MD11G1036300.v1.1

AI-2E family transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0004461
Physical Location & Seq
Forward (+)
3149034 .. 3151665
2632 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1036300.v1.1.491

Sequence Viewer

Length: 1956 bp
ATGGCTTTAGTTGCCTACTCAGACCCAAGCTCCGAACCCCCCTCAGCAAGCCCTGCTTGGCAAGACATGTTCCGATCAGCCTCAATCCGAAAATCCTCAGCCCCAGAGCCCCAGGCGCCCGAACCCCAGGCGCCGCCGCCCAAGGAACCGCCAAAATCCGCCGACCCAGATCAGAAAACCCCCATCTCCGGCGATCCCCAGGTACGATTAGCCCTCTACATAACTATGGCCCACGCCGGCCTCGCCTTTACTATTTTCATATTTTACGCCGTCGGCAAGCTGTTAGAGGAATACTTGCGGCCAATTCAATGGGCGGTGCTCTGCTCGATTCCTCTGAGAGGAATACAGCAAACCCTTGTGGGGTTTTGGTCGGAGCCCTTGAGGCTTGGCCTCACTGAAACTGTTCTGGCAGTTCCGGTGGCCATTTTTCGGGTTTTCGTAGGTACCCTTATCGAAATTAGGGAGGTTTGCTTTCGGGTTTTTCTCCGAAAGCCGAAATCTGACTACAGGAGGAGGCACCGGAGTGAGTTCTCTAAGTTGTTAAGGTGGTTGGTGTCGTTTTGGATACTTATACTTGCTTATGAGAAAATTGGTGGTGTTGCTTCTCTGGCAATTCTTGGACTAGGCTTTATGTTTAGTGCCAAAGGCGTGGAGTCGACAATGTCAACTGTTTCCTCTTTGAGATGCAGTAGCTTTCGCCGTAGCCCGATTAGTGCGTTTTTCACCAGAAGGATATTGACAAGGTTGGAGACCATCGTTGCAATTGGGTTGATGTTTTCTATGATTTTAGGATTTTTGGTTGGTGTCACCTTTTTTTCATATAAGATTGGGATCGAGAGCAAAGATGCAGTGATTTCGTTGAAGTTACATGTGGAAGAGAGCAATTACACGGAGAAGATTGGCGTTAAGCAGTGGATGGAGGAGAATGATGTGCCTGGAATGGTGGATAGGTACAGTTCAAAGTTTTATGAAACTGTGTCGGACCAGCTAGATAGTTTGGCGTTGCAGTATAATATGACAGAATTGGCAACTGGAATTAAGCATTTTGTAGCAAGACAGTCTGCTAATTCATCCGGGCCTTCAACAGCTTTAGCTAGCCCGTCTCAGTACACAGAGAAACTTGTGAGTTTGAGGGATCGGATTAGTAAGAGGGAATGGGGACTTATGTACACAGAGGTTGATGCAATTGTTAGGGAATTGGTAATCACCAGAGAGGATTTAGTCGAGAAGGCAAAAGGGTTTGCCATGCGAGGTATGGATGTATCACAGCGCATACTTGCTAGCGGTACATCAGTCCTTGGAGGCAGCGCAAAACTCATGTTCTCTATCGGTAGTTCCATTGTTTCTGGAGCTGCTGAGGTTTTCAATTTTGTGTCTCAGTTAATGGTGTTCTTCTGGGTATTGTATTATCTGATCACATCCAAATCTGGCGGAGTGACTGAACAAGCAATGAGTATGCTTCCAATTTCAAAGTCCGCAAGGGTTAGATGCGTTGAAGTTCTTGATAATGCTATATCTGGTGTTCTTTTGGCTACAGCCGAGATTGCAATTTTCCAGGGATGTCTCACATGGCTTTTATTGACGCTATTCAAAATACATTTCTTGTACACGTCCACCATTCTTGCAATCCTCAGTTCTGTGATACCAATCTTTCCATCGTGGTTTGCAACTATCCCTGCAGCTCTGCAACTGGTGCTGGAAGGTAGATATATAATAGCCGTCACCTTGTCCGTTATTCATCTCGTGCTTATGGATTACGGAGCGTCTGAAATTCAGGAGGACATACCTGGCCATAGTCAATATCTTACCGGACTCAGCATCATCGGTGGAATGACTTTGTTCCCATCCGCATTCGAGGGTGCAATCATGGGTCCGCTCATAACAACGGTTGTGATTGCTATTAAGGATTTGTACGTGGAATTTGTTCTGGACGAAACCAAGGATAAAGCCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AI-2E_transport

