Rorug05G0048700

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002942
Physical Location & Seq
Forward (+)
4267114 .. 4270263
3150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0048700.1

Sequence Viewer

Length: 996 bp
ATGAATGCTGTTCCGCTGACTCTGTTTCCATTTCTGGTGTGGGATGCAACTGCTTTTCTTTCTGAAATTGTATCGGAATACTGTACTGGTGCCAAGGCATTGGTGCCAGAACAGGAAATTTCTACAGGAGAGCAATGTGGGCATGTGGCTACTGTGGAGCCTGGACTTGGTAAATGTGGTACCGGAAAACAGCCGTTTCAGCTTCCTGATTTCATTGCTGCAACTGGAATTGAGAAAATTAGACAGGCATACATTGAAAAAGAAGATAGTAAGAAGACAAAGCAAGTGCAACGAGAATGTATGCAGCCAAAGATGGGCAAAACAGATATTGATTATCAGGTTCTTCATGATGCCTTTTTCAAGTACCAAACTATGCCAGAGCTCACAACACTTGGTGATCTGTACCATGAAGGGAAAGAGTTTGAGAGATATGGTCCCCCCTCATTATATCCACATTTGAAAATCCCTGGACTGAATGCTCCCATCCCCACCGGAGCTAGCTTTGGTAATCATCAACAAGATCAGCCTAATTATGAGGAAGAGCCAGTTGATAAGACAAAGCATTGGGGTGATTTGGAGAGAGAGGAAGAAGAGGAAGAACCGATGGAGGGCAAAGATCTAGAAGATGGTGTTGAATCTGTAGATAGCCTTTCAAGTACTCCCACTGGCAATGAGAAACCAGATGTCATTGACCTTCACAAGCAGCAGAGAAAGGAGCCTGAAAGACCTTTATACCAAGTCAGAAAGCATATAAAGTGGATGTTACTACAACCTGAAGAGTTAGAAGCTATGGAGAACGTCTTGCCTGAAAAGTATGAACAAGCAAGGGGGGAGAAGAAGCTGCGAAGTCAGCGAGAGGAATTCAGTGACATGGTTGCGGAGGTGTCGAAGAAGAGGAAACGTAAGATGCCATACATGAGAGATCTGAGTTCTATCAAGTTTTGCAAGAAGCCAAGCAGATTACAGGTGACTGTTACAAGAATAGCGGGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

331

Amino Acids

37.9

Weight (kDa)

5.49

Isoelectric Point (pI)

