Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0001578

Physical Location & Seq

Genomic Coordinates Forward (+)

13620625 .. 13633147

Gene Structure 12523 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG133800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2109 bp

ATGCGTCTTCTTCTTCTTCATCGACCTCTTCATAAGCTTTCAGCCTTATCTTCAAGCACTGCTCTTCTCATGGGTTCCAAGCAGCTACTAAAGTCCACACCTTTCACAAACCCAACTCCTCCTTCTTCACAAACACACTCCTTTCACTCTCACAGACTCGTTTTCTCTGCATTTCCGCCTAAACCCAGGTCCAAAGCTCGTCCCTTTTGCCTCAGGACTAATGGGTATTCTTATGTCTCACATGCCAATGTCAAAACCCCAAAACTAGCTGATTTGGAGATAACAGATGAGCAGAGATATGAGGCTTTGACGAAAAAGTTGAAGGAGATTGGCATTGATGATGAAATATGTGAACCGGCGCAGTACGGCCACTTAATTTGTCCAATGTGCAAAGGTGGTGATTCAGAGGAAAAGAGCTTATCCATTTTTATTGAAAAAGATTGGAGTTATGCTACTTGGCAGTGCCATCGCCGAAAATGTGATTGGAAAGGTAGAACAATGGCCTTTGCAGGAAGCAAGTCTTCTTATAAAAAATCAAAGAATAGCACGACAGATAAGACAAAAAGGGAAATCGCAGTGGAAAGTTTAGGACTGGAACCACTTTGTGAGGAGGTTCTTGCATTTTTTTCGGAGCGAGGAATATCAAGGGAAACAGTACAGAGGAATAAAGTAATGCAAAAGCGTTGTTCCATAACAGATCAGATTTCTATTGCATTTACTTATTGGAGGAATGGAAAGCTGATTAGTTGCAAGTATCGGGACATCAACAAGAAGTTCTGGCAGGAGAAGGATACTGAAAGGATTTTTTACGGGCTGGATGATATAAAGGATACAAATGATATTATCATTGTAGAAGGCGAGATGGATAAGCTTGCAATGGAAGAAGCTGGCTATCGTAATTGCGTGAGTGTGCCTGATGGTGCTCCTCCCAAGGCTTCTCCTCCCGATAAGGATGTGCCACCTGAAGAGCAGGATACAAAGTATCAATTTCTGTGGAACTGCAAAGAGTACTTAAAAAAGGAATCACGCATTATACTTGCCACTGATGGAGATGGGCCTGGTCAAGCCCTAGCTGAAGAACTTGCCCGCCGCCTTGGAAGAGAAAGATGCTGGCGAGTCAGCTGGCCAAAGAAAAATGATCAGGTTGAACACTTCAAAGATGCAAACGAGGTTCTCATGTATTTGGGACCTGCTGTATTGAAGGAAGTAATTGAGAATGCTGAATTATATCCTATACGTGGATTGTTCAGATTTCAAGATTACTTTGATGAAATTAATGCATATTACCATCGGACATATAAACATGATTGCGGTGTCAAAACTGGGTGGAGAGATCTGGATGATTTATACAATGTTGTCCCGGGTGAGTTGACTATAGTAACAGGAGTTCCTAATTCAGGCAAGAGTGAGTGGATTGATGCTCTCTTGTGCAATCTCTATGAAGGATATGGTTGGAAATTTGCACTTTGTTCCATGGAAAACAAGGTTAGGGAGCATGCACGAAAGCTGCTGGAGAAACACATACAGAAGCCTTTCTTTGATGGACGCTATGGTGGTCCTGCTGAACGAATGAGTGTTGAAGAATTTGAGCGCGGGAAACAATGGTTAAATGACACATTTCACCTCATAAGGTGTGAGGATGATTCCCTTCCAAATATCAAATGGGTTCTTGATCTTGCACGAGCAGCAGTACTTAGGCATGGGGTGCGTGGACTTGTAATTGATCCTTACAACGAGCTTGATCATCAACGTCCACCAAACCAGACAGAAACAGAGTATGTAAGTCAGATGCTTACCAATGTCAAACGGTTTGCTCAGCATCATGCTTGTCATGTTTGGTTTGTAGCACATCCCAGACAGTTGCACCATTGGGTAGGGGGTCCTCCTAATCTTTATGATATAAGTGGAAGTGCACATTTCATAAACAAATGTGACAATGGAATTGTAATTCATCGAAATCGGGATCCAGAGGCTGGGGCCCTTGATCAAGTACAGGTGAGTATACCTAAATTTTCTACGGGCAATTACACATACACAATTCAATTCTTCCTCTCTGTTCCCATAAAATCCCACCCCTGGCATTTAACAAACCATGGCCATCACTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003697 