Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0010490

Physical Location & Seq

Genomic Coordinates Forward (+)

1634423 .. 1639379

Gene Structure 4957 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1016300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2505 bp

ATGGCAAGGTGCCTCTTCTTTCATCGGAAATCCATATTGAATGCCCTCAACAAACCCCAAAATCCAATCTCAGTCCCTCTCTCGTTCAATCTCTGCACAGACTCATTTGCCAACCAAACTCATCAACCCCAGATTGAAGACCCAACAGAGAACCACAACCCCACCAACCTCCAGGAGCAACAACACCATGAGCAAATCCGAAAGCTCCGAGTCCTCCTTCAGCAGGGTCGTTCTGAAACCGCACGGAGGCTCATCAAGTCTCTCGTTCTCCCCAGTTCACCCTTCTCTTCCCCCACTGAGCTCTACACCTTGTTTTCTCTCTCTGCACCCTCCATGAAACCCGCATTCTGTGACATGCTTTTAGTGGCTTGTTCAGAGTCTAAGATGCCGAACGAAGCAGTAGAATTGTACAGTTTGATGAGGAAAAGTGGTACACGCCCTTGCTTAGCTTCTCTCAATGTGATGCTTGAATCTTTGGTGACTTCGAAACAGTTTGGTAAGACCCTTGAATTGTTTTCGGAGATTTTTGAGTCCGGTCAGGGCATTCGACCTGATAAGTTCACGTATGGGAAGGCAATACAAGCTTCTGTAAAGTTAGGGGACCTGGAAAGGGCTGATCAGCTAGTGAATTCAATGAAGATGAAGCGGATGAGTCCAGGTGTGTTTGTGTATAATGTGTTGCTCAGCGGGATGTGTAAAGAGAAGAAAATGAGGGATGCACAGAAGGTGTTTGATGAGATGGTTGAGGGAAAGGTGGCCCCAAATTTGGTTACATACAATACGATGATTGATGGGTATTCTAAGGTTGGTGAGTTGGAGAAGGCCTTTGAATTGAGAGAGAGGATGAAGGATGAGAATGTGGAAGCTAATATGGTCACATATAATACAATGCTTAGTGGACTTTGTCGGGTGAAGAGAATGGAGGACGCGAAAAGGATTTTAGAAGAGATGGAAGCTCACGGCTTTGCGCCTGATGGTTTTACTTATAGCATACTCTTTGATGGGCATTTCAGGTGTGGGGATGGTGAGGGGTCACTGGCTTTATTTGAAGAAGCAAACAGGAAAGGTGTGAGGATTAATAGTTATACTTGTAGCATTTTGTTGAATGGCTTGTGCAAGGAAGGAAACATTGAGAAGGCAGAGGACGTTTTGAGGAAACTAATGGAAACTGGTTTTGTTCCAGATGTAGTAGTATATAATACTATCGTCAGTGGTTATTGTCGAATTGGTGAGATAGACAAAGCCATCTTGGCCATTGAACAAATGGAAGTTCGCGGGTTGAAGCCGAATTGCATCACTTTCAATTCTTTGATTGACAAGGTCTGTGAGACAAAGAATATGGATATGGCTGAGGAATGGGTGAAGAAGATGGCAAAGAAGGGAGTTATTCCAAATTTGGAGACGTATAACATCCTTATTAATGGCTACGGGCAGATGTGCGTATTTGATAAGTGTTTCCAGATTCTTGAAGTGATGGAAAATAAGGGGATTAAGCCAAATATTGTGAGCTATGGTTCTCTTGTAAATGGTTTATGCAAAGACGGGAGGCTTCTTGAAGCTGAGATAGTACTGAGGGACATGGTAAGCAGAGGGGTTTTGCCAAATGCACAAATATATAATATGCTCATTGGTGGTACATCTACAGTGGGGAATCTGAAAGATGCTTTCAGATTTTTCGATGAGATGGCGAAAACTGGGATAAATCCAACACTTGTAACCTACAATGCACTCATTCATGGACTCTGCAAGAAAGGACGGGTGATGGAAGCTGAAGACTATGTCTCTCAAATTACAAGTAGCGGTTATAGTGCAGATGTGATTACATACAACTCCCTAATTGCAGGTTATTCTGATGCAGGAAACACCCAGAAATGTCTGGAATTATATGAAACTATCAAGAGTCTGGGCATGAAACCTACTTTGTATACATATCATCCTCTAATTAGTCGATGTAGCAGGGAAGATATGGCATTGGCAGAGAAGCTGTATAGTGAAATGCTACAGATGGGCTTGGTTCCTGATCGGATTGTATATAATGCACTCATCCATGGTTACGCAGAGCATGGAGACACTCAAAAGGCGCTTGCTTTGCACTCTGAGATGGTAAACCAGAAGATTTATGTTGACAGGATGACATATAATAGCTTGATTCTGGGGCACTTCAAGCAAGGAAAAATTTCAGAGGTAAAGGACTTAGTAAATGATATGAAGGCTCAAGGACTGGCTCCTAAAGCTGATACATATAACTTACTTGTTAAGGGACACTGCGAACTGAAGGACTTCAGTGGTGCATATTTTTGGTATAGGGAACTGTTTAAGAATGGTTTTCTTCTAAACGCCTTGACGTGCCTTGAACTTACATCTGGCCTTCAAAAGGAGGGGAGGTTGCGAGAGGCCGAGATTGTCTGCTCAGAAATGAGCGTCAAAGGATTGAATGACTGCAGTTCTAATGAGGATGTGTTTTCTGTTGCCAATGTAAAGGGAACAGATGTTTGCCATTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0046872 GO:0046914 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

