Rw5G016270

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013464
Physical Location & Seq
Forward (+)
20391156 .. 20395755
4600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G016270.1

Sequence Viewer

Length: 3168 bp
ATGGGGATTGGGTCGACAGCTTTGGCGGTGAGGAAGCAAAACGCAGAGCTGGCTAGCTCCTGCAGAAAATTCTCAGCTTTCAGGCTCCACCTTCCAATCCCAAACCCAACTTCTTACCTCCCCATTTCCACACGCAGAACCTACCTAAACCCAAAACCCATCTCCCTCCAATGCCTAGAACAACCCCAAAACCATGGCACCCATCCCCTTTCAGACCACCCCAACCCCGAAATCTCATGCTTTCCTCAAAAGGGTTTCTCCCAAATCACCCAGGAAACCTCCGGCAGATCCCTACACGCATTGTGCGTAAAGGGCATTGTGCCTCTCAGAGCGTTCTGCTCAAACACTCTGGTCAACATGTACTCGAAATTTGGTCGTATTGAATATGCACGTTTGGTGTTTGATGATATTCCTGAGAGAAATGAAGCTTCTTGGAACACGATGATGTCGAGCTATGTGAGAGTGGGGTTGTATTCCGACTCGGTTAGGTTCTTTTGTGAAATGGTTGGCTGCGGCGTTAGGCCGAGTGGGTTTGTCATTGCTAGTTTGGTTACTGCGTGTGATAAGTCTGGTTCTATGTTTTGTGAAGGAATGCAAGTTCATGGCTTTGTGGTGAAACTTGGGTTGTTGTGTGATGTGTTTGTGGGAACTTCTGTTTTGCATTTTTATGGGACCTATGGTGTTGTTTCGAATGCTAGAAAGGTGTTTGAGGAGATGCCGGAGAAGAATGTGGTTACTTGGACTTCTTTGATTGTTGGGTATTCTAATGGTGGAGATTTGGGAGAGGTAATGAGTATATATAAGCGTATGAGGCGTGAAGGAGTGTGTTGCAATGATAACACTTTGGCTATAGTTTTTAGTACTTGTGGGGCGCTGGGGGATGAGTTATTGGGTCAACAAGTTCTTGGAGATGTTGTGAAACTTGGACTGCAGACTAGTGTTTCTGTGGCAAATTCGCTCATATCCATGTATGGTAGCTTTGGTAATGTGGGCGAGGCGTGCTATGTATTTGATCATATGGATGAAAGGGACACAATCTCATGGAATTCGATTATTTCTGCTAATGTGCAAAATGGGTTTTGTGAAGAATCATTACGGTATTTTCATCGTATGCGGCATGTTAATAAAGAAGTGAATTCTATAACAGTTTCGACGATGTTGGGAGTCTGCGGTTCCACAGATAAACTGAAGTGGGGCAGGGGAATTCATGGACTAGTGGTGAAACTGGGGATGGAATCAAATGTTTGTGTTGGCAATACTCTTTTAAGTATGTATTCTGAGGCTGGAAGATCTGAAGATGTTGAATTAGTTTTCAAAAGAATGATAGAGAAGGATATAATCTCATGGAACTCCATGTTAGCATGCTATGTTCAGAATGAGGAGTCCCAAAATGCCCTCAAACATTTTGCTGAAATGCTTCGGACAAGAAAAGCAATAAATTATGTGACTTTAACAAGTGCATTATCTGCCTGTTCAAATCCAGAATTTGTAGTTGAAGGAAAGATTCTTCATGCTCTAGCAGTCATTGCAGGCCTACAAGACAATCTGATAGTCGGAAATGCATTAGTTACCATGTATGGAAAGCTTAGTAAGACGCTTGAAGCAGCGAAGGTGTTGCAAACAATGCCCAAGCGAGATGAAGTTACTTGGAATTCTCTCATCGGTAGTTATGCTGAAAACAAAGAACCAGATGAGGTAATTACAGCTTTTAAACTCATGAGAAAAGAAGGAACTCCAGCAAACTACATTACAATAATTAATGTTCTCGGTGCTTTCCTGATTCCTAGTGATTTACTAAAACATGGGATGCCCATCCATGCATACATAGTTCTAACAGGATTTGAATCAGAAAAGTATGTGCAGAGTTCCCTTATTACGATGTATGCTAAGTGTGGTGATTTGAACTCAAGTAATTCCATATTTAGCGGACTAACTGTCAAGAACTCCATTGCATGGAATGCAATCATTGCTGCAAATGCTAACCATGGCCTTGAGGAAGCTTTGAAACTTGCTTTGATGATGAGAAGAGCTGGAGTGGACTTTGATCAGTTCAGCTTATCTATCGCACTTTCTGTTAGTGCAGACCTGGCTATGTTGGTAGAAGGTCAGCAGCTTCATGGCCTGGTAGTTAAGCTTGGTTTTGACACAGACCATTATATTACAAATGCTGCGATGGATATGTATGGAAAATGCGGGGAGATGGAAGATGTTCTGAAGATGCTTCCCTCACCAACCATTAGGTCACGATTATCATGGAACATTATGATATCATCGTTTGCCAAACATGGGTGTTTTGAGAAGGCTAGGAAGAGCTTTCATGAAATGGTAAGTCTGGGTGTGAAACCTGACCATGTCACCTTTGTCTCACTTCTCTCTGCGTGCAGTCATGGGGGTCTCGTGGATGATGGTCTTGCATACTATAATGCAATGACTACAGAGTTCGGTGTCCCACCAGCAATAGAACATTGTGTGTGCGTAATTGATCTTCTTGGGCGATCAGGAAGGCTTTCTGAGGCTGAAAAATTTATTAAAGAAATGCCAGTCACCCCAAATGACCTTGTGTGGCGGAGCTTGTTGGCTGCATGTAAAATTCATCGCAATTTGGAATTAGGGAGGAAAGCAGCAGACCATCTTCTTGAGTTAGATCCATCAGACGATTCAGCTTATGTTCTTTTCTCAAATGTTTGTGCAAGTACTGGAAGATGGGAGGAAGTAGAGAATGTAAGGAGGCAAATGGGTTCGAGAAACATAACGAAAAAGCCAGCATGTAGTTGGGTCAAGTTGAAAAGTGAGGTGAGTAAATTCGGGATGGGAGAGAAGTCTCACCCACAGACGCAGCAGATTTATGCCAAGTTGGGAGAGCTTATGAAGATGATTAGAGAAGCAGGCTATGTTCCTGATACAAGCTACTCCCTGCAAGATACCGATGAAGAACAAAAGGAGCAAAATCTTTGGAACCACAGTGAGAGAATTGCCCTTGCTTTTGGGTTGATCAATACTCCAGAAGGTTCACCTGTTAAGGTCTTCAAGAATCTTCGAGTCTGTGGTGATTGCCATTCTGTGTACAAGCATGTTAGTGCAGTAGTTGGGCGGAAAATCATATTGAGGGATCCATACCGGTTTCACCATTTCAGTGATGGCAAGTGTTCTTGTTCTGACTATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

1055

Amino Acids

116.87

Weight (kDa)

7.0

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 144 - 173 5.6e-06 PPR repeat
PPR_2 PF13041 242 - 289 7.3e-08 PPR repeat family
PPR PF01535 245 - 275 1.2e-06 PPR repeat
PPR PF01535 751 - 780 2.1e-07 PPR repeat
PPR_2 PF13041 751 - 795 1.5e-11 PPR repeat family
E_motif PF20431 865 - 926 6.1e-22 E motif
DYW_deaminase PF14432 963 - 1055 2.2e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013464)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16480 AT1G16480
fragaria_vesca FvH4_3g15200
malus_domestica MD10G1206400.v1.1
prunus_persica Prupe.4G135900_v2.0.a1
pyrus_communis pycom10g17620
rosa_chinensis RchiOBHm_Chr5g0025641
rosa_laevigata RLG00000032854
