Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0004144

Physical Location & Seq

Genomic Coordinates Forward (+)

35540841 .. 35544061

Gene Structure 3221 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1244700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 888 bp

ATGTCGACGTGCTCGTCGTCGTCCTCCTCTGGATCTTGGAACAGCACCAAGGCATGGATCGTCCACGGAATCGTCGCCGGAGTTGCGGTTGCGGTGGCAATTGGGGCCCGGGTCTACTTGGGTCCGACTAAGAAGTTCCGGAGCCGGGTCGTCGGCATCATACCTGCCCGCTTTGCTTCTTCTCGTTTCCAGGGCAAGCCCCTCGTTCAGATCCTCGGAAAACCCATGATCCAGAGAACATGGGAAAGAGCAAAACTTGCAAGTACACTGGATCGTGTTGTTGTGGCGACAGATGACGAGAGGATTCAAGAATGTTGTCACAGTTTTGGAGCTGATGTCGTAATGACCTCAGAATCCTGCCGGAACGGTACCGAGCGTTGCTCTGAAGCAATTGAGAAGCTCGAGAAGAATTATGATATAGTTGTCAATATTCAAGGGGATGAGCCTCTTATTGAACCTGAGATAATAGACGGAATTGTTAAAGCTTTGCAGGAAGCTCCAGATGCAGTGTTTAGTACTGCAGTCACGTCATTAAAATTTGAAGATGCATTGGATCCAAATCGAGTGAAATGTGTGGTGGATAATAGTGGTTATGCCATCTATTTTTCAAGGGGGCTGATTCCATTTAACAAGTCAGGAAAGGCGAATCCACAGTTTCCCTATTTGCTTCATCTAGGAATTCAGAGCTACGATACAAAGTTTCTAAAGATATATCCAGAACTTCAACCTACTCCTTTGCAACTAGAAGAGGATCTGGAGCAGCTTAAGGTCCTTGAAAATGGGTATAAGATGAAGGTGATTAAAGTTGACCATGAGGCTCACGGTGTTGACGCCCCTGAAGATGTTGAGAAGATTGAATCTCTAATGCGCGAAAGGAACTTGTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0006139 GO:0006725 GO:0006793 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008690 GO:0009058 GO:0009225 GO:0009226 GO:0009555 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0016020 GO:0016021 GO:0016043 GO:0016049 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0019867 GO:0022414 GO:0030154 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031306 GO:0031307 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032501 GO:0032502 GO:0032592 GO:0032989 GO:0033467 GO:0033468 GO:0034641 GO:0034654 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044706 GO:0046483 GO:0048229 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051704 GO:0055086 GO:0060560 GO:0070567 GO:0071704 GO:0071840 GO:0098573 GO:0098588 GO:0098805 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00540 ko01100 map00540 map01100

Pfam Domains

Protein Families

CTP_transf_3

Protein Analysis

296

Amino Acids

32.68

Weight (kDa)

5.84

Isoelectric Point (pI)

