(s) 1052
BtsIMutI CAGTG 6 cut(s) 317, 396, 831, 987, 1052, 1093
Cac8I GCNNGC 1 cut(s) 452
CaiI CAGNNNCTG 1 cut(s) 211
CfoI GCGC 2 cut(s) 52, 408
Cfr10I RCCGGY 1 cut(s) 1109
Cfr13I GGNCC 3 cut(s) 136, 472, 1107
CseI GACGC 1 cut(s) 109
Csp6I GTAC 1 cut(s) 836
CspAI ACCGGT 1 cut(s) 1109
CviAII CATG 8 cut(s) 109, 115, 476, 482, 530, 566, 628, 913
CviQI GTAC 1 cut(s) 836
DdeI CTNAG 2 cut(s) 504, 1185
DpnI GATC 8 cut(s) 56, 129, 165, 422, 951, 966, 1051, 1278
DpnII GATC 8 cut(s) 54, 127, 163, 420, 949, 964, 1049, 1276
Eam1104I CTCTTC 6 cut(s) 801, 1026, 1034, 1061, 1191, 1197
EarI CTCTTC 6 cut(s) 801, 1026, 1034, 1061, 1191, 1197
Ecl136II GAGCTC 1 cut(s) 601
Eco130I CCWWGG 4 cut(s) 99, 159, 475, 848
Eco24I GRGCYC 2 cut(s) 108, 603
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 2 cut(s) 136, 1107
Eco47III AGCGCT 1 cut(s) 51
Eco53kI GAGCTC 1 cut(s) 601
Eco57I CTGAAG 1 cut(s) 1215
Eco88I CYCGRG 1 cut(s) 131
EcoICRI GAGCTC 1 cut(s) 601
EcoO109I RGGNCCY 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 774
EcoRV GATATC 1 cut(s) 661
EcoT14I CCWWGG 4 cut(s) 99, 159, 475, 848
EcoT22I ATGCAT 1 cut(s) 531
EcoT38I GRGCYC 2 cut(s) 108, 603
ErhI CCWWGG 4 cut(s) 99, 159, 475, 848
FaeI CATG 8 cut(s) 112, 118, 479, 485, 533, 569, 631, 916
FatI CATG 8 cut(s) 108, 114, 475, 481, 529, 565, 627, 912
FauI CCCGC 1 cut(s) 23
FbaI TGATCA 1 cut(s) 1276
FblI GTMKAC 1 cut(s) 999
Fnu4HI GCNGC 4 cut(s) 177, 215, 243, 246
FokI GGATG 1 cut(s) 1139
FriOI GRGCYC 2 cut(s) 108, 603
Fsp4HI GCNGC 4 cut(s) 177, 215, 243, 246
FspBI CTAG 1 cut(s) 618
GlaI GCGC 2 cut(s) 51, 407
GluI GCNGC 4 cut(s) 177, 215, 243, 246
GsaI CCCAGC 2 cut(s) 81, 473
GsuI CTGGAG 1 cut(s) 381
HaeII RGCGCY 1 cut(s) 53
HaeIII GGCC 2 cut(s) 93, 474
HapII CCGG 3 cut(s) 150, 588, 1110
HgaI GACGC 1 cut(s) 109
HhaI GCGC 2 cut(s) 52, 408
Hin1II CATG 8 cut(s) 112, 118, 479, 485, 533, 569, 631, 916
Hin6I GCGC 2 cut(s) 50, 406
HinP1I GCGC 2 cut(s) 50, 406
HincII GTYRAC 3 cut(s) 561, 792, 1000
HindII GTYRAC 3 cut(s) 561, 792, 1000
HindIII AAGCTT 1 cut(s) 671
HinfI GANTC 5 cut(s) 322, 631, 768, 844, 1292
HpaII CCGG 3 cut(s) 150, 588, 1110
HphI GGTGA 7 cut(s) 88, 295, 325, 347, 974, 1022, 1184
Hpy166II GTNNAC 3 cut(s) 561, 792, 1000
Hpy188I TCNGA 4 cut(s) 607, 897, 954, 1150
Hpy188III TCNNGA 7 cut(s) 131, 363, 418, 803, 857, 1025, 1116
Hpy8I GTNNAC 3 cut(s) 561, 792, 1000
Hpy99I CGWCG 2 cut(s) 125, 128
HpyAV CCTTC 1 cut(s) 979
HpyCH4III ACNGT 6 cut(s) 391, 796, 835, 962, 1088, 1248
HpyCH4IV ACGT 1 cut(s) 863
HpyF10VI GCNNNNNNNGC 7 cut(s) 58, 67, 214, 296, 383, 392, 623
HpyF3I CTNAG 2 cut(s) 504, 1185
HpySE526I ACGT 1 cut(s) 863
Hsp92II CATG 8 cut(s) 112, 118, 479, 485, 533, 569, 631, 916
HspAI GCGC 2 cut(s) 50, 406
Ksp22I TGATCA 1 cut(s) 1276
Kzo9I GATC 8 cut(s) 54, 127, 163, 420, 949, 964, 1049, 1276
LguI GCTCTTC 2 cut(s) 1034, 1061
LmnI GCTCC 4 cut(s) 400, 590, 748, 1194
Lsp1109I GCAGC 3 cut(s) 201, 229, 232
LweI GCATC 3 cut(s) 217, 613, 923
MaeI CTAG 1 cut(s) 618
MaeII ACGT 1 cut(s) 863
MaeIII GTNAC 5 cut(s) 283, 313, 829, 902, 1248
MalI GATC 8 cut(s) 56, 129, 165, 422, 951, 966, 1051, 1278
MboI GATC 8 cut(s) 54, 127, 163, 420, 949, 964, 1049, 1276
MfeI CAATTG 1 cut(s) 654
MflI RGATCY 1 cut(s) 420
MhlI GDGCHC 3 cut(s) 108, 595, 603
