RchiOBHm_Chr5g0025641

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013464
Physical Location & Seq
Forward (+)
19551431 .. 19554625
3195 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30527

Sequence Viewer

Length: 2811 bp
ATGTACTCGAAATTTGGACGTATCGAATATGCACGTAAGGTGTTTGATGATATTCCTGAGAGAAATGAAGCTTCTTGGAACACGATGATGTCGAGCTATGTGAGAGTGGGGTTGTATTCCGACTCGGTTAGGTTCTTTTGTAAGATGGTTGGCTGTGGTGTTGGGCCTAGTGGGTTTGTCATTGCTAGTTTGGTTACTGCGTGTGATAAGTCTGGTTCTATGTTTTGTGAAGGAATGCAAGTCCATGGCTTTGTGGTGAAGATTGGGTTGTTGTGTGATGTGTTTGTGGGTACTTCTGTTTTGCATTTTTATGGGACCTATGGTGTTGTTTCGAATGCTAGAAAGGTGTTTGAGGAGATGCCAGATAAGAATGTGGTTACTTGGACTTCTTTGATTGTTGGGTATTCAAATGGTGGAGATTTGGGAGAGGTAATGAGTATATATAAGCGTATGAGGCGTGAAGGAGTGTGTTGCAATGATAACACTTTGGCTATAGTGTTTAGTACTTGTGGGGCGCTGGGGGATGAGTTATTGGGTCAACAAGTTCTTGGAGTTGCTGTGAAACTTGGACTGCAGACTAGTGTTTCTGTGGCAAATTCGCTCATATCCATGTATGGTAGCTTTGGTAATGTGGCCGAGGCATGCTATGTATTTGATCATATGGATGAAAGGGACACAGTCTCATGGAATTCGATTATTTCTGCTAATGTGCAAAATGGGCTTTGTGAAGAATCATTACGGTATTTTCATCGTATGCGGCATGTTAATAAAGAAGTGAATTCTATAACAGTTTCGACGTTGTTGGGAGTCTGCGGTTCCACAGATAAACTGAAGTGGGGCAGGGGAATTCATGGACTAGTGGTGAAACTGGGGATGGAATCAAATGTTTGTGTTGGCAATACTCTTTTAAGTATGTATTCTGAGGCTGGAAGATCTGAAGATGCTGAATTAGTTTTCAAAAGAATGATAGAGAAGGATATAATCTCATGGAACTCCATGTTAGCATGCTATGTTCAGAATGAGGAGTCCCAAAATGGCCTCAAACATTTTGCTGAAATGCTTCGGACAAGAAAACCAATAAATTATGTGACTTTAACAAGTGCATTGTCTGCCTGCTCAAATCCGGAATTTGTAGTTGAAGGAAAGATTCTTCATGCTCTAGCAGTCATTGCAGGCCTACAAGACAATCTGATAGTCGGAAATGCATTAGTTACCATGTATGGAAAGCTTAGTAAGACGCTTGAAGCAGCGAAGGTGTTGCAAACAATGCCCAAGCGAGATGAAGTTACTTGGAATTCTCTCATCGGTAGTTATGCTGAAAACAAAGAACCAGATGAGGTAATTACAGCTTTTAAACTCATGAGAAAAGAAGGAACTCCAGCAAACTACATTACAATAATTAATGTTCTCGGTGCTTTCCTGATTCCTAGTGATTTACTAAAACATGGGATGCCCATCCATGCATACATAGTTCTAACAGGATTTGAATCAGAAAAGTATGTGCAGAGTTCCCTTATTACGATGTATGCTAAGTGTGGTGATTTGAACTCAAGTAATTCCATATTTAGCGGACTAACTGTCAAGAACTCCATTGCATGGAATGCAATCATTGCTGCAAATGCTAACCATGGCCTTGAGGAAGCTTTGAAACTTGCTTTGATGATGAGAAGAGCTGGAGTGGACTTTGATCAGTTCAGCTTATCTATCGCACTTTCTGTTAGTGCAGACCTGGCTATGTTGGTAGAAGGTCAGCAGCTTCATGGCCTGGTAGTTAAGCTTGGTTTTGACACAGACCATTATATTACAAATGCTGCGATGGATATGTATGGAAAATGCGGGGAGATGGAAGATGTTCTGAAGATGCTTCCCTCACCAACCATTAGGTCACGATTATCATGGAACATCATGATATCATCGTTTGCCAAACATGGGTGTTTCGAGAAGGCCAGGGAGAGCTTTCATGAAATGGTAAGTCTGGGTGTGAAACCTGACCATGTCACCTTTGTCTCACTTCTCTCTGCGTGCAGTCATGGGGGTCTCGTGGATGATGGTCTTGCATACTATAATGCAATGACTACAGAGTTCGGTGTCCCACCGGCAATAGAACATTGTGTGTGCGTAATTGATCTTCTTGGGCGATCAGGAAGGCTTTCTGAGGCTGAAAAATTTATTAAAGAAATGCCAGTCACCCCAAATGACCTTGTGTGGCGGAGCTTGTTGGCTGCATGTAAAATTCATCGCAATTTGGAATTAGGGAGGAAAGCAGCAGACCATCTTCTTGAGTTAGATCCATCAGACGATTCAGCTTATGTTCTTTTCTCAAATGTTTGTGCAAGTACTGGAAGATGGGAGGAAGTAGAGAATGTAAGGAGGCAAATGGGTTCGAGAAACATAACGAAAAAGCCAGCATGTAGTTGGGTCAAGTTGAAAAGTGAGGTGAGTAAATTCGGGATGGGAGAGAAGTCTCATCCACAGACACAGCAGATTTATGCCAAGTTGGGAGCGCTTATGAAGATGATTAGAGAAGCAGGCTATGTTCCTGATACAAGCTACTCCCTGCAAGATACCGATGAAGAACAAAAGGAGCAAAATCTTTGGAACCACAGTGAGAGAATTGCCCTTGCTTTTGGGTTGATCAATACTCCAGAAGGTTCACCTGTTAAGGTCTTCAAGAATCTTCGAGTCTGTGGTGATTGCCATTCTGTGTACAAGCATGTTAGTGCAGTAGTTGGGCGGAAAATCATATTGAGGGATCCATACCGGTTTCACCATTTCAGTGATGGCAAGTGTTCTTGTTCTGACTATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

936

Amino Acids

103.54

Weight (kDa)

6.45

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 123 - 170 6.4e-08 PPR repeat family
PPR PF01535 126 - 156 1e-06 PPR repeat
PPR PF01535 429 - 458 1e-05 PPR repeat
PPR PF01535 632 - 661 1.4e-07 PPR repeat
PPR_2 PF13041 632 - 676 2.6e-11 PPR repeat family
E_motif PF20431 746 - 807 5.3e-22 E motif
DYW_deaminase PF14432 844 - 936 1.9e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013464)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16480 AT1G16480
fragaria_vesca FvH4_3g15200
malus_domestica MD10G1206400.v1.1
prunus_persica Prupe.4G135900_v2.0.a1
pyrus_communis pycom10g17620
rosa_chinensis RchiOBHm_Chr5g0025641
rosa_laevigata RLG00000032854
rosa_multiflora Rmu_sc0002137.1_g000002 Rmu_sc0014484.1_g000002
rosa_roxburghii Rroxscaffold_1G00053530 Rroxscaffold_1G00054190
rosa_rugosa Rorug05G0087400
rosa_samantha Rh5AG179200 Rh5BG178300 Rh5CG195900 Rh5DG177500
rosa_wichuraiana Rw5G016270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1596
AccIII TCCGGA 1 cut(s) 1123
AciI CCGC 6 cut(s) 757, 813, 1569, 1836, 2209, 2734
