Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0009992

Physical Location & Seq

Genomic Coordinates Reverse (-)

39943714 .. 39950130

Gene Structure 6417 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1315900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2931 bp

ATGGACTCGGAAAGGCTCACACTTTCCCGAGAAAACCCTAGAGTCGCTTTTTCCCCGCACACCGCCGGAAAGGCCATGCCCGGTGCCGAGTTAGCTCAGAAGCCCCAGCCTCCGACTCCGATAGTGGACCGATTCAGGGCTCTGCTCAAGCAGCGGGAGGAGGACCTCAGGGTTTCGCCCGACGACGAGGTATCGCCGCCCAGCACCGAAGAAATCGTCCACCTTTACGAGATGGTATTGTCGGAGCTCATCTTTAATTCGAAGCCCATCATTACCGATCTCACCATCATCGCCGGCGAGCAGAGAGACCACGGAAGGGGCATCGCCAACGCCATCTGCGCCCGCATTCTCGAGGTCCCGGTCGAGCATAAACTGCCTTCACTGTATCTTTTAGACAGTATTGTCAAGAATATTGGCCGAGAATATGCCAAGTTTTTCTCTTCCCGTCTTCCTGAGGTCTTTTGCGAGGCATACAGGCAAGTACACCCTAACCAGCACCCTGCCATGCGCCACCTCTTTGGCACTTGGTCAGCAGTGTTTCCACCTTCAGTCCTTCGCAGGATTGAAGAGCAACTGCAATTTTCTCCGCAAGCAAACCAACAATCATCAGGATTACCACCTTTGAGGACTTCTGAATCTCCTCGACCAGCTCATGGCATCCATGTCAATCCAAAATATTTACGTCCGTTGGACAGTTCAGATGTAGATGGTGTTGTATCAAAAAGATTAAACTCAACAGGAAGTGTGAGTCATTCACCTTTTGCCCTGGGGTCCAATCAATTACACCCATCTTCAACTGCTAGGCTTGCAAGATCTTCGTCGCCTTCTAATATTGGGCTTGATAGGTCTTTGCCATCAGCAGTTGATGAATATGCAGCAGAACAGTCTCCAAGAAGGTTTGTTGAGAGGGCTTCTCCATCTAATTCCGTGTTTGATTATAGACTTGGTGGAGCGATGGGTAAGGATGAGGAGTCGAATGAATGGCGGAGAAAACGCTATCTTGATGGTAGTCAGAAGTGGTTTGATACTTCTGCTGCATATAATGGAGTTGACCATCAGAACCCAAGAGCTTTAATTGATGCATATGGGAAGGACAGTGCAGATAGAAGTTTAAATGATAAGCCTCTGCTGGTTGGGCAGCTGGGCCTGAACGGTCTAGATCATAAGGCAACTCCAATGTCATGGCAGAATACTGAAGAGGAAGAGTTTGATTGGGAAGATGTGACCCCAAATTTAGCAGACCATGGTCGGGGTAACGATTTCTTGGCTTCAACTGTACCGCCTTCTAGAAGCTACAGGGCAAGCCATGGAACACAGAATGCAAGCTCCTTGGAGCCAGATGTTAGGAGTACTTGGTCTAGTCAGGCACATCCACCTTCAGCCGAACAGTCCTCTATTATAGCTGAAGATTCAGTTCCCTCACTTGGTTTCGGTCGTGGATCATCGGGTGCTGTGTCTCGATTTCAGTCTGAGAAAAATCATAATATGGGTTCTCGCTACCCTCAAGAAGCCTGGAATATGCCTTTCCATCACTTGCAGCCTTTGCAGAATCCCCTTAATGCCAAAGGAAGAGGAGGGAACATTCAGACGCCCTTTGTGACAAGTGGTATATCTTCAGGTGGTGAGAAAATGTCTGCCTTTAATGACAAGCTTCTAGATGTTGATACACGACTTCATGGGCCCATTACATCAAGAATGGGTTCCTCTAGTGTTGACTCTGGTACTGCTGACTCTCGATCTATGATGCCAGTGTCAATGGGTTTAAGGCCTCCAGTAAACGCGCAAAATTCTCACCCTCCCCGAGTGCACTCAGTTTTTGCAATGCAGAACCAGAGGAATCCGTACGGTTCAATAAATTATAGCAATACTGTAAAAAATCAAGGCCCATATAATCCTTCATACATGCCGGAGCAGCAGTTAGATGGCTATGAAAACAAGGAGTCAAGGTCGACAAAGTCAACTCAGGTGCCACAATATCGTCCCTTACAACCACAGTATCGTCCACCTCAGGAGGCACGGGAGAATATTTTTTCGTCTGCAGAAACTCAGGTGCCATCTTATTTAGGGGTACCACCCTTAAATCATGGATACACTTTGCGAGGTCCCGTACCTCGTCAACACTTGGGCATACCAACCCCCTATAACCCAAATGGCAGTTCTCAGTTCTCCTTGCCACCTCTACCACCAGGTCCTCCTCCTCCCTCGCAAGGCATACTTTCCGTCCAAAACCCTGGTTCAGTTGTCTCTAGCAACCAGCCCGGCAATTCCTATTCTGGTTTGATTAGTTCTCTCATGGCTCAAGGTTTGATCTCATTGACAAAGCAAAGCCCTGTCCAGGATTCTGTAGGAGTCGAGTTTAATGCCGACCTTCTCAAGGTGCGTCATGAATCTGTAATAAGTGCCCTATATGGTGATCTCCCTAGACAATGCACAACCTGTGGCCTTAGGTTCAAGTGCCAAGAAGAGCACAGTAGTCATATGGATTGGCATGTAACAAAGAATCGTATGTCTAAAAACCGCAAGCAGAAGCCCTCTCGCAAATGGTTTGTAAATACAAGCATGTGGCTCACTGGTGCAGAGGCATTGGGTACTGAGGCAGTCCCTGGATTTTTGCCTGCCGAGACCATAGTGGAAAAGAAAAGTGATGAAGAAATGGCTGTTCCTGCCGATGAGGATCAGAATTCGTGTGCATTATGTGGAGAGACTTTTGATGACTTTTATAGTGATGAGAATGAGGAGTGGATGTATAAAGATGCTGTGTACATGAATGCACCTGACGGAGCAACAGGAGGCATGGATAGGTCACAATTAGGTCCTATTGTGCATGCTAAATGCAGATCCGAGTCAAGTGTGGTCTCCCCTGAAGGTTTTGGACAAGATCAAAGGGGAATTATTGAAGAGGGTAGTCAAAGAAAACGATTGCGGAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000993 GO:0001098 GO:0001099 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005849 GO:0006139 GO:0006351 GO:0006353 GO:0006366 GO:0006369 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009909 GO:0009911 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0018130 GO:0019438 GO:0019899 GO:0031123 GO:0031124 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0046483 GO:0048518 GO:0048580 GO:0048582 GO:0048831 GO:0050789 GO:0050793 GO:0051094 GO:0051239 GO:0051240 GO:0065007 GO:0070013 GO:0070063 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

