Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0005721

Physical Location & Seq

Genomic Coordinates Reverse (-)

26491095 .. 26500688

Gene Structure 9594 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000033384

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5571 bp

ATGCGATCATCGCCGAGTGGTGGTAAACAGTGGGGGCAGAGGAATCGGAAGAAGCACAAGAGGCTTGATGCTATTAGTGAAACCGTGTATAAGAGGAATCATCTTCAGGCTAATGATGGGGCGGGGCCGGGTCCCGGGAGCGGTGAATCGGAGCTTCGGAGGAGCTCCAGGGCACGCCGGGCCCCCGTTGTGCTTGATTTGTCGCCGGGGCCGCCGAAGAAGAGGAGGAGGGTTGGTAAGAATGGGAGATTGAGTGGAGGGAAGAATGTGAAGGAGGAGGAGGAGGATGAGGAGGAGGAGGAGGAGATGGAGGGAGGGTGGAAGTCGAGGTTGAGATCGAGGAGGAGGCATTCGGGGTTTGTGAAGGGGAAGAGGAAGCTTTTTGAAGATGTGAGTGGAGGTAGTAGTGGCGGGAAGGTAGTGGGGAGTGAGTTGGATGATGAGAATGAGGAAGGGTTGGAAGGTGGGAGGCCGAAGGTTGTGAAGTCGAAGAGACCAGGGAGGGTTAGAGCAACGCATGGTTCGGAGCATGAAGAGAATGAGTTGCTTGAAGTTAAGGAGGAAGTTGTGGGGGAAGAAGAGGAGGTGATGAGGGAGGACAGGGATAATTTAATGCAGTTGGATTGCGAAGAGGATGGTGGAATTGAGAGGGAAACTGTGGAAGGTGATTCCACAAAGATAATTGAGGCGGTGGGGGATTTACAGTTAGAGGAGGAGTGCACTGGCAATGAGAAAGTGGAAGCTGCTGAAACTGCAGATGGTGGGGAAATGATAGAACAAGTTGATGAACAGGTGGAGCAATCAGTTGATGTGATTGAAGAAGAAAAAGATGATGTGATTGAGGAAGAAAAAGATGATAAACAGATGGAACAAGAGCAAGTACCGTGTGTTGTTGAAGAAGAAGATCAAAGTGAGGCCATAGAAGCTGTTGGAGTCCCAGGAAATGAAGCAGAAGTTGAAGGGTGTCATGATGGGAAGGATTCACATTTGACCGTGCATGATGAAAAGCTTGCAATAGAAGTGAATACTGTGAAGGTGGATATGTCCGAAGATGGAAAGTCACAGATTAAGGAGGGCAGACGGTGTGGTTTGTGTGGGGGAGGGACTGACGGTATGCCTCCAAAAATATTAGCTCAGGATACAGTTGACATTGAGAATGAAGCATATAGTGGGTCTTCAGCCTCTGAGAAATTTGACTATAACATATGGGATGGTTTTGGTGATGAACCTGGATGGCTTGGACGTCTCTTGGGTCCAATAATTGATCGTTATGGTATTGCTGGAACATGGGTTCATCAACACTGTGCTGTATGGAGTCCAGAGGTTTACTTTGCTGGTGTAGGATGCTTAAAAAATATAAGGGCCGCTCTGTGCAGAGGGAGAGCGTTAAAATGCACCCGCTGTGGGAGGCCGGGAGCAACTATTGGATGCCGTGTTGATCGGTGTTCAAGAACTTACCACTTGCCTTGTGCACGAGCTATGAATTGCGTATTTGATCATCGCAAGTTTCTTATTGCCTGTACTGGTCATAGGAAACTCTTCCAGCCTCTTGGTTATCAGTACTTAGCTCGCAAAAAGAAATTGAAGGTTAAGAAAATGAAGTTCGAAATCAGAAAGCTATCAAATGATGCTTTGCGAAAGGATATTGAGGCAGAAGAAAAGTGGTTGGAGAATTGTGGCGATGATGAAGAGTTTTTGAAACGTGAAAGCAAGAGGCTACATCGAGATTTGGTGAGAATTGCACCTGTGTACATTGGAGGCTCCGACTCTGAGAGTGGCAAACTGTTTCAGGGTTGGGAATCTGTTGCAGGTCTTCAAAATGTGATTAGATGCATGAAGGAAGTTGTCATATTACCTCTTCTATATCCAGAGTTCTTTGATAGTCTCAGCCTCACACCTCCTAGAGGTGTTCTATTGCATGGGTATCCTGGAACAGGTAAAACTCTTGTTGTGCGGGCATTAATAGGTGCATGTGCACGTGGTGATAGACGAATAGCATATTTTGCTCGTAAAGGTGCTGATTGTTTAGGAAAATATGTTGGTGATGCTGAGCGCCAGCTAAGACTCTTGTTTCAGGTTGCTGAGAGATGTCAACCTTCTATAATATTCTTTGATGAGATTGATGGGTTGGCACCTTCCAGAACTAGGCAACAAGATCAGACACATAGTTCAGTTGTGTCAACACTGCTTGCTTTAATGGATGGTTTGAAATCTCGAGGTTCTGTTGTAGTGATAGGTGCTACAAATCGTCCTGATGCCGTTGATCCAGCACTAAGGCGGCCTGGAAGATTTGATAGGGAAATCTATTTTCCACTACCGTCAGAAGAGGACAGAGCTTCCATTCTTTCTGTTCATACTCAAAAATGGCCCAAACCAGTCAGTGGATCCGTGCTTAAGTCGATTGCAAGAGGAACTGCTGGATTTGCTGGTGCAGATCTGCAGGCACTTTGCACTCAAGCAGCCATAATTTCTTTGAAAAGGAATTTCCCTTTGCAAGAAGTTTTGTCTGCTGCTGGAAAGAAAGATTCTGAGTCCAAACGCCTCCCTCTACCTGCCTTTGTAGTGGAGGACAGGGATTGGTTGGAGGCTCTGACATGTTCTCCACCTCCCTGCTCTCGTAGGGAAGTAGGAATAGCTGCTAATGAAGTAGTATGCTCTCCTCTTCCAACCCACCTTATCCCATGTCTTCTACAACCATTATCTACTATGCTTGTATCCCTTTATCTTGATGAACGGCTCTGGTTGCCAACTCCTCTGAGAAGAGCTGCAACAATGATTGAAAGTGTGGTGGTTTCTGCCTTGAATAGGAAAAATATGCTCAGTGATCGCTGGTGGTCCCATATTGATGTTTTACTTCAGGAAGCAGATGTTGCAAAGGACATAGAGAGAAAGCTTTTGCATGCTGGAATCTTACATGGAGATACTGCTCTTGCTGATTCTGATGTTTGCAATGATGATGTTGATGACGGAATTTCGAATTTTGAGCCTTCTGTTAAACACCATGGTGGTACTCGCCCAAGTTTGTTGCAAAACACAGTAGTTGCCTCAACGTACAAACCGGGATTTCGGATATTGATTGCTGGAAATCCCAGATCTGGCCAAAGGCATCTTGCTTCCTGCCTTCTTCACTGTTTTGTTGGGAATGTAGAAATCCAGAAGGTTGATTTGGCTACAGTTTTGCTAGAAGGACACGGAGATATGGCGCAAGGGATAACACAAATATTAAAGAAATGTGCCAGCATGGGGTCGTGTATAGTTTTCATGCCAAGAATCGATTTGTGGGCAGTGGAGACACCTAATCAAGTAACAGAAGACAGTGATGCAGATTCATCAAACCATCAATGTCCTGAAAATGGAAAGCATCAACATCCTGAGAATGAAAAGTCATATTTTGCACATGGTCTGGAGGAAAGTGTGTCCTTATCACAGCAATGCAAATCAGAAGAAATGTTAGAGTGCCAAGATGATGTTGCTCAGAGTGCTTCACATGCTTGGAACTTGTTTGTGGAGCAGGTGGAGTCTCTAAGTGTCTCTGCATCCTTAATGATTCTGGCTACATCAGAAGTTCCATGTCCAGTGCTTCCGGTTAGAATAAGGCAGTTCTTTAAGAGTGACATTTTAAATGGCCATCGGTCAGTTCCTGTGAAGCATACAGTTCCCAGGTTTTCTGTGCAGGTTGATGGAAATTTCAACCACGATCTGGTGATCAATCTATCTTCAGCAGAGTTATCAAGGGATCTCGTTCAACAGATTGTTCTGTTAGTTCATCAAACTTCTCACATTCATACAACTTCATGCAAGGAGAGAGACTTTCACGGTATAAATGGTCAGTCCAAGGTGGTTGATCACAGTGCAGATCATGGATCAGCTGATGCAAACAACAATTTAACTCTACCTCCTGTTGAGTCTCTTCTTAAAGCTTCTGCACCACCTAACAATATAACTGGAAATGGGAAATCAAGCCTTCTGCTAGCAATATCTTCATTTGGCTATCAGATACTGCGATATCCTCATTTTGCTGAACTTTGTTGGTTCACTTCAAAGCTTAAAGAAGGTCCTTCTGCAGACATAAGTGGACCTTGGAAGGGTTGGCCATTCAATTCCTGTATAGTTCGTCCTAACAACTCGCTAGAAATGGTAGCTGTGGCAAAGAATATTAAAAGCAAAGAAAACTTGGGCCTAGTTAGAGGCCTGATTGCTGTTGGTTTATCGGCGTATAGAGGTCTCTATACATCTCTCAGAGAAGTCTCTTCTGAGGTACGGAAGGTTCTTGAGATTCTAGTTGCGCAGATCAATGCAAAAGTTCAAGCTGGGAAAGATAGATATCAATATGTTCGCCTTTTGTCGCAAGTGGCCTATCTGGAAGATGTGGTCAATAGCTGGGCATACACCTTGCAGAGTTTAGAGCAGGATGCTCCATTGGCAGATGCTAAGCTTACCGCTCTGAGACTTCCAGATGATAACCATATGGATGATCAAGTTCAAAGCGAGGATTCCAAGCCAAAGGTTACAAGCAAATGCTCCAATGAGCACGAAGTACTGGAAATTGTCCCTGAAAGGTTTGATGCTGAAAAAGTTGTCAGTATTGACGTGAATGAACAAGTTGCTGATTTGGGTTGTTCTGAGGTTAGACTTGCAAGTTTAGATTGTTCTGGACAGAAGATTGTCCCATCTGATTCTACCCTGGATAATTGTCTTAAAGATTCCGATGAAGTTTGGAATGACCAGAATGGGACAAACCCTAGACCCCACGAGTCAGAAAATGGCAAACACCATACAGTTGTTGCAGGAGATTCTGAATCTTTGAAACGTCCAAATGGATTTGAATGCACTGAATCTGTTGCTATTCCCGAGGATAGTCCGACTTCTGATACATTTGGCAGCATTAAGCTCTCTAGTTCTTTGACCAGTTACAACAACCTTAATGGTTTGTCCTTGACAGAGGCTGGTAGTAGACATAATGATGGTAAGGCTGATGCTGATGAGGTTACTGTAGATGTCAACTTCCCAAGCGCCACCAGTAATACCAATCTGCCTGAGTGCATAGATAAGCCTGATGCAAACTTCTCAAGTAAAACCAATGCTGCTGTGCATAATGTTGATGTCAACTTCCCTCCCAAAATCAGTCCCTCTACGAAGTCTGAGTTTGTATGCGTATATCATTGTTGCTCTGAATGCCTAGATACCCTCCACAGTTTGACACAGAAAATTCTTATCGAGGAGTGGCGATTAAATGGGAGCCAATGGACAGTGGAGGATGTTCATGATATTGTGGCATCACTATCTGTGGATCTTCTTTCAGCGGTTAGAAGAATTCATGTTTCTGGAGGTTTCAGCAATTCATTTGATGATAAAAGGAGACACAGAAATACTGAAACATATGAGTGGCCGGAATCAGGAACATGTGATTGCAATATCTCAGAAAATAAGATTCTTTCACCTGCTGAATGTAGATGCCACACCAGCAGGGAGAGCTTTACTCAAAAGGCAAATGCCTCCGCAAATACTCATCTCAGCTTGGATTGCCTAACCATGGCAAAATTTTGTGCTGTTGCGGGAATTTGGACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0003674 GO:0003682 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0005829 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010570 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0012505 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019898 GO:0031090 GO:0031312 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031935 GO:0031936 GO:0031984 GO:0033043 GO:0033044 GO:0040008 GO:0040029 GO:0042175 GO:0042393 GO:0042406 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045815 GO:0045892 GO:0045893 GO:0045927 GO:0045934 GO:0045935 GO:0045944 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070784 GO:0070786 GO:0080090 GO:0090033 GO:0098827 GO:1900428 GO:1900430 GO:1902275 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:2000112 GO:2000113 GO:2000217 GO:2000219 GO:2001141 GO:2001251

