Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0010490

Physical Location & Seq

Genomic Coordinates Forward (+)

1130197 .. 1132758

Gene Structure 2562 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g01340.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2562 bp

ATGAAATTTGGATTTGACGTTCAGCCCTCTGCAGTTTTAAACCCTGGGTTCATGATAGAATACAGACTCCGAAAGTTGAAAATGGCAAGGCATGTCTACCTTCATCGGAAATCCATATTGAATGCCCTCAACAAACCCCAAAATCCAATCTCAGTCCCTCTCTCCTTCAATCTCTGCACTGACTCATTTGCCAACCAAACCCATAAACGACAGATTGAAGACCCAACAGAGAACCACAACCCCACCAACCTCCAAGAGCAACAACGCCATGAGCAAGTCCGAAAGCTCCGAGTCCTCCTCCAGCAGGGTCGCTCTGAAACTGCACGGAGGCTCATCAAGTTTCTCATTCTCCCCGATTCACCCTTCTCTTCCCCGACTGAGCTCTACACCTTGTTTTCTCTCTCTGCACCCTCCATGAAACCCACATTCTGTGACATGCTTTTAGCAGCTTGTTCAGAGTCTAAGATGCCGAATGAAGCAGTAGAATTGTACAGTTTGATGAGGAAAAGTGATACGCGCCCTTGCTTAGCTTCTCTCAATGTGATGCTTGAATGTTTGGTGACTTCAAAACAGTTTGGCAAGACCCTTGAAGTGTTTTCGGAGATTTTTGAGTCGGGTCAGGGCATTCGACCTGATAAGTTCACGTATGGGAAGGCAATACAAGCTTCTGTAAAGTTAGGGGACCTGGAAAGGGCCGGTCAGCTAGTGAATTCAATGAAGATAAAGCGGATGAGTCCAGGTGTGTTTGTGTATAATGTTTTGCTCAGTGGGTTGTGTAAAGAGAAGAAAATGAGAGACGCACAGAAGGTGTTTGATGAGATGGTTGAGGGAAAGGTGGCCCCAAATTTGGTTACGTACAATACGATGATTGATGGGTATTGTAAGGCTGGTGAGTTGGAGAAGGCCTTTGAATTGAGAGAGCGGATGAAGGATGAGAATGTGGAAGCTAATATGGTCACATATAATACAATGCTTAGTGGACTTTGTCGGGTGAAGAGAATGGAGGACGCGAAAAGGATTTTGGAAGAGATGGAAGCTCACGGCTTTGTGCCTGATGGTTTTACTTATAGTATACTCTTTGATGGGCATTTCAGGTGTGGGGATGGTGAGGGGTCACTGGCTTTATTTGAAGAAGCAAACAGGAAAGGTGTGAGGATTAATAGTTATACTTGTAGCATTTTTTTGAATGGCTTGTGCAAGGAAGGAAACATTGAGAAGGCAGAGGACGTTTTGAAGAAACTAATGGAAACTGGTTTTACTCCAGATGTAGTGGTATATAATACTATCGTCAATGGTTATTGTCGAAGAGGTGAGATAGACAAAGCCATCTTTGCAATTGAACAAATGGAAGTTCGCGGGTTGAAGCCGAATTGCATCACCTTCAATTCTTTGATTGACAAGTTCTGTGAGACAAAGGATATGGATATGGCTGAGGAATGGGTGAAGAAGATGGCAAAGAAGGGAGTTATTCCAAATTTGGAGACGTATAACATCCTTATTAATGGCTACGGGCAGATGTGCGTATTTGATAAGTGTTTCCAGATTCTTGAAGTGATGGAAAATAAGGGGATTAAGCCAAATATTGTGAGCTATGGTTCTCTTGTAAATGGTTTATGCAAAGACGGGAGGCTTCTTGAAGCTGAGATAGTACTGAGGGACATGGTAAGCAGAGGGGTTTTGCCAAATGCACAAATATATAATATGCTCATTGGTGGTAGCTGTACAGTGGGGAACCTGAAAGATGCTTTCAGATTCTTCGATGAGATGGCGAAAACTGGGATAAGTCCAACACTTGTAACCTACAATGCACTCATTCATGGACTCTGCAAGAAAGGACGGGTGATGGAAGCTGAAGATTATGTCTCTCAAATTACAAGTAGCTGTTATAGCCCGGATGTGATTACATACAACTCCCTAATTTCAGGTTATTCTGATGCAGGAAACACCCAGAAATGTCTGGAATTATATGAAACGATCAAGAGTCTGGGCATGAAACCTACTTTGTATACATATCATCCTCTAATTAGTGGATGTAGCAGGGAAGATATGGCATTGGCAGAGAATCTGTATAGTGAAATGCTACAGATGGGCTTGGTTCCTGATCGGATTGTATATAATGCACTCATTCACGGTTACGCAGAGCACGGAGACATTCAAAAGGCACTTGCTTTGCACTCTGAGATGGTAAACCAGAAGATTTATGTTGACAGGATGACATATAATAGCTTGATTCTGGGGCACTTCAAGCAAGGAAAAATTTCAGAGGTAAGGGACTTAGTAAATGATATGAAGGCTCAAGGACTGGCTCCTAAAGCTGATACGTATAACTTACTTGTTAAGGGACACTGCGAGCTGAAGGACTTCAGTGGTGCATATTTTTGGTATAGGGAACTGTTTAAGAATGGTTTTCTTCTAAACGCCTCGACGTGCCATGAACTTACATCTGGCCTGCAAAAGGAGGGGAGGTTGCGAGAGGCCGAGATTGTCTGCTCAGAAATGAGCGTCAAAGGATTGAATGACTGCAGTTCTAATGAGGATGTGTTTTCTGTTGCCAATGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0046872 GO:0046914 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

