Rh5CG195900

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0013464
Physical Location & Seq
Forward (+)
19488934 .. 19492507
3574 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG195900.1

Sequence Viewer

Length: 2814 bp
ATGTACTCGAAATTTGGTCGTATCGAATATGCACGTAAGGTGTTTGATGATATTCCTGAAAGAAATGAAGCTTCTTGGAACACGATGATGTCGAGCTATGTGAGAGTGGGGTTGTATTCCGACTCGGTTAGGTTCTTTTGTGAAATGGTTGGCTGTGGTGTTGGGCCTAGTGGGTTTGTCATTGCTAGTTTGGTTACTGCGTGTGATAAGTCTGGTTCTATGTTTTGTGAAGGAATGCAAGTTCATGGCTTTGTGGTGAAACTTGGGGGGTTGTTGTGTGATGTGTTTGTGGGAACTTCTGTTTTGCATTTTTATGGGACCTATGGTGTTGTTTCGAATGCTAGAAAGGTGTTTGAGGAGATGCCGGAGAAGAATGTGGTTACTTGGACTTCTTTGATTGTTGGGTATTCTAATGGTGGAGATTTGGGAGAGGTAATGAGTATATATAAGCGTATGAGGCGTGAAGGAGTGTGTTGCAATGATAACACTTTGGCTATAGTGTTTAGTACTTGTGGGGTGCTGGGGGATGAGTTATTGGGTCAGCAAGTTCTTGGAGATGTTGTGAAACTTGGGTTGCAGACTAGTGTTTCGGTGGCAAACTCCCTCATATCCATGTATGGTAGCTTTGGTAATGTGGCCGAGGCATGCTATGTATTTGATCATATGGATGAAAGGGACACAGTCTCATGGAATTCGATTATTTCTGCTAATGTGCAAAATGGGCTTTGTGAAGAATCATTACGGTATTTTCATTGTATGCGGCATGTTAATAAAGAAGTGAATTCTATAACAGTTTCAACATTGTTGGGAGTCTGCGGTTCCACAGATAAACTGAAGTGGGGCAGGGGAATTCATGGACTAGTGGTGAAACTGGGGATGGAATCAAATGTTTGTGTTGGCAATACTCTTTTAAGTATGTATTCTGAGGCTGGAAGATCTGAAGATGCTGAATTAGTTTTCAAAAGAATGATAGAGAAGGATATAATCTCATGGAACTCCATGTTAGCATGCTATGTTCAGAATGAGGAGTCCCAAAATGCCCTCAAACATTTTGCTGAAATGCTTCGGACAAGAAAACCAATAAATTATGTGACTTTAACAAGTGCATTGTCTGCCTGCTCAAATCCGGAATTTGTAGTTGAAGGAAAGATTCTTCATGCTCTAGCAGTCATTGCAGGCCTACAAGACAATCTGATAGTCGGAAATGCATTAGTTACCATATATGGAAAGCTTAGTAAGACGCTTGAAGCAGCGAAGGTGTTGCAAACAATGCCCAAGCGAGATGAAGTTACTTGGAATTCTCTCATCGGTAGTTATGCTGAAAACAAAGAACCAGATGAGGTAATTACAGCTTTTAAACTCATGAGAAAAGAAGGAACTCGAGCAAACTACATTACAATAATTAATGTTCTCGGTGCTTTCCTGATTCCTAGTGATTTACTAAAACATGGGATGCCCATCCATGCATACATAGTTCTAACAGGATTTGAATCAGAAAAGTATGTGCAGAGTTCCCTTATTACGATGTATGCTAAGTGTGGTGATTTGAACTCAAGTAATTCCATATTTAGCGGACTAACTGTCAAGAACTCCATTGCATGGAATGCAATCATTGCTGCAAATGCTAACCATGGCCTTGAGGAAGCTTTGAAACTTGCTTTGATGATGAGAAGAGCTGGAGTGGACTTTGATCAGTTCAGCTTATCTATCGCACTTTCTGTTAGTGCAGACCTGGCTATGTTGGTAGAGGGTCAGCAGCTTCATGGCCTGGTAGTGAAGCTTGGTTTTGACACAGACCATTATATTACAAATGCTGCGATGGATATGTATGGAAAATGCGGGGAGATGGAAGATGTTCTGAAGATACTTCCCTCACCAACCATTAGGTCACGATTATCATGGAACATCATGATATCATCGTTTGCCAAACATGGGTGTTTCGAGAAGGCTAGGGAAAGCTTTCATGAAATGGTAAGTCTGGGTGTGAGACCTGACCATGTCACCTTTGTCTCACTTCTCTCTGCGTGCAGTCATGGGGGTCTCGTGGATGATGGTCTTGCATACTATAATGCAATGACTACAGAGTTTGGTGTCCCACCAGCAATAGAACATTGTGTGTGCGTAATTGATCTTCTTGGGCGATCAGGAAGGCTTTCTGAGGCTGAAAACTTTATTAAAGAAATGCCAGTTCCCCCAAATGACCTTGTGTGGCGGAGCTTGTTGGCTGCATGTAAAATTCATCGCAATTTGAAATTAGGGAGGAAAGCAGCAAACCATCTTCTTGAGTTAGATCCATCAGACGATTCAGCTTATGTTCTTTTCTCAAATGTTTGTGCAAGTACTGGAAGATGGGAGGAAGTAGAGAATGTAAGGAGGCAAATGGGGTTGAGAAACATAACGAAAAAGCCAGCATGTAGTTGGGTCAAGTTGAAAAGTGAGGTGAGTAAATTCGGGATGGGAGAAAAGTCTCACCCAGAGACACAGCAGATTTATGCCAAGTTGGGAGAGCTTATGAAGATGATTAGAGAAGCAGGCTATGTTCCTGATACAAGCTACTCCCTGCAAGATACCGATGAAGAACAGAAGGAGCAAAATCTTTGGAACCACAGTGAGAGAATTGCCCTTGCTTTCGGGTTGATCAATACTCCAGAAGGTTCACCTGTTAAGGTTTTCAAGAATCTTCGAGTCTGTGGTGATTGCCATTCTGTGTACAAGCATGTTAGTGCAGTAGTTGGGCGGAAAATCATATTGAGGGATCCATACCGGTTTCACCATTTCAGTGATGGCAAGTGTTCTTGTTCTGACTATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

937

Amino Acids

103.76

Weight (kDa)

6.26

Isoelectric Point (pI)

36.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 25 - 54 4.9e-06 PPR repeat
PPR_2 PF13041 124 - 171 7.2e-08 PPR repeat family
PPR PF01535 127 - 157 1e-06 PPR repeat
PPR_2 PF13041 326 - 374 6.8e-06 PPR repeat family
PPR PF01535 430 - 459 1e-05 PPR repeat
PPR PF01535 633 - 662 1.4e-07 PPR repeat
PPR_2 PF13041 633 - 677 6.5e-11 PPR repeat family
E_motif PF20431 747 - 808 1.3e-20 E motif
DYW_deaminase PF14432 845 - 937 1.9e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013464)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16480 AT1G16480
fragaria_vesca FvH4_3g15200
malus_domestica MD10G1206400.v1.1
prunus_persica Prupe.4G135900_v2.0.a1
pyrus_communis pycom10g17620
rosa_chinensis RchiOBHm_Chr5g0025641
rosa_laevigata RLG00000032854
rosa_multiflora Rmu_sc0002137.1_g000002 Rmu_sc0014484.1_g000002
rosa_roxburghii Rroxscaffold_1G00053530 Rroxscaffold_1G00054190
rosa_rugosa Rorug05G0087400