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA zf-HC5HC2H

Protein Analysis

1857

Amino Acids

204.85

Weight (kDa)

5.46

Isoelectric Point (pI)

50.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-HC5HC2H_2	PF13832	428 - 502	1.8e-08	PHD-zinc-finger like domain
zf-HC5HC2H	PF13771	432 - 510	2e-10	PHD-like zinc-binding domain
AAA	PF00004	637 - 772	1.3e-37	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	798 - 830	5.6e-08	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005721)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G15120 AT3G15120
fragaria_vesca	FvH4_3g19240 FvH4_3g19240 FvH4_3g19240 FvH4_3g19240 FvH4_3g19240 FvH4_3g19240
malus_domestica	MD11G1256000.v1.1
prunus_persica	Prupe.4G173800_v2.0.a1 Prupe.4G173800_v2.0.a1 Prupe.4G173800_v2.0.a1 Prupe.4G173800_v2.0.a1 Prupe.4G173800_v2.0.a1 Prupe.4G173800_v2.0.a1
pyrus_communis	pycom11g22640
rosa_chinensis	RchiOBHm_Chr5g0032311
rosa_laevigata	RLG00000033384
rosa_multiflora	Rmu_co8438997.1_g000001 Rmu_sc0002290.1_g000003
rosa_roxburghii	Rroxscaffold_1G00048110
rosa_rugosa	Rorug05G0130600 Rorug05G0130700
rosa_samantha	Rh5AG224200 Rh5BG225000 Rh5CG250400 Rh5DG229800
rosa_wichuraiana	Rw5G020480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	3502, 5449
AatII	GACGTC	1 cut(s)	1246
Acc16I	TGCGCA	1 cut(s)	4287
Acc36I	ACCTGC	5 cut(s)	1799, 2560, 3502, 3664, 5449
AccB1I	GGYRCC	1 cut(s)	2131
AccB7I	CCANNNNNTGG	2 cut(s)	2381, 3700
AccBSI	CCGCTC	3 cut(s)	141, 1367, 4442
AccI	GTMKAC	1 cut(s)	4954
AclWI	GGATC	6 cut(s)	2258, 2379, 2392, 3744, 3871, 5298
AcoI	YGGCCR	4 cut(s)	3097, 3625, 4091, 5387
AcsI	RAATTY	9 cut(s)	1190, 2482, 2970, 2977, 3685, 5208, 5313, 5540, 5559
AcuI	CTGAAG	4 cut(s)	89, 1161, 2840, 3702
AcvI	CACGTG	1 cut(s)	1979
AcyI	GRCGYC	1 cut(s)	1243
AdeI	CACNNNGTG	1 cut(s)	1982
AfaI	GTAC	8 cut(s)	882, 1522, 1562, 1751, 3010, 3053, 4260, 4539
AflII	CTTAAG	1 cut(s)	2393
AflIII	ACRYGT	2 cut(s)	2594, 5402
AjiI	CACGTC	2 cut(s)	4591, 5568
AjnI	CCWGG	8 cut(s)	167, 496, 937, 1228, 1927, 2281, 3659, 4683
AjuI	GAANNNNNNNTTGG	2 cut(s)	3149, 3181
AleI	CACNNNNGTG	1 cut(s)	3003
AloI	GAACNNNNNNTCC	2 cut(s)	1275, 1307
Alw21I	GWGCWC	5 cut(s)	167, 722, 1474, 1978, 4533
Alw44I	GTGCAC	3 cut(s)	718, 1470, 1974
AlwI	GGATC	6 cut(s)	2258, 2379, 2392, 3744, 3871, 5298
AlwNI	CAGNNNCTG	4 cut(s)	1184, 3641, 4000, 4946
Ama87I	CYCGRG	3 cut(s)	134, 2214, 4850
ApaI	GGGCCC	1 cut(s)	184
ApaLI	GTGCAC	3 cut(s)	718, 1470, 1974
ApeKI	GCWGC	7 cut(s)	743, 2459, 2510, 2636, 2765, 4881, 5084
ApoI	RAATTY	9 cut(s)	1190, 2482, 2970, 2977, 3685, 5208, 5313, 5540, 5559
AseI	ATTAAT	1 cut(s)	1961
Asp700I	GAANNNNTTC	1 cut(s)	1538
AspLEI	GCGC	4 cut(s)	2055, 3205, 4288, 5015
AsuC2I	CCSGG	7 cut(s)	129, 135, 136, 179, 207, 1413, 3060
AsuHPI	GGTGA	9 cut(s)	155, 598, 677, 1232, 1744, 1994, 2054, 3715, 5430
AsuII	TTCGAA	2 cut(s)	1605, 2975
AsuNHI	GCTAGC	1 cut(s)	3970
AvaI	CYCGRG	3 cut(s)	134, 2214, 4850
AvaII	GGWCC	5 cut(s)	131, 1253, 2835, 4055, 4076