854

Amino Acids

96.17

Weight (kDa)

6.75

Isoelectric Point (pI)

34.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	146 - 274	5.3e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	207 - 256	1.8e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	211 - 260	1.2e-07	PPR repeat family
PPR_1	PF12854	244 - 275	7.2e-09	PPR repeat
PPR_2	PF13041	246 - 295	1.1e-18	PPR repeat family
PPR	PF01535	250 - 276	1.1e-06	PPR repeat
PPR_3	PF13812	251 - 293	7.8e-08	Pentatricopeptide repeat domain
PPR_long	PF17177	263 - 370	4.8e-09	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	267 - 377	1.1e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	280 - 310	3.3e-14	PPR repeat
PPR_2	PF13041	281 - 330	4.2e-20	PPR repeat family
PPR	PF01535	284 - 314	6.6e-11	PPR repeat
PPR_3	PF13812	307 - 359	1.3e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	313 - 345	6e-10	PPR repeat
PPR_2	PF13041	317 - 364	4.1e-16	PPR repeat family
PPR	PF01535	319 - 349	6.1e-08	PPR repeat
PPR_2	PF13041	361 - 400	1.1e-06	PPR repeat family
PPR_3	PF13812	375 - 433	1.3e-08	Pentatricopeptide repeat domain
TPR_24	PF23276	393 - 501	2e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	418 - 450	1.6e-10	PPR repeat
PPR_2	PF13041	421 - 469	1.3e-15	PPR repeat family
PPR_long	PF17177	446 - 536	6.5e-10	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	447 - 506	6.9e-13	Pentatricopeptide repeat domain
PPR_1	PF12854	452 - 484	7.7e-07	PPR repeat
PPR_2	PF13041	456 - 503	2e-13	PPR repeat family
PPR_2	PF13041	491 - 540	2.6e-13	PPR repeat family
PPR_1	PF12854	522 - 555	2.2e-10	PPR repeat
PPR_2	PF13041	526 - 574	8.6e-11	PPR repeat family
PPR	PF01535	529 - 559	3.3e-06	PPR repeat
PPR_3	PF13812	550 - 606	6.6e-14	Pentatricopeptide repeat domain
TPR_24	PF23276	550 - 658	8.3e-07	Fungal tetratrico peptide repeats
PPR_long	PF17177	554 - 674	2.3e-08	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	557 - 589	1.6e-09	PPR repeat
PPR_2	PF13041	561 - 610	4.5e-16	PPR repeat family
PPR_1	PF12854	593 - 619	8.2e-11	PPR repeat
PPR_3	PF13812	631 - 678	2.7e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	631 - 680	2.1e-12	PPR repeat family
PPR	PF01535	634 - 663	6.6e-06	PPR repeat
TPR_24	PF23276	681 - 797	2.5e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	696 - 729	2.2e-07	PPR repeat
