Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0005721

Physical Location & Seq

Genomic Coordinates Forward (+)

36880999 .. 36889981

Gene Structure 8983 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1256000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5661 bp

ATGCGACTCTCGTCGAAGCAAAGTACGGTGTCGAATCGGTCAGGACCTCGGCTTAGGAAGAAGCACAAGAGGCTTGATGCTATATGTGAGCAGGAGTATAAGAGGAACCATAATGGTGGGGTGGGACCTGGGCCGGGGAGATCGGGGAGTGGTGAGGTGGAGCTGCGTCGGAGCGGGAGGGCTCGCCGTGCCCCGATTTTGCTTGATGTCTCTCCTTCACCGCCGAAGAAGCGGCGGAAGGTTGATAAGAATGTAGTGGTGGGTGCTGGGATGAGTGTGAAGGAGGAAGATTTGGAAACGCCGGGAAGTTGGAGATCGAGGTTGAGATCAAGGGGTAGAAATGTGGGTTTTGGGGTAAAAGGGAAACGGAAGCTTTATGAAGAGGTGAGTGAATGCCGAAGGGAAGAGAAGATGGTGGGGACTCGATCGAATGATAAGAATGGGGAATTTGAAAGTGGGAGGCATAGAGTTGTCAAATCAAAGGGACCAGGGAGGATTAGAGCAACAAATAGTTCAGACCATGAACTGAGAGAGGAATTGCCCGCGAATAAAGATGAACCTGTGGAGGAGGAAGCAGAGGTGATAATTAAGAATGAAGATGTTGCTATGCAATTGGATAGCGGAATGGATGGTGGCAAGGAAATGGAAATAGTGGACGATGATTCCACAATGATAACTGAGGCAGGGGAGAATTCAAAATTAGAGGAAGCATGCATTGTTAATGAGCATGTGGCAACAATGGACAATGCGGAGGTGATGGAACATGCTGATGACCTTGTTGCAACTGTGGACAATGTGGATACGATGGAACATGCTAATGAGCTTGTGGCAACTGTGGACAATGTGGAGACGATGGAACATGCTGATGAGCATGTGGAAGCTATGGACAATGTGGAGCCAACAGAACATGCTGATGAAAAGGTGGAGCAATCTGAGTGCGTGATTCAAGAAGAAAATAATGATAAGCATGTGAAACAGCTAGAGTGTGTGAAGGAAGGAGAAAATCAAGGGGATGCTAGGGTGTCAGAAGCTGTTAGAGTTTCAAGAAATGAAGTGGAAGTAGCAGGGTGTCATGAAGGGAAGGATTCTTATTCATCCAAGCCTGATGAAGAGCTTGCAATAGAAATGAATAATGCGAAGGTGGATAAGTTTAAACATAGGAAAAGTGATACACTTGGCAAGCCACACATTAAGGAGGGCAGAAGGTGTGGTTTGTGTGGGGGAGGGACTGATGGTAAACCTCCAAAAAGATTAGTTCATGATACGGGTGAGAGCGAGAATGAGGCATACTGTGGCTCTTCAGCCTCTGAGGAACTTAACTATAACCTATGGGATGGTTTTGGTGATGAACCTGGTTGGCTTGGACGTCTGTTGGGTCCTGTAAATGATCGTTATGGTATTGCCGGAATATGGGTCCATCAAAACTGTGCTGTGTGGAGTCCAGAGGTTTATTTTGCTGGTTTAGGATGCTTAAAAAATGTGAGGGCTGCACTATGCAGAGGGAGAGCATTAAAATGCACCCGCTGTGGAAGGGCAGGAGCTACAATTGGGTGCCGCGTTGATCGGTGTCCAAGAACTTACCACTTGCCTTGTGCACGAGCTTATGGTTGTGTATTTGATCATCGCAAATTTCTTATTGCCTGTACTGATCATCGGAATCTCTTCCAACCTCTTGGTGATCAATATTTAGCCCGTATAAAGAAATTAAAGGCTAAGAAGATGAAGATGGAAACGAGAAAACTTTCAAATGATGCTTTGCGGAAGGATATTGAGGCAGAAGAAAAATGGTTAGAGAATTGTGGTGAGGATGAAGAGTTTTTGAAACGTGAAAGTAAGAGGCTTCATCGGGATTTGGTGAGAATTGCACCCGTTTACATTGGAGGCTCCGACTCAGAGAGTGGAAAACTATTTCAGGGTTGGGAATCTGTTGCAGGCCTTCAAGATGTCATTGGGTGCATGAAGGAAGTTGTCATGTTGCCTCTTTTATATCCAGAGTTCTTTGATAGTCTTGGTCTCACACCTCCTAGAGGTGTTCTTTTGCATGGGTATCCTGGAACAGGTAAAACTCTTGTTGTCCGGGCATTAATAGGTGCTTGTGCACGTGGTGATAAACGGATAGCATATTTTGCTCGCAAAGGTGCAGATTGTCTAGGGAAATATGTAGGTGATTCAGAGCGCCAGCTAAGACTCTTGTTTCAGGTTGCTGAGAAATGTCAACCTTCTATAATATTCTTTGATGAGATAGATGGGTTGGCACCTTCTCGAACGAGGCAGCAAGATCAGACACATAGTTCAGTTGTGTCAACACTGCTTGCTTTAATGGATGGTTTGAAATCTCGAGGTTCAGTTGTAGTTATAGGCGCCACAAATCGTCCTGATGCTGTTGATCCAGCATTGAGGCGGCCCGGACGATTTGATAGGGAAATCTATTTTCCGCTGCCATCAGTGGAGGACCGAGCTGCCATTCTTTCACTTCACACTCAAAAGTGGCCAAAACCAGTTGCTGGATCCATCCTCAAGTTGGTTGCAAGCAGAACTGCTGGATTTGCTGGTGCAGATCTGCAGGCACTATGTACTCAAGCGGCTATCATTTCTTTGAAGAGAAATTTCCCTTTGCAAGAAGTTTTGTCTACCGCTGGAAAGAATGGTTCCGACCACAAGCGCCTCTCTCTTCCTGCCTTTGCAGTGGAGGACAGGGACTGGTTGGAAGCTCTCTCATGTTCTCCGCCTCCCTGCTCTCGTAGAGAAGCAGGAGTAGCTGCTAATGATGTAGTATGCTCTCCTCTTCCTACCCATCTTATCCCATGTCTGCTACAACCATTATCTACTATGCTTGTTTCCCTTTATCTTGATGAACGACTCTGGTTGCCGGCTCCTCTCAGAAAATCTGCAAGAATGATTGAAAGTGTGATGGTTTCTGCTTTGAATAAGAAGAAGATGCCCAGTGATCGTTGGTGGTCCCATATATCAGTCTTACTTCAGGAAGCAGATGTTGCAAAAGACATAGAGAGAAAGCTTTTGCGCACTGGCATTTTACTGGGATATGATACTTGTGCTGATTCTGATGTTTTTAATGACGATCATGATGATGACGACAGTTTGAAGTTTCAGCCTTCTGTTAAACACCATGGTGGCACACGTCCGAGTTTGTTGCGGAATATATCCGTCGCCTCAACCAGCAAATCAGGATTTCGGATATTGATTGCTGGAAATCCCAGAGCTGGGCAAAGGCATCTTGCTTCTTGCCTTCTTCATTGTTTTGTTGGAAATGTACAAGTTCAGAAGGTTGATTTGGCTACAGTTTTACAAGAAGGGCATGGAGATATGGTGCAAGGCATAACAAAAATATTAATGAAATGTGCTAGTGTTGGGTCATCTATAGTTTTCATGCCACGAATTGATTTGTGGGCTGTAGAGACACTTCTTCCAACGACAGAGGAAAGTGATTCCGACTTATCAAACCATCTACTTACTGAAAATGAAAAATCGTATTCTGTACATGGTCAATCTGTTGAAGAGGGGAGTGAGCCCACTTCACAGCAATGCAAATCTATAGAAATGGGAGAGTGCACAGGTGATTCTCAGGGCGCTTCACATGCCTGGAACCTATTTGTTGAGCAGGTGGAGTCTATTTGCGTCTCCTCATCCTTGATGATTCTGGCTACATCGGAAGTTCCATATTCAGTACTTCCAGTAAGAATAAGACAGTTCTTCAAGAGTGACATATCAAATTGCAATCAGTCAATTCCTATGAAGAATACAGTTCCCCGATTTTCTGTGCAGATTGATGAAGATTTCAACCATGATTTGGTGATCAATCTATCCACAGAAGAGTTATTAAGGGATATGGTTCAACAGGTTGTTCTGTTAATTCATCAAAAGTCCCACATCCATACAACTTCATACCAGGAGTGTGGAACTTGTGATCCTTGGGATCATCAATCAGAGATAGTTAATCACAGGACAGTTCATGGATCAGCTGATGTCAACAACAGTGTAACACAGGGTCCTAATGAATCTCTTCTTAAAGTTCATCTACCGCCTGACAATAGAAGTGTGAAGGGGAAGTCAAACTTGCTTCTTGCAATATCTTCATTTGGCTATCAAATTCTGCAATATCCTCATTTTGCTGAGCTTTGTTGGTTTACTTCAAAACTCAAAGAAGGTCCCTCTGCAGACATAAGTGGACCTTGGAAGGGCTGGCCATTCAATCCTTGTATAGTTCGACCTAATAACTCAATTGAAAAGGTAGCTGTTGCTTGTAGCTCCAGCAATACGAAAAGCAAAGAAAAATTTGGTCTAGTTAGAGGCCTTATTGCTGTTGGTTTATCGGCATATAGAGGTGTCTATACGTCACTCAGGGAAGTCTCCTTTGAGATACGGAAGGTTCTTGAGCTTCTAGTTGCCGAGGTCAATTCAAAGATTCAGGCTGGGAAAGATAGATATCAATATGTTCGCCTTTTGTCGCAAGTGGCCTATCTGGAAGACGTGGTTAATAGCTGGGCATACACGCTCCATAGTTTAGAGTTGGATGCTCCAATGAAAATGGAAAATGCTAAGCTAACTGATGTGAGACATCCAAAAGATGATGATGTGAGACCTCCAGATGATCACCATGTGGATGGACAAGTTCAAACTGAGGAACCCAAGCCAAATAATACTAGCAAATGCTCCGACGAGCTTGAAGTGCCGGAAAGTGTCCCTCAAGGGTTTGATACTGAAAAAGTCGGTTGTGTTGACTTAAACAAAGATGGTGAGTTGGGTCATCCTGATTCTGAGGGTAGACTTGCAATTTCAGATTTGTCTGGACAGAAAATCATCGTAATGGATTCTACCCTGGACAAATCTCTTCTTGGTTCCAGTGAAAGTTTGAATGAATCAGAAAATGACAGAAACCATGTCGAAGTGAATGGAGATTCTGGATCCTTGAAACATTCAAATGGATTTGACTGCACTGAATCTGTTGTCATTTCTGAGGATGGTTCTACTTCTGGTGAATTTGGCAGCATTAAGCTCTCAAGCTCTAGGGCCATCTGCAACGAGGTTAACGGTTTGTCCTCAATGGAGGCTGGTATTATACTTAATAATGGTAAGTGTGATGCCAATGAGCATATTGTAGGCATTGACACCTCGAACAAAATATTTCTCCCTACCAAGTCTGGGGTTCTTTGCTTGTATCGTTGTTGCCCTACATGCCTAGATACCCTCCGCAGTTTGACACATAAACTTCTTATTCACAAATGGGGATCAAATAAGAGCCAATGGACTATAGAGGATGCTCATGATATCGTTGCATCAGCGTCTGTTGATCTTCTTTCAGCCATTAGAAGAATTGTCGGTTCTGGAGGTTTCAGCAACTCACCGGATAATAAAATGAGAGACAGAAATAGTGAGGAGTTCAAATGGCCGGAAGCAGTTACATGTCACTGCAAAAATTCAGGAACTAAGAGTCTTTTGCCAGTGGAATGTAAATGCCACACCATCAGCGAGGACACATCCCTGAGTACTCATCGCAGGTTTGATCCAAAATTTATTTTCAGAGATGGTGTACTGATTCATATGGATCCTGACAAGGATGTTTCTTTCCACTGTAAATTCGAGACCCTGTGCCTCTGTTCTCTTATAGAGTTGATACTAATGGCCGAGCAGCCTTTTCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0003674 GO:0003682 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0005829 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010570 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0012505 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019898 GO:0031090 GO:0031312 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031935 GO:0031936 GO:0031984 GO:0033043 GO:0033044 GO:0040008 GO:0040029 GO:0042175 GO:0042393 GO:0042406 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045815 GO:0045892 GO:0045893 GO:0045927 GO:0045934 GO:0045935 GO:0045944 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070784 GO:0070786 GO:0080090 GO:0090033 GO:0098827 GO:1900428 GO:1900430 GO:1902275 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:2000112 GO:2000113 GO:2000217 GO:2000219 GO:2001141 GO:2001251

