MD10G1299900.v1.1
NAC Family

NAC transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010797
Physical Location & Seq
Forward (+)
38727714 .. 38729406
1693 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1299900.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGGAGAGCACAAATTCATCATCAGGTTCACAGCACCCGCAGCTCCCGCCGGGGTTCCGGTTTCACCCGACGGACGAAGAACTGGTGGTCCATTACCTCAAGAAAAAGGCCGCCTCCATCCCGCTTCCGGTCACCATCATCGCGGAGGTCGACTTGTACAAGTTTGATCCATGGGAACTGCCAAGTAAGGCGACGTTTGGGGAGCAAGAGTGGTATTTTTTCAGCCCAAGGGACCGGAAGTATCCAAATGGGGCACGGCCAAACAGGGCAGCAACTTCAGGGTATTGGAAGGCAACTGGGACGGATAAGCCTGTTCTATCATCAATCGGAAACCACAAAGTCGGTGTGAAAAAAGCTCTTGTTTTCTATGGAGGGAAGCCCCCAAAAGGGACTAAAACCAATTGGATTATGCACGAGTATCGGCTTGTCAACAACACCACCACCACCATCAACATGAGTTCTAGTACTACTAAGCCTTCTGATGCAGCTAACAAGAAACCCTCCCTAAGGCTTGATGATTGGGTTTTGTGTCGGATTTATAAGAAAAACAAAGGGCCTGTGATGTCTATAGATGGTGATCATTTTCTTCACAAGGCCGAGGACTCTAATAGTTCTATGGAAGAGGGTATGTTTCTCCACCCGTCAATACTGCCAACTCAAAAACTGCCGCCGCTGCCGCCGCCACCATCATCAAAGCTGCCAACTACAGATTACACCGCGTTGCTAGAAAGCGATGATCACGAGAGCTATCTTGAGGGCATACTATCGTCAAGCGATCATCAACCAGGCTCTCACTCTCATGATCATGATCAGTTTGGCACTATATCAACTTCCATCTCAAAAAGCAGCCTACCCTTGAAACGTCATCTTCCTTCGCCGTTTTGGAACGACGTGGGATCGATAGGGACTGCTGCTGCTACTACTACTTCATCGAGCAAAAAGTTTCATGCTGATCTCATCAATGAAAACAACTCATTTGTTTCTATGCTGAATCAGACGCCACAGGGCACGGCACCGTTTCACGCTAATGCTGCACTTCTTGAGTTGCTAGGAGATGGGGTTTTGCGCCCACAGTTTCAACTCCCAAGCATGAACTGGAATTCCTAG

Protein Analysis

369

Amino Acids

40.66

Weight (kDa)

7.86

Isoelectric Point (pI)

