MD10G1206400.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013464
Physical Location & Seq
Reverse (-)
30537161 .. 30540331
3171 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1206400.v1.1.491

Sequence Viewer

Length: 3171 bp
ATGCTCAGGGGAAGGAGAACTGAAGAAGCAAAAGAAGCAGAGCTGGCTATTTGCTGCAGAAAACTCTCAGCTCTGAAACTTGAGCTTCTTCCACTTCTACCCACCTATTCCGCCATTGTTACACGCAGAAGCTATCTAAACCCAAAACCCATCTCTCTCCCACCATGCCCAGAAGAACCCACAAGCTATGGCGGCATTCAGTATCCCCACCTTTCGAACCACCCCAACCCCGAAATCTCAAGCTTTTCTCAAAAGGGTTTCTCTGAGATCACCAGAGACATCAATGGAAGAGCGCTACATGGATTTTGCGTGAAGGGTTTTGTGCCTATTGGTGTGTTCAGTTTGAATACTTTGGTGAATATGTACTCCAAGTTTGGTCGTATTGGTTATGCAAGGTATGTGTTTGATAATATTCCTGAAAGAAATGAAGCTTCTTGGAACACGATGATATCGGGTTATGTACGAGTGGGGTTCTATTCCGAATCGATTGGATTCTTCTGTGAAATGCTTGGCTGCGGTGTTAAGCCAAGTGGGTTTGTCATTGCTAGTTTGATAACTGCGTGTGATAAGTCTGCTTGTAGGTTTAGTGAAGGGTTGCAGGTCCATGGCTTTGTGGTTAAAGTTGGCTTGTTTTGTGATGTCTTTGTTGGGACTTCGCTTTTGCACTTCTATGGCACCTATGGTTTTGTGTCTTCTGCTCAAAAGATTTTTGAGAAGATGCCGGAGAAGAATGTGGTTACTTGGACTTCTTTGATTGTTGGGCATTCAAATAATGGAGAATCAGAAGAAGTTATAAACATATATAAGCGTATGATATATGAAGGAGTGTGCTGCAATGATAACACATTTGCTATAGTTATTAGTACTTGTGGGATGCTGGGGAATGAACTAATAGGTCATCAAGTTCTTGGACATGTCATGAAACTCGGACTAGAGACTAGTGTTTCTGTAGCAAACTCTCTCATATCCATGTATGGTAGCTGTGGTAATGTAGACGAGGCCTGCTATGTCTTTGATCAGATGGATGAGCGTGATATAATCTCATGGAATTCGATTATTTCTGCAAATGCACAAAATGGGCTTTGCGAAGAATCATTGAGGCACTTTCAGTGTATGCGGAGTGTTAATAAAGAGGTGAATTCCACTACACTTTCGACATTGTTGACAGTCTGTGGTTCCTCGGAGAAACTGAAGTGGGGCAGTGGAATCCATGGCTTAGTTGTAAAACTTGGGTTGGAGTCAAATGTCTGTGTTGGCAATACTCTTTTAAGTATGTATTCGGAGGCTGGAAGGTCCAAAGATGCTGAGTTAGTTTTCCAAAGAATGATAGAGAAGGATATAATCTCATGGAACTCGATGTTAGCATGCTATGTTCAGAATGATGAGTATCAAAATGCCTTGGAAATTTTCTCTGAAATGCTTCGGATGAGAAAACCAGTAAATTATGTGACTCTAACAAGTGCACTATCTGCTTGTCCGAACCCTGAATTTCTCATTGAAGGCAAGATTCTTCATGCTCTTGCAGTTTTTGCAGGCCTACAAGACAATCTGATAATTGGCAATGCACTGGTTACAATGTACGGCAAGTTTGGTATGATGGTTGAAGCAGAAAAGGTGCTGCAAATAATGCCCAACCGAGATCAAGTTACTTGGAACGCCCTAATCGGTGGTTACGCTGAGAGCAAAGAACCAAATGAGGCAATAAAAGCTTTCAAGTTCATGAGAGAAGAAGGAACTCCAGCAAACTACATTACAATAATCAATGTCCTTGGTGCTTTTCTGACTCCTAGCAATCTTCTCAAACATGGAATGCCCATCCATGCGCATATAGTTCTAACAGGATTTGATTCGGACAAGCATGTGCAGAGTACCCTCATAACAATGTATGCAAAGTGTGGTGATTTAAATTCAAGTAATTCGATATTCAATGGATTAGCTTTCAAGAACTCTATCTCATGGAATGCACTCATTTCTGCAAATGCTAATCATGGCTTAGAGGAAGCTTTGAAACTTGTAGTGATGATGAGAAAAGTTGGAGTGGACTTGGATCAGTTCAGCCTTTCTGTGGCACTTTCTGTTAGTGCGGACCTGGCTATGTTGGAGGAAGGTCAACAACTTCATGCCTTGGTGGTTAAGCTTGGTTTTGACTCAGACGATTACATTACAAATGCTGCCATGGATATGTATGGAAAATGCGGGGAGATGGAAGATGTTTTGAAATTACTTCCCTTGCCAACCAGCAGGTCACGATTGTCATGGAACATTTTGATATCGTCTTATGCCAAACACGGGTGTTTCCGTAAGGCTAGGGAAGCTTTTCATGAAATGCTAAATCTTGGCATGAAACCCGATCATGTTACCTTTGTTTCACTTCTCTCTGCTTGCAGTCATGGGGGTCTGGTGGATGATGGTCTTGCATACTACAATGCAATGACTACAGAGTTTGGTGTCCCACCAGGAATAGAGCATTGTGTGTGCATAATTGATCTTCTTGGACGATCGGGAAGGCTTGCTGAAGCTGAAAATTTCATTAAAGGAATGGTTGTCCCGCCAAATGACCTTGTGTGGCGGAGCTTGCTGGCTGCATGTAAAATCCATCGTAATGTGGAGTTGGCGAGGAAAGCTGCAGAACATCTCCTAGAGCTAGATCCATCAGACGATTCAGCTTATGTTCTTTTCTCAAATGTATGTGCAACTACTGGTAGATGGGAGGAAGTAGAGAACGTAAGGAGGCAGATGGGATCGAGAAACATAACGAAGAAGCCTGCGTGCAGTTGGGTCAAGTTGAAAGCCGAGGTGAGTAAATTTGGGATGGGAGAGCAGTCCCATCCACAGACAGAGCAAATTTATGCCAAGTTGAGAGAGCTCATGAAGATGATTAGAGAAGCAGGTTATGTCCCGGATACCAGCTACGCACTGCAGGATACAGATGAAGAACAGAAGGAACAAAATCTTTGGAACCACAGTGAGAGGATCGCCCTTGCTTTCGCATTGATCAATACTCCAGAAGGTTCACCAGTTAAGGTTTTCAAGAACCTTCGTGTCTGTGGCGATTGCCATTCTGTCTACAAGCATGTTAGTGCAGTAGTCGGGCAGAAAATCATGTTGAGAGATCCTTACCGGTTTCACCATTTCAGCGACGGCAGGTGTTCTTGTTCTGACTATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

1057

Amino Acids

117.25

Weight (kDa)

6.18

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 142 - 188 8.7e-06 PPR repeat family
PPR PF01535 145 - 174 5.2e-07 PPR repeat
PPR_2 PF13041 243 - 290 3.5e-08 PPR repeat family
PPR_2 PF13041 445 - 492 4e-07 PPR repeat family
PPR PF01535 448 - 476 7.5e-06 PPR repeat
PPR_2 PF13041 547 - 586 3.4e-07 PPR repeat family
PPR PF01535 549 - 578 6.6e-06 PPR repeat
PPR PF01535 752 - 780 2.5e-07 PPR repeat
PPR_2 PF13041 752 - 796 4.9e-11 PPR repeat family
E_motif PF20431 866 - 927 1.5e-22 E motif
DYW_deaminase PF14432 964 - 1056 8e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013464)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16480 AT1G16480
fragaria_vesca FvH4_3g15200
malus_domestica MD10G1206400.v1.1
prunus_persica Prupe.4G135900_v2.0.a1
