MD11G1118400.v1.1

respiratory burst oxidase homolog protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010309
Physical Location & Seq
Forward (+)
10784434 .. 10788882
4449 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1118400.v1.1.491

Sequence Viewer

Length: 2580 bp
ATGGTGGCAATGAACATGTCAAAGGATGATGACCAGAACGATCCCAACAAATGGAAGCTTGAAACAATTGATATTGACCCCATGGTGGATATTCCCTTAAGTAACAATGACACAGAAGGCAATGTGACCAAGAGTTCCAACGATGCGACGTTGAAGCGGAACAACAGCAGTGTAAGGAAGAGAAATGGGGTTCAGGGAAACAATCATGGCAGCGTGGGATGTAGGGCGGAACCAGGAGGGAAAGAAGGGATTAAGAGCTTGCGTTTTCTGGATAGGACAGCCACAGGAAAAGAAGTGGATGCATGGAAGCAGGTTGAGAAGCGGTTTAATCAATACGCGGTCGCTGATAGGCTCTCCAGGGACAAATTTGGAGCCTGCATTGGGATGGGAGGAGAATCGAAGGAATTTGCAGGCGAATTGTTTGATGCATTGGCTAGGAGAAGGATAATATGCGAGAAAGGTGGGATCACTCTTGAAGAATTAAGGTTGTTTTGGGAAGACATAACTAATGAAGACATGGAAGCAAGACTTCAGATATTCTTCGACATATGTGACAAGAATGGTGATGGGAAGCTCTCCGAGGATGAGGTAGAAGAGGTTATAGTTTTGAGCGCCTCCGCAAACAAGCTGGCAAATCTTAAAATGCAAGCAAAATCATACGCAGCTTTGATCATGGAAGAGATTGACCCTGACCACCTTGGATATATAGAGATGTGGCAGTTGGAAACCCTACTGAGGGGAATGGCAGTGTCCGAAGATAACCAAAAACTCGGAAAGAGAACCCAATCTCTCACGAAATCCATGATTCCCAGAAGTTACAGAACTCCCATTAGCAAATTCCTGTCTACAACTGCAGAACTTGTGCAAGAAAACTGGAAGAAAATATGGATTGTGATATTGTGGTTAGCTATCAACATAGGCCTCTTCACTTGGAAATACCGCCAATACCAGAAGCATGGATTATATAAACTCATGGGTACATGCCTCTGCATTGCAAAGGGCTCTGCTGAGACTCTCAAGTTCAATATGGCTCTCATTCTCATTCCAGTTTGTAGGGGAACTCTTACCGCAATTCGATCATCATTTCTCAGCAACCTAATCCCTTTTGATGACAACATCAAATTCCATAAGTTGATTGCACTGGGAATTGTAATTTCGACTTCTCTACATGCTGGTTTACACATAACTTGTGATTTCCCAAGATTGATAACATCCCAGGAAGATGAGTTCATGGATGTTGCCGGGACATATTTCAATTATAAGCAGCCAACTTACATGGACTTGGTTAATAGTATTCCAGGAATAACTGGGATTCTGATGACGGTTCTGATGATGTTTTCGTTCACACTGGCATTAAATGTAATGAGAAAAAATGTCAACTTACCGTGGCCACTCAACATATTGTCAGGGTTCAATTCTTTCTGGTATGCACATCATTTGCTTGTGTTGGTGTATATGCTCTTTGTCATGCATGGTTACTTCCTAATTTTCACGAGCGACTGGCACAAGAAGACGACATGGATGTATATCCTTGTTCCAGTAATGTTGTATGCAATTGAGCGTTTTATTATAATCATAAAAGAAATTAATCTCCCTGTCCATGTCATTAAGGCGGTAATATATACGGGAAATGTTCTGGCTTTGTACATGAGCAAACCTCATGGATTCAAGTATAAAAGTGGAATGTACCTCTTTCTTAAGTGCCCTGATATATCAGGTTTTGAATGGCATCCCTTCACCATCACTTCTGCCCCAGGAGATGACTACTTGAGCGTCCACATACGAAGCTTGGGAGACTGGACTACGGAGCTTAGAGAAAGATTCGAAAAGGCGTGCAGCCAGCTTCAAAGTACACAAACTAGAAAGGGAAATCTCGTTAGAATAGAAACGAAAGCGCATTCAAACTATGGTCATGAAATAGAAGGATATCCGAAGATCGTCATAAAGGGACCATATGGTGCACCGGCTCAAAATTACATAAAGTTTGATATCTTATTGCTCATAGGTCTGGGAATTGGAGCAACTCCCTTCGTCAGCATCATAAAAGATATCATAAACCAAATAAACCTCAACTTGGCTTATAATCCTGATTCGATAACTGAGCATCAAGAAACATCCGTGGAAACCAAATCAAACAACATGCAAAGGGAGGCATATAAAAGGGGTCCAGAAAGAGCATACTTTTATTGGATAACAAGGGAGCAAGCTTCCTTTGAATGGTTTAAAGGTGTCATGGACGACATTGCAGAATGTGATCACTATCATATGATAGAAATGCACAACTACTTGACCAGCGTGTACGAAGAAGGAGATGCGAGGTCTGCTCTTATTGCCATGGTGCAGAAACTGCAACATGCTAAGAATGGAGTTGATGTTGTCTCAGAAAGCCGGATAAGAACTCATTTTGCAAGGCCTAATTGGAGAAAGGTGTTTTCCGACTTGGCTGCCGCACATGAATCCTCCCGAATAGGTGTCTTCTATTGTGGAAGTCCAACACTTACCAAAACACTCAGGGAGCTATGCCTAGAATTTAGTCTAAACTCAGCAACTCGGTTCGTCTTCCACAAGGAGAACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04624 map04013 map04624
Pfam Domains
Protein Families
EF-hand_6 FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

860

Amino Acids

98.11

Weight (kDa)

7.59

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADPH_Ox PF08414 75 - 174 8.1e-30 Respiratory burst NADPH oxidase
Ferric_reduct PF01794 345 - 489 1.9e-11 Ferric reductase like transmembrane component
FAD_binding_8 PF08022 533 - 656 1.8e-28 FAD-binding domain
NAD_binding_6 PF08030 662 - 841 1.2e-45 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1260, 1571, 2082
Acc36I ACCTGC 1 cut(s) 301
AccB7I CCANNNNNTGG 1 cut(s) 51
AccI GTMKAC 1 cut(s) 845
AccII CGCG 1 cut(s) 338
AciI CCGC 9 cut(s) 157, 227, 322, 338, 618, 940, 1068, 1613, 2448
AclWI GGATC 2 cut(s) 35, 473
AcoI YGGCCR 1 cut(s) 1388
AcsI RAATTY 5 cut(s) 365, 404, 836, 1121, 2528
AcuI CTGAAG 1 cut(s) 515
AfaI GTAC 5 cut(s) 979, 1646, 1688, 1852, 2300
AfiI CCNNNNNNNGG 7 cut(s) 51, 85, 381, 735, 736, 2074, 2217
AflII CTTAAG 2 cut(s) 97, 1697
AflIII ACRYGT 1 cut(s) 15
AjnI CCWGG 5 cut(s) 232, 356, 1215, 1297, 1753
AloI GAACNNNNNNTCC 2 cut(s) 1211, 1243
Alw21I GWGCWC 1 cut(s) 1963
Alw26I GTCTC 3 cut(s) 1004, 1788, 2383
Alw44I GTGCAC 1 cut(s) 1959
AlwI GGATC 2 cut(s) 35, 473
AlwNI CAGNNNCTG 1 cut(s) 2347
AoxI GGCC 3 cut(s) 919, 1388, 2411
ApaLI GTGCAC 1 cut(s) 1959
ApeKI GCWGC 5 cut(s) 210, 662, 1264, 1836, 2444
ApoI RAATTY 5 cut(s) 365, 404, 836, 1121, 2528
AseI ATTAAT 1 cut(s) 1587
AspLEI GCGC 2 cut(s) 614, 1897
AspS9I GGNCC 2 cut(s) 1949, 2165
AsuC2I CCSGG 1 cut(s) 1243
AsuHPI GGTGA 2 cut(s) 575, 1729
AsuII TTCGAA 1 cut(s) 1824
AvaII GGWCC 2 cut(s) 1949, 2165
BaeGI GKGCMC 2 cut(s) 1706, 1963
