Rorug05G0040700

Primase homolog protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001578
Physical Location & Seq
Forward (+)
3505566 .. 3506859
1294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0040700.1

Sequence Viewer

Length: 1101 bp
ATGGACATGAACGTGGTGTTCTCGCCGCTGTCCATCCATGTGGTTCTTAGCATGATAGCAGCTGGCACCAAGGGTTTCAACCAAGAAGAGATGCTCAATTTCCTCAAGTCCAAGTCCATACACGAGCTCAACACTCTCGCCTCCAATGTCATCCCCCTAGTCTTTGCCGATGGATCTGAACACGGCGGGCCTCTCTTGTCATTTGCCAATGGGGTTTGGATAGATGAATCTCTCTCCATCAAGCCTTCTTTCAAAAATGTACTGGATACTGATTACAAGGCGACCTTGAAACAAGTTGATTTCAAGACTGAGCCAGAGGAAGTGCGACATAAAGTGAATTCCTGGGGGGAAAAGAAGACCAATGGTCTCATCAAAAGCATTCTTCCCCCTATGTCTGTTTCTCGCACAACAAGGCTCATTCTTGCAAATGCTGTATACTTTAAAGGAGAATGGGATGAGGAGTTTGATGCATCAGAGACAAGAGTGTACAAATTTAACCTTCTCCATAATGGGGGCTCAGTTAAGGCACGCTTCATGACAAAGTCGTCGAAACAGTTTATTAGTGTCTTTCAAAGCTTCAAAGTCTTGAAGCTTCCCTACAAACAAGGTAAAGATCATGAGCGAATGTTCTCCATGTACATTTTTCTTCCAAATGCAAGGAACGGGCTGCGAGCGTTGGTCGAGAAGTTTTGCTCCAAGTTTGAGTTCATAGATAGCCATCTTCCTTACATAGAAGTTCCAGTGGGTGAATTCTTAATCCCAAAGTTTAAGATTTCCTGTGGTTTTGAGGCTTCCAAACTTCTGGAGACTTTGGGATTCTCTCCTGGAAGTTTGACAGAGATGGTGGACTCACCTGAGCCCCTACATGTTTCCAACATATTCCACAAAGCCTTCATTGAAGTTAATGAAAAAGGCACAGAAGCTGCTGCTGTAACGGCTTTTGTTGTGACGACGTGCTCGTTCCCACCCCGGCCCCTTCCTGATCCGATAGATTTTGTGGCAGATCACCCATTCCTTTATCTCATCAGAGAAGAAGTGACTAAAACGGTCATGTTCGTTGGGCAGGTCCTCAATCCTATTGAAGACTTGTTTATCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Toprim_4

Protein Analysis

366

Amino Acids

41.19

Weight (kDa)

6.23

Isoelectric Point (pI)

