Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0009992

Physical Location & Seq

Genomic Coordinates Reverse (-)

93329133 .. 93334975

Gene Structure 5843 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00072170.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2928 bp

ATGGAGCTTTCCCGAGAAAACCCTAGACCCCTCGCTTTTCCGGCCACCAAGCCCATGCCCGACCTCGCTCCCAAGCCGCCGCCGCCGCCGCCGACCACGACTCCGATTATCGACCGCTACAAAGTCCTGCTCAAGCAGCGCGACGACGACCTCCGGGTCTCGCCCGAGGACGAAGTGTCGCCGCCGAGCACCGAGGAGATTGTGCAGCTCTACGAGATGCTCTTGTCCGAGCTCGTGTTCAATTCGAAGCCGATCATTACCGATCTCACTATCATCGCCGGCGAGCAGCGAGACCACGGCAAGGGCATCGCCGGCGCCATCTGCGCTCGGATTCTCGAGGTCCCAGTTGAGCACAAGCTGCCTTCGTTATATCTTTTAGACAGTATTGTAAAGAACATTGGCCGAGACTATGTCAGATACTTCTCTTCCCGTCTACCTGAGGTCTTCTGTGAGGCATACAGGCAAGTTCACCCCAACCAGCATTCTGCCATGCGCCACCTCTTTGGCACTTGGTCGGCAGTGTTTCCACCTTCAGTCCTTCGCAGGATTGAAGCGCAGCTGCAGTTTTCTCCACAGATAAACCAGCAATCGTCAGGTTTACCTCCTTTGAGAGCATCTGAATCTCCTCGGCCGGCTCATGGCATCCATGTCAATCCAAAATATTTACGTCAGTTGGAGACCTCAGATGTGGATAATGTTGGACCTCAAAGATTAAGCTCAACAGGAAATATGAGTCATTCAGATTTTCCTGTTGGGTCTAATCGTATACAGTCATCTTCAACTGTCAGACTTGCAAGATCTTCTTCACCTTCAAATATTGGCCTCGATGAATATGAAGTAGAAAATTCTCCTAAAAGGTTTGGTGACCGGGCTTCTCCATCTAATTCTGTGTATGATTATAGAGCAATCAGAGATGAGGAGCCAAATGAAAGGCGGAGAAAACATTATCTTGATGGTAGTCAGAACCGGTTAAATAATGGACTTGAACATCAAAGACCAAGAGCTTTGATTGATGCATATGGAAAAGACAGTGGGGATAGAAGTTTAAATGATAAGCCTTTGCATGTTGGGCGGCTTGGTGTAAATGGTCTAGATCACAAAGCAACTTCAATGTCATGGCAGAATACTGAGGAGGATGAGTTTGATTGGAAATCTGTCGGCCCACCTATAACCGAGCACACTAGGAGTAATGATTTCTTGCCTTCAAATATACCACCTTCTAGAAGCTACAGGGCCAGGCCTGGCCTCGGAACACTGAATGCAAGCTCCTTAGATTCTGATATTGGGAGTAGTTGGTCTACTCAGGCATACCTACCTTCCGAACAGTCCTCTGTAGTTGCTGAAGATCCAGTCCCCCCGCTTTCTTTTACTCGTGGATTCGCGGGTAGATTCCAGTCCGAGATAAATCATAATCAGGGTTCTCGCCACCCTCAAGAACCGTGGAATATGCCTTTCCATCCCTCTCAGCATTCACAGACTCTTCTTAATACCAAAGAAATAGGTAGAAATTTCCATATGCCTGGTATGTCTCTAGGTGGTGAGAAAGTGTCTACTGATGCCGATGTGCGACTTCACGGGCCAACATCAAGAATGGGTTCTGGTGCTGACTTTGTTAATGCTGACTCTCGGTTAGTTATACCAATGTCAGTGGGTTTAAGGCCTCCAGTAAACGTGCACAATTCTCACCCGCCGCCTGTGCACCCTATTTTTCCATTGCAAAACCAGAGGAGTCAGTATGGTTTCATAAATTCTGTCGATACTGCTAAGAATCAAGGTCCATATAAGTCTATGTATATGCCTGAGCAGCAGTTGGACGGTTATGAAAACAAGGATTTGGGGTTGGCAAAGCTAAGTCAGTTGACTAGTCAGAATGCCAGACTCATAGCTGTTAATCAGCGAAACCAGGCTCAGGTCAGTCCTTTCCAACCACAATTTCATCCACATCAGGAGCCACCTTATTCAGTGGTACCACGCGGATACAATTTGCAAGGGCAGGGTGGTACTGGTATCACAAATCCTGTACCTCGTCTACAGTTGGGCTTACCAACTCACTATACCCCAAACGCTAATTTGCGTGGGGACTCCTTGCCACCTCTACCACCTGGCCCCCTCCTCCCATGCAAGGTGTGTGATCTCATTGACAAATCCAAGTGGACTGCAGGAGTTGAGTTCAATGCAGACCTTCTTAAGGTACGTCATGAATCTGCAATAACTGCTCTGTATTATGATCTCCCAAGACAATGCACAACCTGTGGTCTCCGGTTCAAGTGCCAAGAGGAGCACAGTAGTCATATGGATTGGCATGTGACAAAGAACAGGATGTCTAAAAACCGTAAGCAGAAGCCCTCTCGTAAGTGGTTTGTAACTACAAGCATGTGGCTCAGTGGTGCAGAGGCTTTGGGAACTGATGCAGTTCCTGGCTTTTTACCTGCTGACACCATTGCGGAAAAGAAGAGTGATGAAGAATTGGCTGTTCCTGCTGATGAGGATCAGAATTCATGCGCGTTATGTGGAGAGCCTTTTGATGATTTTTATAGTGATGAGACAGAGGAGTGGATGTATAAAGGTGCTGTGTACTTGAATGCACCTCATGGGTCAACACCAGACATGGATAGGTCACAATTAGGTCCTATAGTGCATGCCAAATGCAGGCCGGAGTCCACTGATGTATCCCCTGGATGTTTTGGACAAGATGAAGGGGGGGATATGGAACTATTGAAGAGGGTAGTCAAAGAAAACGTTTGCGGAGTTGACATAGTTAGGAGGACTTCCATGTCCACTCATGTTCAAGGGAGGGAGAGAATTATTGAATCCATTCTATTGAATGGTTTTGTGCAAAACATATTTTGGCCGTCTTGTGCATTCTGCAGGGAATGCATTGTGGAACCACCTGTAAACAAAGACTTTGAAGGCTCAAGTGCACTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000993 GO:0001098 GO:0001099 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005849 GO:0006139 GO:0006351 GO:0006353 GO:0006366 GO:0006369 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009909 GO:0009911 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0018130 GO:0019438 GO:0019899 GO:0031123 GO:0031124 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0046483 GO:0048518 GO:0048580 GO:0048582 GO:0048831 GO:0050789 GO:0050793 GO:0051094 GO:0051239 GO:0051240 GO:0065007 GO:0070013 GO:0070063 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