pyrus_communis pycom10g17620
rosa_chinensis RchiOBHm_Chr5g0025641
rosa_laevigata RLG00000032854
rosa_multiflora Rmu_sc0002137.1_g000002 Rmu_sc0014484.1_g000002
rosa_roxburghii Rroxscaffold_1G00053530 Rroxscaffold_1G00054190
rosa_rugosa Rorug05G0087400
rosa_samantha Rh5AG179200 Rh5BG178300 Rh5CG195900 Rh5DG177500
rosa_wichuraiana Rw5G016270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 794
AarI CACCTGC 1 cut(s) 3135
Acc16I TGCGCA 1 cut(s) 1824
Acc36I ACCTGC 4 cut(s) 589, 2232, 2879, 3135
AccB1I GGYRCC 1 cut(s) 674
AccI GTMKAC 2 cut(s) 993, 3066
AciI CCGC 8 cut(s) 111, 192, 516, 1117, 2084, 2196, 2549, 2569
AclWI GGATC 5 cut(s) 2055, 2642, 2749, 2981, 3107
AcsI RAATTY 8 cut(s) 1048, 1138, 1404, 1487, 1906, 2524, 2804, 2844
AcuI CTGAAG 3 cut(s) 42, 1211, 2535
AfaI GTAC 5 cut(s) 365, 462, 865, 1580, 1870
AfeI AGCGCT 1 cut(s) 294
AfiI CCNNNNNNNGG 2 cut(s) 2065, 2289
AflIII ACRYGT 1 cut(s) 913
AgeI ACCGGT 1 cut(s) 3120
AhlI ACTAGT 1 cut(s) 938
AjnI CCWGG 2 cut(s) 2088, 2455
Alw21I GWGCWC 2 cut(s) 1465, 2868
Alw26I GTCTC 2 cut(s) 270, 929
Alw44I GTGCAC 1 cut(s) 1461
AlwI GGATC 5 cut(s) 2055, 2642, 2749, 2981, 3107
Aor51HI AGCGCT 1 cut(s) 294
AoxI GGCC 2 cut(s) 999, 1534
ApaLI GTGCAC 1 cut(s) 1461
ApeKI GCWGC 7 cut(s) 54, 513, 831, 1618, 2171, 2582, 2624
ApoI RAATTY 8 cut(s) 1048, 1138, 1404, 1487, 1906, 2524, 2804, 2844
ArsI GACNNNNNNTTYG 2 cut(s) 643, 675
AsiGI ACCGGT 1 cut(s) 3120
Asp700I GAANNNNTTC 2 cut(s) 1419, 2316
AspLEI GCGC 2 cut(s) 295, 1825
AspS9I GGNCC 3 cut(s) 601, 1293, 2086
AsuC2I CCSGG 1 cut(s) 2900
AsuHPI GGTGA 7 cut(s) 262, 367, 1147, 1910, 2809, 3006, 3119
AsuII TTCGAA 1 cut(s) 215
AvaII GGWCC 3 cut(s) 601, 1293, 2086
BaeGI GKGCMC 1 cut(s) 1465
BanI GGYRCC 1 cut(s) 674
BanII GRGCYC 1 cut(s) 2868
BbsI GAAGAC 1 cut(s) 684
Bbv12I GWGCWC 2 cut(s) 1465, 2868
BbvI GCAGC 7 cut(s) 41, 500, 818, 1605, 2158, 2569, 2611
BceAI ACGGC 2 cut(s) 1597, 3157
BciT130I CCWGG 2 cut(s) 2090, 2457
BciVI GTATCC 3 cut(s) 213, 2896, 2917
BclI TGATCA 2 cut(s) 1015, 2994
BcnI CCSGG 1 cut(s) 2900
BcoDI GTCTC 2 cut(s) 270, 929
BcuI ACTAGT 1 cut(s) 938
BfaI CTAG 7 cut(s) 546, 932, 939, 1788, 2307, 2639, 2645
