Rorug05G0048900

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002942
Physical Location & Seq
Reverse (-)
4294350 .. 4295485
1136 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0048900.1

Sequence Viewer

Length: 972 bp
ATGCCTTTGCCTCTCCAATCCAAGCCCAATCTCAATCCCAAGAAATTCGAATTGCTTCAACAGACCGTCCAGACCTTTAAGCGCTTGCAGCGAGGTCCTTTGCTCACTCCCGAGGCTCCGGTGGCGGAGGAGGACGAGGAAGACAGTAATGATGATGATGATGATGATTCCATTTCCACAGACCGTCAGTTACAATCTTTCTCTCTCTTCCATTTCCGGTTTTCTCGCGGGTCCTCTAACGGCCGCGCCATGGTGGCTCCGGAACCCGAGGATGAGAAAAGCTGGCAGGAGCTACTCAGAAAGATGCTGCCTTCCGGCGCACCGTTGCCGGACGATGACCATCTCGACTACTCCATCGCAGTTGAGTACGAAGGCCCTCCGCTGCCCTACGAAGTCCCCAAGGTTGATCCAGTCGACATCGATTCGCTTCACAGCGCTGCTTCCGGAGCTTCAGGTTCGGTTTCGGAGCTTTCTTCGATTCCGGTGGCCGAGCCGGTCGATCGGGACGCCTCTCGGTTCAGCCGGATCAGGAACGGCGACGCCGCAAACGAAACGCAGACTTCGACGTCGTTTTCGGTAGGTTTCAGCTCTGAATTTTCAGCTCAGGACTCGAACTCGGAGCCAAAACCGGTCGAGGACGAAGCCGGAGGAGGAGGAAAGAGAGCAGCGGTCGTGACTTTCAACACGCCGAGAGAATCGGAGACGGAAGACGACGGCGACCGGTCCTCGTCGCCGCAATCGGAGCCAGTGGGATCGCCGGTTGCTTGGGCCTCGCCGTTGAAGGTAGCGAGTAAGAGAGGGATTTGCAGCAGGTGTGGAAAAGGGAACAGATTGAAGGAGAGGGAGTGGTGCTTGGTGTGTGACTCAAAGTACTGTAGTAACTGTTTGCTTAAAGCTATGGGATCAATGCCGGAGGGGCGAAAATGTGTGAGCTGCATTGGGCAGCCTATAAATGTGTTCATGGTCTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

323

Amino Acids

35.15

Weight (kDa)

4.67

Isoelectric Point (pI)