BalI TGGCCA 1 cut(s) 1390
BanII GRGCYC 1 cut(s) 1004
BauI CACGAG 1 cut(s) 1492
BbsI GAAGAC 5 cut(s) 504, 519, 1517, 2467, 2551
Bbv12I GWGCWC 1 cut(s) 1963
BbvI GCAGC 5 cut(s) 222, 674, 1276, 1848, 2431
BccI CCATC 3 cut(s) 379, 560, 1748
BcgI CGANNNNNNTGC 2 cut(s) 2426, 2460
BciT130I CCWGG 5 cut(s) 234, 358, 1217, 1299, 1755
BclI TGATCA 2 cut(s) 669, 2254
BcnI CCSGG 1 cut(s) 1243
BcoDI GTCTC 3 cut(s) 1004, 1788, 2383
BfaI CTAG 4 cut(s) 435, 1860, 2525, 2578
BfmI CTRYAG 1 cut(s) 852
BfoI RGCGCY 1 cut(s) 615
BfrI CTTAAG 2 cut(s) 97, 1697
BfuAI ACCTGC 1 cut(s) 301
BisI GCNGC 6 cut(s) 211, 663, 1265, 1837, 2445, 2448
BlsI GCNGC 6 cut(s) 212, 664, 1266, 1838, 2446, 2449
Bme1390I CCNGG 6 cut(s) 234, 358, 1217, 1243, 1299, 1755
Bme18I GGWCC 2 cut(s) 1949, 2165
BmgT120I GGNCC 2 cut(s) 1949, 2165
BmiI GGNNCC 4 cut(s) 231, 373, 1950, 2166
BmrFI CCNGG 6 cut(s) 234, 358, 1217, 1243, 1299, 1755
BmrI ACTGGG 2 cut(s) 1151, 1317
BmsI GCATC 7 cut(s) 133, 289, 415, 1738, 2046, 2113, 2302
BmuI ACTGGG 2 cut(s) 1151, 1317
BpiI GAAGAC 5 cut(s) 504, 519, 1517, 2467, 2551
BplI GAGNNNNNCTC 4 cut(s) 1642, 1674, 2308, 2340
BpmI CTGGAG 1 cut(s) 340
Bpu14I TTCGAA 1 cut(s) 1824
BpuEI CTTGAG 2 cut(s) 1001, 1789
BpuMI CCSGG 1 cut(s) 1243
BsaBI GATNNNNATC 2 cut(s) 1526, 2259
BsaJI CCNNGG 9 cut(s) 81, 357, 579, 697, 1215, 1385, 1753, 2118, 2334
BsaXI ACNNNNNCTCC 2 cut(s) 2358, 2388
Bsc4I CCNNNNNNNGG 7 cut(s) 51, 85, 381, 735, 736, 2074, 2217
Bse118I RCCGGY 1 cut(s) 1963
Bse1I ACTGG 8 cut(s) 878, 1046, 1146, 1312, 1353, 1505, 1538, 1802
Bse3DI GCAATG 4 cut(s) 15, 127, 990, 2241
Bse8I GATNNNNATC 2 cut(s) 1526, 2259
BseBI CCWGG 5 cut(s) 234, 358, 1217, 1299, 1755
BseDI CCNNGG 9 cut(s) 81, 357, 579, 697, 1215, 1385, 1753, 2118, 2334
BseJI GATNNNNATC 2 cut(s) 1526, 2259
BseLI CCNNNNNNNGG 7 cut(s) 51, 85, 381, 735, 736, 2074, 2217
BseMI GCAATG 4 cut(s) 15, 127, 990, 2241
BseMII CTCAG 7 cut(s) 725, 999, 1102, 2091, 2394, 2524, 2556
BseNI ACTGG 8 cut(s) 878, 1046, 1146, 1312, 1353, 1505, 1538, 1802
BseRI GAGGAG 1 cut(s) 405
BseSI GKGCMC 2 cut(s) 1706, 1963
BseXI GCAGC 5 cut(s) 222, 674, 1276, 1848, 2431
BsgI GTGCAG 2 cut(s) 1855, 2360
Bsh1236I CGCG 1 cut(s) 338
Bsh1285I CGRYCG 1 cut(s) 342
BshFI GGCC 3 cut(s) 921, 1390, 2413
