Rorug05G0523900

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007184
Physical Location & Seq
Forward (+)
71005226 .. 71006956
1731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0523900.1

Sequence Viewer

Length: 1305 bp
ATGACGAATGCAGGCAAAAGCAATCTGCTGCTGCTGTTATGCACTTGTCTTTTTTCAGGGTTTGAAGTAATTGCACTATCCTATGACTACACAGCCAGCATTGAGTGTTTGGAAAACCCTCACAAACCCCAATATAGTGGAGGAATCATTGTAAACCCTGAACTAAATCACGGCTTGAGAGGGTGGTCTACTTTCGGCGATGCAAAACTCCAACATAGAGAATCACAAGCAGGCAACAAATTCGCTGTGGCTCGTAATAGAAACCAACCACATGCTGGTATTTCACAAAAGACTTATTTGCAAAGAAACAAGCTTTATACATTCTCCGCTTGGATACAAGTGAGTGGTGGCAGAGGTGTTCCAGTAACAGTTATTTTCAAGACAAGTGCTGGATCATTCATTCATGCTGGTGCTATTGTCGCGGAATCTAATTGCTGGTCCATGCTCAAGGGTGGCCTAACTGTGAATGCATCAAGCCCAGCTGAGCTTTATTTTGAGAGTAAGAACACATCGGTTGAGATTTGGGTTGATAGCGTCTCATTACAACCATTCAACAAAAAGGAATGGAAGTCTCATTATGATCAAAGCATTGAGAAGAACCGAAAGAGCATCGTGAGAATCCAAGCAGTTGATGCAAAAGGAAACCCTCTACGGAATGCAACAATATCCATCCAACAAAAGGCTCCCAGATTCCCTTTCGGATGCGCTATGAACAAGAACATTCTCACCAATACTGCTTACCAAAACTGGTTCACTTCGAGGTTTGGAGTCACAACTTTTGAAGACGAAATGAAATGGTACAGCACAGAACCTTCACAGGGACAAGAGGACATACTACTCACCATTTTATACAATTGGGCTACTAAAGCTGATGGAGCAACCACCTTGTTCATGATTGACTACAATACCATTGAGGACAGCAAAGATGGAGCCTCCACCCCGGCTAAGTACCTTCAAAAGCTCAGAGATATACAAGGCTTTCCCGGTAACAACAACTTAAGGATGGCAATAGGACTTGAGTCTCATTTCAAGACTCCTAACATTCCTTACATAAGATCTTCTATTGATACTCTGGCTGCGCTGCAAACCTACCAATCTGGATTACAGAATTGGATGTGGAAAGCGGTCCCAATCAAGCATGATACTAATGGTGTCTTCGAGGCCTGCCTTCCCCATGGAGATTATGAAGTGAAAATCACTGACCTGGAATCTGTGAAAGTTTCTACCGGTATGGTTCAGGGATTGAATGTGGCAGCAAGTTCCACTGCATACCAACAACCATTGCTGGTCCAACTTGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

434

Amino Acids

48.18

Weight (kDa)