CTD_bind

Protein Analysis

975

Amino Acids

108.69

Weight (kDa)

6.26

Isoelectric Point (pI)

58.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CID	PF04818	74 - 180	1.6e-11	CID domain
zf_PCFS4	PF23228	820 - 886	2.4e-29	PCFS4 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009992)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04885
fragaria_vesca	FvH4_3g02510 FvH4_3g02510 FvH4_3g02510 FvH4_3g02510
malus_domestica	MD05G1342300.v1.1 MD10G1315900.v1.1
prunus_persica	Prupe.4G024700_v2.0.a1
pyrus_communis	pycom05g31160 pycom10g26810
rosa_chinensis	RchiOBHm_Chr5g0003821
rosa_laevigata	RLG00000031178
rosa_multiflora	Rmu_sc0004212.1_g000001
rosa_roxburghii	Rroxscaffold_1G00072170
rosa_rugosa	Rorug04G0406900 Rorug04G0407000
rosa_samantha	Rh5AG034400 Rh5BG032700 Rh5CG036800 Rh5DG033100
rosa_wichuraiana	Rw5G003150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	155, 2773
Acc36I	ACCTGC	1 cut(s)	2437
Acc65I	GGTACC	1 cut(s)	1966
AccB1I	GGYRCC	2 cut(s)	314, 1966
AccI	GTMKAC	5 cut(s)	433, 766, 1298, 1550, 2029
AccII	CGCG	4 cut(s)	141, 1382, 1974, 2504
AclI	AACGTT	1 cut(s)	2739
AclWI	GGATC	2 cut(s)	1340, 2496
AcoI	YGGCCR	4 cut(s)	42, 400, 629, 2849
AcsI	RAATTY	4 cut(s)	844, 1507, 1747, 2494
AcuI	CTGAAG	2 cut(s)	516, 1362
AcyI	GRCGYC	1 cut(s)	315
AfaI	GTAC	5 cut(s)	1968, 2002, 2022, 2193, 2576
AfiI	CCNNNNNNNGG	7 cut(s)	301, 638, 1247, 1909, 2122, 2188, 2649
AflII	CTTAAG	1 cut(s)	2186
AgeI	ACCGGT	1 cut(s)	966
AhdI	GACNNNNNGTC	1 cut(s)	175
AhlI	ACTAGT	1 cut(s)	1862
AjnI	CCWGG	7 cut(s)	1235, 1240, 1519, 1902, 2101, 2416, 2674
AjuI	GAANNNNNNNTTGG	2 cut(s)	1917, 1949
Alw21I	GWGCWC	8 cut(s)	191, 234, 354, 1179, 1677, 1701, 2283, 2923
Alw26I	GTCTC	7 cut(s)	163, 285, 399, 671, 1533, 2261, 2537
Alw44I	GTGCAC	3 cut(s)	1673, 1697, 2919
AlwI	GGATC	2 cut(s)	1340, 2496
AlwNI	CAGNNNCTG	1 cut(s)	2417
Ama87I	CYCGRG	3 cut(s)	12, 164, 335
ApaLI	GTGCAC	3 cut(s)	1673, 1697, 2919
ApeKI	GCWGC	7 cut(s)	136, 205, 286, 358, 556, 559, 1804
ApoI	RAATTY	4 cut(s)	844, 1507, 1747, 2494
AsiGI	ACCGGT	1 cut(s)	966
Asp700I	GAANNNNTTC	2 cut(s)	1594, 2814
Asp718I	GGTACC	1 cut(s)	1966
AspLEI	GCGC	6 cut(s)	141, 317, 326, 495, 556, 2504
AspS9I	GGNCC	8 cut(s)	340, 701, 1160, 1233, 1577, 1775, 2105, 2627
AsuC2I	CCSGG	2 cut(s)	155, 869
AsuHPI	GGTGA	5 cut(s)	461, 798, 875, 1550, 1676
AsuII	TTCGAA	1 cut(s)	245
AvaI	CYCGRG	3 cut(s)	12, 164, 335
AvaII	GGWCC	4 cut(s)	340, 701, 1775, 2627