CTD_bind

Protein Analysis

977

Amino Acids

107.68

Weight (kDa)

6.26

Isoelectric Point (pI)

61.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CID	PF04818	80 - 185	1.9e-11	CID domain
zf_PCFS4	PF23228	882 - 948	9.5e-30	PCFS4 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009992)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04885
fragaria_vesca	FvH4_3g02510 FvH4_3g02510 FvH4_3g02510 FvH4_3g02510
malus_domestica	MD05G1342300.v1.1 MD10G1315900.v1.1
prunus_persica	Prupe.4G024700_v2.0.a1
pyrus_communis	pycom05g31160 pycom10g26810
rosa_chinensis	RchiOBHm_Chr5g0003821
rosa_laevigata	RLG00000031178
rosa_multiflora	Rmu_sc0004212.1_g000001
rosa_roxburghii	Rroxscaffold_1G00072170
rosa_rugosa	Rorug04G0406900 Rorug04G0407000
rosa_samantha	Rh5AG034400 Rh5BG032700 Rh5CG036800 Rh5DG033100
rosa_wichuraiana	Rw5G003150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	401
Acc65I	GGTACC	1 cut(s)	2068
AccB1I	GGYRCC	4 cut(s)	83, 1966, 2050, 2068
AccB7I	CCANNNNNTGG	1 cut(s)	653
AccI	GTMKAC	1 cut(s)	1949
AccII	CGCG	1 cut(s)	1781
AclWI	GGATC	3 cut(s)	1447, 2682, 2832
AcoI	YGGCCR	1 cut(s)	415
AcsI	RAATTY	3 cut(s)	1231, 1786, 2680
AcuI	CTGAAG	6 cut(s)	531, 1215, 1362, 1425, 1599, 2883
AcyI	GRCGYC	1 cut(s)	1589
AfaI	GTAC	9 cut(s)	483, 1278, 1351, 1723, 1844, 2070, 2109, 2590, 2762
AfiI	CCNNNNNNNGG	8 cut(s)	136, 316, 653, 1249, 1424, 2207, 2335, 2374
AgsI	TTSAA	6 cut(s)	566, 795, 1272, 1851, 2452, 2897
AjnI	CCWGG	6 cut(s)	765, 1511, 2185, 2230, 2334, 2602
AjuI	GAANNNNNNNTTGG	2 cut(s)	2140, 2172
AluBI	AGCT	9 cut(s)	95, 247, 650, 1070, 1141, 1293, 1326, 1403, 1651
AluI	AGCT	9 cut(s)	95, 247, 650, 1070, 1141, 1293, 1326, 1403, 1651
Alw21I	GWGCWC	3 cut(s)	249, 1809, 2469
Alw26I	GTCTC	7 cut(s)	300, 891, 1461, 2248, 2615, 2696, 2860
Alw44I	GTGCAC	1 cut(s)	1805
AlwI	GGATC	3 cut(s)	1447, 2682, 2832
AlwNI	CAGNNNCTG	1 cut(s)	2603
Ama87I	CYCGRG	3 cut(s)	27, 350, 1800
AoxI	GGCC	7 cut(s)	72, 415, 1144, 1679, 1766, 1882, 2440
ApaI	GGGCCC	1 cut(s)	1683
ApaLI	GTGCAC	1 cut(s)	1805
ApeKI	GCWGC	6 cut(s)	151, 875, 1034, 1138, 1537, 1912
ApoI	RAATTY	3 cut(s)	1231, 1786, 2680
ArsI	GACNNNNNNTTYG	2 cut(s)	201, 233
Asp700I	GAANNNNTTC	1 cut(s)	1699
Asp718I	GGTACC	1 cut(s)	2068
AspLEI	GCGC	3 cut(s)	341, 510, 1783
AsuC2I	CCSGG	3 cut(s)	81, 359, 2259
AsuHPI	GGTGA	5 cut(s)	274, 747, 1634, 1784, 2423
AsuII	TTCGAA	1 cut(s)	260
AvaI	CYCGRG	3 cut(s)	27, 350, 1800
AvaII	GGWCC	7 cut(s)	127, 163, 355, 771, 2102, 2189, 2813
AxyI	CCTNAGG	4 cut(s)	167, 453, 2007, 2444
BaeGI	GKGCMC	3 cut(s)	1683, 1809, 2404