BfmI CTRYAG 6 cut(s) 55, 852, 948, 2436, 2625, 2918
BfoI RGCGCY 1 cut(s) 296
BfuAI ACCTGC 4 cut(s) 589, 2232, 2879, 3135
BfuI GTATCC 3 cut(s) 213, 2896, 2917
BisI GCNGC 8 cut(s) 55, 193, 514, 832, 1619, 2172, 2583, 2625
BlsI GCNGC 8 cut(s) 56, 194, 515, 833, 1620, 2173, 2584, 2626
BmcAI AGTACT 1 cut(s) 865
Bme1390I CCNGG 3 cut(s) 2090, 2457, 2900
Bme18I GGWCC 3 cut(s) 601, 1293, 2086
BmgT120I GGNCC 3 cut(s) 601, 1293, 2086
BmiI GGNNCC 3 cut(s) 676, 1177, 2960
BmrFI CCNGG 3 cut(s) 2090, 2457, 2900
BmsI GCATC 3 cut(s) 708, 864, 1291
BpiI GAAGAC 1 cut(s) 684
BpmI CTGGAG 2 cut(s) 1722, 2988
Bpu10I CCTNAGC 1 cut(s) 5
Bpu14I TTCGAA 1 cut(s) 215
BpuEI CTTGAG 2 cut(s) 101, 223
BpuMI CCSGG 1 cut(s) 2900
Bsa29I ATCGAT 1 cut(s) 485
BsaBI GATNNNNATC 1 cut(s) 1386
BsaJI CCNNGG 8 cut(s) 604, 1179, 1210, 1398, 1768, 2124, 2175, 2793
BsaWI WCCGGW 1 cut(s) 3120
Bsc4I CCNNNNNNNGG 2 cut(s) 2065, 2289
Bse118I RCCGGY 1 cut(s) 3120
Bse1I ACTGG 4 cut(s) 1436, 1572, 2704, 3017
Bse3DI GCAATG 4 cut(s) 540, 841, 1567, 2436
Bse8I GATNNNNATC 1 cut(s) 1386
BseBI CCWGG 2 cut(s) 2090, 2457
BseCI ATCGAT 1 cut(s) 485
BseDI CCNNGG 8 cut(s) 604, 1179, 1210, 1398, 1768, 2124, 2175, 2793
BseGI GGATG 7 cut(s) 879, 1030, 1431, 1815, 2410, 2817, 2827
BseJI GATNNNNATC 1 cut(s) 1386
BseLI CCNNNNNNNGG 2 cut(s) 2065, 2289
BseMI GCAATG 4 cut(s) 540, 841, 1567, 2436
BseMII CTCAG 6 cut(s) 19, 81, 255, 1296, 1668, 2163
BseNI ACTGG 4 cut(s) 1436, 1572, 2704, 3017
BseSI GKGCMC 1 cut(s) 1465
BseXI GCAGC 7 cut(s) 41, 500, 818, 1605, 2158, 2569, 2611
BseYI CCCAGC 1 cut(s) 877
BsgI GTGCAG 3 cut(s) 1883, 2791, 3102
Bsh1285I CGRYCG 1 cut(s) 2501
BshFI GGCC 2 cut(s) 1001, 1536
BshNI GGYRCC 1 cut(s) 674
BshTI ACCGGT 1 cut(s) 3120
BshVI ATCGAT 1 cut(s) 485
BsiEI CGRYCG 1 cut(s) 2501
BsiHKAI GWGCWC 2 cut(s) 1465, 2868
BsiSI CCGG 3 cut(s) 722, 2900, 3121
BslFI GGGAC 5 cut(s) 664, 2435, 2531, 2809, 2882
BslI CCNNNNNNNGG 2 cut(s) 2065, 2289
BsmAI GTCTC 2 cut(s) 270, 929
BsmFI GGGAC 5 cut(s) 664, 2435, 2531, 2809, 2882
BsmI GAATGC 4 cut(s) 195, 763, 1815, 1966
BsnI GGCC 2 cut(s) 1001, 1536
Bsp119I TTCGAA 1 cut(s) 215
