Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001578

Physical Location & Seq

Genomic Coordinates Forward (+)

33809560 .. 33818296

Gene Structure 8737 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1243800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2097 bp

ATGCGTCTTCTTCTCCATCGGCCTTTCCGCAAGCTCTCCTTCTTATCCTCCTCAACCTCCGGCCTCCTAATGGCCTCCAAGCAGCTGCTCAAGTCCGCTCCTTTTCCAAACCCACTGACCCCTTCCGCACACAGACACCCCTTCCACTCTCGCAGCCTCGTCTTCTCTCCGATTTCCTCGAAACCCGTGTTGAAAATTCGGCCGCTTTGCCTCCGGACTAACGGATATTCTTCTCTCTCCCCTGCCCATGTCGCAGAACCAGTTGAAGTGGAAACCCCAAAAGAAAATGGAATGTCCTCTATGCAAATGAGGATCTTGAAGGAGAAATTGGAGAATCTTGGCATAGACGGTGAAACAATATCTGTACCAGGGCAGTACAACCACTTGATTTGCCCAATGTGCAAAGGTGGCGATTCAGAGGAAAAGAGCTTATCCATGGATATTTCGGAAAATTGGGATCGTGCTATTTGGTGTTGCTTTCGTGGAAAATGTGGGTGGACAGGTAGCACGAGGGCCTTTGTAGGAAACAGGTTGCCACCTGAAACTTCAAAACTTAAGAAAAAAAGGGAAATCACAGAGGAAAGTCTAGGACTGGAACCACTTTGTGAGGAGATTATAGATTATTTTTCAGAACGAATGATATCAAGGGGAACATTGATGAGAAATGCTGTAATGCAAAAAAGTACAGGTGATCAGATTGCTATTGCTTTTCCTTATCGGAGAGACGGAAAGCTTGTTAGTTGCAAGTATCGGGACATCGACAAGAAGTTCTGGCAGGAAAAGGATACTGAAAAGATTTTCTATGGACTGGACGATATAAAGGGAACTAATGATATCATCATTGTTGAAGGCGAAATTGATAAGCTTGCAATGGAAGAAGCTGGCTTTCGTAATTGTGTGAGTGTACCTGATGGTGCTCCTCCAAAGGTTTCCTCAAAGGAGTTGCCACCCGAAGAGAAGGATACAAAGTATCAATATCTGTGGAACTGCAAAGAGTACTTAAAGAAGGCATCACGCATTGTACTTGCCACTGATGGAGATGTTCCTGGTCAAGCCCTAGCTGAAGAGCTTGCACGCCGCCTTGGAAGAGAAAGATGTTGGCGAGTCAAATGGCCAAAGAAAAATGATAAGGAGAACTTCAAAGATGCAAATGAGGTTCTCATGTACTTGGGTTCGGATGTATTGAAGGGAGTAATTGAGGATGCTGAATTATTTCCTATACGTGGATTGTTCAATTTTGCGAATTACTTTGATGAACTCGATGCATATTATCACCGGACTCTTGGATATGAGTATGGTGTCTCAACTGGATGGAAAAATCTGAATGATTTGTACAATGTTGTTCCTGGTGAGTTGACTATAGTAACAGGGGTCCCTAATTCAGGCAAGAGTGAGTGGATTGATGCTCTCTTGTGCAATCTATCTGCAAGTGTTGGATGGAAATTTGTACTTTGCTCTATGGAGAACAAGGTTAGGGAGCATGCACGAAAATTGCTGGAGAAGCACATAAAGAAGCCTTTCTTTGATAAACGTTATGGTGGTTCTGCTGAACGGATGAGTGTTGAAGAATTTGAGCAAGGAAAAAAATGGTTAAATGACACCTTTTACCTCATAAGGTGTGAGGATGATTCTCTTCCAAATATCAGTTGGGTTCTTGATCTTGCACAAGCAGCAGTACTTAGGCATGGGGTGCGTGGACTTGTAATTGATCCTTACAATGAGCTTGATCATCAACGTCCCCCGAACCAGACCGAAACAGAGTATGTGAGTCAGATGCTTACTAAGATCAAGCGGTTTGCTCAGCATCATTGTTGCCATGTTTGGTTTGTGGCGCATCCTAGACAGTTGCACCAGTGGACCGGGGGTCCTCCTAATCTCTATGATATAAGTGGAAGTGCACACTTCATAAATAAATGCGACAATGGAATTGTAATTCATCGTAATCGGGATCCAGAGGCTGGGGAGATTGATCAAGTACAAGTTTGTGTACGGAAGGTACGAAATAAGGTCGCGGGAACCATCGGTGACGCCTTTTTGTCATACGATAGGGCAACTGGTCAGTTTATGGATGTTGAAAATACTCGAAAGAGCACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003697 