GO:0003824 GO:0003896 GO:0003899 GO:0004003 GO:0004386 GO:0005488 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006269 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009117 GO:0009165 GO:0009259 GO:0009260 GO:0009987 GO:0016043 GO:0016070 GO:0016462 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0032392 GO:0032508 GO:0032774 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0042623 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044281 GO:0046390 GO:0046483 GO:0051276 GO:0055086 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0090407 GO:0097159 GO:0097747 GO:0140097 GO:0140098 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901363 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DnaB_C Toprim_4

Protein Analysis

702

Amino Acids

80.92

Weight (kDa)

8.26

Isoelectric Point (pI)

34.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Toprim_4	PF13662	280 - 366	8e-13	Toprim domain
Toprim	PF01751	281 - 363	2e-07	Toprim domain
DnaB_C	PF03796	438 - 674	5.9e-07	DnaB-like helicase C terminal domain
AAA_25	PF13481	452 - 619	4e-07	AAA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001578)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30660 AT1G30660 AT1G30660 AT1G30680
fragaria_vesca	FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11200 FvH4_3g11220 FvH4_3g11220
malus_domestica	MD05G1265600.v1.1 MD10G1243800.v1.1
prunus_persica	Prupe.4G099700_v2.0.a1 Prupe.4G099700_v2.0.a1 Prupe.4G099700_v2.0.a1 Prupe.4G099700_v2.0.a1 Prupe.4G099900_v2.0.a1 Prupe.4G099900_v2.0.a1
pyrus_communis	pycom05g24970 pycom10g20390
rosa_chinensis	RchiOBHm_Chr5g0018141 RchiOBHm_Chr5g0018171
rosa_laevigata	RLG00000032341 RLG00000032343
rosa_multiflora	Rmu_sc0006594.1_g000001 Rmu_sc0013020.1_g000001 Rmu_sc0013020.1_g000004
rosa_roxburghii	Rroxscaffold_1G00059480 Rroxscaffold_1G00059500
rosa_rugosa	Rorug05G0040700 Rorug05G0040900
