MD12G1012600.v1.1

WUSCHEL-related homeobox

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009573
Physical Location & Seq
Forward (+)
1324018 .. 1325972
1955 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1012600.v1.1.491

Sequence Viewer

Length: 903 bp
ATGGAAGATCATCATCAAGGCCAAGACCCTAACGATAGCAGTCCAAGCAACGGCTCGACCAAGAGAAGCCCTGAGCCGGTGAGGTCAAGATGGATACCGAAGCCACAGCAAATCCTAATTCTGGAGTCAATTTTCAACAGTGGAATGGTGAATCCTCCCAAAGAAGAAACTGTGAGAATAAGGAAACTGCTTGAGAAGTTTGGCTCCGTTGGGGATGCCAACGTTTTCTACTGGTTCCAAAACCGACGGTCGCGATCTCGCCGCCGACAACGGCAGTTGCAGGCCAGCCTTGAACAAAGAACCAATTATAATATAAACAATAATCAAATGGCTTCTCTTTCTCAACACCAAGTGGGTGGTGCAATTCAATATGGAGTAAGCTGCTGTGTTCCTACTGCTGCTCCTCCTTTGGCTTTCGGAGCTTCTCCTAATAATTTTCTTGTGGGCTCTTCATCTTCGTCTTGTGGTCAACATCTGATAGCAGATCATGATGTTCATCATAGTATTGATTGCGTTGGTGATCAGTTCTTTTCTGTTTCTGATCAAATGGGGTTTCCGGAAATCGAGCAGAGCTCCGGGGTAACGTCTGTGTTAGGAGGAGGCCCTTCTGATACCTCAAATTTGCACTTCCAATCTGGTCTCATCACAGTGTTTATTAATGGGATTCCAACAGAAGTTCCCAACGGGCCACTTGACATGAAAGCCGTGTTTGGACAAGATGTGCTATTGGTTCATTCCTCTGGACTCCCACTTCCAATCAATGAATTTGGTTTTTTGGCACACAGCTTGGAGTCTGGTGAAAGCTATTTCCTGGTACTCTCTCTCTCTCTCTCTCTCATGACTTATATTAATGAATTTACATTCTTCTGTATACCGCTAACCATTTTGTTCTTTGCAAATTAA

Protein Analysis

301

Amino Acids

32.96

Weight (kDa)

5.77

Isoelectric Point (pI)