GO:0003824 GO:0003896 GO:0003899 GO:0004003 GO:0004386 GO:0005488 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006269 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009117 GO:0009165 GO:0009259 GO:0009260 GO:0009987 GO:0016043 GO:0016070 GO:0016462 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0032392 GO:0032508 GO:0032774 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0042623 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044281 GO:0046390 GO:0046483 GO:0051276 GO:0055086 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0090407 GO:0097159 GO:0097747 GO:0140097 GO:0140098 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901363 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DnaB_C Toprim_4

Protein Analysis

699

Amino Acids

79.55

Weight (kDa)

8.26

Isoelectric Point (pI)

34.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Toprim_4	PF13662	278 - 362	1.8e-12	Toprim domain
Toprim	PF01751	279 - 359	6e-09	Toprim domain
Toprim_2	PF13155	280 - 386	3.7e-06	Toprim-like
DnaB_C	PF03796	433 - 688	7.5e-10	DnaB-like helicase C terminal domain
AAA_25	PF13481	447 - 615	1.3e-07	AAA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001578)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30660 AT1G30660 AT1G30660 AT1G30680
fragaria_vesca	FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11220 FvH4_3g11220
malus_domestica	MD05G1265600.v1.1 MD10G1243800.v1.1
prunus_persica	Prupe.4G099700_v2.0.a1 Prupe.4G099700_v2.0.a1 Prupe.4G099700_v2.0.a1 Prupe.4G099700_v2.0.a1 Prupe.4G099900_v2.0.a1 Prupe.4G099900_v2.0.a1
pyrus_communis	pycom05g24970 pycom10g20390
rosa_chinensis	RchiOBHm_Chr5g0018141 RchiOBHm_Chr5g0018171
rosa_laevigata	RLG00000032341 RLG00000032343
rosa_multiflora	Rmu_sc0006594.1_g000001 Rmu_sc0013020.1_g000001 Rmu_sc0013020.1_g000004
rosa_roxburghii	Rroxscaffold_1G00059480 Rroxscaffold_1G00059500
rosa_rugosa	Rorug05G0040700 Rorug05G0040900
