pycom10g09870

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007184
Physical Location & Seq
Forward (+)
13044731 .. 13048512
3782 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g09870.8

Sequence Viewer

Length: 1242 bp
ATGAAAAAGGTGACCTTTAATGGCGGTGGAGTACGAGTGTTGCACCAGCGGATTCTTCATTCACATAGTGGTCATCGTGCTGCTGGTGATGTTCGCGGGGCTAATGTCCGGCCTCACCTTGGGCCTCATGTCCATGAGCCTCGTCGATCGATCGAGGTCCTCGCCAAGTCCGGGACGCCGAAGGATCGCAAACACGCCGCCAAGATATTACCAGTTGTCAAAAAACAGCATCTGTTGCTTTGCACTCTGCTGATTTGCAATGCTGCTGCGATGGAGGCACTCCCCATTTTTCTTGATGGTTTGGTAACAGCTTGGGGTGCAATCCTCATTTCGGTGACACTGATTCTTCTGTTTGGCGAGATTATACCACAATCTGTTTGTTCCCGATATGGTTTGGCTATTGGTGCAACAGTGGCACCATTTGTCCGTGTTCTTGTTTGGGTCTGCTTTCCGGTTGCATATCCTATTAGCAAGCTATTAGACTATCTGTTGGGCCATGGACATGTAGCTCTATTTCGCAGAGCTGAGTTGAAAACGCTTGTAGATATGCATGGAGCACTTGAACTCTCTGAGAAAATAGCTAGAGATGCCATGACTCCCATAGCTGAAACTTTTGCAATCGATATCAATGACAAGCTTGACAGGAATTTGATGAATATAATATTGGAGAAAGGGCATAGCAGAGTACCGGTTTATTATGAGGAGCCTATGAATATTATTGGACTCATTCTGGTAAAAAATTTGTTAACGGTGAACCCAGAAGATGAAGTGCCCGTAAAGAATGTCACTATACGCAAGATTCCAAGGGTTCCAGAAACGTTGCCCTTATATGACATATTGAACGAGTTCCAGAAAGGTCACAGTCACATGGCTATTGTTGTTAGAAGGTGCAATAAGAACGCGGAGCAGACTAATGGCAATCCAGATGACATGAAAGAAGTGAAAGTCGATATTGACGGTGAAAAGCCTCTTCATGAGAAGTTGAAGAGCAAAAGATCACTACAGAAGTGGAAGAGCTTTACAAATACAAACAGTGCAAATAATTCATACAGGAGCGGGTCCAGAAGCAAGAAATGGACAAAGGAGATATATTCAGACATCCTGCAGATTGATAGTGAGCCACTCCCCAAGCTCCCTGAAGAGGAAGAGGCCGTTGGCATCATAACAATGGAAGATGTTATCGAAGAACTTTTACAGGAGGAGATTTTCGATGAGACTGACCATCAGTTTGAGGACTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

414

Amino Acids

46.38

Weight (kDa)