BaeGI	GKGCMC	5 cut(s)	175, 184, 722, 1474, 1978
BalI	TGGCCA	3 cut(s)	3099, 3627, 4093
BamHI	GGATCC	1 cut(s)	2384
BanI	GGYRCC	1 cut(s)	2131
BanII	GRGCYC	2 cut(s)	167, 184
BauI	CACGAG	2 cut(s)	1473, 4751
BbrPI	CACGTG	1 cut(s)	1979
BbsI	GAAGAC	4 cut(s)	1167, 1805, 2678, 3318
Bbv12I	GWGCWC	5 cut(s)	167, 722, 1474, 1978, 4533
BbvI	GCAGC	7 cut(s)	730, 2471, 2497, 2623, 2752, 4893, 5071
BceAI	ACGGC	3 cut(s)	1416, 2243, 2750
BcgI	CGANNNNNNTGC	2 cut(s)	26, 60
BciT130I	CCWGG	8 cut(s)	169, 498, 939, 1230, 1929, 2283, 3661, 4685
BciVI	GTATCC	3 cut(s)	1132, 1935, 2725
BclI	TGATCA	4 cut(s)	1495, 3705, 3844, 4474
BcnI	CCSGG	7 cut(s)	129, 135, 136, 179, 207, 1413, 3060
BfmI	CTRYAG	5 cut(s)	753, 2438, 3171, 4062, 4992
BfoI	RGCGCY	2 cut(s)	2056, 5016
BfrI	CTTAAG	1 cut(s)	2393
BfuAI	ACCTGC	5 cut(s)	1799, 2560, 3502, 3664, 5449
BfuI	GTATCC	3 cut(s)	1132, 1935, 2725
BglII	AGATCT	2 cut(s)	2434, 3092
BlpI	GCTNAGC	2 cut(s)	2049, 4431
BmcAI	AGTACT	2 cut(s)	1562, 4539
Bme18I	GGWCC	5 cut(s)	131, 1253, 2835, 4055, 4076
BmeT110I	CYCGRG	3 cut(s)	134, 2214, 4850
BmgBI	CACGTC	2 cut(s)	4591, 5568
BmtI	GCTAGC	1 cut(s)	3974
BpiI	GAAGAC	4 cut(s)	1167, 1805, 2678, 3318
BplI	GAGNNNNNCTC	2 cut(s)	5464, 5496
BpmI	CTGGAG	3 cut(s)	151, 3425, 5346
Bpu10I	CCTNAGC	1 cut(s)	1134
Bpu1102I	GCTNAGC	2 cut(s)	2049, 4431
Bpu14I	TTCGAA	2 cut(s)	1605, 2975
BpuEI	CTTGAG	3 cut(s)	2439, 4292, 5053
BpuMI	CCSGG	7 cut(s)	129, 135, 136, 179, 207, 1413, 3060
Bsa29I	ATCGAT	1 cut(s)	3273
BsaAI	YACGTR	1 cut(s)	1979
BsaBI	GATNNNNATC	1 cut(s)	4323
BsaHI	GRCGYC	1 cut(s)	1243
BsaI	GGTCTC	2 cut(s)	487, 4229
BsaWI	WCCGGW	1 cut(s)	3583
Bse1I	ACTGG	8 cut(s)	727, 1528, 2375, 3575, 3949, 4545, 4908, 5019
Bse3DI	GCAATG	3 cut(s)	733, 2956, 3437
Bse8I	GATNNNNATC	1 cut(s)	4323
BseBI	CCWGG	8 cut(s)	169, 498, 939, 1230, 1929, 2283, 3661, 4685
BseCI	ATCGAT	1 cut(s)	3273
BseJI	GATNNNNATC	1 cut(s)	4323
BseMI	GCAATG	3 cut(s)	733, 2956, 3437
BseNI	ACTGG	8 cut(s)	727, 1528, 2375, 3575, 3949, 4545, 4908, 5019
BseSI	GKGCMC	5 cut(s)	175, 184, 722, 1474, 1978
BseXI	GCAGC	7 cut(s)	730, 2471, 2497, 2623, 2752, 4893, 5071
BseYI	CCCAGC	2 cut(s)	4310, 4380
BsgI	GTGCAG	5 cut(s)	1393, 2451, 3692, 3873, 3909
BshNI	GGYRCC	1 cut(s)	2131
BshVI	ATCGAT	1 cut(s)	3273
BsiHKAI	GWGCWC	5 cut(s)	167, 722, 1474, 1978, 4533
BsiHKCI	CYCGRG	3 cut(s)	134, 2214, 4850
BsiSI	CCGG	8 cut(s)	128, 135, 178, 206, 1412, 3059, 3584, 5390
BslFI	GGGAC	8 cut(s)	117, 920, 1117, 2821, 4535, 4652, 4747, 5112
BsmBI	CGTCTC	1 cut(s)	1250
BsmFI	GGGAC	8 cut(s)	117, 920, 1117, 2821, 4535, 4652, 4747, 5112
BsmI	GAATGC	3 cut(s)	349, 4832, 5180
Bso31I	GGTCTC	2 cut(s)	487, 4229
BsoBI	CYCGRG	3 cut(s)	134, 2214, 4850
Bsp119I	TTCGAA	2 cut(s)	1605, 2975
Bsp120I	GGGCCC	1 cut(s)	180
Bsp1286I	GDGCHC	7 cut(s)	167, 175, 184, 722, 1474, 1978, 4533
Bsp1407I	TGTACA	1 cut(s)	1749
Bsp1720I	GCTNAGC	2 cut(s)	2049, 4431
Bsp19I	CCATGG	2 cut(s)	3001, 5532
BspDI	ATCGAT	1 cut(s)	3273
BspHI	TCATGA	2 cut(s)	967, 5263