60.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 28 - 88 4.1e-10 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009573)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03660 AT3G03660 AT3G03660 AT3G03660 AT5G17810 AT5G17810 AT5G17810
fragaria_vesca FvH4_1g29830
malus_domestica MD12G1012600.v1.1 MD14G1010400.v1.1
prunus_persica Prupe.7G016600_v2.0.a1
pyrus_communis pycom12g01010
rosa_chinensis RchiOBHm_Chr3g0495921
rosa_laevigata RLG00000022580
rosa_multiflora Rmu_sc0010892.1_g000017
rosa_roxburghii Rroxscaffold_6G00389910
rosa_rugosa Rorug03G0277600
rosa_samantha Rh3AG325000 Rh3BG361000 Rh3CG357300 Rh3DG360600
rosa_wichuraiana Rw3G028380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 309
AccI GTMKAC 1 cut(s) 871
AccII CGCG 1 cut(s) 253
AccIII TCCGGA 1 cut(s) 556
AciI CCGC 2 cut(s) 262, 875
AclI AACGTT 1 cut(s) 222
AcsI RAATTY 3 cut(s) 619, 764, 854
AdeI CACNNNGTG 1 cut(s) 352
AfaI GTAC 1 cut(s) 816
AfiI CCNNNNNNNGG 3 cut(s) 50, 76, 121
AgsI TTSAA 3 cut(s) 136, 293, 368
AjnI CCWGG 1 cut(s) 810
AluBI AGCT 5 cut(s) 381, 422, 573, 786, 804
AluI AGCT 5 cut(s) 381, 422, 573, 786, 804
Alw21I GWGCWC 1 cut(s) 575
Alw26I GTCTC 1 cut(s) 644
Aor13HI TCCGGA 1 cut(s) 556
AoxI GGCC 4 cut(s) 19, 282, 601, 686
ApeKI GCWGC 2 cut(s) 381, 398
ApoI RAATTY 3 cut(s) 619, 764, 854
AseI ATTAAT 2 cut(s) 657, 849
AspS9I GGNCC 2 cut(s) 602, 686
AsuC2I CCSGG 1 cut(s) 577
AsuHPI GGTGA 4 cut(s) 91, 160, 530, 809
BanII GRGCYC 2 cut(s) 449, 575
Bbv12I GWGCWC 1 cut(s) 575
BbvI GCAGC 2 cut(s) 368, 385
BccI CCATC 1 cut(s) 84
BceAI ACGGC 3 cut(s) 67, 287, 689
BciT130I CCWGG 1 cut(s) 812
BciVI GTATCC 1 cut(s) 87
BclI TGATCA 2 cut(s) 520, 541
BcnI CCSGG 1 cut(s) 577
BcoDI GTCTC 1 cut(s) 644
BfuI GTATCC 1 cut(s) 87
BisI GCNGC 3 cut(s) 262, 382, 399
BlsI GCNGC 3 cut(s) 263, 383, 400
Bme1390I CCNGG 2 cut(s) 577, 812
BmgT120I GGNCC 2 cut(s) 602, 686
BmiI GGNNCC 2 cut(s) 205, 236
BmrFI CCNGG 2 cut(s) 577, 812
BmsI GCATC 1 cut(s) 205
BplI GAGNNNNNCTC 2 cut(s) 557, 589
BpmI CTGGAG 1 cut(s) 143
Bpu10I CCTNAGC 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 1 cut(s) 577
BsaBI GATNNNNATC 2 cut(s) 12, 495
BsaI GGTCTC 1 cut(s) 644
BsaJI CCNNGG 1 cut(s) 576
BsaWI WCCGGW 1 cut(s) 556
BsaXI ACNNNNNCTCC 2 cut(s) 385, 415
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 76, 121
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 236
Bse8I GATNNNNATC 2 cut(s) 12, 495
BseAI TCCGGA 1 cut(s) 556
BseBI CCWGG 1 cut(s) 812
BseDI CCNNGG 1 cut(s) 576
BseGI GGATG 1 cut(s) 220
BseJI GATNNNNATC 2 cut(s) 12, 495
BseLI CCNNNNNNNGG 3 cut(s) 50, 76, 121
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 1 cut(s) 236
BseRI GAGGAG 2 cut(s) 393, 612
BseXI GCAGC 2 cut(s) 368, 385
Bsh1236I CGCG 1 cut(s) 253
Bsh1285I CGRYCG 1 cut(s) 251
BshFI GGCC 4 cut(s) 21, 284, 603, 688
BsiEI CGRYCG 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 575
BsiSI CCGG 3 cut(s) 77, 557, 576
BslI CCNNNNNNNGG 3 cut(s) 50, 76, 121
BsmAI GTCTC 1 cut(s) 644