AclWI GGATC 3 cut(s) 2282, 2747, 2760
AcoI YGGCCR 1 cut(s) 633
AcuI CTGAAG 3 cut(s) 851, 957, 1877
AfaI GTAC 5 cut(s) 5, 292, 505, 2338, 2708
AfeI AGCGCT 1 cut(s) 2505
AfiI CCNNNNNNNGG 2 cut(s) 1596, 1929
AgeI ACCGGT 1 cut(s) 2760
AgsI TTSAA 9 cut(s) 408, 958, 1139, 1244, 1487, 1546, 1648, 2428, 2671
AhdI GACNNNNNGTC 1 cut(s) 1577
AhlI ACTAGT 2 cut(s) 578, 856
AjnI CCWGG 3 cut(s) 1728, 1764, 1946
Alw26I GTCTC 4 cut(s) 685, 2011, 2042, 2469
AlwI GGATC 3 cut(s) 2282, 2747, 2760
Aor13HI TCCGGA 1 cut(s) 1123
Aor51HI AGCGCT 1 cut(s) 2505
AoxI GGCC 7 cut(s) 164, 633, 1036, 1174, 1630, 1762, 1944
ApeKI GCWGC 6 cut(s) 1247, 1613, 1753, 1811, 2222, 2264
AseI ATTAAT 1 cut(s) 1401
AsiGI ACCGGT 1 cut(s) 2760
Asp700I GAANNNNTTC 3 cut(s) 1059, 1851, 2149
AspLEI GCGC 2 cut(s) 517, 2506
AspS9I GGNCC 2 cut(s) 164, 315
AsuII TTCGAA 1 cut(s) 332
AvaII GGWCC 1 cut(s) 315
BaeI ACNNNNGTAYC 2 cut(s) 282, 315
BamHI GGATCC 1 cut(s) 2752
BarI GAAGNNNNNNTAC 4 cut(s) 720, 752, 2112, 2144
BauI CACGAG 1 cut(s) 2039
BbsI GAAGAC 1 cut(s) 2659
BbvI GCAGC 6 cut(s) 1259, 1600, 1765, 1798, 2209, 2276
BcgI CGANNNNNNTGC 2 cut(s) 1240, 1274
BciT130I CCWGG 3 cut(s) 1730, 1766, 1948
BclI TGATCA 3 cut(s) 655, 1687, 2634
BcoDI GTCTC 4 cut(s) 685, 2011, 2042, 2469
BcuI ACTAGT 2 cut(s) 578, 856
BfaI CTAG 7 cut(s) 168, 186, 339, 579, 857, 1160, 1428
BfmI CTRYAG 3 cut(s) 492, 572, 2076
BfoI RGCGCY 2 cut(s) 518, 2507
BglII AGATCT 1 cut(s) 932
BisI GCNGC 7 cut(s) 758, 1248, 1614, 1754, 1812, 2223, 2265
BlsI GCNGC 7 cut(s) 759, 1249, 1615, 1755, 1813, 2224, 2266
BmcAI AGTACT 2 cut(s) 505, 2338
Bme1390I CCNGG 3 cut(s) 1730, 1766, 1948
Bme18I GGWCC 1 cut(s) 315
BmeRI GACNNNNNGTC 1 cut(s) 1577
BmgT120I GGNCC 2 cut(s) 164, 315
BmiI GGNNCC 4 cut(s) 316, 817, 2600, 2754
BmrFI CCNGG 3 cut(s) 1730, 1766, 1948
BmrI ACTGGG 1 cut(s) 878
BmsI GCATC 4 cut(s) 348, 931, 1440, 1851
BmuI ACTGGG 1 cut(s) 878
BpiI GAAGAC 1 cut(s) 2659
BpmI CTGGAG 3 cut(s) 1362, 1695, 2628
Bpu14I TTCGAA 1 cut(s) 332
BpuEI CTTGAG 3 cut(s) 1534, 1655, 2300
BsaAI YACGTR 1 cut(s) 35
BsaBI GATNNNNATC 2 cut(s) 1454, 1486
BsaI GGTCTC 1 cut(s) 2042
BsaJI CCNNGG 4 cut(s) 244, 636, 1627, 1947
