Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0001578

Physical Location & Seq

Genomic Coordinates Reverse (-)

81468553 .. 81483925

Gene Structure 15373 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00059480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3669 bp

ATGCGTCTTCTTCTTCTTCATCGACCTCTTCATAAGCTCTCAGCCTTATCTTCAAGCACTTCTCTTCTTATGGGTTCCAAGCAGCTACTAAAGTCCACACCTTTCACAAACCCAACTCCTCCTTCTTCACAAACACACTCCTTTCACTCTCACAGACTCGTTTTCTCTGCATTTCCGCCTAAACCCAGGTCCAAAGCTCGGCCCTTTTGCCTTAGGACTAATGGGTATTCTTCTGTCTCACATGCCAATGTCAAAACCCCAAAACTAGCTGATTTGGAGATAACAGATGAGCAGAGATATGAGGCTTTGACGAAAAAGTTGAAGGAGATTGGCATTGATGATGAAATATGCGAACCAGCGCAGTATGGCCACTTAATTTGTCCAATGTGCAAAGGTGGCGATTCAGAGGAAAAGAGCTTATCCATTTTTATTGAAAAAGATTGTGCCATCGCCGAAAATGTGATTGGAAAGGTTCTTGCATTTTTTTCGGAGCGAGGAATATCAAGGGAAACAGTACAGAGGAATAAAGTAATGCAAAAGCGTTGTTCCATAACAGATCAGATTTCTATTGCATTTACTTATTGGAGGAATGGAAAGCTGATTAGTTGCAAGTATCGGGACATCAACAAGAAGTTCTGGCAGGAGAAGGATACTGAAAGGATTTTTTACGGGCTGGATGATATAAAGGATACAAATGATATCATCATTGTAGAAGGCGAGATGGATAAGCTTGCAATGGAAGAAGCTGGCTATCGCAATTGCGTGAGTGTGCCTGATGGTGCTCCTCCCAAGGCTTCTCCTCCCGATAAGGATGTGCCACCTGAAGAGCAGTGGCTACTGTTAATCCAAATCATCCAATACACCAAATCCCAAAGTTCTGTTTCAGTCAGGTCCCTATATTCTCATGGTAGTTCCAGGGATTATATCGAGAGATGCTGGCGAGTCAGCTGGCCAAAGAAAAATGATCAGGTTGAACACTTCAAAGATGCAAACGAGGTTCTCATGTATTTGGGACCTGCTGTATTGAAGGAAGTAATTGAGAATGCTGAATTATATCCTATACGTGGATTGTTCAGATTTCAAGATTACTTTGATGAAATTAATGCATATTACCATCGGACATATAAACATGATTGCGGTGTCAAAACTGGGTGGAGAAATCTGGATGATTTATACAATGTTGTCCCGGGTGAGTTGACTATAGTAACAGGAGTTCCTAATTCAGGCAAGAGTGAGTGGATTGATGCTCTCTTGTGCAATCTCTATGAAGGATATGGTTGGAAATTTGCACTTTGTTCTATGGAAAACAAGGTTAGGGAGCATGCACGAAAGCTGCTGGAGAAACACATACAGAAGCCTTTCTTTGATGGACGCTATGGTGGTCCTGCTGAACGAATGAGTGTTGAAGAATTTGAGCGCGGGAAACAATGGTTAAATGACACATTTCACCTCATAAGGTGTGAGGATGATTCCCTTCCAAATATCAAATGGGTTCTTGATCTTGCACGAGCAGCAGTACTTAGGCATGGGGTGCGTGGACTTGTAATTGATCCTTACAACGAGCTTGATCATCAACGTCCACCAAACCAGACAGAAACAGAGTATGTAAGTCAGATGCTTACCAATGTCAAACGGTTTGCTCAGCATCATGCTTGTCATGTTTGGGGTCCTCCTAATCTTTATGATATAAGTGGAAGTGCACATTTCATAAACAAATGTGACAATGGAATTGTAATTCATCGTAATCGGGATCCAGAGGCTGGGGCCCTTGATCAAGTACAGGTCTGTGTTCGTAAGGTACGGAATAAAGTCGCAGGAACCATAGGTGACGCCTATTTGTCATATGATAGGTATGGGCAACTGGTCGATATGTGGATATTCCGAAACCAAATGAGGGGAAATAGCAAGTTACGTGATCAAAGCTTGTACAGTGGGAATCTTCTAGCATCTGGGCTGTCTGGTGAGCTTACTTATGGCTTGATCAAACAACCTGTTCTATTATTGCACCAGGATCTCTCAATTTGTATTGAACTCTCAATCAATGGGGATTTGGGGATTTCACCTTTGCTGTGTAGTTGGTGGACAGAGCATAATTGGAGGTGGGAATCCGAGTCCAAGCCAACCACTGTTGAGAAGAGCACTTCTTATAAGGAGGAGAGCAACTATCTCTCTGATCTCAAGGAGTTTGAGAAGAAGGCTTTGGTTGAGCTCAAATCGAAGCTCGAAGAAGCGATTTTGGGCAACAACATTTTCAAGAAAGATCAGCCCAAGAAGGTTGAGGAGAAGGAGAAGGCGGTAGCTGAGGTCGAGGAGAAGAAGGAAGAGAAAACAGAGGCTGCTGTGGAAAAAACAGAGGAAACAGAGGCTGCTGTGGAAAAAACAGAGGAATCAGAGGCTGCTGTGAAAGCAGAGGAAACAGAGCCTGCTGTGAAAGCAGAGGAAACAGAGGCTGTTGTGAAATCAGAGGAAACAGAGGCTGTTGTGAAAACAGATGTTGTGAAAACAGAGTGCGCTGAAAAAACAGAGGAAGGAGAAAAGGCACCAGAATTGGCTGAGAAGAAAGAAGTAGCAGTTCCGGTATGCGAGGAAGAAGCGAAACCCGAATCCAGCGAAGTTGTCGACACAGATATCTCACTCTGGGGTGTGCCTCTGTTCCCTGGAAAAGGATCTGAGGGAACTGATGTGGTGCTTTTGAAGTTCCTGAGGGCTAGGGAGTTCAAGGTCAACGAAGCCTTCGAGATGCTGAAGAAGACTCTGCAGTGGAGGAAGGAATCCAAAATTGATTCAATTTTGGATGAGGAGATCTGCTCTGATCTGAGCTCTGCTGCTTACTTGAACGGCGTGGATCGCGAAGGACACCCTGTTTGCTACAACATCTTCGGGGTTTTTGACAACGAAGAGCTTTACCAGAAGACGTTCGGAAATGAAGAGAAGAGAGGCCAATTCTTGAGGTGGAGGGTTCAATTGATGGAGAAGAGTATTCAGAAGCTTGAATTGAGGCCTGGTGGTGCTACTTCTCTGCTTCAGATCAATGACCTCAAGAACTCGCCTGGGCCAATCAAGAAGGAGCTTAGGGTTGCAACAAAGCAAGCTGTTGGGCTCTTGCAGGACAATTACCCTGAATTGGTTGCCAAAAATATTTTCATCAATGTTCCATTCTGGTACTATGCACTCAATGCTCTGTTGTCTCCATTCTTGACTCAAAGAACCAAGAGCAAGTTTGTGGTGGCTCGCCCCGCTAAGGTCACCGAGACCCTTCTCAAGTACATTCCAGCTGAGGAGATACCAATTCAGTATGGTGGCTTCAAGAGAGAAAATGACACCGAGTTCTTCTCCTCTGAAGATGGTTCTGTTTCTGAGCTAACTCTCAAGGCCGGATCAACCCAAACAATAGAGATTGATGTACCTGAGGTTGATAATAGCACATTGGTTTGGGATGTGAGTGTTTTGGGATGGGAAGTGAATTACAAGGAGGAGTTTGTTCCAAGTGATGAAGGGTCATACACCATTATTGTTCAGAAGAAGAAGAAGAAGATCGGAGCTAATGAAGGGCCTATTCGCAACACTTTCCGAAGCAATGAGCCTGGAAAGGTTGTCCTGACAATCGAGAACACTTCGAGCAAGAAGAAGAGGGTGCTGTACCGGCACAAGTCCAACAAGTGCTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003697 GO:0003824 GO:0003896 GO:0003899 GO:0004003 GO:0004386 GO:0005488 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006269 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009117 GO:0009165 GO:0009259 GO:0009260 GO:0009987 GO:0016043 GO:0016070 GO:0016462 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0032392 GO:0032508 GO:0032774 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0042623 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044281 GO:0046390 GO:0046483 GO:0051276 GO:0055086 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0090407 GO:0097159 GO:0097747 GO:0140097 GO:0140098 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901363 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DnaB_C Toprim_4

Protein Analysis

1222

Amino Acids

139.18

Weight (kDa)

5.85

Isoelectric Point (pI)

42.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRAL_TRIO_N	PF03765	865 - 917	1.2e-08	CRAL/TRIO, N-terminal domain
CRAL_TRIO	PF00650	942 - 1101	6.3e-23	CRAL/TRIO domain
GOLD_PATL1_C	PF25099	1124 - 1210	4.9e-35	Patellin-1-like, C-terminal GOLD domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001578)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30660 AT1G30660 AT1G30660 AT1G30680
fragaria_vesca	FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11220 FvH4_3g11220