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA zf-HC5HC2H

Protein Analysis

1887

Amino Acids

208.78

Weight (kDa)

5.94

Isoelectric Point (pI)

46.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-HC5HC2H_2	PF13832	470 - 543	3.2e-08	PHD-zinc-finger like domain
zf-HC5HC2H	PF13771	473 - 552	6.5e-11	PHD-like zinc-binding domain
AAA	PF00004	678 - 813	2.4e-38	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	839 - 871	2.5e-08	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005721)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G15120 AT3G15120
fragaria_vesca	FvH4_3g19240 FvH4_3g19240 FvH4_3g19240 FvH4_3g19240 FvH4_3g19240 FvH4_3g19240
malus_domestica	MD11G1256000.v1.1
prunus_persica	Prupe.4G173800_v2.0.a1 Prupe.4G173800_v2.0.a1 Prupe.4G173800_v2.0.a1 Prupe.4G173800_v2.0.a1 Prupe.4G173800_v2.0.a1 Prupe.4G173800_v2.0.a1
pyrus_communis	pycom11g22640
rosa_chinensis	RchiOBHm_Chr5g0032311
rosa_laevigata	RLG00000033384
rosa_multiflora	Rmu_co8438997.1_g000001 Rmu_sc0002290.1_g000003
rosa_roxburghii	Rroxscaffold_1G00048110
rosa_rugosa	Rorug05G0130600 Rorug05G0130700
rosa_samantha	Rh5AG224200 Rh5BG225000 Rh5CG250400 Rh5DG229800
rosa_wichuraiana	Rw5G020480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3610
AatII	GACGTC	1 cut(s)	1369
Acc16I	TGCGCA	1 cut(s)	3023
Acc36I	ACCTGC	2 cut(s)	3610, 5505
AccB1I	GGYRCC	3 cut(s)	1551, 2254, 2360
AccB7I	CCANNNNNTGG	2 cut(s)	2504, 3808
AccBSI	CCGCTC	1 cut(s)	174
AccI	GTMKAC	2 cut(s)	2630, 4783
AccII	CGCG	2 cut(s)	545, 1557
AcoI	YGGCCR	4 cut(s)	2489, 4202, 5405, 5640
AcuI	CTGAAG	2 cut(s)	1284, 2963
AcvI	CACGTG	1 cut(s)	2102
AcyI	GRCGYC	2 cut(s)	1366, 2361
AdeI	CACNNNGTG	2 cut(s)	2105, 4618
AfaI	GTAC	8 cut(s)	25, 1645, 2575, 3273, 3498, 3687, 5506, 5550
AflIII	ACRYGT	2 cut(s)	3137, 5420
AjiI	CACGTC	2 cut(s)	3140, 4489
AjnI	CCWGG	7 cut(s)	127, 487, 1351, 2050, 3599, 3906, 4836
AjuI	GAANNNNNNNTTGG	2 cut(s)	3275, 3307
AleI	CACNNNNGTG	1 cut(s)	3129
AloI	GAACNNNNNNTCC	2 cut(s)	3910, 3942
Alw21I	GWGCWC	3 cut(s)	1597, 2101, 3572
Alw44I	GTGCAC	3 cut(s)	1593, 2097, 3568
AlwNI	CAGNNNCTG	5 cut(s)	1031, 1307, 2504, 2700, 5303
Ama87I	CYCGRG	1 cut(s)	2337
ApaLI	GTGCAC	3 cut(s)	1593, 2097, 3568
ApeKI	GCWGC	8 cut(s)	163, 1487, 2272, 2437, 2459, 2759, 5004, 5647
AseI	ATTAAT	2 cut(s)	2084, 3350
Asp700I	GAANNNNTTC	3 cut(s)	1661, 1741, 4020
AspLEI	GCGC	5 cut(s)	2178, 2363, 2664, 3024, 3590
AsuC2I	CCSGG	4 cut(s)	135, 303, 2078, 2406
AvaI	CYCGRG	1 cut(s)	2337
BaeGI	GKGCMC	4 cut(s)	193, 1597, 2101, 3572
BalI	TGGCCA	2 cut(s)	2491, 4204
BamHI	GGATCC	3 cut(s)	2507, 4922, 5563
BanI	GGYRCC	3 cut(s)	1551, 2254, 2360
BanII	GRGCYC	2 cut(s)	184, 3531
BarI	GAAGNNNNNNTAC	2 cut(s)	3405, 3437
BauI	CACGAG	1 cut(s)	1596
BbrPI	CACGTG	1 cut(s)	2102
BbsI	GAAGAC	1 cut(s)	4491
Bbv12I	GWGCWC	3 cut(s)	1597, 2101, 3572