Protein Analysis

652

Amino Acids

72.0

Weight (kDa)

7.61

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AI-2E_transport PF01594 442 - 634 2.2e-09 AI-2E family transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 443
AccB1I GGYRCC 4 cut(s) 115, 130, 443, 516
AccBSI CCGCTC 1 cut(s) 1876
AccI GTMKAC 1 cut(s) 656
AclWI GGATC 3 cut(s) 188, 839, 1143
AcoI YGGCCR 3 cut(s) 299, 420, 1789
AcsI RAATTY 2 cut(s) 1770, 1919
AcyI GRCGYC 2 cut(s) 116, 131
AfaI GTAC 8 cut(s) 204, 445, 953, 1109, 1169, 1288, 1607, 1913
AfiI CCNNNNNNNGG 4 cut(s) 188, 338, 360, 429
AflIII ACRYGT 3 cut(s) 66, 868, 1608
AgsI TTSAA 8 cut(s) 308, 862, 960, 1083, 1366, 1470, 1496, 1591
AjiI CACGTC 1 cut(s) 1611
AjnI CCWGG 6 cut(s) 111, 126, 198, 934, 1554, 1786
AleI CACNNNNGTG 1 cut(s) 522
AluBI AGCT 8 cut(s) 30, 280, 693, 988, 1088, 1094, 1352, 1682
AluI AGCT 8 cut(s) 30, 280, 693, 988, 1088, 1094, 1352, 1682
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 4 cut(s) 743, 1107, 1380, 1568
AlwI GGATC 3 cut(s) 188, 839, 1143
AoxI GGCC 7 cut(s) 228, 238, 299, 388, 420, 1076, 1789
ApeKI GCWGC 3 cut(s) 1305, 1352, 1679
ApoI RAATTY 2 cut(s) 1770, 1919
Asp700I GAANNNNTTC 2 cut(s) 402, 1923
Asp718I GGTACC 1 cut(s) 443
AspLEI GCGC 4 cut(s) 118, 133, 1272, 1310
AspS9I GGNCC 4 cut(s) 229, 982, 1076, 1871
AsuC2I CCSGG 1 cut(s) 1075
AsuHPI GGTGA 4 cut(s) 715, 799, 1198, 1714
AsuNHI GCTAGC 2 cut(s) 1094, 1280
AvaII GGWCC 2 cut(s) 982, 1871
BaeI ACNNNNGTAYC 2 cut(s) 1278, 1311
BalI TGGCCA 2 cut(s) 422, 1791
BanI GGYRCC 4 cut(s) 115, 130, 443, 516
BanII GRGCYC 2 cut(s) 111, 378
BauI CACGAG 1 cut(s) 1742
Bbv12I GWGCWC 1 cut(s) 321
BbvCI CCTCAGC 3 cut(s) 43, 97, 1356
BbvI GCAGC 3 cut(s) 1317, 1339, 1691
BccI CCATC 5 cut(s) 191, 761, 910, 1663, 1852
BceAI ACGGC 3 cut(s) 254, 684, 1703
BcgI CGANNNNNNTGC 2 cut(s) 837, 871
BciT130I CCWGG 6 cut(s) 113, 128, 200, 936, 1556, 1788
BciVI GTATCC 1 cut(s) 558
BclI TGATCA 1 cut(s) 1413
BcnI CCSGG 1 cut(s) 1075
BcoDI GTCTC 4 cut(s) 743, 1107, 1380, 1568
BfaI CTAG 4 cut(s) 623, 989, 1095, 1281
BfmI CTRYAG 3 cut(s) 505, 1533, 1677
BfoI RGCGCY 2 cut(s) 119, 134
BfuI GTATCC 1 cut(s) 558
