MD11G1147300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0006346
Physical Location & Seq
Forward (+)
13870354 .. 13875624
5271 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1147300.v1.1.491

Sequence Viewer

Length: 1383 bp
ATGGTGGACATGGAGGATAACTTCCTTGATTTCGAGTCAGAAGACCCACTTCTCACTCGCCCTGCCGTTACCAAGAGAAGGAGGAAGACTATTGGGTTGGATGACCTTTTGACTGACTTCTACAAGGAAGAAGACAAGATTGTTGAAAAGGAAGCTAAGCGTGCAAAAGCACGAAGGAACAATGATTCAGATGAAGAAGATAATGACAAAGAGGCTGCTCTCTTTGACATTGTCGATAAGTGTGAAAGCGAGATGAGAGAAATAAGTGGTAAGGAGGAGAGCTTTGTGTGGGGCATATGCGCTTTTGGAGATCAGAAGACCCCACCTCCTCTATCTTTTCCCGAGCTTAAAACTTGCAGAGTCTTGCGGTCATTCATGGATGATGAGCTCAATTCTTTGATTGATATCTCTACAGAAAATGGAGACACTTTCCTTGAACCATTATTAGTAAATGGCTGGCTATCAAAATTGGTTTTCGCCAATGGTCATCTAGAAAAATCAATAGCCACATGGACCTTTTATCTGATGTTATATTCGTCTAAAGAAAATTTGAGGGCATCTGCATGTGACTTCTGGTGTGCTATCCTCTCATTTGAAAATGAGGTTGACTTACCGCCAGTCAAAATTGATTGGTTTCCTAGCTATTTTGAATTAAAAATAGCTCTTGAAAACTATGGATTTCTATTCAACTTTTCATCTAACACGGATTCTGCTCCTTCCAATTCTGCTCGTAGAGGTCCAGCTCCAAATATTAGAGCTTGGCTTAAATTCGTGACTGCTGCTTGTCAAAAGAGGAACAAAATGGCTATGTACTTGACCTCAGAAGCCGAAGAGTTAATTGAAGTCATTATTTGTCTATTTTCTGATCGCCAACTTCAAGGTCTGTCCATGCTTTTACATGAGTGCATGCAGTCAGCTATCAGTTACTTCACTGAAAATGAGTGGGAATCTAGCTGTGAAAAGATAGCAAATTCTCTTGCTTGCAGAGTTCCTAAAGACGTGACCTGCCTAAGAATTGTGGAATGCATTTCGGGAGTCGATACTCGTAGTAAGCTGTTTAGAAGTGCAATTGCCCATCAGATGCTTCTTAGCTGTTATGATCATAAGGCCCCTAACGATGAAGAGATCCTAAAATTGCTTATCCCAGTAAATGTGAAGGACAAAAAATGTGATTTTTCCAAGATGTACATACACTTGGTATTAGCCGAGAATTGGCTTTTGTCCAGTCAACTAGTAGAAGACAAGCCCGTCTTGAAAGCAATGTGGCGCCTCTACTTACGAAACTGTTCTTGCCTGATAGCCAGTACAGATTTGAGGTCTTTTGCATCGAAGGTCCGTAACAAGGCTTCGTACCTTCTGCAAGGCACCATCACAGCTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAM178

Protein Analysis

461

Amino Acids

52.68

Weight (kDa)

5.07

Isoelectric Point (pI)