44.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 16 - 142 4.4e-51 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010797)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61110
fragaria_vesca FvH4_3g04630
malus_domestica MD05G1321800.v1.1 MD05G1321900.v1.1 MD10G1299900.v1.1
prunus_persica Prupe.4G040900_v2.0.a1 Prupe.4G040900_v2.0.a1
pyrus_communis pycom05g29620 pycom10g25260
rosa_chinensis RchiOBHm_Chr5g0007561
rosa_laevigata RLG00000031468
rosa_multiflora Rmu_ssc0000182.1_g000018
rosa_roxburghii Rroxscaffold_1G00068840
rosa_rugosa Rorug04G0428600 Rorug04G0428700
rosa_samantha Rh5AG056300 Rh5BG053800 Rh5CG063600 Rh5DG052800
rosa_wichuraiana Rw5G005390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 540
AccB1I GGYRCC 1 cut(s) 1012
AccI GTMKAC 1 cut(s) 150
AccII CGCG 2 cut(s) 143, 719
AclWI GGATC 2 cut(s) 161, 904
AcoI YGGCCR 1 cut(s) 257
AcsI RAATTY 2 cut(s) 13, 1099
AcuI CTGAAG 1 cut(s) 261
AcyI GRCGYC 1 cut(s) 998
AfaI GTAC 2 cut(s) 158, 466
AfiI CCNNNNNNNGG 4 cut(s) 127, 386, 387, 507
AgsI TTSAA 2 cut(s) 859, 1079
AjiI CACGTC 1 cut(s) 892
AjnI CCWGG 1 cut(s) 784
AloI GAACNNNNNNTCC 3 cut(s) 72, 104, 1085
AluBI AGCT 5 cut(s) 43, 356, 488, 697, 747
AluI AGCT 5 cut(s) 43, 356, 488, 697, 747
Alw21I GWGCWC 1 cut(s) 11
AlwI GGATC 2 cut(s) 161, 904
AoxI GGCC 4 cut(s) 108, 257, 554, 594
ApeKI GCWGC 9 cut(s) 40, 269, 485, 673, 697, 846, 911, 914, 1031
ApoI RAATTY 2 cut(s) 13, 1099
AspLEI GCGC 1 cut(s) 1068
AspS9I GGNCC 3 cut(s) 88, 232, 554
AsuC2I CCSGG 1 cut(s) 51
AsuHPI GGTGA 3 cut(s) 56, 124, 587
AvaII GGWCC 2 cut(s) 88, 232
AxyI CCTNAGG 1 cut(s) 506
BaeGI GKGCMC 2 cut(s) 256, 1010
BanI GGYRCC 1 cut(s) 1012
BauI CACGAG 2 cut(s) 413, 740
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 9 cut(s) 52, 281, 497, 660, 684, 858, 898, 901, 1018
BccI CCATC 7 cut(s) 125, 143, 455, 566, 694, 842, 1049
BceAI ACGGC 3 cut(s) 272, 862, 1026
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BciT130I CCWGG 1 cut(s) 786
BciVI GTATCC 1 cut(s) 252
BclI TGATCA 4 cut(s) 577, 736, 802, 808
BcnI CCSGG 1 cut(s) 51
BfaI CTAG 4 cut(s) 462, 725, 1049, 1105
BfmI CTRYAG 2 cut(s) 567, 705
BfuI GTATCC 1 cut(s) 252
BmcAI AGTACT 1 cut(s) 466
Bme1390I CCNGG 2 cut(s) 51, 786
Bme18I GGWCC 2 cut(s) 88, 232
BmgBI CACGTC 1 cut(s) 892
BmgT120I GGNCC 3 cut(s) 88, 232, 554
BmiI GGNNCC 3 cut(s) 56, 233, 1014
BmrFI CCNGG 2 cut(s) 51, 786
BmrI ACTGGG 1 cut(s) 306
BmsI GCATC 1 cut(s) 472
BmuI ACTGGG 1 cut(s) 306
BpuEI CTTGAG 3 cut(s) 83, 773, 1061
BpuMI CCSGG 1 cut(s) 51
Bsa29I ATCGAT 1 cut(s) 899
BsaBI GATNNNNATC 2 cut(s) 576, 807
