Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002901

Physical Location & Seq

Genomic Coordinates Reverse (-)

38398923 .. 38403632

Gene Structure 4710 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1296100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 744 bp

ATGTACATAGCTTCTATGCGAAAGTCGTTCAAGGACTCTCTGAAAGTTCTTGAAGCTGATATTCAGCATGCAAATACTCTGGCCTCAGATTTTCCGAGGGAGTATGATGGTGCTTGTGTTCAGATGAGAATGTCATACAGTCCAGCCGCTCATCTCTTCCTTTTTCTGGTGCAATGGACTGACTGCAACCTTGCAGGAGCCCTTGGGCTGTTAAGAATTCTAATTTACAAGGTTTATGTGGATGGCTCAACCACCATGTCTACCAAGGAAAGGAAAGCAAGCATCAGAGAGTTCTATGCTGTTATTTATCCCTCTTTATTGCAACTTCAAGGAGGTGTCACCGATAGTGAAGATAAAAGGCAGAAGGCAGTGTGCCTGGAAAGGTATTGCAAAAGAGATGATGAGGAAAGTCGACAGTGTTCAGATATCGACATTGAAAGAGAAGAGGAATGCGGTATATGCATGGAGACAAATAGTAAAATTGTATTGCCCAACTGCAACCATGTTTTGTGCCTGAAATGTTACCGTGATTGGCGTTCAAGATCTCAGTCGTGCCCTTTCTGTCGTGACAATCTTAAAAGAGTGAACTCAGGCGATCTCTGGGTCTACACCGACAGCAGGGACATAATTGACATGGAAACAGTGATGACGGAGAATCTCAGGAGGCTTTTCACGTATATAGATAAGTTGCCTCTTATTACACCGGATAACCCTTTTGACTCGTATGATTCCCACCTAAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0051865 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Prok-RING_4 zf-C3HC4_3 zf-RING_2

Protein Analysis

248

Amino Acids

28.69

Weight (kDa)

5.67

Isoelectric Point (pI)

