MD10G1217500.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013377
Physical Location & Seq
Reverse (-)
31583926 .. 31588221
4296 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1217500.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGCCAACAGTGGCCTATATAGTCCATTGTCGCGCAAGCCAGTTTAGTTCAAGTCCAGAGTTTTGCTCGCTCGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGTTTAACTTCATCTCTCTCACACTCTCAGAGAGTACTGCAATGGCACCCATGAGTCTTCCTCCTGGATTCAGATTCCACCCAACAGATGAAGAGCTTGTTGCTTACTATCTAGATCGAAAAATCAATGGTCGCACCATTGAGCTAGAAATTATCCCAGAGGTCGACCTCTACAAATGTGAGCCATGGGATTTACCTGATAAGTCGTTTCTTCCGAGCAAAGACATGGAGTGGTACTTCTACAGCCCGAGGGATAGGAAGTACCCCAACGGGTCGAGAACGAATAGGGCTACTCGAGCTGGGTACTGGAAAGCGACTGGGAAAGACCGGGCGGTGAACAGTCAGAGGCGTGCTGTCGGCATGAAGAAGACATTGGTTTACTATAGAGGTAGAGCCCCCCATGGTATTAGAACCAACTGGGTTATGCATGAGTACCGGCTGGTCGATTCTGTGTGTGGCAATGGGTCATCATCTATAAAGGATTCTTACGCATTGTGCCGAGTGTTCAAGAAAACAATACAAATGCCCAAGAGTAACAAAGAATACACGCCGATTGGGATTAACAATGCAGACAATGATTCAATATGGGTCTCCAATGAACAATTGTTAGGGGAAGACACTAGTGGCATTAATGAGGGAGCTTCAAGAGGGATTGAAACTGATCAAGATGAGAATGATTCCAACCATGATTATCCCAAATTTCTATCTGACACCTCTTCTTCAGATCTCACTCAAGGCACACCTACTGAAACTGGCATAGCTGATGATTTACAAGCTCCATTTGCTTCTGATGAAGCAAACAGTTCAGCTGATCTATACTCTTTTGGTGTCCACTGCTCCTCAGATCTACTTCAGGAAACATATATACCGCCCAATGCGAGCTCACTGAATACCTATCAATTTCCTTACCCACCCTTAGAACTTGAAGACTTCCCGCAGATCAATTTAGCTGCAGAGACAAACACAGCAAAGCCAGAAATCATCGACAAGTACATGTCGTACGACAAGTTCAAGGATTACATGAATGGATCATTTGAAGAGATCTTCTCTTTATGTTCATCTCAAGACAACTCTGTGGCCCTCTCCATGCAAGACTAA

Protein Analysis

403

Amino Acids

45.16

Weight (kDa)

4.81

Isoelectric Point (pI)

38.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 58 - 184 2.2e-50 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013377)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54330
fragaria_vesca FvH4_3g13980
malus_domestica MD05G1239300.v1.1 MD10G1217500.v1.1
prunus_persica Prupe.4G126800_v2.0.a1
pyrus_communis pycom05g21560 pycom10g18340
rosa_chinensis RchiOBHm_Chr5g0023211
rosa_laevigata RLG00000032709
rosa_multiflora Rmu_sc0001735.1_g000001 Rmu_sc0007300.1_g000012
rosa_roxburghii Rroxscaffold_1G00056140
rosa_rugosa Rorug05G0078000
rosa_samantha Rh5AG164100 Rh5BG163600 Rh5CG178800 Rh5DG165400
rosa_wichuraiana Rw5G014810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 157