rosa_multiflora Rmu_sc0002137.1_g000002 Rmu_sc0014484.1_g000002
rosa_roxburghii Rroxscaffold_1G00053530 Rroxscaffold_1G00054190
rosa_rugosa Rorug05G0087400
rosa_samantha Rh5AG179200 Rh5BG178300 Rh5CG195900 Rh5DG177500
rosa_wichuraiana Rw5G016270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 197
AccB7I CCANNNNNTGG 1 cut(s) 1953
AccI GTMKAC 1 cut(s) 14
AciI CCGC 8 cut(s) 26, 513, 1114, 1170, 1926, 2193, 2566, 3091
AclWI GGATC 4 cut(s) 282, 2639, 3104, 3117
AcuI CTGAAG 3 cut(s) 1208, 1314, 2234
AfaI GTAC 4 cut(s) 362, 862, 2695, 3065
AfiI CCNNNNNNNGG 3 cut(s) 251, 1953, 2286
AflIII ACRYGT 1 cut(s) 357
AgeI ACCGGT 1 cut(s) 3117
AhdI GACNNNNNGTC 1 cut(s) 1934
AhlI ACTAGT 2 cut(s) 935, 1213
AjnI CCWGG 3 cut(s) 270, 2085, 2121
AjuI GAANNNNNNNTTGG 2 cut(s) 94, 126
AloI GAACNNNNNNTCC 2 cut(s) 582, 614
Alw26I GTCTC 3 cut(s) 2368, 2399, 2826
AlwI GGATC 4 cut(s) 282, 2639, 3104, 3117
AoxI GGCC 4 cut(s) 521, 1531, 1987, 2119
ApeKI GCWGC 8 cut(s) 510, 1604, 1970, 2110, 2168, 2579, 2621, 2836
AseI ATTAAT 1 cut(s) 1758
AsiGI ACCGGT 1 cut(s) 3117
Asp700I GAANNNNTTC 3 cut(s) 1416, 2208, 2506
AspLEI GCGC 1 cut(s) 874
AspS9I GGNCC 1 cut(s) 672
AsuII TTCGAA 1 cut(s) 689
AsuNHI GCTAGC 1 cut(s) 53
AvaII GGWCC 1 cut(s) 672
BamHI GGATCC 1 cut(s) 3109
BanI GGYRCC 1 cut(s) 197
BarI GAAGNNNNNNTAC 4 cut(s) 1077, 1109, 2469, 2501
BauI CACGAG 1 cut(s) 2396
BbsI GAAGAC 1 cut(s) 3016
BbvI GCAGC 8 cut(s) 497, 1616, 1957, 2122, 2155, 2566, 2633, 2848
BcgI CGANNNNNNTGC 2 cut(s) 1597, 1631
BciT130I CCWGG 3 cut(s) 272, 2087, 2123
BclI TGATCA 3 cut(s) 1012, 2044, 2991
BcoDI GTCTC 3 cut(s) 2368, 2399, 2826
BcuI ACTAGT 2 cut(s) 935, 1213
BfaI CTAG 9 cut(s) 54, 176, 543, 696, 936, 1214, 1517, 1785, 2304
BfmI CTRYAG 4 cut(s) 61, 849, 929, 2433
BfoI RGCGCY 1 cut(s) 875
BglII AGATCT 1 cut(s) 1289
BmcAI AGTACT 2 cut(s) 862, 2695
Bme1390I CCNGG 3 cut(s) 272, 2087, 2123
Bme18I GGWCC 1 cut(s) 672
BmeRI GACNNNNNGTC 1 cut(s) 1934
BmgT120I GGNCC 1 cut(s) 672
BmiI GGNNCC 6 cut(s) 86, 199, 673, 1174, 2957, 3111
BmrFI CCNGG 3 cut(s) 272, 2087, 2123
BmrI ACTGGG 1 cut(s) 1235
BmsI GCATC 3 cut(s) 705, 1797, 2208