BsiEI CGRYCG 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 1963
BsiSI CCGG 3 cut(s) 1242, 1964, 2389
BslFI GGGAC 3 cut(s) 374, 1258, 1962
BslI CCNNNNNNNGG 7 cut(s) 51, 85, 381, 735, 736, 2074, 2217
BsmAI GTCTC 3 cut(s) 1004, 1788, 2383
BsmFI GGGAC 3 cut(s) 374, 1258, 1962
BsmI GAATGC 1 cut(s) 1897
BsnI GGCC 3 cut(s) 921, 1390, 2413
Bsp119I TTCGAA 1 cut(s) 1824
Bsp1286I GDGCHC 3 cut(s) 1004, 1706, 1963
Bsp1407I TGTACA 1 cut(s) 1644
Bsp143I GATC 6 cut(s) 40, 465, 669, 1076, 1935, 2254
Bsp19I CCATGG 2 cut(s) 81, 2334
BspACI CCGC 9 cut(s) 157, 227, 322, 338, 618, 940, 1068, 1613, 2448
BspANI GGCC 3 cut(s) 921, 1390, 2413
BspCNI CTCAG 7 cut(s) 726, 1000, 1101, 2092, 2393, 2523, 2555
BspFNI CGCG 1 cut(s) 338
BspHI TCATGA 1 cut(s) 1912
BspLI GGNNCC 4 cut(s) 231, 373, 1950, 2166
BspMAI CTGCAG 1 cut(s) 856
BspMI ACCTGC 1 cut(s) 301
BspPI GGATC 2 cut(s) 35, 473
BspT104I TTCGAA 1 cut(s) 1824
BspTI CTTAAG 2 cut(s) 97, 1697
BsrDI GCAATG 4 cut(s) 15, 127, 990, 2241
BsrFI RCCGGY 1 cut(s) 1963
BsrGI TGTACA 1 cut(s) 1644
BsrI ACTGG 8 cut(s) 878, 1046, 1146, 1312, 1353, 1505, 1538, 1802
BssAI RCCGGY 1 cut(s) 1963
BssECI CCNNGG 9 cut(s) 81, 357, 579, 697, 1215, 1385, 1753, 2118, 2334
BssMI GATC 6 cut(s) 40, 465, 669, 1076, 1935, 2254
BssSI CACGAG 1 cut(s) 1492
BssT1I CCWWGG 3 cut(s) 81, 697, 2334
Bst2BI CACGAG 1 cut(s) 1492
Bst2UI CCWGG 5 cut(s) 234, 358, 1217, 1299, 1755
Bst4CI ACNGT 2 cut(s) 1324, 1386
Bst6I CTCTTC 4 cut(s) 173, 588, 672, 929
BstAFI CTTAAG 2 cut(s) 97, 1697
BstAPI GCANNNNNTGC 1 cut(s) 2347
BstAUI TGTACA 1 cut(s) 1644
BstBI TTCGAA 1 cut(s) 1824
BstC8I GCNNGC 8 cut(s) 260, 376, 412, 630, 648, 1834, 1841, 2205
BstDEI CTNAG 9 cut(s) 734, 1008, 1088, 1811, 2100, 2358, 2380, 2510, 2542
BstDSI CCRYGG 4 cut(s) 81, 1385, 2118, 2334
BstFNI CGCG 1 cut(s) 338
BstH2I RGCGCY 1 cut(s) 615
BstHHI GCGC 2 cut(s) 614, 1897
BstKTI GATC 6 cut(s) 43, 468, 672, 1079, 1938, 2257
BstMAI GTCTC 3 cut(s) 1004, 1788, 2383
BstMBI GATC 6 cut(s) 40, 465, 669, 1076, 1935, 2254
BstMCI CGRYCG 1 cut(s) 342
BstMWI GCNNNNNNNGC 3 cut(s) 2321, 2330, 2347
BstNI CCWGG 5 cut(s) 234, 358, 1217, 1299, 1755
BstNSI RCATGY 5 cut(s) 19, 984, 1172, 2143, 2357
BstSCI CCNGG 6 cut(s) 232, 356, 1215, 1241, 1297, 1753
BstSFI CTRYAG 1 cut(s) 852
BstSLI GKGCMC 2 cut(s) 1706, 1963