rosa_samantha Rh5AG179200 Rh5BG178300 Rh5CG195900 Rh5DG177500
rosa_wichuraiana Rw5G016270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1599
AccIII TCCGGA 1 cut(s) 1126
AciI CCGC 6 cut(s) 760, 816, 1572, 1839, 2212, 2737
AclWI GGATC 3 cut(s) 2285, 2750, 2763
AcoI YGGCCR 1 cut(s) 636
AcsI RAATTY 8 cut(s) 11, 691, 781, 849, 1130, 1297, 2235, 2447
AcuI CTGAAG 3 cut(s) 854, 960, 1880
AfaI GTAC 4 cut(s) 5, 508, 2341, 2711
AfiI CCNNNNNNNGG 2 cut(s) 1599, 1932
AgeI ACCGGT 1 cut(s) 2763
AhdI GACNNNNNGTC 1 cut(s) 1580
AhlI ACTAGT 2 cut(s) 581, 859
AjnI CCWGG 2 cut(s) 1731, 1767
AloI GAACNNNNNNTCC 2 cut(s) 225, 257
Alw26I GTCTC 6 cut(s) 688, 1981, 2014, 2045, 2471, 2472
AlwI GGATC 3 cut(s) 2285, 2750, 2763
Ama87I CYCGRG 1 cut(s) 1380
Aor13HI TCCGGA 1 cut(s) 1126
AoxI GGCC 5 cut(s) 164, 636, 1177, 1633, 1765
ApeKI GCWGC 6 cut(s) 1250, 1616, 1756, 1814, 2225, 2267
ApoI RAATTY 8 cut(s) 11, 691, 781, 849, 1130, 1297, 2235, 2447
ArsI GACNNNNNNTTYG 2 cut(s) 571, 603
AseI ATTAAT 1 cut(s) 1404
AsiGI ACCGGT 1 cut(s) 2763
Asp700I GAANNNNTTC 4 cut(s) 1062, 1854, 1959, 2152
AspS9I GGNCC 2 cut(s) 164, 318
AsuII TTCGAA 1 cut(s) 335
AvaI CYCGRG 1 cut(s) 1380
AvaII GGWCC 1 cut(s) 318
BamHI GGATCC 1 cut(s) 2755
BarI GAAGNNNNNNTAC 4 cut(s) 723, 755, 2115, 2147
BauI CACGAG 1 cut(s) 2042
BbvI GCAGC 6 cut(s) 1262, 1603, 1768, 1801, 2212, 2279
BcgI CGANNNNNNTGC 2 cut(s) 1243, 1277
BciT130I CCWGG 2 cut(s) 1733, 1769
BclI TGATCA 3 cut(s) 658, 1690, 2637
BcoDI GTCTC 6 cut(s) 688, 1981, 2014, 2045, 2471, 2472
BcuI ACTAGT 2 cut(s) 581, 859
BfaI CTAG 8 cut(s) 168, 186, 342, 582, 860, 1163, 1431, 1950
BfmI CTRYAG 2 cut(s) 495, 2079
BglII AGATCT 1 cut(s) 935
BisI GCNGC 7 cut(s) 761, 1251, 1617, 1757, 1815, 2226, 2268
BlsI GCNGC 7 cut(s) 762, 1252, 1618, 1758, 1816, 2227, 2269
BmcAI AGTACT 2 cut(s) 508, 2341
Bme1390I CCNGG 2 cut(s) 1733, 1769
Bme18I GGWCC 1 cut(s) 318
BmeRI GACNNNNNGTC 1 cut(s) 1580
BmeT110I CYCGRG 1 cut(s) 1380
BmgT120I GGNCC 2 cut(s) 164, 318
BmiI GGNNCC 4 cut(s) 319, 820, 2603, 2757
BmrFI CCNGG 2 cut(s) 1733, 1769
BmrI ACTGGG 1 cut(s) 881
BmsI GCATC 3 cut(s) 351, 934, 1443
