Rorug05G0304800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006346
Physical Location & Seq
Forward (+)
35473036 .. 35473953
918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0304800.1

Sequence Viewer

Length: 918 bp
ATGCAACGCTTCAAGATCCTAGAGTGGAACTGTAGGGGCCTCAATAATATTGAGACCCAAGATGCTTTAGTCAACATTTTCCGACAACAGAATCCCAGTATCCTTTTTTTATCAGAAACCCTAGCCATGCCAGATTTGCTCAATGATGTTCGCCGACGTATAGGGTTTGATGGTGTTCTCTGTTCACCATGGGAAGATGATTGTCGAGGCCTGGGACTCTTTCGGCGGAATGAGATCCTTGTGCGTCTGCGGAATTACTCGTCCAATCATATTGATGCAGAGGTAGGGGCGATAGGTTCAGAAGGTGTGTTTCGGTTCACCGGAATATATGGAGTGGCTACAACGACCAATCGAAACATCACTTGGAATTTGCTTCGAACCGTTGCAGCACAGGTCCAGCTCCCTTGGTTAATCGCCGGGGATTTCAATGAAATCCTGTGCTTGGCTGATAAGTCGGGTGGTCCGGCTCGTGGTGTTAACCAGATGAACAGATTTCGTCAAGCTCTGGTGGATTGTGGATTAATGGATATGGGATACGTGGGCTCTCGATTCACTTGGGCTAATTGCTTCACAAAAGAAAGACTTGATAGAGCATGTCAGAACTTCCAGTGGAGAGACTTATACCCGTTCTCCCGAGTTATCACTCTTCCTCTGAGTCGATCGGATCATTGTCCCTTACTCATTGAGATTAATCCGGAGAGGCCTATAGTGCGTCGCTCTCCTCGGCGTTTTCGTTTTGAAGAAATGTGGCTAAGTCATTCAGATTGTGGGCATGTAGTGAAGCAAGGTTGGATGTTGCCTTTTACTGGAGAGTCGATGGGTCAGGTAGACCGTAAGATCAAGCAAACAGGGAACCTTTTTCTCAGTTGGCATGAAGGGGTTTTCCAGCAACGACAGATTGAGTTGAGGTTAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAM178

Protein Analysis

305

Amino Acids

35.33

Weight (kDa)

8.94

Isoelectric Point (pI)

