Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0008206

Physical Location & Seq

Genomic Coordinates Forward (+)

31026649 .. 31030685

Gene Structure 4037 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1210900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1284 bp

ATGGCGGCTTCTTCTCTGCTTAGATCTACAGTCTCAGCTCCGTTGATCGAAGCCTCCCGAGCCGACCTCGCCGCCTCCGCCTCCGCCTCTTTCAAGGTGTCAAGCGTCGGGTTCAGTCGCAATCTGAACTGTCCCAAGGTGCAATCCTCCATATTTGGCACTTCCATCCCAAGCGGGTTATCATCTTTACAGATTTGCAGCGCCAGGAGCGTCCAACCCATCAAGGCCACAGCCACTGAACTGCCACCCACGGTTCAGAAATCAGGGAGTGGTGGGAAGACAAAGATCGGAATCAATGGTTTTGGTAGAATCGGAAGGTTGGTTCTAAGGATTGCAACCACTAGAGATGATATCGAAGTGGTGGCAGTCAATGATCCTTTAGTTGATCCTAAGTACATGGCTTACATGTTTAAGTATGACTCTACACATGGAATATTCGATGGATCAATTAGTGTTGTGGATAATTCCACCTTGGAAATAAATGGGAAGGAAATCAAAGTTGTGAGCAAAAGAGATCCTGCTGAGATTCCTTGGGGTGATTATGGGGTTGAGTATGTTGTCGAATCTTCTGGGATTTTTACAACACTTGAGAAGGCGGCATTACATAAAAAGGGTGGTGCAAAGAAAGTGGTAATATCCGCCCCATCAGCTGATGCACCCATGTTTGTGGTAGGAGTAAATGAGAATACATACAAGCCAAATATGGACATTGTTTCAAATGCAAGCTGTACCACCAACTGCCTTGCTCCTCTTGCCAAGGTGATTCATGAGGAATTTGGTATTCTTGAAGGTTTGATGACCACTGTGCATGCAACTACAGCAACACAGAAGACAGTGGATGGCCCTTCAATGAAGGATTGGAGAGGAGGCCGAGGAGCTGGACAGAACATCATACCTAGTTCTACCGGTGCTGCAAAGGCAGTTGGAAAAGTTCTACCAGAACTCAATGGAAAGCTCACTGGTATGGCCTTCCGTGTCCCAACTCCTAATGTATCTGTCGTGGACTTAACTTGTCGACTTGAGAAGAGTTCTTCATATGAGGATGTCAAAGCCACGATCAGGTATGCTGCTGATGGGCCACTTAGAGGCATCCTCGGATACACAGAAGAAGATGTCGTCTCCAATGACTTTGTCGGTGACTCAAGGTCTAGTATATTCGATGCCAAGGCTGGATTGGCACTCAGTTCCTCCTTTGTTAAGCTTGTTTCGTGGTACGACAACGAGTGGGGATACAGCAACCGAGTTTTGGACCTCATAGAGCACATGGCGTTAGTGGCAGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004365 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0007275 GO:0008150 GO:0008152 GO:0008270 GO:0009536 GO:0009555 GO:0009719 GO:0009725 GO:0009735 GO:0009791 GO:0009908 GO:0010033 GO:0016020 GO:0016491 GO:0016620 GO:0016903 GO:0022414 GO:0022622 GO:0032501 GO:0032502 GO:0042221 GO:0042592 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043891 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0048229 GO:0048364 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048653 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048878 GO:0050801 GO:0050896 GO:0055081 GO:0055114 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0080022 GO:0080144 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00010 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04066 ko05010 map00010 map00710 map01100 map01110 map01120 map01130 map01200 map01230 map04066 map05010

Pfam Domains

Protein Families

Gp_dh_C Gp_dh_N

Protein Analysis

428

Amino Acids

45.24

Weight (kDa)

7.6

Isoelectric Point (pI)