BsaWI WCCGGW 2 cut(s) 1123, 2760
BsaXI ACNNNNNCTCC 2 cut(s) 543, 573
Bsc4I CCNNNNNNNGG 2 cut(s) 1596, 1929
Bse118I RCCGGY 2 cut(s) 2095, 2760
Bse1I ACTGG 3 cut(s) 873, 2183, 2344
Bse3DI GCAATG 6 cut(s) 180, 481, 1167, 1590, 1608, 2076
Bse8I GATNNNNATC 2 cut(s) 1454, 1486
BseAI TCCGGA 1 cut(s) 1123
BseBI CCWGG 3 cut(s) 1730, 1766, 1948
BseDI CCNNGG 4 cut(s) 244, 636, 1627, 1947
BseGI GGATG 8 cut(s) 529, 670, 879, 1455, 1455, 2050, 2457, 2467
BseJI GATNNNNATC 2 cut(s) 1454, 1486
BseLI CCNNNNNNNGG 2 cut(s) 1596, 1929
BseMI GCAATG 6 cut(s) 180, 481, 1167, 1590, 1608, 2076
BseMII CTCAG 3 cut(s) 48, 912, 2145
BseNI ACTGG 3 cut(s) 873, 2183, 2344
BseRI GAGGAG 2 cut(s) 368, 1037
BseXI GCAGC 6 cut(s) 1259, 1600, 1765, 1798, 2209, 2276
BseYI CCCAGC 1 cut(s) 517
BsgI GTGCAG 4 cut(s) 1523, 1743, 2044, 2742
BshFI GGCC 7 cut(s) 166, 635, 1038, 1176, 1632, 1764, 1946
BshTI ACCGGT 1 cut(s) 2760
BsiSI CCGG 3 cut(s) 1124, 2096, 2761
BslFI GGGAC 4 cut(s) 328, 686, 1012, 2075
BslI CCNNNNNNNGG 2 cut(s) 1596, 1929
BsmAI GTCTC 4 cut(s) 685, 2011, 2042, 2469
BsmFI GGGAC 4 cut(s) 328, 686, 1012, 2075
BsmI GAATGC 3 cut(s) 240, 340, 1606
BsnI GGCC 7 cut(s) 166, 635, 1038, 1176, 1632, 1764, 1946
Bso31I GGTCTC 1 cut(s) 2042
Bsp119I TTCGAA 1 cut(s) 332
Bsp13I TCCGGA 1 cut(s) 1123
Bsp1407I TGTACA 1 cut(s) 2706
Bsp143I GATC 8 cut(s) 655, 932, 1687, 2125, 2138, 2287, 2634, 2752
Bsp19I CCATGG 2 cut(s) 244, 1627
BspACI CCGC 6 cut(s) 757, 813, 1569, 1836, 2209, 2734
BspANI GGCC 7 cut(s) 166, 635, 1038, 1176, 1632, 1764, 1946
BspCNI CTCAG 3 cut(s) 49, 913, 2146
BspEI TCCGGA 1 cut(s) 1123
BspHI TCATGA 3 cut(s) 1359, 1905, 1960
BspLI GGNNCC 4 cut(s) 316, 817, 2600, 2754
BspMAI CTGCAG 1 cut(s) 576
BspPI GGATC 3 cut(s) 2282, 2747, 2760
BspQI GCTCTTC 1 cut(s) 1663
BspT104I TTCGAA 1 cut(s) 332
BspTNI GGTCTC 1 cut(s) 2042
BsrDI GCAATG 6 cut(s) 180, 481, 1167, 1590, 1608, 2076
BsrFI RCCGGY 2 cut(s) 2095, 2760
BsrGI TGTACA 1 cut(s) 2706
BsrI ACTGG 3 cut(s) 873, 2183, 2344
BssAI RCCGGY 2 cut(s) 2095, 2760
BssECI CCNNGG 4 cut(s) 244, 636, 1627, 1947
BssMI GATC 8 cut(s) 655, 932, 1687, 2125, 2138, 2287, 2634, 2752