malus_domestica	MD05G1265600.v1.1 MD10G1243800.v1.1
prunus_persica	Prupe.4G099700_v2.0.a1 Prupe.4G099700_v2.0.a1 Prupe.4G099700_v2.0.a1 Prupe.4G099700_v2.0.a1 Prupe.4G099900_v2.0.a1 Prupe.4G099900_v2.0.a1
pyrus_communis	pycom05g24970 pycom10g20390
rosa_chinensis	RchiOBHm_Chr5g0018141 RchiOBHm_Chr5g0018171
rosa_laevigata	RLG00000032341 RLG00000032343
rosa_multiflora	Rmu_sc0006594.1_g000001 Rmu_sc0013020.1_g000001 Rmu_sc0013020.1_g000004
rosa_roxburghii	Rroxscaffold_1G00059480 Rroxscaffold_1G00059500
rosa_rugosa	Rorug05G0040700 Rorug05G0040900
rosa_samantha	Rh5AG133500 Rh5AG133800 Rh5BG131900 Rh5BG132300 Rh5CG144200 Rh5CG144500 Rh5DG133900 Rh5DG134200
rosa_wichuraiana	Rw5G011640 Rw5G011690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2148
Acc36I	ACCTGC	1 cut(s)	1024
AccB1I	GGYRCC	1 cut(s)	2539
AccB7I	CCANNNNNTGG	1 cut(s)	1760
AccI	GTMKAC	1 cut(s)	2619
AccII	CGCG	2 cut(s)	1419, 2850
AciI	CCGC	5 cut(s)	176, 1137, 1419, 2294, 3237
AclWI	GGATC	7 cut(s)	1544, 1745, 1758, 2019, 2674, 2853, 3385
AcoI	YGGCCR	2 cut(s)	367, 950
AcsI	RAATTY	2 cut(s)	1283, 1409
AcuI	CTGAAG	4 cut(s)	843, 2765, 3008, 3360
AcyI	GRCGYC	1 cut(s)	1830
AfaI	GTAC	9 cut(s)	516, 1518, 1779, 1800, 1928, 3164, 3266, 3405, 3641
AfiI	CCNNNNNNNGG	8 cut(s)	198, 1064, 1223, 1760, 1894, 2663, 3124, 3241
AjnI	CCWGG	7 cut(s)	185, 914, 2007, 2656, 3001, 3049, 3583
AjuI	GAANNNNNNNTTGG	8 cut(s)	106, 138, 447, 479, 2183, 2215, 3125, 3157
Alw21I	GWGCWC	5 cut(s)	784, 1702, 2141, 2211, 2822
Alw26I	GTCTC	3 cut(s)	241, 3192, 3245
Alw44I	GTGCAC	1 cut(s)	1698
AlwI	GGATC	7 cut(s)	1544, 1745, 1758, 2019, 2674, 2853, 3385
AlwNI	CAGNNNCTG	7 cut(s)	1760, 2336, 2366, 2396, 2423, 2450, 2477
Ama87I	CYCGRG	1 cut(s)	1186
AoxI	GGCC	9 cut(s)	200, 367, 950, 1764, 2938, 2999, 3053, 3372, 3551
ApaI	GGGCCC	1 cut(s)	1768
ApaLI	GTGCAC	1 cut(s)	1698
ApeKI	GCWGC	7 cut(s)	82, 1333, 1511, 2336, 2366, 2396, 2825
ApoI	RAATTY	2 cut(s)	1283, 1409
ArsI	GACNNNNNNTTYG	2 cut(s)	2847, 2879
AseI	ATTAAT	1 cut(s)	1101
Asp700I	GAANNNNTTC	2 cut(s)	1358, 2915
AspLEI	GCGC	3 cut(s)	361, 1419, 2513
AsuC2I	CCSGG	2 cut(s)	1187, 1188
AsuHPI	GGTGA	6 cut(s)	1202, 1439, 1838, 1973, 2052, 3238
AvaI	CYCGRG	1 cut(s)	1186
AvaII	GGWCC	5 cut(s)	189, 891, 1013, 1382, 1667
AxyI	CCTNAGG	3 cut(s)	212, 2702, 3408
BaeGI	GKGCMC	2 cut(s)	1702, 1768
BalI	TGGCCA	2 cut(s)	369, 952
BamHI	GGATCC	1 cut(s)	1750
BanI	GGYRCC	1 cut(s)	2539
BanII	GRGCYC	4 cut(s)	1768, 2211, 2822, 3102
BarI	GAAGNNNNNNTAC	2 cut(s)	3287, 3319
BauI	CACGAG	1 cut(s)	1506
BbsI	GAAGAC	2 cut(s)	2756, 2918
Bbv12I	GWGCWC	5 cut(s)	784, 1702, 2141, 2211, 2822
BbvCI	CCTCAGC	2 cut(s)	2301, 3276
BbvI	GCAGC	7 cut(s)	94, 1320, 1523, 2323, 2353, 2383, 2812