BmuI ACTGGG 1 cut(s) 881
BpmI CTGGAG 2 cut(s) 1698, 2631
Bpu14I TTCGAA 1 cut(s) 335
BpuEI CTTGAG 3 cut(s) 1537, 1658, 2303
BsaAI YACGTR 1 cut(s) 35
BsaBI GATNNNNATC 2 cut(s) 1457, 1489
BsaI GGTCTC 2 cut(s) 1981, 2045
BsaJI CCNNGG 2 cut(s) 639, 1630
BsaWI WCCGGW 2 cut(s) 1126, 2763
BsaXI ACNNNNNCTCC 2 cut(s) 546, 576
Bsc4I CCNNNNNNNGG 2 cut(s) 1599, 1932
Bse118I RCCGGY 1 cut(s) 2763
Bse1I ACTGG 3 cut(s) 876, 2186, 2347
Bse3DI GCAATG 6 cut(s) 180, 484, 1170, 1593, 1611, 2079
Bse8I GATNNNNATC 2 cut(s) 1457, 1489
BseAI TCCGGA 1 cut(s) 1126
BseBI CCWGG 2 cut(s) 1733, 1769
BseDI CCNNGG 2 cut(s) 639, 1630
BseGI GGATG 7 cut(s) 532, 673, 882, 1458, 1458, 2053, 2460
BseJI GATNNNNATC 2 cut(s) 1457, 1489
BseLI CCNNNNNNNGG 2 cut(s) 1599, 1932
BseMI GCAATG 6 cut(s) 180, 484, 1170, 1593, 1611, 2079
BseMII CTCAG 2 cut(s) 915, 2148
BseNI ACTGG 3 cut(s) 876, 2186, 2347
BseRI GAGGAG 2 cut(s) 371, 1040
BseXI GCAGC 6 cut(s) 1262, 1603, 1768, 1801, 2212, 2279
BseYI CCCAGC 1 cut(s) 520
BsgI GTGCAG 4 cut(s) 1526, 1746, 2047, 2745
BshFI GGCC 5 cut(s) 166, 638, 1179, 1635, 1767
BshTI ACCGGT 1 cut(s) 2763
BsiHKCI CYCGRG 1 cut(s) 1380
BsiSI CCGG 3 cut(s) 365, 1127, 2764
BslFI GGGAC 4 cut(s) 331, 689, 1015, 2078
BslI CCNNNNNNNGG 2 cut(s) 1599, 1932
BsmAI GTCTC 6 cut(s) 688, 1981, 2014, 2045, 2471, 2472
BsmFI GGGAC 4 cut(s) 331, 689, 1015, 2078
BsmI GAATGC 3 cut(s) 240, 343, 1609
BsnI GGCC 5 cut(s) 166, 638, 1179, 1635, 1767
Bso31I GGTCTC 2 cut(s) 1981, 2045
BsoBI CYCGRG 1 cut(s) 1380
Bsp119I TTCGAA 1 cut(s) 335
Bsp13I TCCGGA 1 cut(s) 1126
Bsp1407I TGTACA 1 cut(s) 2709
Bsp143I GATC 8 cut(s) 658, 935, 1690, 2128, 2141, 2290, 2637, 2755
Bsp19I CCATGG 1 cut(s) 1630
BspACI CCGC 6 cut(s) 760, 816, 1572, 1839, 2212, 2737
BspANI GGCC 5 cut(s) 166, 638, 1179, 1635, 1767
BspCNI CTCAG 2 cut(s) 916, 2149
BspEI TCCGGA 1 cut(s) 1126
BspHI TCATGA 3 cut(s) 1362, 1908, 1963
BspLI GGNNCC 4 cut(s) 319, 820, 2603, 2757
BspPI GGATC 3 cut(s) 2285, 2750, 2763
BspQI GCTCTTC 1 cut(s) 1666
BspT104I TTCGAA 1 cut(s) 335
BspTNI GGTCTC 2 cut(s) 1981, 2045