8.7

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM_4_9 PF02018 49 - 170 1.8e-08 Carbohydrate binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 275
AccI GTMKAC 1 cut(s) 188
AccII CGCG 1 cut(s) 422
AciI CCGC 3 cut(s) 327, 422, 1124
AclWI GGATC 1 cut(s) 400
AcsI RAATTY 1 cut(s) 239
AfaI GTAC 2 cut(s) 800, 950
AfiI CCNNNNNNNGG 3 cut(s) 275, 679, 818
AflII CTTAAG 1 cut(s) 997
AgeI ACCGGT 1 cut(s) 1226
AgsI TTSAA 7 cut(s) 65, 379, 553, 782, 956, 1030, 1246
AjnI CCWGG 1 cut(s) 1203
AluBI AGCT 5 cut(s) 313, 482, 487, 869, 961
AluI AGCT 5 cut(s) 313, 482, 487, 869, 961
Alw26I GTCTC 3 cut(s) 541, 576, 1026
AlwI GGATC 1 cut(s) 400
AoxI GGCC 2 cut(s) 454, 1161
ApeKI GCWGC 6 cut(s) 28, 31, 1076, 1081, 1253, 1298
ApoI RAATTY 1 cut(s) 239
AsiGI ACCGGT 1 cut(s) 1226
AspLEI GCGC 2 cut(s) 707, 1081
AspS9I GGNCC 3 cut(s) 438, 1126, 1288
AsuC2I CCSGG 2 cut(s) 941, 984
AsuHPI GGTGA 2 cut(s) 718, 832
AvaII GGWCC 3 cut(s) 438, 1126, 1288
BbsI GAAGAC 2 cut(s) 789, 1147
BbvI GCAGC 6 cut(s) 15, 18, 1063, 1068, 1265, 1285
BccI CCATC 4 cut(s) 677, 866, 920, 997
BceAI ACGGC 1 cut(s) 187
BcgI CGANNNNNNTGC 2 cut(s) 223, 257
BciT130I CCWGG 1 cut(s) 1205
BciVI GTATCC 1 cut(s) 327
BclI TGATCA 1 cut(s) 580
BcnI CCSGG 2 cut(s) 941, 984
BcoDI GTCTC 3 cut(s) 541, 576, 1026
BfrI CTTAAG 1 cut(s) 997
BfuI GTATCC 1 cut(s) 327
BglII AGATCT 1 cut(s) 1055
BisI GCNGC 6 cut(s) 29, 32, 1077, 1082, 1254, 1299
BlpI GCTNAGC 1 cut(s) 483
BlsI GCNGC 6 cut(s) 30, 33, 1078, 1083, 1255, 1300
Bme1390I CCNGG 3 cut(s) 941, 984, 1205
Bme18I GGWCC 3 cut(s) 438, 1126, 1288
BmgT120I GGNCC 3 cut(s) 438, 1126, 1288
BmiI GGNNCC 3 cut(s) 684, 931, 1128
BmrFI CCNGG 3 cut(s) 941, 984, 1205
BmsI GCATC 5 cut(s) 190, 479, 618, 622, 692
BoxI GACNNNNGTC 1 cut(s) 1018
BpiI GAAGAC 2 cut(s) 789, 1147
Bpu1102I GCTNAGC 1 cut(s) 483
BpuEI CTTGAG 3 cut(s) 196, 431, 1037
BpuMI CCSGG 2 cut(s) 941, 984
BsaJI CCNNGG 2 cut(s) 939, 1174
BsaWI WCCGGW 1 cut(s) 1226
Bsc4I CCNNNNNNNGG 3 cut(s) 275, 679, 818
Bse118I RCCGGY 1 cut(s) 1226
Bse1I ACTGG 2 cut(s) 362, 752
Bse3DI GCAATG 1 cut(s) 1280
BseBI CCWGG 1 cut(s) 1205
BseDI CCNNGG 2 cut(s) 939, 1174
BseGI GGATG 4 cut(s) 669, 707, 1008, 1119
BseLI CCNNNNNNNGG 3 cut(s) 275, 679, 818
BseMI GCAATG 1 cut(s) 1280
BseMII CTCAG 2 cut(s) 474, 976
BseNI ACTGG 2 cut(s) 362, 752
BseXI GCAGC 6 cut(s) 15, 18, 1063, 1068, 1265, 1285