32.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serpin PF00079 3 - 359 4.3e-86 Serpin (serine protease inhibitor)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 544
Acc36I ACCTGC 1 cut(s) 1054
AccB1I GGYRCC 1 cut(s) 65
AccI GTMKAC 1 cut(s) 435
AciI CCGC 2 cut(s) 26, 186
AclWI GGATC 2 cut(s) 181, 977
AcsI RAATTY 3 cut(s) 337, 491, 749
AfaI GTAC 3 cut(s) 261, 488, 638
AfiI CCNNNNNNNGG 1 cut(s) 511
AflIII ACRYGT 1 cut(s) 865
AgsI TTSAA 9 cut(s) 79, 253, 289, 304, 572, 580, 589, 899, 1082
AhdI GACNNNNNGTC 1 cut(s) 363
AjiI CACGTC 1 cut(s) 954
AjnI CCWGG 2 cut(s) 341, 823
AjuI GAANNNNNNNTTGG 2 cut(s) 689, 721
AluBI AGCT 5 cut(s) 62, 127, 576, 592, 923
AluI AGCT 5 cut(s) 62, 127, 576, 592, 923
Alw21I GWGCWC 2 cut(s) 129, 959
Alw26I GTCTC 3 cut(s) 371, 470, 800
AlwI GGATC 2 cut(s) 181, 977
AlwNI CAGNNNCTG 1 cut(s) 923
AoxI GGCC 2 cut(s) 188, 971
ApeKI GCWGC 4 cut(s) 59, 667, 923, 926
ApoI RAATTY 3 cut(s) 337, 491, 749
AspS9I GGNCC 3 cut(s) 188, 972, 1066
AsuC2I CCSGG 1 cut(s) 970
AsuHPI GGTGA 3 cut(s) 758, 843, 998
AvaII GGWCC 1 cut(s) 1066
BanI GGYRCC 1 cut(s) 65
BanII GRGCYC 3 cut(s) 129, 518, 861
BarI GAAGNNNNNNTAC 2 cut(s) 581, 613
BauI CACGAG 1 cut(s) 122
BbsI GAAGAC 2 cut(s) 362, 1089
Bbv12I GWGCWC 2 cut(s) 129, 959
BbvI GCAGC 4 cut(s) 71, 654, 910, 913
BccI CCATC 5 cut(s) 41, 164, 245, 726, 835
BceAI ACGGC 2 cut(s) 199, 951
BciT130I CCWGG 2 cut(s) 343, 825
BciVI GTATCC 1 cut(s) 259
BcnI CCSGG 1 cut(s) 970
BcoDI GTCTC 3 cut(s) 371, 470, 800
BfaI CTAG 2 cut(s) 158, 1099
BfuAI ACCTGC 1 cut(s) 1054
BfuI GTATCC 1 cut(s) 259
BisI GCNGC 5 cut(s) 26, 60, 668, 924, 927
BlsI GCNGC 5 cut(s) 27, 61, 669, 925, 928
Bme1390I CCNGG 3 cut(s) 343, 825, 970
Bme18I GGWCC 1 cut(s) 1066
BmeRI GACNNNNNGTC 1 cut(s) 363
BmgBI CACGTC 1 cut(s) 954
BmgT120I GGNCC 3 cut(s) 188, 972, 1066
BmiI GGNNCC 2 cut(s) 67, 974
BmrFI CCNGG 3 cut(s) 343, 825, 970
BmsI GCATC 3 cut(s) 81, 457, 479
BpiI GAAGAC 2 cut(s) 362, 1089
BpmI CTGGAG 1 cut(s) 824
Bpu10I CCTNAGC 1 cut(s) 855
BpuEI CTTGAG 1 cut(s) 89
BpuMI CCSGG 1 cut(s) 970
BsaBI GATNNNNATC 1 cut(s) 717
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 3 cut(s) 69, 342, 968
Bsc4I CCNNNNNNNGG 1 cut(s) 511
Bse1I ACTGG 2 cut(s) 267, 740
Bse8I GATNNNNATC 1 cut(s) 717
BseBI CCWGG 2 cut(s) 343, 825
BseDI CCNNGG 3 cut(s) 69, 342, 968
BseGI GGATG 3 cut(s) 33, 150, 460
BseJI GATNNNNATC 1 cut(s) 717
BseLI CCNNNNNNNGG 1 cut(s) 511
BseMII CTCAG 3 cut(s) 300, 531, 846
BseNI ACTGG 2 cut(s) 267, 740