BglI GCCNNNNNGGC 1 cut(s) 382
BisI GCNGC 6 cut(s) 134, 137, 299, 1306, 1353, 1680
BlsI GCNGC 6 cut(s) 135, 138, 300, 1307, 1354, 1681
Bme1390I CCNGG 7 cut(s) 113, 128, 200, 936, 1075, 1556, 1788
Bme18I GGWCC 2 cut(s) 982, 1871
BmgBI CACGTC 1 cut(s) 1611
BmgT120I GGNCC 4 cut(s) 229, 982, 1076, 1871
BmiI GGNNCC 7 cut(s) 117, 132, 147, 375, 445, 518, 1872
BmrFI CCNGG 7 cut(s) 113, 128, 200, 936, 1075, 1556, 1788
BmsI GCATC 5 cut(s) 674, 835, 1171, 1478, 1827
BmtI GCTAGC 2 cut(s) 1098, 1284
BpmI CTGGAG 1 cut(s) 1368
Bpu10I CCTNAGC 3 cut(s) 43, 97, 1356
BpuEI CTTGAG 1 cut(s) 400
BpuMI CCSGG 1 cut(s) 1075
BsaAI YACGTR 1 cut(s) 1915
BsaBI GATNNNNATC 2 cut(s) 830, 1646
BsaHI GRCGYC 2 cut(s) 116, 131
BsaI GGTCTC 1 cut(s) 743
BsaJI CCNNGG 7 cut(s) 111, 126, 141, 198, 1297, 1555, 1938
BsaWI WCCGGW 3 cut(s) 415, 519, 1808
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bsc4I CCNNNNNNNGG 4 cut(s) 188, 338, 360, 429
Bse118I RCCGGY 1 cut(s) 236
Bse1I ACTGG 2 cut(s) 1036, 1695
Bse3DI GCAATG 1 cut(s) 1455
Bse8I GATNNNNATC 2 cut(s) 830, 1646
BseBI CCWGG 6 cut(s) 113, 128, 200, 936, 1556, 1788
BseDI CCNNGG 7 cut(s) 111, 126, 141, 198, 1297, 1555, 1938
BseGI GGATG 6 cut(s) 921, 1070, 1264, 1418, 1565, 1844
BseJI GATNNNNATC 2 cut(s) 830, 1646
BseLI CCNNNNNNNGG 4 cut(s) 188, 338, 360, 429
BseMI GCAATG 1 cut(s) 1455
BseMII CTCAG 9 cut(s) 33, 57, 111, 326, 1118, 1347, 1391, 1645, 1828
BseNI ACTGG 2 cut(s) 1036, 1695
BseRI GAGGAG 2 cut(s) 526, 935
BseXI GCAGC 3 cut(s) 1317, 1339, 1691
BshFI GGCC 7 cut(s) 230, 240, 301, 390, 422, 1078, 1791
BshNI GGYRCC 4 cut(s) 115, 130, 443, 516
BsiHKAI GWGCWC 1 cut(s) 321
BsiSI CCGG 6 cut(s) 189, 237, 416, 520, 1074, 1809
BslFI GGGAC 1 cut(s) 1173
BslI CCNNNNNNNGG 4 cut(s) 188, 338, 360, 429
BsmAI GTCTC 4 cut(s) 743, 1107, 1380, 1568
BsmBI CGTCTC 1 cut(s) 1107
BsmFI GGGAC 1 cut(s) 1173
BsmI GAATGC 1 cut(s) 1850
BsnI GGCC 7 cut(s) 230, 240, 301, 390, 422, 1078, 1791
Bso31I GGTCTC 1 cut(s) 743
Bsp1286I GDGCHC 3 cut(s) 111, 321, 378
Bsp1407I TGTACA 2 cut(s) 1167, 1605
Bsp143I GATC 6 cut(s) 74, 169, 193, 831, 1135, 1413
BspANI GGCC 7 cut(s) 230, 240, 301, 390, 422, 1078, 1791
BspCNI CTCAG 9 cut(s) 32, 56, 110, 327, 1117, 1348, 1390, 1644, 1827