Bsp1286I GDGCHC 2 cut(s) 1465, 2868
Bsp19I CCATGG 3 cut(s) 604, 1210, 2175
BspACI CCGC 8 cut(s) 111, 192, 516, 1117, 2084, 2196, 2549, 2569
BspANI GGCC 2 cut(s) 1001, 1536
BspCNI CTCAG 6 cut(s) 18, 80, 256, 1297, 1669, 2162
BspDI ATCGAT 1 cut(s) 485
BspHI TCATGA 4 cut(s) 918, 1719, 2320, 2868
BspLI GGNNCC 3 cut(s) 676, 1177, 2960
BspMAI CTGCAG 3 cut(s) 59, 2629, 2922
BspMI ACCTGC 4 cut(s) 589, 2232, 2879, 3135
BspPI GGATC 5 cut(s) 2055, 2642, 2749, 2981, 3107
BspQI GCTCTTC 1 cut(s) 283
BspT104I TTCGAA 1 cut(s) 215
BspT107I GGYRCC 1 cut(s) 674
BsrDI GCAATG 4 cut(s) 540, 841, 1567, 2436
BsrFI RCCGGY 1 cut(s) 3120
BsrI ACTGG 4 cut(s) 1436, 1572, 2704, 3017
BssAI RCCGGY 1 cut(s) 3120
BssECI CCNNGG 8 cut(s) 604, 1179, 1210, 1398, 1768, 2124, 2175, 2793
BssT1I CCWWGG 6 cut(s) 604, 1210, 1398, 1768, 2124, 2175
Bst2UI CCWGG 2 cut(s) 2090, 2457
Bst4CI ACNGT 2 cut(s) 1168, 2966
Bst6I CTCTTC 1 cut(s) 283
BstAPI GCANNNNNTGC 3 cut(s) 1469, 1529, 1627
BstBI TTCGAA 1 cut(s) 215
BstDEI CTNAG 8 cut(s) 5, 67, 264, 1216, 1305, 1677, 1993, 2149
BstDSI CCRYGG 3 cut(s) 604, 1210, 2175
BstF5I GGATG 7 cut(s) 879, 1030, 1431, 1815, 2410, 2817, 2827
BstH2I RGCGCY 1 cut(s) 296
BstHHI GCGC 2 cut(s) 295, 1825
BstMAI GTCTC 2 cut(s) 270, 929
BstMCI CGRYCG 1 cut(s) 2501
BstNI CCWGG 2 cut(s) 2090, 2457
BstNSI RCATGY 5 cut(s) 917, 1368, 1862, 2589, 3077
BstSCI CCNGG 3 cut(s) 2088, 2455, 2898
BstSFI CTRYAG 6 cut(s) 55, 852, 948, 2436, 2625, 2918
BstSLI GKGCMC 1 cut(s) 1465
BstV1I GCAGC 7 cut(s) 41, 500, 818, 1605, 2158, 2569, 2611
BstV2I GAAGAC 1 cut(s) 684
BstX2I RGATCY 2 cut(s) 2647, 3112
BstYI RGATCY 2 cut(s) 2647, 3112
Bsu15I ATCGAT 1 cut(s) 485
BsuI GTATCC 3 cut(s) 213, 2896, 2917
BsuRI GGCC 2 cut(s) 1001, 1536
BsuTUI ATCGAT 1 cut(s) 485
BtgI CCRYGG 3 cut(s) 604, 1210, 2175
BtsCI GGATG 7 cut(s) 879, 1030, 1431, 1815, 2410, 2817, 2827
BtsI GCAGTG 2 cut(s) 1207, 2915
BtsIMutI CAGTG 5 cut(s) 1115, 1207, 1565, 2915, 2971
BveI ACCTGC 4 cut(s) 589, 2232, 2879, 3135
CciI TCATGA 4 cut(s) 918, 1719, 2320, 2868
CfoI GCGC 2 cut(s) 295, 1825
Cfr10I RCCGGY 1 cut(s) 3120
Cfr13I GGNCC 3 cut(s) 601, 1293, 2086
ClaI ATCGAT 1 cut(s) 485