BmtI GCTAGC 1 cut(s) 57
BmuI ACTGGG 1 cut(s) 1235
BpiI GAAGAC 1 cut(s) 3016
BpmI CTGGAG 3 cut(s) 1719, 2052, 2985
Bpu14I TTCGAA 1 cut(s) 689
BpuEI CTTGAG 3 cut(s) 1891, 2012, 2657
BsaBI GATNNNNATC 2 cut(s) 1811, 1843
BsaI GGTCTC 1 cut(s) 2399
BsaJI CCNNGG 3 cut(s) 193, 270, 1984
BsaWI WCCGGW 1 cut(s) 3117
BsaXI ACNNNNNCTCC 2 cut(s) 900, 930
Bsc4I CCNNNNNNNGG 3 cut(s) 251, 1953, 2286
Bse118I RCCGGY 1 cut(s) 3117
Bse1I ACTGG 3 cut(s) 1230, 2540, 2701
Bse3DI GCAATG 6 cut(s) 537, 838, 1524, 1947, 1965, 2433
Bse8I GATNNNNATC 2 cut(s) 1811, 1843
BseBI CCWGG 3 cut(s) 272, 2087, 2123
BseDI CCNNGG 3 cut(s) 193, 270, 1984
BseGI GGATG 8 cut(s) 202, 886, 1027, 1236, 1812, 1812, 2407, 2814
BseJI GATNNNNATC 2 cut(s) 1811, 1843
BseLI CCNNNNNNNGG 3 cut(s) 251, 1953, 2286
BseMI GCAATG 6 cut(s) 537, 838, 1524, 1947, 1965, 2433
BseMII CTCAG 5 cut(s) 87, 340, 405, 1269, 2502
BseNI ACTGG 3 cut(s) 1230, 2540, 2701
BseRI GAGGAG 2 cut(s) 725, 1394
BseXI GCAGC 8 cut(s) 497, 1616, 1957, 2122, 2155, 2566, 2633, 2848
BseYI CCCAGC 1 cut(s) 874
BsgI GTGCAG 4 cut(s) 1880, 2100, 2401, 3099
BshFI GGCC 4 cut(s) 523, 1533, 1989, 2121
BshNI GGYRCC 1 cut(s) 197
BshTI ACCGGT 1 cut(s) 3117
BsiSI CCGG 3 cut(s) 282, 719, 3118
BslFI GGGAC 4 cut(s) 685, 1043, 1369, 2432
BslI CCNNNNNNNGG 3 cut(s) 251, 1953, 2286
BsmAI GTCTC 3 cut(s) 2368, 2399, 2826
BsmFI GGGAC 4 cut(s) 685, 1043, 1369, 2432
BsmI GAATGC 3 cut(s) 597, 697, 1963
BsnI GGCC 4 cut(s) 523, 1533, 1989, 2121
Bso31I GGTCTC 1 cut(s) 2399
Bsp119I TTCGAA 1 cut(s) 689
Bsp1407I TGTACA 1 cut(s) 3063
Bsp143I GATC 9 cut(s) 287, 1012, 1289, 2044, 2482, 2495, 2644, 2991, 3109
Bsp19I CCATGG 2 cut(s) 193, 1984
BspACI CCGC 8 cut(s) 26, 513, 1114, 1170, 1926, 2193, 2566, 3091
BspANI GGCC 4 cut(s) 523, 1533, 1989, 2121
BspCNI CTCAG 5 cut(s) 86, 339, 406, 1270, 2503
BspHI TCATGA 2 cut(s) 1716, 2317
BspLI GGNNCC 6 cut(s) 86, 199, 673, 1174, 2957, 3111
BspMAI CTGCAG 2 cut(s) 65, 933
BspOI GCTAGC 1 cut(s) 57
BspPI GGATC 4 cut(s) 282, 2639, 3104, 3117
BspQI GCTCTTC 2 cut(s) 2020, 2303
BspT104I TTCGAA 1 cut(s) 689
BspT107I GGYRCC 1 cut(s) 197