40.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CTP_transf_3	PF02348	51 - 263	1.3e-61	Cytidylyltransferase
NTP_transf_3	PF12804	60 - 171	1.6e-10	MobA-like NTP transferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004144)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53000 AT1G53000
fragaria_vesca	FvH4_3g20180 FvH4_3g20180 FvH4_3g20180
malus_domestica	MD03G1226400.v1.1 MD11G1244700.v1.1
prunus_persica	Prupe.4G047000_v2.0.a1 Prupe.4G047000_v2.0.a1 Prupe.4G183000_v2.0.a1 Prupe.4G183000_v2.0.a1
pyrus_communis	pycom03g17410 pycom11g21680
rosa_chinensis	RchiOBHm_Chr5g0008351 RchiOBHm_Chr5g0033991
rosa_laevigata	RLG00000033517
rosa_multiflora	Rmu_sc0012341.1_g000007 Rmu_sc0014753.1_g000003 Rmu_sc0021840.1_g000001
rosa_roxburghii	Rroxscaffold_1G00046240 Rroxscaffold_1G00068140
rosa_rugosa	Rorug04G0435400 Rorug04G0435400 Rorug05G0144000
rosa_samantha	Rh5AG063900 Rh5AG234900 Rh5BG060800 Rh5BG235700 Rh5CG070800 Rh5CG265500 Rh5DG059800
rosa_wichuraiana	Rw5G005710 Rw5G021470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	13
Acc36I	ACCTGC	1 cut(s)	172
Acc65I	GGTACC	1 cut(s)	368
AccB1I	GGYRCC	1 cut(s)	368
AccB7I	CCANNNNNTGG	1 cut(s)	54
AccI	GTMKAC	2 cut(s)	5, 114
AccII	CGCG	1 cut(s)	870
AccIII	TCCGGA	1 cut(s)	138
AciI	CCGC	3 cut(s)	86, 92, 169
AclWI	GGATC	8 cut(s)	40, 65, 205, 223, 279, 548, 561, 759
AcsI	RAATTY	2 cut(s)	536, 678
AcuI	CTGAAG	2 cut(s)	405, 858
AcyI	GRCGYC	1 cut(s)	831
AfaI	GTAC	3 cut(s)	265, 370, 517
AfiI	CCNNNNNNNGG	2 cut(s)	54, 145
AflII	CTTAAG	1 cut(s)	764
AgsI	TTSAA	8 cut(s)	308, 434, 455, 542, 609, 725, 776, 857
AjiI	CACGTC	2 cut(s)	9, 528
AjnI	CCWGG	1 cut(s)	189
AluBI	AGCT	6 cut(s)	332, 400, 485, 497, 687, 763
AluI	AGCT	6 cut(s)	332, 400, 485, 497, 687, 763
Alw21I	GWGCWC	1 cut(s)	14
AlwI	GGATC	8 cut(s)	40, 65, 205, 223, 279, 548, 561, 759
Ama87I	CYCGRG	2 cut(s)	108, 401
Aor13HI	TCCGGA	1 cut(s)	138
AoxI	GGCC	1 cut(s)	105
ApaI	GGGCCC	1 cut(s)	109
ApeKI	GCWGC	1 cut(s)	760
ApoI	RAATTY	2 cut(s)	536, 678
Asp718I	GGTACC	1 cut(s)	368
AspLEI	GCGC	1 cut(s)	870
AspS9I	GGNCC	4 cut(s)	105, 106, 122, 769
AsuC2I	CCSGG	3 cut(s)	109, 110, 146
AsuHPI	GGTGA	1 cut(s)	808
AvaI	CYCGRG	2 cut(s)	108, 401
AvaII	GGWCC	2 cut(s)	122, 769
BaeGI	GKGCMC	1 cut(s)	109
BamHI	GGATCC	1 cut(s)	553