rosa_samantha	Rh5AG133500 Rh5AG133800 Rh5BG131900 Rh5BG132300 Rh5CG144200 Rh5CG144500 Rh5DG133900 Rh5DG134200
rosa_wichuraiana	Rw5G011640 Rw5G011690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	528
Acc36I	ACCTGC	1 cut(s)	1198
AccB7I	CCANNNNNTGG	1 cut(s)	1973
AccI	GTMKAC	1 cut(s)	2002
AccII	CGCG	1 cut(s)	1593
AciI	CCGC	5 cut(s)	176, 1087, 1090, 1311, 1593
AclWI	GGATC	3 cut(s)	1718, 1958, 1971
AcoI	YGGCCR	3 cut(s)	367, 1124, 2095
AcsI	RAATTY	3 cut(s)	1457, 1583, 2009
AcuI	CTGAAG	2 cut(s)	984, 1095
AdeI	CACNNNGTG	1 cut(s)	605
AfaI	GTAC	5 cut(s)	365, 657, 1010, 1692, 1992
AfiI	CCNNNNNNNGG	4 cut(s)	1238, 1397, 1973, 2076
AgsI	TTSAA	9 cut(s)	54, 322, 434, 1148, 1156, 1201, 1256, 1580, 2042
AjnI	CCWGG	3 cut(s)	185, 1057, 2075
AjuI	GAANNNNNNNTTGG	4 cut(s)	106, 138, 466, 498
Alw21I	GWGCWC	2 cut(s)	925, 1915
Alw26I	GTCTC	1 cut(s)	241
Alw44I	GTGCAC	1 cut(s)	1911
AlwI	GGATC	3 cut(s)	1718, 1958, 1971
AlwNI	CAGNNNCTG	1 cut(s)	1973
Ama87I	CYCGRG	1 cut(s)	1360
AoxI	GGCC	6 cut(s)	367, 501, 1055, 1124, 1977, 2095
ApaI	GGGCCC	1 cut(s)	1981
ApaLI	GTGCAC	1 cut(s)	1911
ApeKI	GCWGC	3 cut(s)	82, 1507, 1685
ApoI	RAATTY	3 cut(s)	1457, 1583, 2009
AseI	ATTAAT	1 cut(s)	1275
Asp700I	GAANNNNTTC	1 cut(s)	1532
AspLEI	GCGC	2 cut(s)	361, 1593
AspS9I	GGNCC	7 cut(s)	189, 1055, 1187, 1556, 1880, 1977, 1978
AsuC2I	CCSGG	2 cut(s)	1361, 1362
AsuHPI	GGTGA	4 cut(s)	410, 1376, 1613, 2008
AvaI	CYCGRG	1 cut(s)	1360
AvaII	GGWCC	4 cut(s)	189, 1187, 1556, 1880
AxyI	CCTNAGG	1 cut(s)	212
BaeGI	GKGCMC	2 cut(s)	1915, 1981
BalI	TGGCCA	2 cut(s)	1126, 2097
BamHI	GGATCC	1 cut(s)	1963
BanII	GRGCYC	1 cut(s)	1981
BauI	CACGAG	1 cut(s)	1680
BbsI	GAAGAC	1 cut(s)	513
Bbv12I	GWGCWC	2 cut(s)	925, 1915
BbvI	GCAGC	3 cut(s)	94, 1494, 1697
BccI	CCATC	8 cut(s)	474, 856, 911, 1040, 1046, 1296, 1535, 2106
BceAI	ACGGC	1 cut(s)	382
BcgI	CGANNNNNNTGC	4 cut(s)	449, 483, 609, 643
BciT130I	CCWGG	3 cut(s)	187, 1059, 2077
BciVI	GTATCC	3 cut(s)	784, 823, 967
BclI	TGATCA	3 cut(s)	1138, 1741, 1984
BcnI	CCSGG	2 cut(s)	1361, 1362
BcoDI	GTCTC	1 cut(s)	241
BfaI	CTAG	2 cut(s)	266, 1070
BfmI	CTRYAG	1 cut(s)	1374
BfuAI	ACCTGC	1 cut(s)	1198
BfuI	GTATCC	3 cut(s)	784, 823, 967
BglII	AGATCT	1 cut(s)	1333
BisI	GCNGC	4 cut(s)	83, 1090, 1508, 1686