BsrDI GCAATG 6 cut(s) 180, 484, 1170, 1593, 1611, 2079
BsrFI RCCGGY 1 cut(s) 2763
BsrGI TGTACA 1 cut(s) 2709
BsrI ACTGG 3 cut(s) 876, 2186, 2347
BssAI RCCGGY 1 cut(s) 2763
BssECI CCNNGG 2 cut(s) 639, 1630
BssMI GATC 8 cut(s) 658, 935, 1690, 2128, 2141, 2290, 2637, 2755
BssSI CACGAG 1 cut(s) 2042
BssT1I CCWWGG 1 cut(s) 1630
Bst2BI CACGAG 1 cut(s) 2042
Bst2UI CCWGG 2 cut(s) 1733, 1769
Bst4CI ACNGT 5 cut(s) 682, 744, 793, 1582, 2609
Bst6I CTCTTC 1 cut(s) 1666
BstAPI GCANNNNNTGC 5 cut(s) 1112, 1172, 1270, 1604, 1613
BstAUI TGTACA 1 cut(s) 2709
BstBAI YACGTR 1 cut(s) 35
BstBI TTCGAA 1 cut(s) 335
BstC8I GCNNGC 7 cut(s) 646, 1009, 1117, 1177, 2026, 2409, 2533
BstDEI CTNAG 4 cut(s) 924, 1232, 1533, 2157
BstDSI CCRYGG 1 cut(s) 1630
BstF5I GGATG 7 cut(s) 532, 673, 882, 1458, 1458, 2053, 2460
BstKTI GATC 8 cut(s) 661, 938, 1693, 2131, 2144, 2293, 2640, 2758
BstMAI GTCTC 6 cut(s) 688, 1981, 2014, 2045, 2471, 2472
BstMBI GATC 8 cut(s) 658, 935, 1690, 2128, 2141, 2290, 2637, 2755
BstMWI GCNNNNNNNGC 9 cut(s) 457, 721, 1112, 1172, 1270, 1604, 1613, 1622, 1733
BstNI CCWGG 2 cut(s) 1733, 1769
BstNSI RCATGY 6 cut(s) 648, 767, 1011, 2232, 2415, 2720
BstSCI CCNGG 2 cut(s) 1731, 1767
BstSFI CTRYAG 2 cut(s) 495, 2079
BstV1I GCAGC 6 cut(s) 1262, 1603, 1768, 1801, 2212, 2279
BstX2I RGATCY 3 cut(s) 935, 2290, 2755
BstYI RGATCY 3 cut(s) 935, 2290, 2755
BsuRI GGCC 5 cut(s) 166, 638, 1179, 1635, 1767
BtgI CCRYGG 1 cut(s) 1630
BtgZI GCGATG 2 cut(s) 1832, 2225
BtsCI GGATG 7 cut(s) 532, 673, 882, 1458, 1458, 2053, 2460
BtsIMutI CAGTG 2 cut(s) 2614, 2785
Cac8I GCNNGC 7 cut(s) 646, 1009, 1117, 1177, 2026, 2409, 2533
CciI TCATGA 3 cut(s) 1362, 1908, 1963
Cfr10I RCCGGY 1 cut(s) 2763
Cfr13I GGNCC 2 cut(s) 164, 318
CseI GACGC 1 cut(s) 1249
Csp6I GTAC 4 cut(s) 4, 507, 2340, 2710
CspAI ACCGGT 1 cut(s) 2763
CviQI GTAC 4 cut(s) 4, 507, 2340, 2710
DdeI CTNAG 4 cut(s) 924, 1232, 1533, 2157
DpnI GATC 8 cut(s) 660, 937, 1692, 2130, 2143, 2292, 2639, 2757
DpnII GATC 8 cut(s) 658, 935, 1690, 2128, 2141, 2290, 2637, 2755
DraI TTTAAA 1 cut(s) 1357
DriI GACNNNNNGTC 1 cut(s) 1580