BaeI	ACNNNNGTAYC	2 cut(s)	1979, 2012
BanI	GGYRCC	4 cut(s)	83, 1966, 2050, 2068
BanII	GRGCYC	3 cut(s)	142, 249, 1683
BbsI	GAAGAC	1 cut(s)	440
Bbv12I	GWGCWC	3 cut(s)	249, 1809, 2469
BbvI	GCAGC	6 cut(s)	163, 887, 1021, 1150, 1549, 1924
BcgI	CGANNNNNNTGC	4 cut(s)	798, 832, 2342, 2376
BciT130I	CCWGG	6 cut(s)	767, 1513, 2187, 2232, 2336, 2604
BciVI	GTATCC	1 cut(s)	2081
BcnI	CCSGG	3 cut(s)	81, 359, 2259
BcoDI	GTCTC	7 cut(s)	300, 891, 1461, 2248, 2615, 2696, 2860
BfaI	CTAG	9 cut(s)	39, 801, 1157, 1287, 1359, 1655, 1707, 2247, 2421
BfmI	CTRYAG	3 cut(s)	1294, 2037, 2343
BfuI	GTATCC	1 cut(s)	2081
BglI	GCCNNNNNGGC	1 cut(s)	71
BglII	AGATCT	1 cut(s)	812
BisI	GCNGC	7 cut(s)	152, 197, 876, 1035, 1139, 1538, 1913
BlsI	GCNGC	7 cut(s)	153, 198, 877, 1036, 1140, 1539, 1914
BmcAI	AGTACT	1 cut(s)	1351
Bme1390I	CCNGG	9 cut(s)	81, 359, 767, 1513, 2187, 2232, 2259, 2336, 2604
Bme18I	GGWCC	7 cut(s)	127, 163, 355, 771, 2102, 2189, 2813
BmeT110I	CYCGRG	3 cut(s)	27, 350, 1800
BmrFI	CCNGG	9 cut(s)	81, 359, 767, 1513, 2187, 2232, 2259, 2336, 2604
BmsI	GCATC	5 cut(s)	330, 666, 1069, 1734, 2743
BoxI	GACNNNNGTC	1 cut(s)	1245
BpiI	GAAGAC	1 cut(s)	440
BplI	GAGNNNNNCTC	2 cut(s)	898, 930
BpmI	CTGGAG	1 cut(s)	1755
Bpu14I	TTCGAA	1 cut(s)	260
BpuEI	CTTGAG	4 cut(s)	131, 1488, 2283, 2357
BpuMI	CCSGG	3 cut(s)	81, 359, 2259
BsaBI	GATNNNNATC	1 cut(s)	2673
BsaHI	GRCGYC	1 cut(s)	1589
BsaI	GGTCTC	3 cut(s)	300, 2615, 2860
BsaJI	CCNNGG	8 cut(s)	310, 765, 766, 1243, 1306, 1329, 2230, 2602
BsaXI	ACNNNNNCTCC	6 cut(s)	1901, 1931, 1931, 1961, 2729, 2759
Bsc4I	CCNNNNNNNGG	8 cut(s)	136, 316, 653, 1249, 1424, 2207, 2335, 2374
Bse118I	RCCGGY	1 cut(s)	293
Bse1I	ACTGG	3 cut(s)	1748, 1772, 2575
Bse21I	CCTNAGG	4 cut(s)	167, 453, 2007, 2444
Bse3DI	GCAATG	1 cut(s)	1827
Bse8I	GATNNNNATC	1 cut(s)	2673
BseBI	CCWGG	6 cut(s)	767, 1513, 2187, 2232, 2336, 2604
BseDI	CCNNGG	8 cut(s)	310, 765, 766, 1243, 1306, 1329, 2230, 2602
BseGI	GGATG	4 cut(s)	657, 970, 1369, 2748
BseJI	GATNNNNATC	1 cut(s)	2673
BseLI	CCNNNNNNNGG	8 cut(s)	136, 316, 653, 1249, 1424, 2207, 2335, 2374
BseMI	GCAATG	1 cut(s)	1827
BseNI	ACTGG	3 cut(s)	1748, 1772, 2575
BseRI	GAGGAG	7 cut(s)	173, 630, 983, 1587, 2184, 2187, 2750
BseSI	GKGCMC	3 cut(s)	1683, 1809, 2404
BseXI	GCAGC	6 cut(s)	163, 887, 1021, 1150, 1549, 1924
BseYI	CCCAGC	3 cut(s)	105, 200, 1141
BsgI	GTGCAG	2 cut(s)	1119, 2595
Bsh1236I	CGCG	1 cut(s)	1781
Bsh1285I	CGRYCG	2 cut(s)	363, 1435
BshFI	GGCC	7 cut(s)	74, 417, 1146, 1681, 1768, 1884, 2442
BshNI	GGYRCC	4 cut(s)	83, 1966, 2050, 2068
BsiEI	CGRYCG	2 cut(s)	363, 1435