7.0

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 415
AccBSI CCGCTC 1 cut(s) 1056
AccII CGCG 2 cut(s) 96, 902
AciI CCGC 6 cut(s) 24, 49, 96, 198, 902, 1056
AclI AACGTT 1 cut(s) 818
AclWI GGATC 1 cut(s) 192
AcsI RAATTY 2 cut(s) 646, 739
AcuI CTGAAG 1 cut(s) 1158
AcyI GRCGYC 1 cut(s) 176
AdeI CACNNNGTG 1 cut(s) 68
AfaI GTAC 2 cut(s) 33, 687
AfiI CCNNNNNNNGG 4 cut(s) 119, 171, 331, 1141
AflIII ACRYGT 1 cut(s) 502
AgeI ACCGGT 1 cut(s) 688
AgsI TTSAA 4 cut(s) 532, 563, 841, 985
AjuI GAANNNNNNNTTGG 4 cut(s) 647, 679, 1137, 1169
AluBI AGCT 9 cut(s) 311, 475, 509, 524, 581, 605, 637, 1017, 1132
AluI AGCT 9 cut(s) 311, 475, 509, 524, 581, 605, 637, 1017, 1132
Alw21I GWGCWC 1 cut(s) 559
Alw26I GTCTC 1 cut(s) 1208
AlwI GGATC 1 cut(s) 192
AlwNI CAGNNNCTG 1 cut(s) 232
AoxI GGCC 4 cut(s) 110, 122, 493, 1149
ApeKI GCWGC 3 cut(s) 80, 263, 266
ApoI RAATTY 2 cut(s) 646, 739
ArsI GACNNNNNNTTYG 1 cut(s) 1211
AsiGI ACCGGT 1 cut(s) 688
Asp700I GAANNNNTTC 1 cut(s) 845
AspS9I GGNCC 4 cut(s) 122, 157, 493, 1059
AsuC2I CCSGG 1 cut(s) 172
AsuHPI GGTGA 6 cut(s) 22, 98, 107, 346, 763, 971
AvaII GGWCC 2 cut(s) 157, 1059
BaeGI GKGCMC 1 cut(s) 774
BanI GGYRCC 1 cut(s) 415
BarI GAAGNNNNNNTAC 2 cut(s) 1176, 1208
Bbv12I GWGCWC 1 cut(s) 559
BbvI GCAGC 3 cut(s) 67, 250, 253
BccI CCATC 3 cut(s) 265, 290, 1230
BceAI ACGGC 1 cut(s) 1136
BcnI CCSGG 1 cut(s) 172
BcoDI GTCTC 1 cut(s) 1208
BfaI CTAG 1 cut(s) 582
BfmI CTRYAG 2 cut(s) 1001, 1103
BisI GCNGC 4 cut(s) 81, 198, 264, 267
BlsI GCNGC 4 cut(s) 82, 199, 265, 268
Bme1390I CCNGG 1 cut(s) 172
Bme18I GGWCC 2 cut(s) 157, 1059
BmgT120I GGNCC 4 cut(s) 122, 157, 493, 1059
BmiI GGNNCC 4 cut(s) 417, 705, 810, 1060
BmrFI CCNGG 1 cut(s) 172
BmsI GCATC 3 cut(s) 238, 577, 1167
BpuMI CCSGG 1 cut(s) 172
Bsa29I ATCGAT 2 cut(s) 149, 621
BsaHI GRCGYC 1 cut(s) 176
BsaJI CCNNGG 3 cut(s) 118, 496, 803
BsaWI WCCGGW 2 cut(s) 451, 688
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bsc4I CCNNNNNNNGG 4 cut(s) 119, 171, 331, 1141
Bse118I RCCGGY 1 cut(s) 688
Bse1I ACTGG 1 cut(s) 212
Bse3DI GCAATG 1 cut(s) 265
BseCI ATCGAT 2 cut(s) 149, 621
BseDI CCNNGG 3 cut(s) 118, 496, 803
BseGI GGATG 1 cut(s) 1098
BseLI CCNNNNNNNGG 4 cut(s) 119, 171, 331, 1141
BseMI GCAATG 1 cut(s) 265
BseMII CTCAG 2 cut(s) 516, 561
BseNI ACTGG 1 cut(s) 212
BseRI GAGGAG 2 cut(s) 716, 1214
BseSI GKGCMC 1 cut(s) 774
BseXI GCAGC 3 cut(s) 67, 250, 253
Bsh1236I CGCG 2 cut(s) 96, 902
Bsh1285I CGRYCG 2 cut(s) 149, 153
BshFI GGCC 4 cut(s) 112, 124, 495, 1151
BshNI GGYRCC 1 cut(s) 415