BseRI GAGGAG 1 cut(s) 473
BseXI GCAGC 4 cut(s) 71, 654, 910, 913
BshFI GGCC 2 cut(s) 190, 973
BshNI GGYRCC 1 cut(s) 65
BsiHKAI GWGCWC 2 cut(s) 129, 959
BsiSI CCGG 1 cut(s) 970
BslI CCNNNNNNNGG 1 cut(s) 511
BsmAI GTCTC 3 cut(s) 371, 470, 800
BsmI GAATGC 1 cut(s) 378
BsnI GGCC 2 cut(s) 190, 973
Bso31I GGTCTC 1 cut(s) 371
Bsp1286I GDGCHC 4 cut(s) 129, 518, 861, 959
Bsp1407I TGTACA 2 cut(s) 486, 636
Bsp143I GATC 4 cut(s) 173, 613, 982, 1003
BspACI CCGC 2 cut(s) 26, 186
BspANI GGCC 2 cut(s) 190, 973
BspCNI CTCAG 3 cut(s) 301, 530, 847
BspHI TCATGA 2 cut(s) 534, 616
BspLI GGNNCC 2 cut(s) 67, 974
BspMI ACCTGC 1 cut(s) 1054
BspPI GGATC 2 cut(s) 181, 977
BspT107I GGYRCC 1 cut(s) 65
BspTNI GGTCTC 1 cut(s) 371
BsrGI TGTACA 2 cut(s) 486, 636
BsrI ACTGG 2 cut(s) 267, 740
BssECI CCNNGG 3 cut(s) 69, 342, 968
BssMI GATC 4 cut(s) 173, 613, 982, 1003
BssNAI GTATAC 1 cut(s) 436
BssSI CACGAG 1 cut(s) 122
BssT1I CCWWGG 1 cut(s) 69
Bst1107I GTATAC 1 cut(s) 436
Bst2BI CACGAG 1 cut(s) 122
Bst2UI CCWGG 2 cut(s) 343, 825
Bst4CI ACNGT 2 cut(s) 555, 1048
Bst6I CTCTTC 1 cut(s) 81
BstAUI TGTACA 2 cut(s) 486, 636
BstC8I GCNNGC 4 cut(s) 64, 188, 529, 672
BstDEI CTNAG 4 cut(s) 47, 309, 517, 855
BstF5I GGATG 3 cut(s) 33, 150, 460
BstKTI GATC 4 cut(s) 176, 616, 985, 1006
BstMAI GTCTC 3 cut(s) 371, 470, 800
BstMBI GATC 4 cut(s) 173, 613, 982, 1003
BstMWI GCNNNNNNNGC 1 cut(s) 935
BstNI CCWGG 2 cut(s) 343, 825
BstNSI RCATGY 1 cut(s) 869
BstSCI CCNGG 3 cut(s) 341, 823, 968
BstV1I GCAGC 4 cut(s) 71, 654, 910, 913
BstV2I GAAGAC 2 cut(s) 362, 1089
BstX2I RGATCY 1 cut(s) 173
BstXI CCANNNNNNTGG 2 cut(s) 40, 802
BstYI RGATCY 1 cut(s) 173
BstZ17I GTATAC 1 cut(s) 436
BsuI GTATCC 1 cut(s) 259
BsuRI GGCC 2 cut(s) 190, 973
BtrI CACGTC 1 cut(s) 954
BtsCI GGATG 3 cut(s) 33, 150, 460
BtsIMutI CAGTG 1 cut(s) 747
BveI ACCTGC 1 cut(s) 1054
Cac8I GCNNGC 4 cut(s) 64, 188, 529, 672
CaiI CAGNNNCTG 1 cut(s) 923
CciI TCATGA 2 cut(s) 534, 616
Cfr13I GGNCC 3 cut(s) 188, 972, 1066
Csp6I GTAC 3 cut(s) 260, 487, 637
CviAII CATG 8 cut(s) 7, 38, 52, 535, 617, 634, 866, 1051
CviQI GTAC 3 cut(s) 260, 487, 637
DdeI CTNAG 4 cut(s) 47, 309, 517, 855
DpnI GATC 4 cut(s) 175, 615, 984, 1005
DpnII GATC 4 cut(s) 173, 613, 982, 1003
DraI TTTAAA 1 cut(s) 442
DrdI GACNNNNNNGTC 1 cut(s) 544
DriI GACNNNNNGTC 1 cut(s) 363
DseDI GACNNNNNNGTC 1 cut(s) 544
Eam1104I CTCTTC 1 cut(s) 81
Eam1105I GACNNNNNGTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 81