BspLI GGNNCC 7 cut(s) 117, 132, 147, 375, 445, 518, 1872
BspMAI CTGCAG 1 cut(s) 1681
BspOI GCTAGC 2 cut(s) 1098, 1284
BspPI GGATC 3 cut(s) 188, 839, 1143
BspT107I GGYRCC 4 cut(s) 115, 130, 443, 516
BspTNI GGTCTC 1 cut(s) 743
BsrBI CCGCTC 1 cut(s) 1876
BsrDI GCAATG 1 cut(s) 1455
BsrFI RCCGGY 1 cut(s) 236
BsrGI TGTACA 2 cut(s) 1167, 1605
BsrI ACTGG 2 cut(s) 1036, 1695
BssAI RCCGGY 1 cut(s) 236
BssECI CCNNGG 7 cut(s) 111, 126, 141, 198, 1297, 1555, 1938
BssMI GATC 6 cut(s) 74, 169, 193, 831, 1135, 1413
BssNI GRCGYC 2 cut(s) 116, 131
BssSI CACGAG 1 cut(s) 1742
BssT1I CCWWGG 3 cut(s) 141, 1297, 1938
Bst2BI CACGAG 1 cut(s) 1742
Bst2UI CCWGG 6 cut(s) 113, 128, 200, 936, 1556, 1788
Bst4CI ACNGT 6 cut(s) 403, 670, 956, 976, 1059, 1888
Bst6I CTCTTC 1 cut(s) 870
BstACI GRCGYC 2 cut(s) 116, 131
BstAPI GCANNNNNTGC 2 cut(s) 53, 1693
BstAUI TGTACA 2 cut(s) 1167, 1605
BstBAI YACGTR 1 cut(s) 1915
BstC8I GCNNGC 5 cut(s) 49, 238, 278, 1096, 1282
BstENI CCTNNNNNAGG 1 cut(s) 336
BstF5I GGATG 6 cut(s) 921, 1070, 1264, 1418, 1565, 1844
BstH2I RGCGCY 2 cut(s) 119, 134
BstHHI GCGC 4 cut(s) 118, 133, 1272, 1310
BstKTI GATC 6 cut(s) 77, 172, 196, 834, 1138, 1416
BstMAI GTCTC 4 cut(s) 743, 1107, 1380, 1568
BstMBI GATC 6 cut(s) 74, 169, 193, 831, 1135, 1413
BstMWI GCNNNNNNNGC 8 cut(s) 11, 53, 115, 242, 382, 608, 1544, 1693
BstNI CCWGG 6 cut(s) 113, 128, 200, 936, 1556, 1788
BstNSI RCATGY 2 cut(s) 70, 872
BstSCI CCNGG 7 cut(s) 111, 126, 198, 934, 1073, 1554, 1786
BstSFI CTRYAG 3 cut(s) 505, 1533, 1677
BstV1I GCAGC 3 cut(s) 1317, 1339, 1691
BstXI CCANNNNNNTGG 2 cut(s) 309, 649
BsuI GTATCC 1 cut(s) 558
BsuRI GGCC 7 cut(s) 230, 240, 301, 390, 422, 1078, 1791
BtrI CACGTC 1 cut(s) 1611
BtsCI GGATG 6 cut(s) 921, 1070, 1264, 1418, 1565, 1844
BtsI GCAGTG 2 cut(s) 855, 917
BtsIMutI CAGTG 3 cut(s) 393, 855, 917
Cac8I GCNNGC 5 cut(s) 49, 238, 278, 1096, 1282
CfoI GCGC 4 cut(s) 118, 133, 1272, 1310
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 4 cut(s) 229, 982, 1076, 1871
CseI GACGC 2 cut(s) 1591, 1752
Csp6I GTAC 8 cut(s) 203, 444, 952, 1108, 1168, 1287, 1606, 1912
CspCI CAANNNNNGTGG 2 cut(s) 1603, 1638
CviAII CATG 6 cut(s) 67, 869, 1246, 1318, 1569, 1867
CviQI GTAC 8 cut(s) 203, 444, 952, 1108, 1168, 1287, 1606, 1912