EaeI YGGCCR 1 cut(s) 636
Eam1104I CTCTTC 1 cut(s) 1666
Eam1105I GACNNNNNGTC 1 cut(s) 1580
EarI CTCTTC 1 cut(s) 1666
EciI GGCGGA 2 cut(s) 2227, 2752
Eco130I CCWWGG 1 cut(s) 1630
Eco147I AGGCCT 1 cut(s) 1179
Eco31I GGTCTC 2 cut(s) 1981, 2045
Eco32I GATATC 1 cut(s) 1914
Eco47I GGWCC 1 cut(s) 318
Eco57I CTGAAG 3 cut(s) 854, 960, 1880
Eco88I CYCGRG 1 cut(s) 1380
EcoO109I RGGNCCY 1 cut(s) 318
EcoRI GAATTC 4 cut(s) 691, 781, 849, 1297
EcoRII CCWGG 2 cut(s) 1731, 1767
EcoRV GATATC 1 cut(s) 1914
EcoT14I CCWWGG 1 cut(s) 1630
EcoT22I ATGCAT 2 cut(s) 1210, 1468
ErhI CCWWGG 1 cut(s) 1630
FaqI GGGAC 4 cut(s) 331, 689, 1015, 2078
FauI CCCGC 1 cut(s) 1832
FauNDI CATATG 1 cut(s) 663
FbaI TGATCA 3 cut(s) 658, 1690, 2637
Fnu4HI GCNGC 7 cut(s) 761, 1251, 1617, 1757, 1815, 2226, 2268
FokI GGATG 7 cut(s) 539, 680, 889, 1445, 1465, 2060, 2467
Fsp4HI GCNGC 7 cut(s) 761, 1251, 1617, 1757, 1815, 2226, 2268
FspBI CTAG 8 cut(s) 168, 186, 342, 582, 860, 1163, 1431, 1950
GluI GCNGC 7 cut(s) 761, 1251, 1617, 1757, 1815, 2226, 2268
GsaI CCCAGC 1 cut(s) 524
GsuI CTGGAG 2 cut(s) 1698, 2631
HaeIII GGCC 5 cut(s) 166, 638, 1179, 1635, 1767
HapII CCGG 3 cut(s) 365, 1127, 2764
HgaI GACGC 1 cut(s) 1249
HindIII AAGCTT 5 cut(s) 69, 1229, 1644, 1778, 1957
HpaII CCGG 3 cut(s) 365, 1127, 2764
Hpy166II GTNNAC 3 cut(s) 1684, 2657, 2710
Hpy8I GTNNAC 3 cut(s) 1684, 2657, 2710
HpyCH4III ACNGT 5 cut(s) 682, 744, 793, 1582, 2609
HpyCH4IV ACGT 1 cut(s) 34
HpyF10VI GCNNNNNNNGC 9 cut(s) 457, 721, 1112, 1172, 1270, 1604, 1613, 1622, 1733
HpyF3I CTNAG 4 cut(s) 924, 1232, 1533, 2157
HpySE526I ACGT 1 cut(s) 34
Kpn2I TCCGGA 1 cut(s) 1126
Ksp22I TGATCA 3 cut(s) 658, 1690, 2637
Kzo9I GATC 8 cut(s) 658, 935, 1690, 2128, 2141, 2290, 2637, 2755
LguI GCTCTTC 1 cut(s) 1666
LmnI GCTCC 2 cut(s) 2214, 2587
Lsp1109I GCAGC 6 cut(s) 1262, 1603, 1768, 1801, 2212, 2279
LweI GCATC 3 cut(s) 351, 934, 1443
MaeI CTAG 8 cut(s) 168, 186, 342, 582, 860, 1163, 1431, 1950
MaeII ACGT 1 cut(s) 34
MaeIII GTNAC 7 cut(s) 193, 379, 1090, 1213, 1288, 1887, 1999
MalI GATC 8 cut(s) 660, 937, 1692, 2130, 2143, 2292, 2639, 2757