BanI	GGYRCC	1 cut(s)	368
BanII	GRGCYC	1 cut(s)	109
Bbv12I	GWGCWC	1 cut(s)	14
BbvI	GCAGC	1 cut(s)	772
BccI	CCATC	1 cut(s)	605
BcgI	CGANNNNNNTGC	2 cut(s)	184, 218
BciT130I	CCWGG	1 cut(s)	191
BcnI	CCSGG	3 cut(s)	109, 110, 146
BfaI	CTAG	2 cut(s)	674, 743
BfmI	CTRYAG	1 cut(s)	519
BfrI	CTTAAG	1 cut(s)	764
BfuAI	ACCTGC	1 cut(s)	172
BisI	GCNGC	1 cut(s)	761
BlsI	GCNGC	1 cut(s)	762
BmcAI	AGTACT	1 cut(s)	517
Bme1390I	CCNGG	4 cut(s)	109, 110, 146, 191
Bme18I	GGWCC	2 cut(s)	122, 769
BmeT110I	CYCGRG	2 cut(s)	108, 401
BmgBI	CACGTC	2 cut(s)	9, 528
BmgT120I	GGNCC	4 cut(s)	105, 106, 122, 769
BmiI	GGNNCC	6 cut(s)	106, 107, 123, 143, 370, 555
BmrFI	CCNGG	4 cut(s)	109, 110, 146, 191
BmsI	GCATC	3 cut(s)	165, 493, 535
BplI	GAGNNNNNCTC	2 cut(s)	365, 397
BpmI	CTGGAG	2 cut(s)	483, 776
BpuMI	CCSGG	3 cut(s)	109, 110, 146
BsaBI	GATNNNNATC	1 cut(s)	558
BsaHI	GRCGYC	1 cut(s)	831
BsaJI	CCNNGG	5 cut(s)	48, 64, 108, 190, 214
BsaWI	WCCGGW	1 cut(s)	138
BsaXI	ACNNNNNCTCC	4 cut(s)	72, 102, 321, 351
Bsc4I	CCNNNNNNNGG	2 cut(s)	54, 145
Bse1I	ACTGG	1 cut(s)	273
Bse8I	GATNNNNATC	1 cut(s)	558
BseAI	TCCGGA	1 cut(s)	138
BseBI	CCWGG	1 cut(s)	191
BseDI	CCNNGG	5 cut(s)	48, 64, 108, 190, 214
BseGI	GGATG	1 cut(s)	445
BseJI	GATNNNNATC	1 cut(s)	558
BseLI	CCNNNNNNNGG	2 cut(s)	54, 145
BseMII	CTCAG	2 cut(s)	363, 450
BseNI	ACTGG	1 cut(s)	273
BseRI	GAGGAG	1 cut(s)	16
BseSI	GKGCMC	1 cut(s)	109
BseXI	GCAGC	1 cut(s)	772
Bsh1236I	CGCG	1 cut(s)	870
BshFI	GGCC	1 cut(s)	107
BshNI	GGYRCC	1 cut(s)	368
BsiHKAI	GWGCWC	1 cut(s)	14
BsiHKCI	CYCGRG	2 cut(s)	108, 401
BsiSI	CCGG	5 cut(s)	78, 109, 139, 145, 361
BslI	CCNNNNNNNGG	2 cut(s)	54, 145
BsnI	GGCC	1 cut(s)	107
BsoBI	CYCGRG	2 cut(s)	108, 401
Bsp120I	GGGCCC	1 cut(s)	105
Bsp1286I	GDGCHC	2 cut(s)	14, 109
Bsp13I	TCCGGA	1 cut(s)	138
Bsp143I	GATC	7 cut(s)	32, 57, 210, 228, 271, 553, 751
BspACI	CCGC	3 cut(s)	86, 92, 169
BspANI	GGCC	1 cut(s)	107
BspCNI	CTCAG	2 cut(s)	362, 451
BspEI	TCCGGA	1 cut(s)	138
BspFNI	CGCG	1 cut(s)	870
BspLI	GGNNCC	6 cut(s)	106, 107, 123, 143, 370, 555
BspMAI	CTGCAG	1 cut(s)	523
BspMI	ACCTGC	1 cut(s)	172
BspPI	GGATC	8 cut(s)	40, 65, 205, 223, 279, 548, 561, 759
BspT107I	GGYRCC	1 cut(s)	368
BspTI	CTTAAG	1 cut(s)	764
BsrI	ACTGG	1 cut(s)	273
BssECI	CCNNGG	5 cut(s)	48, 64, 108, 190, 214