AxyI	CCTNAGG	1 cut(s)	438
BaeGI	GKGCMC	3 cut(s)	1677, 1701, 2923
BaeI	ACNNNNGTAYC	2 cut(s)	2652, 2685
BanI	GGYRCC	2 cut(s)	314, 1966
BanII	GRGCYC	1 cut(s)	234
BauI	CACGAG	2 cut(s)	233, 1371
BbsI	GAAGAC	1 cut(s)	436
Bbv12I	GWGCWC	8 cut(s)	191, 234, 354, 1179, 1677, 1701, 2283, 2923
BbvI	GCAGC	7 cut(s)	148, 217, 298, 345, 546, 568, 1816
BccI	CCATC	4 cut(s)	326, 886, 947, 1464
BceAI	ACGGC	2 cut(s)	313, 2836
BcgI	CGANNNNNNTGC	2 cut(s)	289, 323
BciT130I	CCWGG	7 cut(s)	1237, 1242, 1521, 1904, 2103, 2418, 2676
BciVI	GTATCC	2 cut(s)	1970, 2680
BcnI	CCSGG	2 cut(s)	155, 869
BcoDI	GTCTC	7 cut(s)	163, 285, 399, 671, 1533, 2261, 2537
BcuI	ACTAGT	1 cut(s)	1862
BfaI	CTAG	6 cut(s)	24, 1091, 1182, 1221, 1532, 1863
BfmI	CTRYAG	7 cut(s)	560, 1228, 1332, 2030, 2157, 2631, 2866
BfoI	RGCGCY	1 cut(s)	318
BfrI	CTTAAG	1 cut(s)	2186
BfuAI	ACCTGC	1 cut(s)	2437
BfuI	GTATCC	2 cut(s)	1970, 2680
BglII	AGATCT	1 cut(s)	797
Bme1390I	CCNGG	9 cut(s)	155, 869, 1237, 1242, 1521, 1904, 2103, 2418, 2676
Bme18I	GGWCC	4 cut(s)	340, 701, 1775, 2627
BmeRI	GACNNNNNGTC	1 cut(s)	175
BmeT110I	CYCGRG	3 cut(s)	12, 164, 335
BmgT120I	GGNCC	8 cut(s)	340, 701, 1160, 1233, 1577, 1775, 2105, 2627
BmiI	GGNNCC	7 cut(s)	316, 342, 921, 1950, 1968, 2107, 2886
BmrFI	CCNGG	9 cut(s)	155, 869, 1237, 1242, 1521, 1904, 2103, 2418, 2676
BmrI	ACTGGG	1 cut(s)	338
BmsI	GCATC	7 cut(s)	207, 315, 623, 651, 1003, 1546, 2398
BmuI	ACTGGG	1 cut(s)	338
BpiI	GAAGAC	1 cut(s)	436
BpmI	CTGGAG	1 cut(s)	1647
Bpu10I	CCTNAGC	2 cut(s)	1800, 1908
Bpu14I	TTCGAA	1 cut(s)	245
BpuEI	CTTGAG	3 cut(s)	116, 1416, 2899
BpuMI	CCSGG	2 cut(s)	155, 869
BsaBI	GATNNNNATC	1 cut(s)	2487
BsaHI	GRCGYC	1 cut(s)	315
BsaI	GGTCTC	4 cut(s)	163, 285, 671, 2261
BsaJI	CCNNGG	7 cut(s)	165, 192, 295, 626, 1246, 1439, 2674
BsaWI	WCCGGW	2 cut(s)	966, 2259
BsaXI	ACNNNNNCTCC	6 cut(s)	85, 115, 1124, 1154, 2543, 2573
Bsc4I	CCNNNNNNNGG	7 cut(s)	301, 638, 1247, 1909, 2122, 2188, 2649
Bse118I	RCCGGY	4 cut(s)	278, 311, 631, 966
Bse1I	ACTGG	5 cut(s)	344, 1349, 1393, 1664, 2008
Bse21I	CCTNAGG	1 cut(s)	438
Bse3DI	GCAATG	2 cut(s)	1712, 2439
Bse8I	GATNNNNATC	1 cut(s)	2487
BseBI	CCWGG	7 cut(s)	1237, 1242, 1521, 1904, 2103, 2418, 2676
BseDI	CCNNGG	7 cut(s)	165, 192, 295, 626, 1246, 1439, 2674
BseGI	GGATG	7 cut(s)	642, 1141, 1456, 1936, 2325, 2562, 2684
BseJI	GATNNNNATC	1 cut(s)	2487
BseLI	CCNNNNNNNGG	7 cut(s)	301, 638, 1247, 1909, 2122, 2188, 2649
BseMI	GCAATG	2 cut(s)	1712, 2439