MboI GATC 8 cut(s) 658, 935, 1690, 2128, 2141, 2290, 2637, 2755
MflI RGATCY 3 cut(s) 935, 2290, 2755
MlyI GAGTC 4 cut(s) 116, 819, 1037, 2694
MmeI TCCRAC 2 cut(s) 144, 1180
Mph1103I ATGCAT 2 cut(s) 1210, 1468
MroI TCCGGA 1 cut(s) 1126
MroXI GAANNNNTTC 4 cut(s) 1062, 1854, 1959, 2152
MseI TTAA 7 cut(s) 768, 911, 1097, 1356, 1404, 2175, 2664
MslI CAYNNNNRTG 6 cut(s) 86, 312, 611, 666, 2721, 2778
MspI CCGG 3 cut(s) 365, 1127, 2764
MspR9I CCNGG 2 cut(s) 1733, 1769
Mva1269I GAATGC 3 cut(s) 240, 343, 1609
MvaI CCWGG 2 cut(s) 1733, 1769
MwoI GCNNNNNNNGC 9 cut(s) 457, 721, 1112, 1172, 1270, 1604, 1613, 1622, 1733
NcoI CCATGG 1 cut(s) 1630
NdeI CATATG 1 cut(s) 663
NdeII GATC 8 cut(s) 658, 935, 1690, 2128, 2141, 2290, 2637, 2755
NlaIV GGNNCC 4 cut(s) 319, 820, 2603, 2757
NmeAIII GCCGAG 1 cut(s) 664
NmuCI GTSAC 3 cut(s) 1090, 1887, 1999
NsiI ATGCAT 2 cut(s) 1210, 1468
NspI RCATGY 6 cut(s) 648, 767, 1011, 2232, 2415, 2720
NspV TTCGAA 1 cut(s) 335
PaeI GCATGC 2 cut(s) 648, 1011
PaeR7I CTCGAG 1 cut(s) 1380
PagI TCATGA 3 cut(s) 1362, 1908, 1963
PceI AGGCCT 1 cut(s) 1179
PciSI GCTCTTC 1 cut(s) 1666
PcsI WCGNNNNNNNCGW 1 cut(s) 89
PctI GAATGC 3 cut(s) 240, 343, 1609
PdmI GAANNNNTTC 4 cut(s) 1062, 1854, 1959, 2152
PfeI GAWTC 7 cut(s) 734, 881, 1150, 1426, 1490, 2303, 2677
PflFI GACNNNGTC 2 cut(s) 680, 1997
PflMI CCANNNNNTGG 1 cut(s) 1599
PinAI ACCGGT 1 cut(s) 2763
PkrI GCNGC 7 cut(s) 762, 1252, 1618, 1758, 1816, 2227, 2269
PleI GAGTC 4 cut(s) 116, 818, 1036, 2693
PpsI GAGTC 4 cut(s) 116, 818, 1036, 2693
Ppu21I YACGTR 1 cut(s) 35
PpuMI RGGWCCY 1 cut(s) 318
PshBI ATTAAT 1 cut(s) 1404
Psp5II RGGWCCY 1 cut(s) 318
Psp6I CCWGG 2 cut(s) 1731, 1767
PspFI CCCAGC 1 cut(s) 520
PspGI CCWGG 2 cut(s) 1731, 1767
PspN4I GGNNCC 4 cut(s) 319, 820, 2603, 2757
PspPI GGNCC 2 cut(s) 164, 318
PspPPI RGGWCCY 1 cut(s) 318
PspXI VCTCGAGB 1 cut(s) 1380
PsuI RGATCY 3 cut(s) 935, 2290, 2755
PsyI GACNNNGTC 2 cut(s) 680, 1997
RsaI GTAC 4 cut(s) 5, 508, 2341, 2711
RsaNI GTAC 4 cut(s) 4, 507, 2340, 2710
RseI CAYNNNNRTG 6 cut(s) 86, 312, 611, 666, 2721, 2778