AccI GTMKAC 1 cut(s) 276
AccII CGCG 1 cut(s) 33
AciI CCGC 3 cut(s) 443, 980, 1046
AclWI GGATC 1 cut(s) 1147
AcsI RAATTY 1 cut(s) 809
AcuI CTGAAG 2 cut(s) 816, 947
AfaI GTAC 7 cut(s) 148, 347, 374, 416, 545, 1103, 1112
AflIII ACRYGT 1 cut(s) 1104
AgsI TTSAA 8 cut(s) 51, 619, 693, 756, 767, 1037, 1123, 1148
AhlI ACTAGT 1 cut(s) 731
AjnI CCWGG 1 cut(s) 175
AjuI GAANNNNNNNTTGG 2 cut(s) 467, 499
AluBI AGCT 9 cut(s) 208, 256, 410, 752, 872, 887, 920, 993, 1061
AluI AGCT 9 cut(s) 208, 256, 410, 752, 872, 887, 920, 993, 1061
Alw21I GWGCWC 1 cut(s) 995
Alw26I GTCTC 2 cut(s) 706, 1061
AlwI GGATC 1 cut(s) 1147
Ama87I CYCGRG 2 cut(s) 358, 405
AoxI GGCC 2 cut(s) 12, 1188
ApeKI GCWGC 1 cut(s) 1061
ApoI RAATTY 1 cut(s) 809
AseI ATTAAT 1 cut(s) 741
AspLEI GCGC 1 cut(s) 35
AspS9I GGNCC 1 cut(s) 1189
AsuC2I CCSGG 1 cut(s) 440
AsuHPI GGTGA 1 cut(s) 457
AvaI CYCGRG 2 cut(s) 358, 405
BanI GGYRCC 1 cut(s) 157
BanII GRGCYC 2 cut(s) 508, 995
BbsI GAAGAC 4 cut(s) 161, 485, 732, 1044
Bbv12I GWGCWC 1 cut(s) 995
BbvI GCAGC 1 cut(s) 1048
BciT130I CCWGG 1 cut(s) 177
BclI TGATCA 1 cut(s) 772
BcnI CCSGG 1 cut(s) 440
BcoDI GTCTC 2 cut(s) 706, 1061
BcuI ACTAGT 1 cut(s) 731
BfaI CTAG 3 cut(s) 224, 257, 732
BfmI CTRYAG 3 cut(s) 352, 493, 1062
BglII AGATCT 3 cut(s) 835, 955, 1152
BisI GCNGC 1 cut(s) 1062
BlsI GCNGC 1 cut(s) 1063
BmcAI AGTACT 1 cut(s) 148
Bme1390I CCNGG 2 cut(s) 177, 440
BmeT110I CYCGRG 2 cut(s) 358, 405
BmgT120I GGNCC 1 cut(s) 1189
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 2 cut(s) 177, 440
BmrI ACTGGG 2 cut(s) 438, 538
BmuI ACTGGG 2 cut(s) 438, 538
BpiI GAAGAC 4 cut(s) 161, 485, 732, 1044
BplI GAGNNNNNCTC 6 cut(s) 50, 82, 157, 189, 1142, 1174
BpuEI CTTGAG 2 cut(s) 828, 1158
BpuMI CCSGG 1 cut(s) 440
BsaI GGTCTC 1 cut(s) 706
BsaJI CCNNGG 3 cut(s) 296, 359, 511
Bse118I RCCGGY 1 cut(s) 546
Bse1I ACTGG 5 cut(s) 40, 422, 433, 533, 868
Bse3DI GCAATG 2 cut(s) 159, 577
BseBI CCWGG 1 cut(s) 177
BseDI CCNNGG 3 cut(s) 296, 359, 511
BseMI GCAATG 2 cut(s) 159, 577
BseMII CTCAG 2 cut(s) 153, 966
BseNI ACTGG 5 cut(s) 40, 422, 433, 533, 868
BseRI GAGGAG 1 cut(s) 940
BseXI GCAGC 1 cut(s) 1048
BseYI CCCAGC 1 cut(s) 410
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 2 cut(s) 14, 1190
BshNI GGYRCC 1 cut(s) 157
BsiHKAI GWGCWC 1 cut(s) 995
