pycom03g08540

respiratory burst oxidase homolog protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010309
Physical Location & Seq
Forward (+)
6902707 .. 6903564
858 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g08540.1

Sequence Viewer

Length: 858 bp
ATGCAGATGTGGCAGTTAGAAACCCTACTGAGGGGAATGGTAGTATCTAAAGATAATCAAAAAATTGGAATGATAACGGAATCTCTCACGAGAGCCATGATTCCCAGAAGATACAGAACTCCCATTAGCAGATTCCTATCTACGACTGCAGAACTTGTGCATGAAAACTGGAAGAAAATATGGATTGTGACATTGTGGTTAGCTGTCAACATAGGCCTCTTTACTTGGAAATACCGCCAATACCAGACACGAGGATTATACAAACTCATGGGTGAATGCATCTGCATTGCAAAGGGCTGCGCTGAGACTCTCAAGTTCAATATGGCTATCATTCTCATTCCAGTTTGTAGGGGAACACTTACCGCAATTCGATCATCATTTCTCAGCAACCTAATCCCTTTTGATGACAATATCAAATTCCATAAGTTGATTGCACTGGGAATTGTAATTTCGACTTTTATACATGGAGGTTTACACATAACTTGTGATTTCCCAAGATTGGTAACATCCCAGAAAAATGAGTTCATGGATGTTGTCGGGACATATTTCAATTATAAGCAGCCAACTTACATGGACATGATTAACAGTATTCCAGGAATAACCGGGATTCTGATGACGGTTCTAATGATTTTCTCATTCACGCTAGCATTAAATTCAATGAGAACAAATGTCAACTTACCGTGGCCACTCAACAATTTGTCAGGGTTCAATTCTTTCTGGTATGCACATCATTTGCTTGCGTTGGTGTATATGCTCTTCGTCGCACATGGTTACTTCCTAGTTTTCGCGAGCGACTGGCACAAGAAGACGGTATATACGCTACTTAAATTTAGCTTCATATTCTCTGAATCACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04624 map04013 map04624
Pfam Domains
Protein Families
EF-hand_6 FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

286

Amino Acids

32.97

Weight (kDa)

9.57

Isoelectric Point (pI)

