Rh5DG177500

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0013464
Physical Location & Seq
Forward (+)
19599937 .. 19602603
2667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG177500.1

Sequence Viewer

Length: 2160 bp
ATGGTTGGCTGTGGTGTTGGGCCTAGTGGGTTTGTCATTGCTAGTTTGGTTACTGCGTGTGATAAGTCTGGTTCTATGTTTTGTGAAGGAATGCAAGTCCATGGCTTTGTGGTGAAGATTGGGTTGTTGTGTGATGTGTTTGTGGGTACTTCTGTTTTGCATTTTTATGGGACCTATGGTGTTGTTTCGAATGCTAGAAAGGTGTTTGAGGAGATGCCAGATAAGAATGTGGTTACTTGGACTTCTTTGATTGTTGGGTATTCAAATGGTGGAGATTTGGGAGAGGTAATGAGTATATATAAGCGTATGAGGCGTGAAGGAGTGTGTTGCAATGATAACACTTTGGCTATAGTGTTTAGTACTTGTGGGGCGCTGGGGGATGAGTTATTGGGTCAACAAGTTCTTGGAGTTGCTGTGAAACTTGGACTGCAGACTAGTGTTTCTGTGGCAAATTCGCTCATATCCATGTATGGTAGCTTTGGTAATGTGGCCGAGGCATGCTATGTATTTGATCATATGGATGAAAGGGACACAGTCTCATGGAATTCGATTATTTCTGCTAATGTGCAAAATGGGCTTTGTGAAGAATCATTACGGTATTTTCATCGTATGCGGCATGTTAATAAAGAAGTGAATTCTATAACAGTTTCGACGTTGTTGGGAGTCTGCGGTTCCACAGATAAACTGAAGTGGGGCAGGGGAATTCATGGACTAGTGGTGAAACTGGGGATGGAATCAAATGTTTGTGTTGGCAATACTCTTTTAAGTATGTATTCTGAGGCTGGAAGATCTGAAGATGCTGAATTAGTTTTCAAAAGAATGATAGAGAAGGATATAATCTCATGGAACTCCATGTTAGCATGCTATGTTCAGAATGAGGAGTCCCAAAATGGCCTCAAACATTTTGCTGAAATGCTTCGGACAAGAAAACCAATAAATTATGTGACTTTAACAAGTGCATTGTCTGCCTGCTCAAATCCGGAATTTGTAGTTGAAGGAAAGATTCTTCATGCTCTAGCAGTCATTGCAGGCCTACAAGACAATCTGATAGTCGGAAATGCATTAGTTACCATGTATGGAAAGCTTAGTAAGACGCTTGAAGCAGCGAAGGTGTTGCAAACAATGCCCAAGCGAGATGAAGTTACTTGGAATTCTCTCATCGGTAGTTATGCTGAAAACAAAGAACCAGATGAGGTAATTACAGCTTTTAAACTCATGAGAAAAGAAGGAACTCCAGCAAACTACATTACAATAATTAATGTTCTCGGTGCTTTCCTGATTCCTAGTGATTTACTAAAACATGGGATGCCCATCCATGCATACATAGTTCTAACAGGATTTGAATCAGAAAAGTATGTGCAGAGTTCCCTTATTACGATGTATGCTAAGTGTGGTGATTTGAACTCAAGTAATTCCATATTTAGCGGACTAACTGTCAAGAACTCCATTGCATGGAATGCAATCATTGCTGCAAATGCTAACCATGGCCTTGAGGAAGCTTTGAAACTTGCTTTGATGATGAGAAGAGCTGGAGTGGACTTTGATCAGTTCAGCTTATCTATCGCACTTTCTGTTAGTGCAGACCTGGCTATGTTGGTAGAAGGTCAGCAGCTTCATGGCCTGGTAGTTAAGCTTGGTTTTGACACAGACCATTATATTACAAATGCTGCGATGGATATGTATGGAAAATGCGGGGAGATGGAAGATGTTCTGAAGATGCTTCCCTCACCAACCATTAGGTCACGATTATCATGGAACATCATGATATCATCGTTTGCCAAACATGGGTGTTTCGAGAAGGCCAGGGAGAGCTTTCATGAAATGACACAGCAGATTTATGCCAAGTTGGGAGCGCTTATGAAGATGATTAGAGAAGCAGGCTATGTTCCTGATACAAGCTACTCCCTGCAAGATACCGATGAAGAACAAAAGGAGCAAAATCTTTGGAACCACAGTGAGAGAATTGCCCTTGCTTTTGGGTTGATCAATACTCCAGAAGGTTCACCTGTTAAGGTCTTCAAGAATCTTCGAGTCTGTGGTGATTGCCATTCTGTGTACAAGCATGTTAGTGCAGTAGTTGGGCGGAAAATCATATTGAGGGATCCATACCGGTTTCACCATTTCAGTGATGGCAAGTGTTCTTGTTCTGACTATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