BssSI CACGAG 1 cut(s) 2039
BssT1I CCWWGG 2 cut(s) 244, 1627
Bst2BI CACGAG 1 cut(s) 2039
Bst2UI CCWGG 3 cut(s) 1730, 1766, 1948
Bst4CI ACNGT 5 cut(s) 679, 741, 790, 1579, 2606
Bst6I CTCTTC 1 cut(s) 1663
BstAPI GCANNNNNTGC 5 cut(s) 1109, 1169, 1267, 1601, 1610
BstAUI TGTACA 1 cut(s) 2706
BstBAI YACGTR 1 cut(s) 35
BstBI TTCGAA 1 cut(s) 332
BstC8I GCNNGC 7 cut(s) 643, 1006, 1114, 1174, 2023, 2406, 2530
BstDEI CTNAG 5 cut(s) 57, 921, 1229, 1530, 2154
BstDSI CCRYGG 2 cut(s) 244, 1627
BstF5I GGATG 8 cut(s) 529, 670, 879, 1455, 1455, 2050, 2457, 2467
BstH2I RGCGCY 2 cut(s) 518, 2507
BstHHI GCGC 2 cut(s) 517, 2506
BstKTI GATC 8 cut(s) 658, 935, 1690, 2128, 2141, 2290, 2637, 2755
BstMAI GTCTC 4 cut(s) 685, 2011, 2042, 2469
BstMBI GATC 8 cut(s) 655, 932, 1687, 2125, 2138, 2287, 2634, 2752
BstMWI GCNNNNNNNGC 9 cut(s) 454, 718, 1109, 1169, 1267, 1601, 1610, 1619, 1730
BstNI CCWGG 3 cut(s) 1730, 1766, 1948
BstNSI RCATGY 6 cut(s) 645, 764, 1008, 2229, 2412, 2717
BstSCI CCNGG 3 cut(s) 1728, 1764, 1946
BstSFI CTRYAG 3 cut(s) 492, 572, 2076
BstV1I GCAGC 6 cut(s) 1259, 1600, 1765, 1798, 2209, 2276
BstV2I GAAGAC 1 cut(s) 2659
BstX2I RGATCY 3 cut(s) 932, 2287, 2752
BstYI RGATCY 3 cut(s) 932, 2287, 2752
BsuRI GGCC 7 cut(s) 166, 635, 1038, 1176, 1632, 1764, 1946
BtgI CCRYGG 2 cut(s) 244, 1627
BtgZI GCGATG 2 cut(s) 1829, 2222
BtsCI GGATG 8 cut(s) 529, 670, 879, 1455, 1455, 2050, 2457, 2467
BtsIMutI CAGTG 2 cut(s) 2611, 2782
Cac8I GCNNGC 7 cut(s) 643, 1006, 1114, 1174, 2023, 2406, 2530
CciI TCATGA 3 cut(s) 1359, 1905, 1960
CfoI GCGC 2 cut(s) 517, 2506
Cfr10I RCCGGY 2 cut(s) 2095, 2760
Cfr13I GGNCC 2 cut(s) 164, 315
CseI GACGC 1 cut(s) 1246
Csp6I GTAC 5 cut(s) 4, 291, 504, 2337, 2707
CspAI ACCGGT 1 cut(s) 2760
CviQI GTAC 5 cut(s) 4, 291, 504, 2337, 2707
DdeI CTNAG 5 cut(s) 57, 921, 1229, 1530, 2154
DpnI GATC 8 cut(s) 657, 934, 1689, 2127, 2140, 2289, 2636, 2754
DpnII GATC 8 cut(s) 655, 932, 1687, 2125, 2138, 2287, 2634, 2752
DraI TTTAAA 1 cut(s) 1354
DriI GACNNNNNGTC 1 cut(s) 1577
EaeI YGGCCR 1 cut(s) 633
Eam1104I CTCTTC 1 cut(s) 1663
Eam1105I GACNNNNNGTC 1 cut(s) 1577