rosa_samantha	Rh5AG133500 Rh5AG133800 Rh5BG131900 Rh5BG132300 Rh5CG144200 Rh5CG144500 Rh5DG133900 Rh5DG134200
rosa_wichuraiana	Rw5G011640 Rw5G011690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1958
AccBSI	CCGCTC	1 cut(s)	98
AccII	CGCG	1 cut(s)	2012
AccIII	TCCGGA	1 cut(s)	213
AciI	CCGC	7 cut(s)	28, 96, 126, 203, 1078, 1792, 2012
AclI	AACGTT	1 cut(s)	1531
AclWI	GGATC	5 cut(s)	320, 465, 1703, 1943, 1956
AcoI	YGGCCR	2 cut(s)	200, 1112
AcsI	RAATTY	3 cut(s)	195, 1442, 1568
AcuI	CTGAAG	1 cut(s)	1083
AcyI	GRCGYC	1 cut(s)	2028
AdeI	CACNNNGTG	1 cut(s)	605
AfiI	CCNNNNNNNGG	4 cut(s)	70, 1223, 1382, 1958
AflII	CTTAAG	1 cut(s)	554
AhdI	GACNNNNNGTC	1 cut(s)	1863
AjnI	CCWGG	3 cut(s)	367, 1045, 1345
AjuI	GAANNNNNNNTTGG	2 cut(s)	311, 343
AluBI	AGCT	9 cut(s)	34, 85, 429, 733, 865, 881, 1061, 1069, 1723
AluI	AGCT	9 cut(s)	34, 85, 429, 733, 865, 881, 1061, 1069, 1723
Alw21I	GWGCWC	3 cut(s)	919, 1900, 2093
Alw26I	GTCTC	2 cut(s)	717, 1306
Alw44I	GTGCAC	1 cut(s)	1896
AlwI	GGATC	5 cut(s)	320, 465, 1703, 1943, 1956
AlwNI	CAGNNNCTG	1 cut(s)	1958
Aor13HI	TCCGGA	1 cut(s)	213
AoxI	GGCC	6 cut(s)	20, 61, 72, 200, 513, 1112
ApaLI	GTGCAC	1 cut(s)	1896
ApeKI	GCWGC	4 cut(s)	82, 85, 153, 1670
ApoI	RAATTY	3 cut(s)	195, 1442, 1568
Asp700I	GAANNNNTTC	2 cut(s)	1212, 1517
AspLEI	GCGC	1 cut(s)	1834
AspS9I	GGNCC	4 cut(s)	513, 1372, 1857, 1865
AsuC2I	CCSGG	1 cut(s)	1861
AsuHPI	GGTGA	5 cut(s)	362, 701, 1265, 1361, 2036
AvaII	GGWCC	3 cut(s)	1372, 1857, 1865
BaeGI	GKGCMC	1 cut(s)	1900
BalI	TGGCCA	1 cut(s)	1114
BamHI	GGATCC	1 cut(s)	1948
BauI	CACGAG	1 cut(s)	508
BbsI	GAAGAC	1 cut(s)	154
Bbv12I	GWGCWC	3 cut(s)	919, 1900, 2093
BbvI	GCAGC	4 cut(s)	72, 94, 165, 1682
BccI	CCATC	6 cut(s)	24, 905, 1028, 1305, 1431, 2027
BciT130I	CCWGG	3 cut(s)	369, 1047, 1347
BciVI	GTATCC	2 cut(s)	778, 955
BclI	TGATCA	3 cut(s)	691, 1726, 1969
BcnI	CCSGG	1 cut(s)	1861
BcoDI	GTCTC	2 cut(s)	717, 1306
BfaI	CTAG	3 cut(s)	587, 1058, 1839
BfmI	CTRYAG	1 cut(s)	1359
BfrI	CTTAAG	1 cut(s)	554
BfuI	GTATCC	2 cut(s)	778, 955
BisI	GCNGC	6 cut(s)	83, 86, 154, 203, 1078, 1671
BlpI	GCTNAGC	1 cut(s)	1800
BlsI	GCNGC	6 cut(s)	84, 87, 155, 204, 1079, 1672
BmcAI	AGTACT	2 cut(s)	998, 1677
Bme1390I	CCNGG	4 cut(s)	369, 1047, 1347, 1861
Bme18I	GGWCC	3 cut(s)	1372, 1857, 1865