719

Amino Acids

79.18

Weight (kDa)

6.22

Isoelectric Point (pI)

30.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 75 - 122 4.6e-08 PPR repeat family
PPR PF01535 78 - 108 7.7e-07 PPR repeat
PPR_2 PF13041 176 - 223 9.4e-06 PPR repeat family
PPR_2 PF13041 379 - 418 1e-05 PPR repeat family
PPR PF01535 381 - 410 7.5e-06 PPR repeat
PPR PF01535 584 - 609 2.4e-06 PPR repeat
DYW_deaminase PF14432 627 - 719 1.3e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013464)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16480 AT1G16480
fragaria_vesca FvH4_3g15200
malus_domestica MD10G1206400.v1.1
prunus_persica Prupe.4G135900_v2.0.a1
pyrus_communis pycom10g17620
rosa_chinensis RchiOBHm_Chr5g0025641
rosa_laevigata RLG00000032854
rosa_multiflora Rmu_sc0002137.1_g000002 Rmu_sc0014484.1_g000002
rosa_roxburghii Rroxscaffold_1G00053530 Rroxscaffold_1G00054190
rosa_rugosa Rorug05G0087400
rosa_samantha Rh5AG179200 Rh5BG178300 Rh5CG195900 Rh5DG177500
rosa_wichuraiana Rw5G016270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1452
AccIII TCCGGA 1 cut(s) 979
AciI CCGC 5 cut(s) 613, 669, 1425, 1692, 2083
AclWI GGATC 2 cut(s) 2096, 2109
AcoI YGGCCR 1 cut(s) 489
AcsI RAATTY 6 cut(s) 451, 544, 634, 702, 983, 1150
AcuI CTGAAG 3 cut(s) 707, 813, 1733
AfaI GTAC 3 cut(s) 148, 361, 2057
AfeI AGCGCT 1 cut(s) 1854
AfiI CCNNNNNNNGG 2 cut(s) 1452, 1785
AgeI ACCGGT 1 cut(s) 2109
AgsI TTSAA 8 cut(s) 264, 814, 995, 1100, 1343, 1402, 1504, 2020
AhdI GACNNNNNGTC 1 cut(s) 1433
AhlI ACTAGT 2 cut(s) 434, 712
AjnI CCWGG 3 cut(s) 1584, 1620, 1802
Alw26I GTCTC 1 cut(s) 541
AlwI GGATC 2 cut(s) 2096, 2109
Aor13HI TCCGGA 1 cut(s) 979
Aor51HI AGCGCT 1 cut(s) 1854
AoxI GGCC 7 cut(s) 20, 489, 892, 1030, 1486, 1618, 1800
ApeKI GCWGC 4 cut(s) 1103, 1469, 1609, 1667
ApoI RAATTY 6 cut(s) 451, 544, 634, 702, 983, 1150
AseI ATTAAT 1 cut(s) 1257
AsiGI ACCGGT 1 cut(s) 2109
Asp700I GAANNNNTTC 2 cut(s) 915, 1707