BccI	CCATC	8 cut(s)	455, 715, 770, 1122, 1361, 2962, 3338, 3447
BceAI	ACGGC	1 cut(s)	2854
BcgI	CGANNNNNNTGC	2 cut(s)	468, 502
BciT130I	CCWGG	7 cut(s)	187, 916, 2009, 2658, 3003, 3051, 3585
BciVI	GTATCC	2 cut(s)	643, 682
BclI	TGATCA	5 cut(s)	964, 1567, 1771, 1915, 1980
BcnI	CCSGG	2 cut(s)	1187, 1188
BcoDI	GTCTC	3 cut(s)	241, 3192, 3245
BfaI	CTAG	3 cut(s)	266, 1943, 2709
BfmI	CTRYAG	2 cut(s)	1200, 2756
BfuAI	ACCTGC	1 cut(s)	1024
BfuI	GTATCC	2 cut(s)	643, 682
BglII	AGATCT	1 cut(s)	2802
BisI	GCNGC	7 cut(s)	83, 1334, 1512, 2337, 2367, 2397, 2826
BlpI	GCTNAGC	1 cut(s)	1641
BlsI	GCNGC	7 cut(s)	84, 1335, 1513, 2338, 2368, 2398, 2827
BmcAI	AGTACT	1 cut(s)	1518
Bme1390I	CCNGG	9 cut(s)	187, 916, 1187, 1188, 2009, 2658, 3003, 3051, 3585
Bme18I	GGWCC	5 cut(s)	189, 891, 1013, 1382, 1667
BmeT110I	CYCGRG	1 cut(s)	1186
BmiI	GGNNCC	9 cut(s)	76, 893, 1014, 1668, 1752, 1765, 1766, 1819, 2541
BmrFI	CCNGG	9 cut(s)	187, 916, 1187, 1188, 2009, 2658, 3003, 3051, 3585
BmrI	ACTGGG	1 cut(s)	1158
BmsI	GCATC	7 cut(s)	923, 976, 1234, 1605, 1654, 1955, 2730
BmuI	ACTGGG	1 cut(s)	1158
BpiI	GAAGAC	2 cut(s)	2756, 2918
BplI	GAGNNNNNCTC	4 cut(s)	2792, 2824, 3317, 3349
BpmI	CTGGAG	1 cut(s)	1358
Bpu10I	CCTNAGC	4 cut(s)	2301, 3071, 3240, 3276
Bpu1102I	GCTNAGC	1 cut(s)	1641
BpuEI	CTTGAG	5 cut(s)	2162, 2968, 3023, 3245, 3353
BpuMI	CCSGG	2 cut(s)	1187, 1188
BsaAI	YACGTR	2 cut(s)	1064, 1913
BsaHI	GRCGYC	1 cut(s)	1830
BsaI	GGTCTC	1 cut(s)	3245
BsaJI	CCNNGG	6 cut(s)	185, 789, 915, 1186, 2656, 3050
BsaWI	WCCGGW	1 cut(s)	2575
Bsc4I	CCNNNNNNNGG	8 cut(s)	198, 1064, 1223, 1760, 1894, 2663, 3124, 3241
Bse118I	RCCGGY	1 cut(s)	3642
Bse1I	ACTGG	2 cut(s)	1153, 1866
Bse21I	CCTNAGG	3 cut(s)	212, 2702, 3408
Bse3DI	GCAATG	2 cut(s)	741, 3583
BseBI	CCWGG	7 cut(s)	187, 916, 2009, 2658, 3003, 3051, 3585
BseDI	CCNNGG	6 cut(s)	185, 789, 915, 1186, 2656, 3050
BseGI	GGATG	8 cut(s)	682, 817, 852, 1171, 1471, 2800, 3442, 3458
BseLI	CCNNNNNNNGG	8 cut(s)	198, 1064, 1223, 1760, 1894, 2663, 3124, 3241
BseMI	GCAATG	2 cut(s)	741, 3583
BseNI	ACTGG	2 cut(s)	1153, 1866
BseSI	GKGCMC	2 cut(s)	1702, 1768
BseXI	GCAGC	7 cut(s)	94, 1320, 1523, 2323, 2353, 2383, 2812
BseYI	CCCAGC	1 cut(s)	1760
Bsh1236I	CGCG	2 cut(s)	1419, 2850
BshFI	GGCC	9 cut(s)	202, 369, 952, 1766, 2940, 3001, 3055, 3374, 3553
BshNI	GGYRCC	1 cut(s)	2539
BsiHKAI	GWGCWC	5 cut(s)	784, 1702, 2141, 2211, 2822
BsiHKCI	CYCGRG	1 cut(s)	1186
BsiSI	CCGG	4 cut(s)	1187, 2576, 3375, 3643
BslFI	GGGAC	4 cut(s)	632, 877, 1026, 1169
BslI	CCNNNNNNNGG	8 cut(s)	198, 1064, 1223, 1760, 1894, 2663, 3124, 3241
BsmAI	GTCTC	3 cut(s)	241, 3192, 3245
BsmFI	GGGAC	4 cut(s)	632, 877, 1026, 1169
BsmI	GAATGC	1 cut(s)	1048
BsnI	GGCC	9 cut(s)	202, 369, 952, 1766, 2940, 3001, 3055, 3374, 3553