59.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 801
AasI GACNNNNNNGTC 1 cut(s) 565
AatII GACGTC 1 cut(s) 569
Acc36I ACCTGC 1 cut(s) 801
AccI GTMKAC 1 cut(s) 414
AccII CGCG 2 cut(s) 228, 246
AccIII TCCGGA 2 cut(s) 259, 443
AciI CCGC 7 cut(s) 125, 228, 244, 380, 543, 668, 734
AclWI GGATC 4 cut(s) 401, 533, 760, 910
AcoI YGGCCR 2 cut(s) 241, 486
AcsI RAATTY 2 cut(s) 44, 593
AcuI CTGAAG 1 cut(s) 435
AcyI GRCGYC 3 cut(s) 507, 540, 566
AfaI GTAC 2 cut(s) 368, 872
AfeI AGCGCT 2 cut(s) 83, 436
AfiI CCNNNNNNNGG 1 cut(s) 250
AgeI ACCGGT 2 cut(s) 628, 720
AgsI TTSAA 4 cut(s) 59, 682, 781, 835
AluBI AGCT 8 cut(s) 282, 292, 449, 469, 588, 602, 896, 933
AluI AGCT 8 cut(s) 282, 292, 449, 469, 588, 602, 896, 933
Alw26I GTCTC 1 cut(s) 695
AlwI GGATC 4 cut(s) 401, 533, 760, 910
Ama87I CYCGRG 2 cut(s) 110, 266
Aor13HI TCCGGA 2 cut(s) 259, 443
Aor51HI AGCGCT 2 cut(s) 83, 436
AoxI GGCC 4 cut(s) 241, 373, 486, 768
ApeKI GCWGC 8 cut(s) 88, 307, 382, 437, 665, 807, 933, 943
ApoI RAATTY 2 cut(s) 44, 593
ArsI GACNNNNNNTTYG 2 cut(s) 556, 588
AsiGI ACCGGT 2 cut(s) 628, 720
Asp700I GAANNNNTTC 1 cut(s) 54
AspLEI GCGC 4 cut(s) 84, 248, 320, 437
AspS9I GGNCC 5 cut(s) 95, 231, 374, 723, 768
AsuII TTCGAA 1 cut(s) 48
AvaI CYCGRG 2 cut(s) 110, 266
AvaII GGWCC 3 cut(s) 95, 231, 723
BbsI GAAGAC 2 cut(s) 147, 714
BbvI GCAGC 8 cut(s) 100, 294, 369, 424, 677, 819, 920, 955
BccI CCATC 2 cut(s) 348, 362
BceAI ACGGC 4 cut(s) 256, 550, 730, 760
BcoDI GTCTC 1 cut(s) 695
BfmI CTRYAG 1 cut(s) 874
BfoI RGCGCY 2 cut(s) 85, 438
BfuAI ACCTGC 1 cut(s) 801
BglI GCCNNNNNGGC 2 cut(s) 254, 916
BmcAI AGTACT 1 cut(s) 872
Bme18I GGWCC 3 cut(s) 95, 231, 723
BmeT110I CYCGRG 2 cut(s) 110, 266
BmgT120I GGNCC 5 cut(s) 95, 231, 374, 723, 768
BmiI GGNNCC 6 cut(s) 117, 232, 258, 264, 621, 744
BmsI GCATC 1 cut(s) 294
BpiI GAAGAC 2 cut(s) 147, 714
Bpu10I CCTNAGC 1 cut(s) 603
Bpu14I TTCGAA 1 cut(s) 48
Bsa29I ATCGAT 1 cut(s) 420
BsaBI GATNNNNATC 1 cut(s) 339
BsaHI GRCGYC 3 cut(s) 507, 540, 566
BsaJI CCNNGG 4 cut(s) 111, 249, 267, 399
BsaWI WCCGGW 7 cut(s) 118, 216, 259, 443, 481, 628, 720
BsaXI ACNNNNNCTCC 2 cut(s) 830, 860
Bsc4I CCNNNNNNNGG 1 cut(s) 250
Bse118I RCCGGY 4 cut(s) 493, 628, 720, 757
Bse1I ACTGG 2 cut(s) 410, 746
Bse8I GATNNNNATC 1 cut(s) 339
BseAI TCCGGA 2 cut(s) 259, 443
BseCI ATCGAT 1 cut(s) 420
BseDI CCNNGG 4 cut(s) 111, 249, 267, 399
BseGI GGATG 1 cut(s) 277
BseJI GATNNNNATC 1 cut(s) 339
BseLI CCNNNNNNNGG 1 cut(s) 250