835

Amino Acids

93.53

Weight (kDa)

6.37

Isoelectric Point (pI)

31.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	180 - 229	4.2e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	217 - 248	1.9e-08	PPR repeat
PPR_2	PF13041	219 - 268	3.4e-17	PPR repeat family
PPR_3	PF13812	222 - 266	2.1e-08	Pentatricopeptide repeat domain
PPR	PF01535	223 - 249	3.5e-06	PPR repeat
PPR_long	PF17177	236 - 342	3.1e-09	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	253 - 283	3.1e-10	PPR repeat
PPR_2	PF13041	254 - 303	1.1e-18	PPR repeat family
PPR	PF01535	257 - 287	2.2e-09	PPR repeat
PPR_3	PF13812	280 - 332	3.7e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	286 - 318	5.8e-10	PPR repeat
PPR_2	PF13041	290 - 337	2.5e-16	PPR repeat family
PPR	PF01535	292 - 322	6e-08	PPR repeat
PPR_2	PF13041	332 - 373	4.6e-08	PPR repeat family
PPR_3	PF13812	348 - 405	2.2e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	355 - 386	3e-06	PPR repeat
TPR_24	PF23276	364 - 475	3.4e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	391 - 423	2.3e-10	PPR repeat
PPR_2	PF13041	394 - 442	2.2e-14	PPR repeat family
PPR_long	PF17177	419 - 509	5.5e-10	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	420 - 479	8.3e-13	Pentatricopeptide repeat domain
PPR_2	PF13041	432 - 476	1.3e-11	PPR repeat family
PPR_2	PF13041	468 - 513	1.8e-12	PPR repeat family
PPR_1	PF12854	495 - 528	2.2e-10	PPR repeat
PPR_2	PF13041	499 - 545	8.3e-10	PPR repeat family
PPR	PF01535	502 - 532	3.3e-06	PPR repeat
PPR_3	PF13812	523 - 579	2.5e-13	Pentatricopeptide repeat domain
PPR_long	PF17177	524 - 647	3.8e-08	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	524 - 631	1.7e-07	Fungal tetratrico peptide repeats
PPR_1	PF12854	530 - 562	3.1e-06	PPR repeat
PPR_2	PF13041	534 - 583	8.7e-15	PPR repeat family
PPR_1	PF12854	566 - 592	9.2e-11	PPR repeat
PPR_2	PF13041	569 - 613	1.3e-10	PPR repeat family
PPR	PF01535	572 - 601	4.8e-06	PPR repeat
PPR_3	PF13812	600 - 651	3.2e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	605 - 652	2.5e-10	PPR repeat family
TPR_24	PF23276	655 - 770	8e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	669 - 702	6.1e-07	PPR repeat
PPR_2	PF13041	673 - 717	2.3e-10	PPR repeat family
PPR	PF01535	677 - 703	5.8e-06	PPR repeat