Bso31I	GGTCTC	1 cut(s)	3245
BsoBI	CYCGRG	1 cut(s)	1186
Bsp120I	GGGCCC	1 cut(s)	1764
Bsp1286I	GDGCHC	7 cut(s)	784, 1702, 1768, 2141, 2211, 2822, 3102
Bsp1407I	TGTACA	1 cut(s)	1926
Bsp1720I	GCTNAGC	1 cut(s)	1641
Bsp68I	TCGCGA	1 cut(s)	2850
BspACI	CCGC	5 cut(s)	176, 1137, 1419, 2294, 3237
BspANI	GGCC	9 cut(s)	202, 369, 952, 1766, 2940, 3001, 3055, 3374, 3553
BspFNI	CGCG	2 cut(s)	1419, 2850
BspLI	GGNNCC	9 cut(s)	76, 893, 1014, 1668, 1752, 1765, 1766, 1819, 2541
BspMAI	CTGCAG	1 cut(s)	2760
BspMI	ACCTGC	1 cut(s)	1024
BspPI	GGATC	7 cut(s)	1544, 1745, 1758, 2019, 2674, 2853, 3385
BspQI	GCTCTTC	3 cut(s)	819, 2129, 2892
BspT107I	GGYRCC	1 cut(s)	2539
BspTNI	GGTCTC	1 cut(s)	3245
BsrDI	GCAATG	2 cut(s)	741, 3583
BsrFI	RCCGGY	1 cut(s)	3642
BsrGI	TGTACA	1 cut(s)	1926
BsrI	ACTGG	2 cut(s)	1153, 1866
BssAI	RCCGGY	1 cut(s)	3642
BssECI	CCNNGG	6 cut(s)	185, 789, 915, 1186, 2656, 3050
BssNI	GRCGYC	1 cut(s)	1830
BssSI	CACGAG	1 cut(s)	1506
BssT1I	CCWWGG	1 cut(s)	789
Bst2BI	CACGAG	1 cut(s)	1506
Bst2UI	CCWGG	7 cut(s)	187, 916, 2009, 2658, 3003, 3051, 3585
Bst4CI	ACNGT	5 cut(s)	514, 840, 1635, 1931, 2128
BstACI	GRCGYC	1 cut(s)	1830
BstAPI	GCANNNNNTGC	2 cut(s)	1531, 3176
BstAUI	TGTACA	1 cut(s)	1926
BstBAI	YACGTR	2 cut(s)	1064, 1913
BstC8I	GCNNGC	8 cut(s)	732, 748, 938, 950, 1323, 2424, 3090, 3232
BstEII	GGTNACC	1 cut(s)	3244
BstENI	CCTNNNNNAGG	2 cut(s)	1221, 2661
BstF5I	GGATG	8 cut(s)	682, 817, 852, 1171, 1471, 2800, 3442, 3458
BstFNI	CGCG	2 cut(s)	1419, 2850
BstHHI	GCGC	3 cut(s)	361, 1419, 2513
BstMAI	GTCTC	3 cut(s)	241, 3192, 3245
BstMWI	GCNNNNNNNGC	9 cut(s)	396, 1511, 1531, 2405, 2432, 2847, 3176, 3236, 3643
BstNI	CCWGG	7 cut(s)	187, 916, 2009, 2658, 3003, 3051, 3585
BstNSI	RCATGY	2 cut(s)	245, 1325
BstPI	GGTNACC	1 cut(s)	3244
BstSCI	CCNGG	9 cut(s)	185, 914, 1185, 1186, 2007, 2656, 3001, 3049, 3583
BstSFI	CTRYAG	2 cut(s)	1200, 2756
BstSLI	GKGCMC	2 cut(s)	1702, 1768
BstUI	CGCG	2 cut(s)	1419, 2850
BstV1I	GCAGC	7 cut(s)	94, 1320, 1523, 2323, 2353, 2383, 2812
BstV2I	GAAGAC	2 cut(s)	2756, 2918
BstX2I	RGATCY	4 cut(s)	1750, 2011, 2666, 2802
BstYI	RGATCY	4 cut(s)	1750, 2011, 2666, 2802
Bsu36I	CCTNAGG	3 cut(s)	212, 2702, 3408
BsuI	GTATCC	2 cut(s)	643, 682
BsuRI	GGCC	9 cut(s)	202, 369, 952, 1766, 2940, 3001, 3055, 3374, 3553
BtgZI	GCGATG	1 cut(s)	433
BtsCI	GGATG	8 cut(s)	682, 817, 852, 1171, 1471, 2800, 3442, 3458
BtsI	GCAGTG	2 cut(s)	836, 2765
BtsIMutI	CAGTG	4 cut(s)	836, 1936, 2124, 2765
BtuMI	TCGCGA	1 cut(s)	2850
BveI	ACCTGC	1 cut(s)	1024
Cac8I	GCNNGC	8 cut(s)	732, 748, 938, 950, 1323, 2424, 3090, 3232
CaiI	CAGNNNCTG	7 cut(s)	1760, 2336, 2366, 2396, 2423, 2450, 2477
CfoI	GCGC	3 cut(s)	361, 1419, 2513
Cfr10I	RCCGGY	1 cut(s)	3642
Cfr9I	CCCGGG	1 cut(s)	1186