56.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1247
Acc36I ACCTGC 1 cut(s) 1013
AccB1I GGYRCC 2 cut(s) 1266, 1364
AciI CCGC 2 cut(s) 367, 614
AclWI GGATC 1 cut(s) 1120
AcsI RAATTY 3 cut(s) 547, 767, 970
AcyI GRCGYC 1 cut(s) 1267
AfaI GTAC 4 cut(s) 812, 1187, 1306, 1352
AfiI CCNNNNNNNGG 3 cut(s) 78, 1212, 1342
AgsI TTSAA 9 cut(s) 146, 437, 596, 650, 668, 688, 842, 878, 1255
AhlI ACTAGT 1 cut(s) 1231
AjiI CACGTC 1 cut(s) 1000
Alw21I GWGCWC 1 cut(s) 390
Alw26I GTCTC 1 cut(s) 417
AlwI GGATC 1 cut(s) 1120
Ama87I CYCGRG 1 cut(s) 341
AoxI GGCC 1 cut(s) 1107
ApeKI GCWGC 2 cut(s) 215, 779
ApoI RAATTY 3 cut(s) 547, 767, 970
Asp700I GAANNNNTTC 1 cut(s) 1285
AspLEI GCGC 2 cut(s) 302, 1269
AspS9I GGNCC 4 cut(s) 513, 737, 1108, 1333
AvaI CYCGRG 1 cut(s) 341
AvaII GGWCC 3 cut(s) 513, 737, 1333
BanI GGYRCC 2 cut(s) 1266, 1364
BanII GRGCYC 1 cut(s) 390
BarI GAAGNNNNNNTAC 2 cut(s) 1330, 1362
BbsI GAAGAC 5 cut(s) 48, 92, 138, 323, 1245
Bbv12I GWGCWC 1 cut(s) 390
BbvI GCAGC 2 cut(s) 202, 766
BccI CCATC 2 cut(s) 1083, 1376
BceAI ACGGC 1 cut(s) 50
BclI TGATCA 1 cut(s) 1099
BcoDI GTCTC 1 cut(s) 417
BcuI ACTAGT 1 cut(s) 1231
BfaI CTAG 4 cut(s) 491, 639, 951, 1232
BfmI CTRYAG 1 cut(s) 411
BfoI RGCGCY 1 cut(s) 1270
BfuAI ACCTGC 1 cut(s) 1013
BisI GCNGC 2 cut(s) 216, 780
BlpI GCTNAGC 1 cut(s) 156
BlsI GCNGC 2 cut(s) 217, 781
Bme18I GGWCC 3 cut(s) 513, 737, 1333
BmeT110I CYCGRG 1 cut(s) 341
BmgBI CACGTC 1 cut(s) 1000
BmgT120I GGNCC 4 cut(s) 513, 737, 1108, 1333
BmiI GGNNCC 3 cut(s) 1110, 1268, 1366
BmrI ACTGGG 1 cut(s) 1139
BmsI GCATC 3 cut(s) 566, 1071, 1334
BmuI ACTGGG 1 cut(s) 1139
BpiI GAAGAC 5 cut(s) 48, 92, 138, 323, 1245
Bpu1102I GCTNAGC 1 cut(s) 156
BsaBI GATNNNNATC 1 cut(s) 404
BsaHI GRCGYC 1 cut(s) 1267
BsaXI ACNNNNNCTCC 2 cut(s) 310, 340
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 1212, 1342
Bse1I ACTGG 4 cut(s) 617, 1145, 1224, 1302
Bse3DI GCAATG 1 cut(s) 1266
Bse8I GATNNNNATC 1 cut(s) 404
BseGI GGATG 2 cut(s) 106, 385
BseJI GATNNNNATC 1 cut(s) 404
BseLI CCNNNNNNNGG 3 cut(s) 78, 1212, 1342
BseMI GCAATG 1 cut(s) 1266
BseMII CTCAG 1 cut(s) 834
BseNI ACTGG 4 cut(s) 617, 1145, 1224, 1302
BseRI GAGGAG 2 cut(s) 290, 318
BseXI GCAGC 2 cut(s) 202, 766
BshFI GGCC 1 cut(s) 1109
BshNI GGYRCC 2 cut(s) 1266, 1364