BseYI CCCAGC 1 cut(s) 478
Bsh1236I CGCG 1 cut(s) 422
BshFI GGCC 2 cut(s) 456, 1163
BshTI ACCGGT 1 cut(s) 1226
BsiSI CCGG 3 cut(s) 941, 984, 1227
BslFI GGGAC 2 cut(s) 834, 1112
BslI CCNNNNNNNGG 3 cut(s) 275, 679, 818
BsmAI GTCTC 3 cut(s) 541, 576, 1026
BsmBI CGTCTC 1 cut(s) 541
BsmFI GGGAC 2 cut(s) 834, 1112
BsmI GAATGC 3 cut(s) 13, 472, 661
BsnI GGCC 2 cut(s) 456, 1163
Bsp143I GATC 3 cut(s) 392, 580, 1055
Bsp1720I GCTNAGC 1 cut(s) 483
Bsp19I CCATGG 1 cut(s) 1174
BspACI CCGC 3 cut(s) 327, 422, 1124
BspANI GGCC 2 cut(s) 456, 1163
BspCNI CTCAG 2 cut(s) 475, 975
BspFNI CGCG 1 cut(s) 422
BspHI TCATGA 1 cut(s) 891
BspLI GGNNCC 3 cut(s) 684, 931, 1128
BspPI GGATC 1 cut(s) 400
BspTI CTTAAG 1 cut(s) 997
BsrDI GCAATG 1 cut(s) 1280
BsrFI RCCGGY 1 cut(s) 1226
BsrI ACTGG 2 cut(s) 362, 752
BssAI RCCGGY 1 cut(s) 1226
BssECI CCNNGG 2 cut(s) 939, 1174
BssMI GATC 3 cut(s) 392, 580, 1055
BssT1I CCWWGG 1 cut(s) 1174
Bst2UI CCWGG 1 cut(s) 1205
Bst4CI ACNGT 2 cut(s) 370, 463
BstAFI CTTAAG 1 cut(s) 997
BstAPI GCANNNNNTGC 1 cut(s) 632
BstC8I GCNNGC 4 cut(s) 13, 97, 232, 1165
BstDEI CTNAG 3 cut(s) 483, 945, 962
BstDSI CCRYGG 1 cut(s) 1174
BstF5I GGATG 4 cut(s) 669, 707, 1008, 1119
BstFNI CGCG 1 cut(s) 422
BstHHI GCGC 2 cut(s) 707, 1081
BstKTI GATC 3 cut(s) 395, 583, 1058
BstMAI GTCTC 3 cut(s) 541, 576, 1026
BstMBI GATC 3 cut(s) 392, 580, 1055
BstMWI GCNNNNNNNGC 4 cut(s) 419, 632, 866, 875
BstNI CCWGG 1 cut(s) 1205
BstNSI RCATGY 1 cut(s) 275
BstPAI GACNNNNGTC 1 cut(s) 1018
BstSCI CCNGG 3 cut(s) 939, 982, 1203
BstUI CGCG 1 cut(s) 422
BstV1I GCAGC 6 cut(s) 15, 18, 1063, 1068, 1265, 1285
BstV2I GAAGAC 2 cut(s) 789, 1147
BstX2I RGATCY 1 cut(s) 1055
BstXI CCANNNNNNTGG 1 cut(s) 137
BstYI RGATCY 1 cut(s) 1055
BsuI GTATCC 1 cut(s) 327
BsuRI GGCC 2 cut(s) 456, 1163
BtgI CCRYGG 1 cut(s) 1174
BtgZI GCGATG 1 cut(s) 213
BtsCI GGATG 4 cut(s) 669, 707, 1008, 1119
BtsI GCAGTG 1 cut(s) 1263
BtsIMutI CAGTG 2 cut(s) 1197, 1263
Cac8I GCNNGC 4 cut(s) 13, 97, 232, 1165
CciI TCATGA 1 cut(s) 891
CfoI GCGC 2 cut(s) 707, 1081
Cfr10I RCCGGY 1 cut(s) 1226
Cfr13I GGNCC 3 cut(s) 438, 1126, 1288
CseI GACGC 1 cut(s) 523
Csp6I GTAC 2 cut(s) 799, 949
CspAI ACCGGT 1 cut(s) 1226
CviAII CATG 7 cut(s) 272, 404, 442, 892, 1139, 1175, 1302
CviQI GTAC 2 cut(s) 799, 949
DdeI CTNAG 3 cut(s) 483, 945, 962
DpnI GATC 3 cut(s) 394, 582, 1057