BspTNI GGTCTC 1 cut(s) 2399
BsrDI GCAATG 6 cut(s) 537, 838, 1524, 1947, 1965, 2433
BsrFI RCCGGY 1 cut(s) 3117
BsrGI TGTACA 1 cut(s) 3063
BsrI ACTGG 3 cut(s) 1230, 2540, 2701
BssAI RCCGGY 1 cut(s) 3117
BssECI CCNNGG 3 cut(s) 193, 270, 1984
BssMI GATC 9 cut(s) 287, 1012, 1289, 2044, 2482, 2495, 2644, 2991, 3109
BssSI CACGAG 1 cut(s) 2396
BssT1I CCWWGG 2 cut(s) 193, 1984
Bst2BI CACGAG 1 cut(s) 2396
Bst2UI CCWGG 3 cut(s) 272, 2087, 2123
Bst4CI ACNGT 4 cut(s) 1098, 1147, 1936, 2963
Bst6I CTCTTC 2 cut(s) 2020, 2303
BstAPI GCANNNNNTGC 5 cut(s) 1466, 1526, 1624, 1958, 1967
BstAUI TGTACA 1 cut(s) 3063
BstBI TTCGAA 1 cut(s) 689
BstC8I GCNNGC 8 cut(s) 51, 55, 1000, 1363, 1531, 2380, 2763, 2887
BstDEI CTNAG 7 cut(s) 73, 326, 414, 1278, 1586, 1887, 2511
BstDSI CCRYGG 2 cut(s) 193, 1984
BstF5I GGATG 8 cut(s) 202, 886, 1027, 1236, 1812, 1812, 2407, 2814
BstH2I RGCGCY 1 cut(s) 875
BstHHI GCGC 1 cut(s) 874
BstKTI GATC 9 cut(s) 290, 1015, 1292, 2047, 2485, 2498, 2647, 2994, 3112
BstMAI GTCTC 3 cut(s) 2368, 2399, 2826
BstMBI GATC 9 cut(s) 287, 1012, 1289, 2044, 2482, 2495, 2644, 2991, 3109
BstNI CCWGG 3 cut(s) 272, 2087, 2123
BstNSI RCATGY 6 cut(s) 361, 1121, 1365, 2586, 2769, 3074
BstSCI CCNGG 3 cut(s) 270, 2085, 2121
BstSFI CTRYAG 4 cut(s) 61, 849, 929, 2433
BstV1I GCAGC 8 cut(s) 497, 1616, 1957, 2122, 2155, 2566, 2633, 2848
BstV2I GAAGAC 1 cut(s) 3016
BstX2I RGATCY 4 cut(s) 287, 1289, 2644, 3109
BstXI CCANNNNNNTGG 1 cut(s) 194
BstYI RGATCY 4 cut(s) 287, 1289, 2644, 3109
BsuRI GGCC 4 cut(s) 523, 1533, 1989, 2121
BtgI CCRYGG 2 cut(s) 193, 1984
BtgZI GCGATG 2 cut(s) 2186, 2579
BtsCI GGATG 8 cut(s) 202, 886, 1027, 1236, 1812, 1812, 2407, 2814
BtsIMutI CAGTG 2 cut(s) 2968, 3139
Cac8I GCNNGC 8 cut(s) 51, 55, 1000, 1363, 1531, 2380, 2763, 2887
CciI TCATGA 2 cut(s) 1716, 2317
CfoI GCGC 1 cut(s) 874
Cfr10I RCCGGY 1 cut(s) 3117
Cfr13I GGNCC 1 cut(s) 672
CseI GACGC 2 cut(s) 1603, 2842
Csp6I GTAC 4 cut(s) 361, 861, 2694, 3064
CspAI ACCGGT 1 cut(s) 3117
CviQI GTAC 4 cut(s) 361, 861, 2694, 3064
DdeI CTNAG 7 cut(s) 73, 326, 414, 1278, 1586, 1887, 2511