BsiHKAI GWGCWC 1 cut(s) 390
BsiHKCI CYCGRG 1 cut(s) 341
BslI CCNNNNNNNGG 3 cut(s) 78, 1212, 1342
BsmAI GTCTC 1 cut(s) 417
BsmI GAATGC 1 cut(s) 1028
BsnI GGCC 1 cut(s) 1109
BsoBI CYCGRG 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 390
Bsp1407I TGTACA 1 cut(s) 1185
Bsp143I GATC 4 cut(s) 310, 865, 1099, 1125
Bsp1720I GCTNAGC 1 cut(s) 156
BspACI CCGC 2 cut(s) 367, 614
BspANI GGCC 1 cut(s) 1109
BspCNI CTCAG 1 cut(s) 833
BspLI GGNNCC 3 cut(s) 1110, 1268, 1366
BspMI ACCTGC 1 cut(s) 1013
BspPI GGATC 1 cut(s) 1120
BspT107I GGYRCC 2 cut(s) 1266, 1364
BsrDI GCAATG 1 cut(s) 1266
BsrGI TGTACA 1 cut(s) 1185
BsrI ACTGG 4 cut(s) 617, 1145, 1224, 1302
BssMI GATC 4 cut(s) 310, 865, 1099, 1125
BssNI GRCGYC 1 cut(s) 1267
Bst4CI ACNGT 1 cut(s) 1286
Bst6I CTCTTC 2 cut(s) 825, 1116
BstACI GRCGYC 1 cut(s) 1267
BstAUI TGTACA 1 cut(s) 1185
BstC8I GCNNGC 4 cut(s) 162, 458, 908, 982
BstDEI CTNAG 4 cut(s) 156, 820, 1010, 1088
BstF5I GGATG 2 cut(s) 106, 385
BstH2I RGCGCY 1 cut(s) 1270
BstHHI GCGC 2 cut(s) 302, 1269
BstKTI GATC 4 cut(s) 313, 868, 1102, 1128
BstMAI GTCTC 1 cut(s) 417
BstMBI GATC 4 cut(s) 310, 865, 1099, 1125
BstMWI GCNNNNNNNGC 1 cut(s) 161
BstNSI RCATGY 2 cut(s) 567, 910
BstSFI CTRYAG 1 cut(s) 411
BstV1I GCAGC 2 cut(s) 202, 766
BstV2I GAAGAC 5 cut(s) 48, 92, 138, 323, 1245
BstX2I RGATCY 1 cut(s) 1125
BstYI RGATCY 1 cut(s) 1125
BsuRI GGCC 1 cut(s) 1109
BtrI CACGTC 1 cut(s) 1000
BtsCI GGATG 2 cut(s) 106, 385
BtsIMutI CAGTG 1 cut(s) 930
BveI ACCTGC 1 cut(s) 1013
Cac8I GCNNGC 4 cut(s) 162, 458, 908, 982
CfoI GCGC 2 cut(s) 302, 1269
Cfr13I GGNCC 4 cut(s) 513, 737, 1108, 1333
Csp6I GTAC 4 cut(s) 811, 1186, 1305, 1351
CviAII CATG 7 cut(s) 10, 376, 510, 564, 889, 899, 907
CviQI GTAC 4 cut(s) 811, 1186, 1305, 1351
DdeI CTNAG 4 cut(s) 156, 820, 1010, 1088
DinI GGCGCC 1 cut(s) 1268
DpnI GATC 4 cut(s) 312, 867, 1101, 1127
DpnII GATC 4 cut(s) 310, 865, 1099, 1125
DrdI GACNNNNNNGTC 1 cut(s) 1247
DseDI GACNNNNNNGTC 1 cut(s) 1247
Eam1104I CTCTTC 2 cut(s) 825, 1116
EarI CTCTTC 2 cut(s) 825, 1116
Ecl136II GAGCTC 1 cut(s) 388
Eco24I GRGCYC 1 cut(s) 390
Eco32I GATATC 1 cut(s) 406
Eco47I GGWCC 3 cut(s) 513, 737, 1333
Eco53kI GAGCTC 1 cut(s) 388
Eco88I CYCGRG 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 388