BspMAI	CTGCAG	3 cut(s)	757, 2442, 4066
BspMI	ACCTGC	5 cut(s)	1799, 2560, 3502, 3664, 5449
BspOI	GCTAGC	1 cut(s)	3974
BspPI	GGATC	6 cut(s)	2258, 2379, 2392, 3744, 3871, 5298
BspQI	GCTCTTC	1 cut(s)	2755
BspT104I	TTCGAA	2 cut(s)	1605, 2975
BspT107I	GGYRCC	1 cut(s)	2131
BspTI	CTTAAG	1 cut(s)	2393
BspTNI	GGTCTC	2 cut(s)	487, 4229
BsrBI	CCGCTC	3 cut(s)	141, 1367, 4442
BsrDI	GCAATG	3 cut(s)	733, 2956, 3437
BsrGI	TGTACA	1 cut(s)	1749
BsrI	ACTGG	8 cut(s)	727, 1528, 2375, 3575, 3949, 4545, 4908, 5019
BssNI	GRCGYC	1 cut(s)	1243
BssSI	CACGAG	2 cut(s)	1473, 4751
BssT1I	CCWWGG	4 cut(s)	3001, 3834, 4079, 5532
Bst2BI	CACGAG	2 cut(s)	1473, 4751
Bst2UI	CCWGG	8 cut(s)	169, 498, 939, 1230, 1929, 2283, 3661, 4685
BstACI	GRCGYC	1 cut(s)	1243
BstAFI	CTTAAG	1 cut(s)	2393
BstAPI	GCANNNNNTGC	2 cut(s)	2003, 2870
BstAUI	TGTACA	1 cut(s)	1749
BstBAI	YACGTR	1 cut(s)	1979
BstBI	TTCGAA	2 cut(s)	1605, 2975
BstDSI	CCRYGG	2 cut(s)	3001, 5532
BstENI	CCTNNNNNAGG	3 cut(s)	1902, 1932, 2803
BstH2I	RGCGCY	2 cut(s)	2056, 5016
BstHHI	GCGC	4 cut(s)	2055, 3205, 4288, 5015
BstNI	CCWGG	8 cut(s)	169, 498, 939, 1230, 1929, 2283, 3661, 4685
BstNSI	RCATGY	5 cut(s)	1974, 2598, 2903, 3491, 5406
BstSFI	CTRYAG	5 cut(s)	753, 2438, 3171, 4062, 4992
BstSLI	GKGCMC	5 cut(s)	175, 184, 722, 1474, 1978
BstV1I	GCAGC	7 cut(s)	730, 2471, 2497, 2623, 2752, 4893, 5071
BstV2I	GAAGAC	4 cut(s)	1167, 1805, 2678, 3318
BstX2I	RGATCY	5 cut(s)	2384, 2434, 3092, 3736, 5290
BstXI	CCANNNNNNTGG	1 cut(s)	1550
BstYI	RGATCY	5 cut(s)	2384, 2434, 3092, 3736, 5290
Bsu15I	ATCGAT	1 cut(s)	3273
BsuI	GTATCC	3 cut(s)	1132, 1935, 2725
BsuTUI	ATCGAT	1 cut(s)	3273
BtgI	CCRYGG	2 cut(s)	3001, 5532
BtgZI	GCGATG	2 cut(s)	1484, 1695
BtrI	CACGTC	2 cut(s)	4591, 5568
BtsI	GCAGTG	2 cut(s)	2183, 3291
BveI	ACCTGC	5 cut(s)	1799, 2560, 3502, 3664, 5449
CaiI	CAGNNNCTG	4 cut(s)	1184, 3641, 4000, 4946
CciI	TCATGA	2 cut(s)	967, 5263
CfoI	GCGC	4 cut(s)	2055, 3205, 4288, 5015
Cfr9I	CCCGGG	1 cut(s)	134
ClaI	ATCGAT	1 cut(s)	3273
Csp6I	GTAC	8 cut(s)	881, 1521, 1561, 1750, 3009, 3052, 4259, 4538
CspCI	CAANNNNNGTGG	2 cut(s)	1448, 1483
CviQI	GTAC	8 cut(s)	881, 1521, 1561, 1750, 3009, 3052, 4259, 4538
DraI	TTTAAA	1 cut(s)	3621
DraIII	CACNNNGTG	1 cut(s)	1982
EaeI	YGGCCR	4 cut(s)	3097, 3625, 4091, 5387
Ecl136II	GAGCTC	1 cut(s)	165
Eco130I	CCWWGG	4 cut(s)	3001, 3834, 4079, 5532
Eco147I	AGGCCT	1 cut(s)	4191
Eco24I	GRGCYC	2 cut(s)	167, 184
Eco31I	GGTCTC	2 cut(s)	487, 4229
Eco32I	GATATC	2 cut(s)	4007, 4325
Eco47I	GGWCC	5 cut(s)	131, 1253, 2835, 4055, 4076
Eco53kI	GAGCTC	1 cut(s)	165
Eco57I	CTGAAG	4 cut(s)	89, 1161, 2840, 3702
Eco72I	CACGTG	1 cut(s)	1979
Eco88I	CYCGRG	3 cut(s)	134, 2214, 4850
EcoICRI	GAGCTC	1 cut(s)	165
EcoNI	CCTNNNNNAGG	3 cut(s)	1902, 1932, 2803
EcoO109I	RGGNCCY	3 cut(s)	131, 181, 4055
EcoRI	GAATTC	1 cut(s)	5313
EcoRII	CCWGG	8 cut(s)	167, 496, 937, 1228, 1927, 2281, 3659, 4683
EcoRV	GATATC	2 cut(s)	4007, 4325
EcoT14I	CCWWGG	4 cut(s)	3001, 3834, 4079, 5532
EcoT22I	ATGCAT	1 cut(s)	1835