SapI GCTCTTC 1 cut(s) 1666
SaqAI TTAA 7 cut(s) 768, 911, 1097, 1356, 1404, 2175, 2664
SatI GCNGC 7 cut(s) 761, 1251, 1617, 1757, 1815, 2226, 2268
Sau3AI GATC 8 cut(s) 658, 935, 1690, 2128, 2141, 2290, 2637, 2755
Sau96I GGNCC 2 cut(s) 164, 318
ScaI AGTACT 2 cut(s) 508, 2341
SchI GAGTC 4 cut(s) 116, 819, 1037, 2694
ScrFI CCNGG 2 cut(s) 1733, 1769
SfaNI GCATC 3 cut(s) 351, 934, 1443
SfcI CTRYAG 2 cut(s) 495, 2079
Sfr274I CTCGAG 1 cut(s) 1380
SfuI TTCGAA 1 cut(s) 335
SinI GGWCC 1 cut(s) 318
SlaI CTCGAG 1 cut(s) 1380
SmiMI CAYNNNNRTG 6 cut(s) 86, 312, 611, 666, 2721, 2778
SmlI CTYRAG 4 cut(s) 1380, 1552, 1637, 2282
SmoI CTYRAG 4 cut(s) 1380, 1552, 1637, 2282
SpeI ACTAGT 2 cut(s) 581, 859
SphI GCATGC 2 cut(s) 648, 1011
SseBI AGGCCT 1 cut(s) 1179
SsiI CCGC 6 cut(s) 760, 816, 1572, 1839, 2212, 2737
SspMI CTAG 8 cut(s) 168, 186, 342, 582, 860, 1163, 1431, 1950
StuI AGGCCT 1 cut(s) 1179
StyD4I CCNGG 2 cut(s) 1731, 1767
StyI CCWWGG 1 cut(s) 1630
TaaI ACNGT 5 cut(s) 682, 744, 793, 1582, 2609
TaiI ACGT 1 cut(s) 37
TaqI TCGA 8 cut(s) 8, 24, 92, 335, 695, 1381, 1941, 2683
TatI WGTACW 4 cut(s) 3, 506, 2339, 2709
TauI GCSGC 1 cut(s) 763
TfiI GAWTC 7 cut(s) 734, 881, 1150, 1426, 1490, 2303, 2677
Tru1I TTAA 7 cut(s) 768, 911, 1097, 1356, 1404, 2175, 2664
Tru9I TTAA 7 cut(s) 768, 911, 1097, 1356, 1404, 2175, 2664
TscAI CASTG 2 cut(s) 2614, 2785
TseFI GTSAC 3 cut(s) 1090, 1887, 1999
TseI GCWGC 6 cut(s) 1250, 1616, 1756, 1814, 2225, 2267
Tsp45I GTSAC 3 cut(s) 1090, 1887, 1999
TspRI CASTG 2 cut(s) 2614, 2785
Tth111I GACNNNGTC 2 cut(s) 680, 1997
Van91I CCANNNNNTGG 1 cut(s) 1599
VpaK11BI GGWCC 1 cut(s) 318
VspI ATTAAT 1 cut(s) 1404
XapI RAATTY 8 cut(s) 11, 691, 781, 849, 1130, 1297, 2235, 2447
XceI RCATGY 6 cut(s) 648, 767, 1011, 2232, 2415, 2720
XcmI CCANNNNNNNNNTGG 2 cut(s) 2415, 2780
XhoI CTCGAG 1 cut(s) 1380
XmnI GAANNNNTTC 4 cut(s) 1062, 1854, 1959, 2152
XspI CTAG 8 cut(s) 168, 186, 342, 582, 860, 1163, 1431, 1950
ZrmI AGTACT 2 cut(s) 508, 2341
Zsp2I ATGCAT 2 cut(s) 1210, 1468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.