PPR_3	PF13812	698 - 753	1.8e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	710 - 756	2.7e-11	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010490)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12100
fragaria_vesca	FvH4_6g17750 FvH4_6g17750 FvH4_6g17750 FvH4_6g17750
malus_domestica	MD12G1016300.v1.1 MD14G1014400.v1.1
prunus_persica	Prupe.7G119000_v2.0.a1
pyrus_communis	pycom12g01340 pycom14g01210
rosa_chinensis	RchiOBHm_Chr3g0471971
rosa_laevigata	RLG00000024115
rosa_multiflora	Rmu_sc0000090.1_g000022
rosa_roxburghii	Rroxscaffold_6G00409400
rosa_rugosa	Rorug03G0121400
rosa_samantha	Rh3AG172800 Rh3BG198300 Rh3CG189400 Rh3CG196800 Rh3DG176100
rosa_wichuraiana	Rw3G016780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1832
AccB1I	GGYRCC	1 cut(s)	9
AccI	GTMKAC	1 cut(s)	1925
AccII	CGCG	2 cut(s)	929, 1275
AciI	CCGC	6 cut(s)	240, 342, 646, 687, 1275, 1800
AcoI	YGGCCR	1 cut(s)	1251
AcsI	RAATTY	4 cut(s)	628, 763, 1393, 2175
AcuI	CTGAAG	4 cut(s)	203, 1791, 2266, 2294
AfaI	GTAC	4 cut(s)	410, 433, 1569, 1636
AfiI	CCNNNNNNNGG	5 cut(s)	223, 246, 610, 766, 2374
AjiI	CACGTC	1 cut(s)	2346
AjnI	CCWGG	3 cut(s)	171, 603, 655
Alw21I	GWGCWC	1 cut(s)	303
Alw26I	GTCTC	5 cut(s)	264, 1322, 1394, 1786, 2061
AoxI	GGCC	5 cut(s)	756, 822, 1251, 2365, 2394
ApoI	RAATTY	4 cut(s)	628, 763, 1393, 2175
AseI	ATTAAT	2 cut(s)	1077, 1419
Asp700I	GAANNNNTTC	2 cut(s)	2176, 2279
AspLEI	GCGC	2 cut(s)	970, 2083
AspS9I	GGNCC	2 cut(s)	601, 757
AsuHPI	GGTGA	8 cut(s)	270, 490, 821, 922, 1037, 1241, 1372, 1771
AsuII	TTCGAA	1 cut(s)	485
AvaII	GGWCC	1 cut(s)	601
BaeGI	GKGCMC	1 cut(s)	2160
BalI	TGGCCA	1 cut(s)	1253
BanI	GGYRCC	1 cut(s)	9
BanII	GRGCYC	1 cut(s)	303
BbsI	GAAGAC	2 cut(s)	144, 1779
Bbv12I	GWGCWC	1 cut(s)	303
BbvCI	CCTCAGC	1 cut(s)	1350
BceAI	ACGGC	1 cut(s)	976
BciT130I	CCWGG	3 cut(s)	173, 605, 657
BclI	TGATCA	1 cut(s)	616
BcoDI	GTCTC	5 cut(s)	264, 1322, 1394, 1786, 2061
BfaI	CTAG	1 cut(s)	623
BfmI	CTRYAG	3 cut(s)	1641, 2000, 2440
BfoI	RGCGCY	1 cut(s)	2084
BfuAI	ACCTGC	1 cut(s)	1832
BglI	GCCNNNNNGGC	1 cut(s)	1250
BlpI	GCTNAGC	2 cut(s)	445, 683
BmcAI	AGTACT	1 cut(s)	1569
Bme1390I	CCNGG	3 cut(s)	173, 605, 657
Bme18I	GGWCC	1 cut(s)	601
BmgBI	CACGTC	1 cut(s)	2346
BmgT120I	GGNCC	2 cut(s)	601, 757
BmiI	GGNNCC	5 cut(s)	11, 602, 759, 2016, 2226