AspLEI GCGC 2 cut(s) 373, 1855
AspS9I GGNCC 2 cut(s) 20, 171
AsuHPI GGTGA 7 cut(s) 124, 730, 1406, 1719, 1995, 2051, 2108
AsuII TTCGAA 1 cut(s) 188
AvaII GGWCC 1 cut(s) 171
BaeI ACNNNNGTAYC 2 cut(s) 138, 171
BamHI GGATCC 1 cut(s) 2101
BarI GAAGNNNNNNTAC 2 cut(s) 576, 608
BbsI GAAGAC 1 cut(s) 2008
BbvI GCAGC 4 cut(s) 1115, 1456, 1621, 1654
BccI CCATC 5 cut(s) 724, 1319, 1666, 1693, 2123
BcgI CGANNNNNNTGC 2 cut(s) 1096, 1130
BciT130I CCWGG 3 cut(s) 1586, 1622, 1804
BclI TGATCA 3 cut(s) 511, 1543, 1983
BcoDI GTCTC 1 cut(s) 541
BcuI ACTAGT 2 cut(s) 434, 712
BfaI CTAG 7 cut(s) 24, 42, 195, 435, 713, 1016, 1284
BfmI CTRYAG 2 cut(s) 348, 428
BfoI RGCGCY 2 cut(s) 374, 1856
BglII AGATCT 1 cut(s) 788
BisI GCNGC 5 cut(s) 614, 1104, 1470, 1610, 1668
BlsI GCNGC 5 cut(s) 615, 1105, 1471, 1611, 1669
BmcAI AGTACT 1 cut(s) 361
Bme1390I CCNGG 3 cut(s) 1586, 1622, 1804
Bme18I GGWCC 1 cut(s) 171
BmeRI GACNNNNNGTC 1 cut(s) 1433
BmgT120I GGNCC 2 cut(s) 20, 171
BmiI GGNNCC 4 cut(s) 172, 673, 1949, 2103
BmrFI CCNGG 3 cut(s) 1586, 1622, 1804
BmrI ACTGGG 1 cut(s) 734
BmsI GCATC 4 cut(s) 204, 787, 1296, 1707
BmuI ACTGGG 1 cut(s) 734
BpiI GAAGAC 1 cut(s) 2008
BpmI CTGGAG 3 cut(s) 1218, 1551, 1977
Bpu14I TTCGAA 1 cut(s) 188
BpuEI CTTGAG 2 cut(s) 1390, 1511
BsaBI GATNNNNATC 2 cut(s) 1310, 1342
BsaJI CCNNGG 4 cut(s) 100, 492, 1483, 1803
BsaWI WCCGGW 2 cut(s) 979, 2109
BsaXI ACNNNNNCTCC 2 cut(s) 399, 429
Bsc4I CCNNNNNNNGG 2 cut(s) 1452, 1785
Bse118I RCCGGY 1 cut(s) 2109
Bse1I ACTGG 1 cut(s) 729
Bse3DI GCAATG 5 cut(s) 36, 337, 1023, 1446, 1464
Bse8I GATNNNNATC 2 cut(s) 1310, 1342
BseAI TCCGGA 1 cut(s) 979
BseBI CCWGG 3 cut(s) 1586, 1622, 1804
BseDI CCNNGG 4 cut(s) 100, 492, 1483, 1803
BseGI GGATG 5 cut(s) 385, 526, 735, 1311, 1311
BseJI GATNNNNATC 2 cut(s) 1310, 1342
BseLI CCNNNNNNNGG 2 cut(s) 1452, 1785
BseMI GCAATG 5 cut(s) 36, 337, 1023, 1446, 1464
BseMII CTCAG 1 cut(s) 768
BseNI ACTGG 1 cut(s) 729
BseRI GAGGAG 2 cut(s) 224, 893
BseXI GCAGC 4 cut(s) 1115, 1456, 1621, 1654
BseYI CCCAGC 1 cut(s) 373
BsgI GTGCAG 3 cut(s) 1379, 1599, 2091
BshFI GGCC 7 cut(s) 22, 491, 894, 1032, 1488, 1620, 1802
BshTI ACCGGT 1 cut(s) 2109
BsiSI CCGG 2 cut(s) 980, 2110