MluCI AATT 9 cut(s) 231, 306, 444, 579, 654, 691, 762, 784, 1096
MlyI GAGTC 3 cut(s) 625, 762, 1286
Mph1103I ATGCAT 1 cut(s) 531
MseI TTAA 1 cut(s) 798
MslI CAYNNNNRTG 3 cut(s) 113, 480, 534
MspI CCGG 3 cut(s) 150, 588, 1110
MspR9I CCNGG 1 cut(s) 776
MunI CAATTG 1 cut(s) 654
Mva1269I GAATGC 1 cut(s) 426
MvaI CCWGG 1 cut(s) 776
MvnI CGCG 2 cut(s) 179, 408
MwoI GCNNNNNNNGC 7 cut(s) 58, 67, 214, 296, 383, 392, 623
NcoI CCATGG 1 cut(s) 475
NdeII GATC 8 cut(s) 54, 127, 163, 420, 949, 964, 1049, 1276
NlaIII CATG 8 cut(s) 112, 118, 479, 485, 533, 569, 631, 916
NlaIV GGNNCC 5 cut(s) 154, 396, 750, 855, 1114
NmuCI GTSAC 4 cut(s) 283, 313, 829, 902
NsiI ATGCAT 1 cut(s) 531
NspI RCATGY 1 cut(s) 485
PaeR7I CTCGAG 1 cut(s) 131
PciI ACATGT 1 cut(s) 481
PciSI GCTCTTC 2 cut(s) 1034, 1061
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 1 cut(s) 426
PfeI GAWTC 2 cut(s) 322, 844
PinAI ACCGGT 1 cut(s) 1109
PkrI GCNGC 4 cut(s) 178, 216, 244, 247
PleI GAGTC 3 cut(s) 625, 762, 1286
PpsI GAGTC 3 cut(s) 625, 762, 1286
PpuMI RGGWCCY 1 cut(s) 136
PscI ACATGT 1 cut(s) 481
PsiI TTATAA 1 cut(s) 1220
Psp124BI GAGCTC 1 cut(s) 603
Psp1406I AACGTT 1 cut(s) 863
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 1 cut(s) 774
PspFI CCCAGC 2 cut(s) 77, 469
PspGI CCWGG 1 cut(s) 774
PspN4I GGNNCC 5 cut(s) 154, 396, 750, 855, 1114
PspPI GGNCC 3 cut(s) 136, 472, 1107
PspPPI RGGWCCY 1 cut(s) 136
PstI CTGCAG 2 cut(s) 1059, 1161
PstNI CAGNNNCTG 1 cut(s) 211
PsuI RGATCY 1 cut(s) 420
RsaI GTAC 1 cut(s) 837
RsaNI GTAC 1 cut(s) 836
RseI CAYNNNNRTG 3 cut(s) 113, 480, 534
SacI GAGCTC 1 cut(s) 603
SalI GTCGAC 1 cut(s) 998
SapI GCTCTTC 2 cut(s) 1034, 1061
SaqAI TTAA 1 cut(s) 798
SatI GCNGC 4 cut(s) 177, 215, 243, 246
Sau3AI GATC 8 cut(s) 54, 127, 163, 420, 949, 964, 1049, 1276
Sau96I GGNCC 3 cut(s) 136, 472, 1107
SchI GAGTC 3 cut(s) 625, 762, 1286
ScrFI CCNGG 1 cut(s) 776
SduI GDGCHC 3 cut(s) 108, 595, 603
SfaNI GCATC 3 cut(s) 217, 613, 923
SfcI CTRYAG 3 cut(s) 636, 1055, 1157
Sfr274I CTCGAG 1 cut(s) 131
SinI GGWCC 2 cut(s) 136, 1107
SlaI CTCGAG 1 cut(s) 131
SmiMI CAYNNNNRTG 3 cut(s) 113, 480, 534
SmlI CTYRAG 2 cut(s) 131, 596
SmoI CTYRAG 2 cut(s) 131, 596
Sse9I AATT 9 cut(s) 231, 306, 444, 579, 654, 691, 762, 784, 1096
SsiI CCGC 4 cut(s) 5, 30, 177, 1170
SspI AATATT 1 cut(s) 708
SspMI CTAG 1 cut(s) 618
SstI GAGCTC 1 cut(s) 603
StyD4I CCNGG 1 cut(s) 774
StyI CCWWGG 4 cut(s) 99, 159, 475, 848
TaaI ACNGT 6 cut(s) 391, 796, 835, 962, 1088, 1248
TaiI ACGT 1 cut(s) 866
TaqI TCGA 5 cut(s) 126, 132, 999, 1239, 1266
TasI AATT 9 cut(s) 231, 306, 444, 579, 654, 691, 762, 784, 1096
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 2 cut(s) 322, 844
Tru1I TTAA 1 cut(s) 798
Tru9I TTAA 1 cut(s) 798
TscAI CASTG 6 cut(s) 324, 396, 838, 987, 1059, 1093
TseFI GTSAC 4 cut(s) 283, 313, 829, 902
TseI GCWGC 3 cut(s) 214, 242, 245
Tsp45I GTSAC 4 cut(s) 283, 313, 829, 902
TspDTI ATGAA 4 cut(s) 28, 704, 713, 1089
TspRI CASTG 6 cut(s) 324, 396, 838, 987, 1059, 1093
VpaK11BI GGWCC 2 cut(s) 136, 1107
XapI RAATTY 2 cut(s) 691, 784
XceI RCATGY 1 cut(s) 485
XhoI CTCGAG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 999
XspI CTAG 1 cut(s) 618
Zsp2I ATGCAT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.