BsnI GGCC 4 cut(s) 21, 284, 603, 688
Bso31I GGTCTC 1 cut(s) 644
Bsp1286I GDGCHC 2 cut(s) 449, 575
Bsp13I TCCGGA 1 cut(s) 556
Bsp143I GATC 5 cut(s) 7, 254, 484, 520, 541
Bsp68I TCGCGA 1 cut(s) 253
BspACI CCGC 2 cut(s) 262, 875
BspANI GGCC 4 cut(s) 21, 284, 603, 688
BspCNI CTCAG 1 cut(s) 64
BspEI TCCGGA 1 cut(s) 556
BspFNI CGCG 1 cut(s) 253
BspHI TCATGA 2 cut(s) 487, 837
BspLI GGNNCC 2 cut(s) 205, 236
BspQI GCTCTTC 1 cut(s) 454
BspTNI GGTCTC 1 cut(s) 644
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 1 cut(s) 236
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 1 cut(s) 576
BssMI GATC 5 cut(s) 7, 254, 484, 520, 541
BssNAI GTATAC 1 cut(s) 872
Bst1107I GTATAC 1 cut(s) 872
Bst2UI CCWGG 1 cut(s) 812
Bst4CI ACNGT 4 cut(s) 140, 172, 249, 649
Bst6I CTCTTC 1 cut(s) 454
BstC8I GCNNGC 2 cut(s) 282, 286
BstDEI CTNAG 1 cut(s) 72
BstF5I GGATG 1 cut(s) 220
BstFNI CGCG 1 cut(s) 253
BstKTI GATC 5 cut(s) 10, 257, 487, 523, 544
BstMAI GTCTC 1 cut(s) 644
BstMBI GATC 5 cut(s) 7, 254, 484, 520, 541
BstMCI CGRYCG 1 cut(s) 251
BstMWI GCNNNNNNNGC 2 cut(s) 45, 419
BstNI CCWGG 1 cut(s) 812
BstSCI CCNGG 2 cut(s) 575, 810
BstUI CGCG 1 cut(s) 253
BstV1I GCAGC 2 cut(s) 368, 385
BstXI CCANNNNNNTGG 1 cut(s) 356
BstZ17I GTATAC 1 cut(s) 872
BsuI GTATCC 1 cut(s) 87
BsuRI GGCC 4 cut(s) 21, 284, 603, 688
BtsCI GGATG 1 cut(s) 220
BtsIMutI CAGTG 2 cut(s) 145, 654
BtuMI TCGCGA 1 cut(s) 253
Cac8I GCNNGC 2 cut(s) 282, 286
CciI TCATGA 2 cut(s) 487, 837
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 2 cut(s) 602, 686
Csp6I GTAC 1 cut(s) 815
CviAII CATG 3 cut(s) 488, 697, 838
CviQI GTAC 1 cut(s) 815
DdeI CTNAG 1 cut(s) 72
DpnI GATC 5 cut(s) 9, 256, 486, 522, 543
DpnII GATC 5 cut(s) 7, 254, 484, 520, 541
DraIII CACNNNGTG 1 cut(s) 352
Eam1104I CTCTTC 1 cut(s) 454
EarI CTCTTC 1 cut(s) 454
Ecl136II GAGCTC 1 cut(s) 573
Eco24I GRGCYC 2 cut(s) 449, 575
Eco31I GGTCTC 1 cut(s) 644
Eco53kI GAGCTC 1 cut(s) 573
EcoICRI GAGCTC 1 cut(s) 573
EcoO109I RGGNCCY 1 cut(s) 602
EcoRII CCWGG 1 cut(s) 810
EcoT38I GRGCYC 2 cut(s) 449, 575
FaeI CATG 3 cut(s) 491, 700, 841
FaiI YATR 9 cut(s) 309, 314, 372, 489, 501, 698, 839, 846, 872
FatI CATG 3 cut(s) 487, 696, 837
FbaI TGATCA 2 cut(s) 520, 541
FblI GTMKAC 1 cut(s) 871
Fnu4HI GCNGC 3 cut(s) 262, 382, 399
FokI GGATG 1 cut(s) 227
FriOI GRGCYC 2 cut(s) 449, 575
Fsp4HI GCNGC 3 cut(s) 262, 382, 399
GluI GCNGC 3 cut(s) 262, 382, 399
GsuI CTGGAG 1 cut(s) 143
HaeIII GGCC 4 cut(s) 21, 284, 603, 688
HapII CCGG 3 cut(s) 77, 557, 576
Hin1II CATG 3 cut(s) 491, 700, 841
HincII GTYRAC 1 cut(s) 470
HindII GTYRAC 1 cut(s) 470
HinfI GANTC 5 cut(s) 125, 151, 664, 744, 791
HpaII CCGG 3 cut(s) 77, 557, 576
HphI GGTGA 4 cut(s) 91, 160, 530, 809
Hpy166II GTNNAC 2 cut(s) 470, 872
Hpy188I TCNGA 4 cut(s) 419, 477, 541, 610
Hpy188III TCNNGA 7 cut(s) 87, 122, 252, 488, 557, 741, 838
Hpy8I GTNNAC 2 cut(s) 470, 872
Hpy99I CGWCG 1 cut(s) 249
HpyAV CCTTC 1 cut(s) 615
HpyCH4III ACNGT 4 cut(s) 140, 172, 249, 649