BmeRI	GACNNNNNGTC	1 cut(s)	1863
BmgT120I	GGNCC	4 cut(s)	513, 1372, 1857, 1865
BmiI	GGNNCC	6 cut(s)	597, 1373, 1374, 1866, 1950, 2017
BmrFI	CCNGG	4 cut(s)	369, 1047, 1347, 1861
BmsI	GCATC	8 cut(s)	1019, 1135, 1192, 1252, 1393, 1764, 1813, 1843
BpiI	GAAGAC	1 cut(s)	154
BpmI	CTGGAG	1 cut(s)	1517
Bpu1102I	GCTNAGC	1 cut(s)	1800
BpuEI	CTTGAG	1 cut(s)	74
BpuMI	CCSGG	1 cut(s)	1861
BsaAI	YACGTR	1 cut(s)	1223
BsaHI	GRCGYC	1 cut(s)	2028
BsaJI	CCNNGG	4 cut(s)	368, 435, 1081, 1860
BsaWI	WCCGGW	2 cut(s)	213, 1275
Bsc4I	CCNNNNNNNGG	4 cut(s)	70, 1223, 1382, 1958
Bse1I	ACTGG	6 cut(s)	260, 597, 813, 1312, 1852, 2059
Bse3DI	GCAATG	1 cut(s)	876
BseAI	TCCGGA	1 cut(s)	213
BseBI	CCWGG	3 cut(s)	369, 1047, 1347
BseDI	CCNNGG	4 cut(s)	368, 435, 1081, 1860
BseGI	GGATG	8 cut(s)	1183, 1207, 1316, 1442, 1560, 1630, 1834, 2074
BseLI	CCNNNNNNNGG	4 cut(s)	70, 1223, 1382, 1958
BseMI	GCAATG	1 cut(s)	876
BseMII	CTCAG	1 cut(s)	1814
BseNI	ACTGG	6 cut(s)	260, 597, 813, 1312, 1852, 2059
BseRI	GAGGAG	3 cut(s)	40, 623, 909
BseSI	GKGCMC	1 cut(s)	1900
BseX3I	CGGCCG	1 cut(s)	200
BseXI	GCAGC	4 cut(s)	72, 94, 165, 1682
BseYI	CCCAGC	1 cut(s)	1958
Bsh1236I	CGCG	1 cut(s)	2012
Bsh1285I	CGRYCG	1 cut(s)	203
BshFI	GGCC	6 cut(s)	22, 63, 74, 202, 515, 1114
BsiEI	CGRYCG	1 cut(s)	203
BsiHKAI	GWGCWC	3 cut(s)	919, 1900, 2093
BsiSI	CCGG	4 cut(s)	60, 214, 1276, 1860
BslFI	GGGAC	3 cut(s)	767, 1358, 1722
BslI	CCNNNNNNNGG	4 cut(s)	70, 1223, 1382, 1958
BsmAI	GTCTC	2 cut(s)	717, 1306
BsmBI	CGTCTC	1 cut(s)	717
BsmFI	GGGAC	3 cut(s)	767, 1358, 1722
BsnI	GGCC	6 cut(s)	22, 63, 74, 202, 515, 1114
Bsp1286I	GDGCHC	3 cut(s)	919, 1900, 2093
Bsp13I	TCCGGA	1 cut(s)	213
Bsp1407I	TGTACA	1 cut(s)	1332
Bsp143I	GATC	9 cut(s)	312, 457, 691, 1657, 1708, 1726, 1785, 1948, 1969
Bsp1720I	GCTNAGC	1 cut(s)	1800
Bsp19I	CCATGG	1 cut(s)	435
BspACI	CCGC	7 cut(s)	28, 96, 126, 203, 1078, 1792, 2012
BspANI	GGCC	6 cut(s)	22, 63, 74, 202, 515, 1114
BspCNI	CTCAG	1 cut(s)	1813
BspEI	TCCGGA	1 cut(s)	213
BspFNI	CGCG	1 cut(s)	2012
BspLI	GGNNCC	6 cut(s)	597, 1373, 1374, 1866, 1950, 2017
BspPI	GGATC	5 cut(s)	320, 465, 1703, 1943, 1956
BspQI	GCTCTTC	1 cut(s)	1059
BspTI	CTTAAG	1 cut(s)	554
BsrBI	CCGCTC	1 cut(s)	98
BsrDI	GCAATG	1 cut(s)	876