BsaHI GRCGYC 1 cut(s) 998
BsaJI CCNNGG 4 cut(s) 50, 170, 227, 597
BsaWI WCCGGW 3 cut(s) 57, 127, 234
BsaXI ACNNNNNCTCC 4 cut(s) 194, 224, 1044, 1074
Bsc4I CCNNNNNNNGG 4 cut(s) 127, 386, 387, 507
Bse1I ACTGG 3 cut(s) 87, 301, 1100
Bse21I CCTNAGG 1 cut(s) 506
Bse8I GATNNNNATC 2 cut(s) 576, 807
BseBI CCWGG 1 cut(s) 786
BseCI ATCGAT 1 cut(s) 899
BseDI CCNNGG 4 cut(s) 50, 170, 227, 597
BseGI GGATG 1 cut(s) 117
BseJI GATNNNNATC 2 cut(s) 576, 807
BseLI CCNNNNNNNGG 4 cut(s) 127, 386, 387, 507
BseNI ACTGG 3 cut(s) 87, 301, 1100
BseSI GKGCMC 2 cut(s) 256, 1010
BseXI GCAGC 9 cut(s) 52, 281, 497, 660, 684, 858, 898, 901, 1018
BsgI GTGCAG 1 cut(s) 1017
Bsh1236I CGCG 2 cut(s) 143, 719
BshFI GGCC 4 cut(s) 110, 259, 556, 596
BshNI GGYRCC 1 cut(s) 1012
BshVI ATCGAT 1 cut(s) 899
BsiHKAI GWGCWC 1 cut(s) 11
BsiSI CCGG 4 cut(s) 50, 58, 128, 235
BslFI GGGAC 4 cut(s) 245, 313, 403, 919
BslI CCNNNNNNNGG 4 cut(s) 127, 386, 387, 507
BsmFI GGGAC 4 cut(s) 245, 313, 403, 919
BsnI GGCC 4 cut(s) 110, 259, 556, 596
Bsp1286I GDGCHC 3 cut(s) 11, 256, 1010
Bsp1407I TGTACA 1 cut(s) 156
Bsp143I GATC 8 cut(s) 166, 577, 736, 775, 802, 808, 896, 952
Bsp19I CCATGG 1 cut(s) 170
BspANI GGCC 4 cut(s) 110, 259, 556, 596
BspDI ATCGAT 1 cut(s) 899
BspFNI CGCG 2 cut(s) 143, 719
BspHI TCATGA 2 cut(s) 799, 805
BspLI GGNNCC 3 cut(s) 56, 233, 1014
BspPI GGATC 2 cut(s) 161, 904
BspT107I GGYRCC 1 cut(s) 1012
BsrGI TGTACA 1 cut(s) 156
BsrI ACTGG 3 cut(s) 87, 301, 1100
BssECI CCNNGG 4 cut(s) 50, 170, 227, 597
BssMI GATC 8 cut(s) 166, 577, 736, 775, 802, 808, 896, 952
BssNI GRCGYC 1 cut(s) 998
BssSI CACGAG 2 cut(s) 413, 740
BssT1I CCWWGG 2 cut(s) 170, 227
Bst2BI CACGAG 2 cut(s) 413, 740
Bst2UI CCWGG 1 cut(s) 786
Bst4CI ACNGT 2 cut(s) 1017, 1074
Bst6I CTCTTC 1 cut(s) 615
BstACI GRCGYC 1 cut(s) 998
BstAUI TGTACA 1 cut(s) 156
BstDEI CTNAG 2 cut(s) 471, 506
BstDSI CCRYGG 1 cut(s) 170
BstEII GGTNACC 1 cut(s) 130
BstENI CCTNNNNNAGG 1 cut(s) 505
BstF5I GGATG 1 cut(s) 117
BstFNI CGCG 2 cut(s) 143, 719
BstHHI GCGC 1 cut(s) 1068
BstKTI GATC 8 cut(s) 169, 580, 739, 778, 805, 811, 899, 955
BstMBI GATC 8 cut(s) 166, 577, 736, 775, 802, 808, 896, 952
BstMWI GCNNNNNNNGC 6 cut(s) 40, 46, 673, 676, 679, 1031
BstNI CCWGG 1 cut(s) 786
BstPI GGTNACC 1 cut(s) 130
BstSCI CCNGG 2 cut(s) 49, 784
BstSFI CTRYAG 2 cut(s) 567, 705
BstSLI GKGCMC 2 cut(s) 256, 1010
BstUI CGCG 2 cut(s) 143, 719
BstV1I GCAGC 9 cut(s) 52, 281, 497, 660, 684, 858, 898, 901, 1018
Bsu15I ATCGAT 1 cut(s) 899
Bsu36I CCTNAGG 1 cut(s) 506
BsuI GTATCC 1 cut(s) 252
BsuRI GGCC 4 cut(s) 110, 259, 556, 596
BsuTUI ATCGAT 1 cut(s) 899