CseI	GACGC	2 cut(s)	1380, 1838
Csp6I	GTAC	9 cut(s)	515, 1517, 1778, 1799, 1927, 3163, 3265, 3404, 3640
CspCI	CAANNNNNGTGG	2 cut(s)	359, 394
CviAII	CATG	8 cut(s)	242, 905, 1003, 1130, 1322, 1526, 1649, 1658
CviQI	GTAC	9 cut(s)	515, 1517, 1778, 1799, 1927, 3163, 3265, 3404, 3640
EaeI	YGGCCR	2 cut(s)	367, 950
EciI	GGCGGA	1 cut(s)	165
Ecl136II	GAGCTC	2 cut(s)	2209, 2820
Eco130I	CCWWGG	1 cut(s)	789
Eco147I	AGGCCT	1 cut(s)	3001
Eco24I	GRGCYC	4 cut(s)	1768, 2211, 2822, 3102
Eco31I	GGTCTC	1 cut(s)	3245
Eco32I	GATATC	2 cut(s)	700, 2629
Eco47I	GGWCC	5 cut(s)	189, 891, 1013, 1382, 1667
Eco53kI	GAGCTC	2 cut(s)	2209, 2820
Eco57I	CTGAAG	4 cut(s)	843, 2765, 3008, 3360
Eco81I	CCTNAGG	3 cut(s)	212, 2702, 3408
Eco88I	CYCGRG	1 cut(s)	1186
Eco91I	GGTNACC	1 cut(s)	3244
EcoICRI	GAGCTC	2 cut(s)	2209, 2820
EcoNI	CCTNNNNNAGG	2 cut(s)	1221, 2661
EcoO109I	RGGNCCY	6 cut(s)	891, 1013, 1667, 1764, 1765, 3551
EcoO65I	GGTNACC	1 cut(s)	3244
EcoRII	CCWGG	7 cut(s)	185, 914, 2007, 2656, 3001, 3049, 3583
EcoRV	GATATC	2 cut(s)	700, 2629
EcoT14I	CCWWGG	1 cut(s)	789
EcoT22I	ATGCAT	1 cut(s)	1108
EcoT38I	GRGCYC	4 cut(s)	1768, 2211, 2822, 3102
ErhI	CCWWGG	1 cut(s)	789
FaeI	CATG	8 cut(s)	245, 908, 1006, 1133, 1325, 1529, 1652, 1661
FalI	AAGNNNNNCTT	2 cut(s)	1346, 1378
FaqI	GGGAC	4 cut(s)	632, 877, 1026, 1169
FatI	CATG	8 cut(s)	241, 904, 1002, 1129, 1321, 1525, 1648, 1657
FauI	CCCGC	2 cut(s)	1412, 3244
FauNDI	CATATG	1 cut(s)	1843
FbaI	TGATCA	5 cut(s)	964, 1567, 1771, 1915, 1980
FblI	GTMKAC	1 cut(s)	2619
Fnu4HI	GCNGC	7 cut(s)	83, 1334, 1512, 2337, 2367, 2397, 2826
FokI	GGATG	8 cut(s)	689, 824, 839, 1178, 1478, 2807, 3449, 3465
FriOI	GRGCYC	4 cut(s)	1768, 2211, 2822, 3102
Fsp4HI	GCNGC	7 cut(s)	83, 1334, 1512, 2337, 2367, 2397, 2826
FspBI	CTAG	3 cut(s)	266, 1943, 2709
GlaI	GCGC	3 cut(s)	360, 1418, 2512
GluI	GCNGC	7 cut(s)	83, 1334, 1512, 2337, 2367, 2397, 2826
GsaI	CCCAGC	1 cut(s)	1764
GsuI	CTGGAG	1 cut(s)	1358
HaeIII	GGCC	9 cut(s)	202, 369, 952, 1766, 2940, 3001, 3055, 3374, 3553
HapII	CCGG	4 cut(s)	1187, 2576, 3375, 3643
HgaI	GACGC	2 cut(s)	1380, 1838
HhaI	GCGC	3 cut(s)	361, 1419, 2513
Hin1I	GRCGYC	1 cut(s)	1830
Hin1II	CATG	8 cut(s)	245, 908, 1006, 1133, 1325, 1529, 1652, 1661
Hin6I	GCGC	3 cut(s)	359, 1417, 2511
HinP1I	GCGC	3 cut(s)	359, 1417, 2511
HincII	GTYRAC	3 cut(s)	1197, 2620, 2725
HindII	GTYRAC	3 cut(s)	1197, 2620, 2725
HindIII	AAGCTT	3 cut(s)	728, 1921, 2987
HpaII	CCGG	4 cut(s)	1187, 2576, 3375, 3643
HphI	GGTGA	6 cut(s)	1202, 1439, 1838, 1973, 2052, 3238
Hpy166II	GTNNAC	8 cut(s)	96, 1197, 1538, 1580, 1700, 2082, 2620, 2725
Hpy8I	GTNNAC	8 cut(s)	96, 1197, 1538, 1580, 1700, 2082, 2620, 2725
HpyCH4III	ACNGT	5 cut(s)	514, 840, 1635, 1931, 2128
HpyCH4IV	ACGT	4 cut(s)	1063, 1576, 1912, 2915