DpnI GATC 9 cut(s) 289, 1014, 1291, 2046, 2484, 2497, 2646, 2993, 3111
DpnII GATC 9 cut(s) 287, 1012, 1289, 2044, 2482, 2495, 2644, 2991, 3109
DraI TTTAAA 1 cut(s) 1711
DriI GACNNNNNGTC 1 cut(s) 1934
Eam1104I CTCTTC 2 cut(s) 2020, 2303
Eam1105I GACNNNNNGTC 1 cut(s) 1934
EarI CTCTTC 2 cut(s) 2020, 2303
EciI GGCGGA 2 cut(s) 2581, 3106
Eco130I CCWWGG 2 cut(s) 193, 1984
Eco147I AGGCCT 1 cut(s) 1533
Eco31I GGTCTC 1 cut(s) 2399
Eco32I GATATC 1 cut(s) 2268
Eco47I GGWCC 1 cut(s) 672
Eco57I CTGAAG 3 cut(s) 1208, 1314, 2234
EcoO109I RGGNCCY 1 cut(s) 672
EcoRI GAATTC 4 cut(s) 1045, 1135, 1203, 1651
EcoRII CCWGG 3 cut(s) 270, 2085, 2121
EcoRV GATATC 1 cut(s) 2268
EcoT14I CCWWGG 2 cut(s) 193, 1984
EcoT22I ATGCAT 2 cut(s) 1564, 1822
ErhI CCWWGG 2 cut(s) 193, 1984
FaqI GGGAC 4 cut(s) 685, 1043, 1369, 2432
FauI CCCGC 1 cut(s) 2186
FauNDI CATATG 1 cut(s) 1017
FbaI TGATCA 3 cut(s) 1012, 2044, 2991
FblI GTMKAC 1 cut(s) 14
FokI GGATG 8 cut(s) 189, 893, 1034, 1243, 1799, 1819, 2414, 2821
FspBI CTAG 9 cut(s) 54, 176, 543, 696, 936, 1214, 1517, 1785, 2304
GlaI GCGC 1 cut(s) 873
GsaI CCCAGC 1 cut(s) 878
GsuI CTGGAG 3 cut(s) 1719, 2052, 2985
HaeII RGCGCY 1 cut(s) 875
HaeIII GGCC 4 cut(s) 523, 1533, 1989, 2121
HapII CCGG 3 cut(s) 282, 719, 3118
HgaI GACGC 2 cut(s) 1603, 2842
HhaI GCGC 1 cut(s) 874
Hin6I GCGC 1 cut(s) 872
HinP1I GCGC 1 cut(s) 872
HincII GTYRAC 3 cut(s) 15, 355, 896
HindII GTYRAC 3 cut(s) 15, 355, 896
HindIII AAGCTT 4 cut(s) 426, 1583, 1998, 2132
HpaII CCGG 3 cut(s) 282, 719, 3118
Hpy166II GTNNAC 6 cut(s) 15, 355, 896, 2038, 3011, 3064
Hpy8I GTNNAC 6 cut(s) 15, 355, 896, 2038, 3011, 3064
Hpy99I CGWCG 1 cut(s) 1156
HpyCH4III ACNGT 4 cut(s) 1098, 1147, 1936, 2963
HpyCH4IV ACGT 1 cut(s) 391
HpyF3I CTNAG 7 cut(s) 73, 326, 414, 1278, 1586, 1887, 2511
HpySE526I ACGT 1 cut(s) 391
HspAI GCGC 1 cut(s) 872
Ksp22I TGATCA 3 cut(s) 1012, 2044, 2991
Kzo9I GATC 9 cut(s) 287, 1012, 1289, 2044, 2482, 2495, 2644, 2991, 3109
LguI GCTCTTC 2 cut(s) 2020, 2303
LmnI GCTCC 4 cut(s) 62, 90, 2568, 2941
Lsp1109I GCAGC 8 cut(s) 497, 1616, 1957, 2122, 2155, 2566, 2633, 2848
LweI GCATC 3 cut(s) 705, 1797, 2208