Ecl136II GAGCTC 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 69
Eco24I GRGCYC 3 cut(s) 129, 518, 861
Eco31I GGTCTC 1 cut(s) 371
Eco47I GGWCC 1 cut(s) 1066
Eco53kI GAGCTC 1 cut(s) 127
EcoICRI GAGCTC 1 cut(s) 127
EcoO109I RGGNCCY 1 cut(s) 1066
EcoRI GAATTC 2 cut(s) 337, 749
EcoRII CCWGG 2 cut(s) 341, 823
EcoT14I CCWWGG 1 cut(s) 69
EcoT22I ATGCAT 1 cut(s) 472
EcoT38I GRGCYC 3 cut(s) 129, 518, 861
ErhI CCWWGG 1 cut(s) 69
FaeI CATG 8 cut(s) 10, 41, 55, 538, 620, 637, 869, 1054
FalI AAGNNNNNCTT 4 cut(s) 269, 301, 515, 547
FatI CATG 8 cut(s) 6, 37, 51, 534, 616, 633, 865, 1050
FauI CCCGC 1 cut(s) 179
FblI GTMKAC 1 cut(s) 435
Fnu4HI GCNGC 5 cut(s) 26, 60, 668, 924, 927
FokI GGATG 3 cut(s) 20, 137, 467
FriOI GRGCYC 3 cut(s) 129, 518, 861
Fsp4HI GCNGC 5 cut(s) 26, 60, 668, 924, 927
FspBI CTAG 2 cut(s) 158, 1099
GluI GCNGC 5 cut(s) 26, 60, 668, 924, 927
GsuI CTGGAG 1 cut(s) 824
HaeIII GGCC 2 cut(s) 190, 973
HapII CCGG 1 cut(s) 970
Hin1II CATG 8 cut(s) 10, 41, 55, 538, 620, 637, 869, 1054
HindIII AAGCTT 2 cut(s) 574, 590
HinfI GANTC 3 cut(s) 227, 816, 848
HpaII CCGG 1 cut(s) 970
HphI GGTGA 3 cut(s) 758, 843, 998
Hpy166II GTNNAC 3 cut(s) 436, 487, 847
Hpy188I TCNGA 4 cut(s) 178, 475, 987, 1028
Hpy188III TCNNGA 7 cut(s) 304, 535, 586, 617, 682, 803, 980
Hpy8I GTNNAC 3 cut(s) 436, 487, 847
Hpy99I CGWCG 2 cut(s) 550, 955
HpyAV CCTTC 4 cut(s) 255, 509, 901, 986
HpyCH4III ACNGT 2 cut(s) 555, 1048
HpyCH4IV ACGT 2 cut(s) 12, 953
HpyCH4V TGCA 3 cut(s) 425, 470, 656
HpyF10VI GCNNNNNNNGC 1 cut(s) 935
HpyF3I CTNAG 4 cut(s) 47, 309, 517, 855
HpySE526I ACGT 2 cut(s) 12, 953
Hsp92II CATG 8 cut(s) 10, 41, 55, 538, 620, 637, 869, 1054
Kzo9I GATC 4 cut(s) 173, 613, 982, 1003
LmnI GCTCC 1 cut(s) 698
Lsp1109I GCAGC 4 cut(s) 71, 654, 910, 913
LweI GCATC 3 cut(s) 81, 457, 479
MaeI CTAG 2 cut(s) 158, 1099
MaeII ACGT 2 cut(s) 12, 953
MaeIII GTNAC 3 cut(s) 931, 946, 1036
MalI GATC 4 cut(s) 175, 615, 984, 1005
MboI GATC 4 cut(s) 173, 613, 982, 1003
MboII GAAGA 7 cut(s) 98, 367, 374, 638, 713, 1043, 1094
MflI RGATCY 1 cut(s) 173
MhlI GDGCHC 4 cut(s) 129, 518, 861, 959
MluCI AATT 4 cut(s) 97, 337, 491, 749
MlyI GAGTC 1 cut(s) 842
MmeI TCCRAC 1 cut(s) 897
MnlI CCTC 7 cut(s) 113, 151, 201, 310, 451, 781, 1079
Mph1103I ATGCAT 1 cut(s) 472
MseI TTAA 6 cut(s) 441, 495, 522, 755, 768, 903
MslI CAYNNNNRTG 2 cut(s) 11, 38
MspA1I CMGCKG 2 cut(s) 28, 62
MspI CCGG 1 cut(s) 970
MspR9I CCNGG 3 cut(s) 343, 825, 970
Mva1269I GAATGC 1 cut(s) 378
MvaI CCWGG 2 cut(s) 343, 825