BsiHKCI CYCGRG 2 cut(s) 358, 405
BsiSI CCGG 2 cut(s) 439, 547
BsiWI CGTACG 1 cut(s) 1110
BsmAI GTCTC 2 cut(s) 706, 1061
BsnI GGCC 2 cut(s) 14, 1190
Bso31I GGTCTC 1 cut(s) 706
BsoBI CYCGRG 2 cut(s) 358, 405
Bsp1286I GDGCHC 2 cut(s) 508, 995
Bsp143I GATC 8 cut(s) 226, 772, 835, 922, 955, 1050, 1139, 1152
Bsp19I CCATGG 2 cut(s) 296, 511
BspACI CCGC 3 cut(s) 443, 980, 1046
BspANI GGCC 2 cut(s) 14, 1190
BspCNI CTCAG 2 cut(s) 152, 965
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 1 cut(s) 159
BspMAI CTGCAG 1 cut(s) 1066
BspPI GGATC 1 cut(s) 1147
BspQI GCTCTTC 1 cut(s) 198
BspT107I GGYRCC 1 cut(s) 157
BspTNI GGTCTC 1 cut(s) 706
BsrDI GCAATG 2 cut(s) 159, 577
BsrFI RCCGGY 1 cut(s) 546
BsrI ACTGG 5 cut(s) 40, 422, 433, 533, 868
BssAI RCCGGY 1 cut(s) 546
BssECI CCNNGG 3 cut(s) 296, 359, 511
BssMI GATC 8 cut(s) 226, 772, 835, 922, 955, 1050, 1139, 1152
BssT1I CCWWGG 2 cut(s) 296, 511
Bst2UI CCWGG 1 cut(s) 177
Bst4CI ACNGT 3 cut(s) 10, 452, 914
Bst6I CTCTTC 3 cut(s) 198, 832, 1143
BstC8I GCNNGC 5 cut(s) 37, 68, 72, 462, 991
BstDEI CTNAG 3 cut(s) 139, 952, 1027
BstDSI CCRYGG 2 cut(s) 296, 511
BstFNI CGCG 1 cut(s) 33
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 8 cut(s) 229, 775, 838, 925, 958, 1053, 1142, 1155
BstMAI GTCTC 2 cut(s) 706, 1061
BstMBI GATC 8 cut(s) 226, 772, 835, 922, 955, 1050, 1139, 1152
BstMWI GCNNNNNNNGC 2 cut(s) 407, 893
BstNI CCWGG 1 cut(s) 177
BstNSI RCATGY 1 cut(s) 1108
BstSCI CCNGG 2 cut(s) 175, 438
BstSFI CTRYAG 3 cut(s) 352, 493, 1062
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 1 cut(s) 1048
BstV2I GAAGAC 4 cut(s) 161, 485, 732, 1044
BstX2I RGATCY 3 cut(s) 835, 955, 1152
BstYI RGATCY 3 cut(s) 835, 955, 1152
BsuRI GGCC 2 cut(s) 14, 1190
BtgI CCRYGG 2 cut(s) 296, 511
BtsI GCAGTG 1 cut(s) 943
BtsIMutI CAGTG 3 cut(s) 15, 943, 995
Cac8I GCNNGC 5 cut(s) 37, 68, 72, 462, 991
CfoI GCGC 1 cut(s) 35
Cfr10I RCCGGY 1 cut(s) 546
Cfr13I GGNCC 1 cut(s) 1189
Csp6I GTAC 7 cut(s) 147, 346, 373, 415, 544, 1102, 1111
CviQI GTAC 7 cut(s) 147, 346, 373, 415, 544, 1102, 1111
DdeI CTNAG 3 cut(s) 139, 952, 1027
DpnI GATC 8 cut(s) 228, 774, 837, 924, 957, 1052, 1141, 1154
DpnII GATC 8 cut(s) 226, 772, 835, 922, 955, 1050, 1139, 1152
Eam1104I CTCTTC 3 cut(s) 198, 832, 1143
EarI CTCTTC 3 cut(s) 198, 832, 1143
Ecl136II GAGCTC 1 cut(s) 993