BssMI	GATC	7 cut(s)	32, 57, 210, 228, 271, 553, 751
BssNI	GRCGYC	1 cut(s)	831
BssT1I	CCWWGG	1 cut(s)	48
Bst2UI	CCWGG	1 cut(s)	191
Bst4CI	ACNGT	4 cut(s)	323, 368, 654, 824
Bst6I	CTCTTC	1 cut(s)	741
BstACI	GRCGYC	1 cut(s)	831
BstAFI	CTTAAG	1 cut(s)	764
BstAPI	GCANNNNNTGC	1 cut(s)	257
BstC8I	GCNNGC	2 cut(s)	169, 197
BstDEI	CTNAG	4 cut(s)	129, 349, 459, 885
BstDSI	CCRYGG	1 cut(s)	64
BstF5I	GGATG	1 cut(s)	445
BstFNI	CGCG	1 cut(s)	870
BstHHI	GCGC	1 cut(s)	870
BstKTI	GATC	7 cut(s)	35, 60, 213, 231, 274, 556, 754
BstMBI	GATC	7 cut(s)	32, 57, 210, 228, 271, 553, 751
BstMWI	GCNNNNNNNGC	5 cut(s)	83, 104, 173, 257, 503
BstNI	CCWGG	1 cut(s)	191
BstSCI	CCNGG	4 cut(s)	107, 108, 144, 189
BstSFI	CTRYAG	1 cut(s)	519
BstSLI	GKGCMC	1 cut(s)	109
BstUI	CGCG	1 cut(s)	870
BstV1I	GCAGC	1 cut(s)	772
BstX2I	RGATCY	4 cut(s)	32, 210, 553, 751
BstYI	RGATCY	4 cut(s)	32, 210, 553, 751
BsuRI	GGCC	1 cut(s)	107
BtgI	CCRYGG	1 cut(s)	64
BtrI	CACGTC	2 cut(s)	9, 528
BtsCI	GGATG	1 cut(s)	445
BtsI	GCAGTG	1 cut(s)	513
BtsIMutI	CAGTG	2 cut(s)	266, 513
BveI	ACCTGC	1 cut(s)	172
Cac8I	GCNNGC	2 cut(s)	169, 197
CfoI	GCGC	1 cut(s)	870
Cfr13I	GGNCC	4 cut(s)	105, 106, 122, 769
Cfr9I	CCCGGG	1 cut(s)	108
CseI	GACGC	1 cut(s)	839
Csp6I	GTAC	3 cut(s)	264, 369, 516
CviAII	CATG	4 cut(s)	54, 226, 240, 812
CviQI	GTAC	3 cut(s)	264, 369, 516
DdeI	CTNAG	4 cut(s)	129, 349, 459, 885
DpnI	GATC	7 cut(s)	34, 59, 212, 230, 273, 555, 753
DpnII	GATC	7 cut(s)	32, 57, 210, 228, 271, 553, 751
DrdI	GACNNNNNNGTC	1 cut(s)	13
DseDI	GACNNNNNNGTC	1 cut(s)	13
Eam1104I	CTCTTC	1 cut(s)	741
EarI	CTCTTC	1 cut(s)	741
Eco130I	CCWWGG	1 cut(s)	48
Eco24I	GRGCYC	1 cut(s)	109
Eco47I	GGWCC	2 cut(s)	122, 769
Eco57I	CTGAAG	2 cut(s)	405, 858
Eco88I	CYCGRG	2 cut(s)	108, 401
EcoO109I	RGGNCCY	2 cut(s)	105, 769
EcoRI	GAATTC	1 cut(s)	678
EcoRII	CCWGG	1 cut(s)	189
EcoT14I	CCWWGG	1 cut(s)	48
EcoT22I	ATGCAT	1 cut(s)	550
EcoT38I	GRGCYC	1 cut(s)	109
ErhI	CCWWGG	1 cut(s)	48
FaeI	CATG	4 cut(s)	57, 229, 243, 815
FatI	CATG	4 cut(s)	53, 225, 239, 811
FauI	CCCGC	1 cut(s)	176
FblI	GTMKAC	2 cut(s)	5, 114
Fnu4HI	GCNGC	1 cut(s)	761
FokI	GGATG	1 cut(s)	452
FriOI	GRGCYC	1 cut(s)	109
Fsp4HI	GCNGC	1 cut(s)	761
FspBI	CTAG	2 cut(s)	674, 743
GlaI	GCGC	1 cut(s)	869
GluI	GCNGC	1 cut(s)	761