35.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gp_dh_N	PF00044	95 - 195	4.8e-39	Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain
Gp_dh_C	PF02800	248 - 405	3.3e-68	Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008206)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16300 AT1G79530
fragaria_vesca	FvH4_3g14640
malus_domestica	MD05G1234400.v1.1 MD10G1210900.v1.1
prunus_persica	Prupe.4G131700_v2.0.a1 Prupe.4G131700_v2.0.a1
pyrus_communis	pycom05g21170 pycom10g17920 pycom10g17930
rosa_chinensis	RchiOBHm_Chr5g0024101
rosa_laevigata	RLG00000032770
rosa_multiflora	Rmu_sc0020340.1_g000003 Rmu_sc0026565.1_g000003
rosa_roxburghii	Rroxscaffold_1G00055140 Rroxscaffold_1G00055670
rosa_rugosa	Rorug05G0081300
rosa_samantha	Rh5AG172200 Rh5BG168700 Rh5CG184500 Rh5DG171000
rosa_wichuraiana	Rw0G003230 Rw5G015540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1015
AciI	CCGC	7 cut(s)	5, 72, 78, 84, 174, 596, 639
AclWI	GGATC	4 cut(s)	368, 380, 451, 509
AcsI	RAATTY	1 cut(s)	773
AfaI	GTAC	3 cut(s)	395, 730, 1214
AfiI	CCNNNNNNNGG	3 cut(s)	1059, 1085, 1246
AflIII	ACRYGT	1 cut(s)	405
AgeI	ACCGGT	1 cut(s)	905
AgsI	TTSAA	4 cut(s)	94, 717, 788, 849
AhdI	GACNNNNNGTC	1 cut(s)	1144
AjnI	CCWGG	1 cut(s)	203
AloI	GAACNNNNNNTCC	2 cut(s)	306, 338
AluBI	AGCT	6 cut(s)	38, 650, 726, 878, 955, 1201
AluI	AGCT	6 cut(s)	38, 650, 726, 878, 955, 1201
Alw21I	GWGCWC	1 cut(s)	1263
Alw26I	GTCTC	2 cut(s)	37, 1123
AlwI	GGATC	4 cut(s)	368, 380, 451, 509
AlwNI	CAGNNNCTG	1 cut(s)	236
Ama87I	CYCGRG	1 cut(s)	57
AoxI	GGCC	5 cut(s)	225, 841, 868, 966, 1076
ApeKI	GCWGC	4 cut(s)	198, 911, 1067, 1277
ApoI	RAATTY	1 cut(s)	773
AsiGI	ACCGGT	1 cut(s)	905
AspLEI	GCGC	1 cut(s)	203
AspS9I	GGNCC	3 cut(s)	842, 1076, 1249
AsuHPI	GGTGA	3 cut(s)	548, 772, 1148
AvaI	CYCGRG	1 cut(s)	57
AvaII	GGWCC	1 cut(s)	1249
BbsI	GAAGAC	2 cut(s)	284, 836
Bbv12I	GWGCWC	1 cut(s)	1263
BbvI	GCAGC	3 cut(s)	210, 898, 1054
BccI	CCATC	6 cut(s)	173, 227, 434, 652, 833, 1067
BciT130I	CCWGG	1 cut(s)	205
BciVI	GTATCC	2 cut(s)	1091, 1223
BcoDI	GTCTC	2 cut(s)	37, 1123
BfaI	CTAG	3 cut(s)	342, 897, 1149
BfmI	CTRYAG	2 cut(s)	27, 816
BfoI	RGCGCY	1 cut(s)	204