58.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_3	PF13920	148 - 194	4.6e-11	Zinc finger, C3HC4 type (RING finger)
zf-RING_2	PF13639	150 - 189	1.2e-08	Ring finger domain
zf-RING_5	PF14634	150 - 189	7.2e-06	zinc-RING finger domain
Prok-RING_4	PF14447	151 - 194	3.6e-07	Prokaryotic RING finger family 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002901)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01520 AT5G01520
fragaria_vesca	FvH4_3g04920 FvH4_6g11510 FvH4_6g11510
malus_domestica	MD04G1151400.v1.1 MD05G1314700.v1.1 MD10G1296100.v1.1 MD12G1165500.v1.1
prunus_persica	Prupe.4G044500_v2.0.a1 Prupe.6G276500_v2.0.a1 Prupe.6G276500_v2.0.a1 Prupe.6G276500_v2.0.a1
pyrus_communis	pycom04g13730 pycom05g29250 pycom10g24860 pycom12g15810
rosa_chinensis	RchiOBHm_Chr3g0463221 RchiOBHm_Chr5g0008081
rosa_laevigata	RLG00000024801 RLG00000031534
rosa_multiflora	Rmu_sc0001626.1_g000003 Rmu_sc0004643.1_g000001 Rmu_sc0017465.1_g000004
rosa_roxburghii	Rroxscaffold_1G00068400 Rroxscaffold_6G00416790
rosa_rugosa	Rorug03G0062500 Rorug03G0062500 Rorug04G0433300
rosa_samantha	Rh3AG120700 Rh3BG125100 Rh3CG127200 Rh3DG126300 Rh5BG058100 Rh5CG068200 Rh5DG057300
rosa_wichuraiana	Rw3G010250 Rw5G005930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	149
AccI	GTMKAC	3 cut(s)	260, 412, 606
AciI	CCGC	2 cut(s)	147, 453
AcsI	RAATTY	1 cut(s)	216
AfaI	GTAC	1 cut(s)	5
AfiI	CCNNNNNNNGG	4 cut(s)	166, 270, 618, 738
AgsI	TTSAA	5 cut(s)	31, 53, 329, 437, 540
AjnI	CCWGG	1 cut(s)	375
AluBI	AGCT	2 cut(s)	11, 56
AluI	AGCT	2 cut(s)	11, 56
Alw26I	GTCTC	1 cut(s)	461
AoxI	GGCC	1 cut(s)	81
ApoI	RAATTY	1 cut(s)	216
AsuHPI	GGTGA	1 cut(s)	331
AxyI	CCTNAGG	1 cut(s)	737
BaeGI	GKGCMC	1 cut(s)	557
BanII	GRGCYC	1 cut(s)	202
BccI	CCATC	2 cut(s)	101, 236
BciT130I	CCWGG	1 cut(s)	377
BcoDI	GTCTC	1 cut(s)	461
BglII	AGATCT	1 cut(s)	542
BisI	GCNGC	1 cut(s)	147
BlsI	GCNGC	1 cut(s)	148
Bme1390I	CCNGG	1 cut(s)	377
BmiI	GGNNCC	1 cut(s)	199
BmrFI	CCNGG	1 cut(s)	377
BmsI	GCATC	1 cut(s)	291
BsaAI	YACGTR	1 cut(s)	675
BsaJI	CCNNGG	3 cut(s)	95, 202, 264
BsaWI	WCCGGW	1 cut(s)	703
Bsc4I	CCNNNNNNNGG	4 cut(s)	166, 270, 618, 738
Bse21I	CCTNAGG	1 cut(s)	737
Bse3DI	GCAATG	1 cut(s)	179
BseBI	CCWGG	1 cut(s)	377
BseDI	CCNNGG	3 cut(s)	95, 202, 264
BseGI	GGATG	1 cut(s)	247
BseLI	CCNNNNNNNGG	4 cut(s)	166, 270, 618, 738
BseMI	GCAATG	1 cut(s)	179
BseMII	CTCAG	4 cut(s)	99, 560, 603, 673
BseSI	GKGCMC	1 cut(s)	557
BshFI	GGCC	1 cut(s)	83
BsiSI	CCGG	1 cut(s)	704
BslFI	GGGAC	1 cut(s)	635
BslI	CCNNNNNNNGG	4 cut(s)	166, 270, 618, 738