Eco130I CCWWGG 2 cut(s) 296, 511
Eco24I GRGCYC 2 cut(s) 508, 995
Eco31I GGTCTC 1 cut(s) 706
Eco53kI GAGCTC 1 cut(s) 993
Eco57I CTGAAG 2 cut(s) 816, 947
Eco88I CYCGRG 2 cut(s) 358, 405
EcoICRI GAGCTC 1 cut(s) 993
EcoRII CCWGG 1 cut(s) 175
EcoT14I CCWWGG 2 cut(s) 296, 511
EcoT22I ATGCAT 1 cut(s) 540
EcoT38I GRGCYC 2 cut(s) 508, 995
ErhI CCWWGG 2 cut(s) 296, 511
FauI CCCGC 1 cut(s) 1053
FbaI TGATCA 1 cut(s) 772
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 1 cut(s) 1062
FriOI GRGCYC 2 cut(s) 508, 995
Fsp4HI GCNGC 1 cut(s) 1062
FspBI CTAG 3 cut(s) 224, 257, 732
GlaI GCGC 1 cut(s) 34
GluI GCNGC 1 cut(s) 1062
GsaI CCCAGC 1 cut(s) 414
HaeIII GGCC 2 cut(s) 14, 1190
HapII CCGG 2 cut(s) 439, 547
HhaI GCGC 1 cut(s) 35
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 7 cut(s) 166, 180, 186, 557, 593, 689, 788
HpaII CCGG 2 cut(s) 439, 547
HphI GGTGA 1 cut(s) 457
Hpy166II GTNNAC 4 cut(s) 277, 448, 490, 943
Hpy188I TCNGA 8 cut(s) 142, 185, 327, 456, 820, 835, 901, 955
Hpy188III TCNNGA 8 cut(s) 56, 224, 387, 619, 756, 776, 965, 1175
Hpy8I GTNNAC 4 cut(s) 277, 448, 490, 943
HpyCH4III ACNGT 3 cut(s) 10, 452, 914
HpyCH4V TGCA 5 cut(s) 152, 538, 680, 1064, 1201
HpyF10VI GCNNNNNNNGC 2 cut(s) 407, 893
HpyF3I CTNAG 3 cut(s) 139, 952, 1027
HspAI GCGC 1 cut(s) 33
Ksp22I TGATCA 1 cut(s) 772
Kzo9I GATC 8 cut(s) 226, 772, 835, 922, 955, 1050, 1139, 1152
LguI GCTCTTC 1 cut(s) 198
LmnI GCTCC 3 cut(s) 749, 892, 953
Lsp1109I GCAGC 1 cut(s) 1048
MaeI CTAG 3 cut(s) 224, 257, 732
MaeIII GTNAC 1 cut(s) 644
MalI GATC 8 cut(s) 228, 774, 837, 924, 957, 1052, 1141, 1154
MboI GATC 8 cut(s) 226, 772, 835, 922, 955, 1050, 1139, 1152
MfeI CAATTG 1 cut(s) 713
MflI RGATCY 3 cut(s) 835, 955, 1152
MhlI GDGCHC 2 cut(s) 508, 995
MluCI AATT 5 cut(s) 261, 713, 809, 1010, 1054
MlyI GAGTC 1 cut(s) 175
MmeI TCCRAC 1 cut(s) 816
Mph1103I ATGCAT 1 cut(s) 540
MseI TTAA 3 cut(s) 117, 672, 741
MspA1I CMGCKG 1 cut(s) 920
MspI CCGG 2 cut(s) 439, 547
MspR9I CCNGG 2 cut(s) 177, 440
MunI CAATTG 1 cut(s) 713
MvaI CCWGG 1 cut(s) 177
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 2 cut(s) 407, 893
NciI CCSGG 1 cut(s) 440
NcoI CCATGG 2 cut(s) 296, 511
NdeII GATC 8 cut(s) 226, 772, 835, 922, 955, 1050, 1139, 1152
NlaIV GGNNCC 1 cut(s) 159
NmeAIII GCCGAG 1 cut(s) 635