EcoO109I RGGNCCY 1 cut(s) 1108
EcoRV GATATC 1 cut(s) 406
EcoT22I ATGCAT 1 cut(s) 1028
EcoT38I GRGCYC 1 cut(s) 390
EgeI GGCGCC 1 cut(s) 1268
EheI GGCGCC 1 cut(s) 1268
FaeI CATG 7 cut(s) 13, 379, 513, 567, 892, 902, 910
FalI AAGNNNNNCTT 4 cut(s) 33, 65, 1235, 1267
FatI CATG 7 cut(s) 9, 375, 509, 563, 888, 898, 906
FauNDI CATATG 1 cut(s) 296
FbaI TGATCA 1 cut(s) 1099
Fnu4HI GCNGC 2 cut(s) 216, 780
FokI GGATG 2 cut(s) 113, 392
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 2 cut(s) 216, 780
FspBI CTAG 4 cut(s) 491, 639, 951, 1232
GlaI GCGC 2 cut(s) 301, 1268
GluI GCNGC 2 cut(s) 216, 780
HaeII RGCGCY 1 cut(s) 1270
HaeIII GGCC 1 cut(s) 1109
HhaI GCGC 2 cut(s) 302, 1269
Hin1I GRCGYC 1 cut(s) 1267
Hin1II CATG 7 cut(s) 13, 379, 513, 567, 892, 902, 910
Hin6I GCGC 2 cut(s) 300, 1267
HinP1I GCGC 2 cut(s) 300, 1267
HincII GTYRAC 2 cut(s) 607, 1229
HindII GTYRAC 2 cut(s) 607, 1229
HinfI GANTC 6 cut(s) 35, 185, 360, 707, 947, 1035
Hpy166II GTNNAC 3 cut(s) 7, 607, 1229
Hpy188I TCNGA 7 cut(s) 40, 190, 315, 525, 823, 865, 1080
Hpy188III TCNNGA 6 cut(s) 341, 491, 665, 772, 1032, 1252
Hpy8I GTNNAC 3 cut(s) 7, 607, 1229
HpyAV CCTTC 6 cut(s) 72, 168, 726, 1150, 1324, 1364
HpyCH4III ACNGT 1 cut(s) 1286
HpyCH4IV ACGT 1 cut(s) 999
HpyF10VI GCNNNNNNNGC 1 cut(s) 161
HpyF3I CTNAG 4 cut(s) 156, 820, 1010, 1088
HpySE526I ACGT 1 cut(s) 999
Hsp92I GRCGYC 1 cut(s) 1267
Hsp92II CATG 7 cut(s) 13, 379, 513, 567, 892, 902, 910
HspAI GCGC 2 cut(s) 300, 1267
KasI GGCGCC 1 cut(s) 1266
Ksp22I TGATCA 1 cut(s) 1099
Kzo9I GATC 4 cut(s) 310, 865, 1099, 1125
LmnI GCTCC 2 cut(s) 718, 748
Lsp1109I GCAGC 2 cut(s) 202, 766
LweI GCATC 3 cut(s) 566, 1071, 1334
MaeI CTAG 4 cut(s) 491, 639, 951, 1232
MaeII ACGT 1 cut(s) 999
MaeIII GTNAC 6 cut(s) 67, 566, 772, 923, 1000, 1337
MalI GATC 4 cut(s) 312, 867, 1101, 1127
MboI GATC 4 cut(s) 310, 865, 1099, 1125
MfeI CAATTG 1 cut(s) 1068
MflI RGATCY 1 cut(s) 1125
MhlI GDGCHC 1 cut(s) 390
Mly113I GGCGCC 1 cut(s) 1267
MlyI GAGTC 3 cut(s) 44, 369, 1044
MmeI TCCRAC 1 cut(s) 78
Mph1103I ATGCAT 1 cut(s) 1028
MroXI GAANNNNTTC 1 cut(s) 1285
MseI TTAA 4 cut(s) 348, 653, 765, 836
MslI CAYNNNNRTG 1 cut(s) 562
MspA1I CMGCKG 1 cut(s) 1376
MunI CAATTG 1 cut(s) 1068
Mva1269I GAATGC 1 cut(s) 1028
MwoI GCNNNNNNNGC 1 cut(s) 161