55.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 8 - 207 3.5e-08 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 828
AccIII TCCGGA 1 cut(s) 694
AciI CCGC 2 cut(s) 226, 250
AclWI GGATC 3 cut(s) 10, 229, 672
AcsI RAATTY 1 cut(s) 367
AfiI CCNNNNNNNGG 3 cut(s) 442, 470, 806
AgsI TTSAA 3 cut(s) 13, 427, 740
AjnI CCWGG 1 cut(s) 210
AjuI GAANNNNNNNTTGG 2 cut(s) 346, 378
AluBI AGCT 2 cut(s) 400, 503
AluI AGCT 2 cut(s) 400, 503
Alw26I GTCTC 2 cut(s) 47, 609
AlwI GGATC 3 cut(s) 10, 229, 672
Ama87I CYCGRG 1 cut(s) 633
Aor13HI TCCGGA 1 cut(s) 694
AoxI GGCC 3 cut(s) 37, 208, 701
ApeKI GCWGC 1 cut(s) 386
ApoI RAATTY 1 cut(s) 367
AseI ATTAAT 2 cut(s) 521, 690
AspS9I GGNCC 3 cut(s) 37, 394, 461
AsuC2I CCSGG 1 cut(s) 418
AsuHPI GGTGA 2 cut(s) 177, 310
AsuII TTCGAA 1 cut(s) 376
AvaI CYCGRG 1 cut(s) 633
AvaII GGWCC 2 cut(s) 394, 461
BanII GRGCYC 1 cut(s) 545
BauI CACGAG 1 cut(s) 468
BbvI GCAGC 1 cut(s) 398
BccI CCATC 2 cut(s) 164, 811
BciT130I CCWGG 1 cut(s) 212
BciVI GTATCC 2 cut(s) 110, 527
BcnI CCSGG 1 cut(s) 418
BcoDI GTCTC 2 cut(s) 47, 609
BfaI CTAG 2 cut(s) 20, 122
BfmI CTRYAG 2 cut(s) 31, 705
BfuI GTATCC 2 cut(s) 110, 527
BisI GCNGC 1 cut(s) 387
BlsI GCNGC 1 cut(s) 388
Bme1390I CCNGG 2 cut(s) 212, 418
Bme18I GGWCC 2 cut(s) 394, 461
BmeT110I CYCGRG 1 cut(s) 633
BmgT120I GGNCC 3 cut(s) 37, 394, 461
BmiI GGNNCC 2 cut(s) 38, 854
BmrFI CCNGG 2 cut(s) 212, 418
BmrI ACTGGG 1 cut(s) 90
BmsI GCATC 2 cut(s) 52, 265
BmuI ACTGGG 1 cut(s) 90
BpmI CTGGAG 1 cut(s) 828
Bpu14I TTCGAA 1 cut(s) 376
BpuMI CCSGG 1 cut(s) 418
BsaAI YACGTR 1 cut(s) 538
BsaI GGTCTC 1 cut(s) 47
BsaJI CCNNGG 5 cut(s) 188, 211, 404, 417, 722
BsaWI WCCGGW 2 cut(s) 320, 694
BsaXI ACNNNNNCTCC 2 cut(s) 614, 644
Bsc4I CCNNNNNNNGG 3 cut(s) 442, 470, 806
Bse1I ACTGG 3 cut(s) 96, 607, 811
BseAI TCCGGA 1 cut(s) 694
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 5 cut(s) 188, 211, 404, 417, 722
BseGI GGATG 1 cut(s) 798
BseLI CCNNNNNNNGG 3 cut(s) 442, 470, 806
BseMII CTCAG 2 cut(s) 644, 877
BseNI ACTGG 3 cut(s) 96, 607, 811
BseRI GAGGAG 1 cut(s) 711
BseXI GCAGC 1 cut(s) 398
Bsh1285I CGRYCG 1 cut(s) 662
BshFI GGCC 3 cut(s) 39, 210, 703
BsiEI CGRYCG 1 cut(s) 662
BsiHKCI CYCGRG 1 cut(s) 633
BsiSI CCGG 4 cut(s) 321, 417, 464, 695
BslFI GGGAC 2 cut(s) 228, 657
BslI CCNNNNNNNGG 3 cut(s) 442, 470, 806
BsmAI GTCTC 2 cut(s) 47, 609
BsmFI GGGAC 2 cut(s) 228, 657
BsnI GGCC 3 cut(s) 39, 210, 703
Bso31I GGTCTC 1 cut(s) 47
BsoBI CYCGRG 1 cut(s) 633
Bsp119I TTCGAA 1 cut(s) 376
Bsp1286I GDGCHC 1 cut(s) 545
Bsp13I TCCGGA 1 cut(s) 694
Bsp143I GATC 5 cut(s) 15, 234, 659, 664, 837
Bsp19I CCATGG 1 cut(s) 188
BspACI CCGC 2 cut(s) 226, 250
BspANI GGCC 3 cut(s) 39, 210, 703
BspCNI CTCAG 2 cut(s) 645, 876
BspEI TCCGGA 1 cut(s) 694
BspLI GGNNCC 2 cut(s) 38, 854
BspPI GGATC 3 cut(s) 10, 229, 672
BspT104I TTCGAA 1 cut(s) 376