DinI GGCGCC 2 cut(s) 117, 132
DpnI GATC 6 cut(s) 76, 171, 195, 833, 1137, 1415
DpnII GATC 6 cut(s) 74, 169, 193, 831, 1135, 1413
EaeI YGGCCR 3 cut(s) 299, 420, 1789
Eam1104I CTCTTC 1 cut(s) 870
EarI CTCTTC 1 cut(s) 870
EciI GGCGGA 2 cut(s) 148, 1446
Eco130I CCWWGG 3 cut(s) 141, 1297, 1938
Eco24I GRGCYC 2 cut(s) 111, 378
Eco31I GGTCTC 1 cut(s) 743
Eco47I GGWCC 2 cut(s) 982, 1871
EcoNI CCTNNNNNAGG 1 cut(s) 336
EcoRII CCWGG 6 cut(s) 111, 126, 198, 934, 1554, 1786
EcoT14I CCWWGG 3 cut(s) 141, 1297, 1938
EcoT38I GRGCYC 2 cut(s) 111, 378
EgeI GGCGCC 2 cut(s) 117, 132
EheI GGCGCC 2 cut(s) 117, 132
ErhI CCWWGG 3 cut(s) 141, 1297, 1938
Esp3I CGTCTC 1 cut(s) 1107
FaeI CATG 6 cut(s) 70, 872, 1249, 1321, 1572, 1870
FalI AAGNNNNNCTT 2 cut(s) 40, 72
FaqI GGGAC 1 cut(s) 1173
FatI CATG 6 cut(s) 66, 868, 1245, 1317, 1568, 1866
FbaI TGATCA 1 cut(s) 1413
FblI GTMKAC 1 cut(s) 656
Fnu4HI GCNGC 6 cut(s) 134, 137, 299, 1306, 1353, 1680
FokI GGATG 6 cut(s) 928, 1057, 1271, 1405, 1572, 1831
FriOI GRGCYC 2 cut(s) 111, 378
Fsp4HI GCNGC 6 cut(s) 134, 137, 299, 1306, 1353, 1680
FspBI CTAG 4 cut(s) 623, 989, 1095, 1281
GlaI GCGC 4 cut(s) 117, 132, 1271, 1309
GluI GCNGC 6 cut(s) 134, 137, 299, 1306, 1353, 1680
GsuI CTGGAG 1 cut(s) 1368
HaeII RGCGCY 2 cut(s) 119, 134
HaeIII GGCC 7 cut(s) 230, 240, 301, 390, 422, 1078, 1791
HapII CCGG 6 cut(s) 189, 237, 416, 520, 1074, 1809
HgaI GACGC 2 cut(s) 1591, 1752
HhaI GCGC 4 cut(s) 118, 133, 1272, 1310
Hin1I GRCGYC 2 cut(s) 116, 131
Hin1II CATG 6 cut(s) 70, 872, 1249, 1321, 1572, 1870
Hin6I GCGC 4 cut(s) 116, 131, 1270, 1308
HinP1I GCGC 4 cut(s) 116, 131, 1270, 1308
HincII GTYRAC 2 cut(s) 657, 666
HindII GTYRAC 2 cut(s) 657, 666
HinfI GANTC 3 cut(s) 328, 653, 1812
HpaII CCGG 6 cut(s) 189, 237, 416, 520, 1074, 1809
HphI GGTGA 4 cut(s) 715, 799, 1198, 1714
Hpy166II GTNNAC 6 cut(s) 657, 666, 1110, 1170, 1608, 1614
Hpy188III TCNNGA 6 cut(s) 835, 1225, 1347, 1502, 1775, 1928
Hpy8I GTNNAC 6 cut(s) 657, 666, 1110, 1170, 1608, 1614
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 4 cut(s) 723, 1089, 1222, 1694
HpyCH4III ACNGT 6 cut(s) 403, 670, 956, 976, 1059, 1888
HpyCH4IV ACGT 2 cut(s) 1610, 1914
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 53, 115, 242, 382, 608, 1544, 1693