HpyF10VI	GCNNNNNNNGC	9 cut(s)	396, 1511, 1531, 2405, 2432, 2847, 3176, 3236, 3643
HpySE526I	ACGT	4 cut(s)	1063, 1576, 1912, 2915
Hsp92I	GRCGYC	1 cut(s)	1830
Hsp92II	CATG	8 cut(s)	245, 908, 1006, 1133, 1325, 1529, 1652, 1661
HspAI	GCGC	3 cut(s)	359, 1417, 2511
Ksp22I	TGATCA	5 cut(s)	964, 1567, 1771, 1915, 1980
LguI	GCTCTTC	3 cut(s)	819, 2129, 2892
LmnI	GCTCC	5 cut(s)	490, 787, 1318, 3067, 3539
Lsp1109I	GCAGC	7 cut(s)	94, 1320, 1523, 2323, 2353, 2383, 2812
LweI	GCATC	7 cut(s)	923, 976, 1234, 1605, 1654, 1955, 2730
MaeI	CTAG	3 cut(s)	266, 1943, 2709
MaeII	ACGT	4 cut(s)	1063, 1576, 1912, 2915
MaeIII	GTNAC	5 cut(s)	1204, 1718, 1826, 1908, 3244
MfeI	CAATTG	2 cut(s)	757, 2963
MflI	RGATCY	4 cut(s)	1750, 2011, 2666, 2802
MhlI	GDGCHC	7 cut(s)	784, 1702, 1768, 2141, 2211, 2822, 3102
MlsI	TGGCCA	2 cut(s)	369, 952
MluNI	TGGCCA	2 cut(s)	369, 952
MlyI	GAGTC	5 cut(s)	150, 951, 2120, 2746, 3193
MmeI	TCCRAC	1 cut(s)	1259
Mox20I	TGGCCA	2 cut(s)	369, 952
Mph1103I	ATGCAT	1 cut(s)	1108
MroXI	GAANNNNTTC	2 cut(s)	1358, 2915
MscI	TGGCCA	2 cut(s)	369, 952
MseI	TTAA	4 cut(s)	374, 842, 1101, 1433
MslI	CAYNNNNRTG	1 cut(s)	246
Msp20I	TGGCCA	2 cut(s)	369, 952
MspA1I	CMGCKG	2 cut(s)	948, 3275
MspI	CCGG	4 cut(s)	1187, 2576, 3375, 3643
MspR9I	CCNGG	9 cut(s)	187, 916, 1187, 1188, 2009, 2658, 3003, 3051, 3585
MunI	CAATTG	2 cut(s)	757, 2963
Mva1269I	GAATGC	1 cut(s)	1048
MvaI	CCWGG	7 cut(s)	187, 916, 2009, 2658, 3003, 3051, 3585
MvnI	CGCG	2 cut(s)	1419, 2850
MwoI	GCNNNNNNNGC	9 cut(s)	396, 1511, 1531, 2405, 2432, 2847, 3176, 3236, 3643
NciI	CCSGG	2 cut(s)	1187, 1188
NdeI	CATATG	1 cut(s)	1843
NlaIII	CATG	8 cut(s)	245, 908, 1006, 1133, 1325, 1529, 1652, 1661
NlaIV	GGNNCC	9 cut(s)	76, 893, 1014, 1668, 1752, 1765, 1766, 1819, 2541
NmeAIII	GCCGAG	1 cut(s)	178
NmuCI	GTSAC	3 cut(s)	1718, 1826, 3244
NruI	TCGCGA	1 cut(s)	2850
NsiI	ATGCAT	1 cut(s)	1108
NspI	RCATGY	2 cut(s)	245, 1325
PaeI	GCATGC	1 cut(s)	1325
PceI	AGGCCT	1 cut(s)	3001
PciSI	GCTCTTC	3 cut(s)	819, 2129, 2892
PcsI	WCGNNNNNNNCGW	1 cut(s)	2733
PctI	GAATGC	1 cut(s)	1048
PdmI	GAANNNNTTC	2 cut(s)	1358, 2915
PfeI	GAWTC	8 cut(s)	401, 1469, 1936, 2105, 2387, 2603, 2771, 2783
PflMI	CCANNNNNTGG	1 cut(s)	1760
PkrI	GCNGC	7 cut(s)	84, 1335, 1513, 2338, 2368, 2398, 2827
PleI	GAGTC	5 cut(s)	150, 950, 2119, 2746, 3193
PpsI	GAGTC	5 cut(s)	150, 950, 2119, 2746, 3193
Ppu21I	YACGTR	2 cut(s)	1064, 1913
PpuMI	RGGWCCY	3 cut(s)	891, 1013, 1667
PshBI	ATTAAT	1 cut(s)	1101
PsiI	TTATAA	1 cut(s)	2148
Psp124BI	GAGCTC	2 cut(s)	2211, 2822
Psp5II	RGGWCCY	3 cut(s)	891, 1013, 1667
Psp6I	CCWGG	7 cut(s)	185, 914, 2007, 2656, 3001, 3049, 3583
PspEI	GGTNACC	1 cut(s)	3244
PspFI	CCCAGC	1 cut(s)	1760
PspGI	CCWGG	7 cut(s)	185, 914, 2007, 2656, 3001, 3049, 3583