EcoT38I	GRGCYC	2 cut(s)	167, 184
ErhI	CCWWGG	4 cut(s)	3001, 3834, 4079, 5532
Esp3I	CGTCTC	1 cut(s)	1250
FalI	AAGNNNNNCTT	2 cut(s)	4063, 4095
FaqI	GGGAC	8 cut(s)	117, 920, 1117, 2821, 4535, 4652, 4747, 5112
FauI	CCCGC	5 cut(s)	115, 404, 1406, 1947, 5548
FauNDI	CATATG	3 cut(s)	1205, 4467, 5380
FbaI	TGATCA	4 cut(s)	1495, 3705, 3844, 4474
FblI	GTMKAC	1 cut(s)	4954
FriOI	GRGCYC	2 cut(s)	167, 184
FspI	TGCGCA	1 cut(s)	4287
GlaI	GCGC	4 cut(s)	2054, 3204, 4287, 5014
GsaI	CCCAGC	2 cut(s)	4314, 4384
GsuI	CTGGAG	3 cut(s)	151, 3425, 5346
HaeII	RGCGCY	2 cut(s)	2056, 5016
HapII	CCGG	8 cut(s)	128, 135, 178, 206, 1412, 3059, 3584, 5390
HhaI	GCGC	4 cut(s)	2055, 3205, 4288, 5015
Hin1I	GRCGYC	1 cut(s)	1243
Hin6I	GCGC	4 cut(s)	2053, 3203, 4286, 5013
HinP1I	GCGC	4 cut(s)	2053, 3203, 4286, 5013
HincII	GTYRAC	5 cut(s)	1147, 2093, 2181, 5002, 5107
HindII	GTYRAC	5 cut(s)	1147, 2093, 2181, 5002, 5107
HindIII	AAGCTT	6 cut(s)	377, 1007, 2891, 3918, 4043, 4433
HpaII	CCGG	8 cut(s)	128, 135, 178, 206, 1412, 3059, 3584, 5390
HphI	GGTGA	9 cut(s)	155, 598, 677, 1232, 1744, 1994, 2054, 3715, 5430
HpyCH4IV	ACGT	7 cut(s)	1243, 1702, 1978, 3050, 4590, 4810, 5567
HpySE526I	ACGT	7 cut(s)	1243, 1702, 1978, 3050, 4590, 4810, 5567
Hsp92I	GRCGYC	1 cut(s)	1243
HspAI	GCGC	4 cut(s)	2053, 3203, 4286, 5013
KflI	GGGWCCC	1 cut(s)	131
Ksp22I	TGATCA	4 cut(s)	1495, 3705, 3844, 4474
LguI	GCTCTTC	1 cut(s)	2755
Lsp1109I	GCAGC	7 cut(s)	730, 2471, 2497, 2623, 2752, 4893, 5071
MaeII	ACGT	7 cut(s)	1243, 1702, 1978, 3050, 4590, 4810, 5567
MaeIII	GTNAC	6 cut(s)	1059, 3304, 3611, 4507, 4910, 4987
MbiI	CCGCTC	3 cut(s)	141, 1367, 4442
MflI	RGATCY	5 cut(s)	2384, 2434, 3092, 3736, 5290
MhlI	GDGCHC	7 cut(s)	167, 175, 184, 722, 1474, 1978, 4533
MlsI	TGGCCA	3 cut(s)	3099, 3627, 4093
MluNI	TGGCCA	3 cut(s)	3099, 3627, 4093
MlyI	GAGTC	8 cut(s)	942, 1324, 1760, 2058, 2540, 3527, 3914, 4763
MmeI	TCCRAC	9 cut(s)	414, 438, 600, 910, 1647, 1788, 2562, 2690, 4886
Mox20I	TGGCCA	3 cut(s)	3099, 3627, 4093
Mph1103I	ATGCAT	1 cut(s)	1835
MroXI	GAANNNNTTC	1 cut(s)	1538
MscI	TGGCCA	3 cut(s)	3099, 3627, 4093
MslI	CAYNNNNRTG	6 cut(s)	2844, 3003, 3430, 4470, 4962, 5383
Msp20I	TGGCCA	3 cut(s)	3099, 3627, 4093
MspA1I	CMGCKG	3 cut(s)	1401, 3869, 5303
MspCI	CTTAAG	1 cut(s)	2393
MspI	CCGG	8 cut(s)	128, 135, 178, 206, 1412, 3059, 3584, 5390
Mva1269I	GAATGC	3 cut(s)	349, 4832, 5180
MvaI	CCWGG	8 cut(s)	169, 498, 939, 1230, 1929, 2283, 3661, 4685
NciI	CCSGG	7 cut(s)	129, 135, 136, 179, 207, 1413, 3060
NcoI	CCATGG	2 cut(s)	3001, 5532
NdeI	CATATG	3 cut(s)	1205, 4467, 5380
NheI	GCTAGC	1 cut(s)	3970
NmeAIII	GCCGAG	1 cut(s)	39
NmuCI	GTSAC	2 cut(s)	1059, 3611
NsbI	TGCGCA	1 cut(s)	4287
NsiI	ATGCAT	1 cut(s)	1835
NspI	RCATGY	5 cut(s)	1974, 2598, 2903, 3491, 5406
NspV	TTCGAA	2 cut(s)	1605, 2975
OliI	CACNNNNGTG	1 cut(s)	3003
PaeI	GCATGC	1 cut(s)	2903
PaeR7I	CTCGAG	1 cut(s)	2214
PagI	TCATGA	2 cut(s)	967, 5263
PaqCI	CACCTGC	2 cut(s)	3502, 5449
PceI	AGGCCT	1 cut(s)	4191