BsiHKAI	GWGCWC	3 cut(s)	249, 1809, 2469
BsiHKCI	CYCGRG	3 cut(s)	27, 350, 1800
BsiSI	CCGG	6 cut(s)	66, 81, 294, 359, 1907, 2259
BsiWI	CGTACG	1 cut(s)	1842
BslFI	GGGAC	4 cut(s)	341, 1965, 2088, 2585
BslI	CCNNNNNNNGG	8 cut(s)	136, 316, 653, 1249, 1424, 2207, 2335, 2374
BsmAI	GTCTC	7 cut(s)	300, 891, 1461, 2248, 2615, 2696, 2860
BsmFI	GGGAC	4 cut(s)	341, 1965, 2088, 2585
BsmI	GAATGC	3 cut(s)	345, 1324, 2773
BsnI	GGCC	7 cut(s)	74, 417, 1146, 1681, 1768, 1884, 2442
Bso31I	GGTCTC	3 cut(s)	300, 2615, 2860
BsoBI	CYCGRG	3 cut(s)	27, 350, 1800
Bsp119I	TTCGAA	1 cut(s)	260
Bsp120I	GGGCCC	1 cut(s)	1679
Bsp1286I	GDGCHC	6 cut(s)	142, 249, 1683, 1809, 2404, 2469
Bsp1407I	TGTACA	1 cut(s)	2760
Bsp19I	CCATGG	2 cut(s)	1243, 1306
BspANI	GGCC	7 cut(s)	74, 417, 1146, 1681, 1768, 1884, 2442
BspFNI	CGCG	1 cut(s)	1781
BspHI	TCATGA	1 cut(s)	2383
BspMAI	CTGCAG	1 cut(s)	2041
BspPI	GGATC	3 cut(s)	1447, 2682, 2832
BspQI	GCTCTTC	2 cut(s)	561, 2457
BspT104I	TTCGAA	1 cut(s)	260
BspT107I	GGYRCC	4 cut(s)	83, 1966, 2050, 2068
BspTNI	GGTCTC	3 cut(s)	300, 2615, 2860
BsrDI	GCAATG	1 cut(s)	1827
BsrFI	RCCGGY	1 cut(s)	293
BsrGI	TGTACA	1 cut(s)	2760
BsrI	ACTGG	3 cut(s)	1748, 1772, 2575
BssAI	RCCGGY	1 cut(s)	293
BssECI	CCNNGG	8 cut(s)	310, 765, 766, 1243, 1306, 1329, 2230, 2602
BssNI	GRCGYC	1 cut(s)	1589
BssT1I	CCWWGG	3 cut(s)	1243, 1306, 1329
Bst2UI	CCWGG	6 cut(s)	767, 1513, 2187, 2232, 2336, 2604
Bst6I	CTCTTC	7 cut(s)	445, 561, 1191, 1197, 1564, 2457, 2892
BstACI	GRCGYC	1 cut(s)	1589
BstAPI	GCANNNNNTGC	2 cut(s)	373, 1543
BstAUI	TGTACA	1 cut(s)	2760
BstBI	TTCGAA	1 cut(s)	260
BstDSI	CCRYGG	3 cut(s)	310, 1243, 1306
BstENI	CCTNNNNNAGG	1 cut(s)	2372
BstF5I	GGATG	4 cut(s)	657, 970, 1369, 2748
BstFNI	CGCG	1 cut(s)	1781
BstHHI	GCGC	3 cut(s)	341, 510, 1783
BstMAI	GTCTC	7 cut(s)	300, 891, 1461, 2248, 2615, 2696, 2860
BstMCI	CGRYCG	2 cut(s)	363, 1435
BstNI	CCWGG	6 cut(s)	767, 1513, 2187, 2232, 2336, 2604
BstNSI	RCATGY	4 cut(s)	1906, 2492, 2563, 2828
BstPAI	GACNNNNGTC	1 cut(s)	1245
BstSCI	CCNGG	9 cut(s)	79, 357, 765, 1511, 2185, 2230, 2257, 2334, 2602
BstSFI	CTRYAG	3 cut(s)	1294, 2037, 2343
BstSLI	GKGCMC	3 cut(s)	1683, 1809, 2404
BstUI	CGCG	1 cut(s)	1781
BstV1I	GCAGC	6 cut(s)	163, 887, 1021, 1150, 1549, 1924
BstV2I	GAAGAC	1 cut(s)	440
BstX2I	RGATCY	2 cut(s)	812, 2837
BstXI	CCANNNNNNTGG	3 cut(s)	518, 1182, 2231
BstYI	RGATCY	2 cut(s)	812, 2837
Bsu36I	CCTNAGG	4 cut(s)	167, 453, 2007, 2444
BsuI	GTATCC	1 cut(s)	2081
BsuRI	GGCC	7 cut(s)	74, 417, 1146, 1681, 1768, 1884, 2442
BtgI	CCRYGG	3 cut(s)	310, 1243, 1306
BtgZI	GCGATG	3 cut(s)	274, 307, 968