HpySE526I ACGT 2 cut(s) 1610, 1914
Hsp92I GRCGYC 2 cut(s) 116, 131
Hsp92II CATG 6 cut(s) 70, 872, 1249, 1321, 1572, 1870
HspAI GCGC 4 cut(s) 116, 131, 1270, 1308
KasI GGCGCC 2 cut(s) 115, 130
KpnI GGTACC 1 cut(s) 447
KroI GCCGGC 1 cut(s) 236
KroNI GCCGGC 1 cut(s) 238
Ksp22I TGATCA 1 cut(s) 1413
Kzo9I GATC 6 cut(s) 74, 169, 193, 831, 1135, 1413
LmnI GCTCC 4 cut(s) 35, 373, 1349, 1760
Lsp1109I GCAGC 3 cut(s) 1317, 1339, 1691
LweI GCATC 5 cut(s) 674, 835, 1171, 1478, 1827
MaeI CTAG 4 cut(s) 623, 989, 1095, 1281
MaeII ACGT 2 cut(s) 1610, 1914
MaeIII GTNAC 4 cut(s) 805, 864, 1435, 1720
MalI GATC 6 cut(s) 76, 171, 195, 833, 1137, 1415
MbiI CCGCTC 1 cut(s) 1876
MboI GATC 6 cut(s) 74, 169, 193, 831, 1135, 1413
MboII GAAGA 3 cut(s) 887, 907, 1384
MfeI CAATTG 2 cut(s) 762, 1185
MhlI GDGCHC 3 cut(s) 111, 321, 378
MlsI TGGCCA 2 cut(s) 422, 1791
MluNI TGGCCA 2 cut(s) 422, 1791
Mly113I GGCGCC 2 cut(s) 116, 131
MlyI GAGTC 2 cut(s) 662, 1806
MmeI TCCRAC 3 cut(s) 351, 726, 960
Mox20I TGGCCA 2 cut(s) 422, 1791
MroNI GCCGGC 1 cut(s) 236
MroXI GAANNNNTTC 2 cut(s) 402, 1923
MscI TGGCCA 2 cut(s) 422, 1791
MseI TTAA 5 cut(s) 542, 906, 1038, 1382, 1902
MslI CAYNNNNRTG 2 cut(s) 224, 522
Msp20I TGGCCA 2 cut(s) 422, 1791
MspI CCGG 6 cut(s) 189, 237, 416, 520, 1074, 1809
MspR9I CCNGG 7 cut(s) 113, 128, 200, 936, 1075, 1556, 1788
MunI CAATTG 2 cut(s) 762, 1185
Mva1269I GAATGC 1 cut(s) 1850
MvaI CCWGG 6 cut(s) 113, 128, 200, 936, 1556, 1788
MwoI GCNNNNNNNGC 8 cut(s) 11, 53, 115, 242, 382, 608, 1544, 1693
NaeI GCCGGC 1 cut(s) 238
NarI GGCGCC 2 cut(s) 116, 131
NciI CCSGG 1 cut(s) 1075
NdeII GATC 6 cut(s) 74, 169, 193, 831, 1135, 1413
NgoMIV GCCGGC 1 cut(s) 236
NheI GCTAGC 2 cut(s) 1094, 1280
NlaIII CATG 6 cut(s) 70, 872, 1249, 1321, 1572, 1870
NlaIV GGNNCC 7 cut(s) 117, 132, 147, 375, 445, 518, 1872
NmeAIII GCCGAG 1 cut(s) 1564
NmuCI GTSAC 3 cut(s) 805, 1435, 1720
NspI RCATGY 2 cut(s) 70, 872
OliI CACNNNNGTG 1 cut(s) 522
PciI ACATGT 2 cut(s) 66, 868
PctI GAATGC 1 cut(s) 1850
PdiI GCCGGC 1 cut(s) 238
PdmI GAANNNNTTC 2 cut(s) 402, 1923
PfeI GAWTC 1 cut(s) 328
PflFI GACNNNGTC 1 cut(s) 661
PkrI GCNGC 6 cut(s) 135, 138, 300, 1307, 1354, 1681
PleI GAGTC 2 cut(s) 661, 1806