BlpI	GCTNAGC	1 cut(s)	1815
BlsI	GCNGC	4 cut(s)	84, 1091, 1509, 1687
BmcAI	AGTACT	2 cut(s)	1010, 1692
Bme1390I	CCNGG	5 cut(s)	187, 1059, 1361, 1362, 2077
Bme18I	GGWCC	4 cut(s)	189, 1187, 1556, 1880
BmeT110I	CYCGRG	1 cut(s)	1360
BmgT120I	GGNCC	7 cut(s)	189, 1055, 1187, 1556, 1880, 1977, 1978
BmiI	GGNNCC	7 cut(s)	76, 597, 1188, 1881, 1965, 1978, 1979
BmrFI	CCNGG	5 cut(s)	187, 1059, 1361, 1362, 2077
BmrI	ACTGGG	1 cut(s)	1332
BmsI	GCATC	5 cut(s)	1097, 1150, 1408, 1779, 1828
BmuI	ACTGGG	1 cut(s)	1332
BpiI	GAAGAC	1 cut(s)	513
BpmI	CTGGAG	1 cut(s)	1532
Bpu1102I	GCTNAGC	1 cut(s)	1815
BpuMI	CCSGG	2 cut(s)	1361, 1362
BsaAI	YACGTR	1 cut(s)	1238
BsaJI	CCNNGG	7 cut(s)	185, 930, 1093, 1360, 1473, 2075, 2092
Bsc4I	CCNNNNNNNGG	4 cut(s)	1238, 1397, 1973, 2076
Bse118I	RCCGGY	1 cut(s)	355
Bse1I	ACTGG	2 cut(s)	597, 1327
Bse21I	CCTNAGG	1 cut(s)	212
Bse3DI	GCAATG	1 cut(s)	882
BseBI	CCWGG	3 cut(s)	187, 1059, 2077
BseDI	CCNNGG	7 cut(s)	185, 930, 1093, 1360, 1473, 2075, 2092
BseGI	GGATG	5 cut(s)	823, 958, 1345, 1645, 1849
BseLI	CCNNNNNNNGG	4 cut(s)	1238, 1397, 1973, 2076
BseMI	GCAATG	1 cut(s)	882
BseMII	CTCAG	2 cut(s)	226, 1829
BseNI	ACTGG	2 cut(s)	597, 1327
BseRI	GAGGAG	4 cut(s)	108, 623, 915, 930
BseSI	GKGCMC	2 cut(s)	1915, 1981
BseXI	GCAGC	3 cut(s)	94, 1494, 1697
BseYI	CCCAGC	1 cut(s)	1973
Bsh1236I	CGCG	1 cut(s)	1593
BshFI	GGCC	6 cut(s)	369, 503, 1057, 1126, 1979, 2097
BsiHKAI	GWGCWC	2 cut(s)	925, 1915
BsiHKCI	CYCGRG	1 cut(s)	1360
BsiSI	CCGG	2 cut(s)	356, 1361
BslFI	GGGAC	4 cut(s)	186, 773, 1200, 1343
BslI	CCNNNNNNNGG	4 cut(s)	1238, 1397, 1973, 2076
BsmAI	GTCTC	1 cut(s)	241
BsmFI	GGGAC	4 cut(s)	186, 773, 1200, 1343
BsmI	GAATGC	1 cut(s)	1222
BsnI	GGCC	6 cut(s)	369, 503, 1057, 1126, 1979, 2097
BsoBI	CYCGRG	1 cut(s)	1360
Bsp120I	GGGCCC	1 cut(s)	1977
Bsp1286I	GDGCHC	3 cut(s)	925, 1915, 1981
Bsp143I	GATC	8 cut(s)	697, 1138, 1333, 1672, 1723, 1741, 1963, 1984
Bsp1720I	GCTNAGC	1 cut(s)	1815
Bsp19I	CCATGG	2 cut(s)	1473, 2092
BspACI	CCGC	5 cut(s)	176, 1087, 1090, 1311, 1593
BspANI	GGCC	6 cut(s)	369, 503, 1057, 1126, 1979, 2097
BspCNI	CTCAG	2 cut(s)	225, 1828
BspFNI	CGCG	1 cut(s)	1593
BspLI	GGNNCC	7 cut(s)	76, 597, 1188, 1881, 1965, 1978, 1979