BslFI GGGAC 3 cut(s) 184, 542, 868
BslI CCNNNNNNNGG 2 cut(s) 1452, 1785
BsmAI GTCTC 1 cut(s) 541
BsmFI GGGAC 3 cut(s) 184, 542, 868
BsmI GAATGC 3 cut(s) 96, 196, 1462
BsnI GGCC 7 cut(s) 22, 491, 894, 1032, 1488, 1620, 1802
Bsp119I TTCGAA 1 cut(s) 188
Bsp13I TCCGGA 1 cut(s) 979
Bsp1407I TGTACA 1 cut(s) 2055
Bsp143I GATC 5 cut(s) 511, 788, 1543, 1983, 2101
Bsp19I CCATGG 2 cut(s) 100, 1483
BspACI CCGC 5 cut(s) 613, 669, 1425, 1692, 2083
BspANI GGCC 7 cut(s) 22, 491, 894, 1032, 1488, 1620, 1802
BspCNI CTCAG 1 cut(s) 769
BspEI TCCGGA 1 cut(s) 979
BspHI TCATGA 3 cut(s) 1215, 1761, 1816
BspLI GGNNCC 4 cut(s) 172, 673, 1949, 2103
BspMAI CTGCAG 1 cut(s) 432
BspPI GGATC 2 cut(s) 2096, 2109
BspQI GCTCTTC 1 cut(s) 1519
BspT104I TTCGAA 1 cut(s) 188
BsrDI GCAATG 5 cut(s) 36, 337, 1023, 1446, 1464
BsrFI RCCGGY 1 cut(s) 2109
BsrGI TGTACA 1 cut(s) 2055
BsrI ACTGG 1 cut(s) 729
BssAI RCCGGY 1 cut(s) 2109
BssECI CCNNGG 4 cut(s) 100, 492, 1483, 1803
BssMI GATC 5 cut(s) 511, 788, 1543, 1983, 2101
BssT1I CCWWGG 2 cut(s) 100, 1483
Bst2UI CCWGG 3 cut(s) 1586, 1622, 1804
Bst4CI ACNGT 5 cut(s) 535, 597, 646, 1435, 1955
Bst6I CTCTTC 1 cut(s) 1519
BstAPI GCANNNNNTGC 5 cut(s) 965, 1025, 1123, 1457, 1466
BstAUI TGTACA 1 cut(s) 2055
BstBI TTCGAA 1 cut(s) 188
BstC8I GCNNGC 5 cut(s) 499, 862, 970, 1030, 1879
BstDEI CTNAG 3 cut(s) 777, 1085, 1386
BstDSI CCRYGG 2 cut(s) 100, 1483
BstF5I GGATG 5 cut(s) 385, 526, 735, 1311, 1311
BstH2I RGCGCY 2 cut(s) 374, 1856
BstHHI GCGC 2 cut(s) 373, 1855
BstKTI GATC 5 cut(s) 514, 791, 1546, 1986, 2104
BstMAI GTCTC 1 cut(s) 541
BstMBI GATC 5 cut(s) 511, 788, 1543, 1983, 2101
BstMWI GCNNNNNNNGC 9 cut(s) 310, 574, 965, 1025, 1123, 1457, 1466, 1475, 1586
BstNI CCWGG 3 cut(s) 1586, 1622, 1804
BstNSI RCATGY 4 cut(s) 501, 620, 864, 2066
BstSCI CCNGG 3 cut(s) 1584, 1620, 1802
BstSFI CTRYAG 2 cut(s) 348, 428
BstV1I GCAGC 4 cut(s) 1115, 1456, 1621, 1654
BstV2I GAAGAC 1 cut(s) 2008
BstX2I RGATCY 2 cut(s) 788, 2101
BstYI RGATCY 2 cut(s) 788, 2101
BsuRI GGCC 7 cut(s) 22, 491, 894, 1032, 1488, 1620, 1802
BtgI CCRYGG 2 cut(s) 100, 1483
BtgZI GCGATG 1 cut(s) 1685
BtsCI GGATG 5 cut(s) 385, 526, 735, 1311, 1311
BtsIMutI CAGTG 2 cut(s) 1960, 2131