BsrGI	TGTACA	1 cut(s)	1332
BsrI	ACTGG	6 cut(s)	260, 597, 813, 1312, 1852, 2059
BssECI	CCNNGG	4 cut(s)	368, 435, 1081, 1860
BssMI	GATC	9 cut(s)	312, 457, 691, 1657, 1708, 1726, 1785, 1948, 1969
BssNI	GRCGYC	1 cut(s)	2028
BssSI	CACGAG	1 cut(s)	508
BssT1I	CCWWGG	2 cut(s)	435, 1081
Bst2BI	CACGAG	1 cut(s)	508
Bst2UI	CCWGG	3 cut(s)	369, 1047, 1347
Bst4CI	ACNGT	2 cut(s)	350, 1845
Bst6I	CTCTTC	4 cut(s)	948, 1059, 1081, 1638
BstACI	GRCGYC	1 cut(s)	2028
BstAFI	CTTAAG	1 cut(s)	554
BstAPI	GCANNNNNTGC	1 cut(s)	1690
BstAUI	TGTACA	1 cut(s)	1332
BstBAI	YACGTR	1 cut(s)	1223
BstC8I	GCNNGC	6 cut(s)	32, 867, 883, 1071, 1075, 1482
BstDEI	CTNAG	3 cut(s)	1679, 1782, 1800
BstDSI	CCRYGG	1 cut(s)	435
BstENI	CCTNNNNNAGG	1 cut(s)	1380
BstF5I	GGATG	8 cut(s)	1183, 1207, 1316, 1442, 1560, 1630, 1834, 2074
BstFNI	CGCG	1 cut(s)	2012
BstHHI	GCGC	1 cut(s)	1834
BstKTI	GATC	9 cut(s)	315, 460, 694, 1660, 1711, 1729, 1788, 1951, 1972
BstMAI	GTCTC	2 cut(s)	717, 1306
BstMBI	GATC	9 cut(s)	312, 457, 691, 1657, 1708, 1726, 1785, 1948, 1969
BstMCI	CGRYCG	1 cut(s)	203
BstMWI	GCNNNNNNNGC	6 cut(s)	251, 399, 408, 1501, 1670, 1690
BstNI	CCWGG	3 cut(s)	369, 1047, 1347
BstNSI	RCATGY	1 cut(s)	1484
BstSCI	CCNGG	4 cut(s)	367, 1045, 1345, 1859
BstSFI	CTRYAG	1 cut(s)	1359
BstSLI	GKGCMC	1 cut(s)	1900
BstUI	CGCG	1 cut(s)	2012
BstV1I	GCAGC	4 cut(s)	72, 94, 165, 1682
BstV2I	GAAGAC	1 cut(s)	154
BstX2I	RGATCY	2 cut(s)	312, 1948
BstYI	RGATCY	2 cut(s)	312, 1948
BstZI	CGGCCG	1 cut(s)	200
BsuI	GTATCC	2 cut(s)	778, 955
BsuRI	GGCC	6 cut(s)	22, 63, 74, 202, 515, 1114
BtgI	CCRYGG	1 cut(s)	435
BtsCI	GGATG	8 cut(s)	1183, 1207, 1316, 1442, 1560, 1630, 1834, 2074
BtsIMutI	CAGTG	3 cut(s)	113, 1029, 1859
Cac8I	GCNNGC	6 cut(s)	32, 867, 883, 1071, 1075, 1482
CaiI	CAGNNNCTG	1 cut(s)	1958
CfoI	GCGC	1 cut(s)	1834
Cfr13I	GGNCC	4 cut(s)	513, 1372, 1857, 1865
CseI	GACGC	1 cut(s)	2036
CspCI	CAANNNNNGTGG	4 cut(s)	371, 406, 962, 997
CviAII	CATG	7 cut(s)	248, 436, 1162, 1481, 1685, 1817, 2094
DdeI	CTNAG	3 cut(s)	1679, 1782, 1800
DpnI	GATC	9 cut(s)	314, 459, 693, 1659, 1710, 1728, 1787, 1950, 1971
DpnII	GATC	9 cut(s)	312, 457, 691, 1657, 1708, 1726, 1785, 1948, 1969
DraIII	CACNNNGTG	1 cut(s)	605
DriI	GACNNNNNGTC	1 cut(s)	1863
