MD10G1238500.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002942
Physical Location & Seq
Forward (+)
33463337 .. 33469170
5834 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1238500.v1.1.491

Sequence Viewer

Length: 357 bp
ATGGAAGAGGTTTTAGGGCAGAAGAGGGTTGAAGATGTGAGCTGGCTCTGCTCCCTCTCTGAGTCTGAACTTGACATGTTGATTAGCATAAAAAGGATTGTTCTTCAACGTGCAAGAATGATTGGGTGCGATGAACTTGCCCAAAAGTTTGATCTCAAGGTGATTCGAGCTCTTGCATTTGTTCTGATGGAATGTGTCAAAGATAAGGTAAATAATTTGTCACCGCCCGAGTCTGCTGCATTTATGGGAAGTTGCAATCTGTTAAAACATAACCTTGGTGATATTATGAGTCTTGAAGAGATAATGGCATGTATCGGTATTGATTCGAGAAAGGGTCCAATAAAAAGGTATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

119

Amino Acids

13.26

Weight (kDa)

5.85

Isoelectric Point (pI)

60.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 224
AflIII ACRYGT 1 cut(s) 75
AgsI TTSAA 3 cut(s) 32, 107, 296
AluBI AGCT 2 cut(s) 42, 170
AluI AGCT 2 cut(s) 42, 170
Alw21I GWGCWC 1 cut(s) 172
Ama87I CYCGRG 1 cut(s) 227
ApeKI GCWGC 1 cut(s) 236
AspS9I GGNCC 1 cut(s) 335
AsuHPI GGTGA 3 cut(s) 172, 213, 290
AvaI CYCGRG 1 cut(s) 227
AvaII GGWCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 172
Bbv12I GWGCWC 1 cut(s) 172
BbvI GCAGC 1 cut(s) 223
BccI CCATC 1 cut(s) 181
BcgI CGANNNNNNTGC 4 cut(s) 119, 153, 218, 252
BisI GCNGC 1 cut(s) 237
BlsI GCNGC 1 cut(s) 238
Bme18I GGWCC 1 cut(s) 335
BmeT110I CYCGRG 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 1 cut(s) 336
BpuEI CTTGAG 1 cut(s) 140
BsaJI CCNNGG 1 cut(s) 274
BseDI CCNNGG 1 cut(s) 274
BseMII CTCAG 1 cut(s) 51
BseXI GCAGC 1 cut(s) 223
BsiHKAI GWGCWC 1 cut(s) 172
BsiHKCI CYCGRG 1 cut(s) 227
BsoBI CYCGRG 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 172
Bsp143I GATC 1 cut(s) 151
BspACI CCGC 1 cut(s) 224
BspCNI CTCAG 1 cut(s) 52
BspLI GGNNCC 1 cut(s) 336
BssECI CCNNGG 1 cut(s) 274
BssMI GATC 1 cut(s) 151
BssT1I CCWWGG 1 cut(s) 274
Bst6I CTCTTC 2 cut(s) 17, 291
BstC8I GCNNGC 1 cut(s) 44
BstDEI CTNAG 1 cut(s) 60
BstKTI GATC 1 cut(s) 154
BstMBI GATC 1 cut(s) 151
BstMWI GCNNNNNNNGC 1 cut(s) 48
BstNSI RCATGY 2 cut(s) 79, 312
BstV1I GCAGC 1 cut(s) 223
BtgZI GCGATG 1 cut(s) 144
Cac8I GCNNGC 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 335
CviAII CATG 2 cut(s) 76, 309
CviJI RGCY 3 cut(s) 42, 46, 170
CviKI_1 RGCY 3 cut(s) 42, 46, 170
DdeI CTNAG 1 cut(s) 60
DpnI GATC 1 cut(s) 153
DpnII GATC 1 cut(s) 151
Eam1104I CTCTTC 2 cut(s) 17, 291
EarI CTCTTC 2 cut(s) 17, 291
Ecl136II GAGCTC 1 cut(s) 170
Eco130I CCWWGG 1 cut(s) 274
Eco24I GRGCYC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 335
Eco53kI GAGCTC 1 cut(s) 170
Eco88I CYCGRG 1 cut(s) 227
EcoICRI GAGCTC 1 cut(s) 170
EcoT14I CCWWGG 1 cut(s) 274
EcoT22I ATGCAT 1 cut(s) 355
EcoT38I GRGCYC 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 2 cut(s) 79, 312
FaiI YATR 8 cut(s) 77, 89, 245, 270, 287, 310, 351, 355
FatI CATG 2 cut(s) 75, 308
Fnu4HI GCNGC 1 cut(s) 237
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 1 cut(s) 237
GluI GCNGC 1 cut(s) 237
Hin1II CATG 2 cut(s) 79, 312
HinfI GANTC 5 cut(s) 62, 163, 230, 289, 323
HphI GGTGA 3 cut(s) 172, 213, 290
Hpy188I TCNGA 3 cut(s) 61, 67, 186
Hpy188III TCNNGA 2 cut(s) 293, 327
HpyCH4IV ACGT 1 cut(s) 109
HpyCH4V TGCA 5 cut(s) 113, 176, 239, 255, 353
HpyF10VI GCNNNNNNNGC 1 cut(s) 48
HpyF3I CTNAG 1 cut(s) 60
HpySE526I ACGT 1 cut(s) 109
Hsp92II CATG 2 cut(s) 79, 312
Kzo9I GATC 1 cut(s) 151
LmnI GCTCC 1 cut(s) 56
LpnPI CCDG 1 cut(s) 28
Lsp1109I GCAGC 1 cut(s) 223
MaeII ACGT 1 cut(s) 109
MaeIII GTNAC 1 cut(s) 219
MalI GATC 1 cut(s) 153
MboI GATC 1 cut(s) 151
MboII GAAGA 5 cut(s) 17, 34, 44, 95, 308
MhlI GDGCHC 1 cut(s) 172
MluCI AATT 1 cut(s) 214
MlyI GAGTC 3 cut(s) 71, 239, 298
MnlI CCTC 2 cut(s) 18, 65
Mph1103I ATGCAT 1 cut(s) 355
MseI TTAA 1 cut(s) 263
MwoI GCNNNNNNNGC 1 cut(s) 48
NdeII GATC 1 cut(s) 151
NlaIII CATG 2 cut(s) 79, 312
NlaIV GGNNCC 1 cut(s) 336
NmuCI GTSAC 1 cut(s) 219
NsiI ATGCAT 1 cut(s) 355
NspI RCATGY 2 cut(s) 79, 312
PciI ACATGT 1 cut(s) 75
PfeI GAWTC 2 cut(s) 163, 323
PkrI GCNGC 1 cut(s) 238
PleI GAGTC 3 cut(s) 70, 238, 297
PpsI GAGTC 3 cut(s) 70, 238, 297
PscI ACATGT 1 cut(s) 75
Psp124BI GAGCTC 1 cut(s) 172
PspN4I GGNNCC 1 cut(s) 336
PspPI GGNCC 1 cut(s) 335
SacI GAGCTC 1 cut(s) 172
SaqAI TTAA 1 cut(s) 263
SatI GCNGC 1 cut(s) 237
Sau3AI GATC 1 cut(s) 151
Sau96I GGNCC 1 cut(s) 335
SchI GAGTC 3 cut(s) 71, 239, 298
SduI GDGCHC 1 cut(s) 172
SetI ASST 8 cut(s) 12, 44, 112, 162, 172, 210, 276, 350
SinI GGWCC 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 1 cut(s) 214
SsiI CCGC 1 cut(s) 224
SstI GAGCTC 1 cut(s) 172
StyI CCWWGG 1 cut(s) 274
TaiI ACGT 1 cut(s) 112
TaqI TCGA 2 cut(s) 166, 326
TasI AATT 1 cut(s) 214
TfiI GAWTC 2 cut(s) 163, 323
Tru1I TTAA 1 cut(s) 263
Tru9I TTAA 1 cut(s) 263
TseFI GTSAC 1 cut(s) 219
TseI GCWGC 1 cut(s) 236
Tsp45I GTSAC 1 cut(s) 219
TspDTI ATGAA 1 cut(s) 147
VpaK11BI GGWCC 1 cut(s) 335
XceI RCATGY 2 cut(s) 79, 312
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.