BtgI CCRYGG 1 cut(s) 170
BtgZI GCGATG 2 cut(s) 124, 747
BtrI CACGTC 1 cut(s) 892
BtsCI GGATG 1 cut(s) 117
CciI TCATGA 2 cut(s) 799, 805
CfoI GCGC 1 cut(s) 1068
Cfr13I GGNCC 3 cut(s) 88, 232, 554
ClaI ATCGAT 1 cut(s) 899
CseI GACGC 1 cut(s) 1006
Csp6I GTAC 2 cut(s) 157, 465
CviAII CATG 6 cut(s) 171, 454, 800, 806, 947, 1090
CviQI GTAC 2 cut(s) 157, 465
DdeI CTNAG 2 cut(s) 471, 506
DpnI GATC 8 cut(s) 168, 579, 738, 777, 804, 810, 898, 954
DpnII GATC 8 cut(s) 166, 577, 736, 775, 802, 808, 896, 952
EaeI YGGCCR 1 cut(s) 257
Eam1104I CTCTTC 1 cut(s) 615
EarI CTCTTC 1 cut(s) 615
Eco130I CCWWGG 2 cut(s) 170, 227
Eco47I GGWCC 2 cut(s) 88, 232
Eco57I CTGAAG 1 cut(s) 261
Eco81I CCTNAGG 1 cut(s) 506
Eco91I GGTNACC 1 cut(s) 130
EcoNI CCTNNNNNAGG 1 cut(s) 505
EcoO109I RGGNCCY 1 cut(s) 554
EcoO65I GGTNACC 1 cut(s) 130
EcoRI GAATTC 1 cut(s) 1099
EcoRII CCWGG 1 cut(s) 784
EcoT14I CCWWGG 2 cut(s) 170, 227
ErhI CCWWGG 2 cut(s) 170, 227
FaeI CATG 6 cut(s) 174, 457, 803, 809, 950, 1093
FaqI GGGAC 4 cut(s) 245, 313, 403, 919
FatI CATG 6 cut(s) 170, 453, 799, 805, 946, 1089
FauI CCCGC 3 cut(s) 45, 54, 129
FbaI TGATCA 4 cut(s) 577, 736, 802, 808
FblI GTMKAC 1 cut(s) 150
FokI GGATG 1 cut(s) 104
FspBI CTAG 4 cut(s) 462, 725, 1049, 1105
GlaI GCGC 1 cut(s) 1067
HaeIII GGCC 4 cut(s) 110, 259, 556, 596
HapII CCGG 4 cut(s) 50, 58, 128, 235
HgaI GACGC 1 cut(s) 1006
HhaI GCGC 1 cut(s) 1068
Hin1I GRCGYC 1 cut(s) 998
Hin1II CATG 6 cut(s) 174, 457, 803, 809, 950, 1093
Hin6I GCGC 1 cut(s) 1066
HinP1I GCGC 1 cut(s) 1066
HincII GTYRAC 2 cut(s) 151, 430
HindII GTYRAC 2 cut(s) 151, 430
HinfI GANTC 2 cut(s) 602, 991
HpaII CCGG 4 cut(s) 50, 58, 128, 235
HphI GGTGA 3 cut(s) 56, 124, 587
Hpy166II GTNNAC 3 cut(s) 29, 151, 430
Hpy188I TCNGA 4 cut(s) 329, 481, 534, 996
Hpy188III TCNNGA 6 cut(s) 100, 740, 752, 800, 806, 1040
Hpy8I GTNNAC 3 cut(s) 29, 151, 430
Hpy99I CGWCG 3 cut(s) 73, 196, 893
HpyAV CCTTC 3 cut(s) 283, 486, 882
HpyCH4III ACNGT 2 cut(s) 1017, 1074
HpyCH4IV ACGT 3 cut(s) 194, 862, 891
HpyCH4V TGCA 3 cut(s) 412, 485, 1034
HpyF10VI GCNNNNNNNGC 6 cut(s) 40, 46, 673, 676, 679, 1031
HpyF3I CTNAG 2 cut(s) 471, 506
HpySE526I ACGT 3 cut(s) 194, 862, 891
Hsp92I GRCGYC 1 cut(s) 998
Hsp92II CATG 6 cut(s) 174, 457, 803, 809, 950, 1093
HspAI GCGC 1 cut(s) 1066
Ksp22I TGATCA 4 cut(s) 577, 736, 802, 808
Kzo9I GATC 8 cut(s) 166, 577, 736, 775, 802, 808, 896, 952
LmnI GCTCC 2 cut(s) 48, 202
Lsp1109I GCAGC 9 cut(s) 52, 281, 497, 660, 684, 858, 898, 901, 1018
LweI GCATC 1 cut(s) 472
MaeI CTAG 4 cut(s) 462, 725, 1049, 1105
MaeII ACGT 3 cut(s) 194, 862, 891