EaeI	YGGCCR	2 cut(s)	200, 1112
EagI	CGGCCG	1 cut(s)	200
Eam1104I	CTCTTC	4 cut(s)	948, 1059, 1081, 1638
Eam1105I	GACNNNNNGTC	1 cut(s)	1863
EarI	CTCTTC	4 cut(s)	948, 1059, 1081, 1638
EclXI	CGGCCG	1 cut(s)	200
Eco130I	CCWWGG	2 cut(s)	435, 1081
Eco32I	GATATC	2 cut(s)	642, 835
Eco47I	GGWCC	3 cut(s)	1372, 1857, 1865
Eco52I	CGGCCG	1 cut(s)	200
Eco57I	CTGAAG	1 cut(s)	1083
EcoNI	CCTNNNNNAGG	1 cut(s)	1380
EcoO109I	RGGNCCY	3 cut(s)	513, 1372, 1865
EcoRII	CCWGG	3 cut(s)	367, 1045, 1345
EcoRV	GATATC	2 cut(s)	642, 835
EcoT14I	CCWWGG	2 cut(s)	435, 1081
EcoT22I	ATGCAT	1 cut(s)	1267
ErhI	CCWWGG	2 cut(s)	435, 1081
Esp3I	CGTCTC	1 cut(s)	717
FaeI	CATG	7 cut(s)	251, 439, 1165, 1484, 1688, 1820, 2097
FalI	AAGNNNNNCTT	6 cut(s)	23, 55, 1121, 1153, 1505, 1537
FaqI	GGGAC	3 cut(s)	767, 1358, 1722
FatI	CATG	7 cut(s)	247, 435, 1161, 1480, 1684, 1816, 2093
FauI	CCCGC	1 cut(s)	2005
FbaI	TGATCA	3 cut(s)	691, 1726, 1969
Fnu4HI	GCNGC	6 cut(s)	83, 86, 154, 203, 1078, 1671
FokI	GGATG	8 cut(s)	1190, 1214, 1323, 1449, 1567, 1637, 1821, 2081
Fsp4HI	GCNGC	6 cut(s)	83, 86, 154, 203, 1078, 1671
FspBI	CTAG	3 cut(s)	587, 1058, 1839
GlaI	GCGC	1 cut(s)	1833
GluI	GCNGC	6 cut(s)	83, 86, 154, 203, 1078, 1671
GsaI	CCCAGC	1 cut(s)	1962
GsuI	CTGGAG	1 cut(s)	1517
HaeIII	GGCC	6 cut(s)	22, 63, 74, 202, 515, 1114
HapII	CCGG	4 cut(s)	60, 214, 1276, 1860
HgaI	GACGC	1 cut(s)	2036
HhaI	GCGC	1 cut(s)	1834
Hin1I	GRCGYC	1 cut(s)	2028
Hin1II	CATG	7 cut(s)	251, 439, 1165, 1484, 1688, 1820, 2097
Hin6I	GCGC	1 cut(s)	1832
HinP1I	GCGC	1 cut(s)	1832
HincII	GTYRAC	1 cut(s)	1356
HindII	GTYRAC	1 cut(s)	1356
HindIII	AAGCTT	2 cut(s)	731, 863
HinfI	GANTC	6 cut(s)	334, 413, 1104, 1279, 1628, 1768
HpaII	CCGG	4 cut(s)	60, 214, 1276, 1860
HphI	GGTGA	5 cut(s)	362, 701, 1265, 1361, 2036
Hpy166II	GTNNAC	7 cut(s)	498, 905, 1356, 1697, 1857, 1898, 1988
Hpy188I	TCNGA	9 cut(s)	171, 418, 448, 631, 696, 720, 1177, 1323, 1773
Hpy188III	TCNNGA	6 cut(s)	214, 316, 752, 1655, 1946, 1952
Hpy8I	GTNNAC	7 cut(s)	498, 905, 1356, 1697, 1857, 1898, 1988
HpyAV	CCTTC	9 cut(s)	49, 132, 151, 313, 842, 952, 1000, 1180, 1987
HpyCH4III	ACNGT	2 cut(s)	350, 1845
HpyCH4IV	ACGT	3 cut(s)	1222, 1531, 1735
HpyF10VI	GCNNNNNNNGC	6 cut(s)	251, 399, 408, 1501, 1670, 1690