PciI	ACATGT	2 cut(s)	2594, 5402
PciSI	GCTCTTC	1 cut(s)	2755
PctI	GAATGC	3 cut(s)	349, 4832, 5180
PdmI	GAANNNNTTC	1 cut(s)	1538
PflMI	CCANNNNNTGG	2 cut(s)	2381, 3700
PfoI	TCCNGGA	1 cut(s)	1927
PleI	GAGTC	8 cut(s)	941, 1323, 1760, 2058, 2539, 3526, 3913, 4762
PmaCI	CACGTG	1 cut(s)	1979
PmlI	CACGTG	1 cut(s)	1979
PpsI	GAGTC	8 cut(s)	941, 1323, 1760, 2058, 2539, 3526, 3913, 4762
Ppu21I	YACGTR	1 cut(s)	1979
PpuMI	RGGWCCY	2 cut(s)	131, 4055
PscI	ACATGT	2 cut(s)	2594, 5402
PshBI	ATTAAT	1 cut(s)	1961
Psp124BI	GAGCTC	1 cut(s)	167
Psp5II	RGGWCCY	2 cut(s)	131, 4055
Psp6I	CCWGG	8 cut(s)	167, 496, 937, 1228, 1927, 2281, 3659, 4683
PspCI	CACGTG	1 cut(s)	1979
PspFI	CCCAGC	2 cut(s)	4310, 4380
PspGI	CCWGG	8 cut(s)	167, 496, 937, 1228, 1927, 2281, 3659, 4683
PspOMI	GGGCCC	1 cut(s)	180
PspPPI	RGGWCCY	2 cut(s)	131, 4055
PsrI	GAACNNNNNNTAC	2 cut(s)	4251, 4283
PstI	CTGCAG	3 cut(s)	757, 2442, 4066
PstNI	CAGNNNCTG	4 cut(s)	1184, 3641, 4000, 4946
PsuI	RGATCY	5 cut(s)	2384, 2434, 3092, 3736, 5290
PvuII	CAGCTG	1 cut(s)	3869
RsaI	GTAC	8 cut(s)	882, 1522, 1562, 1751, 3010, 3053, 4260, 4539
RsaNI	GTAC	8 cut(s)	881, 1521, 1561, 1750, 3009, 3052, 4259, 4538
RseI	CAYNNNNRTG	6 cut(s)	2844, 3003, 3430, 4470, 4962, 5383
SacI	GAGCTC	1 cut(s)	167
SapI	GCTCTTC	1 cut(s)	2755
ScaI	AGTACT	2 cut(s)	1562, 4539
SchI	GAGTC	8 cut(s)	942, 1324, 1760, 2058, 2540, 3527, 3914, 4763
SduI	GDGCHC	7 cut(s)	167, 175, 184, 722, 1474, 1978, 4533
SfcI	CTRYAG	5 cut(s)	753, 2438, 3171, 4062, 4992
Sfr274I	CTCGAG	1 cut(s)	2214
SfuI	TTCGAA	2 cut(s)	1605, 2975
SinI	GGWCC	5 cut(s)	131, 1253, 2835, 4055, 4076
SlaI	CTCGAG	1 cut(s)	2214
SmaI	CCCGGG	1 cut(s)	136
SmiMI	CAYNNNNRTG	6 cut(s)	2844, 3003, 3430, 4470, 4962, 5383
SmlI	CTYRAG	5 cut(s)	2214, 2393, 2454, 4271, 5068
SmoI	CTYRAG	5 cut(s)	2214, 2393, 2454, 4271, 5068
SphI	GCATGC	1 cut(s)	2903
SseBI	AGGCCT	1 cut(s)	4191
SspI	AATATT	4 cut(s)	1128, 2106, 3222, 4156
SstI	GAGCTC	1 cut(s)	167
StuI	AGGCCT	1 cut(s)	4191
StyI	CCWWGG	4 cut(s)	3001, 3834, 4079, 5532
TaiI	ACGT	7 cut(s)	1246, 1705, 1981, 3053, 4593, 4813, 5570
TaqII	GACCGA	1 cut(s)	3621
TatI	WGTACW	4 cut(s)	1520, 1560, 1749, 4537
TauI	GCSGC	3 cut(s)	214, 1367, 2281
TseFI	GTSAC	2 cut(s)	1059, 3611
TseI	GCWGC	7 cut(s)	743, 2459, 2510, 2636, 2765, 4881, 5084
Tsp45I	GTSAC	2 cut(s)	1059, 3611
TspGWI	ACGGA	4 cut(s)	2377, 2982, 3207, 4276
TspMI	CCCGGG	1 cut(s)	134
Van91I	CCANNNNNTGG	2 cut(s)	2381, 3700
Vha464I	CTTAAG	1 cut(s)	2393
VneI	GTGCAC	3 cut(s)	718, 1470, 1974
VpaK11BI	GGWCC	5 cut(s)	131, 1253, 2835, 4055, 4076
VspI	ATTAAT	1 cut(s)	1961
XagI	CCTNNNNNAGG	3 cut(s)	1902, 1932, 2803
XapI	RAATTY	9 cut(s)	1190, 2482, 2970, 2977, 3685, 5208, 5313, 5540, 5559
XceI	RCATGY	5 cut(s)	1974, 2598, 2903, 3491, 5406
XhoI	CTCGAG	1 cut(s)	2214
XmaI	CCCGGG	1 cut(s)	134
XmiI	GTMKAC	1 cut(s)	4954
XmnI	GAANNNNTTC	1 cut(s)	1538
ZraI	GACGTC	1 cut(s)	1244
ZrmI	AGTACT	2 cut(s)	1562, 4539
Zsp2I	ATGCAT	1 cut(s)	1835

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.