MaeI CTAG 9 cut(s) 54, 176, 543, 696, 936, 1214, 1517, 1785, 2304
MaeII ACGT 1 cut(s) 391
MaeIII GTNAC 8 cut(s) 550, 733, 1444, 1567, 1642, 2241, 2353, 2542
MalI GATC 9 cut(s) 289, 1014, 1291, 2046, 2484, 2497, 2646, 2993, 3111
MboI GATC 9 cut(s) 287, 1012, 1289, 2044, 2482, 2495, 2644, 2991, 3109
MflI RGATCY 4 cut(s) 287, 1289, 2644, 3109
MlyI GAGTC 4 cut(s) 473, 1173, 1391, 3048
MmeI TCCRAC 2 cut(s) 501, 1534
Mph1103I ATGCAT 2 cut(s) 1564, 1822
MroXI GAANNNNTTC 3 cut(s) 1416, 2208, 2506
MseI TTAA 8 cut(s) 1122, 1265, 1451, 1710, 1758, 2130, 2529, 3018
MslI CAYNNNNRTG 6 cut(s) 443, 666, 965, 1020, 3075, 3132
MspI CCGG 3 cut(s) 282, 719, 3118
MspR9I CCNGG 3 cut(s) 272, 2087, 2123
Mva1269I GAATGC 3 cut(s) 597, 697, 1963
MvaI CCWGG 3 cut(s) 272, 2087, 2123
NcoI CCATGG 2 cut(s) 193, 1984
NdeI CATATG 1 cut(s) 1017
NdeII GATC 9 cut(s) 287, 1012, 1289, 2044, 2482, 2495, 2644, 2991, 3109
NheI GCTAGC 1 cut(s) 53
NlaIV GGNNCC 6 cut(s) 86, 199, 673, 1174, 2957, 3111
NmeAIII GCCGAG 1 cut(s) 549
NmuCI GTSAC 4 cut(s) 1444, 2241, 2353, 2542
NsiI ATGCAT 2 cut(s) 1564, 1822
NspI RCATGY 6 cut(s) 361, 1121, 1365, 2586, 2769, 3074
NspV TTCGAA 1 cut(s) 689
PaeI GCATGC 1 cut(s) 1365
PagI TCATGA 2 cut(s) 1716, 2317
PceI AGGCCT 1 cut(s) 1533
PciI ACATGT 1 cut(s) 357
PciSI GCTCTTC 2 cut(s) 2020, 2303
PcsI WCGNNNNNNNCGW 2 cut(s) 303, 446
PctI GAATGC 3 cut(s) 597, 697, 1963
PdmI GAANNNNTTC 3 cut(s) 1416, 2208, 2506
PfeI GAWTC 7 cut(s) 1088, 1235, 1504, 1780, 1844, 2657, 3031
PflFI GACNNNGTC 1 cut(s) 2351
PflMI CCANNNNNTGG 1 cut(s) 1953
PinAI ACCGGT 1 cut(s) 3117
PleI GAGTC 4 cut(s) 473, 1172, 1390, 3047
PpsI GAGTC 4 cut(s) 473, 1172, 1390, 3047
PpuMI RGGWCCY 1 cut(s) 672
PscI ACATGT 1 cut(s) 357
PshBI ATTAAT 1 cut(s) 1758
Psp5II RGGWCCY 1 cut(s) 672
Psp6I CCWGG 3 cut(s) 270, 2085, 2121
PspFI CCCAGC 1 cut(s) 874
PspGI CCWGG 3 cut(s) 270, 2085, 2121
PspN4I GGNNCC 6 cut(s) 86, 199, 673, 1174, 2957, 3111
PspPI GGNCC 1 cut(s) 672
PspPPI RGGWCCY 1 cut(s) 672
PstI CTGCAG 2 cut(s) 65, 933
PsuI RGATCY 4 cut(s) 287, 1289, 2644, 3109
PsyI GACNNNGTC 1 cut(s) 2351
RsaI GTAC 4 cut(s) 362, 862, 2695, 3065