PPR_2	PF13041	700 - 744	9.7e-11	PPR repeat family
PPR	PF01535	704 - 730	3.7e-06	PPR repeat
PPR_3	PF13812	725 - 780	2.4e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	737 - 783	2.8e-11	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010490)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12100
fragaria_vesca	FvH4_6g17750 FvH4_6g17750 FvH4_6g17750 FvH4_6g17750
malus_domestica	MD12G1016300.v1.1 MD14G1014400.v1.1
prunus_persica	Prupe.7G119000_v2.0.a1
pyrus_communis	pycom12g01340 pycom14g01210
rosa_chinensis	RchiOBHm_Chr3g0471971
rosa_laevigata	RLG00000024115
rosa_multiflora	Rmu_sc0000090.1_g000022
rosa_roxburghii	Rroxscaffold_6G00409400
rosa_rugosa	Rorug03G0121400
rosa_samantha	Rh3AG172800 Rh3BG198300 Rh3CG189400 Rh3CG196800 Rh3DG176100
rosa_wichuraiana	Rw3G016780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	922
AccI	GTMKAC	3 cut(s)	96, 1072, 2006
AccII	CGCG	3 cut(s)	517, 1010, 1356
AciI	CCGC	3 cut(s)	727, 922, 1356
AcsI	RAATTY	5 cut(s)	5, 709, 844, 1474, 2256
AcuI	CTGAAG	3 cut(s)	1872, 2347, 2375
AfaI	GTAC	4 cut(s)	491, 857, 1650, 1723
AfiI	CCNNNNNNNGG	4 cut(s)	304, 691, 847, 2455
AjiI	CACGTC	1 cut(s)	2427
AjnI	CCWGG	3 cut(s)	43, 684, 736
AjuI	GAANNNNNNNTTGG	2 cut(s)	1004, 1036
AloI	GAACNNNNNNTCC	1 cut(s)	35
Alw21I	GWGCWC	2 cut(s)	384, 2145
Alw26I	GTCTC	5 cut(s)	789, 1403, 1475, 1867, 2142
AoxI	GGCC	5 cut(s)	693, 837, 903, 2446, 2475
ApeKI	GCWGC	1 cut(s)	446
ApoI	RAATTY	5 cut(s)	5, 709, 844, 1474, 2256
AseI	ATTAAT	2 cut(s)	1158, 1500
Asp700I	GAANNNNTTC	2 cut(s)	2257, 2360
AspLEI	GCGC	1 cut(s)	519
AspS9I	GGNCC	3 cut(s)	682, 693, 838
AsuC2I	CCSGG	1 cut(s)	1892
AsuHPI	GGTGA	9 cut(s)	351, 571, 902, 1003, 1118, 1322, 1369, 1453, 1852
AvaII	GGWCC	1 cut(s)	682
BaeGI	GKGCMC	1 cut(s)	2241
BanII	GRGCYC	1 cut(s)	384
BbsI	GAAGAC	1 cut(s)	225
Bbv12I	GWGCWC	2 cut(s)	384, 2145
BbvCI	CCTCAGC	1 cut(s)	1431
BbvI	GCAGC	1 cut(s)	458
BceAI	ACGGC	1 cut(s)	1057
BciT130I	CCWGG	3 cut(s)	45, 686, 738
BcnI	CCSGG	1 cut(s)	1892
BcoDI	GTCTC	5 cut(s)	789, 1403, 1475, 1867, 2142
BfaI	CTAG	1 cut(s)	704
BfmI	CTRYAG	3 cut(s)	30, 2081, 2521
BisI	GCNGC	1 cut(s)	447
BlpI	GCTNAGC	1 cut(s)	526
BlsI	GCNGC	1 cut(s)	448
BmcAI	AGTACT	1 cut(s)	1650
Bme1390I	CCNGG	4 cut(s)	45, 686, 738, 1892