MaeIII GTNAC 1 cut(s) 130
MalI GATC 8 cut(s) 168, 579, 738, 777, 804, 810, 898, 954
MboI GATC 8 cut(s) 166, 577, 736, 775, 802, 808, 896, 952
MboII GAAGA 4 cut(s) 89, 578, 632, 860
MfeI CAATTG 1 cut(s) 400
MhlI GDGCHC 3 cut(s) 11, 256, 1010
MluCI AATT 3 cut(s) 13, 400, 1099
MlyI GAGTC 1 cut(s) 596
MmeI TCCRAC 1 cut(s) 512
MnlI CCTC 8 cut(s) 107, 124, 139, 365, 511, 592, 616, 748
MslI CAYNNNNRTG 4 cut(s) 452, 798, 804, 1026
MspA1I CMGCKG 1 cut(s) 673
MspI CCGG 4 cut(s) 50, 58, 128, 235
MspR9I CCNGG 2 cut(s) 51, 786
MunI CAATTG 1 cut(s) 400
MvaI CCWGG 1 cut(s) 786
MvnI CGCG 2 cut(s) 143, 719
MwoI GCNNNNNNNGC 6 cut(s) 40, 46, 673, 676, 679, 1031
NciI CCSGG 1 cut(s) 51
NcoI CCATGG 1 cut(s) 170
NdeII GATC 8 cut(s) 166, 577, 736, 775, 802, 808, 896, 952
NlaIII CATG 6 cut(s) 174, 457, 803, 809, 950, 1093
NlaIV GGNNCC 3 cut(s) 56, 233, 1014
NmeAIII GCCGAG 1 cut(s) 622
NmuCI GTSAC 1 cut(s) 130
PagI TCATGA 2 cut(s) 799, 805
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PfeI GAWTC 1 cut(s) 991
PleI GAGTC 1 cut(s) 596
PpsI GAGTC 1 cut(s) 596
PsiI TTATAA 1 cut(s) 540
Psp6I CCWGG 1 cut(s) 784
PspEI GGTNACC 1 cut(s) 130
PspGI CCWGG 1 cut(s) 784
PspN4I GGNNCC 3 cut(s) 56, 233, 1014
PspPI GGNCC 3 cut(s) 88, 232, 554
RsaI GTAC 2 cut(s) 158, 466
RsaNI GTAC 2 cut(s) 157, 465
RseI CAYNNNNRTG 4 cut(s) 452, 798, 804, 1026
SalI GTCGAC 1 cut(s) 149
Sau3AI GATC 8 cut(s) 166, 577, 736, 775, 802, 808, 896, 952
Sau96I GGNCC 3 cut(s) 88, 232, 554
ScaI AGTACT 1 cut(s) 466
SchI GAGTC 1 cut(s) 596
ScrFI CCNGG 2 cut(s) 51, 786
SduI GDGCHC 3 cut(s) 11, 256, 1010
SfaNI GCATC 1 cut(s) 472
SfcI CTRYAG 2 cut(s) 567, 705
SinI GGWCC 2 cut(s) 88, 232
SmiMI CAYNNNNRTG 4 cut(s) 452, 798, 804, 1026
SmlI CTYRAG 3 cut(s) 98, 752, 1040
SmoI CTYRAG 3 cut(s) 98, 752, 1040
Sse9I AATT 3 cut(s) 13, 400, 1099
SspMI CTAG 4 cut(s) 462, 725, 1049, 1105
StyD4I CCNGG 2 cut(s) 49, 784
StyI CCWWGG 2 cut(s) 170, 227
TaaI ACNGT 2 cut(s) 1017, 1074
TaiI ACGT 3 cut(s) 197, 865, 894
TaqI TCGA 3 cut(s) 150, 899, 932
TasI AATT 3 cut(s) 13, 400, 1099
TatI WGTACW 2 cut(s) 156, 464
TauI GCSGC 5 cut(s) 113, 670, 673, 679, 682
TfiI GAWTC 1 cut(s) 991
TseFI GTSAC 1 cut(s) 130
TseI GCWGC 9 cut(s) 40, 269, 485, 673, 697, 846, 911, 914, 1031
Tsp45I GTSAC 1 cut(s) 130
TspDTI ATGAA 5 cut(s) 6, 918, 935, 978, 1106
TspGWI ACGGA 2 cut(s) 86, 317
VpaK11BI GGWCC 2 cut(s) 88, 232
XagI CCTNNNNNAGG 1 cut(s) 505
XapI RAATTY 2 cut(s) 13, 1099
XcmI CCANNNNNNNNNTGG 1 cut(s) 1092
XmiI GTMKAC 1 cut(s) 150
XspI CTAG 4 cut(s) 462, 725, 1049, 1105
ZrmI AGTACT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.