BseMII	CTCAG	8 cut(s)	429, 696, 1119, 1316, 1478, 1791, 1922, 2395
BseNI	ACTGG	5 cut(s)	344, 1349, 1393, 1664, 2008
BseRI	GAGGAG	8 cut(s)	209, 615, 932, 1145, 1741, 2102, 2291, 2564
BseSI	GKGCMC	3 cut(s)	1677, 1701, 2923
BseX3I	CGGCCG	1 cut(s)	629
BseXI	GCAGC	7 cut(s)	148, 217, 298, 345, 546, 568, 1816
BsgI	GTGCAG	2 cut(s)	224, 2409
Bsh1236I	CGCG	4 cut(s)	141, 1382, 1974, 2504
Bsh1285I	CGRYCG	2 cut(s)	115, 632
BshNI	GGYRCC	2 cut(s)	314, 1966
BshTI	ACCGGT	1 cut(s)	966
BsiEI	CGRYCG	2 cut(s)	115, 632
BsiHKAI	GWGCWC	8 cut(s)	191, 234, 354, 1179, 1677, 1701, 2283, 2923
BsiHKCI	CYCGRG	3 cut(s)	12, 164, 335
BsiSI	CCGG	9 cut(s)	41, 154, 279, 312, 632, 868, 967, 2260, 2654
BslFI	GGGAC	3 cut(s)	326, 1337, 2093
BslI	CCNNNNNNNGG	7 cut(s)	301, 638, 1247, 1909, 2122, 2188, 2649
BsmAI	GTCTC	7 cut(s)	163, 285, 399, 671, 1533, 2261, 2537
BsmFI	GGGAC	3 cut(s)	326, 1337, 2093
BsmI	GAATGC	7 cut(s)	481, 1264, 1468, 1876, 2587, 2861, 2879
Bso31I	GGTCTC	4 cut(s)	163, 285, 671, 2261
BsoBI	CYCGRG	3 cut(s)	12, 164, 335
Bsp119I	TTCGAA	1 cut(s)	245
Bsp1286I	GDGCHC	8 cut(s)	191, 234, 354, 1179, 1677, 1701, 2283, 2923
Bsp143I	GATC	8 cut(s)	252, 262, 797, 1093, 1345, 2131, 2227, 2488
BspCNI	CTCAG	8 cut(s)	430, 695, 1120, 1315, 1477, 1792, 1921, 2394
BspFNI	CGCG	4 cut(s)	141, 1382, 1974, 2504
BspHI	TCATGA	1 cut(s)	2197
BspLI	GGNNCC	7 cut(s)	316, 342, 921, 1950, 1968, 2107, 2886
BspMAI	CTGCAG	3 cut(s)	564, 2161, 2870
BspMI	ACCTGC	1 cut(s)	2437
BspPI	GGATC	2 cut(s)	1340, 2496
BspT104I	TTCGAA	1 cut(s)	245
BspT107I	GGYRCC	2 cut(s)	314, 1966
BspTI	CTTAAG	1 cut(s)	2186
BspTNI	GGTCTC	4 cut(s)	163, 285, 671, 2261
BsrDI	GCAATG	2 cut(s)	1712, 2439
BsrFI	RCCGGY	4 cut(s)	278, 311, 631, 966
BsrI	ACTGG	5 cut(s)	344, 1349, 1393, 1664, 2008
BssAI	RCCGGY	4 cut(s)	278, 311, 631, 966
BssECI	CCNNGG	7 cut(s)	165, 192, 295, 626, 1246, 1439, 2674
BssMI	GATC	8 cut(s)	252, 262, 797, 1093, 1345, 2131, 2227, 2488
BssNAI	GTATAC	1 cut(s)	767
BssNI	GRCGYC	1 cut(s)	315
BssSI	CACGAG	2 cut(s)	233, 1371
Bst1107I	GTATAC	1 cut(s)	767
Bst2BI	CACGAG	2 cut(s)	233, 1371
Bst2UI	CCWGG	7 cut(s)	1237, 1242, 1521, 1904, 2103, 2418, 2676
Bst6I	CTCTTC	4 cut(s)	430, 1485, 2447, 2714
BstACI	GRCGYC	1 cut(s)	315
BstAFI	CTTAAG	1 cut(s)	2186
BstAPI	GCANNNNNTGC	3 cut(s)	358, 2213, 2874
BstBI	TTCGAA	1 cut(s)	245
BstC8I	GCNNGC	7 cut(s)	280, 284, 313, 633, 1264, 2640, 2651
BstDSI	CCRYGG	2 cut(s)	295, 1439