EarI CTCTTC 1 cut(s) 1663
EciI GGCGGA 2 cut(s) 2224, 2749
Eco130I CCWWGG 2 cut(s) 244, 1627
Eco147I AGGCCT 1 cut(s) 1176
Eco31I GGTCTC 1 cut(s) 2042
Eco32I GATATC 1 cut(s) 1911
Eco47I GGWCC 1 cut(s) 315
Eco47III AGCGCT 1 cut(s) 2505
Eco57I CTGAAG 3 cut(s) 851, 957, 1877
EcoO109I RGGNCCY 1 cut(s) 315
EcoRI GAATTC 4 cut(s) 688, 778, 846, 1294
EcoRII CCWGG 3 cut(s) 1728, 1764, 1946
EcoRV GATATC 1 cut(s) 1911
EcoT14I CCWWGG 2 cut(s) 244, 1627
EcoT22I ATGCAT 2 cut(s) 1207, 1465
ErhI CCWWGG 2 cut(s) 244, 1627
FaqI GGGAC 4 cut(s) 328, 686, 1012, 2075
FauI CCCGC 1 cut(s) 1829
FauNDI CATATG 1 cut(s) 660
FbaI TGATCA 3 cut(s) 655, 1687, 2634
Fnu4HI GCNGC 7 cut(s) 758, 1248, 1614, 1754, 1812, 2223, 2265
FokI GGATG 8 cut(s) 536, 677, 886, 1442, 1462, 2057, 2454, 2464
Fsp4HI GCNGC 7 cut(s) 758, 1248, 1614, 1754, 1812, 2223, 2265
FspBI CTAG 7 cut(s) 168, 186, 339, 579, 857, 1160, 1428
GlaI GCGC 2 cut(s) 516, 2505
GluI GCNGC 7 cut(s) 758, 1248, 1614, 1754, 1812, 2223, 2265
GsaI CCCAGC 1 cut(s) 521
GsuI CTGGAG 3 cut(s) 1362, 1695, 2628
HaeII RGCGCY 2 cut(s) 518, 2507
HaeIII GGCC 7 cut(s) 166, 635, 1038, 1176, 1632, 1764, 1946
HapII CCGG 3 cut(s) 1124, 2096, 2761
HgaI GACGC 1 cut(s) 1246
HhaI GCGC 2 cut(s) 517, 2506
Hin6I GCGC 2 cut(s) 515, 2504
HinP1I GCGC 2 cut(s) 515, 2504
HincII GTYRAC 1 cut(s) 539
HindII GTYRAC 1 cut(s) 539
HindIII AAGCTT 4 cut(s) 69, 1226, 1641, 1775
HpaII CCGG 3 cut(s) 1124, 2096, 2761
Hpy166II GTNNAC 4 cut(s) 539, 1681, 2654, 2707
Hpy8I GTNNAC 4 cut(s) 539, 1681, 2654, 2707
Hpy99I CGWCG 1 cut(s) 799
HpyCH4III ACNGT 5 cut(s) 679, 741, 790, 1579, 2606
HpyCH4IV ACGT 3 cut(s) 19, 34, 797
HpyF10VI GCNNNNNNNGC 9 cut(s) 454, 718, 1109, 1169, 1267, 1601, 1610, 1619, 1730
HpyF3I CTNAG 5 cut(s) 57, 921, 1229, 1530, 2154
HpySE526I ACGT 3 cut(s) 19, 34, 797
HspAI GCGC 2 cut(s) 515, 2504
Kpn2I TCCGGA 1 cut(s) 1123
Ksp22I TGATCA 3 cut(s) 655, 1687, 2634
Kzo9I GATC 8 cut(s) 655, 932, 1687, 2125, 2138, 2287, 2634, 2752
LguI GCTCTTC 1 cut(s) 1663
LmnI GCTCC 3 cut(s) 2211, 2501, 2584
Lsp1109I GCAGC 6 cut(s) 1259, 1600, 1765, 1798, 2209, 2276
LweI GCATC 4 cut(s) 348, 931, 1440, 1851