Cac8I GCNNGC 5 cut(s) 499, 862, 970, 1030, 1879
CciI TCATGA 3 cut(s) 1215, 1761, 1816
CfoI GCGC 2 cut(s) 373, 1855
Cfr10I RCCGGY 1 cut(s) 2109
Cfr13I GGNCC 2 cut(s) 20, 171
CseI GACGC 1 cut(s) 1102
Csp6I GTAC 3 cut(s) 147, 360, 2056
CspAI ACCGGT 1 cut(s) 2109
CviQI GTAC 3 cut(s) 147, 360, 2056
DdeI CTNAG 3 cut(s) 777, 1085, 1386
DpnI GATC 5 cut(s) 513, 790, 1545, 1985, 2103
DpnII GATC 5 cut(s) 511, 788, 1543, 1983, 2101
DraI TTTAAA 1 cut(s) 1210
DriI GACNNNNNGTC 1 cut(s) 1433
EaeI YGGCCR 1 cut(s) 489
Eam1104I CTCTTC 1 cut(s) 1519
Eam1105I GACNNNNNGTC 1 cut(s) 1433
EarI CTCTTC 1 cut(s) 1519
EciI GGCGGA 1 cut(s) 2098
Eco130I CCWWGG 2 cut(s) 100, 1483
Eco147I AGGCCT 1 cut(s) 1032
Eco32I GATATC 1 cut(s) 1767
Eco47I GGWCC 1 cut(s) 171
Eco47III AGCGCT 1 cut(s) 1854
Eco57I CTGAAG 3 cut(s) 707, 813, 1733
EcoO109I RGGNCCY 1 cut(s) 171
EcoRI GAATTC 4 cut(s) 544, 634, 702, 1150
EcoRII CCWGG 3 cut(s) 1584, 1620, 1802
EcoRV GATATC 1 cut(s) 1767
EcoT14I CCWWGG 2 cut(s) 100, 1483
EcoT22I ATGCAT 2 cut(s) 1063, 1321
ErhI CCWWGG 2 cut(s) 100, 1483
FaqI GGGAC 3 cut(s) 184, 542, 868
FauI CCCGC 1 cut(s) 1685
FauNDI CATATG 1 cut(s) 516
FbaI TGATCA 3 cut(s) 511, 1543, 1983
Fnu4HI GCNGC 5 cut(s) 614, 1104, 1470, 1610, 1668
FokI GGATG 5 cut(s) 392, 533, 742, 1298, 1318
Fsp4HI GCNGC 5 cut(s) 614, 1104, 1470, 1610, 1668
FspBI CTAG 7 cut(s) 24, 42, 195, 435, 713, 1016, 1284
GlaI GCGC 2 cut(s) 372, 1854
GluI GCNGC 5 cut(s) 614, 1104, 1470, 1610, 1668
GsaI CCCAGC 1 cut(s) 377
GsuI CTGGAG 3 cut(s) 1218, 1551, 1977
HaeII RGCGCY 2 cut(s) 374, 1856
HaeIII GGCC 7 cut(s) 22, 491, 894, 1032, 1488, 1620, 1802
HapII CCGG 2 cut(s) 980, 2110
HgaI GACGC 1 cut(s) 1102
HhaI GCGC 2 cut(s) 373, 1855
Hin6I GCGC 2 cut(s) 371, 1853
HinP1I GCGC 2 cut(s) 371, 1853
HincII GTYRAC 1 cut(s) 395
HindII GTYRAC 1 cut(s) 395
HindIII AAGCTT 3 cut(s) 1082, 1497, 1631
HinfI GANTC 9 cut(s) 587, 663, 734, 881, 1003, 1279, 1343, 2023, 2031
HpaII CCGG 2 cut(s) 980, 2110
HphI GGTGA 7 cut(s) 124, 730, 1406, 1719, 1995, 2051, 2108
Hpy166II GTNNAC 4 cut(s) 395, 1537, 2003, 2056
Hpy188I TCNGA 9 cut(s) 778, 793, 873, 921, 1047, 1055, 1348, 1713, 2149
Hpy8I GTNNAC 4 cut(s) 395, 1537, 2003, 2056
Hpy99I CGWCG 1 cut(s) 655