BmrFI	CCNGG	3 cut(s)	173, 605, 657
BmrI	ACTGGG	2 cut(s)	267, 1704
BmsI	GCATC	6 cut(s)	375, 453, 706, 1302, 1651, 1843
BmuI	ACTGGG	2 cut(s)	267, 1704
BpiI	GAAGAC	2 cut(s)	144, 1779
BpmI	CTGGAG	1 cut(s)	155
Bpu10I	CCTNAGC	1 cut(s)	1350
Bpu1102I	GCTNAGC	2 cut(s)	445, 683
Bpu14I	TTCGAA	1 cut(s)	485
BpuEI	CTTGAG	1 cut(s)	2199
BsaAI	YACGTR	1 cut(s)	564
BsaJI	CCNNGG	1 cut(s)	2047
BsaWI	WCCGGW	1 cut(s)	533
BsaXI	ACNNNNNCTCC	2 cut(s)	2369, 2399
Bsc4I	CCNNNNNNNGG	5 cut(s)	223, 246, 610, 766, 2374
Bse1I	ACTGG	5 cut(s)	273, 1041, 1174, 1699, 2226
BseBI	CCWGG	3 cut(s)	173, 605, 657
BseDI	CCNNGG	1 cut(s)	2047
BseLI	CCNNNNNNNGG	5 cut(s)	223, 246, 610, 766, 2374
BseMII	CTCAG	8 cut(s)	84, 288, 697, 1341, 1551, 1562, 2088, 2424
BseNI	ACTGG	5 cut(s)	273, 1041, 1174, 1699, 2226
BseSI	GKGCMC	1 cut(s)	2160
BsgI	GTGCAG	3 cut(s)	79, 309, 1830
Bsh1236I	CGCG	2 cut(s)	929, 1275
BshFI	GGCC	5 cut(s)	758, 824, 1253, 2367, 2396
BshNI	GGYRCC	1 cut(s)	9
BsiHKAI	GWGCWC	1 cut(s)	303
BsiSI	CCGG	1 cut(s)	534
BslFI	GGGAC	4 cut(s)	59, 614, 1589, 2274
BslI	CCNNNNNNNGG	5 cut(s)	223, 246, 610, 766, 2374
BsmAI	GTCTC	5 cut(s)	264, 1322, 1394, 1786, 2061
BsmBI	CGTCTC	1 cut(s)	1394
BsmFI	GGGAC	4 cut(s)	59, 614, 1589, 2274
BsmI	GAATGC	3 cut(s)	46, 344, 543
BsnI	GGCC	5 cut(s)	758, 824, 1253, 2367, 2396
Bsp119I	TTCGAA	1 cut(s)	485
Bsp1286I	GDGCHC	2 cut(s)	303, 2160
Bsp1407I	TGTACA	1 cut(s)	408
Bsp143I	GATC	2 cut(s)	616, 2020
Bsp1720I	GCTNAGC	2 cut(s)	445, 683
Bsp19I	CCATGG	1 cut(s)	2047
BspACI	CCGC	6 cut(s)	240, 342, 646, 687, 1275, 1800
BspANI	GGCC	5 cut(s)	758, 824, 1253, 2367, 2396
BspCNI	CTCAG	8 cut(s)	83, 289, 696, 1342, 1552, 1563, 2089, 2423
BspFNI	CGCG	2 cut(s)	929, 1275
BspLI	GGNNCC	5 cut(s)	11, 602, 759, 2016, 2226
BspMAI	CTGCAG	1 cut(s)	2444
BspMI	ACCTGC	1 cut(s)	1832
BspT104I	TTCGAA	1 cut(s)	485
BspT107I	GGYRCC	1 cut(s)	9
BsrGI	TGTACA	1 cut(s)	408
BsrI	ACTGG	5 cut(s)	273, 1041, 1174, 1699, 2226
BssECI	CCNNGG	1 cut(s)	2047
BssMI	GATC	2 cut(s)	616, 2020
BssNAI	GTATAC	1 cut(s)	1926
BssT1I	CCWWGG	1 cut(s)	2047
Bst1107I	GTATAC	1 cut(s)	1926
Bst2UI	CCWGG	3 cut(s)	173, 605, 657
Bst4CI	ACNGT	4 cut(s)	413, 492, 1645, 2313
Bst6I	CTCTTC	4 cut(s)	20, 292, 908, 939
BstAUI	TGTACA	1 cut(s)	408
BstBAI	YACGTR	1 cut(s)	564
BstBI	TTCGAA	1 cut(s)	485