BtsCI	GGATG	4 cut(s)	657, 970, 1369, 2748
BtsI	GCAGTG	1 cut(s)	540
BtsIMutI	CAGTG	5 cut(s)	380, 540, 1102, 1755, 2568
CaiI	CAGNNNCTG	1 cut(s)	2603
CciI	TCATGA	1 cut(s)	2383
CfoI	GCGC	3 cut(s)	341, 510, 1783
Cfr10I	RCCGGY	1 cut(s)	293
CseI	GACGC	2 cut(s)	1597, 2369
CsiI	ACCWGGT	1 cut(s)	2185
Csp6I	GTAC	9 cut(s)	482, 1277, 1350, 1722, 1843, 2069, 2108, 2589, 2761
CviQI	GTAC	9 cut(s)	482, 1277, 1350, 1722, 1843, 2069, 2108, 2589, 2761
DraI	TTTAAA	1 cut(s)	1113
DrdI	GACNNNNNNGTC	1 cut(s)	401
DseDI	GACNNNNNNGTC	1 cut(s)	401
EaeI	YGGCCR	1 cut(s)	415
Eam1104I	CTCTTC	7 cut(s)	445, 561, 1191, 1197, 1564, 2457, 2892
EarI	CTCTTC	7 cut(s)	445, 561, 1191, 1197, 1564, 2457, 2892
EciI	GGCGGA	1 cut(s)	1000
Ecl136II	GAGCTC	1 cut(s)	247
Eco130I	CCWWGG	3 cut(s)	1243, 1306, 1329
Eco147I	AGGCCT	1 cut(s)	1768
Eco24I	GRGCYC	3 cut(s)	142, 249, 1683
Eco31I	GGTCTC	3 cut(s)	300, 2615, 2860
Eco47I	GGWCC	7 cut(s)	127, 163, 355, 771, 2102, 2189, 2813
Eco53kI	GAGCTC	1 cut(s)	247
Eco57I	CTGAAG	6 cut(s)	531, 1215, 1362, 1425, 1599, 2883
Eco81I	CCTNAGG	4 cut(s)	167, 453, 2007, 2444
Eco88I	CYCGRG	3 cut(s)	27, 350, 1800
EcoICRI	GAGCTC	1 cut(s)	247
EcoNI	CCTNNNNNAGG	1 cut(s)	2372
EcoO109I	RGGNCCY	5 cut(s)	163, 355, 2102, 2189, 2813
EcoRI	GAATTC	1 cut(s)	2680
EcoRII	CCWGG	6 cut(s)	765, 1511, 2185, 2230, 2334, 2602
EcoT14I	CCWWGG	3 cut(s)	1243, 1306, 1329
EcoT22I	ATGCAT	1 cut(s)	1084
EcoT38I	GRGCYC	3 cut(s)	142, 249, 1683
ErhI	CCWWGG	3 cut(s)	1243, 1306, 1329
FalI	AAGNNNNNCTT	2 cut(s)	2199, 2231
FaqI	GGGAC	4 cut(s)	341, 1965, 2088, 2585
FauI	CCCGC	3 cut(s)	63, 147, 350
FauNDI	CATATG	2 cut(s)	1084, 2478
FblI	GTMKAC	1 cut(s)	1949
Fnu4HI	GCNGC	7 cut(s)	152, 197, 876, 1035, 1139, 1538, 1913
FokI	GGATG	4 cut(s)	644, 977, 1356, 2755
FriOI	GRGCYC	3 cut(s)	142, 249, 1683
Fsp4HI	GCNGC	7 cut(s)	152, 197, 876, 1035, 1139, 1538, 1913
FspBI	CTAG	9 cut(s)	39, 801, 1157, 1287, 1359, 1655, 1707, 2247, 2421
GlaI	GCGC	3 cut(s)	340, 509, 1782
GluI	GCNGC	7 cut(s)	152, 197, 876, 1035, 1139, 1538, 1913
GsaI	CCCAGC	3 cut(s)	109, 204, 1145
GsuI	CTGGAG	1 cut(s)	1755
HaeIII	GGCC	7 cut(s)	74, 417, 1146, 1681, 1768, 1884, 2442
HapII	CCGG	6 cut(s)	66, 81, 294, 359, 1907, 2259
HgaI	GACGC	2 cut(s)	1597, 2369
HhaI	GCGC	3 cut(s)	341, 510, 1783
Hin1I	GRCGYC	1 cut(s)	1589
Hin6I	GCGC	3 cut(s)	339, 508, 1781
HinP1I	GCGC	3 cut(s)	339, 508, 1781
HincII	GTYRAC	5 cut(s)	1051, 1714, 1950, 1959, 2117
HindII	GTYRAC	5 cut(s)	1051, 1714, 1950, 1959, 2117
HindIII	AAGCTT	1 cut(s)	1649
HpaII	CCGG	6 cut(s)	66, 81, 294, 359, 1907, 2259
HphI	GGTGA	5 cut(s)	274, 747, 1634, 1784, 2423