55.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF382 PF04037 58 - 140 9.9e-31 Domain of unknown function (DUF382)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 179
AccB1I GGYRCC 3 cut(s) 89, 103, 179
AciI CCGC 3 cut(s) 14, 878, 986
AcsI RAATTY 2 cut(s) 117, 860
AcuI CTGAAG 1 cut(s) 795
AdeI CACNNNGTG 1 cut(s) 395
AfaI GTAC 5 cut(s) 85, 181, 365, 404, 658
AfiI CCNNNNNNNGG 3 cut(s) 167, 314, 608
AgsI TTSAA 5 cut(s) 257, 361, 460, 635, 654
AjnI CCWGG 2 cut(s) 160, 466
AluBI AGCT 6 cut(s) 202, 382, 497, 501, 788, 841
AluI AGCT 6 cut(s) 202, 382, 497, 501, 788, 841
Alw21I GWGCWC 1 cut(s) 384
ApeKI GCWGC 4 cut(s) 218, 304, 703, 841
ApoI RAATTY 2 cut(s) 117, 860
Asp718I GGTACC 1 cut(s) 179
AspS9I GGNCC 1 cut(s) 434
AsuHPI GGTGA 3 cut(s) 407, 581, 979
AsuNHI GCTAGC 1 cut(s) 497
AvaII GGWCC 1 cut(s) 434
BanI GGYRCC 3 cut(s) 89, 103, 179
BanII GRGCYC 1 cut(s) 384
BbsI GAAGAC 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 384
BbvI GCAGC 4 cut(s) 205, 316, 715, 828
BccI CCATC 4 cut(s) 307, 491, 598, 620
BceAI ACGGC 1 cut(s) 178
BciT130I CCWGG 2 cut(s) 162, 468
BfaI CTAG 2 cut(s) 498, 620
BfmI CTRYAG 2 cut(s) 123, 639
BglII AGATCT 2 cut(s) 616, 922
BisI GCNGC 4 cut(s) 219, 305, 704, 842
BlsI GCNGC 4 cut(s) 220, 306, 705, 843
BmcAI AGTACT 1 cut(s) 658
Bme1390I CCNGG 2 cut(s) 162, 468
Bme18I GGWCC 1 cut(s) 434
BmgT120I GGNCC 1 cut(s) 434
BmiI GGNNCC 6 cut(s) 91, 105, 159, 181, 436, 717
BmrFI CCNGG 2 cut(s) 162, 468
BmsI GCATC 3 cut(s) 34, 340, 897
BmtI GCTAGC 1 cut(s) 501
BpiI GAAGAC 1 cut(s) 281
BsaJI CCNNGG 2 cut(s) 93, 466
BsaWI WCCGGW 2 cut(s) 182, 491
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 314, 608
Bse1I ACTGG 4 cut(s) 91, 229, 545, 670
Bse3DI GCAATG 3 cut(s) 140, 213, 676
BseBI CCWGG 2 cut(s) 162, 468
BseDI CCNNGG 2 cut(s) 93, 466
BseGI GGATG 3 cut(s) 49, 483, 765
BseLI CCNNNNNNNGG 3 cut(s) 167, 314, 608
BseMI GCAATG 3 cut(s) 140, 213, 676
BseMII CTCAG 1 cut(s) 917
BseNI ACTGG 4 cut(s) 91, 229, 545, 670
BseXI GCAGC 4 cut(s) 205, 316, 715, 828
BshNI GGYRCC 3 cut(s) 89, 103, 179
BsiHKAI GWGCWC 1 cut(s) 384
BsiSI CCGG 2 cut(s) 183, 492
BslFI GGGAC 1 cut(s) 420
BslI CCNNNNNNNGG 3 cut(s) 167, 314, 608
BsmFI GGGAC 1 cut(s) 420
BsmI GAATGC 2 cut(s) 10, 481
Bsp1286I GDGCHC 1 cut(s) 384
Bsp143I GATC 4 cut(s) 397, 520, 616, 922
BspACI CCGC 3 cut(s) 14, 878, 986
BspCNI CTCAG 1 cut(s) 918
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 6 cut(s) 91, 105, 159, 181, 436, 717
BspOI GCTAGC 1 cut(s) 501
BspQI GCTCTTC 1 cut(s) 534
BspT107I GGYRCC 3 cut(s) 89, 103, 179
BsrDI GCAATG 3 cut(s) 140, 213, 676
BsrI ACTGG 4 cut(s) 91, 229, 545, 670