BspMI	ACCTGC	1 cut(s)	1198
BspPI	GGATC	3 cut(s)	1718, 1958, 1971
BspQI	GCTCTTC	2 cut(s)	69, 960
BsrDI	GCAATG	1 cut(s)	882
BsrFI	RCCGGY	1 cut(s)	355
BsrI	ACTGG	2 cut(s)	597, 1327
BssAI	RCCGGY	1 cut(s)	355
BssECI	CCNNGG	7 cut(s)	185, 930, 1093, 1360, 1473, 2075, 2092
BssMI	GATC	8 cut(s)	697, 1138, 1333, 1672, 1723, 1741, 1963, 1984
BssNAI	GTATAC	1 cut(s)	2003
BssSI	CACGAG	1 cut(s)	1680
BssT1I	CCWWGG	4 cut(s)	930, 1093, 1473, 2092
Bst1107I	GTATAC	1 cut(s)	2003
Bst2BI	CACGAG	1 cut(s)	1680
Bst2UI	CCWGG	3 cut(s)	187, 1059, 2077
Bst4CI	ACNGT	3 cut(s)	655, 1809, 1860
Bst6I	CTCTTC	4 cut(s)	33, 69, 960, 1093
BstAPI	GCANNNNNTGC	1 cut(s)	1705
BstBAI	YACGTR	1 cut(s)	1238
BstC8I	GCNNGC	6 cut(s)	873, 889, 1087, 1112, 1124, 1497
BstDEI	CTNAG	3 cut(s)	212, 1694, 1815
BstDSI	CCRYGG	2 cut(s)	1473, 2092
BstENI	CCTNNNNNAGG	1 cut(s)	1395
BstF5I	GGATG	5 cut(s)	823, 958, 1345, 1645, 1849
BstFNI	CGCG	1 cut(s)	1593
BstHHI	GCGC	2 cut(s)	361, 1593
BstKTI	GATC	8 cut(s)	700, 1141, 1336, 1675, 1726, 1744, 1966, 1987
BstMAI	GTCTC	1 cut(s)	241
BstMBI	GATC	8 cut(s)	697, 1138, 1333, 1672, 1723, 1741, 1963, 1984
BstMWI	GCNNNNNNNGC	2 cut(s)	1685, 1705
BstNI	CCWGG	3 cut(s)	187, 1059, 2077
BstNSI	RCATGY	2 cut(s)	245, 1499
BstSCI	CCNGG	5 cut(s)	185, 1057, 1359, 1360, 2075
BstSFI	CTRYAG	1 cut(s)	1374
BstSLI	GKGCMC	2 cut(s)	1915, 1981
BstUI	CGCG	1 cut(s)	1593
BstV1I	GCAGC	3 cut(s)	94, 1494, 1697
BstV2I	GAAGAC	1 cut(s)	513
BstX2I	RGATCY	2 cut(s)	1333, 1963
BstYI	RGATCY	2 cut(s)	1333, 1963
BstZ17I	GTATAC	1 cut(s)	2003
Bsu36I	CCTNAGG	1 cut(s)	212
BsuI	GTATCC	3 cut(s)	784, 823, 967
BsuRI	GGCC	6 cut(s)	369, 503, 1057, 1126, 1979, 2097
BtgI	CCRYGG	2 cut(s)	1473, 2092
BtgZI	GCGATG	1 cut(s)	452
BtsCI	GGATG	5 cut(s)	823, 958, 1345, 1645, 1849
BtsI	GCAGTG	3 cut(s)	57, 467, 582
BtsIMutI	CAGTG	4 cut(s)	57, 467, 582, 1041
BveI	ACCTGC	1 cut(s)	1198
Cac8I	GCNNGC	6 cut(s)	873, 889, 1087, 1112, 1124, 1497
CaiI	CAGNNNCTG	1 cut(s)	1973
CfoI	GCGC	2 cut(s)	361, 1593
Cfr10I	RCCGGY	1 cut(s)	355
Cfr13I	GGNCC	7 cut(s)	189, 1055, 1187, 1556, 1880, 1977, 1978
Cfr9I	CCCGGG	1 cut(s)	1360
CseI	GACGC	1 cut(s)	1554
Csp6I	GTAC	5 cut(s)	364, 656, 1009, 1691, 1991
CspCI	CAANNNNNGTGG	4 cut(s)	359, 394, 974, 1009