PluTI GGCGCC 2 cut(s) 119, 134
PpsI GAGTC 2 cut(s) 661, 1806
Ppu21I YACGTR 1 cut(s) 1915
PscI ACATGT 2 cut(s) 66, 868
Psp6I CCWGG 6 cut(s) 111, 126, 198, 934, 1554, 1786
PspGI CCWGG 6 cut(s) 111, 126, 198, 934, 1554, 1786
PspN4I GGNNCC 7 cut(s) 117, 132, 147, 375, 445, 518, 1872
PspPI GGNCC 4 cut(s) 229, 982, 1076, 1871
PstI CTGCAG 1 cut(s) 1681
PsyI GACNNNGTC 1 cut(s) 661
RsaI GTAC 8 cut(s) 204, 445, 953, 1109, 1169, 1288, 1607, 1913
RsaNI GTAC 8 cut(s) 203, 444, 952, 1108, 1168, 1287, 1606, 1912
RseI CAYNNNNRTG 2 cut(s) 224, 522
SalI GTCGAC 1 cut(s) 655
SaqAI TTAA 5 cut(s) 542, 906, 1038, 1382, 1902
SatI GCNGC 6 cut(s) 134, 137, 299, 1306, 1353, 1680
Sau3AI GATC 6 cut(s) 74, 169, 193, 831, 1135, 1413
Sau96I GGNCC 4 cut(s) 229, 982, 1076, 1871
SchI GAGTC 2 cut(s) 662, 1806
ScrFI CCNGG 7 cut(s) 113, 128, 200, 936, 1075, 1556, 1788
SduI GDGCHC 3 cut(s) 111, 321, 378
SfaNI GCATC 5 cut(s) 674, 835, 1171, 1478, 1827
SfcI CTRYAG 3 cut(s) 505, 1533, 1677
SfoI GGCGCC 2 cut(s) 117, 132
SinI GGWCC 2 cut(s) 982, 1871
SmiMI CAYNNNNRTG 2 cut(s) 224, 522
SmlI CTYRAG 1 cut(s) 379
SmoI CTYRAG 1 cut(s) 379
SspDI GGCGCC 2 cut(s) 115, 130
SspMI CTAG 4 cut(s) 623, 989, 1095, 1281
StyD4I CCNGG 7 cut(s) 111, 126, 198, 934, 1073, 1554, 1786
StyI CCWWGG 3 cut(s) 141, 1297, 1938
TaaI ACNGT 6 cut(s) 403, 670, 956, 976, 1059, 1888
TaiI ACGT 2 cut(s) 1613, 1917
TaqI TCGA 6 cut(s) 326, 453, 656, 834, 1224, 1854
TatI WGTACW 3 cut(s) 1107, 1167, 1605
TauI GCSGC 3 cut(s) 136, 139, 301
TfiI GAWTC 1 cut(s) 328
Tru1I TTAA 5 cut(s) 542, 906, 1038, 1382, 1902
Tru9I TTAA 5 cut(s) 542, 906, 1038, 1382, 1902
TscAI CASTG 3 cut(s) 400, 855, 917
TseFI GTSAC 3 cut(s) 805, 1435, 1720
TseI GCWGC 3 cut(s) 1305, 1352, 1679
Tsp45I GTSAC 3 cut(s) 805, 1435, 1720
TspDTI ATGAA 5 cut(s) 247, 807, 984, 1059, 1727
TspGWI ACGGA 3 cut(s) 905, 1720, 1773
TspRI CASTG 3 cut(s) 400, 855, 917
Tth111I GACNNNGTC 1 cut(s) 661
VpaK11BI GGWCC 2 cut(s) 982, 1871
XagI CCTNNNNNAGG 1 cut(s) 336
XapI RAATTY 2 cut(s) 1770, 1919
XceI RCATGY 2 cut(s) 70, 872
XcmI CCANNNNNNNNNTGG 1 cut(s) 1252
XmiI GTMKAC 1 cut(s) 656
XmnI GAANNNNTTC 2 cut(s) 402, 1923
XspI CTAG 4 cut(s) 623, 989, 1095, 1281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.