BfuI	GTATCC	2 cut(s)	1091, 1223
BglII	AGATCT	1 cut(s)	23
BisI	GCNGC	7 cut(s)	6, 72, 199, 597, 912, 1068, 1278
BlsI	GCNGC	7 cut(s)	7, 73, 200, 598, 913, 1069, 1279
Bme1390I	CCNGG	1 cut(s)	205
Bme18I	GGWCC	1 cut(s)	1249
BmeRI	GACNNNNNGTC	1 cut(s)	1144
BmeT110I	CYCGRG	1 cut(s)	57
BmgT120I	GGNCC	3 cut(s)	842, 1076, 1249
BmrFI	CCNGG	1 cut(s)	205
BmsI	GCATC	3 cut(s)	643, 1098, 1150
BpiI	GAAGAC	2 cut(s)	284, 836
BplI	GAGNNNNNCTC	4 cut(s)	51, 83, 1077, 1109
BpuEI	CTTGAG	3 cut(s)	608, 1040, 1126
BsaBI	GATNNNNATC	1 cut(s)	290
BsaJI	CCNNGG	8 cut(s)	135, 249, 471, 530, 756, 871, 1093, 1164
BsaWI	WCCGGW	1 cut(s)	905
Bsc4I	CCNNNNNNNGG	3 cut(s)	1059, 1085, 1246
Bse118I	RCCGGY	1 cut(s)	905
Bse1I	ACTGG	1 cut(s)	964
Bse8I	GATNNNNATC	1 cut(s)	290
BseBI	CCWGG	1 cut(s)	205
BseDI	CCNNGG	8 cut(s)	135, 249, 471, 530, 756, 871, 1093, 1164
BseGI	GGATG	4 cut(s)	165, 844, 1048, 1089
BseJI	GATNNNNATC	1 cut(s)	290
BseLI	CCNNNNNNNGG	3 cut(s)	1059, 1085, 1246
BseMII	CTCAG	3 cut(s)	48, 513, 1195
BseNI	ACTGG	1 cut(s)	964
BseRI	GAGGAG	3 cut(s)	738, 879, 888
BseXI	GCAGC	3 cut(s)	210, 898, 1054
BshFI	GGCC	5 cut(s)	227, 843, 870, 968, 1078
BshTI	ACCGGT	1 cut(s)	905
BsiHKAI	GWGCWC	1 cut(s)	1263
BsiHKCI	CYCGRG	1 cut(s)	57
BsiSI	CCGG	1 cut(s)	906
BslFI	GGGAC	2 cut(s)	117, 962
BslI	CCNNNNNNNGG	3 cut(s)	1059, 1085, 1246
BsmAI	GTCTC	2 cut(s)	37, 1123
BsmBI	CGTCTC	1 cut(s)	1123
BsmFI	GGGAC	2 cut(s)	117, 962
BsnI	GGCC	5 cut(s)	227, 843, 870, 968, 1078
BsoBI	CYCGRG	1 cut(s)	57
Bsp1286I	GDGCHC	1 cut(s)	1263
Bsp143I	GATC	8 cut(s)	23, 45, 285, 373, 385, 443, 514, 1056
BspACI	CCGC	7 cut(s)	5, 72, 78, 84, 174, 596, 639
BspANI	GGCC	5 cut(s)	227, 843, 870, 968, 1078
BspCNI	CTCAG	3 cut(s)	47, 514, 1194
BspHI	TCATGA	1 cut(s)	766
BspPI	GGATC	4 cut(s)	368, 380, 451, 509
BsrFI	RCCGGY	1 cut(s)	905
BsrI	ACTGG	1 cut(s)	964
BssAI	RCCGGY	1 cut(s)	905
BssECI	CCNNGG	8 cut(s)	135, 249, 471, 530, 756, 871, 1093, 1164
BssMI	GATC	8 cut(s)	23, 45, 285, 373, 385, 443, 514, 1056
BssT1I	CCWWGG	5 cut(s)	135, 471, 530, 756, 1164
Bst2UI	CCWGG	1 cut(s)	205
Bst4CI	ACNGT	5 cut(s)	31, 131, 253, 805, 835
Bst6I	CTCTTC	1 cut(s)	1019
BstC8I	GCNNGC	2 cut(s)	724, 810