CviQI	GTAC	5 cut(s)	364, 656, 1009, 1691, 1991
DdeI	CTNAG	3 cut(s)	212, 1694, 1815
DpnI	GATC	8 cut(s)	699, 1140, 1335, 1674, 1725, 1743, 1965, 1986
DpnII	GATC	8 cut(s)	697, 1138, 1333, 1672, 1723, 1741, 1963, 1984
DraIII	CACNNNGTG	1 cut(s)	605
EaeI	YGGCCR	3 cut(s)	367, 1124, 2095
Eam1104I	CTCTTC	4 cut(s)	33, 69, 960, 1093
EarI	CTCTTC	4 cut(s)	33, 69, 960, 1093
EciI	GGCGGA	1 cut(s)	165
Eco130I	CCWWGG	4 cut(s)	930, 1093, 1473, 2092
Eco24I	GRGCYC	1 cut(s)	1981
Eco47I	GGWCC	4 cut(s)	189, 1187, 1556, 1880
Eco57I	CTGAAG	2 cut(s)	984, 1095
Eco81I	CCTNAGG	1 cut(s)	212
Eco88I	CYCGRG	1 cut(s)	1360
EcoNI	CCTNNNNNAGG	1 cut(s)	1395
EcoO109I	RGGNCCY	4 cut(s)	1187, 1880, 1977, 1978
EcoRII	CCWGG	3 cut(s)	185, 1057, 2075
EcoT14I	CCWWGG	4 cut(s)	930, 1093, 1473, 2092
EcoT22I	ATGCAT	1 cut(s)	1282
EcoT38I	GRGCYC	1 cut(s)	1981
ErhI	CCWWGG	4 cut(s)	930, 1093, 1473, 2092
FalI	AAGNNNNNCTT	4 cut(s)	505, 537, 1520, 1552
FaqI	GGGAC	4 cut(s)	186, 773, 1200, 1343
FauI	CCCGC	2 cut(s)	1094, 1586
FbaI	TGATCA	3 cut(s)	1138, 1741, 1984
FblI	GTMKAC	1 cut(s)	2002
Fnu4HI	GCNGC	4 cut(s)	83, 1090, 1508, 1686
FokI	GGATG	5 cut(s)	830, 965, 1352, 1652, 1836
FriOI	GRGCYC	1 cut(s)	1981
Fsp4HI	GCNGC	4 cut(s)	83, 1090, 1508, 1686
FspBI	CTAG	2 cut(s)	266, 1070
GlaI	GCGC	2 cut(s)	360, 1592
GluI	GCNGC	4 cut(s)	83, 1090, 1508, 1686
GsaI	CCCAGC	1 cut(s)	1977
GsuI	CTGGAG	1 cut(s)	1532
HaeIII	GGCC	6 cut(s)	369, 503, 1057, 1126, 1979, 2097
HapII	CCGG	2 cut(s)	356, 1361
HgaI	GACGC	1 cut(s)	1554
HhaI	GCGC	2 cut(s)	361, 1593
Hin6I	GCGC	2 cut(s)	359, 1591
HinP1I	GCGC	2 cut(s)	359, 1591
HincII	GTYRAC	1 cut(s)	1371
HindII	GTYRAC	1 cut(s)	1371
HindIII	AAGCTT	2 cut(s)	35, 869
HinfI	GANTC	5 cut(s)	156, 401, 1022, 1116, 1643
HpaII	CCGG	2 cut(s)	356, 1361
HphI	GGTGA	4 cut(s)	410, 1376, 1613, 2008
Hpy166II	GTNNAC	7 cut(s)	96, 353, 1371, 1712, 1754, 1913, 2003
Hpy188I	TCNGA	6 cut(s)	406, 631, 702, 1250, 1293, 1788
Hpy188III	TCNNGA	8 cut(s)	214, 758, 944, 1256, 1337, 1670, 1961, 1967
Hpy8I	GTNNAC	7 cut(s)	96, 353, 1371, 1712, 1754, 1913, 2003
HpyAV	CCTTC	7 cut(s)	132, 316, 781, 848, 1195, 1436, 1658
HpyCH4III	ACNGT	3 cut(s)	655, 1809, 1860
HpyCH4IV	ACGT	2 cut(s)	1237, 1750