Csp6I GTAC 5 cut(s) 364, 461, 864, 1579, 1869
CspAI ACCGGT 1 cut(s) 3120
CviQI GTAC 5 cut(s) 364, 461, 864, 1579, 1869
DdeI CTNAG 8 cut(s) 5, 67, 264, 1216, 1305, 1677, 1993, 2149
DraI TTTAAA 1 cut(s) 1905
Eam1104I CTCTTC 1 cut(s) 283
EarI CTCTTC 1 cut(s) 283
EciI GGCGGA 2 cut(s) 100, 2584
Ecl136II GAGCTC 1 cut(s) 2866
Eco130I CCWWGG 6 cut(s) 604, 1210, 1398, 1768, 2124, 2175
Eco147I AGGCCT 2 cut(s) 1001, 1536
Eco24I GRGCYC 1 cut(s) 2868
Eco32I GATATC 2 cut(s) 450, 2271
Eco47I GGWCC 3 cut(s) 601, 1293, 2086
Eco47III AGCGCT 1 cut(s) 294
Eco53kI GAGCTC 1 cut(s) 2866
Eco57I CTGAAG 3 cut(s) 42, 1211, 2535
EcoICRI GAGCTC 1 cut(s) 2866
EcoRI GAATTC 2 cut(s) 1048, 1138
EcoRII CCWGG 2 cut(s) 2088, 2455
EcoRV GATATC 2 cut(s) 450, 2271
EcoT14I CCWWGG 6 cut(s) 604, 1210, 1398, 1768, 2124, 2175
EcoT38I GRGCYC 1 cut(s) 2868
ErhI CCWWGG 6 cut(s) 604, 1210, 1398, 1768, 2124, 2175
FaqI GGGAC 5 cut(s) 664, 2435, 2531, 2809, 2882
FauI CCCGC 2 cut(s) 2189, 2556
FbaI TGATCA 2 cut(s) 1015, 2994
FblI GTMKAC 2 cut(s) 993, 3066
Fnu4HI GCNGC 8 cut(s) 55, 193, 514, 832, 1619, 2172, 2583, 2625
FokI GGATG 7 cut(s) 886, 1037, 1438, 1802, 2417, 2814, 2824
FriOI GRGCYC 1 cut(s) 2868
Fsp4HI GCNGC 8 cut(s) 55, 193, 514, 832, 1619, 2172, 2583, 2625
FspAI RTGCGCAY 1 cut(s) 1824
FspBI CTAG 7 cut(s) 546, 932, 939, 1788, 2307, 2639, 2645
FspI TGCGCA 1 cut(s) 1824
GlaI GCGC 2 cut(s) 294, 1824
GluI GCNGC 8 cut(s) 55, 193, 514, 832, 1619, 2172, 2583, 2625
GsaI CCCAGC 1 cut(s) 881
GsuI CTGGAG 2 cut(s) 1722, 2988
HaeII RGCGCY 1 cut(s) 296
HaeIII GGCC 2 cut(s) 1001, 1536
HapII CCGG 3 cut(s) 722, 2900, 3121
HhaI GCGC 2 cut(s) 295, 1825
Hin6I GCGC 2 cut(s) 293, 1823
HinP1I GCGC 2 cut(s) 293, 1823
HincII GTYRAC 2 cut(s) 1164, 2111
HindII GTYRAC 2 cut(s) 1164, 2111
HindIII AAGCTT 6 cut(s) 241, 429, 1707, 2001, 2135, 2313
HpaII CCGG 3 cut(s) 722, 2900, 3121
HphI GGTGA 7 cut(s) 262, 367, 1147, 1910, 2809, 3006, 3119
Hpy166II GTNNAC 7 cut(s) 994, 1164, 1463, 2041, 2111, 3014, 3067
Hpy8I GTNNAC 7 cut(s) 994, 1164, 1463, 2041, 2111, 3014, 3067
Hpy99I CGWCG 1 cut(s) 3143
HpyCH4III ACNGT 2 cut(s) 1168, 2966
HpyCH4IV ACGT 1 cut(s) 2724
