Rroxscaffold_3G00229540

AI-2E family transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004461
Physical Location & Seq
Forward (+)
14043505 .. 14050277
6773 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00229540.1

Sequence Viewer

Length: 603 bp
ATGAGTATGCTTCCAATTTCAAAGTCTGCAAGGGTTAGATGCGTTGAAGTTCTTGATAATGCCATATCCGGTGTTCTTTTGGCTACGGATATACCTAGTCATAGTGAATATCTCACGGGACTTAGCATCATTGATGGAATGACATTGTTTCCATCTGCATTGGAGGGTGCAATTATGGGCCTGCTCATAACCACGGTTGTGATTGCTCTCAAGGATTTATACGTGGAATTTGTTCTGGATGAACCCAAGGTTAAAGATGAGTGTTTGATACTTGGCAGCAGTGGAGAATTTTGGAGGAATTTGCTGCTGAAACGATTGGGTAAATTTCGTACCCTTGTTGGTCGATATGGGTATTACCGTATTCTTGAAGCATTGCGATTGCAGGTAGAACTCCAAAAGCGGCTTCATGAACAACTTGAGCAAGACATGTCGGACAAGAACAGTAGTAACAACAAGAAAACTAACAACAAAAAGCTAGTGCTCGAACATCATAAGCTAAACCTCATATTGGAGATAGTGGTTTATCTTGCTCAGCTGTATGATCTAAAGGTCCCGATGACTGAAAAGTCCACTGTAATTCTTGAAATCACCATACTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AI-2E_transport

Protein Analysis

200

Amino Acids

22.67

Weight (kDa)