BshTI ACCGGT 1 cut(s) 688
BshVI ATCGAT 2 cut(s) 149, 621
BsiEI CGRYCG 2 cut(s) 149, 153
BsiHKAI GWGCWC 1 cut(s) 559
BsiSI CCGG 4 cut(s) 109, 171, 452, 689
BslFI GGGAC 1 cut(s) 187
BslI CCNNNNNNNGG 4 cut(s) 119, 171, 331, 1141
BsmAI GTCTC 1 cut(s) 1208
BsmFI GGGAC 1 cut(s) 187
BsnI GGCC 4 cut(s) 112, 124, 495, 1151
Bsp1286I GDGCHC 2 cut(s) 559, 774
Bsp143I GATC 4 cut(s) 146, 150, 184, 995
Bsp19I CCATGG 1 cut(s) 496
BspACI CCGC 6 cut(s) 24, 49, 96, 198, 902, 1056
BspANI GGCC 4 cut(s) 112, 124, 495, 1151
BspCNI CTCAG 2 cut(s) 517, 562
BspDI ATCGAT 2 cut(s) 149, 621
BspFNI CGCG 2 cut(s) 96, 902
BspHI TCATGA 1 cut(s) 973
BspLI GGNNCC 4 cut(s) 417, 705, 810, 1060
BspMAI CTGCAG 1 cut(s) 1107
BspPI GGATC 1 cut(s) 192
BspQI GCTCTTC 2 cut(s) 980, 1007
BspT107I GGYRCC 1 cut(s) 415
BsrBI CCGCTC 1 cut(s) 1056
BsrDI GCAATG 1 cut(s) 265
BsrFI RCCGGY 1 cut(s) 688
BsrI ACTGG 1 cut(s) 212
BssAI RCCGGY 1 cut(s) 688
BssECI CCNNGG 3 cut(s) 118, 496, 803
BssMI GATC 4 cut(s) 146, 150, 184, 995
BssNI GRCGYC 1 cut(s) 176
BssT1I CCWWGG 3 cut(s) 118, 496, 803
Bst4CI ACNGT 5 cut(s) 412, 751, 863, 959, 1034
Bst6I CTCTTC 5 cut(s) 975, 980, 1007, 1134, 1140
BstACI GRCGYC 1 cut(s) 176
BstAPI GCANNNNNTGC 1 cut(s) 235
BstC8I GCNNGC 1 cut(s) 473
BstDEI CTNAG 2 cut(s) 525, 570
BstDSI CCRYGG 1 cut(s) 496
BstEII GGTNACC 1 cut(s) 10
BstF5I GGATG 1 cut(s) 1098
BstFNI CGCG 2 cut(s) 96, 902
BstKTI GATC 4 cut(s) 149, 153, 187, 998
BstMAI GTCTC 1 cut(s) 1208
BstMBI GATC 4 cut(s) 146, 150, 184, 995
BstMCI CGRYCG 2 cut(s) 149, 153
BstMWI GCNNNNNNNGC 6 cut(s) 235, 275, 317, 404, 413, 587
BstNSI RCATGY 1 cut(s) 506
BstPI GGTNACC 1 cut(s) 10
BstSCI CCNGG 1 cut(s) 170
BstSFI CTRYAG 2 cut(s) 1001, 1103
BstSLI GKGCMC 1 cut(s) 774
BstUI CGCG 2 cut(s) 96, 902
BstV1I GCAGC 3 cut(s) 67, 250, 253
Bsu15I ATCGAT 2 cut(s) 149, 621
BsuRI GGCC 4 cut(s) 112, 124, 495, 1151
BsuTUI ATCGAT 2 cut(s) 149, 621
BtgI CCRYGG 1 cut(s) 496
BtgZI GCGATG 1 cut(s) 284
BtsCI GGATG 1 cut(s) 1098
BtsIMutI CAGTG 3 cut(s) 338, 417, 1039
Cac8I GCNNGC 1 cut(s) 473
CaiI CAGNNNCTG 1 cut(s) 232
CciI TCATGA 1 cut(s) 973
Cfr10I RCCGGY 1 cut(s) 688
Cfr13I GGNCC 4 cut(s) 122, 157, 493, 1059
ClaI ATCGAT 2 cut(s) 149, 621
CseI GACGC 1 cut(s) 184
Csp6I GTAC 2 cut(s) 32, 686
CspAI ACCGGT 1 cut(s) 688
CviAII CATG 9 cut(s) 128, 134, 497, 503, 551, 592, 868, 931, 974
CviQI GTAC 2 cut(s) 32, 686
DdeI CTNAG 2 cut(s) 525, 570
DpnI GATC 4 cut(s) 148, 152, 186, 997
DpnII GATC 4 cut(s) 146, 150, 184, 995
DraIII CACNNNGTG 1 cut(s) 68