HpyF3I CTNAG 8 cut(s) 5, 67, 264, 1216, 1305, 1677, 1993, 2149
HpySE526I ACGT 1 cut(s) 2724
HspAI GCGC 2 cut(s) 293, 1823
Ksp22I TGATCA 2 cut(s) 1015, 2994
LguI GCTCTTC 1 cut(s) 283
LmnI GCTCC 1 cut(s) 2571
Lsp1109I GCAGC 7 cut(s) 41, 500, 818, 1605, 2158, 2569, 2611
LweI GCATC 3 cut(s) 708, 864, 1291
MaeI CTAG 7 cut(s) 546, 932, 939, 1788, 2307, 2639, 2645
MaeII ACGT 1 cut(s) 2724
MaeIII GTNAC 8 cut(s) 118, 736, 1447, 1570, 1645, 1670, 2244, 2356
MflI RGATCY 2 cut(s) 2647, 3112
MhlI GDGCHC 2 cut(s) 1465, 2868
MlyI GAGTC 4 cut(s) 1247, 1444, 1777, 2141
MmeI TCCRAC 3 cut(s) 1215, 2014, 2079
MroXI GAANNNNTTC 2 cut(s) 1419, 2316
MseI TTAA 8 cut(s) 522, 618, 1125, 1268, 1904, 2133, 2532, 3021
MslI CAYNNNNRTG 7 cut(s) 669, 968, 1880, 2180, 2601, 2873, 3078
MspI CCGG 3 cut(s) 722, 2900, 3121
MspR9I CCNGG 3 cut(s) 2090, 2457, 2900
Mva1269I GAATGC 4 cut(s) 195, 763, 1815, 1966
MvaI CCWGG 2 cut(s) 2090, 2457
NciI CCSGG 1 cut(s) 2900
NcoI CCATGG 3 cut(s) 604, 1210, 2175
NlaIV GGNNCC 3 cut(s) 676, 1177, 2960
NmeAIII GCCGAG 1 cut(s) 2818
NmuCI GTSAC 2 cut(s) 1447, 2244
NsbI TGCGCA 1 cut(s) 1824
NspI RCATGY 5 cut(s) 917, 1368, 1862, 2589, 3077
NspV TTCGAA 1 cut(s) 215
PaeI GCATGC 1 cut(s) 1368
PagI TCATGA 4 cut(s) 918, 1719, 2320, 2868
PaqCI CACCTGC 1 cut(s) 3135
PceI AGGCCT 2 cut(s) 1001, 1536
PciI ACATGT 1 cut(s) 913
PciSI GCTCTTC 1 cut(s) 283
PctI GAATGC 4 cut(s) 195, 763, 1815, 1966
PdmI GAANNNNTTC 2 cut(s) 1419, 2316
PfeI GAWTC 8 cut(s) 482, 492, 779, 1091, 1206, 1507, 1847, 2660
PfoI TCCNGGA 1 cut(s) 2898
PinAI ACCGGT 1 cut(s) 3120
PkrI GCNGC 8 cut(s) 56, 194, 515, 833, 1620, 2173, 2584, 2626
Ple19I CGATCG 1 cut(s) 2501
PleI GAGTC 4 cut(s) 1246, 1444, 1777, 2141
PpsI GAGTC 4 cut(s) 1246, 1444, 1777, 2141
PscI ACATGT 1 cut(s) 913
PsiI TTATAA 1 cut(s) 794
Psp124BI GAGCTC 1 cut(s) 2868
Psp6I CCWGG 2 cut(s) 2088, 2455
PspFI CCCAGC 1 cut(s) 877
PspGI CCWGG 2 cut(s) 2088, 2455
PspN4I GGNNCC 3 cut(s) 676, 1177, 2960
PspPI GGNCC 3 cut(s) 601, 1293, 2086
PstI CTGCAG 3 cut(s) 59, 2629, 2922
PsuI RGATCY 2 cut(s) 2647, 3112
PvuI CGATCG 1 cut(s) 2501
RsaI GTAC 5 cut(s) 365, 462, 865, 1580, 1870
RsaNI GTAC 5 cut(s) 364, 461, 864, 1579, 1869