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 5 cut(s) 222, 674, 1276, 1848, 2431
BstV2I GAAGAC 5 cut(s) 504, 519, 1517, 2467, 2551
BstXI CCANNNNNNTGG 1 cut(s) 956
BsuRI GGCC 3 cut(s) 921, 1390, 2413
BtgI CCRYGG 4 cut(s) 81, 1385, 2118, 2334
BtsI GCAGTG 2 cut(s) 175, 753
BtsIMutI CAGTG 4 cut(s) 175, 753, 1139, 1346
BveI ACCTGC 1 cut(s) 301
Cac8I GCNNGC 8 cut(s) 260, 376, 412, 630, 648, 1834, 1841, 2205
CaiI CAGNNNCTG 1 cut(s) 2347
CciI TCATGA 1 cut(s) 1912
CfoI GCGC 2 cut(s) 614, 1897
Cfr10I RCCGGY 1 cut(s) 1963
Cfr13I GGNCC 2 cut(s) 1949, 2165
CseI GACGC 1 cut(s) 1762
Csp6I GTAC 5 cut(s) 978, 1645, 1687, 1851, 2299
CviQI GTAC 5 cut(s) 978, 1645, 1687, 1851, 2299
DdeI CTNAG 9 cut(s) 734, 1008, 1088, 1811, 2100, 2358, 2380, 2510, 2542
DpnI GATC 6 cut(s) 42, 467, 671, 1078, 1937, 2256
DpnII GATC 6 cut(s) 40, 465, 669, 1076, 1935, 2254
DraI TTTAAA 1 cut(s) 2224
EaeI YGGCCR 1 cut(s) 1388
Eam1104I CTCTTC 4 cut(s) 173, 588, 672, 929
EarI CTCTTC 4 cut(s) 173, 588, 672, 929
EciI GGCGGA 1 cut(s) 242
Eco130I CCWWGG 3 cut(s) 81, 697, 2334
Eco147I AGGCCT 2 cut(s) 921, 2413
Eco24I GRGCYC 1 cut(s) 1004
Eco32I GATATC 3 cut(s) 1928, 1990, 2050
Eco47I GGWCC 2 cut(s) 1949, 2165
Eco57I CTGAAG 1 cut(s) 515
EcoRII CCWGG 5 cut(s) 232, 356, 1215, 1297, 1753
EcoRV GATATC 3 cut(s) 1928, 1990, 2050
EcoT14I CCWWGG 3 cut(s) 81, 697, 2334
EcoT22I ATGCAT 3 cut(s) 304, 430, 1473
EcoT38I GRGCYC 1 cut(s) 1004
ErhI CCWWGG 3 cut(s) 81, 697, 2334
FalI AAGNNNNNCTT 3 cut(s) 513, 545, 2558
FaqI GGGAC 3 cut(s) 374, 1258, 1962
FauNDI CATATG 3 cut(s) 548, 1954, 2265
FbaI TGATCA 2 cut(s) 669, 2254
FblI GTMKAC 1 cut(s) 845
Fnu4HI GCNGC 6 cut(s) 211, 663, 1265, 1837, 2445, 2448
FriOI GRGCYC 1 cut(s) 1004
Fsp4HI GCNGC 6 cut(s) 211, 663, 1265, 1837, 2445, 2448
FspBI CTAG 4 cut(s) 435, 1860, 2525, 2578
GlaI GCGC 2 cut(s) 613, 1896
GluI GCNGC 6 cut(s) 211, 663, 1265, 1837, 2445, 2448
GsuI CTGGAG 1 cut(s) 340
HaeII RGCGCY 1 cut(s) 615
HaeIII GGCC 3 cut(s) 921, 1390, 2413
HapII CCGG 3 cut(s) 1242, 1964, 2389
HgaI GACGC 1 cut(s) 1762
HhaI GCGC 2 cut(s) 614, 1897
Hin6I GCGC 2 cut(s) 612, 1895
HinP1I GCGC 2 cut(s) 612, 1895
HincII GTYRAC 1 cut(s) 1378
HindII GTYRAC 1 cut(s) 1378
HindIII AAGCTT 3 cut(s) 56, 1786, 2205
HinfI GANTC 8 cut(s) 395, 805, 1012, 1312, 1665, 1821, 2090, 2456