BspTNI GGTCTC 1 cut(s) 47
BsrI ACTGG 3 cut(s) 96, 607, 811
BssECI CCNNGG 5 cut(s) 188, 211, 404, 417, 722
BssMI GATC 5 cut(s) 15, 234, 659, 664, 837
BssSI CACGAG 1 cut(s) 468
BssT1I CCWWGG 2 cut(s) 188, 404
Bst2BI CACGAG 1 cut(s) 468
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 3 cut(s) 32, 382, 833
Bst6I CTCTTC 1 cut(s) 651
BstBAI YACGTR 1 cut(s) 538
BstBI TTCGAA 1 cut(s) 376
BstDEI CTNAG 3 cut(s) 653, 752, 863
BstDSI CCRYGG 1 cut(s) 188
BstF5I GGATG 1 cut(s) 798
BstKTI GATC 5 cut(s) 18, 237, 662, 667, 840
BstMAI GTCTC 2 cut(s) 47, 609
BstMBI GATC 5 cut(s) 15, 234, 659, 664, 837
BstMCI CGRYCG 1 cut(s) 662
BstMWI GCNNNNNNNGC 2 cut(s) 136, 709
BstNI CCWGG 1 cut(s) 212
BstNSI RCATGY 2 cut(s) 597, 776
BstSCI CCNGG 2 cut(s) 210, 416
BstSFI CTRYAG 2 cut(s) 31, 705
BstV1I GCAGC 1 cut(s) 398
BstX2I RGATCY 2 cut(s) 15, 234
BstYI RGATCY 2 cut(s) 15, 234
BsuI GTATCC 2 cut(s) 110, 527
BsuRI GGCC 3 cut(s) 39, 210, 703
BtgI CCRYGG 1 cut(s) 188
BtsCI GGATG 1 cut(s) 798
BtsIMutI CAGTG 1 cut(s) 614
Cfr13I GGNCC 3 cut(s) 37, 394, 461
CseI GACGC 2 cut(s) 233, 701
CviAII CATG 5 cut(s) 127, 189, 594, 773, 872
DdeI CTNAG 3 cut(s) 653, 752, 863
DpnI GATC 5 cut(s) 17, 236, 661, 666, 839
DpnII GATC 5 cut(s) 15, 234, 659, 664, 837
Eam1104I CTCTTC 1 cut(s) 651
EarI CTCTTC 1 cut(s) 651
EciI GGCGGA 1 cut(s) 241
Eco130I CCWWGG 2 cut(s) 188, 404
Eco147I AGGCCT 2 cut(s) 210, 703
Eco24I GRGCYC 1 cut(s) 545
Eco31I GGTCTC 1 cut(s) 47
Eco47I GGWCC 2 cut(s) 394, 461
Eco88I CYCGRG 1 cut(s) 633
EcoO109I RGGNCCY 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 210
EcoT14I CCWWGG 2 cut(s) 188, 404
EcoT38I GRGCYC 1 cut(s) 545
ErhI CCWWGG 2 cut(s) 188, 404
FaeI CATG 5 cut(s) 130, 192, 597, 776, 875
FalI AAGNNNNNCTT 2 cut(s) 567, 599
FaqI GGGAC 2 cut(s) 228, 657
FatI CATG 5 cut(s) 126, 188, 593, 772, 871
FblI GTMKAC 1 cut(s) 828
Fnu4HI GCNGC 1 cut(s) 387
FokI GGATG 1 cut(s) 805
FriOI GRGCYC 1 cut(s) 545
Fsp4HI GCNGC 1 cut(s) 387
FspBI CTAG 2 cut(s) 20, 122
GluI GCNGC 1 cut(s) 387
GsuI CTGGAG 1 cut(s) 828
HaeIII GGCC 3 cut(s) 39, 210, 703
HapII CCGG 4 cut(s) 321, 417, 464, 695
HgaI GACGC 2 cut(s) 233, 701
Hin1II CATG 5 cut(s) 130, 192, 597, 776, 875
HincII GTYRAC 2 cut(s) 73, 478
HindII GTYRAC 2 cut(s) 73, 478
HinfI GANTC 5 cut(s) 91, 216, 549, 655, 812
HpaI GTTAAC 1 cut(s) 478
HpaII CCGG 4 cut(s) 321, 417, 464, 695
HphI GGTGA 2 cut(s) 177, 310
Hpy166II GTNNAC 5 cut(s) 73, 185, 318, 478, 829
Hpy188I TCNGA 7 cut(s) 83, 115, 301, 600, 654, 664, 763
Hpy188III TCNNGA 4 cut(s) 13, 546, 633, 695
Hpy8I GTNNAC 5 cut(s) 73, 185, 318, 478, 829
Hpy99I CGWCG 2 cut(s) 159, 717
HpyAV CCTTC 2 cut(s) 296, 869
HpyCH4III ACNGT 3 cut(s) 32, 382, 833
HpyCH4IV ACGT 2 cut(s) 157, 537
HpyCH4V TGCA 3 cut(s) 4, 278, 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 136, 709
HpyF3I CTNAG 3 cut(s) 653, 752, 863