HpyF3I	CTNAG	3 cut(s)	1679, 1782, 1800
HpySE526I	ACGT	3 cut(s)	1222, 1531, 1735
Hsp92I	GRCGYC	1 cut(s)	2028
Hsp92II	CATG	7 cut(s)	251, 439, 1165, 1484, 1688, 1820, 2097
HspAI	GCGC	1 cut(s)	1832
KflI	GGGWCCC	1 cut(s)	1372
Kpn2I	TCCGGA	1 cut(s)	213
Ksp22I	TGATCA	3 cut(s)	691, 1726, 1969
Kzo9I	GATC	9 cut(s)	312, 457, 691, 1657, 1708, 1726, 1785, 1948, 1969
LguI	GCTCTTC	1 cut(s)	1059
LmnI	GCTCC	3 cut(s)	103, 922, 1477
Lsp1109I	GCAGC	4 cut(s)	72, 94, 165, 1682
LweI	GCATC	8 cut(s)	1019, 1135, 1192, 1252, 1393, 1764, 1813, 1843
MaeI	CTAG	3 cut(s)	587, 1058, 1839
MaeII	ACGT	3 cut(s)	1222, 1531, 1735
MaeIII	GTNAC	2 cut(s)	1363, 2024
MalI	GATC	9 cut(s)	314, 459, 693, 1659, 1710, 1728, 1787, 1950, 1971
MbiI	CCGCTC	1 cut(s)	98
MboI	GATC	9 cut(s)	312, 457, 691, 1657, 1708, 1726, 1785, 1948, 1969
MboII	GAAGA	8 cut(s)	154, 222, 887, 965, 1076, 1098, 1577, 1625
MflI	RGATCY	2 cut(s)	312, 1948
MhlI	GDGCHC	3 cut(s)	919, 1900, 2093
MlsI	TGGCCA	1 cut(s)	1114
MluNI	TGGCCA	1 cut(s)	1114
MlyI	GAGTC	3 cut(s)	1113, 1273, 1777
MmeI	TCCRAC	1 cut(s)	1414
Mox20I	TGGCCA	1 cut(s)	1114
Mph1103I	ATGCAT	1 cut(s)	1267
MroI	TCCGGA	1 cut(s)	213
MroXI	GAANNNNTTC	2 cut(s)	1212, 1517
MscI	TGGCCA	1 cut(s)	1114
MseI	TTAA	3 cut(s)	555, 1001, 1592
Msp20I	TGGCCA	1 cut(s)	1114
MspA1I	CMGCKG	1 cut(s)	85
MspCI	CTTAAG	1 cut(s)	554
MspI	CCGG	4 cut(s)	60, 214, 1276, 1860
MspR9I	CCNGG	4 cut(s)	369, 1047, 1347, 1861
MvaI	CCWGG	3 cut(s)	369, 1047, 1347
MvnI	CGCG	1 cut(s)	2012
MwoI	GCNNNNNNNGC	6 cut(s)	251, 399, 408, 1501, 1670, 1690
NciI	CCSGG	1 cut(s)	1861
NcoI	CCATGG	1 cut(s)	435
NdeII	GATC	9 cut(s)	312, 457, 691, 1657, 1708, 1726, 1785, 1948, 1969
NlaIII	CATG	7 cut(s)	251, 439, 1165, 1484, 1688, 1820, 2097
NlaIV	GGNNCC	6 cut(s)	597, 1373, 1374, 1866, 1950, 2017
NmuCI	GTSAC	1 cut(s)	2024
NsiI	ATGCAT	1 cut(s)	1267
NspI	RCATGY	1 cut(s)	1484
PaeI	GCATGC	1 cut(s)	1484
PciSI	GCTCTTC	1 cut(s)	1059
PcsI	WCGNNNNNNNCGW	1 cut(s)	1996
PdmI	GAANNNNTTC	2 cut(s)	1212, 1517
PfeI	GAWTC	3 cut(s)	334, 413, 1628
PflMI	CCANNNNNTGG	1 cut(s)	1958
PkrI	GCNGC	6 cut(s)	84, 87, 155, 204, 1079, 1672
PleI	GAGTC	3 cut(s)	1112, 1273, 1776
PpsI	GAGTC	3 cut(s)	1112, 1273, 1776
Ppu21I	YACGTR	1 cut(s)	1223
PpuMI	RGGWCCY	2 cut(s)	1372, 1865