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1685, 1705
HpyF3I	CTNAG	3 cut(s)	212, 1694, 1815
HpySE526I	ACGT	2 cut(s)	1237, 1750
HspAI	GCGC	2 cut(s)	359, 1591
Ksp22I	TGATCA	3 cut(s)	1138, 1741, 1984
Kzo9I	GATC	8 cut(s)	697, 1138, 1333, 1672, 1723, 1741, 1963, 1984
LguI	GCTCTTC	2 cut(s)	69, 960
LmnI	GCTCC	3 cut(s)	631, 928, 1492
Lsp1109I	GCAGC	3 cut(s)	94, 1494, 1697
LweI	GCATC	5 cut(s)	1097, 1150, 1408, 1779, 1828
MaeI	CTAG	2 cut(s)	266, 1070
MaeII	ACGT	2 cut(s)	1237, 1750
MaeIII	GTNAC	2 cut(s)	1378, 1931
MalI	GATC	8 cut(s)	699, 1140, 1335, 1674, 1725, 1743, 1965, 1986
MboI	GATC	8 cut(s)	697, 1138, 1333, 1672, 1723, 1741, 1963, 1984
MflI	RGATCY	2 cut(s)	1333, 1963
MhlI	GDGCHC	3 cut(s)	925, 1915, 1981
MlsI	TGGCCA	2 cut(s)	1126, 2097
MluNI	TGGCCA	2 cut(s)	1126, 2097
MlyI	GAGTC	2 cut(s)	150, 1125
MmeI	TCCRAC	1 cut(s)	1433
Mox20I	TGGCCA	2 cut(s)	1126, 2097
Mph1103I	ATGCAT	1 cut(s)	1282
MroXI	GAANNNNTTC	1 cut(s)	1532
MscI	TGGCCA	2 cut(s)	1126, 2097
MseI	TTAA	5 cut(s)	374, 1013, 1275, 1607, 2084
MslI	CAYNNNNRTG	1 cut(s)	246
Msp20I	TGGCCA	2 cut(s)	1126, 2097
MspA1I	CMGCKG	1 cut(s)	1122
MspI	CCGG	2 cut(s)	356, 1361
MspR9I	CCNGG	5 cut(s)	187, 1059, 1361, 1362, 2077
Mva1269I	GAATGC	1 cut(s)	1222
MvaI	CCWGG	3 cut(s)	187, 1059, 2077
MvnI	CGCG	1 cut(s)	1593
MwoI	GCNNNNNNNGC	2 cut(s)	1685, 1705
NciI	CCSGG	2 cut(s)	1361, 1362
NcoI	CCATGG	2 cut(s)	1473, 2092
NdeII	GATC	8 cut(s)	697, 1138, 1333, 1672, 1723, 1741, 1963, 1984
NlaIV	GGNNCC	7 cut(s)	76, 597, 1188, 1881, 1965, 1978, 1979
NmuCI	GTSAC	1 cut(s)	1931
NsiI	ATGCAT	1 cut(s)	1282
NspI	RCATGY	2 cut(s)	245, 1499
PaeI	GCATGC	1 cut(s)	1499
PciSI	GCTCTTC	2 cut(s)	69, 960
PctI	GAATGC	1 cut(s)	1222
PdmI	GAANNNNTTC	1 cut(s)	1532
PfeI	GAWTC	3 cut(s)	401, 1022, 1643
PflMI	CCANNNNNTGG	1 cut(s)	1973
PkrI	GCNGC	4 cut(s)	84, 1091, 1509, 1687
PleI	GAGTC	2 cut(s)	150, 1124
PpsI	GAGTC	2 cut(s)	150, 1124
Ppu21I	YACGTR	1 cut(s)	1238
PpuMI	RGGWCCY	2 cut(s)	1187, 1880
PshBI	ATTAAT	1 cut(s)	1275
PsiI	TTATAA	1 cut(s)	528
Psp5II	RGGWCCY	2 cut(s)	1187, 1880
Psp6I	CCWGG	3 cut(s)	185, 1057, 2075
PspFI	CCCAGC	1 cut(s)	1973
PspGI	CCWGG	3 cut(s)	185, 1057, 2075
PspN4I	GGNNCC	7 cut(s)	76, 597, 1188, 1881, 1965, 1978, 1979
PspOMI	GGGCCC	1 cut(s)	1977