DpnII GATC 3 cut(s) 392, 580, 1055
Eco130I CCWWGG 1 cut(s) 1174
Eco147I AGGCCT 1 cut(s) 1163
Eco47I GGWCC 3 cut(s) 438, 1126, 1288
EcoRII CCWGG 1 cut(s) 1203
EcoT14I CCWWGG 1 cut(s) 1174
EcoT22I ATGCAT 1 cut(s) 472
ErhI CCWWGG 1 cut(s) 1174
Esp3I CGTCTC 1 cut(s) 541
FaeI CATG 7 cut(s) 275, 407, 445, 895, 1142, 1178, 1305
FaqI GGGAC 2 cut(s) 834, 1112
FatI CATG 7 cut(s) 271, 403, 441, 891, 1138, 1174, 1301
FbaI TGATCA 1 cut(s) 580
FblI GTMKAC 1 cut(s) 188
Fnu4HI GCNGC 6 cut(s) 29, 32, 1077, 1082, 1254, 1299
FokI GGATG 4 cut(s) 656, 714, 1015, 1126
Fsp4HI GCNGC 6 cut(s) 29, 32, 1077, 1082, 1254, 1299
GlaI GCGC 2 cut(s) 706, 1080
GluI GCNGC 6 cut(s) 29, 32, 1077, 1082, 1254, 1299
GsaI CCCAGC 1 cut(s) 482
HaeIII GGCC 2 cut(s) 456, 1163
HapII CCGG 3 cut(s) 941, 984, 1227
HgaI GACGC 1 cut(s) 523
HhaI GCGC 2 cut(s) 707, 1081
Hin1II CATG 7 cut(s) 275, 407, 445, 895, 1142, 1178, 1305
Hin6I GCGC 2 cut(s) 705, 1079
HinP1I GCGC 2 cut(s) 705, 1079
HindIII AAGCTT 1 cut(s) 311
HinfI GANTC 9 cut(s) 144, 221, 425, 618, 690, 768, 1019, 1033, 1208
HpaII CCGG 3 cut(s) 941, 984, 1227
HphI GGTGA 2 cut(s) 718, 832
Hpy166II GTNNAC 3 cut(s) 154, 189, 753
Hpy188I TCNGA 2 cut(s) 701, 965
Hpy188III TCNNGA 5 cut(s) 379, 613, 892, 1030, 1098
Hpy8I GTNNAC 3 cut(s) 154, 189, 753
HpyAV CCTTC 3 cut(s) 822, 962, 1178
HpyCH4III ACNGT 2 cut(s) 370, 463
HpyF10VI GCNNNNNNNGC 4 cut(s) 419, 632, 866, 875
HpyF3I CTNAG 3 cut(s) 483, 945, 962
Hsp92II CATG 7 cut(s) 275, 407, 445, 895, 1142, 1178, 1305
HspAI GCGC 2 cut(s) 705, 1079
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 3 cut(s) 392, 580, 1055
LmnI GCTCC 3 cut(s) 688, 875, 929
Lsp1109I GCAGC 6 cut(s) 15, 18, 1063, 1068, 1265, 1285
LweI GCATC 5 cut(s) 190, 479, 618, 622, 692
MaeIII GTNAC 3 cut(s) 364, 769, 986
MalI GATC 3 cut(s) 394, 582, 1057
MboI GATC 3 cut(s) 392, 580, 1055
MboII GAAGA 4 cut(s) 607, 794, 1050, 1147
MfeI CAATTG 1 cut(s) 853
MflI RGATCY 1 cut(s) 1055
MluCI AATT 5 cut(s) 69, 239, 430, 853, 1108
MlyI GAGTC 3 cut(s) 777, 1027, 1028
MmeI TCCRAC 2 cut(s) 235, 697
Mph1103I ATGCAT 1 cut(s) 472
MseI TTAA 1 cut(s) 998
MslI CAYNNNNRTG 1 cut(s) 408
MspA1I CMGCKG 1 cut(s) 482
MspCI CTTAAG 1 cut(s) 997
MspI CCGG 3 cut(s) 941, 984, 1227
MspR9I CCNGG 3 cut(s) 941, 984, 1205
MunI CAATTG 1 cut(s) 853
Mva1269I GAATGC 3 cut(s) 13, 472, 661
MvaI CCWGG 1 cut(s) 1205