MaeI CTAG 7 cut(s) 168, 186, 339, 579, 857, 1160, 1428
MaeII ACGT 3 cut(s) 19, 34, 797
MaeIII GTNAC 8 cut(s) 193, 376, 1087, 1210, 1285, 1884, 1996, 2185
MalI GATC 8 cut(s) 657, 934, 1689, 2127, 2140, 2289, 2636, 2754
MboI GATC 8 cut(s) 655, 932, 1687, 2125, 2138, 2287, 2634, 2752
MflI RGATCY 3 cut(s) 932, 2287, 2752
MlyI GAGTC 4 cut(s) 116, 816, 1034, 2691
MmeI TCCRAC 2 cut(s) 144, 1177
Mph1103I ATGCAT 2 cut(s) 1207, 1465
MroI TCCGGA 1 cut(s) 1123
MroXI GAANNNNTTC 3 cut(s) 1059, 1851, 2149
MseI TTAA 8 cut(s) 765, 908, 1094, 1353, 1401, 1773, 2172, 2661
MslI CAYNNNNRTG 6 cut(s) 86, 309, 608, 663, 2718, 2775
MspI CCGG 3 cut(s) 1124, 2096, 2761
MspR9I CCNGG 3 cut(s) 1730, 1766, 1948
Mva1269I GAATGC 3 cut(s) 240, 340, 1606
MvaI CCWGG 3 cut(s) 1730, 1766, 1948
MwoI GCNNNNNNNGC 9 cut(s) 454, 718, 1109, 1169, 1267, 1601, 1610, 1619, 1730
NcoI CCATGG 2 cut(s) 244, 1627
NdeI CATATG 1 cut(s) 660
NdeII GATC 8 cut(s) 655, 932, 1687, 2125, 2138, 2287, 2634, 2752
NlaIV GGNNCC 4 cut(s) 316, 817, 2600, 2754
NmeAIII GCCGAG 1 cut(s) 661
NmuCI GTSAC 4 cut(s) 1087, 1884, 1996, 2185
NsiI ATGCAT 2 cut(s) 1207, 1465
NspI RCATGY 6 cut(s) 645, 764, 1008, 2229, 2412, 2717
NspV TTCGAA 1 cut(s) 332
PaeI GCATGC 2 cut(s) 645, 1008
PagI TCATGA 3 cut(s) 1359, 1905, 1960
PceI AGGCCT 1 cut(s) 1176
PciSI GCTCTTC 1 cut(s) 1663
PcsI WCGNNNNNNNCGW 1 cut(s) 89
PctI GAATGC 3 cut(s) 240, 340, 1606
PdmI GAANNNNTTC 3 cut(s) 1059, 1851, 2149
PfeI GAWTC 7 cut(s) 731, 878, 1147, 1423, 1487, 2300, 2674
PflFI GACNNNGTC 2 cut(s) 677, 1994
PflMI CCANNNNNTGG 1 cut(s) 1596
PinAI ACCGGT 1 cut(s) 2760
PkrI GCNGC 7 cut(s) 759, 1249, 1615, 1755, 1813, 2224, 2266
PleI GAGTC 4 cut(s) 116, 815, 1033, 2690
PpsI GAGTC 4 cut(s) 116, 815, 1033, 2690
Ppu21I YACGTR 1 cut(s) 35
PpuMI RGGWCCY 1 cut(s) 315
PshBI ATTAAT 1 cut(s) 1401
Psp5II RGGWCCY 1 cut(s) 315
Psp6I CCWGG 3 cut(s) 1728, 1764, 1946
PspFI CCCAGC 1 cut(s) 517
PspGI CCWGG 3 cut(s) 1728, 1764, 1946
PspN4I GGNNCC 4 cut(s) 316, 817, 2600, 2754
PspPI GGNCC 2 cut(s) 164, 315
PspPPI RGGWCCY 1 cut(s) 315
PstI CTGCAG 1 cut(s) 576
PsuI RGATCY 3 cut(s) 932, 2287, 2752