HpaII CCGG 3 cut(s) 1242, 1964, 2389
HphI GGTGA 2 cut(s) 575, 1729
Hpy166II GTNNAC 8 cut(s) 846, 1178, 1344, 1378, 1777, 1853, 1961, 2299
Hpy188I TCNGA 9 cut(s) 534, 580, 754, 773, 1317, 1329, 1932, 2383, 2437
Hpy188III TCNNGA 9 cut(s) 269, 473, 793, 1492, 1913, 2087, 2108, 2168, 2463
Hpy8I GTNNAC 8 cut(s) 846, 1178, 1344, 1378, 1777, 1853, 1961, 2299
Hpy99I CGWCG 1 cut(s) 151
HpyAV CCTTC 8 cut(s) 110, 239, 394, 435, 1744, 1916, 2038, 2300
HpyCH4III ACNGT 2 cut(s) 1324, 1386
HpyCH4IV ACGT 1 cut(s) 149
HpyF10VI GCNNNNNNNGC 3 cut(s) 2321, 2330, 2347
HpyF3I CTNAG 9 cut(s) 734, 1008, 1088, 1811, 2100, 2358, 2380, 2510, 2542
HpySE526I ACGT 1 cut(s) 149
HspAI GCGC 2 cut(s) 612, 1895
Ksp22I TGATCA 2 cut(s) 669, 2254
Kzo9I GATC 6 cut(s) 40, 465, 669, 1076, 1935, 2254
LmnI GCTCC 5 cut(s) 371, 1807, 2018, 2200, 2515
Lsp1109I GCAGC 5 cut(s) 222, 674, 1276, 1848, 2431
LweI GCATC 7 cut(s) 133, 289, 415, 1738, 2046, 2113, 2302
MaeI CTAG 4 cut(s) 435, 1860, 2525, 2578
MaeII ACGT 1 cut(s) 149
MaeIII GTNAC 5 cut(s) 101, 124, 551, 815, 1475
MalI GATC 6 cut(s) 42, 467, 671, 1078, 1937, 2256
MboI GATC 6 cut(s) 40, 465, 669, 1076, 1935, 2254
MfeI CAATTG 2 cut(s) 66, 1554
MhlI GDGCHC 3 cut(s) 1004, 1706, 1963
MlsI TGGCCA 1 cut(s) 1390
MluNI TGGCCA 1 cut(s) 1390
MlyI GAGTC 1 cut(s) 1006
MmeI TCCRAC 4 cut(s) 162, 702, 2460, 2516
Mox20I TGGCCA 1 cut(s) 1390
Mph1103I ATGCAT 3 cut(s) 304, 430, 1473
MscI TGGCCA 1 cut(s) 1390
MslI CAYNNNNRTG 1 cut(s) 383
Msp20I TGGCCA 1 cut(s) 1390
MspCI CTTAAG 2 cut(s) 97, 1697
MspI CCGG 3 cut(s) 1242, 1964, 2389
MspR9I CCNGG 6 cut(s) 234, 358, 1217, 1243, 1299, 1755
MunI CAATTG 2 cut(s) 66, 1554
Mva1269I GAATGC 1 cut(s) 1897
MvaI CCWGG 5 cut(s) 234, 358, 1217, 1299, 1755
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 3 cut(s) 2321, 2330, 2347
NciI CCSGG 1 cut(s) 1243
NcoI CCATGG 2 cut(s) 81, 2334
NdeI CATATG 3 cut(s) 548, 1954, 2265
NdeII GATC 6 cut(s) 40, 465, 669, 1076, 1935, 2254
NlaIV GGNNCC 4 cut(s) 231, 373, 1950, 2166
NmuCI GTSAC 2 cut(s) 124, 551
NsiI ATGCAT 3 cut(s) 304, 430, 1473
NspI RCATGY 5 cut(s) 19, 984, 1172, 2143, 2357
NspV TTCGAA 1 cut(s) 1824
PagI TCATGA 1 cut(s) 1912
PceI AGGCCT 2 cut(s) 921, 2413
PciI ACATGT 1 cut(s) 15
PctI GAATGC 1 cut(s) 1897
PfeI GAWTC 7 cut(s) 395, 805, 1312, 1665, 1821, 2090, 2456