Hpy99I	CGWCG	3 cut(s)	185, 188, 823
HpyCH4IV	ACGT	1 cut(s)	682
HpySE526I	ACGT	1 cut(s)	682
Hsp92I	GRCGYC	1 cut(s)	1589
HspAI	GCGC	3 cut(s)	339, 508, 1781
KpnI	GGTACC	1 cut(s)	2072
KroI	GCCGGC	1 cut(s)	293
KroNI	GCCGGC	1 cut(s)	295
LguI	GCTCTTC	2 cut(s)	561, 2457
LmnI	GCTCC	6 cut(s)	244, 950, 1331, 1333, 1909, 2780
Lsp1109I	GCAGC	6 cut(s)	163, 887, 1021, 1150, 1549, 1924
LweI	GCATC	5 cut(s)	330, 666, 1069, 1734, 2743
MabI	ACCWGGT	1 cut(s)	2185
MaeI	CTAG	9 cut(s)	39, 801, 1157, 1287, 1359, 1655, 1707, 2247, 2421
MaeII	ACGT	1 cut(s)	682
MaeIII	GTNAC	5 cut(s)	1222, 1253, 1597, 2491, 2802
MflI	RGATCY	2 cut(s)	812, 2837
MhlI	GDGCHC	6 cut(s)	142, 249, 1683, 1809, 2404, 2469
MlyI	GAGTC	9 cut(s)	51, 109, 757, 980, 1709, 1724, 1949, 2358, 2852
MmeI	TCCRAC	3 cut(s)	137, 222, 669
Mph1103I	ATGCAT	1 cut(s)	1084
MreI	CGCCGGCG	1 cut(s)	293
MroNI	GCCGGC	1 cut(s)	293
MroXI	GAANNNNTTC	1 cut(s)	1699
MspA1I	CMGCKG	2 cut(s)	154, 1141
MspI	CCGG	6 cut(s)	66, 81, 294, 359, 1907, 2259
MspR9I	CCNGG	9 cut(s)	81, 359, 767, 1513, 2187, 2232, 2259, 2336, 2604
Mva1269I	GAATGC	3 cut(s)	345, 1324, 2773
MvaI	CCWGG	6 cut(s)	767, 1513, 2187, 2232, 2336, 2604
MvnI	CGCG	1 cut(s)	1781
NaeI	GCCGGC	1 cut(s)	295
NciI	CCSGG	3 cut(s)	81, 359, 2259
NcoI	CCATGG	2 cut(s)	1243, 1306
NdeI	CATATG	2 cut(s)	1084, 2478
NgoMIV	GCCGGC	1 cut(s)	293
NmeAIII	GCCGAG	3 cut(s)	112, 443, 2644
NmuCI	GTSAC	3 cut(s)	1222, 1597, 2802
NsiI	ATGCAT	1 cut(s)	1084
NspI	RCATGY	4 cut(s)	1906, 2492, 2563, 2828
NspV	TTCGAA	1 cut(s)	260
PaeI	GCATGC	1 cut(s)	2828
PaeR7I	CTCGAG	1 cut(s)	350
PagI	TCATGA	1 cut(s)	2383
PasI	CCCWGGG	1 cut(s)	766
PceI	AGGCCT	1 cut(s)	1768
PciSI	GCTCTTC	2 cut(s)	561, 2457
PctI	GAATGC	3 cut(s)	345, 1324, 2773
PdiI	GCCGGC	1 cut(s)	295
PdmI	GAANNNNTTC	1 cut(s)	1699
PfeI	GAWTC	8 cut(s)	132, 635, 1409, 1549, 1837, 2339, 2387, 2500
Pfl23II	CGTACG	1 cut(s)	1842
PflFI	GACNNNGTC	1 cut(s)	1954
PflMI	CCANNNNNTGG	1 cut(s)	653
PfoI	TCCNGGA	1 cut(s)	2334
PkrI	GCNGC	7 cut(s)	153, 198, 877, 1036, 1140, 1539, 1914
PleI	GAGTC	9 cut(s)	50, 109, 756, 979, 1709, 1724, 1948, 2357, 2851
PpsI	GAGTC	9 cut(s)	50, 109, 756, 979, 1709, 1724, 1948, 2357, 2851
PpuMI	RGGWCCY	5 cut(s)	163, 355, 2102, 2189, 2813
PshAI	GACNNNNGTC	1 cut(s)	1245
Psp124BI	GAGCTC	1 cut(s)	249
Psp5II	RGGWCCY	5 cut(s)	163, 355, 2102, 2189, 2813
Psp6I	CCWGG	6 cut(s)	765, 1511, 2185, 2230, 2334, 2602
PspFI	CCCAGC	3 cut(s)	105, 200, 1141
PspGI	CCWGG	6 cut(s)	765, 1511, 2185, 2230, 2334, 2602
PspLI	CGTACG	1 cut(s)	1842
PspOMI	GGGCCC	1 cut(s)	1679
PspPPI	RGGWCCY	5 cut(s)	163, 355, 2102, 2189, 2813