BstEII	GGTNACC	1 cut(s)	863
BstENI	CCTNNNNNAGG	1 cut(s)	2186
BstF5I	GGATG	7 cut(s)	642, 1141, 1456, 1936, 2325, 2562, 2684
BstFNI	CGCG	4 cut(s)	141, 1382, 1974, 2504
BstH2I	RGCGCY	1 cut(s)	318
BstHHI	GCGC	6 cut(s)	141, 317, 326, 495, 556, 2504
BstKTI	GATC	8 cut(s)	255, 265, 800, 1096, 1348, 2134, 2230, 2491
BstMAI	GTCTC	7 cut(s)	163, 285, 399, 671, 1533, 2261, 2537
BstMBI	GATC	8 cut(s)	252, 262, 797, 1093, 1345, 2131, 2227, 2488
BstMCI	CGRYCG	2 cut(s)	115, 632
BstNI	CCWGG	7 cut(s)	1237, 1242, 1521, 1904, 2103, 2418, 2676
BstNSI	RCATGY	4 cut(s)	1067, 2306, 2377, 2642
BstPI	GGTNACC	1 cut(s)	863
BstSCI	CCNGG	9 cut(s)	153, 867, 1235, 1240, 1519, 1902, 2101, 2416, 2674
BstSFI	CTRYAG	7 cut(s)	560, 1228, 1332, 2030, 2157, 2631, 2866
BstSLI	GKGCMC	3 cut(s)	1677, 1701, 2923
BstUI	CGCG	4 cut(s)	141, 1382, 1974, 2504
BstV1I	GCAGC	7 cut(s)	148, 217, 298, 345, 546, 568, 1816
BstV2I	GAAGAC	1 cut(s)	436
BstX2I	RGATCY	2 cut(s)	797, 1345
BstXI	CCANNNNNNTGG	2 cut(s)	503, 1520
BstYI	RGATCY	2 cut(s)	797, 1345
BstZ17I	GTATAC	1 cut(s)	767
BstZI	CGGCCG	1 cut(s)	629
Bsu36I	CCTNAGG	1 cut(s)	438
BsuI	GTATCC	2 cut(s)	1970, 2680
BtgI	CCRYGG	2 cut(s)	295, 1439
BtgZI	GCGATG	2 cut(s)	259, 292
BtsCI	GGATG	7 cut(s)	642, 1141, 1456, 1936, 2325, 2562, 2684
BtsI	GCAGTG	1 cut(s)	525
BtsIMutI	CAGTG	8 cut(s)	525, 1036, 1253, 1653, 1968, 2389, 2661, 2921
BveI	ACCTGC	1 cut(s)	2437
Cac8I	GCNNGC	7 cut(s)	280, 284, 313, 633, 1264, 2640, 2651
CaiI	CAGNNNCTG	1 cut(s)	2417
CciI	TCATGA	1 cut(s)	2197
CfoI	GCGC	6 cut(s)	141, 317, 326, 495, 556, 2504
Cfr10I	RCCGGY	4 cut(s)	278, 311, 631, 966
Cfr13I	GGNCC	8 cut(s)	340, 701, 1160, 1233, 1577, 1775, 2105, 2627
Csp6I	GTAC	5 cut(s)	1967, 2001, 2021, 2192, 2575
CspAI	ACCGGT	1 cut(s)	966
CspCI	CAANNNNNGTGG	4 cut(s)	1421, 1456, 1629, 1664
CviQI	GTAC	5 cut(s)	1967, 2001, 2021, 2192, 2575
DinI	GGCGCC	1 cut(s)	316
DpnI	GATC	8 cut(s)	254, 264, 799, 1095, 1347, 2133, 2229, 2490
DpnII	GATC	8 cut(s)	252, 262, 797, 1093, 1345, 2131, 2227, 2488
DraI	TTTAAA	1 cut(s)	1047
DrdI	GACNNNNNNGTC	2 cut(s)	155, 2773
DriI	GACNNNNNGTC	1 cut(s)	175
DseDI	GACNNNNNNGTC	2 cut(s)	155, 2773
EaeI	YGGCCR	4 cut(s)	42, 400, 629, 2849
EagI	CGGCCG	1 cut(s)	629
Eam1104I	CTCTTC	4 cut(s)	430, 1485, 2447, 2714
Eam1105I	GACNNNNNGTC	1 cut(s)	175
EarI	CTCTTC	4 cut(s)	430, 1485, 2447, 2714
EciI	GGCGGA	1 cut(s)	949
Ecl136II	GAGCTC	1 cut(s)	232
EclXI	CGGCCG	1 cut(s)	629
Eco147I	AGGCCT	2 cut(s)	1240, 1660