PspN4I	GGNNCC	9 cut(s)	76, 893, 1014, 1668, 1752, 1765, 1766, 1819, 2541
PspOMI	GGGCCC	1 cut(s)	1764
PspPPI	RGGWCCY	3 cut(s)	891, 1013, 1667
PsrI	GAACNNNNNNTAC	2 cut(s)	1197, 1229
PstI	CTGCAG	1 cut(s)	2760
PstNI	CAGNNNCTG	7 cut(s)	1760, 2336, 2366, 2396, 2423, 2450, 2477
PsuI	RGATCY	4 cut(s)	1750, 2011, 2666, 2802
PvuII	CAGCTG	2 cut(s)	948, 3275
RruI	TCGCGA	1 cut(s)	2850
RsaI	GTAC	9 cut(s)	516, 1518, 1779, 1800, 1928, 3164, 3266, 3405, 3641
RsaNI	GTAC	9 cut(s)	515, 1517, 1778, 1799, 1927, 3163, 3265, 3404, 3640
RseI	CAYNNNNRTG	1 cut(s)	246
SacI	GAGCTC	2 cut(s)	2211, 2822
SalI	GTCGAC	1 cut(s)	2618
SapI	GCTCTTC	3 cut(s)	819, 2129, 2892
SaqAI	TTAA	4 cut(s)	374, 842, 1101, 1433
SatI	GCNGC	7 cut(s)	83, 1334, 1512, 2337, 2367, 2397, 2826
ScaI	AGTACT	1 cut(s)	1518
SchI	GAGTC	5 cut(s)	150, 951, 2120, 2746, 3193
ScrFI	CCNGG	9 cut(s)	187, 916, 1187, 1188, 2009, 2658, 3003, 3051, 3585
SduI	GDGCHC	7 cut(s)	784, 1702, 1768, 2141, 2211, 2822, 3102
SfaNI	GCATC	7 cut(s)	923, 976, 1234, 1605, 1654, 1955, 2730
SfcI	CTRYAG	2 cut(s)	1200, 2756
SinI	GGWCC	5 cut(s)	189, 891, 1013, 1382, 1667
SmaI	CCCGGG	1 cut(s)	1188
SmiMI	CAYNNNNRTG	1 cut(s)	246
SmlI	CTYRAG	5 cut(s)	2177, 2947, 3038, 3260, 3368
SmoI	CTYRAG	5 cut(s)	2177, 2947, 3038, 3260, 3368
SphI	GCATGC	1 cut(s)	1325
SseBI	AGGCCT	1 cut(s)	3001
SsiI	CCGC	5 cut(s)	176, 1137, 1419, 2294, 3237
SspI	AATATT	1 cut(s)	3139
SspMI	CTAG	3 cut(s)	266, 1943, 2709
SstI	GAGCTC	2 cut(s)	2211, 2822
StuI	AGGCCT	1 cut(s)	3001
StyD4I	CCNGG	9 cut(s)	185, 914, 1185, 1186, 2007, 2656, 3001, 3049, 3583
StyI	CCWWGG	1 cut(s)	789
TaaI	ACNGT	5 cut(s)	514, 840, 1635, 1931, 2128
TaiI	ACGT	4 cut(s)	1066, 1579, 1915, 2918
TatI	WGTACW	5 cut(s)	514, 1516, 1777, 1926, 3264
TfiI	GAWTC	8 cut(s)	401, 1469, 1936, 2105, 2387, 2603, 2771, 2783
Tru1I	TTAA	4 cut(s)	374, 842, 1101, 1433
Tru9I	TTAA	4 cut(s)	374, 842, 1101, 1433
TscAI	CASTG	4 cut(s)	836, 1936, 2131, 2765
TseFI	GTSAC	3 cut(s)	1718, 1826, 3244
TseI	GCWGC	7 cut(s)	82, 1333, 1511, 2336, 2366, 2396, 2825
Tsp45I	GTSAC	3 cut(s)	1718, 1826, 3244
TspGWI	ACGGA	1 cut(s)	1816
TspMI	CCCGGG	1 cut(s)	1186
TspRI	CASTG	4 cut(s)	836, 1936, 2131, 2765
Van91I	CCANNNNNTGG	1 cut(s)	1760
VneI	GTGCAC	1 cut(s)	1698
VpaK11BI	GGWCC	5 cut(s)	189, 891, 1013, 1382, 1667
VspI	ATTAAT	1 cut(s)	1101
XagI	CCTNNNNNAGG	2 cut(s)	1221, 2661
XapI	RAATTY	2 cut(s)	1283, 1409
XceI	RCATGY	2 cut(s)	245, 1325
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1485
XmaI	CCCGGG	1 cut(s)	1186
XmiI	GTMKAC	1 cut(s)	2619
XmnI	GAANNNNTTC	2 cut(s)	1358, 2915
XspI	CTAG	3 cut(s)	266, 1943, 2709
ZrmI	AGTACT	1 cut(s)	1518
Zsp2I	ATGCAT	1 cut(s)	1108

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.