BseMII CTCAG 2 cut(s) 310, 617
BseNI ACTGG 2 cut(s) 410, 746
BseRI GAGGAG 3 cut(s) 143, 663, 666
BseX3I CGGCCG 1 cut(s) 241
BseXI GCAGC 8 cut(s) 100, 294, 369, 424, 677, 819, 920, 955
Bsh1236I CGCG 2 cut(s) 228, 246
Bsh1285I CGRYCG 6 cut(s) 244, 498, 502, 633, 672, 721
BshFI GGCC 4 cut(s) 243, 375, 488, 770
BshTI ACCGGT 2 cut(s) 628, 720
BshVI ATCGAT 1 cut(s) 420
BsiEI CGRYCG 6 cut(s) 244, 498, 502, 633, 672, 721
BsiHKCI CYCGRG 2 cut(s) 110, 266
BslFI GGGAC 2 cut(s) 380, 518
BslI CCNNNNNNNGG 1 cut(s) 250
BsmAI GTCTC 1 cut(s) 695
BsmBI CGTCTC 1 cut(s) 695
BsmFI GGGAC 2 cut(s) 380, 518
BsnI GGCC 4 cut(s) 243, 375, 488, 770
BsoBI CYCGRG 2 cut(s) 110, 266
Bsp119I TTCGAA 1 cut(s) 48
Bsp13I TCCGGA 2 cut(s) 259, 443
Bsp143I GATC 5 cut(s) 406, 499, 525, 752, 902
Bsp19I CCATGG 1 cut(s) 249
BspACI CCGC 7 cut(s) 125, 228, 244, 380, 543, 668, 734
BspANI GGCC 4 cut(s) 243, 375, 488, 770
BspCNI CTCAG 2 cut(s) 309, 616
BspDI ATCGAT 1 cut(s) 420
BspEI TCCGGA 2 cut(s) 259, 443
BspFNI CGCG 2 cut(s) 228, 246
BspLI GGNNCC 6 cut(s) 117, 232, 258, 264, 621, 744
BspMI ACCTGC 1 cut(s) 801
BspPI GGATC 4 cut(s) 401, 533, 760, 910
BspT104I TTCGAA 1 cut(s) 48
BsrFI RCCGGY 4 cut(s) 493, 628, 720, 757
BsrI ACTGG 2 cut(s) 410, 746
BssAI RCCGGY 4 cut(s) 493, 628, 720, 757
BssECI CCNNGG 4 cut(s) 111, 249, 267, 399
BssMI GATC 5 cut(s) 406, 499, 525, 752, 902
BssNI GRCGYC 3 cut(s) 507, 540, 566
BssT1I CCWWGG 2 cut(s) 249, 399
Bst4CI ACNGT 6 cut(s) 67, 146, 185, 324, 875, 884
Bst6I CTCTTC 1 cut(s) 212
BstACI GRCGYC 3 cut(s) 507, 540, 566
BstBI TTCGAA 1 cut(s) 48
BstC8I GCNNGC 2 cut(s) 86, 284
BstDEI CTNAG 2 cut(s) 296, 603
BstDSI CCRYGG 1 cut(s) 249
BstF5I GGATG 1 cut(s) 277
BstFNI CGCG 2 cut(s) 228, 246
BstH2I RGCGCY 2 cut(s) 85, 438
BstHHI GCGC 4 cut(s) 84, 248, 320, 437
BstKTI GATC 5 cut(s) 409, 502, 528, 755, 905
BstMAI GTCTC 1 cut(s) 695
BstMBI GATC 5 cut(s) 406, 499, 525, 752, 902
BstMCI CGRYCG 6 cut(s) 244, 498, 502, 633, 672, 721
BstMWI GCNNNNNNNGC 6 cut(s) 88, 122, 254, 446, 742, 916
BstSFI CTRYAG 1 cut(s) 874
BstUI CGCG 2 cut(s) 228, 246
BstV1I GCAGC 8 cut(s) 100, 294, 369, 424, 677, 819, 920, 955
BstV2I GAAGAC 2 cut(s) 147, 714
BstZI CGGCCG 1 cut(s) 241
Bsu15I ATCGAT 1 cut(s) 420
BsuRI GGCC 4 cut(s) 243, 375, 488, 770
BsuTUI ATCGAT 1 cut(s) 420
BtgI CCRYGG 1 cut(s) 249
BtgZI GCGATG 1 cut(s) 340
BtsCI GGATG 1 cut(s) 277
BtsIMutI CAGTG 1 cut(s) 753
BveI ACCTGC 1 cut(s) 801