BstDEI	CTNAG	7 cut(s)	20, 34, 326, 390, 522, 1082, 1181
BstDSI	CCRYGG	1 cut(s)	249
BstF5I	GGATG	4 cut(s)	165, 844, 1048, 1089
BstH2I	RGCGCY	1 cut(s)	204
BstHHI	GCGC	1 cut(s)	203
BstKTI	GATC	8 cut(s)	26, 48, 288, 376, 388, 446, 517, 1059
BstMAI	GTCTC	2 cut(s)	37, 1123
BstMBI	GATC	8 cut(s)	23, 45, 285, 373, 385, 443, 514, 1056
BstNI	CCWGG	1 cut(s)	205
BstNSI	RCATGY	2 cut(s)	409, 812
BstSCI	CCNGG	1 cut(s)	203
BstSFI	CTRYAG	2 cut(s)	27, 816
BstV1I	GCAGC	3 cut(s)	210, 898, 1054
BstV2I	GAAGAC	2 cut(s)	284, 836
BstX2I	RGATCY	2 cut(s)	23, 514
BstXI	CCANNNNNNTGG	1 cut(s)	667
BstYI	RGATCY	2 cut(s)	23, 514
BsuI	GTATCC	2 cut(s)	1091, 1223
BsuRI	GGCC	5 cut(s)	227, 843, 870, 968, 1078
BtgI	CCRYGG	1 cut(s)	249
BtsCI	GGATG	4 cut(s)	165, 844, 1048, 1089
BtsIMutI	CAGTG	4 cut(s)	234, 801, 840, 957
Cac8I	GCNNGC	2 cut(s)	724, 810
CaiI	CAGNNNCTG	1 cut(s)	236
CciI	TCATGA	1 cut(s)	766
CfoI	GCGC	1 cut(s)	203
Cfr10I	RCCGGY	1 cut(s)	905
Cfr13I	GGNCC	3 cut(s)	842, 1076, 1249
CseI	GACGC	2 cut(s)	94, 199
Csp6I	GTAC	3 cut(s)	394, 729, 1213
CspAI	ACCGGT	1 cut(s)	905
CspCI	CAANNNNNGTGG	2 cut(s)	609, 644
CviAII	CATG	7 cut(s)	397, 406, 428, 661, 767, 809, 1264
CviQI	GTAC	3 cut(s)	394, 729, 1213
DdeI	CTNAG	7 cut(s)	20, 34, 326, 390, 522, 1082, 1181
DpnI	GATC	8 cut(s)	25, 47, 287, 375, 387, 445, 516, 1058
DpnII	GATC	8 cut(s)	23, 45, 285, 373, 385, 443, 514, 1056
DriI	GACNNNNNGTC	1 cut(s)	1144
Eam1104I	CTCTTC	1 cut(s)	1019
Eam1105I	GACNNNNNGTC	1 cut(s)	1144
EarI	CTCTTC	1 cut(s)	1019
EciI	GGCGGA	3 cut(s)	67, 73, 628
Eco130I	CCWWGG	5 cut(s)	135, 471, 530, 756, 1164
Eco32I	GATATC	1 cut(s)	352
Eco47I	GGWCC	1 cut(s)	1249
Eco88I	CYCGRG	1 cut(s)	57
EcoRII	CCWGG	1 cut(s)	203
EcoRV	GATATC	1 cut(s)	352
EcoT14I	CCWWGG	5 cut(s)	135, 471, 530, 756, 1164
ErhI	CCWWGG	5 cut(s)	135, 471, 530, 756, 1164
Esp3I	CGTCTC	1 cut(s)	1123
FaeI	CATG	7 cut(s)	400, 409, 431, 664, 770, 812, 1267
FaqI	GGGAC	2 cut(s)	117, 962
FatI	CATG	7 cut(s)	396, 405, 427, 660, 766, 808, 1263
FauI	CCCGC	1 cut(s)	167
FauNDI	CATATG	1 cut(s)	1036
FblI	GTMKAC	1 cut(s)	1015
Fnu4HI	GCNGC	7 cut(s)	6, 72, 199, 597, 912, 1068, 1278
FokI	GGATG	4 cut(s)	152, 851, 1055, 1076