HpyCH4IV ACGT 2 cut(s) 222, 584
HpyCH4V TGCA 4 cut(s) 280, 362, 625, 896
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 419
HpyF3I CTNAG 1 cut(s) 72
HpySE526I ACGT 2 cut(s) 222, 584
Hsp92II CATG 3 cut(s) 491, 700, 841
Kpn2I TCCGGA 1 cut(s) 556
Ksp22I TGATCA 2 cut(s) 520, 541
Kzo9I GATC 5 cut(s) 7, 254, 484, 520, 541
LguI GCTCTTC 1 cut(s) 454
LmnI GCTCC 4 cut(s) 209, 406, 419, 578
Lsp1109I GCAGC 2 cut(s) 368, 385
LweI GCATC 1 cut(s) 205
MaeII ACGT 2 cut(s) 222, 584
MaeIII GTNAC 1 cut(s) 580
MalI GATC 5 cut(s) 9, 256, 486, 522, 543
MboI GATC 5 cut(s) 7, 254, 484, 520, 541
MboII GAAGA 5 cut(s) 17, 176, 441, 447, 856
MhlI GDGCHC 2 cut(s) 449, 575
MluCI AATT 9 cut(s) 117, 129, 304, 363, 433, 619, 764, 854, 898
MlyI GAGTC 3 cut(s) 134, 738, 800
MmeI TCCRAC 1 cut(s) 692
MnlI CCTC 7 cut(s) 75, 165, 414, 590, 593, 625, 748
MroI TCCGGA 1 cut(s) 556
MseI TTAA 3 cut(s) 657, 849, 901
MslI CAYNNNNRTG 1 cut(s) 647
MspI CCGG 3 cut(s) 77, 557, 576
MspR9I CCNGG 2 cut(s) 577, 812
MvaI CCWGG 1 cut(s) 812
MvnI CGCG 1 cut(s) 253
MwoI GCNNNNNNNGC 2 cut(s) 45, 419
NciI CCSGG 1 cut(s) 577
NdeII GATC 5 cut(s) 7, 254, 484, 520, 541
NlaIII CATG 3 cut(s) 491, 700, 841
NlaIV GGNNCC 2 cut(s) 205, 236
NruI TCGCGA 1 cut(s) 253
PagI TCATGA 2 cut(s) 487, 837
PciSI GCTCTTC 1 cut(s) 454
PfeI GAWTC 2 cut(s) 151, 664
PkrI GCNGC 3 cut(s) 263, 383, 400
PleI GAGTC 3 cut(s) 133, 738, 799
PpsI GAGTC 3 cut(s) 133, 738, 799
PshBI ATTAAT 2 cut(s) 657, 849
PsiI TTATAA 1 cut(s) 309
Psp124BI GAGCTC 1 cut(s) 575
Psp1406I AACGTT 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 810
PspGI CCWGG 1 cut(s) 810
PspN4I GGNNCC 2 cut(s) 205, 236
PspPI GGNCC 2 cut(s) 602, 686
RruI TCGCGA 1 cut(s) 253
RsaI GTAC 1 cut(s) 816
RsaNI GTAC 1 cut(s) 815
RseI CAYNNNNRTG 1 cut(s) 647
SacI GAGCTC 1 cut(s) 575
SapI GCTCTTC 1 cut(s) 454
SaqAI TTAA 3 cut(s) 657, 849, 901
SatI GCNGC 3 cut(s) 262, 382, 399
Sau3AI GATC 5 cut(s) 7, 254, 484, 520, 541
Sau96I GGNCC 2 cut(s) 602, 686
SchI GAGTC 3 cut(s) 134, 738, 800
ScrFI CCNGG 2 cut(s) 577, 812
SduI GDGCHC 2 cut(s) 449, 575
SetI ASST 9 cut(s) 86, 225, 383, 424, 575, 587, 617, 788, 806
SfaNI GCATC 1 cut(s) 205
SmiMI CAYNNNNRTG 1 cut(s) 647
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
Sse9I AATT 9 cut(s) 117, 129, 304, 363, 433, 619, 764, 854, 898
SsiI CCGC 2 cut(s) 262, 875
SstI GAGCTC 1 cut(s) 575
StyD4I CCNGG 2 cut(s) 575, 810
TaaI ACNGT 4 cut(s) 140, 172, 249, 649
TaiI ACGT 2 cut(s) 225, 587
TaqI TCGA 2 cut(s) 56, 564
TasI AATT 9 cut(s) 117, 129, 304, 363, 433, 619, 764, 854, 898
TauI GCSGC 1 cut(s) 264
TfiI GAWTC 2 cut(s) 151, 664
Tru1I TTAA 3 cut(s) 657, 849, 901
Tru9I TTAA 3 cut(s) 657, 849, 901
TscAI CASTG 2 cut(s) 145, 654
TseI GCWGC 2 cut(s) 381, 398
TspDTI ATGAA 6 cut(s) 441, 485, 713, 722, 777, 867
TspGWI ACGGA 1 cut(s) 196
TspRI CASTG 2 cut(s) 145, 654
VspI ATTAAT 2 cut(s) 657, 849
XapI RAATTY 3 cut(s) 619, 764, 854
XmiI GTMKAC 1 cut(s) 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.