6.45

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_CC_LHEQLE PF14379 121 - 140 1.7e-06 MYB-CC type transfactor, LHEQLE motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 565
Acc36I ACCTGC 1 cut(s) 373
AciI CCGC 1 cut(s) 400
AcsI RAATTY 4 cut(s) 227, 287, 298, 323
AfaI GTAC 1 cut(s) 331
AfiI CCNNNNNNNGG 1 cut(s) 508
AflIII ACRYGT 1 cut(s) 426
AgsI TTSAA 4 cut(s) 21, 47, 368, 584
AjuI GAANNNNNNNTTGG 2 cut(s) 387, 419
AleI CACNNNNGTG 1 cut(s) 197
AluBI AGCT 3 cut(s) 475, 496, 535
AluI AGCT 3 cut(s) 475, 496, 535
Alw21I GWGCWC 1 cut(s) 483
AoxI GGCC 1 cut(s) 178
ApeKI GCWGC 2 cut(s) 276, 304
ApoI RAATTY 4 cut(s) 227, 287, 298, 323
Asp700I GAANNNNTTC 1 cut(s) 231
AspS9I GGNCC 2 cut(s) 178, 550
AsuHPI GGTGA 1 cut(s) 580
AvaII GGWCC 1 cut(s) 550
Bbv12I GWGCWC 1 cut(s) 483
BbvI GCAGC 2 cut(s) 288, 291
BccI CCATC 2 cut(s) 128, 160
BfaI CTAG 2 cut(s) 96, 476
BfuAI ACCTGC 1 cut(s) 373
BisI GCNGC 3 cut(s) 277, 305, 401
BlpI GCTNAGC 1 cut(s) 531
BlsI GCNGC 3 cut(s) 278, 306, 402
Bme18I GGWCC 1 cut(s) 550
BmgT120I GGNCC 2 cut(s) 178, 550
BmiI GGNNCC 1 cut(s) 552
BmsI GCATC 2 cut(s) 29, 135
Bpu1102I GCTNAGC 1 cut(s) 531
BpuEI CTTGAG 2 cut(s) 194, 437
BsaAI YACGTR 1 cut(s) 223
BsaJI CCNNGG 2 cut(s) 192, 246
BsaWI WCCGGW 1 cut(s) 68
Bsc4I CCNNNNNNNGG 1 cut(s) 508
Bse3DI GCAATG 1 cut(s) 371
BseDI CCNNGG 2 cut(s) 192, 246
BseGI GGATG 1 cut(s) 244
BseLI CCNNNNNNNGG 1 cut(s) 508
BseMI GCAATG 1 cut(s) 371
BseMII CTCAG 1 cut(s) 545
BseXI GCAGC 2 cut(s) 288, 291
BshFI GGCC 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 483
BsiSI CCGG 1 cut(s) 69
BslFI GGGAC 2 cut(s) 132, 536
BslI CCNNNNNNNGG 1 cut(s) 508
BsmFI GGGAC 2 cut(s) 132, 536
BsnI GGCC 1 cut(s) 180
Bsp1286I GDGCHC 1 cut(s) 483
Bsp143I GATC 1 cut(s) 541
Bsp1720I GCTNAGC 1 cut(s) 531
BspACI CCGC 1 cut(s) 400
BspANI GGCC 1 cut(s) 180
BspCNI CTCAG 1 cut(s) 544
BspHI TCATGA 1 cut(s) 406
BspLI GGNNCC 1 cut(s) 552
BspMI ACCTGC 1 cut(s) 373
BsrDI GCAATG 1 cut(s) 371
BssECI CCNNGG 2 cut(s) 192, 246
BssMI GATC 1 cut(s) 541
BssT1I CCWWGG 1 cut(s) 246
Bst4CI ACNGT 4 cut(s) 196, 359, 443, 574
BstBAI YACGTR 1 cut(s) 223
BstC8I GCNNGC 1 cut(s) 182
BstDEI CTNAG 2 cut(s) 122, 531
BstDSI CCRYGG 1 cut(s) 192
BstF5I GGATG 1 cut(s) 244
BstKTI GATC 1 cut(s) 544
BstMBI GATC 1 cut(s) 541
BstNSI RCATGY 1 cut(s) 430
BstV1I GCAGC 2 cut(s) 288, 291
BsuRI GGCC 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 192
BtsCI GGATG 1 cut(s) 244
BtsI GCAGTG 1 cut(s) 286
BtsIMutI CAGTG 2 cut(s) 286, 570
BveI ACCTGC 1 cut(s) 373
Cac8I GCNNGC 1 cut(s) 182
CciI TCATGA 1 cut(s) 406
Cfr13I GGNCC 2 cut(s) 178, 550
Csp6I GTAC 1 cut(s) 330
CviAII CATG 2 cut(s) 407, 427
CviJI RGCY 6 cut(s) 83, 180, 403, 475, 496, 535
CviKI_1 RGCY 6 cut(s) 83, 180, 403, 475, 496, 535
CviQI GTAC 1 cut(s) 330
DdeI CTNAG 2 cut(s) 122, 531
DpnI GATC 1 cut(s) 543
DpnII GATC 1 cut(s) 541
DrdI GACNNNNNNGTC 1 cut(s) 565
DseDI GACNNNNNNGTC 1 cut(s) 565
Eco130I CCWWGG 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 550