BbvI	GCAGC	7 cut(s)	150, 1474, 2284, 2424, 2446, 2746, 5016
BceAI	ACGGC	1 cut(s)	171
BcgI	CGANNNNNNTGC	2 cut(s)	3133, 3167
BciT130I	CCWGG	7 cut(s)	129, 489, 1353, 2052, 3601, 3908, 4838
BciVI	GTATCC	2 cut(s)	793, 2058
BclI	TGATCA	5 cut(s)	1618, 1648, 1678, 3813, 4609
BcnI	CCSGG	4 cut(s)	135, 303, 2078, 2406
BfmI	CTRYAG	7 cut(s)	2561, 3297, 3378, 3411, 3552, 4173, 5268
BfoI	RGCGCY	4 cut(s)	2179, 2364, 2665, 3591
BfuAI	ACCTGC	2 cut(s)	3610, 5505
BfuI	GTATCC	2 cut(s)	793, 2058
BglII	AGATCT	1 cut(s)	2557
BlpI	GCTNAGC	2 cut(s)	4131, 4557
BmcAI	AGTACT	2 cut(s)	3687, 5506
BmeT110I	CYCGRG	1 cut(s)	2337
BmgBI	CACGTC	2 cut(s)	3140, 4489
BmrI	ACTGGG	2 cut(s)	2937, 3047
BmuI	ACTGGG	2 cut(s)	2937, 3047
BoxI	GACNNNNGTC	1 cut(s)	10
BpiI	GAAGAC	1 cut(s)	4491
BpmI	CTGGAG	3 cut(s)	4252, 4587, 5364
Bpu10I	CCTNAGC	1 cut(s)	53
Bpu1102I	GCTNAGC	2 cut(s)	4131, 4557
BpuEI	CTTGAG	5 cut(s)	2501, 2562, 4412, 4689, 5002
BpuMI	CCSGG	4 cut(s)	135, 303, 2078, 2406
BsaAI	YACGTR	1 cut(s)	2102
BsaBI	GATNNNNATC	1 cut(s)	4443
BsaHI	GRCGYC	2 cut(s)	1366, 2361
BsaI	GGTCTC	3 cut(s)	2018, 4591, 5594
BsaJI	CCNNGG	9 cut(s)	47, 128, 134, 488, 3127, 3929, 4190, 4407, 4836
BsaWI	WCCGGW	1 cut(s)	5362
BsaXI	ACNNNNNCTCC	2 cut(s)	3312, 3342
Bse118I	RCCGGY	1 cut(s)	2869
Bse1I	ACTGG	8 cut(s)	2498, 2705, 2943, 3031, 3042, 3692, 4860, 5459
Bse3DI	GCAATG	1 cut(s)	3548
Bse8I	GATNNNNATC	1 cut(s)	4443
BseBI	CCWGG	7 cut(s)	129, 489, 1353, 2052, 3601, 3908, 4838
BseDI	CCNNGG	9 cut(s)	47, 128, 134, 488, 3127, 3929, 4190, 4407, 4836
BseJI	GATNNNNATC	1 cut(s)	4443
BseMI	GCAATG	1 cut(s)	3548
BseNI	ACTGG	8 cut(s)	2498, 2705, 2943, 3031, 3042, 3692, 4860, 5459
BseRI	GAGGAG	5 cut(s)	581, 2772, 2865, 3631, 5408
BseSI	GKGCMC	4 cut(s)	193, 1597, 2101, 3572
BseXI	GCAGC	7 cut(s)	150, 1474, 2284, 2424, 2446, 2746, 5016
BseYI	CCCAGC	4 cut(s)	266, 3221, 4430, 4500
BsgI	GTGCAG	5 cut(s)	1473, 2160, 2574, 3800, 4936
Bsh1236I	CGCG	2 cut(s)	545, 1557
Bsh1285I	CGRYCG	1 cut(s)	428
BshNI	GGYRCC	3 cut(s)	1551, 2254, 2360
BsiEI	CGRYCG	1 cut(s)	428
BsiHKAI	GWGCWC	3 cut(s)	1597, 2101, 3572
BsiHKCI	CYCGRG	1 cut(s)	2337
BsiSI	CCGG	9 cut(s)	134, 302, 1404, 2077, 2406, 2870, 4691, 5363, 5408
BslFI	GGGAC	9 cut(s)	138, 433, 498, 1240, 2711, 2944, 3868, 4152, 4685
BsmBI	CGTCTC	2 cut(s)	842, 3643
BsmFI	GGGAC	9 cut(s)	138, 433, 498, 1240, 2711, 2944, 3868, 4152, 4685
BsmI	GAATGC	1 cut(s)	398
Bso31I	GGTCTC	3 cut(s)	2018, 4591, 5594
BsoBI	CYCGRG	1 cut(s)	2337
Bsp1286I	GDGCHC	6 cut(s)	184, 193, 1597, 2101, 3531, 3572
Bsp1407I	TGTACA	2 cut(s)	3271, 3496
Bsp1720I	GCTNAGC	2 cut(s)	4131, 4557
Bsp19I	CCATGG	1 cut(s)	3127
BspFNI	CGCG	2 cut(s)	545, 1557
BspHI	TCATGA	4 cut(s)	1072, 1258, 3082, 5281
BspMAI	CTGCAG	2 cut(s)	2565, 4177
BspMI	ACCTGC	2 cut(s)	3610, 5505
BspQI	GCTCTTC	2 cut(s)	1104, 1303
BspT107I	GGYRCC	3 cut(s)	1551, 2254, 2360
BspTNI	GGTCTC	3 cut(s)	2018, 4591, 5594