Eam1104I CTCTTC 5 cut(s) 975, 980, 1007, 1134, 1140
EarI CTCTTC 5 cut(s) 975, 980, 1007, 1134, 1140
Eco130I CCWWGG 3 cut(s) 118, 496, 803
Eco32I GATATC 1 cut(s) 625
Eco47I GGWCC 2 cut(s) 157, 1059
Eco57I CTGAAG 1 cut(s) 1158
Eco91I GGTNACC 1 cut(s) 10
EcoO109I RGGNCCY 1 cut(s) 157
EcoO65I GGTNACC 1 cut(s) 10
EcoRV GATATC 1 cut(s) 625
EcoT14I CCWWGG 3 cut(s) 118, 496, 803
EcoT22I ATGCAT 1 cut(s) 552
ErhI CCWWGG 3 cut(s) 118, 496, 803
FaeI CATG 9 cut(s) 131, 137, 500, 506, 554, 595, 871, 934, 977
FalI AAGNNNNNCTT 1 cut(s) 31
FaqI GGGAC 1 cut(s) 187
FatI CATG 9 cut(s) 127, 133, 496, 502, 550, 591, 867, 930, 973
FauI CCCGC 2 cut(s) 89, 1049
Fnu4HI GCNGC 4 cut(s) 81, 198, 264, 267
FokI GGATG 1 cut(s) 1085
Fsp4HI GCNGC 4 cut(s) 81, 198, 264, 267
FspBI CTAG 1 cut(s) 582
GluI GCNGC 4 cut(s) 81, 198, 264, 267
HaeIII GGCC 4 cut(s) 112, 124, 495, 1151
HapII CCGG 4 cut(s) 109, 171, 452, 689
HgaI GACGC 1 cut(s) 184
Hin1I GRCGYC 1 cut(s) 176
Hin1II CATG 9 cut(s) 131, 137, 500, 506, 554, 595, 871, 934, 977
HincII GTYRAC 1 cut(s) 747
HindII GTYRAC 1 cut(s) 747
HindIII AAGCTT 1 cut(s) 635
HinfI GANTC 6 cut(s) 52, 343, 595, 723, 799, 1235
HpaI GTTAAC 1 cut(s) 747
HpaII CCGG 4 cut(s) 109, 171, 452, 689
HphI GGTGA 6 cut(s) 22, 98, 107, 346, 763, 971
Hpy166II GTNNAC 2 cut(s) 747, 754
Hpy188I TCNGA 2 cut(s) 571, 1096
Hpy188III TCNNGA 7 cut(s) 293, 384, 812, 850, 923, 974, 1062
Hpy8I GTNNAC 2 cut(s) 747, 754
Hpy99I CGWCG 1 cut(s) 147
HpyAV CCTTC 2 cut(s) 175, 879
HpyCH4III ACNGT 5 cut(s) 412, 751, 863, 959, 1034
HpyCH4IV ACGT 1 cut(s) 818
HpyF10VI GCNNNNNNNGC 6 cut(s) 235, 275, 317, 404, 413, 587
HpyF3I CTNAG 2 cut(s) 525, 570
HpySE526I ACGT 1 cut(s) 818
Hsp92I GRCGYC 1 cut(s) 176
Hsp92II CATG 9 cut(s) 131, 137, 500, 506, 554, 595, 871, 934, 977
KspAI GTTAAC 1 cut(s) 747
Kzo9I GATC 4 cut(s) 146, 150, 184, 995
LguI GCTCTTC 2 cut(s) 980, 1007
LmnI GCTCC 5 cut(s) 554, 703, 904, 1053, 1137
Lsp1109I GCAGC 3 cut(s) 67, 250, 253
LweI GCATC 3 cut(s) 238, 577, 1167
MaeI CTAG 1 cut(s) 582
MaeII ACGT 1 cut(s) 818
MaeIII GTNAC 6 cut(s) 10, 304, 334, 784, 857, 863
MalI GATC 4 cut(s) 148, 152, 186, 997
MbiI CCGCTC 1 cut(s) 1056
MboI GATC 4 cut(s) 146, 150, 184, 995
MhlI GDGCHC 2 cut(s) 559, 774
MluCI AATT 3 cut(s) 646, 739, 1042
MlyI GAGTC 3 cut(s) 589, 717, 1229
Mph1103I ATGCAT 1 cut(s) 552
MroXI GAANNNNTTC 1 cut(s) 845
MseI TTAA 2 cut(s) 18, 746
MslI CAYNNNNRTG 4 cut(s) 132, 332, 501, 1166
MspA1I CMGCKG 1 cut(s) 49
MspI CCGG 4 cut(s) 109, 171, 452, 689
MspR9I CCNGG 1 cut(s) 172
MvnI CGCG 2 cut(s) 96, 902