NsiI ATGCAT 1 cut(s) 540
NspI RCATGY 1 cut(s) 1108
PaeR7I CTCGAG 1 cut(s) 405
PciI ACATGT 1 cut(s) 1104
PciSI GCTCTTC 1 cut(s) 198
PcsI WCGNNNNNNNCGW 1 cut(s) 323
PfeI GAWTC 6 cut(s) 180, 186, 557, 593, 689, 788
Pfl23II CGTACG 1 cut(s) 1110
PfoI TCCNGGA 1 cut(s) 175
PkrI GCNGC 1 cut(s) 1063
PleI GAGTC 1 cut(s) 174
PpsI GAGTC 1 cut(s) 174
PscI ACATGT 1 cut(s) 1104
PshBI ATTAAT 1 cut(s) 741
Psp124BI GAGCTC 1 cut(s) 995
Psp6I CCWGG 1 cut(s) 175
PspFI CCCAGC 1 cut(s) 410
PspGI CCWGG 1 cut(s) 175
PspLI CGTACG 1 cut(s) 1110
PspN4I GGNNCC 1 cut(s) 159
PspPI GGNCC 1 cut(s) 1189
PspXI VCTCGAGB 1 cut(s) 405
PsrI GAACNNNNNNTAC 2 cut(s) 1103, 1135
PstI CTGCAG 1 cut(s) 1066
PsuI RGATCY 3 cut(s) 835, 955, 1152
PvuII CAGCTG 1 cut(s) 920
RsaI GTAC 7 cut(s) 148, 347, 374, 416, 545, 1103, 1112
RsaNI GTAC 7 cut(s) 147, 346, 373, 415, 544, 1102, 1111
SacI GAGCTC 1 cut(s) 995
SalI GTCGAC 1 cut(s) 275
SapI GCTCTTC 1 cut(s) 198
SaqAI TTAA 3 cut(s) 117, 672, 741
SatI GCNGC 1 cut(s) 1062
Sau3AI GATC 8 cut(s) 226, 772, 835, 922, 955, 1050, 1139, 1152
Sau96I GGNCC 1 cut(s) 1189
ScaI AGTACT 1 cut(s) 148
SchI GAGTC 1 cut(s) 175
ScrFI CCNGG 2 cut(s) 177, 440
SduI GDGCHC 2 cut(s) 508, 995
SfcI CTRYAG 3 cut(s) 352, 493, 1062
Sfr274I CTCGAG 1 cut(s) 405
SlaI CTCGAG 1 cut(s) 405
SmlI CTYRAG 3 cut(s) 405, 843, 1173
SmoI CTYRAG 3 cut(s) 405, 843, 1173
SpeI ACTAGT 1 cut(s) 731
Sse9I AATT 5 cut(s) 261, 713, 809, 1010, 1054
SsiI CCGC 3 cut(s) 443, 980, 1046
SspMI CTAG 3 cut(s) 224, 257, 732
SstI GAGCTC 1 cut(s) 995
StyD4I CCNGG 2 cut(s) 175, 438
StyI CCWWGG 2 cut(s) 296, 511
TaaI ACNGT 3 cut(s) 10, 452, 914
TaqI TCGA 6 cut(s) 229, 276, 386, 406, 555, 1095
TasI AATT 5 cut(s) 261, 713, 809, 1010, 1054
TatI WGTACW 2 cut(s) 146, 1101
TfiI GAWTC 6 cut(s) 180, 186, 557, 593, 689, 788
Tru1I TTAA 3 cut(s) 117, 672, 741
Tru9I TTAA 3 cut(s) 117, 672, 741
TscAI CASTG 3 cut(s) 15, 950, 1002
TseI GCWGC 1 cut(s) 1061
TspDTI ATGAA 7 cut(s) 112, 216, 488, 723, 918, 1148, 1158
TspRI CASTG 3 cut(s) 15, 950, 1002
VspI ATTAAT 1 cut(s) 741
XapI RAATTY 1 cut(s) 809
XbaI TCTAGA 1 cut(s) 223
XceI RCATGY 1 cut(s) 1108
XhoI CTCGAG 1 cut(s) 405
XmiI GTMKAC 1 cut(s) 276
XspI CTAG 3 cut(s) 224, 257, 732
ZrmI AGTACT 1 cut(s) 148
Zsp2I ATGCAT 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.