HpyAV CCTTC 9 cut(s) 80, 311, 823, 989, 1102, 1220, 1595, 1792, 1991
HpyCH4III ACNGT 5 cut(s) 535, 597, 646, 1435, 1955
HpyCH4IV ACGT 1 cut(s) 653
HpyF10VI GCNNNNNNNGC 9 cut(s) 310, 574, 965, 1025, 1123, 1457, 1466, 1475, 1586
HpyF3I CTNAG 3 cut(s) 777, 1085, 1386
HpySE526I ACGT 1 cut(s) 653
HspAI GCGC 2 cut(s) 371, 1853
Kpn2I TCCGGA 1 cut(s) 979
Ksp22I TGATCA 3 cut(s) 511, 1543, 1983
Kzo9I GATC 5 cut(s) 511, 788, 1543, 1983, 2101
LguI GCTCTTC 1 cut(s) 1519
LmnI GCTCC 2 cut(s) 1850, 1933
Lsp1109I GCAGC 4 cut(s) 1115, 1456, 1621, 1654
LweI GCATC 4 cut(s) 204, 787, 1296, 1707
MaeI CTAG 7 cut(s) 24, 42, 195, 435, 713, 1016, 1284
MaeII ACGT 1 cut(s) 653
MaeIII GTNAC 6 cut(s) 49, 232, 943, 1066, 1141, 1740
MalI GATC 5 cut(s) 513, 790, 1545, 1985, 2103
MboI GATC 5 cut(s) 511, 788, 1543, 1983, 2101
MflI RGATCY 2 cut(s) 788, 2101
MlyI GAGTC 3 cut(s) 672, 890, 2040
MmeI TCCRAC 1 cut(s) 1033
Mph1103I ATGCAT 2 cut(s) 1063, 1321
MroI TCCGGA 1 cut(s) 979
MroXI GAANNNNTTC 2 cut(s) 915, 1707
MseI TTAA 7 cut(s) 621, 764, 950, 1209, 1257, 1629, 2010
MslI CAYNNNNRTG 5 cut(s) 165, 464, 519, 2067, 2124
MspI CCGG 2 cut(s) 980, 2110
MspR9I CCNGG 3 cut(s) 1586, 1622, 1804
Mva1269I GAATGC 3 cut(s) 96, 196, 1462
MvaI CCWGG 3 cut(s) 1586, 1622, 1804
MwoI GCNNNNNNNGC 9 cut(s) 310, 574, 965, 1025, 1123, 1457, 1466, 1475, 1586
NcoI CCATGG 2 cut(s) 100, 1483
NdeI CATATG 1 cut(s) 516
NdeII GATC 5 cut(s) 511, 788, 1543, 1983, 2101
NlaIV GGNNCC 4 cut(s) 172, 673, 1949, 2103
NmeAIII GCCGAG 1 cut(s) 517
NmuCI GTSAC 2 cut(s) 943, 1740
NsiI ATGCAT 2 cut(s) 1063, 1321
NspI RCATGY 4 cut(s) 501, 620, 864, 2066
NspV TTCGAA 1 cut(s) 188
PaeI GCATGC 2 cut(s) 501, 864
PagI TCATGA 3 cut(s) 1215, 1761, 1816
PceI AGGCCT 1 cut(s) 1032
PciSI GCTCTTC 1 cut(s) 1519
PctI GAATGC 3 cut(s) 96, 196, 1462
PdmI GAANNNNTTC 2 cut(s) 915, 1707
PfeI GAWTC 6 cut(s) 587, 734, 1003, 1279, 1343, 2023
PflFI GACNNNGTC 1 cut(s) 533
PflMI CCANNNNNTGG 1 cut(s) 1452
PinAI ACCGGT 1 cut(s) 2109
PkrI GCNGC 5 cut(s) 615, 1105, 1471, 1611, 1669
PleI GAGTC 3 cut(s) 671, 889, 2039
PpsI GAGTC 3 cut(s) 671, 889, 2039
PpuMI RGGWCCY 1 cut(s) 171
PshBI ATTAAT 1 cut(s) 1257
Psp5II RGGWCCY 1 cut(s) 171