Eco24I	GRGCYC	1 cut(s)	234
Eco31I	GGTCTC	4 cut(s)	163, 285, 671, 2261
Eco47I	GGWCC	4 cut(s)	340, 701, 1775, 2627
Eco52I	CGGCCG	1 cut(s)	629
Eco53kI	GAGCTC	1 cut(s)	232
Eco57I	CTGAAG	2 cut(s)	516, 1362
Eco81I	CCTNAGG	1 cut(s)	438
Eco88I	CYCGRG	3 cut(s)	12, 164, 335
Eco91I	GGTNACC	1 cut(s)	863
EcoICRI	GAGCTC	1 cut(s)	232
EcoNI	CCTNNNNNAGG	1 cut(s)	2186
EcoO109I	RGGNCCY	2 cut(s)	340, 2627
EcoO65I	GGTNACC	1 cut(s)	863
EcoRI	GAATTC	1 cut(s)	2494
EcoRII	CCWGG	7 cut(s)	1235, 1240, 1519, 1902, 2101, 2416, 2674
EcoT22I	ATGCAT	2 cut(s)	1018, 2879
EcoT38I	GRGCYC	1 cut(s)	234
EgeI	GGCGCC	1 cut(s)	316
EheI	GGCGCC	1 cut(s)	316
FalI	AAGNNNNNCTT	2 cut(s)	787, 819
FaqI	GGGAC	3 cut(s)	326, 1337, 2093
FauI	CCCGC	3 cut(s)	1365, 1375, 1695
FauNDI	CATATG	3 cut(s)	1018, 1515, 2292
FblI	GTMKAC	5 cut(s)	433, 766, 1298, 1550, 2029
FokI	GGATG	7 cut(s)	629, 1148, 1443, 1923, 2332, 2569, 2691
FriOI	GRGCYC	1 cut(s)	234
FspBI	CTAG	6 cut(s)	24, 1091, 1182, 1221, 1532, 1863
GlaI	GCGC	6 cut(s)	140, 316, 325, 494, 555, 2503
GsuI	CTGGAG	1 cut(s)	1647
HaeII	RGCGCY	1 cut(s)	318
HapII	CCGG	9 cut(s)	41, 154, 279, 312, 632, 868, 967, 2260, 2654
HhaI	GCGC	6 cut(s)	141, 317, 326, 495, 556, 2504
Hin1I	GRCGYC	1 cut(s)	315
Hin6I	GCGC	6 cut(s)	139, 315, 324, 493, 554, 2502
HinP1I	GCGC	6 cut(s)	139, 315, 324, 493, 554, 2502
HincII	GTYRAC	3 cut(s)	1860, 2598, 2752
HindII	GTYRAC	3 cut(s)	1860, 2598, 2752
HpaII	CCGG	9 cut(s)	41, 154, 279, 312, 632, 868, 967, 2260, 2654
HphI	GGTGA	5 cut(s)	461, 798, 875, 1550, 1676
Hpy188III	TCNNGA	9 cut(s)	12, 335, 950, 1091, 1221, 1433, 1587, 1946, 2198
Hpy99I	CGWCG	2 cut(s)	146, 149
HpyCH4IV	ACGT	4 cut(s)	667, 1671, 2194, 2739
HpySE526I	ACGT	4 cut(s)	667, 1671, 2194, 2739
Hsp92I	GRCGYC	1 cut(s)	315
HspAI	GCGC	6 cut(s)	139, 315, 324, 493, 554, 2502
KasI	GGCGCC	1 cut(s)	314
KpnI	GGTACC	1 cut(s)	1970
KroI	GCCGGC	3 cut(s)	278, 311, 631
KroNI	GCCGGC	3 cut(s)	280, 313, 633
Kzo9I	GATC	8 cut(s)	252, 262, 797, 1093, 1345, 2131, 2227, 2488
LmnI	GCTCC	6 cut(s)	4, 73, 919, 1271, 1948, 2278
Lsp1109I	GCAGC	7 cut(s)	148, 217, 298, 345, 546, 568, 1816
LweI	GCATC	7 cut(s)	207, 315, 623, 651, 1003, 1546, 2398
MaeI	CTAG	6 cut(s)	24, 1091, 1182, 1221, 1532, 1863
MaeII	ACGT	4 cut(s)	667, 1671, 2194, 2739
MaeIII	GTNAC	4 cut(s)	863, 2305, 2362, 2616
MalI	GATC	8 cut(s)	254, 264, 799, 1095, 1347, 2133, 2229, 2490
MboI	GATC	8 cut(s)	252, 262, 797, 1093, 1345, 2131, 2227, 2488