GsuI	CTGGAG	2 cut(s)	483, 776
HaeIII	GGCC	1 cut(s)	107
HapII	CCGG	5 cut(s)	78, 109, 139, 145, 361
HgaI	GACGC	1 cut(s)	839
HhaI	GCGC	1 cut(s)	870
Hin1I	GRCGYC	1 cut(s)	831
Hin1II	CATG	4 cut(s)	57, 229, 243, 815
Hin6I	GCGC	1 cut(s)	868
HinP1I	GCGC	1 cut(s)	868
HincII	GTYRAC	3 cut(s)	6, 808, 829
HindII	GTYRAC	3 cut(s)	6, 808, 829
HindIII	AAGCTT	1 cut(s)	483
HinfI	GANTC	6 cut(s)	69, 304, 353, 619, 646, 857
HpaII	CCGG	5 cut(s)	78, 109, 139, 145, 361
HphI	GGTGA	1 cut(s)	808
Hpy166II	GTNNAC	6 cut(s)	6, 64, 115, 266, 808, 829
Hpy188I	TCNGA	6 cut(s)	126, 210, 218, 352, 385, 684
Hpy188III	TCNNGA	9 cut(s)	30, 139, 232, 308, 403, 500, 636, 716, 755
Hpy8I	GTNNAC	6 cut(s)	6, 64, 115, 266, 808, 829
Hpy99I	CGWCG	5 cut(s)	10, 19, 22, 77, 155
HpyAV	CCTTC	1 cut(s)	787
HpyCH4III	ACNGT	4 cut(s)	323, 368, 654, 824
HpyCH4IV	ACGT	2 cut(s)	8, 527
HpyCH4V	TGCA	6 cut(s)	260, 490, 506, 521, 548, 739
HpyF10VI	GCNNNNNNNGC	5 cut(s)	83, 104, 173, 257, 503
HpyF3I	CTNAG	4 cut(s)	129, 349, 459, 885
HpySE526I	ACGT	2 cut(s)	8, 527
Hsp92I	GRCGYC	1 cut(s)	831
Hsp92II	CATG	4 cut(s)	57, 229, 243, 815
HspAI	GCGC	1 cut(s)	868
Kpn2I	TCCGGA	1 cut(s)	138
KpnI	GGTACC	1 cut(s)	372
Kzo9I	GATC	7 cut(s)	32, 57, 210, 228, 271, 553, 751
LmnI	GCTCC	4 cut(s)	141, 329, 502, 757
Lsp1109I	GCAGC	1 cut(s)	772
LweI	GCATC	3 cut(s)	165, 493, 535
MaeI	CTAG	2 cut(s)	674, 743
MaeII	ACGT	2 cut(s)	8, 527
MaeIII	GTNAC	2 cut(s)	317, 523
MalI	GATC	7 cut(s)	34, 59, 212, 230, 273, 555, 753
MboI	GATC	7 cut(s)	32, 57, 210, 228, 271, 553, 751
MboII	GAAGA	6 cut(s)	171, 418, 554, 758, 851, 862
MfeI	CAATTG	2 cut(s)	99, 390
MflI	RGATCY	4 cut(s)	32, 210, 553, 751
MhlI	GDGCHC	2 cut(s)	14, 109
MluCI	AATT	6 cut(s)	99, 390, 409, 474, 536, 678
MmeI	TCCRAC	1 cut(s)	149
MnlI	CCTC	9 cut(s)	34, 37, 212, 224, 294, 358, 456, 742, 808
Mph1103I	ATGCAT	1 cut(s)	550
MroI	TCCGGA	1 cut(s)	138
MseI	TTAA	5 cut(s)	480, 533, 627, 765, 801
MspCI	CTTAAG	1 cut(s)	764
MspI	CCGG	5 cut(s)	78, 109, 139, 145, 361
MspR9I	CCNGG	4 cut(s)	109, 110, 146, 191
MunI	CAATTG	2 cut(s)	99, 390
MvaI	CCWGG	1 cut(s)	191
MvnI	CGCG	1 cut(s)	870
MwoI	GCNNNNNNNGC	5 cut(s)	83, 104, 173, 257, 503
NciI	CCSGG	3 cut(s)	109, 110, 146
NdeII	GATC	7 cut(s)	32, 57, 210, 228, 271, 553, 751
NlaIII	CATG	4 cut(s)	57, 229, 243, 815