RseI CAYNNNNRTG 7 cut(s) 669, 968, 1880, 2180, 2601, 2873, 3078
SacI GAGCTC 1 cut(s) 2868
SapI GCTCTTC 1 cut(s) 283
SaqAI TTAA 8 cut(s) 522, 618, 1125, 1268, 1904, 2133, 2532, 3021
SatI GCNGC 8 cut(s) 55, 193, 514, 832, 1619, 2172, 2583, 2625
Sau96I GGNCC 3 cut(s) 601, 1293, 2086
ScaI AGTACT 1 cut(s) 865
SchI GAGTC 4 cut(s) 1247, 1444, 1777, 2141
ScrFI CCNGG 3 cut(s) 2090, 2457, 2900
SduI GDGCHC 2 cut(s) 1465, 2868
SfaNI GCATC 3 cut(s) 708, 864, 1291
SfcI CTRYAG 6 cut(s) 55, 852, 948, 2436, 2625, 2918
SfuI TTCGAA 1 cut(s) 215
SinI GGWCC 3 cut(s) 601, 1293, 2086
SmiI ATTTAAAT 1 cut(s) 1905
SmiMI CAYNNNNRTG 7 cut(s) 669, 968, 1880, 2180, 2601, 2873, 3078
SmlI CTYRAG 2 cut(s) 80, 238
SmoI CTYRAG 2 cut(s) 80, 238
SpeI ACTAGT 1 cut(s) 938
SphI GCATGC 1 cut(s) 1368
SseBI AGGCCT 2 cut(s) 1001, 1536
SsiI CCGC 8 cut(s) 111, 192, 516, 1117, 2084, 2196, 2549, 2569
SspI AATATT 1 cut(s) 412
SspMI CTAG 7 cut(s) 546, 932, 939, 1788, 2307, 2639, 2645
SstI GAGCTC 1 cut(s) 2868
StuI AGGCCT 2 cut(s) 1001, 1536
StyD4I CCNGG 3 cut(s) 2088, 2455, 2898
StyI CCWWGG 6 cut(s) 604, 1210, 1398, 1768, 2124, 2175
SwaI ATTTAAAT 1 cut(s) 1905
TaaI ACNGT 2 cut(s) 1168, 2966
TaiI ACGT 1 cut(s) 2727
TaqI TCGA 7 cut(s) 215, 485, 1052, 1154, 1355, 1919, 2744
TatI WGTACW 2 cut(s) 363, 863
TauI GCSGC 1 cut(s) 195
TfiI GAWTC 8 cut(s) 482, 492, 779, 1091, 1206, 1507, 1847, 2660
Tru1I TTAA 8 cut(s) 522, 618, 1125, 1268, 1904, 2133, 2532, 3021
Tru9I TTAA 8 cut(s) 522, 618, 1125, 1268, 1904, 2133, 2532, 3021
TscAI CASTG 5 cut(s) 1115, 1207, 1572, 2922, 2971
TseFI GTSAC 2 cut(s) 1447, 2244
TseI GCWGC 7 cut(s) 54, 513, 831, 1618, 2171, 2582, 2624
Tsp45I GTSAC 2 cut(s) 1447, 2244
TspGWI ACGGA 1 cut(s) 2288
TspRI CASTG 5 cut(s) 1115, 1207, 1572, 2922, 2971
VneI GTGCAC 1 cut(s) 1461
VpaK11BI GGWCC 3 cut(s) 601, 1293, 2086
XapI RAATTY 8 cut(s) 1048, 1138, 1404, 1487, 1906, 2524, 2804, 2844
XceI RCATGY 5 cut(s) 917, 1368, 1862, 2589, 3077
XmiI GTMKAC 2 cut(s) 993, 3066
XmnI GAANNNNTTC 2 cut(s) 1419, 2316
XspI CTAG 7 cut(s) 546, 932, 939, 1788, 2307, 2639, 2645
ZrmI AGTACT 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.