MwoI GCNNNNNNNGC 1 cut(s) 935
NciI CCSGG 1 cut(s) 970
NdeII GATC 4 cut(s) 173, 613, 982, 1003
NlaIII CATG 8 cut(s) 10, 41, 55, 538, 620, 637, 869, 1054
NlaIV GGNNCC 2 cut(s) 67, 974
NmuCI GTSAC 2 cut(s) 946, 1036
NsiI ATGCAT 1 cut(s) 472
NspI RCATGY 1 cut(s) 869
PagI TCATGA 2 cut(s) 534, 616
PciI ACATGT 1 cut(s) 865
PcsI WCGNNNNNNNCGW 1 cut(s) 956
PctI GAATGC 1 cut(s) 378
PfeI GAWTC 2 cut(s) 227, 816
PflFI GACNNNGTC 1 cut(s) 541
PfoI TCCNGGA 1 cut(s) 823
PkrI GCNGC 5 cut(s) 27, 61, 669, 925, 928
PleI GAGTC 1 cut(s) 842
PpsI GAGTC 1 cut(s) 842
PpuMI RGGWCCY 1 cut(s) 1066
PscI ACATGT 1 cut(s) 865
Psp124BI GAGCTC 1 cut(s) 129
Psp5II RGGWCCY 1 cut(s) 1066
Psp6I CCWGG 2 cut(s) 341, 823
PspGI CCWGG 2 cut(s) 341, 823
PspN4I GGNNCC 2 cut(s) 67, 974
PspPI GGNCC 3 cut(s) 188, 972, 1066
PspPPI RGGWCCY 1 cut(s) 1066
PstNI CAGNNNCTG 1 cut(s) 923
PsuI RGATCY 1 cut(s) 173
PsyI GACNNNGTC 1 cut(s) 541
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 3 cut(s) 261, 488, 638
RsaNI GTAC 3 cut(s) 260, 487, 637
RseI CAYNNNNRTG 2 cut(s) 11, 38
SacI GAGCTC 1 cut(s) 129
SaqAI TTAA 6 cut(s) 441, 495, 522, 755, 768, 903
SatI GCNGC 5 cut(s) 26, 60, 668, 924, 927
Sau3AI GATC 4 cut(s) 173, 613, 982, 1003
Sau96I GGNCC 3 cut(s) 188, 972, 1066
SchI GAGTC 1 cut(s) 842
ScrFI CCNGG 3 cut(s) 343, 825, 970
SduI GDGCHC 4 cut(s) 129, 518, 861, 959
SfaNI GCATC 3 cut(s) 81, 457, 479
SinI GGWCC 1 cut(s) 1066
SmiMI CAYNNNNRTG 2 cut(s) 11, 38
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 4 cut(s) 97, 337, 491, 749
SsiI CCGC 2 cut(s) 26, 186
SspMI CTAG 2 cut(s) 158, 1099
SstI GAGCTC 1 cut(s) 129
StyD4I CCNGG 3 cut(s) 341, 823, 968
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 2 cut(s) 555, 1048
TaiI ACGT 2 cut(s) 15, 956
TaqI TCGA 2 cut(s) 548, 681
TasI AATT 4 cut(s) 97, 337, 491, 749
TatI WGTACW 3 cut(s) 259, 486, 636
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 2 cut(s) 227, 816
Tru1I TTAA 6 cut(s) 441, 495, 522, 755, 768, 903
Tru9I TTAA 6 cut(s) 441, 495, 522, 755, 768, 903
TscAI CASTG 1 cut(s) 747
TseFI GTSAC 2 cut(s) 946, 1036
TseI GCWGC 4 cut(s) 59, 667, 923, 926
Tsp45I GTSAC 2 cut(s) 946, 1036
TspDTI ATGAA 6 cut(s) 23, 240, 523, 697, 883, 921
TspRI CASTG 1 cut(s) 747
Tth111I GACNNNGTC 1 cut(s) 541
VpaK11BI GGWCC 1 cut(s) 1066
XapI RAATTY 3 cut(s) 337, 491, 749
XceI RCATGY 1 cut(s) 869
XmiI GTMKAC 1 cut(s) 435
XspI CTAG 2 cut(s) 158, 1099
Zsp2I ATGCAT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.