BsmAI	GTCTC	1 cut(s)	461
BsmFI	GGGAC	1 cut(s)	635
BsmI	GAATGC	1 cut(s)	455
BsnI	GGCC	1 cut(s)	83
Bsp1286I	GDGCHC	2 cut(s)	202, 557
Bsp1407I	TGTACA	1 cut(s)	3
Bsp143I	GATC	2 cut(s)	542, 595
BspACI	CCGC	2 cut(s)	147, 453
BspANI	GGCC	1 cut(s)	83
BspCNI	CTCAG	4 cut(s)	98, 559, 602, 672
BspLI	GGNNCC	1 cut(s)	199
BsrBI	CCGCTC	1 cut(s)	149
BsrDI	GCAATG	1 cut(s)	179
BsrGI	TGTACA	1 cut(s)	3
BssECI	CCNNGG	3 cut(s)	95, 202, 264
BssMI	GATC	2 cut(s)	542, 595
BssT1I	CCWWGG	2 cut(s)	202, 264
Bst2UI	CCWGG	1 cut(s)	377
Bst4CI	ACNGT	4 cut(s)	140, 417, 527, 643
Bst6I	CTCTTC	2 cut(s)	161, 438
BstAUI	TGTACA	1 cut(s)	3
BstBAI	YACGTR	1 cut(s)	675
BstC8I	GCNNGC	2 cut(s)	69, 280
BstDEI	CTNAG	5 cut(s)	85, 546, 589, 659, 737
BstF5I	GGATG	1 cut(s)	247
BstKTI	GATC	2 cut(s)	545, 598
BstMAI	GTCTC	1 cut(s)	461
BstMBI	GATC	2 cut(s)	542, 595
BstMWI	GCNNNNNNNGC	1 cut(s)	459
BstNI	CCWGG	1 cut(s)	377
BstNSI	RCATGY	1 cut(s)	71
BstSCI	CCNGG	1 cut(s)	375
BstSLI	GKGCMC	1 cut(s)	557
BstX2I	RGATCY	1 cut(s)	542
BstYI	RGATCY	1 cut(s)	542
Bsu36I	CCTNAGG	1 cut(s)	737
BsuRI	GGCC	1 cut(s)	83
BtsCI	GGATG	1 cut(s)	247
BtsI	GCAGTG	1 cut(s)	375
BtsIMutI	CAGTG	3 cut(s)	375, 422, 648
Cac8I	GCNNGC	2 cut(s)	69, 280
Csp6I	GTAC	1 cut(s)	4
CviAII	CATG	5 cut(s)	68, 256, 463, 503, 634
CviJI	RGCY	8 cut(s)	11, 56, 83, 146, 200, 208, 246, 667
CviKI_1	RGCY	8 cut(s)	11, 56, 83, 146, 200, 208, 246, 667
CviQI	GTAC	1 cut(s)	4
DdeI	CTNAG	5 cut(s)	85, 546, 589, 659, 737
DpnI	GATC	2 cut(s)	544, 597
DpnII	GATC	2 cut(s)	542, 595
Eam1104I	CTCTTC	2 cut(s)	161, 438
EarI	CTCTTC	2 cut(s)	161, 438
Eco130I	CCWWGG	2 cut(s)	202, 264
Eco24I	GRGCYC	1 cut(s)	202
Eco32I	GATATC	1 cut(s)	427
Eco81I	CCTNAGG	1 cut(s)	737
EcoRI	GAATTC	1 cut(s)	216
EcoRII	CCWGG	1 cut(s)	375
EcoRV	GATATC	1 cut(s)	427
EcoT14I	CCWWGG	2 cut(s)	202, 264
EcoT22I	ATGCAT	1 cut(s)	464
EcoT38I	GRGCYC	1 cut(s)	202
ErhI	CCWWGG	2 cut(s)	202, 264
FaeI	CATG	5 cut(s)	71, 259, 466, 506, 637
FaqI	GGGAC	1 cut(s)	635
FatI	CATG	5 cut(s)	67, 255, 462, 502, 633
FblI	GTMKAC	3 cut(s)	260, 412, 606
Fnu4HI	GCNGC	1 cut(s)	147
FokI	GGATG	1 cut(s)	254
FriOI	GRGCYC	1 cut(s)	202
Fsp4HI	GCNGC	1 cut(s)	147
GluI	GCNGC	1 cut(s)	147
HaeIII	GGCC	1 cut(s)	83
HapII	CCGG	1 cut(s)	704
Hin1II	CATG	5 cut(s)	71, 259, 466, 506, 637
HincII	GTYRAC	1 cut(s)	413
HindII	GTYRAC	1 cut(s)	413
HinfI	GANTC	4 cut(s)	35, 655, 719, 728
HpaII	CCGG	1 cut(s)	704
HphI	GGTGA	1 cut(s)	331
Hpy166II	GTNNAC	4 cut(s)	261, 413, 586, 607
Hpy188I	TCNGA	6 cut(s)	42, 88, 96, 123, 287, 424
Hpy188III	TCNNGA	4 cut(s)	50, 540, 566, 661