BsrBI	CCGCTC	1 cut(s)	174
BsrDI	GCAATG	1 cut(s)	3548
BsrFI	RCCGGY	1 cut(s)	2869
BsrGI	TGTACA	2 cut(s)	3271, 3496
BsrI	ACTGG	8 cut(s)	2498, 2705, 2943, 3031, 3042, 3692, 4860, 5459
BssAI	RCCGGY	1 cut(s)	2869
BssECI	CCNNGG	9 cut(s)	47, 128, 134, 488, 3127, 3929, 4190, 4407, 4836
BssNI	GRCGYC	2 cut(s)	1366, 2361
BssSI	CACGAG	1 cut(s)	1596
BssT1I	CCWWGG	3 cut(s)	3127, 3929, 4190
Bst2BI	CACGAG	1 cut(s)	1596
Bst2UI	CCWGG	7 cut(s)	129, 489, 1353, 2052, 3601, 3908, 4838
BstACI	GRCGYC	2 cut(s)	1366, 2361
BstAPI	GCANNNNNTGC	2 cut(s)	2126, 2993
BstAUI	TGTACA	2 cut(s)	3271, 3496
BstBAI	YACGTR	1 cut(s)	2102
BstDSI	CCRYGG	1 cut(s)	3127
BstENI	CCTNNNNNAGG	2 cut(s)	2025, 2055
BstFNI	CGCG	2 cut(s)	545, 1557
BstH2I	RGCGCY	4 cut(s)	2179, 2364, 2665, 3591
BstHHI	GCGC	5 cut(s)	2178, 2363, 2664, 3024, 3590
BstMCI	CGRYCG	1 cut(s)	428
BstNI	CCWGG	7 cut(s)	129, 489, 1353, 2052, 3601, 3908, 4838
BstPAI	GACNNNNGTC	1 cut(s)	10
BstSFI	CTRYAG	7 cut(s)	2561, 3297, 3378, 3411, 3552, 4173, 5268
BstSLI	GKGCMC	4 cut(s)	193, 1597, 2101, 3572
BstUI	CGCG	2 cut(s)	545, 1557
BstV1I	GCAGC	7 cut(s)	150, 1474, 2284, 2424, 2446, 2746, 5016
BstV2I	GAAGAC	1 cut(s)	4491
BstX2I	RGATCY	4 cut(s)	2507, 2557, 4922, 5563
BstXI	CCANNNNNNTGG	4 cut(s)	116, 1673, 3914, 4622
BstYI	RGATCY	4 cut(s)	2507, 2557, 4922, 5563
BsuI	GTATCC	2 cut(s)	793, 2058
BtgI	CCRYGG	1 cut(s)	3127
BtgZI	GCGATG	2 cut(s)	1607, 5495
BtrI	CACGTC	2 cut(s)	3140, 4489
BtsI	GCAGTG	3 cut(s)	2306, 2691, 5425
BveI	ACCTGC	2 cut(s)	3610, 5505
CaiI	CAGNNNCTG	5 cut(s)	1031, 1307, 2504, 2700, 5303
CciI	TCATGA	4 cut(s)	1072, 1258, 3082, 5281
CfoI	GCGC	5 cut(s)	2178, 2363, 2664, 3024, 3590
Cfr10I	RCCGGY	1 cut(s)	2869
CseI	GACGC	3 cut(s)	155, 3625, 5289
CsiI	ACCWGGT	1 cut(s)	1351
Csp6I	GTAC	8 cut(s)	24, 1644, 2574, 3272, 3497, 3686, 5505, 5549
CspCI	CAANNNNNGTGG	2 cut(s)	1571, 1606
CviQI	GTAC	8 cut(s)	24, 1644, 2574, 3272, 3497, 3686, 5505, 5549
DinI	GGCGCC	1 cut(s)	2362
DraI	TTTAAA	1 cut(s)	1153
DraIII	CACNNNGTG	2 cut(s)	2105, 4618
EaeI	YGGCCR	4 cut(s)	2489, 4202, 5405, 5640
EciI	GGCGGA	2 cut(s)	250, 2715
Eco130I	CCWWGG	3 cut(s)	3127, 3929, 4190
Eco147I	AGGCCT	2 cut(s)	1935, 4311
Eco24I	GRGCYC	2 cut(s)	184, 3531
Eco31I	GGTCTC	3 cut(s)	2018, 4591, 5594
Eco32I	GATATC	2 cut(s)	4445, 5287
Eco57I	CTGAAG	2 cut(s)	1284, 2963
Eco72I	CACGTG	1 cut(s)	2102
Eco88I	CYCGRG	1 cut(s)	2337
EcoNI	CCTNNNNNAGG	2 cut(s)	2025, 2055
EcoO109I	RGGNCCY	5 cut(s)	44, 125, 1376, 4007, 4166
EcoRI	GAATTC	1 cut(s)	691
EcoRII	CCWGG	7 cut(s)	127, 487, 1351, 2050, 3599, 3906, 4836
EcoRV	GATATC	2 cut(s)	4445, 5287
EcoT14I	CCWWGG	3 cut(s)	3127, 3929, 4190
EcoT22I	ATGCAT	1 cut(s)	716
EcoT38I	GRGCYC	2 cut(s)	184, 3531
EgeI	GGCGCC	1 cut(s)	2362
EheI	GGCGCC	1 cut(s)	2362
ErhI	CCWWGG	3 cut(s)	3127, 3929, 4190
Esp3I	CGTCTC	2 cut(s)	842, 3643
FalI	AAGNNNNNCTT	4 cut(s)	4058, 4090, 4174, 4206