BssECI CCNNGG 2 cut(s) 93, 466
BssMI GATC 4 cut(s) 397, 520, 616, 922
BssT1I CCWWGG 1 cut(s) 93
Bst2UI CCWGG 2 cut(s) 162, 468
Bst4CI ACNGT 3 cut(s) 83, 154, 973
Bst6I CTCTTC 4 cut(s) 534, 585, 771, 887
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 1 cut(s) 926
BstF5I GGATG 3 cut(s) 49, 483, 765
BstKTI GATC 4 cut(s) 400, 523, 619, 925
BstMBI GATC 4 cut(s) 397, 520, 616, 922
BstMWI GCNNNNNNNGC 3 cut(s) 139, 199, 850
BstNI CCWGG 2 cut(s) 162, 468
BstNSI RCATGY 1 cut(s) 146
BstSCI CCNGG 2 cut(s) 160, 466
BstSFI CTRYAG 2 cut(s) 123, 639
BstV1I GCAGC 4 cut(s) 205, 316, 715, 828
BstV2I GAAGAC 1 cut(s) 281
BstX2I RGATCY 2 cut(s) 616, 922
BstXI CCANNNNNNTGG 1 cut(s) 100
BstYI RGATCY 2 cut(s) 616, 922
BtsCI GGATG 3 cut(s) 49, 483, 765
BtsIMutI CAGTG 2 cut(s) 663, 871
Cac8I GCNNGC 1 cut(s) 499
CciI TCATGA 1 cut(s) 346
Cfr13I GGNCC 1 cut(s) 434
Csp6I GTAC 5 cut(s) 84, 180, 364, 403, 657
CviAII CATG 5 cut(s) 143, 347, 407, 871, 916
CviQI GTAC 5 cut(s) 84, 180, 364, 403, 657
DdeI CTNAG 1 cut(s) 926
DpnI GATC 4 cut(s) 399, 522, 618, 924
DpnII GATC 4 cut(s) 397, 520, 616, 922
DraIII CACNNNGTG 1 cut(s) 395
Eam1104I CTCTTC 4 cut(s) 534, 585, 771, 887
EarI CTCTTC 4 cut(s) 534, 585, 771, 887
Ecl136II GAGCTC 1 cut(s) 382
Eco130I CCWWGG 1 cut(s) 93
Eco24I GRGCYC 1 cut(s) 384
Eco47I GGWCC 1 cut(s) 434
Eco53kI GAGCTC 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 795
EcoICRI GAGCTC 1 cut(s) 382
EcoRI GAATTC 1 cut(s) 860
EcoRII CCWGG 2 cut(s) 160, 466
EcoT14I CCWWGG 1 cut(s) 93
EcoT38I GRGCYC 1 cut(s) 384
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 5 cut(s) 146, 350, 410, 874, 919
FaqI GGGAC 1 cut(s) 420
FatI CATG 5 cut(s) 142, 346, 406, 870, 915
FauI CCCGC 1 cut(s) 979
Fnu4HI GCNGC 4 cut(s) 219, 305, 704, 842
FokI GGATG 3 cut(s) 56, 470, 772
FriOI GRGCYC 1 cut(s) 384
Fsp4HI GCNGC 4 cut(s) 219, 305, 704, 842
FspBI CTAG 2 cut(s) 498, 620
GluI GCNGC 4 cut(s) 219, 305, 704, 842
HapII CCGG 2 cut(s) 183, 492
Hin1II CATG 5 cut(s) 146, 350, 410, 874, 919
HinfI GANTC 2 cut(s) 19, 635
HpaII CCGG 2 cut(s) 183, 492
HphI GGTGA 3 cut(s) 407, 581, 979
Hpy188I TCNGA 4 cut(s) 64, 76, 743, 927
Hpy188III TCNNGA 3 cut(s) 206, 347, 620
HpyAV CCTTC 2 cut(s) 404, 704
HpyCH4III ACNGT 3 cut(s) 83, 154, 973
HpyCH4IV ACGT 2 cut(s) 798, 901
HpyCH4V TGCA 5 cut(s) 47, 221, 289, 304, 945
HpyF10VI GCNNNNNNNGC 3 cut(s) 139, 199, 850
HpyF3I CTNAG 1 cut(s) 926
HpySE526I ACGT 2 cut(s) 798, 901
Hsp92II CATG 5 cut(s) 146, 350, 410, 874, 919
KpnI GGTACC 1 cut(s) 183
Kzo9I GATC 4 cut(s) 397, 520, 616, 922
LguI GCTCTTC 1 cut(s) 534
LmnI GCTCC 4 cut(s) 157, 484, 494, 715