PsyI GACNNNGTC 2 cut(s) 677, 1994
RsaI GTAC 5 cut(s) 5, 292, 505, 2338, 2708
RsaNI GTAC 5 cut(s) 4, 291, 504, 2337, 2707
RseI CAYNNNNRTG 6 cut(s) 86, 309, 608, 663, 2718, 2775
SapI GCTCTTC 1 cut(s) 1663
SaqAI TTAA 8 cut(s) 765, 908, 1094, 1353, 1401, 1773, 2172, 2661
SatI GCNGC 7 cut(s) 758, 1248, 1614, 1754, 1812, 2223, 2265
Sau3AI GATC 8 cut(s) 655, 932, 1687, 2125, 2138, 2287, 2634, 2752
Sau96I GGNCC 2 cut(s) 164, 315
ScaI AGTACT 2 cut(s) 505, 2338
SchI GAGTC 4 cut(s) 116, 816, 1034, 2691
ScrFI CCNGG 3 cut(s) 1730, 1766, 1948
SfaNI GCATC 4 cut(s) 348, 931, 1440, 1851
SfcI CTRYAG 3 cut(s) 492, 572, 2076
SfuI TTCGAA 1 cut(s) 332
SinI GGWCC 1 cut(s) 315
SmiMI CAYNNNNRTG 6 cut(s) 86, 309, 608, 663, 2718, 2775
SmlI CTYRAG 3 cut(s) 1549, 1634, 2279
SmoI CTYRAG 3 cut(s) 1549, 1634, 2279
SpeI ACTAGT 2 cut(s) 578, 856
SphI GCATGC 2 cut(s) 645, 1008
SseBI AGGCCT 1 cut(s) 1176
SsiI CCGC 6 cut(s) 757, 813, 1569, 1836, 2209, 2734
SspMI CTAG 7 cut(s) 168, 186, 339, 579, 857, 1160, 1428
StuI AGGCCT 1 cut(s) 1176
StyD4I CCNGG 3 cut(s) 1728, 1764, 1946
StyI CCWWGG 2 cut(s) 244, 1627
TaaI ACNGT 5 cut(s) 679, 741, 790, 1579, 2606
TaiI ACGT 3 cut(s) 22, 37, 800
TaqI TCGA 9 cut(s) 8, 24, 92, 332, 692, 794, 1938, 2384, 2680
TatI WGTACW 4 cut(s) 3, 503, 2336, 2706
TauI GCSGC 1 cut(s) 760
TfiI GAWTC 7 cut(s) 731, 878, 1147, 1423, 1487, 2300, 2674
Tru1I TTAA 8 cut(s) 765, 908, 1094, 1353, 1401, 1773, 2172, 2661
Tru9I TTAA 8 cut(s) 765, 908, 1094, 1353, 1401, 1773, 2172, 2661
TscAI CASTG 2 cut(s) 2611, 2782
TseFI GTSAC 4 cut(s) 1087, 1884, 1996, 2185
TseI GCWGC 6 cut(s) 1247, 1613, 1753, 1811, 2222, 2264
Tsp45I GTSAC 4 cut(s) 1087, 1884, 1996, 2185
TspRI CASTG 2 cut(s) 2611, 2782
Tth111I GACNNNGTC 2 cut(s) 677, 1994
Van91I CCANNNNNTGG 1 cut(s) 1596
VpaK11BI GGWCC 1 cut(s) 315
VspI ATTAAT 1 cut(s) 1401
XceI RCATGY 6 cut(s) 645, 764, 1008, 2229, 2412, 2717
XcmI CCANNNNNNNNNTGG 2 cut(s) 2412, 2777
XmnI GAANNNNTTC 3 cut(s) 1059, 1851, 2149
XspI CTAG 7 cut(s) 168, 186, 339, 579, 857, 1160, 1428
ZrmI AGTACT 2 cut(s) 505, 2338
Zsp2I ATGCAT 2 cut(s) 1207, 1465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.