FaqI	GGGAC	9 cut(s)	138, 433, 498, 1240, 2711, 2944, 3868, 4152, 4685
FauI	CCCGC	3 cut(s)	167, 550, 1529
FauNDI	CATATG	1 cut(s)	5559
FbaI	TGATCA	5 cut(s)	1618, 1648, 1678, 3813, 4609
FblI	GTMKAC	2 cut(s)	2630, 4783
FriOI	GRGCYC	2 cut(s)	184, 3531
FspI	TGCGCA	1 cut(s)	3023
GlaI	GCGC	5 cut(s)	2177, 2362, 2663, 3023, 3589
GsaI	CCCAGC	4 cut(s)	270, 3225, 4434, 4504
GsuI	CTGGAG	3 cut(s)	4252, 4587, 5364
HaeII	RGCGCY	4 cut(s)	2179, 2364, 2665, 3591
HapII	CCGG	9 cut(s)	134, 302, 1404, 2077, 2406, 2870, 4691, 5363, 5408
HgaI	GACGC	3 cut(s)	155, 3625, 5289
HhaI	GCGC	5 cut(s)	2178, 2363, 2664, 3024, 3590
Hin1I	GRCGYC	2 cut(s)	1366, 2361
Hin6I	GCGC	5 cut(s)	2176, 2361, 2662, 3022, 3588
HinP1I	GCGC	5 cut(s)	2176, 2361, 2662, 3022, 3588
HincII	GTYRAC	5 cut(s)	2216, 2304, 3988, 4738, 5047
HindII	GTYRAC	5 cut(s)	2216, 2304, 3988, 4738, 5047
HindIII	AAGCTT	2 cut(s)	371, 3014
HpaI	GTTAAC	1 cut(s)	5047
HpaII	CCGG	9 cut(s)	134, 302, 1404, 2077, 2406, 2870, 4691, 5363, 5408
Hpy99I	CGWCG	4 cut(s)	16, 171, 3170, 4679
HpyCH4IV	ACGT	6 cut(s)	1366, 1825, 2101, 3139, 4352, 4488
HpySE526I	ACGT	6 cut(s)	1366, 1825, 2101, 3139, 4352, 4488
Hsp92I	GRCGYC	2 cut(s)	1366, 2361
HspAI	GCGC	5 cut(s)	2176, 2361, 2662, 3022, 3588
KasI	GGCGCC	1 cut(s)	2360
KroI	GCCGGC	1 cut(s)	2869
KroNI	GCCGGC	1 cut(s)	2871
Ksp22I	TGATCA	5 cut(s)	1618, 1648, 1678, 3813, 4609
KspAI	GTTAAC	1 cut(s)	5047
LguI	GCTCTTC	2 cut(s)	1104, 1303
Lsp1109I	GCAGC	7 cut(s)	150, 1474, 2284, 2424, 2446, 2746, 5016
MabI	ACCWGGT	1 cut(s)	1351
MaeII	ACGT	6 cut(s)	1366, 1825, 2101, 3139, 4352, 4488
MaeIII	GTNAC	5 cut(s)	3719, 3997, 4353, 5416, 5423
MbiI	CCGCTC	1 cut(s)	174
MfeI	CAATTG	3 cut(s)	611, 1545, 4239
MflI	RGATCY	4 cut(s)	2507, 2557, 4922, 5563
MhlI	GDGCHC	6 cut(s)	184, 193, 1597, 2101, 3531, 3572
MlsI	TGGCCA	2 cut(s)	2491, 4204
MluNI	TGGCCA	2 cut(s)	2491, 4204
Mly113I	GGCGCC	1 cut(s)	2361
MlyI	GAGTC	7 cut(s)	415, 1447, 1883, 2181, 2853, 3635, 5458
Mox20I	TGGCCA	2 cut(s)	2491, 4204
Mph1103I	ATGCAT	1 cut(s)	716
MroNI	GCCGGC	1 cut(s)	2869
MroXI	GAANNNNTTC	3 cut(s)	1661, 1741, 4020
MscI	TGGCCA	2 cut(s)	2491, 4204
Msp20I	TGGCCA	2 cut(s)	2491, 4204
MspA1I	CMGCKG	4 cut(s)	1524, 2437, 2636, 3980
MspI	CCGG	9 cut(s)	134, 302, 1404, 2077, 2406, 2870, 4691, 5363, 5408
MssI	GTTTAAAC	1 cut(s)	1153
MunI	CAATTG	3 cut(s)	611, 1545, 4239
Mva1269I	GAATGC	1 cut(s)	398
MvaI	CCWGG	7 cut(s)	129, 489, 1353, 2052, 3601, 3908, 4838
MvnI	CGCG	2 cut(s)	545, 1557
NaeI	GCCGGC	1 cut(s)	2871
NarI	GGCGCC	1 cut(s)	2361
NciI	CCSGG	4 cut(s)	135, 303, 2078, 2406
NcoI	CCATGG	1 cut(s)	3127
NdeI	CATATG	1 cut(s)	5559
NgoMIV	GCCGGC	1 cut(s)	2869
NmeAIII	GCCGAG	2 cut(s)	28, 4432
NmuCI	GTSAC	3 cut(s)	3719, 4353, 5423
NsbI	TGCGCA	1 cut(s)	3023
NsiI	ATGCAT	1 cut(s)	716
OliI	CACNNNNGTG	1 cut(s)	3129
PaeI	GCATGC	1 cut(s)	714
PaeR7I	CTCGAG	1 cut(s)	2337