EcoO109I RGGNCCY 1 cut(s) 550
EcoT14I CCWWGG 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 2 cut(s) 410, 430
FaqI GGGAC 2 cut(s) 132, 536
FatI CATG 2 cut(s) 406, 426
Fnu4HI GCNGC 3 cut(s) 277, 305, 401
FokI GGATG 1 cut(s) 251
Fsp4HI GCNGC 3 cut(s) 277, 305, 401
FspBI CTAG 2 cut(s) 96, 476
GluI GCNGC 3 cut(s) 277, 305, 401
HaeIII GGCC 1 cut(s) 180
HapII CCGG 1 cut(s) 69
Hin1II CATG 2 cut(s) 410, 430
HpaII CCGG 1 cut(s) 69
HphI GGTGA 1 cut(s) 580
Hpy166II GTNNAC 1 cut(s) 570
Hpy188I TCNGA 1 cut(s) 433
Hpy188III TCNNGA 6 cut(s) 53, 236, 365, 407, 553, 581
Hpy8I GTNNAC 1 cut(s) 570
HpyCH4III ACNGT 4 cut(s) 196, 359, 443, 574
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 4 cut(s) 29, 158, 170, 382
HpyF3I CTNAG 2 cut(s) 122, 531
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 2 cut(s) 410, 430
Kzo9I GATC 1 cut(s) 541
LpnPI CCDG 4 cut(s) 82, 194, 221, 368
Lsp1109I GCAGC 2 cut(s) 288, 291
LweI GCATC 2 cut(s) 29, 135
MaeI CTAG 2 cut(s) 96, 476
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 446
MalI GATC 1 cut(s) 543
MboI GATC 1 cut(s) 541
MhlI GDGCHC 1 cut(s) 483
MluCI AATT 7 cut(s) 15, 171, 227, 287, 298, 323, 576
MmeI TCCRAC 1 cut(s) 411
MnlI CCTC 3 cut(s) 157, 288, 512
MroXI GAANNNNTTC 1 cut(s) 231
MseI TTAA 1 cut(s) 252
MslI CAYNNNNRTG 1 cut(s) 197
MspA1I CMGCKG 1 cut(s) 535
MspI CCGG 1 cut(s) 69
NdeII GATC 1 cut(s) 541
NlaIII CATG 2 cut(s) 410, 430
NlaIV GGNNCC 1 cut(s) 552
NspI RCATGY 1 cut(s) 430
OliI CACNNNNGTG 1 cut(s) 197
PagI TCATGA 1 cut(s) 406
PciI ACATGT 1 cut(s) 426
PdmI GAANNNNTTC 1 cut(s) 231
PkrI GCNGC 3 cut(s) 278, 306, 402
Ppu21I YACGTR 1 cut(s) 223
PpuMI RGGWCCY 1 cut(s) 550
PscI ACATGT 1 cut(s) 426
Psp5II RGGWCCY 1 cut(s) 550
PspN4I GGNNCC 1 cut(s) 552
PspPI GGNCC 2 cut(s) 178, 550
PspPPI RGGWCCY 1 cut(s) 550
PvuII CAGCTG 1 cut(s) 535
RsaI GTAC 1 cut(s) 331
RsaNI GTAC 1 cut(s) 330
RseI CAYNNNNRTG 1 cut(s) 197
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 3 cut(s) 277, 305, 401
Sau3AI GATC 1 cut(s) 541
Sau96I GGNCC 2 cut(s) 178, 550
SduI GDGCHC 1 cut(s) 483
SetI ASST 9 cut(s) 97, 225, 252, 387, 477, 498, 504, 537, 552
SfaNI GCATC 2 cut(s) 29, 135
SinI GGWCC 1 cut(s) 550
SmiMI CAYNNNNRTG 1 cut(s) 197
SmlI CTYRAG 3 cut(s) 209, 416, 596
SmoI CTYRAG 3 cut(s) 209, 416, 596
Sse9I AATT 7 cut(s) 15, 171, 227, 287, 298, 323, 576
SsiI CCGC 1 cut(s) 400
SspMI CTAG 2 cut(s) 96, 476
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 4 cut(s) 196, 359, 443, 574
TaiI ACGT 1 cut(s) 225
TaqI TCGA 2 cut(s) 343, 483
TasI AATT 7 cut(s) 15, 171, 227, 287, 298, 323, 576
TauI GCSGC 1 cut(s) 403
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 2 cut(s) 286, 577
TseI GCWGC 2 cut(s) 276, 304
TspDTI ATGAA 3 cut(s) 255, 395, 423
TspGWI ACGGA 1 cut(s) 101
TspRI CASTG 2 cut(s) 286, 577
VpaK11BI GGWCC 1 cut(s) 550
XapI RAATTY 4 cut(s) 227, 287, 298, 323
XceI RCATGY 1 cut(s) 430
XmnI GAANNNNTTC 1 cut(s) 231
XspI CTAG 2 cut(s) 96, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.