PstI	CTGCAG	1 cut(s)	2041
PstNI	CAGNNNCTG	1 cut(s)	2603
PsuI	RGATCY	2 cut(s)	812, 2837
PsyI	GACNNNGTC	1 cut(s)	1954
PvuII	CAGCTG	1 cut(s)	1141
RsaI	GTAC	9 cut(s)	483, 1278, 1351, 1723, 1844, 2070, 2109, 2590, 2762
RsaNI	GTAC	9 cut(s)	482, 1277, 1350, 1722, 1843, 2069, 2108, 2589, 2761
SacI	GAGCTC	1 cut(s)	249
SalI	GTCGAC	1 cut(s)	1948
SapI	GCTCTTC	2 cut(s)	561, 2457
SatI	GCNGC	7 cut(s)	152, 197, 876, 1035, 1139, 1538, 1913
ScaI	AGTACT	1 cut(s)	1351
SchI	GAGTC	9 cut(s)	51, 109, 757, 980, 1709, 1724, 1949, 2358, 2852
ScrFI	CCNGG	9 cut(s)	81, 359, 767, 1513, 2187, 2232, 2259, 2336, 2604
SduI	GDGCHC	6 cut(s)	142, 249, 1683, 1809, 2404, 2469
SexAI	ACCWGGT	1 cut(s)	2185
SfaNI	GCATC	5 cut(s)	330, 666, 1069, 1734, 2743
SfcI	CTRYAG	3 cut(s)	1294, 2037, 2343
Sfr274I	CTCGAG	1 cut(s)	350
SfuI	TTCGAA	1 cut(s)	260
SgrAI	CRCCGGYG	1 cut(s)	293
SinI	GGWCC	7 cut(s)	127, 163, 355, 771, 2102, 2189, 2813
SlaI	CTCGAG	1 cut(s)	350
SmlI	CTYRAG	5 cut(s)	146, 350, 1503, 2298, 2372
SmoI	CTYRAG	5 cut(s)	146, 350, 1503, 2298, 2372
SphI	GCATGC	1 cut(s)	2828
SseBI	AGGCCT	1 cut(s)	1768
SspI	AATATT	4 cut(s)	412, 677, 832, 2026
SspMI	CTAG	9 cut(s)	39, 801, 1157, 1287, 1359, 1655, 1707, 2247, 2421
SstI	GAGCTC	1 cut(s)	249
StuI	AGGCCT	1 cut(s)	1768
StyD4I	CCNGG	9 cut(s)	79, 357, 765, 1511, 2185, 2230, 2257, 2334, 2602
StyI	CCWWGG	3 cut(s)	1243, 1306, 1329
TaiI	ACGT	1 cut(s)	685
TaqI	TCGA	9 cut(s)	260, 351, 363, 643, 974, 1459, 1735, 1949, 2352
TaqII	GACCGA	2 cut(s)	144, 1421
TatI	WGTACW	3 cut(s)	481, 1349, 2760
TauI	GCSGC	1 cut(s)	199
TfiI	GAWTC	8 cut(s)	132, 635, 1409, 1549, 1837, 2339, 2387, 2500
TscAI	CASTG	5 cut(s)	387, 540, 1102, 1755, 2575
TseFI	GTSAC	3 cut(s)	1222, 1597, 2802
TseI	GCWGC	6 cut(s)	151, 875, 1034, 1138, 1537, 1912
Tsp45I	GTSAC	3 cut(s)	1222, 1597, 2802
TspDTI	ATGAA	8 cut(s)	882, 993, 1664, 1887, 1944, 2400, 2661, 2780
TspGWI	ACGGA	6 cut(s)	327, 675, 916, 1830, 2209, 2793
TspRI	CASTG	5 cut(s)	387, 540, 1102, 1755, 2575
Tth111I	GACNNNGTC	1 cut(s)	1954
Van91I	CCANNNNNTGG	1 cut(s)	653
VneI	GTGCAC	1 cut(s)	1805
VpaK11BI	GGWCC	7 cut(s)	127, 163, 355, 771, 2102, 2189, 2813
XagI	CCTNNNNNAGG	1 cut(s)	2372
XapI	RAATTY	3 cut(s)	1231, 1786, 2680
XbaI	TCTAGA	3 cut(s)	1156, 1286, 1654
XceI	RCATGY	4 cut(s)	1906, 2492, 2563, 2828
XhoI	CTCGAG	1 cut(s)	350
XmiI	GTMKAC	1 cut(s)	1949
XmnI	GAANNNNTTC	1 cut(s)	1699
XspI	CTAG	9 cut(s)	39, 801, 1157, 1287, 1359, 1655, 1707, 2247, 2421
ZrmI	AGTACT	1 cut(s)	1351
Zsp2I	ATGCAT	1 cut(s)	1084

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.