RsaNI GTAC 4 cut(s) 361, 861, 2694, 3064
RseI CAYNNNNRTG 6 cut(s) 443, 666, 965, 1020, 3075, 3132
SalI GTCGAC 1 cut(s) 13
SapI GCTCTTC 2 cut(s) 2020, 2303
SaqAI TTAA 8 cut(s) 1122, 1265, 1451, 1710, 1758, 2130, 2529, 3018
Sau3AI GATC 9 cut(s) 287, 1012, 1289, 2044, 2482, 2495, 2644, 2991, 3109
Sau96I GGNCC 1 cut(s) 672
ScaI AGTACT 2 cut(s) 862, 2695
SchI GAGTC 4 cut(s) 473, 1173, 1391, 3048
ScrFI CCNGG 3 cut(s) 272, 2087, 2123
SfaNI GCATC 3 cut(s) 705, 1797, 2208
SfcI CTRYAG 4 cut(s) 61, 849, 929, 2433
SfuI TTCGAA 1 cut(s) 689
SinI GGWCC 1 cut(s) 672
SmiMI CAYNNNNRTG 6 cut(s) 443, 666, 965, 1020, 3075, 3132
SmlI CTYRAG 3 cut(s) 1906, 1991, 2636
SmoI CTYRAG 3 cut(s) 1906, 1991, 2636
SpeI ACTAGT 2 cut(s) 935, 1213
SphI GCATGC 1 cut(s) 1365
SseBI AGGCCT 1 cut(s) 1533
SsiI CCGC 8 cut(s) 26, 513, 1114, 1170, 1926, 2193, 2566, 3091
SspMI CTAG 9 cut(s) 54, 176, 543, 696, 936, 1214, 1517, 1785, 2304
StuI AGGCCT 1 cut(s) 1533
StyD4I CCNGG 3 cut(s) 270, 2085, 2121
StyI CCWWGG 2 cut(s) 193, 1984
TaaI ACNGT 4 cut(s) 1098, 1147, 1936, 2963
TaiI ACGT 1 cut(s) 394
TaqI TCGA 8 cut(s) 14, 365, 449, 689, 1049, 1151, 2741, 3037
TatI WGTACW 4 cut(s) 360, 860, 2693, 3063
TauI GCSGC 2 cut(s) 516, 1117
TfiI GAWTC 7 cut(s) 1088, 1235, 1504, 1780, 1844, 2657, 3031
Tru1I TTAA 8 cut(s) 1122, 1265, 1451, 1710, 1758, 2130, 2529, 3018
Tru9I TTAA 8 cut(s) 1122, 1265, 1451, 1710, 1758, 2130, 2529, 3018
TscAI CASTG 2 cut(s) 2968, 3139
TseFI GTSAC 4 cut(s) 1444, 2241, 2353, 2542
TseI GCWGC 8 cut(s) 510, 1604, 1970, 2110, 2168, 2579, 2621, 2836
Tsp45I GTSAC 4 cut(s) 1444, 2241, 2353, 2542
TspRI CASTG 2 cut(s) 2968, 3139
Tth111I GACNNNGTC 1 cut(s) 2351
Van91I CCANNNNNTGG 1 cut(s) 1953
VpaK11BI GGWCC 1 cut(s) 672
VspI ATTAAT 1 cut(s) 1758
XceI RCATGY 6 cut(s) 361, 1121, 1365, 2586, 2769, 3074
XcmI CCANNNNNNNNNTGG 2 cut(s) 2769, 3134
XmiI GTMKAC 1 cut(s) 14
XmnI GAANNNNTTC 3 cut(s) 1416, 2208, 2506
XspI CTAG 9 cut(s) 54, 176, 543, 696, 936, 1214, 1517, 1785, 2304
ZrmI AGTACT 2 cut(s) 862, 2695
Zsp2I ATGCAT 2 cut(s) 1564, 1822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.