Hpy8I	GTNNAC	4 cut(s)	261, 413, 586, 607
HpyAV	CCTTC	1 cut(s)	358
HpyCH4III	ACNGT	4 cut(s)	140, 417, 527, 643
HpyCH4IV	ACGT	1 cut(s)	674
HpyCH4V	TGCA	8 cut(s)	71, 172, 186, 194, 322, 390, 462, 498
HpyF10VI	GCNNNNNNNGC	1 cut(s)	459
HpyF3I	CTNAG	5 cut(s)	85, 546, 589, 659, 737
HpySE526I	ACGT	1 cut(s)	674
Hsp92II	CATG	5 cut(s)	71, 259, 466, 506, 637
Kzo9I	GATC	2 cut(s)	542, 595
LmnI	GCTCC	1 cut(s)	197
LweI	GCATC	1 cut(s)	291
MaeII	ACGT	1 cut(s)	674
MaeIII	GTNAC	3 cut(s)	337, 521, 566
MalI	GATC	2 cut(s)	544, 597
MbiI	CCGCTC	1 cut(s)	149
MboI	GATC	2 cut(s)	542, 595
MboII	GAAGA	3 cut(s)	148, 362, 455
MflI	RGATCY	1 cut(s)	542
MhlI	GDGCHC	2 cut(s)	202, 557
MluCI	AATT	4 cut(s)	216, 222, 480, 627
MlyI	GAGTC	2 cut(s)	29, 713
MnlI	CCTC	8 cut(s)	90, 94, 322, 326, 397, 439, 657, 702
Mph1103I	ATGCAT	1 cut(s)	464
MseI	TTAA	2 cut(s)	212, 576
MspI	CCGG	1 cut(s)	704
MspR9I	CCNGG	1 cut(s)	377
Mva1269I	GAATGC	1 cut(s)	455
MvaI	CCWGG	1 cut(s)	377
MwoI	GCNNNNNNNGC	1 cut(s)	459
NdeII	GATC	2 cut(s)	542, 595
NlaIII	CATG	5 cut(s)	71, 259, 466, 506, 637
NlaIV	GGNNCC	1 cut(s)	199
NmuCI	GTSAC	2 cut(s)	337, 566
NsiI	ATGCAT	1 cut(s)	464
NspI	RCATGY	1 cut(s)	71
PaeI	GCATGC	1 cut(s)	71
PctI	GAATGC	1 cut(s)	455
PfeI	GAWTC	2 cut(s)	655, 728
PkrI	GCNGC	1 cut(s)	148
PleI	GAGTC	2 cut(s)	29, 713
PpsI	GAGTC	2 cut(s)	29, 713
Ppu21I	YACGTR	1 cut(s)	675
Psp6I	CCWGG	1 cut(s)	375
PspGI	CCWGG	1 cut(s)	375
PspN4I	GGNNCC	1 cut(s)	199
PsuI	RGATCY	1 cut(s)	542
RsaI	GTAC	1 cut(s)	5
RsaNI	GTAC	1 cut(s)	4
SalI	GTCGAC	1 cut(s)	411
SaqAI	TTAA	2 cut(s)	212, 576
SatI	GCNGC	1 cut(s)	147
Sau3AI	GATC	2 cut(s)	542, 595
SchI	GAGTC	2 cut(s)	29, 713
ScrFI	CCNGG	1 cut(s)	377
SduI	GDGCHC	2 cut(s)	202, 557
SetI	ASST	9 cut(s)	13, 58, 192, 234, 337, 386, 677, 738, 743
SfaNI	GCATC	1 cut(s)	291
SphI	GCATGC	1 cut(s)	71
Sse9I	AATT	4 cut(s)	216, 222, 480, 627
SsiI	CCGC	2 cut(s)	147, 453
StyD4I	CCNGG	1 cut(s)	375
StyI	CCWWGG	2 cut(s)	202, 264
TaaI	ACNGT	4 cut(s)	140, 417, 527, 643
TaiI	ACGT	1 cut(s)	677
TaqI	TCGA	2 cut(s)	412, 429
TasI	AATT	4 cut(s)	216, 222, 480, 627
TatI	WGTACW	1 cut(s)	3
TauI	GCSGC	1 cut(s)	149
TfiI	GAWTC	2 cut(s)	655, 728
Tru1I	TTAA	2 cut(s)	212, 576
Tru9I	TTAA	2 cut(s)	212, 576
TscAI	CASTG	3 cut(s)	375, 422, 648
TseFI	GTSAC	2 cut(s)	337, 566
Tsp45I	GTSAC	2 cut(s)	337, 566
TspGWI	ACGGA	1 cut(s)	665
TspRI	CASTG	3 cut(s)	375, 422, 648
XapI	RAATTY	1 cut(s)	216
XceI	RCATGY	1 cut(s)	71
XmiI	GTMKAC	3 cut(s)	260, 412, 606
Zsp2I	ATGCAT	1 cut(s)	464

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.