NarI GGCGCC 1 cut(s) 1267
NdeI CATATG 1 cut(s) 296
NdeII GATC 4 cut(s) 310, 865, 1099, 1125
NlaIII CATG 7 cut(s) 13, 379, 513, 567, 892, 902, 910
NlaIV GGNNCC 3 cut(s) 1110, 1268, 1366
NmeAIII GCCGAG 1 cut(s) 1231
NmuCI GTSAC 3 cut(s) 566, 772, 1000
NsiI ATGCAT 1 cut(s) 1028
NspI RCATGY 2 cut(s) 567, 910
PaeI GCATGC 1 cut(s) 910
PctI GAATGC 1 cut(s) 1028
PdmI GAANNNNTTC 1 cut(s) 1285
PfeI GAWTC 3 cut(s) 185, 707, 947
PflFI GACNNNGTC 1 cut(s) 230
PkrI GCNGC 2 cut(s) 217, 781
PleI GAGTC 3 cut(s) 43, 368, 1043
PluTI GGCGCC 1 cut(s) 1270
PpsI GAGTC 3 cut(s) 43, 368, 1043
Psp124BI GAGCTC 1 cut(s) 390
PspN4I GGNNCC 3 cut(s) 1110, 1268, 1366
PspPI GGNCC 4 cut(s) 513, 737, 1108, 1333
PsuI RGATCY 1 cut(s) 1125
PsyI GACNNNGTC 1 cut(s) 230
PvuII CAGCTG 1 cut(s) 1376
RsaI GTAC 4 cut(s) 812, 1187, 1306, 1352
RsaNI GTAC 4 cut(s) 811, 1186, 1305, 1351
RseI CAYNNNNRTG 1 cut(s) 562
SacI GAGCTC 1 cut(s) 390
SaqAI TTAA 4 cut(s) 348, 653, 765, 836
SatI GCNGC 2 cut(s) 216, 780
Sau3AI GATC 4 cut(s) 310, 865, 1099, 1125
Sau96I GGNCC 4 cut(s) 513, 737, 1108, 1333
SchI GAGTC 3 cut(s) 44, 369, 1044
SduI GDGCHC 1 cut(s) 390
SfaNI GCATC 3 cut(s) 566, 1071, 1334
SfcI CTRYAG 1 cut(s) 411
SfoI GGCGCC 1 cut(s) 1268
SinI GGWCC 3 cut(s) 513, 737, 1333
SmiMI CAYNNNNRTG 1 cut(s) 562
SpeI ACTAGT 1 cut(s) 1231
SphI GCATGC 1 cut(s) 910
SsiI CCGC 2 cut(s) 367, 614
SspDI GGCGCC 1 cut(s) 1266
SspI AATATT 1 cut(s) 751
SspMI CTAG 4 cut(s) 491, 639, 951, 1232
SstI GAGCTC 1 cut(s) 390
TaaI ACNGT 1 cut(s) 1286
TaiI ACGT 1 cut(s) 1002
TaqI TCGA 4 cut(s) 33, 234, 1038, 1328
TatI WGTACW 3 cut(s) 810, 1185, 1304
TfiI GAWTC 3 cut(s) 185, 707, 947
Tru1I TTAA 4 cut(s) 348, 653, 765, 836
Tru9I TTAA 4 cut(s) 348, 653, 765, 836
TscAI CASTG 1 cut(s) 937
TseFI GTSAC 3 cut(s) 566, 772, 1000
TseI GCWGC 2 cut(s) 215, 779
Tsp45I GTSAC 3 cut(s) 566, 772, 1000
TspDTI ATGAA 4 cut(s) 207, 364, 684, 1134
TspGWI ACGGA 2 cut(s) 719, 1325
TspRI CASTG 1 cut(s) 937
Tth111I GACNNNGTC 1 cut(s) 230
VpaK11BI GGWCC 3 cut(s) 513, 737, 1333
XapI RAATTY 3 cut(s) 547, 767, 970
XbaI TCTAGA 1 cut(s) 490
XceI RCATGY 2 cut(s) 567, 910
XmnI GAANNNNTTC 1 cut(s) 1285
XspI CTAG 4 cut(s) 491, 639, 951, 1232
Zsp2I ATGCAT 1 cut(s) 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.