PspPI	GGNCC	7 cut(s)	189, 1055, 1187, 1556, 1880, 1977, 1978
PspPPI	RGGWCCY	2 cut(s)	1187, 1880
PsrI	GAACNNNNNNTAC	2 cut(s)	1371, 1403
PstNI	CAGNNNCTG	1 cut(s)	1973
PsuI	RGATCY	2 cut(s)	1333, 1963
PvuII	CAGCTG	1 cut(s)	1122
RsaI	GTAC	5 cut(s)	365, 657, 1010, 1692, 1992
RsaNI	GTAC	5 cut(s)	364, 656, 1009, 1691, 1991
RseI	CAYNNNNRTG	1 cut(s)	246
SapI	GCTCTTC	2 cut(s)	69, 960
SaqAI	TTAA	5 cut(s)	374, 1013, 1275, 1607, 2084
SatI	GCNGC	4 cut(s)	83, 1090, 1508, 1686
Sau3AI	GATC	8 cut(s)	697, 1138, 1333, 1672, 1723, 1741, 1963, 1984
Sau96I	GGNCC	7 cut(s)	189, 1055, 1187, 1556, 1880, 1977, 1978
ScaI	AGTACT	2 cut(s)	1010, 1692
SchI	GAGTC	2 cut(s)	150, 1125
ScrFI	CCNGG	5 cut(s)	187, 1059, 1361, 1362, 2077
SduI	GDGCHC	3 cut(s)	925, 1915, 1981
SfaNI	GCATC	5 cut(s)	1097, 1150, 1408, 1779, 1828
SfcI	CTRYAG	1 cut(s)	1374
SinI	GGWCC	4 cut(s)	189, 1187, 1556, 1880
SmaI	CCCGGG	1 cut(s)	1362
SmiMI	CAYNNNNRTG	1 cut(s)	246
SphI	GCATGC	1 cut(s)	1499
SsiI	CCGC	5 cut(s)	176, 1087, 1090, 1311, 1593
SspMI	CTAG	2 cut(s)	266, 1070
StyD4I	CCNGG	5 cut(s)	185, 1057, 1359, 1360, 2075
StyI	CCWWGG	4 cut(s)	930, 1093, 1473, 2092
TaaI	ACNGT	3 cut(s)	655, 1809, 1860
TaiI	ACGT	2 cut(s)	1240, 1753
TaqI	TCGA	2 cut(s)	22, 1954
TatI	WGTACW	4 cut(s)	655, 1008, 1690, 1990
TauI	GCSGC	1 cut(s)	1092
TfiI	GAWTC	3 cut(s)	401, 1022, 1643
Tru1I	TTAA	5 cut(s)	374, 1013, 1275, 1607, 2084
Tru9I	TTAA	5 cut(s)	374, 1013, 1275, 1607, 2084
TscAI	CASTG	4 cut(s)	64, 467, 582, 1048
TseFI	GTSAC	1 cut(s)	1931
TseI	GCWGC	3 cut(s)	82, 1507, 1685
Tsp45I	GTSAC	1 cut(s)	1931
TspDTI	ATGAA	7 cut(s)	8, 20, 357, 1284, 1455, 1909, 1940
TspMI	CCCGGG	1 cut(s)	1360
TspRI	CASTG	4 cut(s)	64, 467, 582, 1048
Van91I	CCANNNNNTGG	1 cut(s)	1973
VneI	GTGCAC	1 cut(s)	1911
VpaK11BI	GGWCC	4 cut(s)	189, 1187, 1556, 1880
VspI	ATTAAT	1 cut(s)	1275
XagI	CCTNNNNNAGG	1 cut(s)	1395
XapI	RAATTY	3 cut(s)	1457, 1583, 2009
XceI	RCATGY	2 cut(s)	245, 1499
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1659
XmaI	CCCGGG	1 cut(s)	1360
XmiI	GTMKAC	1 cut(s)	2002
XmnI	GAANNNNTTC	1 cut(s)	1532
XspI	CTAG	2 cut(s)	266, 1070
ZrmI	AGTACT	2 cut(s)	1010, 1692
Zsp2I	ATGCAT	1 cut(s)	1282

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.