Psp1406I	AACGTT	1 cut(s)	1531
Psp5II	RGGWCCY	2 cut(s)	1372, 1865
Psp6I	CCWGG	3 cut(s)	367, 1045, 1345
PspFI	CCCAGC	1 cut(s)	1958
PspGI	CCWGG	3 cut(s)	367, 1045, 1345
PspN4I	GGNNCC	6 cut(s)	597, 1373, 1374, 1866, 1950, 2017
PspPI	GGNCC	4 cut(s)	513, 1372, 1857, 1865
PspPPI	RGGWCCY	2 cut(s)	1372, 1865
PstNI	CAGNNNCTG	1 cut(s)	1958
PsuI	RGATCY	2 cut(s)	312, 1948
PvuII	CAGCTG	1 cut(s)	85
SapI	GCTCTTC	1 cut(s)	1059
SaqAI	TTAA	3 cut(s)	555, 1001, 1592
SatI	GCNGC	6 cut(s)	83, 86, 154, 203, 1078, 1671
Sau3AI	GATC	9 cut(s)	312, 457, 691, 1657, 1708, 1726, 1785, 1948, 1969
Sau96I	GGNCC	4 cut(s)	513, 1372, 1857, 1865
ScaI	AGTACT	2 cut(s)	998, 1677
SchI	GAGTC	3 cut(s)	1113, 1273, 1777
ScrFI	CCNGG	4 cut(s)	369, 1047, 1347, 1861
SduI	GDGCHC	3 cut(s)	919, 1900, 2093
SfaNI	GCATC	8 cut(s)	1019, 1135, 1192, 1252, 1393, 1764, 1813, 1843
SfcI	CTRYAG	1 cut(s)	1359
SinI	GGWCC	3 cut(s)	1372, 1857, 1865
SmlI	CTYRAG	2 cut(s)	89, 554
SmoI	CTYRAG	2 cut(s)	89, 554
SphI	GCATGC	1 cut(s)	1484
SsiI	CCGC	7 cut(s)	28, 96, 126, 203, 1078, 1792, 2012
SspMI	CTAG	3 cut(s)	587, 1058, 1839
StyD4I	CCNGG	4 cut(s)	367, 1045, 1345, 1859
StyI	CCWWGG	2 cut(s)	435, 1081
TaaI	ACNGT	2 cut(s)	350, 1845
TaiI	ACGT	3 cut(s)	1225, 1534, 1738
TaqI	TCGA	4 cut(s)	179, 759, 1260, 2083
TaqII	GACCGA	1 cut(s)	1766
TatI	WGTACW	9 cut(s)	375, 683, 996, 1021, 1164, 1332, 1447, 1675, 1975
TauI	GCSGC	2 cut(s)	205, 1080
TfiI	GAWTC	3 cut(s)	334, 413, 1628
Tru1I	TTAA	3 cut(s)	555, 1001, 1592
Tru9I	TTAA	3 cut(s)	555, 1001, 1592
TscAI	CASTG	3 cut(s)	120, 1036, 1859
TseFI	GTSAC	1 cut(s)	2024
TseI	GCWGC	4 cut(s)	82, 85, 153, 1670
Tsp45I	GTSAC	1 cut(s)	2024
TspDTI	ATGAA	3 cut(s)	1269, 1894, 1925
TspGWI	ACGGA	4 cut(s)	237, 741, 1567, 2005
TspRI	CASTG	3 cut(s)	120, 1036, 1859
Van91I	CCANNNNNTGG	1 cut(s)	1958
Vha464I	CTTAAG	1 cut(s)	554
VneI	GTGCAC	1 cut(s)	1896
VpaK11BI	GGWCC	3 cut(s)	1372, 1857, 1865
XagI	CCTNNNNNAGG	1 cut(s)	1380
XapI	RAATTY	3 cut(s)	195, 1442, 1568
XceI	RCATGY	1 cut(s)	1484
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1644
XmnI	GAANNNNTTC	2 cut(s)	1212, 1517
XspI	CTAG	3 cut(s)	587, 1058, 1839
ZrmI	AGTACT	2 cut(s)	998, 1677
Zsp2I	ATGCAT	1 cut(s)	1267

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.