27.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferric_reduct PF01794 109 - 254 4.4e-13 Ferric reductase like transmembrane component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 555
AccII CGCG 1 cut(s) 788
AciI CCGC 2 cut(s) 235, 363
AcoI YGGCCR 1 cut(s) 683
AcsI RAATTY 3 cut(s) 416, 652, 827
AfiI CCNNNNNNNGG 3 cut(s) 30, 31, 499
AgsI TTSAA 4 cut(s) 319, 550, 657, 709
AjnI CCWGG 1 cut(s) 592
AluBI AGCT 2 cut(s) 203, 834
AluI AGCT 2 cut(s) 203, 834
Alw26I GTCTC 1 cut(s) 299
AoxI GGCC 2 cut(s) 214, 683
ApeKI GCWGC 2 cut(s) 297, 559
ApoI RAATTY 3 cut(s) 416, 652, 827
AspLEI GCGC 1 cut(s) 302
AsuC2I CCSGG 1 cut(s) 604
AsuHPI GGTGA 1 cut(s) 284
AsuNHI GCTAGC 1 cut(s) 643
BalI TGGCCA 1 cut(s) 685
BarI GAAGNNNNNNTAC 2 cut(s) 740, 772
BauI CACGAG 2 cut(s) 88, 249
BbsI GAAGAC 1 cut(s) 812
BbvI GCAGC 2 cut(s) 284, 571
BciT130I CCWGG 1 cut(s) 594
BcnI CCSGG 1 cut(s) 604
BcoDI GTCTC 1 cut(s) 299
BfaI CTAG 2 cut(s) 644, 779
BfmI CTRYAG 2 cut(s) 147, 854
BisI GCNGC 2 cut(s) 298, 560
BlsI GCNGC 2 cut(s) 299, 561
Bme1390I CCNGG 2 cut(s) 594, 604
BmrFI CCNGG 2 cut(s) 594, 604
BmrI ACTGGG 1 cut(s) 446
BmsI GCATC 1 cut(s) 288
BmtI GCTAGC 1 cut(s) 647
BmuI ACTGGG 1 cut(s) 446
BpiI GAAGAC 1 cut(s) 812
BpuEI CTTGAG 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 604
BsaBI GATNNNNATC 1 cut(s) 136
BsaJI CCNNGG 1 cut(s) 680
Bsc4I CCNNNNNNNGG 3 cut(s) 30, 31, 499
Bse1I ACTGG 4 cut(s) 173, 341, 441, 800
Bse3DI GCAATG 1 cut(s) 285
Bse8I GATNNNNATC 1 cut(s) 136
BseBI CCWGG 1 cut(s) 594
BseDI CCNNGG 1 cut(s) 680
BseGI GGATG 2 cut(s) 506, 535
BseJI GATNNNNATC 1 cut(s) 136
BseLI CCNNNNNNNGG 3 cut(s) 30, 31, 499
BseMI GCAATG 1 cut(s) 285
BseMII CTCAG 3 cut(s) 20, 294, 397
BseNI ACTGG 4 cut(s) 173, 341, 441, 800
BseXI GCAGC 2 cut(s) 284, 571
Bsh1236I CGCG 1 cut(s) 788
BshFI GGCC 2 cut(s) 216, 685
BsiSI CCGG 1 cut(s) 603
BslFI GGGAC 1 cut(s) 553
BslI CCNNNNNNNGG 3 cut(s) 30, 31, 499
BsmAI GTCTC 1 cut(s) 299
BsmFI GGGAC 1 cut(s) 553
BsmI GAATGC 1 cut(s) 281
BsnI GGCC 2 cut(s) 216, 685
Bsp143I GATC 1 cut(s) 371
Bsp68I TCGCGA 1 cut(s) 788
BspACI CCGC 2 cut(s) 235, 363
BspANI GGCC 2 cut(s) 216, 685
BspCNI CTCAG 3 cut(s) 21, 295, 396
BspFNI CGCG 1 cut(s) 788
BspMAI CTGCAG 1 cut(s) 151
BspOI GCTAGC 1 cut(s) 647
BspQI GCTCTTC 1 cut(s) 761
BsrDI GCAATG 1 cut(s) 285
BsrI ACTGG 4 cut(s) 173, 341, 441, 800
BssECI CCNNGG 1 cut(s) 680
BssMI GATC 1 cut(s) 371
BssSI CACGAG 2 cut(s) 88, 249
Bst2BI CACGAG 2 cut(s) 88, 249
Bst2UI CCWGG 1 cut(s) 594
Bst4CI ACNGT 4 cut(s) 587, 619, 681, 811
Bst6I CTCTTC 1 cut(s) 761
BstC8I GCNNGC 3 cut(s) 645, 738, 790
BstDEI CTNAG 3 cut(s) 29, 303, 383
BstDSI CCRYGG 1 cut(s) 680
BstF5I GGATG 2 cut(s) 506, 535
BstFNI CGCG 1 cut(s) 788
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 1 cut(s) 374
BstMAI GTCTC 1 cut(s) 299
BstMBI GATC 1 cut(s) 371
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 1 cut(s) 594
BstSCI CCNGG 2 cut(s) 592, 602
BstSFI CTRYAG 2 cut(s) 147, 854
BstUI CGCG 1 cut(s) 788
BstV1I GCAGC 2 cut(s) 284, 571
BstV2I GAAGAC 1 cut(s) 812
BsuRI GGCC 2 cut(s) 216, 685
BtgI CCRYGG 1 cut(s) 680
BtsCI GGATG 2 cut(s) 506, 535
BtsIMutI CAGTG 1 cut(s) 434
BtuMI TCGCGA 1 cut(s) 788
Cac8I GCNNGC 3 cut(s) 645, 738, 790
CfoI GCGC 1 cut(s) 302
CviAII CATG 8 cut(s) 97, 161, 268, 464, 526, 571, 577, 767
CviJI RGCY 8 cut(s) 95, 203, 216, 297, 326, 562, 685, 834
CviKI_1 RGCY 8 cut(s) 95, 203, 216, 297, 326, 562, 685, 834
DdeI CTNAG 3 cut(s) 29, 303, 383
DpnI GATC 1 cut(s) 373
DpnII GATC 1 cut(s) 371
EaeI YGGCCR 1 cut(s) 683
Eam1104I CTCTTC 1 cut(s) 761