MflI	RGATCY	2 cut(s)	797, 1345
MhlI	GDGCHC	8 cut(s)	191, 234, 354, 1179, 1677, 1701, 2283, 2923
Mly113I	GGCGCC	1 cut(s)	315
MlyI	GAGTC	8 cut(s)	94, 742, 1471, 1616, 1738, 1872, 2075, 2666
MmeI	TCCRAC	4 cut(s)	654, 679, 1791, 1948
Mph1103I	ATGCAT	2 cut(s)	1018, 2879
MreI	CGCCGGCG	2 cut(s)	278, 311
MroNI	GCCGGC	3 cut(s)	278, 311, 631
MroXI	GAANNNNTTC	2 cut(s)	1594, 2814
MseI	TTAA	8 cut(s)	713, 971, 1046, 1485, 1614, 1655, 1890, 2187
MspA1I	CMGCKG	1 cut(s)	559
MspCI	CTTAAG	1 cut(s)	2186
MspI	CCGG	9 cut(s)	41, 154, 279, 312, 632, 868, 967, 2260, 2654
MspR9I	CCNGG	9 cut(s)	155, 869, 1237, 1242, 1521, 1904, 2103, 2418, 2676
Mva1269I	GAATGC	7 cut(s)	481, 1264, 1468, 1876, 2587, 2861, 2879
MvaI	CCWGG	7 cut(s)	1237, 1242, 1521, 1904, 2103, 2418, 2676
MvnI	CGCG	4 cut(s)	141, 1382, 1974, 2504
NaeI	GCCGGC	3 cut(s)	280, 313, 633
NarI	GGCGCC	1 cut(s)	315
NciI	CCSGG	2 cut(s)	155, 869
NdeI	CATATG	3 cut(s)	1018, 1515, 2292
NdeII	GATC	8 cut(s)	252, 262, 797, 1093, 1345, 2131, 2227, 2488
NgoMIV	GCCGGC	3 cut(s)	278, 311, 631
NlaIV	GGNNCC	7 cut(s)	316, 342, 921, 1950, 1968, 2107, 2886
NmeAIII	GCCGAG	3 cut(s)	210, 428, 607
NmuCI	GTSAC	3 cut(s)	863, 2305, 2616
NsiI	ATGCAT	2 cut(s)	1018, 2879
NspI	RCATGY	4 cut(s)	1067, 2306, 2377, 2642
NspV	TTCGAA	1 cut(s)	245
PaeI	GCATGC	1 cut(s)	2642
PaeR7I	CTCGAG	1 cut(s)	335
PagI	TCATGA	1 cut(s)	2197
PceI	AGGCCT	2 cut(s)	1240, 1660
PctI	GAATGC	7 cut(s)	481, 1264, 1468, 1876, 2587, 2861, 2879
PdiI	GCCGGC	3 cut(s)	280, 313, 633
PdmI	GAANNNNTTC	2 cut(s)	1594, 2814
PfeI	GAWTC	8 cut(s)	331, 620, 1274, 1377, 1389, 1768, 2201, 2810
PflFI	GACNNNGTC	1 cut(s)	410
PinAI	ACCGGT	1 cut(s)	966
PleI	GAGTC	8 cut(s)	94, 741, 1471, 1616, 1737, 1872, 2075, 2665
PluTI	GGCGCC	1 cut(s)	318
PpsI	GAGTC	8 cut(s)	94, 741, 1471, 1616, 1737, 1872, 2075, 2665
PpuMI	RGGWCCY	2 cut(s)	340, 2627
Psp124BI	GAGCTC	1 cut(s)	234
Psp1406I	AACGTT	1 cut(s)	2739
Psp5II	RGGWCCY	2 cut(s)	340, 2627
Psp6I	CCWGG	7 cut(s)	1235, 1240, 1519, 1902, 2101, 2416, 2674
PspEI	GGTNACC	1 cut(s)	863
PspGI	CCWGG	7 cut(s)	1235, 1240, 1519, 1902, 2101, 2416, 2674
PspN4I	GGNNCC	7 cut(s)	316, 342, 921, 1950, 1968, 2107, 2886
PspPI	GGNCC	8 cut(s)	340, 701, 1160, 1233, 1577, 1775, 2105, 2627
PspPPI	RGGWCCY	2 cut(s)	340, 2627
PstI	CTGCAG	3 cut(s)	564, 2161, 2870
PstNI	CAGNNNCTG	1 cut(s)	2417
PsuI	RGATCY	2 cut(s)	797, 1345
PsyI	GACNNNGTC	1 cut(s)	410
PvuII	CAGCTG	1 cut(s)	559