Lsp1109I GCAGC 4 cut(s) 205, 316, 715, 828
LweI GCATC 3 cut(s) 34, 340, 897
MaeI CTAG 2 cut(s) 498, 620
MaeII ACGT 2 cut(s) 798, 901
MaeIII GTNAC 4 cut(s) 762, 866, 967, 973
MalI GATC 4 cut(s) 399, 522, 618, 924
MboI GATC 4 cut(s) 397, 520, 616, 922
MflI RGATCY 2 cut(s) 616, 922
MhlI GDGCHC 1 cut(s) 384
MluCI AATT 6 cut(s) 66, 117, 228, 237, 529, 860
MlyI GAGTC 1 cut(s) 13
MnlI CCTC 8 cut(s) 451, 529, 577, 586, 601, 850, 874, 888
MslI CAYNNNNRTG 1 cut(s) 567
MspA1I CMGCKG 1 cut(s) 16
MspI CCGG 2 cut(s) 183, 492
MspR9I CCNGG 2 cut(s) 162, 468
Mva1269I GAATGC 2 cut(s) 10, 481
MvaI CCWGG 2 cut(s) 162, 468
MwoI GCNNNNNNNGC 3 cut(s) 139, 199, 850
NdeII GATC 4 cut(s) 397, 520, 616, 922
NheI GCTAGC 1 cut(s) 497
NlaIII CATG 5 cut(s) 146, 350, 410, 874, 919
NlaIV GGNNCC 6 cut(s) 91, 105, 159, 181, 436, 717
NmuCI GTSAC 2 cut(s) 866, 967
NspI RCATGY 1 cut(s) 146
PagI TCATGA 1 cut(s) 346
PciSI GCTCTTC 1 cut(s) 534
PctI GAATGC 2 cut(s) 10, 481
PfeI GAWTC 1 cut(s) 635
PkrI GCNGC 4 cut(s) 220, 306, 705, 843
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 384
Psp6I CCWGG 2 cut(s) 160, 466
PspGI CCWGG 2 cut(s) 160, 466
PspN4I GGNNCC 6 cut(s) 91, 105, 159, 181, 436, 717
PspPI GGNCC 1 cut(s) 434
PsuI RGATCY 2 cut(s) 616, 922
RsaI GTAC 5 cut(s) 85, 181, 365, 404, 658
RsaNI GTAC 5 cut(s) 84, 180, 364, 403, 657
RseI CAYNNNNRTG 1 cut(s) 567
SacI GAGCTC 1 cut(s) 384
SapI GCTCTTC 1 cut(s) 534
SatI GCNGC 4 cut(s) 219, 305, 704, 842
Sau3AI GATC 4 cut(s) 397, 520, 616, 922
Sau96I GGNCC 1 cut(s) 434
ScaI AGTACT 1 cut(s) 658
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 2 cut(s) 162, 468
SduI GDGCHC 1 cut(s) 384
SfaNI GCATC 3 cut(s) 34, 340, 897
SfcI CTRYAG 2 cut(s) 123, 639
SinI GGWCC 1 cut(s) 434
SmiMI CAYNNNNRTG 1 cut(s) 567
Sse9I AATT 6 cut(s) 66, 117, 228, 237, 529, 860
SsiI CCGC 3 cut(s) 14, 878, 986
SspMI CTAG 2 cut(s) 498, 620
SstI GAGCTC 1 cut(s) 384
StyD4I CCNGG 2 cut(s) 160, 466
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 3 cut(s) 83, 154, 973
TaiI ACGT 2 cut(s) 801, 904
TaqI TCGA 1 cut(s) 887
TasI AATT 6 cut(s) 66, 117, 228, 237, 529, 860
TatI WGTACW 2 cut(s) 83, 656
TfiI GAWTC 1 cut(s) 635
TscAI CASTG 2 cut(s) 670, 871
TseFI GTSAC 2 cut(s) 866, 967
TseI GCWGC 4 cut(s) 218, 304, 703, 841
Tsp45I GTSAC 2 cut(s) 866, 967
TspDTI ATGAA 5 cut(s) 17, 202, 335, 423, 831
TspRI CASTG 2 cut(s) 670, 871
VpaK11BI GGWCC 1 cut(s) 434
XapI RAATTY 2 cut(s) 117, 860
XbaI TCTAGA 1 cut(s) 619
XceI RCATGY 1 cut(s) 146
XcmI CCANNNNNNNNNTGG 1 cut(s) 36
XspI CTAG 2 cut(s) 498, 620
ZrmI AGTACT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.