BstC8I	GCNNGC	1 cut(s)	2085
BstDSI	CCRYGG	1 cut(s)	2047
BstENI	CCTNNNNNAGG	2 cut(s)	221, 2372
BstFNI	CGCG	2 cut(s)	929, 1275
BstH2I	RGCGCY	1 cut(s)	2084
BstHHI	GCGC	2 cut(s)	970, 2083
BstKTI	GATC	2 cut(s)	619, 2023
BstMAI	GTCTC	5 cut(s)	264, 1322, 1394, 1786, 2061
BstMBI	GATC	2 cut(s)	616, 2020
BstMWI	GCNNNNNNNGC	5 cut(s)	581, 1250, 2089, 2164, 2231
BstNI	CCWGG	3 cut(s)	173, 605, 657
BstNSI	RCATGY	1 cut(s)	358
BstSCI	CCNGG	3 cut(s)	171, 603, 655
BstSFI	CTRYAG	3 cut(s)	1641, 2000, 2440
BstSLI	GKGCMC	1 cut(s)	2160
BstUI	CGCG	2 cut(s)	929, 1275
BstV2I	GAAGAC	2 cut(s)	144, 1779
BstZ17I	GTATAC	1 cut(s)	1926
BsuRI	GGCC	5 cut(s)	758, 824, 1253, 2367, 2396
BtgI	CCRYGG	1 cut(s)	2047
BtrI	CACGTC	1 cut(s)	2346
BtsI	GCAGTG	1 cut(s)	2263
BtsIMutI	CAGTG	6 cut(s)	294, 1034, 1216, 1650, 2263, 2290
BveI	ACCTGC	1 cut(s)	1832
Cac8I	GCNNGC	1 cut(s)	2085
CfoI	GCGC	2 cut(s)	970, 2083
Cfr13I	GGNCC	2 cut(s)	601, 757
CseI	GACGC	2 cut(s)	935, 2410
Csp6I	GTAC	4 cut(s)	409, 432, 1568, 1635
CviAII	CATG	8 cut(s)	188, 334, 355, 1579, 1736, 1909, 2048, 2063
CviQI	GTAC	4 cut(s)	409, 432, 1568, 1635
DpnI	GATC	2 cut(s)	618, 2022
DpnII	GATC	2 cut(s)	616, 2020
EaeI	YGGCCR	1 cut(s)	1251
Eam1104I	CTCTTC	4 cut(s)	20, 292, 908, 939
EarI	CTCTTC	4 cut(s)	20, 292, 908, 939
Ecl136II	GAGCTC	1 cut(s)	301
Eco130I	CCWWGG	1 cut(s)	2047
Eco147I	AGGCCT	1 cut(s)	824
Eco24I	GRGCYC	1 cut(s)	303
Eco47I	GGWCC	1 cut(s)	601
Eco53kI	GAGCTC	1 cut(s)	301
Eco57I	CTGAAG	4 cut(s)	203, 1791, 2266, 2294
EcoICRI	GAGCTC	1 cut(s)	301
EcoNI	CCTNNNNNAGG	2 cut(s)	221, 2372
EcoO109I	RGGNCCY	1 cut(s)	601
EcoRI	GAATTC	1 cut(s)	628
EcoRII	CCWGG	3 cut(s)	171, 603, 655
EcoT14I	CCWWGG	1 cut(s)	2047
EcoT38I	GRGCYC	1 cut(s)	303
ErhI	CCWWGG	1 cut(s)	2047
Esp3I	CGTCTC	1 cut(s)	1394
FaeI	CATG	8 cut(s)	191, 337, 358, 1582, 1739, 1912, 2051, 2066
FaqI	GGGAC	4 cut(s)	59, 614, 1589, 2274
FatI	CATG	8 cut(s)	187, 333, 354, 1578, 1735, 1908, 2047, 2062
FauI	CCCGC	3 cut(s)	349, 680, 1268
FbaI	TGATCA	1 cut(s)	616
FblI	GTMKAC	1 cut(s)	1925
FriOI	GRGCYC	1 cut(s)	303
FspBI	CTAG	1 cut(s)	623
GlaI	GCGC	2 cut(s)	969, 2082
GsuI	CTGGAG	1 cut(s)	155
HaeII	RGCGCY	1 cut(s)	2084
HaeIII	GGCC	5 cut(s)	758, 824, 1253, 2367, 2396
HapII	CCGG	1 cut(s)	534
HgaI	GACGC	2 cut(s)	935, 2410
HhaI	GCGC	2 cut(s)	970, 2083