Cac8I GCNNGC 2 cut(s) 86, 284
CfoI GCGC 4 cut(s) 84, 248, 320, 437
Cfr10I RCCGGY 4 cut(s) 493, 628, 720, 757
Cfr13I GGNCC 5 cut(s) 95, 231, 374, 723, 768
ClaI ATCGAT 1 cut(s) 420
CseI GACGC 2 cut(s) 515, 548
Csp6I GTAC 2 cut(s) 367, 871
CspAI ACCGGT 2 cut(s) 628, 720
CviAII CATG 2 cut(s) 250, 961
CviQI GTAC 2 cut(s) 367, 871
DdeI CTNAG 2 cut(s) 296, 603
DpnI GATC 5 cut(s) 408, 501, 527, 754, 904
DpnII GATC 5 cut(s) 406, 499, 525, 752, 902
DrdI GACNNNNNNGTC 1 cut(s) 565
DseDI GACNNNNNNGTC 1 cut(s) 565
EaeI YGGCCR 2 cut(s) 241, 486
EagI CGGCCG 1 cut(s) 241
Eam1104I CTCTTC 1 cut(s) 212
EarI CTCTTC 1 cut(s) 212
EciI GGCGGA 1 cut(s) 140
EclXI CGGCCG 1 cut(s) 241
Eco130I CCWWGG 2 cut(s) 249, 399
Eco47I GGWCC 3 cut(s) 95, 231, 723
Eco47III AGCGCT 2 cut(s) 83, 436
Eco52I CGGCCG 1 cut(s) 241
Eco57I CTGAAG 1 cut(s) 435
Eco88I CYCGRG 2 cut(s) 110, 266
EcoO109I RGGNCCY 3 cut(s) 95, 231, 374
EcoT14I CCWWGG 2 cut(s) 249, 399
ErhI CCWWGG 2 cut(s) 249, 399
Esp3I CGTCTC 1 cut(s) 695
FaeI CATG 2 cut(s) 253, 964
FaiI YATR 4 cut(s) 251, 899, 950, 962
FaqI GGGAC 2 cut(s) 380, 518
FatI CATG 2 cut(s) 249, 960
FauI CCCGC 1 cut(s) 221
FblI GTMKAC 1 cut(s) 414
FokI GGATG 1 cut(s) 284
GlaI GCGC 4 cut(s) 83, 247, 319, 436
HaeII RGCGCY 2 cut(s) 85, 438
HaeIII GGCC 4 cut(s) 243, 375, 488, 770
HgaI GACGC 2 cut(s) 515, 548
HhaI GCGC 4 cut(s) 84, 248, 320, 437
Hin1I GRCGYC 3 cut(s) 507, 540, 566
Hin1II CATG 2 cut(s) 253, 964
Hin6I GCGC 4 cut(s) 82, 246, 318, 435
HinP1I GCGC 4 cut(s) 82, 246, 318, 435
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HinfI GANTC 6 cut(s) 167, 422, 478, 608, 695, 863
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 6 cut(s) 299, 466, 592, 619, 700, 742
Hpy188III TCNNGA 9 cut(s) 70, 110, 260, 344, 444, 503, 529, 605, 673
Hpy8I GTNNAC 1 cut(s) 415
Hpy99I CGWCG 5 cut(s) 542, 568, 571, 716, 733
HpyAV CCTTC 4 cut(s) 321, 365, 775, 829
HpyCH4III ACNGT 6 cut(s) 67, 146, 185, 324, 875, 884
HpyCH4IV ACGT 1 cut(s) 566
HpyCH4V TGCA 3 cut(s) 88, 807, 936
HpyF10VI GCNNNNNNNGC 6 cut(s) 88, 122, 254, 446, 742, 916
HpyF3I CTNAG 2 cut(s) 296, 603
HpySE526I ACGT 1 cut(s) 566
Hsp92I GRCGYC 3 cut(s) 507, 540, 566
Hsp92II CATG 2 cut(s) 253, 964
HspAI GCGC 4 cut(s) 82, 246, 318, 435
Kpn2I TCCGGA 2 cut(s) 259, 443
Kzo9I GATC 5 cut(s) 406, 499, 525, 752, 902
LmnI GCTCC 7 cut(s) 121, 262, 289, 446, 466, 619, 742
Lsp1109I GCAGC 8 cut(s) 100, 294, 369, 424, 677, 819, 920, 955