Psp6I CCWGG 3 cut(s) 1584, 1620, 1802
PspFI CCCAGC 1 cut(s) 373
PspGI CCWGG 3 cut(s) 1584, 1620, 1802
PspN4I GGNNCC 4 cut(s) 172, 673, 1949, 2103
PspPI GGNCC 2 cut(s) 20, 171
PspPPI RGGWCCY 1 cut(s) 171
PstI CTGCAG 1 cut(s) 432
PsuI RGATCY 2 cut(s) 788, 2101
PsyI GACNNNGTC 1 cut(s) 533
RsaI GTAC 3 cut(s) 148, 361, 2057
RsaNI GTAC 3 cut(s) 147, 360, 2056
RseI CAYNNNNRTG 5 cut(s) 165, 464, 519, 2067, 2124
SapI GCTCTTC 1 cut(s) 1519
SaqAI TTAA 7 cut(s) 621, 764, 950, 1209, 1257, 1629, 2010
SatI GCNGC 5 cut(s) 614, 1104, 1470, 1610, 1668
Sau3AI GATC 5 cut(s) 511, 788, 1543, 1983, 2101
Sau96I GGNCC 2 cut(s) 20, 171
ScaI AGTACT 1 cut(s) 361
SchI GAGTC 3 cut(s) 672, 890, 2040
ScrFI CCNGG 3 cut(s) 1586, 1622, 1804
SfaNI GCATC 4 cut(s) 204, 787, 1296, 1707
SfcI CTRYAG 2 cut(s) 348, 428
SfuI TTCGAA 1 cut(s) 188
SinI GGWCC 1 cut(s) 171
SmiMI CAYNNNNRTG 5 cut(s) 165, 464, 519, 2067, 2124
SmlI CTYRAG 2 cut(s) 1405, 1490
SmoI CTYRAG 2 cut(s) 1405, 1490
SpeI ACTAGT 2 cut(s) 434, 712
SphI GCATGC 2 cut(s) 501, 864
SseBI AGGCCT 1 cut(s) 1032
SsiI CCGC 5 cut(s) 613, 669, 1425, 1692, 2083
SspMI CTAG 7 cut(s) 24, 42, 195, 435, 713, 1016, 1284
StuI AGGCCT 1 cut(s) 1032
StyD4I CCNGG 3 cut(s) 1584, 1620, 1802
StyI CCWWGG 2 cut(s) 100, 1483
TaaI ACNGT 5 cut(s) 535, 597, 646, 1435, 1955
TaiI ACGT 1 cut(s) 656
TaqI TCGA 5 cut(s) 188, 548, 650, 1794, 2029
TatI WGTACW 2 cut(s) 359, 2055
TauI GCSGC 1 cut(s) 616
TfiI GAWTC 6 cut(s) 587, 734, 1003, 1279, 1343, 2023
Tru1I TTAA 7 cut(s) 621, 764, 950, 1209, 1257, 1629, 2010
Tru9I TTAA 7 cut(s) 621, 764, 950, 1209, 1257, 1629, 2010
TscAI CASTG 2 cut(s) 1960, 2131
TseFI GTSAC 2 cut(s) 943, 1740
TseI GCWGC 4 cut(s) 1103, 1469, 1609, 1667
Tsp45I GTSAC 2 cut(s) 943, 1740
TspRI CASTG 2 cut(s) 1960, 2131
Tth111I GACNNNGTC 1 cut(s) 533
Van91I CCANNNNNTGG 1 cut(s) 1452
VpaK11BI GGWCC 1 cut(s) 171
VspI ATTAAT 1 cut(s) 1257
XapI RAATTY 6 cut(s) 451, 544, 634, 702, 983, 1150
XceI RCATGY 4 cut(s) 501, 620, 864, 2066
XcmI CCANNNNNNNNNTGG 1 cut(s) 2126
XmnI GAANNNNTTC 2 cut(s) 915, 1707
XspI CTAG 7 cut(s) 24, 42, 195, 435, 713, 1016, 1284
ZrmI AGTACT 1 cut(s) 361
Zsp2I ATGCAT 2 cut(s) 1063, 1321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.