Hin1II	CATG	8 cut(s)	191, 337, 358, 1582, 1739, 1912, 2051, 2066
Hin6I	GCGC	2 cut(s)	968, 2081
HinP1I	GCGC	2 cut(s)	968, 2081
HincII	GTYRAC	1 cut(s)	2125
HindII	GTYRAC	1 cut(s)	2125
HindIII	AAGCTT	1 cut(s)	582
HpaII	CCGG	1 cut(s)	534
HphI	GGTGA	8 cut(s)	270, 490, 821, 922, 1037, 1241, 1372, 1771
Hpy166II	GTNNAC	7 cut(s)	278, 434, 561, 899, 1926, 2107, 2125
Hpy188III	TCNNGA	8 cut(s)	1181, 1459, 1466, 1553, 1877, 1897, 2018, 2502
Hpy8I	GTNNAC	7 cut(s)	278, 434, 561, 899, 1926, 2107, 2125
HpyCH4III	ACNGT	4 cut(s)	413, 492, 1645, 2313
HpyCH4IV	ACGT	4 cut(s)	563, 1146, 1403, 2345
HpyF10VI	GCNNNNNNNGC	5 cut(s)	581, 1250, 2089, 2164, 2231
HpySE526I	ACGT	4 cut(s)	563, 1146, 1403, 2345
Hsp92II	CATG	8 cut(s)	191, 337, 358, 1582, 1739, 1912, 2051, 2066
HspAI	GCGC	2 cut(s)	968, 2081
Ksp22I	TGATCA	1 cut(s)	616
Kzo9I	GATC	2 cut(s)	616, 2020
LmnI	GCTCC	3 cut(s)	175, 210, 2230
LweI	GCATC	6 cut(s)	375, 453, 706, 1302, 1651, 1843
MaeI	CTAG	1 cut(s)	623
MaeII	ACGT	4 cut(s)	563, 1146, 1403, 2345
MaeIII	GTNAC	7 cut(s)	350, 478, 769, 874, 1032, 1714, 2051
MalI	GATC	2 cut(s)	618, 2022
MboI	GATC	2 cut(s)	616, 2020
MhlI	GDGCHC	2 cut(s)	303, 2160
MlsI	TGGCCA	1 cut(s)	1253
MluNI	TGGCCA	1 cut(s)	1253
MlyI	GAGTC	7 cut(s)	95, 219, 386, 539, 661, 1734, 1909
MmeI	TCCRAC	2 cut(s)	795, 1730
Mox20I	TGGCCA	1 cut(s)	1253
MroXI	GAANNNNTTC	2 cut(s)	2176, 2279
MscI	TGGCCA	1 cut(s)	1253
MseI	TTAA	5 cut(s)	1077, 1419, 1491, 2256, 2316
Msp20I	TGGCCA	1 cut(s)	1253
MspA1I	CMGCKG	1 cut(s)	687
MspI	CCGG	1 cut(s)	534
MspR9I	CCNGG	3 cut(s)	173, 605, 657
Mva1269I	GAATGC	3 cut(s)	46, 344, 543
MvaI	CCWGG	3 cut(s)	173, 605, 657
MvnI	CGCG	2 cut(s)	929, 1275
MwoI	GCNNNNNNNGC	5 cut(s)	581, 1250, 2089, 2164, 2231
NcoI	CCATGG	1 cut(s)	2047
NdeII	GATC	2 cut(s)	616, 2020
NlaIII	CATG	8 cut(s)	191, 337, 358, 1582, 1739, 1912, 2051, 2066
NlaIV	GGNNCC	5 cut(s)	11, 602, 759, 2016, 2226
NmeAIII	GCCGAG	1 cut(s)	2422
NmuCI	GTSAC	4 cut(s)	350, 478, 874, 1032
NspI	RCATGY	1 cut(s)	358
NspV	TTCGAA	1 cut(s)	485
PceI	AGGCCT	1 cut(s)	824
PctI	GAATGC	3 cut(s)	46, 344, 543
PdmI	GAANNNNTTC	2 cut(s)	2176, 2279
PfeI	GAWTC	4 cut(s)	470, 1462, 1651, 2149
PflFI	GACNNNGTC	3 cut(s)	903, 1319, 1778
PfoI	TCCNGGA	1 cut(s)	171
PleI	GAGTC	7 cut(s)	95, 218, 385, 538, 660, 1734, 1908