EarI CTCTTC 1 cut(s) 761
Eco147I AGGCCT 1 cut(s) 216
EcoRII CCWGG 1 cut(s) 592
EcoT22I ATGCAT 1 cut(s) 281
FaeI CATG 8 cut(s) 100, 164, 271, 467, 529, 574, 580, 770
FaqI GGGAC 1 cut(s) 553
FatI CATG 8 cut(s) 96, 160, 267, 463, 525, 570, 576, 766
Fnu4HI GCNGC 2 cut(s) 298, 560
FokI GGATG 2 cut(s) 493, 542
Fsp4HI GCNGC 2 cut(s) 298, 560
FspBI CTAG 2 cut(s) 644, 779
GlaI GCGC 1 cut(s) 301
GluI GCNGC 2 cut(s) 298, 560
HaeIII GGCC 2 cut(s) 216, 685
HapII CCGG 1 cut(s) 603
HhaI GCGC 1 cut(s) 302
Hin1II CATG 8 cut(s) 100, 164, 271, 467, 529, 574, 580, 770
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HincII GTYRAC 2 cut(s) 208, 673
HindII GTYRAC 2 cut(s) 208, 673
HinfI GANTC 6 cut(s) 80, 100, 132, 307, 607, 848
HpaII CCGG 1 cut(s) 603
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 3 cut(s) 208, 473, 673
Hpy188I TCNGA 2 cut(s) 612, 847
Hpy188III TCNNGA 3 cut(s) 88, 538, 787
Hpy8I GTNNAC 3 cut(s) 208, 473, 673
Hpy99I CGWCG 1 cut(s) 764
HpyCH4III ACNGT 4 cut(s) 587, 619, 681, 811
HpyCH4V TGCA 8 cut(s) 4, 149, 160, 279, 285, 290, 434, 725
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 3 cut(s) 29, 303, 383
Hsp92II CATG 8 cut(s) 100, 164, 271, 467, 529, 574, 580, 770
HspAI GCGC 1 cut(s) 300
Kzo9I GATC 1 cut(s) 371
LguI GCTCTTC 1 cut(s) 761
Lsp1109I GCAGC 2 cut(s) 284, 571
LweI GCATC 1 cut(s) 288
MaeI CTAG 2 cut(s) 644, 779
MaeIII GTNAC 3 cut(s) 187, 502, 770
MalI GATC 1 cut(s) 373
MboI GATC 1 cut(s) 371
MboII GAAGA 4 cut(s) 120, 184, 748, 817
MlsI TGGCCA 1 cut(s) 685
MluNI TGGCCA 1 cut(s) 685
MlyI GAGTC 1 cut(s) 301
MnlI CCTC 4 cut(s) 24, 227, 245, 461
Mox20I TGGCCA 1 cut(s) 685
Mph1103I ATGCAT 1 cut(s) 281
MscI TGGCCA 1 cut(s) 685
MseI TTAA 3 cut(s) 582, 650, 825
MslI CAYNNNNRTG 1 cut(s) 575
Msp20I TGGCCA 1 cut(s) 685
MspI CCGG 1 cut(s) 603
MspR9I CCNGG 2 cut(s) 594, 604
Mva1269I GAATGC 1 cut(s) 281
MvaI CCWGG 1 cut(s) 594
MvnI CGCG 1 cut(s) 788
MwoI GCNNNNNNNGC 1 cut(s) 10
NciI CCSGG 1 cut(s) 604
NdeII GATC 1 cut(s) 371
NheI GCTAGC 1 cut(s) 643
NlaIII CATG 8 cut(s) 100, 164, 271, 467, 529, 574, 580, 770
NmuCI GTSAC 1 cut(s) 187
NruI TCGCGA 1 cut(s) 788
NsiI ATGCAT 1 cut(s) 281
PceI AGGCCT 1 cut(s) 216
PciSI GCTCTTC 1 cut(s) 761
PctI GAATGC 1 cut(s) 281
PfeI GAWTC 5 cut(s) 80, 100, 132, 607, 848
PfoI TCCNGGA 1 cut(s) 592
PkrI GCNGC 2 cut(s) 299, 561
PleI GAGTC 1 cut(s) 301
PpsI GAGTC 1 cut(s) 301
PsiI TTATAA 1 cut(s) 555
Psp6I CCWGG 1 cut(s) 592
PspGI CCWGG 1 cut(s) 592
PstI CTGCAG 1 cut(s) 151
RruI TCGCGA 1 cut(s) 788
RseI CAYNNNNRTG 1 cut(s) 575
SapI GCTCTTC 1 cut(s) 761
SaqAI TTAA 3 cut(s) 582, 650, 825
SatI GCNGC 2 cut(s) 298, 560
Sau3AI GATC 1 cut(s) 371
SchI GAGTC 1 cut(s) 301
ScrFI CCNGG 2 cut(s) 594, 604
SetI ASST 4 cut(s) 205, 393, 472, 836
SfaNI GCATC 1 cut(s) 288
SfcI CTRYAG 2 cut(s) 147, 854
SmiMI CAYNNNNRTG 1 cut(s) 575
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
SseBI AGGCCT 1 cut(s) 216
SsiI CCGC 2 cut(s) 235, 363
SspMI CTAG 2 cut(s) 644, 779
StuI AGGCCT 1 cut(s) 216
StyD4I CCNGG 2 cut(s) 592, 602
TaaI ACNGT 4 cut(s) 587, 619, 681, 811
TaqI TCGA 2 cut(s) 370, 452
TfiI GAWTC 5 cut(s) 80, 100, 132, 607, 848
Tru1I TTAA 3 cut(s) 582, 650, 825
Tru9I TTAA 3 cut(s) 582, 650, 825
TscAI CASTG 1 cut(s) 441
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 2 cut(s) 297, 559
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 3 cut(s) 177, 514, 826
TspGWI ACGGA 1 cut(s) 92
TspRI CASTG 1 cut(s) 441
XapI RAATTY 3 cut(s) 416, 652, 827
XspI CTAG 2 cut(s) 644, 779
Zsp2I ATGCAT 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.