PagI	TCATGA	4 cut(s)	1072, 1258, 3082, 5281
PaqCI	CACCTGC	1 cut(s)	3610
PceI	AGGCCT	2 cut(s)	1935, 4311
PciI	ACATGT	1 cut(s)	5420
PciSI	GCTCTTC	2 cut(s)	1104, 1303
PctI	GAATGC	1 cut(s)	398
PdiI	GCCGGC	1 cut(s)	2871
PdmI	GAANNNNTTC	3 cut(s)	1661, 1741, 4020
PflMI	CCANNNNNTGG	2 cut(s)	2504, 3808
PfoI	TCCNGGA	1 cut(s)	2050
Ple19I	CGATCG	1 cut(s)	428
PleI	GAGTC	7 cut(s)	415, 1446, 1883, 2181, 2853, 3634, 5457
PluTI	GGCGCC	1 cut(s)	2364
PmaCI	CACGTG	1 cut(s)	2102
PmeI	GTTTAAAC	1 cut(s)	1153
PmlI	CACGTG	1 cut(s)	2102
PpsI	GAGTC	7 cut(s)	415, 1446, 1883, 2181, 2853, 3634, 5457
Ppu21I	YACGTR	1 cut(s)	2102
PpuMI	RGGWCCY	5 cut(s)	44, 125, 1376, 4007, 4166
PscI	ACATGT	1 cut(s)	5420
PshAI	GACNNNNGTC	1 cut(s)	10
PshBI	ATTAAT	2 cut(s)	2084, 3350
Psp5II	RGGWCCY	5 cut(s)	44, 125, 1376, 4007, 4166
Psp6I	CCWGG	7 cut(s)	127, 487, 1351, 2050, 3599, 3906, 4836
PspCI	CACGTG	1 cut(s)	2102
PspFI	CCCAGC	4 cut(s)	266, 3221, 4430, 4500
PspGI	CCWGG	7 cut(s)	127, 487, 1351, 2050, 3599, 3906, 4836
PspPPI	RGGWCCY	5 cut(s)	44, 125, 1376, 4007, 4166
PstI	CTGCAG	2 cut(s)	2565, 4177
PstNI	CAGNNNCTG	5 cut(s)	1031, 1307, 2504, 2700, 5303
PsuI	RGATCY	4 cut(s)	2507, 2557, 4922, 5563
PvuI	CGATCG	1 cut(s)	428
PvuII	CAGCTG	1 cut(s)	3980
RsaI	GTAC	8 cut(s)	25, 1645, 2575, 3273, 3498, 3687, 5506, 5550
RsaNI	GTAC	8 cut(s)	24, 1644, 2574, 3272, 3497, 3686, 5505, 5549
SapI	GCTCTTC	2 cut(s)	1104, 1303
ScaI	AGTACT	2 cut(s)	3687, 5506
SchI	GAGTC	7 cut(s)	415, 1447, 1883, 2181, 2853, 3635, 5458
SduI	GDGCHC	6 cut(s)	184, 193, 1597, 2101, 3531, 3572
SexAI	ACCWGGT	1 cut(s)	1351
SfcI	CTRYAG	7 cut(s)	2561, 3297, 3378, 3411, 3552, 4173, 5268
SfoI	GGCGCC	1 cut(s)	2362
Sfr274I	CTCGAG	1 cut(s)	2337
SlaI	CTCGAG	1 cut(s)	2337
SmlI	CTYRAG	6 cut(s)	2337, 2516, 2577, 4391, 4704, 5017
SmoI	CTYRAG	6 cut(s)	2337, 2516, 2577, 4391, 4704, 5017
SphI	GCATGC	1 cut(s)	714
SseBI	AGGCCT	2 cut(s)	1935, 4311
SspDI	GGCGCC	1 cut(s)	2360
SspI	AATATT	4 cut(s)	1685, 2229, 3348, 5142
StuI	AGGCCT	2 cut(s)	1935, 4311
StyI	CCWWGG	3 cut(s)	3127, 3929, 4190
TaiI	ACGT	6 cut(s)	1369, 1828, 2104, 3142, 4355, 4491
TaqII	GACCGA	2 cut(s)	27, 2469
TatI	WGTACW	7 cut(s)	1643, 2573, 3271, 3496, 3685, 5504, 5548
TauI	GCSGC	4 cut(s)	235, 1557, 2404, 2585
TseFI	GTSAC	3 cut(s)	3719, 4353, 5423
TseI	GCWGC	8 cut(s)	163, 1487, 2272, 2437, 2459, 2759, 5004, 5647
Tsp45I	GTSAC	3 cut(s)	3719, 4353, 5423
TspGWI	ACGGA	4 cut(s)	382, 2128, 3154, 4396
Van91I	CCANNNNNTGG	2 cut(s)	2504, 3808
VneI	GTGCAC	3 cut(s)	1593, 2097, 3568
VspI	ATTAAT	2 cut(s)	2084, 3350
XagI	CCTNNNNNAGG	2 cut(s)	2025, 2055
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2518
XhoI	CTCGAG	1 cut(s)	2337
XmiI	GTMKAC	2 cut(s)	2630, 4783
XmnI	GAANNNNTTC	3 cut(s)	1661, 1741, 4020
ZraI	GACGTC	1 cut(s)	1367
ZrmI	AGTACT	2 cut(s)	3687, 5506
Zsp2I	ATGCAT	1 cut(s)	716

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.