HpySE526I ACGT 2 cut(s) 157, 537
Hsp92II CATG 5 cut(s) 130, 192, 597, 776, 875
Kpn2I TCCGGA 1 cut(s) 694
KspAI GTTAAC 1 cut(s) 478
Kzo9I GATC 5 cut(s) 15, 234, 659, 664, 837
LmnI GCTCC 1 cut(s) 405
Lsp1109I GCAGC 1 cut(s) 398
LweI GCATC 2 cut(s) 52, 265
MaeI CTAG 2 cut(s) 20, 122
MaeII ACGT 2 cut(s) 157, 537
MalI GATC 5 cut(s) 17, 236, 661, 666, 839
MboI GATC 5 cut(s) 15, 234, 659, 664, 837
MboII GAAGA 3 cut(s) 206, 638, 752
MflI RGATCY 2 cut(s) 15, 234
MhlI GDGCHC 1 cut(s) 545
MluCI AATT 3 cut(s) 253, 367, 562
MlyI GAGTC 3 cut(s) 210, 664, 821
MmeI TCCRAC 2 cut(s) 106, 770
MnlI CCTC 7 cut(s) 50, 200, 274, 660, 693, 732, 900
MroI TCCGGA 1 cut(s) 694
MseI TTAA 5 cut(s) 410, 477, 521, 690, 911
MslI CAYNNNNRTG 1 cut(s) 273
MspI CCGG 4 cut(s) 321, 417, 464, 695
MspR9I CCNGG 2 cut(s) 212, 418
MvaI CCWGG 1 cut(s) 212
MwoI GCNNNNNNNGC 2 cut(s) 136, 709
NciI CCSGG 1 cut(s) 418
NcoI CCATGG 1 cut(s) 188
NdeII GATC 5 cut(s) 15, 234, 659, 664, 837
NlaIII CATG 5 cut(s) 130, 192, 597, 776, 875
NlaIV GGNNCC 2 cut(s) 38, 854
NmeAIII GCCGAG 1 cut(s) 703
NspI RCATGY 2 cut(s) 597, 776
NspV TTCGAA 1 cut(s) 376
PceI AGGCCT 2 cut(s) 210, 703
PcsI WCGNNNNNNNCGW 1 cut(s) 730
PfeI GAWTC 2 cut(s) 91, 549
PkrI GCNGC 1 cut(s) 388
Ple19I CGATCG 1 cut(s) 662
PleI GAGTC 3 cut(s) 210, 663, 820
PpsI GAGTC 3 cut(s) 210, 663, 820
Ppu21I YACGTR 1 cut(s) 538
PshBI ATTAAT 2 cut(s) 521, 690
Psp6I CCWGG 1 cut(s) 210
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 2 cut(s) 38, 854
PspPI GGNCC 3 cut(s) 37, 394, 461
PsuI RGATCY 2 cut(s) 15, 234
PvuI CGATCG 1 cut(s) 662
RseI CAYNNNNRTG 1 cut(s) 273
SaqAI TTAA 5 cut(s) 410, 477, 521, 690, 911
SatI GCNGC 1 cut(s) 387
Sau3AI GATC 5 cut(s) 15, 234, 659, 664, 837
Sau96I GGNCC 3 cut(s) 37, 394, 461
SchI GAGTC 3 cut(s) 210, 664, 821
ScrFI CCNGG 2 cut(s) 212, 418
SduI GDGCHC 1 cut(s) 545
SfaNI GCATC 2 cut(s) 52, 265
SfcI CTRYAG 2 cut(s) 31, 705
SfuI TTCGAA 1 cut(s) 376
SinI GGWCC 2 cut(s) 394, 461
SmiMI CAYNNNNRTG 1 cut(s) 273
Sse9I AATT 3 cut(s) 253, 367, 562
SseBI AGGCCT 2 cut(s) 210, 703
SsiI CCGC 2 cut(s) 226, 250
SspI AATATT 1 cut(s) 49
SspMI CTAG 2 cut(s) 20, 122
StuI AGGCCT 2 cut(s) 210, 703
StyD4I CCNGG 2 cut(s) 210, 416
StyI CCWWGG 2 cut(s) 188, 404
TaaI ACNGT 3 cut(s) 32, 382, 833
TaiI ACGT 2 cut(s) 160, 540
TaqI TCGA 6 cut(s) 205, 352, 376, 547, 658, 815
TasI AATT 3 cut(s) 253, 367, 562
TfiI GAWTC 2 cut(s) 91, 549
Tru1I TTAA 5 cut(s) 410, 477, 521, 690, 911
Tru9I TTAA 5 cut(s) 410, 477, 521, 690, 911
TscAI CASTG 1 cut(s) 614
TseI GCWGC 1 cut(s) 386
TspDTI ATGAA 3 cut(s) 444, 500, 888
TspRI CASTG 1 cut(s) 614
VpaK11BI GGWCC 2 cut(s) 394, 461
VspI ATTAAT 2 cut(s) 521, 690
XapI RAATTY 1 cut(s) 367
XceI RCATGY 2 cut(s) 597, 776
XmiI GTMKAC 1 cut(s) 828
XspI CTAG 2 cut(s) 20, 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.