PflMI CCANNNNNTGG 1 cut(s) 51
PfoI TCCNGGA 1 cut(s) 1297
PkrI GCNGC 6 cut(s) 212, 664, 1266, 1838, 2446, 2449
PleI GAGTC 1 cut(s) 1006
PpsI GAGTC 1 cut(s) 1006
PscI ACATGT 1 cut(s) 15
PshBI ATTAAT 1 cut(s) 1587
PsiI TTATAA 3 cut(s) 1260, 1571, 2082
Psp6I CCWGG 5 cut(s) 232, 356, 1215, 1297, 1753
PspGI CCWGG 5 cut(s) 232, 356, 1215, 1297, 1753
PspN4I GGNNCC 4 cut(s) 231, 373, 1950, 2166
PspPI GGNCC 2 cut(s) 1949, 2165
PstI CTGCAG 1 cut(s) 856
PstNI CAGNNNCTG 1 cut(s) 2347
RsaI GTAC 5 cut(s) 979, 1646, 1688, 1852, 2300
RsaNI GTAC 5 cut(s) 978, 1645, 1687, 1851, 2299
RseI CAYNNNNRTG 1 cut(s) 383
SatI GCNGC 6 cut(s) 211, 663, 1265, 1837, 2445, 2448
Sau3AI GATC 6 cut(s) 40, 465, 669, 1076, 1935, 2254
Sau96I GGNCC 2 cut(s) 1949, 2165
SchI GAGTC 1 cut(s) 1006
ScrFI CCNGG 6 cut(s) 234, 358, 1217, 1243, 1299, 1755
SduI GDGCHC 3 cut(s) 1004, 1706, 1963
SfaNI GCATC 7 cut(s) 133, 289, 415, 1738, 2046, 2113, 2302
SfcI CTRYAG 1 cut(s) 852
SfuI TTCGAA 1 cut(s) 1824
SinI GGWCC 2 cut(s) 1949, 2165
SmiMI CAYNNNNRTG 1 cut(s) 383
SmlI CTYRAG 4 cut(s) 97, 1016, 1697, 1768
SmoI CTYRAG 4 cut(s) 97, 1016, 1697, 1768
SseBI AGGCCT 2 cut(s) 921, 2413
SsiI CCGC 9 cut(s) 157, 227, 322, 338, 618, 940, 1068, 1613, 2448
SspMI CTAG 4 cut(s) 435, 1860, 2525, 2578
StuI AGGCCT 2 cut(s) 921, 2413
StyD4I CCNGG 6 cut(s) 232, 356, 1215, 1241, 1297, 1753
StyI CCWWGG 3 cut(s) 81, 697, 2334
TaaI ACNGT 2 cut(s) 1324, 1386
TaiI ACGT 1 cut(s) 152
TaqI TCGA 6 cut(s) 398, 543, 1075, 1157, 1824, 2093
TatI WGTACW 2 cut(s) 1644, 1850
TauI GCSGC 1 cut(s) 2450
TfiI GAWTC 7 cut(s) 395, 805, 1312, 1665, 1821, 2090, 2456
TscAI CASTG 4 cut(s) 175, 753, 1146, 1353
TseFI GTSAC 2 cut(s) 124, 551
TseI GCWGC 5 cut(s) 210, 662, 1264, 1836, 2444
Tsp45I GTSAC 2 cut(s) 124, 551
TspDTI ATGAA 5 cut(s) 26, 525, 1219, 1929, 2469
TspGWI ACGGA 2 cut(s) 1820, 2107
TspRI CASTG 4 cut(s) 175, 753, 1146, 1353
Van91I CCANNNNNTGG 1 cut(s) 51
Vha464I CTTAAG 2 cut(s) 97, 1697
VneI GTGCAC 1 cut(s) 1959
VpaK11BI GGWCC 2 cut(s) 1949, 2165
VspI ATTAAT 1 cut(s) 1587
XapI RAATTY 5 cut(s) 365, 404, 836, 1121, 2528
XceI RCATGY 5 cut(s) 19, 984, 1172, 2143, 2357
XmiI GTMKAC 1 cut(s) 845
XspI CTAG 4 cut(s) 435, 1860, 2525, 2578
Zsp2I ATGCAT 3 cut(s) 304, 430, 1473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.