NlaIV	GGNNCC	6 cut(s)	106, 107, 123, 143, 370, 555
NmuCI	GTSAC	2 cut(s)	317, 523
NsiI	ATGCAT	1 cut(s)	550
PaeR7I	CTCGAG	1 cut(s)	401
PcsI	WCGNNNNNNNCGW	2 cut(s)	11, 14
PfeI	GAWTC	6 cut(s)	69, 304, 353, 619, 646, 857
PflMI	CCANNNNNTGG	1 cut(s)	54
PkrI	GCNGC	1 cut(s)	762
PpuMI	RGGWCCY	1 cut(s)	769
Psp5II	RGGWCCY	1 cut(s)	769
Psp6I	CCWGG	1 cut(s)	189
PspGI	CCWGG	1 cut(s)	189
PspN4I	GGNNCC	6 cut(s)	106, 107, 123, 143, 370, 555
PspOMI	GGGCCC	1 cut(s)	105
PspPI	GGNCC	4 cut(s)	105, 106, 122, 769
PspPPI	RGGWCCY	1 cut(s)	769
PstI	CTGCAG	1 cut(s)	523
PsuI	RGATCY	4 cut(s)	32, 210, 553, 751
RsaI	GTAC	3 cut(s)	265, 370, 517
RsaNI	GTAC	3 cut(s)	264, 369, 516
SalI	GTCGAC	1 cut(s)	4
SaqAI	TTAA	5 cut(s)	480, 533, 627, 765, 801
SatI	GCNGC	1 cut(s)	761
Sau3AI	GATC	7 cut(s)	32, 57, 210, 228, 271, 553, 751
Sau96I	GGNCC	4 cut(s)	105, 106, 122, 769
ScaI	AGTACT	1 cut(s)	517
ScrFI	CCNGG	4 cut(s)	109, 110, 146, 191
SduI	GDGCHC	2 cut(s)	14, 109
SfaNI	GCATC	3 cut(s)	165, 493, 535
SfcI	CTRYAG	1 cut(s)	519
Sfr274I	CTCGAG	1 cut(s)	401
SinI	GGWCC	2 cut(s)	122, 769
SlaI	CTCGAG	1 cut(s)	401
SmaI	CCCGGG	1 cut(s)	110
SmlI	CTYRAG	2 cut(s)	401, 764
SmoI	CTYRAG	2 cut(s)	401, 764
Sse9I	AATT	6 cut(s)	99, 390, 409, 474, 536, 678
SsiI	CCGC	3 cut(s)	86, 92, 169
SspI	AATATT	1 cut(s)	430
SspMI	CTAG	2 cut(s)	674, 743
StyD4I	CCNGG	4 cut(s)	107, 108, 144, 189
StyI	CCWWGG	1 cut(s)	48
TaaI	ACNGT	4 cut(s)	323, 368, 654, 824
TaiI	ACGT	2 cut(s)	11, 530
TaqI	TCGA	3 cut(s)	5, 402, 562
TasI	AATT	6 cut(s)	99, 390, 409, 474, 536, 678
TatI	WGTACW	2 cut(s)	263, 515
TfiI	GAWTC	6 cut(s)	69, 304, 353, 619, 646, 857
Tru1I	TTAA	5 cut(s)	480, 533, 627, 765, 801
Tru9I	TTAA	5 cut(s)	480, 533, 627, 765, 801
TscAI	CASTG	2 cut(s)	273, 513
TseFI	GTSAC	2 cut(s)	317, 523
TseI	GCWGC	1 cut(s)	760
Tsp45I	GTSAC	2 cut(s)	317, 523
TspDTI	ATGAA	2 cut(s)	659, 806
TspGWI	ACGGA	2 cut(s)	81, 486
TspMI	CCCGGG	1 cut(s)	108
TspRI	CASTG	2 cut(s)	273, 513
Van91I	CCANNNNNTGG	1 cut(s)	54
Vha464I	CTTAAG	1 cut(s)	764
VpaK11BI	GGWCC	2 cut(s)	122, 769
XapI	RAATTY	2 cut(s)	536, 678
XhoI	CTCGAG	1 cut(s)	401
XmaI	CCCGGG	1 cut(s)	108
XmiI	GTMKAC	2 cut(s)	5, 114
XspI	CTAG	2 cut(s)	674, 743
ZrmI	AGTACT	1 cut(s)	517
Zsp2I	ATGCAT	1 cut(s)	550

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.