LweI GCATC 1 cut(s) 294
MaeII ACGT 1 cut(s) 566
MaeIII GTNAC 4 cut(s) 189, 673, 860, 878
MalI GATC 5 cut(s) 408, 501, 527, 754, 904
MboI GATC 5 cut(s) 406, 499, 525, 752, 902
MboII GAAGA 4 cut(s) 152, 199, 465, 719
MluCI AATT 3 cut(s) 44, 50, 593
MlyI GAGTC 2 cut(s) 602, 857
MroI TCCGGA 2 cut(s) 259, 443
MroXI GAANNNNTTC 1 cut(s) 54
MseI TTAA 2 cut(s) 78, 891
MspA1I CMGCKG 2 cut(s) 382, 668
MvnI CGCG 2 cut(s) 228, 246
MwoI GCNNNNNNNGC 6 cut(s) 88, 122, 254, 446, 742, 916
NcoI CCATGG 1 cut(s) 249
NdeII GATC 5 cut(s) 406, 499, 525, 752, 902
NlaIII CATG 2 cut(s) 253, 964
NlaIV GGNNCC 6 cut(s) 117, 232, 258, 264, 621, 744
NmeAIII GCCGAG 2 cut(s) 514, 714
NmuCI GTSAC 2 cut(s) 673, 860
NspV TTCGAA 1 cut(s) 48
PaqCI CACCTGC 1 cut(s) 801
PcsI WCGNNNNNNNCGW 2 cut(s) 546, 560
PdmI GAANNNNTTC 1 cut(s) 54
PfeI GAWTC 4 cut(s) 167, 422, 478, 695
PinAI ACCGGT 2 cut(s) 628, 720
Ple19I CGATCG 1 cut(s) 502
PleI GAGTC 2 cut(s) 602, 857
PpsI GAGTC 2 cut(s) 602, 857
PpuMI RGGWCCY 2 cut(s) 95, 231
Psp5II RGGWCCY 2 cut(s) 95, 231
PspN4I GGNNCC 6 cut(s) 117, 232, 258, 264, 621, 744
PspPI GGNCC 5 cut(s) 95, 231, 374, 723, 768
PspPPI RGGWCCY 2 cut(s) 95, 231
PvuI CGATCG 1 cut(s) 502
RsaI GTAC 2 cut(s) 368, 872
RsaNI GTAC 2 cut(s) 367, 871
SalI GTCGAC 1 cut(s) 413
SaqAI TTAA 2 cut(s) 78, 891
Sau3AI GATC 5 cut(s) 406, 499, 525, 752, 902
Sau96I GGNCC 5 cut(s) 95, 231, 374, 723, 768
ScaI AGTACT 1 cut(s) 872
SchI GAGTC 2 cut(s) 602, 857
SfaNI GCATC 1 cut(s) 294
SfcI CTRYAG 1 cut(s) 874
SfuI TTCGAA 1 cut(s) 48
SinI GGWCC 3 cut(s) 95, 231, 723
Sse9I AATT 3 cut(s) 44, 50, 593
SsiI CCGC 7 cut(s) 125, 228, 244, 380, 543, 668, 734
StyI CCWWGG 2 cut(s) 249, 399
TaaI ACNGT 6 cut(s) 67, 146, 185, 324, 875, 884
TaiI ACGT 1 cut(s) 569
TaqI TCGA 9 cut(s) 48, 345, 414, 420, 476, 498, 563, 611, 633
TasI AATT 3 cut(s) 44, 50, 593
TatI WGTACW 1 cut(s) 870
TauI GCSGC 3 cut(s) 246, 545, 736
TfiI GAWTC 4 cut(s) 167, 422, 478, 695
Tru1I TTAA 2 cut(s) 78, 891
Tru9I TTAA 2 cut(s) 78, 891
TscAI CASTG 1 cut(s) 753
TseFI GTSAC 2 cut(s) 673, 860
TseI GCWGC 8 cut(s) 88, 307, 382, 437, 665, 807, 933, 943
Tsp45I GTSAC 2 cut(s) 673, 860
TspDTI ATGAA 1 cut(s) 949
TspGWI ACGGA 1 cut(s) 719
TspRI CASTG 1 cut(s) 753
VpaK11BI GGWCC 3 cut(s) 95, 231, 723
XapI RAATTY 2 cut(s) 44, 593
XmiI GTMKAC 1 cut(s) 414
XmnI GAANNNNTTC 1 cut(s) 54
ZraI GACGTC 1 cut(s) 567
ZrmI AGTACT 1 cut(s) 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.