PpsI	GAGTC	7 cut(s)	95, 218, 385, 538, 660, 1734, 1908
Ppu21I	YACGTR	1 cut(s)	564
PpuMI	RGGWCCY	1 cut(s)	601
PshBI	ATTAAT	2 cut(s)	1077, 1419
Psp124BI	GAGCTC	1 cut(s)	303
Psp5II	RGGWCCY	1 cut(s)	601
Psp6I	CCWGG	3 cut(s)	171, 603, 655
PspGI	CCWGG	3 cut(s)	171, 603, 655
PspN4I	GGNNCC	5 cut(s)	11, 602, 759, 2016, 2226
PspPI	GGNCC	2 cut(s)	601, 757
PspPPI	RGGWCCY	1 cut(s)	601
PstI	CTGCAG	1 cut(s)	2444
PsyI	GACNNNGTC	3 cut(s)	903, 1319, 1778
RsaI	GTAC	4 cut(s)	410, 433, 1569, 1636
RsaNI	GTAC	4 cut(s)	409, 432, 1568, 1635
SacI	GAGCTC	1 cut(s)	303
SaqAI	TTAA	5 cut(s)	1077, 1419, 1491, 2256, 2316
Sau3AI	GATC	2 cut(s)	616, 2020
Sau96I	GGNCC	2 cut(s)	601, 757
ScaI	AGTACT	1 cut(s)	1569
SchI	GAGTC	7 cut(s)	95, 219, 386, 539, 661, 1734, 1909
ScrFI	CCNGG	3 cut(s)	173, 605, 657
SduI	GDGCHC	2 cut(s)	303, 2160
SfaNI	GCATC	6 cut(s)	375, 453, 706, 1302, 1651, 1843
SfcI	CTRYAG	3 cut(s)	1641, 2000, 2440
SfuI	TTCGAA	1 cut(s)	485
SinI	GGWCC	1 cut(s)	601
SmlI	CTYRAG	1 cut(s)	2214
SmoI	CTYRAG	1 cut(s)	2214
SseBI	AGGCCT	1 cut(s)	824
SsiI	CCGC	6 cut(s)	240, 342, 646, 687, 1275, 1800
SspI	AATATT	1 cut(s)	1501
SspMI	CTAG	1 cut(s)	623
SstI	GAGCTC	1 cut(s)	303
StuI	AGGCCT	1 cut(s)	824
StyD4I	CCNGG	3 cut(s)	171, 603, 655
StyI	CCWWGG	1 cut(s)	2047
TaaI	ACNGT	4 cut(s)	413, 492, 1645, 2313
TaiI	ACGT	4 cut(s)	566, 1149, 1406, 2348
TaqI	TCGA	5 cut(s)	485, 547, 1222, 1677, 1948
TatI	WGTACW	2 cut(s)	408, 1567
TfiI	GAWTC	4 cut(s)	470, 1462, 1651, 2149
Tru1I	TTAA	5 cut(s)	1077, 1419, 1491, 2256, 2316
Tru9I	TTAA	5 cut(s)	1077, 1419, 1491, 2256, 2316
TscAI	CASTG	6 cut(s)	301, 1041, 1216, 1650, 2270, 2290
TseFI	GTSAC	4 cut(s)	350, 478, 874, 1032
Tsp45I	GTSAC	4 cut(s)	350, 478, 874, 1032
TspDTI	ATGAA	9 cut(s)	11, 350, 650, 656, 860, 1724, 1902, 1925, 2222
TspGWI	ACGGA	1 cut(s)	259
TspRI	CASTG	6 cut(s)	301, 1041, 1216, 1650, 2270, 2290
Tth111I	GACNNNGTC	3 cut(s)	903, 1319, 1778
VpaK11BI	GGWCC	1 cut(s)	601
VspI	ATTAAT	2 cut(s)	1077, 1419
XagI	CCTNNNNNAGG	2 cut(s)	221, 2372
XapI	RAATTY	4 cut(s)	628, 763, 1393, 2175
XceI	RCATGY	1 cut(s)	358
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1261
XmiI	GTMKAC	1 cut(s)	1925
XmnI	GAANNNNTTC	2 cut(s)	2176, 2279
XspI	CTAG	1 cut(s)	623
ZrmI	AGTACT	1 cut(s)	1569

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.