Fsp4HI	GCNGC	7 cut(s)	6, 72, 199, 597, 912, 1068, 1278
FspBI	CTAG	3 cut(s)	342, 897, 1149
GlaI	GCGC	1 cut(s)	202
GluI	GCNGC	7 cut(s)	6, 72, 199, 597, 912, 1068, 1278
HaeII	RGCGCY	1 cut(s)	204
HaeIII	GGCC	5 cut(s)	227, 843, 870, 968, 1078
HapII	CCGG	1 cut(s)	906
HgaI	GACGC	2 cut(s)	94, 199
HhaI	GCGC	1 cut(s)	203
Hin1II	CATG	7 cut(s)	400, 409, 431, 664, 770, 812, 1267
Hin6I	GCGC	1 cut(s)	201
HinP1I	GCGC	1 cut(s)	201
HincII	GTYRAC	1 cut(s)	1016
HindII	GTYRAC	1 cut(s)	1016
HindIII	AAGCTT	1 cut(s)	1199
HinfI	GANTC	7 cut(s)	291, 309, 419, 526, 563, 763, 1139
HpaII	CCGG	1 cut(s)	906
HphI	GGTGA	3 cut(s)	548, 772, 1148
Hpy166II	GTNNAC	2 cut(s)	1003, 1016
Hpy188I	TCNGA	5 cut(s)	126, 258, 290, 314, 1097
Hpy188III	TCNNGA	3 cut(s)	57, 767, 785
Hpy8I	GTNNAC	2 cut(s)	1003, 1016
Hpy99I	CGWCG	1 cut(s)	110
HpyAV	CCTTC	7 cut(s)	309, 481, 586, 782, 847, 855, 979
HpyCH4III	ACNGT	5 cut(s)	31, 131, 253, 805, 835
HpyCH4V	TGCA	9 cut(s)	142, 198, 335, 620, 656, 722, 808, 812, 914
HpyF3I	CTNAG	7 cut(s)	20, 34, 326, 390, 522, 1082, 1181
Hsp92II	CATG	7 cut(s)	400, 409, 431, 664, 770, 812, 1267
HspAI	GCGC	1 cut(s)	201
Kzo9I	GATC	8 cut(s)	23, 45, 285, 373, 385, 443, 514, 1056
LmnI	GCTCC	4 cut(s)	43, 207, 751, 875
Lsp1109I	GCAGC	3 cut(s)	210, 898, 1054
LweI	GCATC	3 cut(s)	643, 1098, 1150
MaeI	CTAG	3 cut(s)	342, 897, 1149
MaeIII	GTNAC	1 cut(s)	1136
MalI	GATC	8 cut(s)	25, 47, 287, 375, 387, 445, 516, 1058
MboI	GATC	8 cut(s)	23, 45, 285, 373, 385, 443, 514, 1056
MboII	GAAGA	8 cut(s)	3, 289, 558, 841, 1023, 1036, 1118, 1121
MflI	RGATCY	2 cut(s)	23, 514
MhlI	GDGCHC	1 cut(s)	1263
MluCI	AATT	3 cut(s)	447, 463, 773
MlyI	GAGTC	2 cut(s)	413, 1133
MmeI	TCCRAC	2 cut(s)	238, 904
MseI	TTAA	3 cut(s)	411, 1007, 1197
MslI	CAYNNNNRTG	2 cut(s)	665, 962
MspA1I	CMGCKG	1 cut(s)	650
MspI	CCGG	1 cut(s)	906
MspR9I	CCNGG	1 cut(s)	205
MvaI	CCWGG	1 cut(s)	205
NdeI	CATATG	1 cut(s)	1036
NdeII	GATC	8 cut(s)	23, 45, 285, 373, 385, 443, 514, 1056
NlaIII	CATG	7 cut(s)	400, 409, 431, 664, 770, 812, 1267
NmeAIII	GCCGAG	1 cut(s)	896
NmuCI	GTSAC	1 cut(s)	1136
NspI	RCATGY	2 cut(s)	409, 812
PaeI	GCATGC	1 cut(s)	812
PagI	TCATGA	1 cut(s)	766