MvnI CGCG 1 cut(s) 422
MwoI GCNNNNNNNGC 4 cut(s) 419, 632, 866, 875
NciI CCSGG 2 cut(s) 941, 984
NcoI CCATGG 1 cut(s) 1174
NdeII GATC 3 cut(s) 392, 580, 1055
NlaIII CATG 7 cut(s) 275, 407, 445, 895, 1142, 1178, 1305
NlaIV GGNNCC 3 cut(s) 684, 931, 1128
NmuCI GTSAC 1 cut(s) 769
NsiI ATGCAT 1 cut(s) 472
NspI RCATGY 1 cut(s) 275
PagI TCATGA 1 cut(s) 891
PceI AGGCCT 1 cut(s) 1163
PctI GAATGC 3 cut(s) 13, 472, 661
PfeI GAWTC 6 cut(s) 144, 221, 425, 618, 690, 1208
PflMI CCANNNNNTGG 1 cut(s) 275
PinAI ACCGGT 1 cut(s) 1226
PkrI GCNGC 6 cut(s) 30, 33, 1078, 1083, 1255, 1300
PleI GAGTC 3 cut(s) 776, 1027, 1027
PpsI GAGTC 3 cut(s) 776, 1027, 1027
PshAI GACNNNNGTC 1 cut(s) 1018
Psp6I CCWGG 1 cut(s) 1203
PspFI CCCAGC 1 cut(s) 478
PspGI CCWGG 1 cut(s) 1203
PspN4I GGNNCC 3 cut(s) 684, 931, 1128
PspPI GGNCC 3 cut(s) 438, 1126, 1288
PsuI RGATCY 1 cut(s) 1055
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 2 cut(s) 800, 950
RsaNI GTAC 2 cut(s) 799, 949
RseI CAYNNNNRTG 1 cut(s) 408
SaqAI TTAA 1 cut(s) 998
SatI GCNGC 6 cut(s) 29, 32, 1077, 1082, 1254, 1299
Sau3AI GATC 3 cut(s) 392, 580, 1055
Sau96I GGNCC 3 cut(s) 438, 1126, 1288
SchI GAGTC 3 cut(s) 777, 1027, 1028
ScrFI CCNGG 3 cut(s) 941, 984, 1205
SfaNI GCATC 5 cut(s) 190, 479, 618, 622, 692
SinI GGWCC 3 cut(s) 438, 1126, 1288
SmiMI CAYNNNNRTG 1 cut(s) 408
SmlI CTYRAG 4 cut(s) 175, 446, 997, 1016
SmoI CTYRAG 4 cut(s) 175, 446, 997, 1016
Sse9I AATT 5 cut(s) 69, 239, 430, 853, 1108
SseBI AGGCCT 1 cut(s) 1163
SsiI CCGC 3 cut(s) 327, 422, 1124
StuI AGGCCT 1 cut(s) 1163
StyD4I CCNGG 3 cut(s) 939, 982, 1203
StyI CCWWGG 1 cut(s) 1174
TaaI ACNGT 2 cut(s) 370, 463
TaqI TCGA 2 cut(s) 758, 1158
TasI AATT 5 cut(s) 69, 239, 430, 853, 1108
TfiI GAWTC 6 cut(s) 144, 221, 425, 618, 690, 1208
Tru1I TTAA 1 cut(s) 998
Tru9I TTAA 1 cut(s) 998
TscAI CASTG 2 cut(s) 1204, 1270
TseFI GTSAC 1 cut(s) 769
TseI GCWGC 6 cut(s) 28, 31, 1076, 1081, 1253, 1298
Tsp45I GTSAC 1 cut(s) 769
TspDTI ATGAA 6 cut(s) 388, 392, 725, 806, 880, 1200
TspGWI ACGGA 1 cut(s) 667
TspRI CASTG 2 cut(s) 1204, 1270
Van91I CCANNNNNTGG 1 cut(s) 275
Vha464I CTTAAG 1 cut(s) 997
VpaK11BI GGWCC 3 cut(s) 438, 1126, 1288
XapI RAATTY 1 cut(s) 239
XceI RCATGY 1 cut(s) 275
XcmI CCANNNNNNNNNTGG 1 cut(s) 272
XmiI GTMKAC 1 cut(s) 188
Zsp2I ATGCAT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.