RsaI	GTAC	5 cut(s)	1968, 2002, 2022, 2193, 2576
RsaNI	GTAC	5 cut(s)	1967, 2001, 2021, 2192, 2575
SacI	GAGCTC	1 cut(s)	234
SaqAI	TTAA	8 cut(s)	713, 971, 1046, 1485, 1614, 1655, 1890, 2187
Sau3AI	GATC	8 cut(s)	252, 262, 797, 1093, 1345, 2131, 2227, 2488
Sau96I	GGNCC	8 cut(s)	340, 701, 1160, 1233, 1577, 1775, 2105, 2627
SchI	GAGTC	8 cut(s)	94, 742, 1471, 1616, 1738, 1872, 2075, 2666
ScrFI	CCNGG	9 cut(s)	155, 869, 1237, 1242, 1521, 1904, 2103, 2418, 2676
SduI	GDGCHC	8 cut(s)	191, 234, 354, 1179, 1677, 1701, 2283, 2923
SfaNI	GCATC	7 cut(s)	207, 315, 623, 651, 1003, 1546, 2398
SfcI	CTRYAG	7 cut(s)	560, 1228, 1332, 2030, 2157, 2631, 2866
SfoI	GGCGCC	1 cut(s)	316
Sfr274I	CTCGAG	1 cut(s)	335
SfuI	TTCGAA	1 cut(s)	245
SgrAI	CRCCGGYG	2 cut(s)	278, 311
SinI	GGWCC	4 cut(s)	340, 701, 1775, 2627
SlaI	CTCGAG	1 cut(s)	335
SmlI	CTYRAG	5 cut(s)	131, 335, 1431, 2186, 2914
SmoI	CTYRAG	5 cut(s)	131, 335, 1431, 2186, 2914
SpeI	ACTAGT	1 cut(s)	1862
SphI	GCATGC	1 cut(s)	2642
SseBI	AGGCCT	2 cut(s)	1240, 1660
SspDI	GGCGCC	1 cut(s)	314
SspI	AATATT	2 cut(s)	662, 817
SspMI	CTAG	6 cut(s)	24, 1091, 1182, 1221, 1532, 1863
SstI	GAGCTC	1 cut(s)	234
StuI	AGGCCT	2 cut(s)	1240, 1660
StyD4I	CCNGG	9 cut(s)	153, 867, 1235, 1240, 1519, 1902, 2101, 2416, 2674
TaiI	ACGT	4 cut(s)	670, 1674, 2197, 2742
TaqI	TCGA	5 cut(s)	111, 245, 336, 825, 1755
TatI	WGTACW	1 cut(s)	2574
TauI	GCSGC	8 cut(s)	79, 82, 85, 88, 91, 184, 1075, 1693
TfiI	GAWTC	8 cut(s)	331, 620, 1274, 1377, 1389, 1768, 2201, 2810
Tru1I	TTAA	8 cut(s)	713, 971, 1046, 1485, 1614, 1655, 1890, 2187
Tru9I	TTAA	8 cut(s)	713, 971, 1046, 1485, 1614, 1655, 1890, 2187
TscAI	CASTG	8 cut(s)	525, 1036, 1260, 1653, 1968, 2389, 2668, 2928
TseFI	GTSAC	3 cut(s)	863, 2305, 2616
TseI	GCWGC	7 cut(s)	136, 205, 286, 358, 556, 559, 1804
Tsp45I	GTSAC	3 cut(s)	863, 2305, 2616
TspRI	CASTG	8 cut(s)	525, 1036, 1260, 1653, 1968, 2389, 2668, 2928
Tth111I	GACNNNGTC	1 cut(s)	410
Vha464I	CTTAAG	1 cut(s)	2186
VneI	GTGCAC	3 cut(s)	1673, 1697, 2919
VpaK11BI	GGWCC	4 cut(s)	340, 701, 1775, 2627
XagI	CCTNNNNNAGG	1 cut(s)	2186
XapI	RAATTY	4 cut(s)	844, 1507, 1747, 2494
XbaI	TCTAGA	2 cut(s)	1090, 1220
XceI	RCATGY	4 cut(s)	1067, 2306, 2377, 2642
XhoI	CTCGAG	1 cut(s)	335
XmiI	GTMKAC	5 cut(s)	433, 766, 1298, 1550, 2029
XmnI	GAANNNNTTC	2 cut(s)	1594, 2814
XspI	CTAG	6 cut(s)	24, 1091, 1182, 1221, 1532, 1863
Zsp2I	ATGCAT	2 cut(s)	1018, 2879

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.