PciI	ACATGT	1 cut(s)	405
PfeI	GAWTC	5 cut(s)	291, 309, 526, 563, 763
PflFI	GACNNNGTC	1 cut(s)	1130
PinAI	ACCGGT	1 cut(s)	905
PkrI	GCNGC	7 cut(s)	7, 73, 200, 598, 913, 1069, 1279
PleI	GAGTC	2 cut(s)	413, 1133
PpsI	GAGTC	2 cut(s)	413, 1133
PscI	ACATGT	1 cut(s)	405
Psp6I	CCWGG	1 cut(s)	203
PspGI	CCWGG	1 cut(s)	203
PspPI	GGNCC	3 cut(s)	842, 1076, 1249
PstNI	CAGNNNCTG	1 cut(s)	236
PsuI	RGATCY	2 cut(s)	23, 514
PsyI	GACNNNGTC	1 cut(s)	1130
PvuII	CAGCTG	1 cut(s)	650
RsaI	GTAC	3 cut(s)	395, 730, 1214
RsaNI	GTAC	3 cut(s)	394, 729, 1213
RseI	CAYNNNNRTG	2 cut(s)	665, 962
SalI	GTCGAC	1 cut(s)	1014
SaqAI	TTAA	3 cut(s)	411, 1007, 1197
SatI	GCNGC	7 cut(s)	6, 72, 199, 597, 912, 1068, 1278
Sau3AI	GATC	8 cut(s)	23, 45, 285, 373, 385, 443, 514, 1056
Sau96I	GGNCC	3 cut(s)	842, 1076, 1249
SchI	GAGTC	2 cut(s)	413, 1133
ScrFI	CCNGG	1 cut(s)	205
SduI	GDGCHC	1 cut(s)	1263
SfaNI	GCATC	3 cut(s)	643, 1098, 1150
SfcI	CTRYAG	2 cut(s)	27, 816
SinI	GGWCC	1 cut(s)	1249
SmiMI	CAYNNNNRTG	2 cut(s)	665, 962
SmlI	CTYRAG	3 cut(s)	587, 1019, 1141
SmoI	CTYRAG	3 cut(s)	587, 1019, 1141
SphI	GCATGC	1 cut(s)	812
Sse9I	AATT	3 cut(s)	447, 463, 773
SsiI	CCGC	7 cut(s)	5, 72, 78, 84, 174, 596, 639
SspI	AATATT	1 cut(s)	435
SspMI	CTAG	3 cut(s)	342, 897, 1149
StyD4I	CCNGG	1 cut(s)	203
StyI	CCWWGG	5 cut(s)	135, 471, 530, 756, 1164
TaaI	ACNGT	5 cut(s)	31, 131, 253, 805, 835
TaqI	TCGA	6 cut(s)	48, 354, 438, 561, 1015, 1158
TasI	AATT	3 cut(s)	447, 463, 773
TatI	WGTACW	1 cut(s)	393
TauI	GCSGC	3 cut(s)	8, 74, 599
TfiI	GAWTC	5 cut(s)	291, 309, 526, 563, 763
Tru1I	TTAA	3 cut(s)	411, 1007, 1197
Tru9I	TTAA	3 cut(s)	411, 1007, 1197
TscAI	CASTG	4 cut(s)	241, 808, 840, 964
TseFI	GTSAC	1 cut(s)	1136
TseI	GCWGC	4 cut(s)	198, 911, 1067, 1277
Tsp45I	GTSAC	1 cut(s)	1136
TspDTI	ATGAA	3 cut(s)	755, 866, 1023
TspGWI	ACGGA	2 cut(s)	30, 962
TspRI	CASTG	4 cut(s)	241, 808, 840, 964
Tth111I	GACNNNGTC	1 cut(s)	1130
VpaK11BI	GGWCC	1 cut(s)	1249
XapI	RAATTY	1 cut(s)	773
XceI	RCATGY	2 cut(s)	409, 812
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1171
XmiI	GTMKAC	1 cut(s)	1015
XspI	CTAG	3 cut(s)	342, 897, 1149

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.