MwoI GCNNNNNNNGC 6 cut(s) 235, 275, 317, 404, 413, 587
NciI CCSGG 1 cut(s) 172
NcoI CCATGG 1 cut(s) 496
NdeII GATC 4 cut(s) 146, 150, 184, 995
NlaIII CATG 9 cut(s) 131, 137, 500, 506, 554, 595, 871, 934, 977
NlaIV GGNNCC 4 cut(s) 417, 705, 810, 1060
NmuCI GTSAC 5 cut(s) 10, 334, 784, 857, 863
NsiI ATGCAT 1 cut(s) 552
NspI RCATGY 1 cut(s) 506
PagI TCATGA 1 cut(s) 973
PciI ACATGT 1 cut(s) 502
PciSI GCTCTTC 2 cut(s) 980, 1007
PdmI GAANNNNTTC 1 cut(s) 845
PfeI GAWTC 3 cut(s) 52, 343, 799
PfoI TCCNGGA 1 cut(s) 170
PinAI ACCGGT 1 cut(s) 688
PkrI GCNGC 4 cut(s) 82, 199, 265, 268
Ple19I CGATCG 2 cut(s) 149, 153
PleI GAGTC 3 cut(s) 589, 717, 1229
PpsI GAGTC 3 cut(s) 589, 717, 1229
PpuMI RGGWCCY 1 cut(s) 157
PscI ACATGT 1 cut(s) 502
Psp1406I AACGTT 1 cut(s) 818
Psp5II RGGWCCY 1 cut(s) 157
PspEI GGTNACC 1 cut(s) 10
PspN4I GGNNCC 4 cut(s) 417, 705, 810, 1060
PspPI GGNCC 4 cut(s) 122, 157, 493, 1059
PspPPI RGGWCCY 1 cut(s) 157
PstI CTGCAG 1 cut(s) 1107
PstNI CAGNNNCTG 1 cut(s) 232
PvuI CGATCG 2 cut(s) 149, 153
RsaI GTAC 2 cut(s) 33, 687
RsaNI GTAC 2 cut(s) 32, 686
RseI CAYNNNNRTG 4 cut(s) 132, 332, 501, 1166
SapI GCTCTTC 2 cut(s) 980, 1007
SaqAI TTAA 2 cut(s) 18, 746
SatI GCNGC 4 cut(s) 81, 198, 264, 267
Sau3AI GATC 4 cut(s) 146, 150, 184, 995
Sau96I GGNCC 4 cut(s) 122, 157, 493, 1059
SchI GAGTC 3 cut(s) 589, 717, 1229
ScrFI CCNGG 1 cut(s) 172
SduI GDGCHC 2 cut(s) 559, 774
SfaNI GCATC 3 cut(s) 238, 577, 1167
SfcI CTRYAG 2 cut(s) 1001, 1103
SinI GGWCC 2 cut(s) 157, 1059
SmiMI CAYNNNNRTG 4 cut(s) 132, 332, 501, 1166
Sse9I AATT 3 cut(s) 646, 739, 1042
SsiI CCGC 6 cut(s) 24, 49, 96, 198, 902, 1056
SspI AATATT 2 cut(s) 663, 715
SspMI CTAG 1 cut(s) 582
StyD4I CCNGG 1 cut(s) 170
StyI CCWWGG 3 cut(s) 118, 496, 803
TaaI ACNGT 5 cut(s) 412, 751, 863, 959, 1034
TaiI ACGT 1 cut(s) 821
TaqI TCGA 7 cut(s) 145, 149, 153, 621, 948, 1182, 1209
TasI AATT 3 cut(s) 646, 739, 1042
TauI GCSGC 1 cut(s) 200
TfiI GAWTC 3 cut(s) 52, 343, 799
Tru1I TTAA 2 cut(s) 18, 746
Tru9I TTAA 2 cut(s) 18, 746
TscAI CASTG 3 cut(s) 345, 417, 1039
TseFI GTSAC 5 cut(s) 10, 334, 784, 857, 863
TseI GCWGC 3 cut(s) 80, 263, 266
Tsp45I GTSAC 5 cut(s) 10, 334, 784, 857, 863
TspDTI ATGAA 8 cut(s) 17, 47, 668, 725, 780, 947, 962, 1035
TspGWI ACGGA 1 cut(s) 416
TspRI CASTG 3 cut(s) 345, 417, 1039
VpaK11BI GGWCC 2 cut(s) 157, 1059
XapI RAATTY 2 cut(s) 646, 739
XceI RCATGY 1 cut(s) 506
XmnI GAANNNNTTC 1 cut(s) 845
XspI CTAG 1 cut(s) 582
Zsp2I ATGCAT 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.