Bme18I	GGWCC	1 cut(s)	682
BmgBI	CACGTC	1 cut(s)	2427
BmgT120I	GGNCC	3 cut(s)	682, 693, 838
BmiI	GGNNCC	5 cut(s)	683, 840, 1733, 2097, 2307
BmrFI	CCNGG	4 cut(s)	45, 686, 738, 1892
BmrI	ACTGGG	1 cut(s)	1785
BmsI	GCATC	5 cut(s)	456, 534, 1383, 1732, 1924
BmuI	ACTGGG	1 cut(s)	1785
BpiI	GAAGAC	1 cut(s)	225
BplI	GAGNNNNNCTC	2 cut(s)	282, 314
BpmI	CTGGAG	2 cut(s)	284, 1245
Bpu10I	CCTNAGC	1 cut(s)	1431
Bpu1102I	GCTNAGC	1 cut(s)	526
BpuEI	CTTGAG	1 cut(s)	2280
BpuMI	CCSGG	1 cut(s)	1892
BsaAI	YACGTR	3 cut(s)	645, 855, 2322
BsaJI	CCNNGG	2 cut(s)	43, 44
BsaXI	ACNNNNNCTCC	2 cut(s)	2450, 2480
Bsc4I	CCNNNNNNNGG	4 cut(s)	304, 691, 847, 2455
Bse118I	RCCGGY	1 cut(s)	695
Bse1I	ACTGG	4 cut(s)	1122, 1255, 1780, 2307
BseBI	CCWGG	3 cut(s)	45, 686, 738
BseDI	CCNNGG	2 cut(s)	43, 44
BseLI	CCNNNNNNNGG	4 cut(s)	304, 691, 847, 2455
BseMII	CTCAG	8 cut(s)	165, 369, 778, 1422, 1632, 1643, 2169, 2505
BseNI	ACTGG	4 cut(s)	1122, 1255, 1780, 2307
BseRI	GAGGAG	1 cut(s)	287
BseSI	GKGCMC	1 cut(s)	2241
BseXI	GCAGC	1 cut(s)	458
BsgI	GTGCAG	3 cut(s)	160, 306, 390
Bsh1236I	CGCG	3 cut(s)	517, 1010, 1356
BshFI	GGCC	5 cut(s)	695, 839, 905, 2448, 2477
BsiHKAI	GWGCWC	2 cut(s)	384, 2145
BsiSI	CCGG	2 cut(s)	696, 1892
BslFI	GGGAC	5 cut(s)	140, 695, 1670, 2285, 2355
BslI	CCNNNNNNNGG	4 cut(s)	304, 691, 847, 2455
BsmAI	GTCTC	5 cut(s)	789, 1403, 1475, 1867, 2142
BsmBI	CGTCTC	2 cut(s)	789, 1475
BsmFI	GGGAC	5 cut(s)	140, 695, 1670, 2285, 2355
BsmI	GAATGC	2 cut(s)	127, 624
BsnI	GGCC	5 cut(s)	695, 839, 905, 2448, 2477
Bsp1286I	GDGCHC	3 cut(s)	384, 2145, 2241
Bsp1407I	TGTACA	2 cut(s)	489, 1721
Bsp143I	GATC	2 cut(s)	1974, 2101
Bsp1720I	GCTNAGC	1 cut(s)	526
BspACI	CCGC	3 cut(s)	727, 922, 1356
BspANI	GGCC	5 cut(s)	695, 839, 905, 2448, 2477
BspCNI	CTCAG	8 cut(s)	164, 370, 777, 1423, 1633, 1644, 2170, 2504
BspFNI	CGCG	3 cut(s)	517, 1010, 1356
BspHI	TCATGA	1 cut(s)	51
BspLI	GGNNCC	5 cut(s)	683, 840, 1733, 2097, 2307
BspMAI	CTGCAG	2 cut(s)	34, 2525
BsrBI	CCGCTC	1 cut(s)	922
BsrFI	RCCGGY	1 cut(s)	695
BsrGI	TGTACA	2 cut(s)	489, 1721
BsrI	ACTGG	4 cut(s)	1122, 1255, 1780, 2307
BssAI	RCCGGY	1 cut(s)	695
BssECI	CCNNGG	2 cut(s)	43, 44
BssMI	GATC	2 cut(s)	1974, 2101
BssNAI	GTATAC	2 cut(s)	1073, 2007
Bst1107I	GTATAC	2 cut(s)	1073, 2007
Bst2UI	CCWGG	3 cut(s)	45, 686, 738
Bst4CI	ACNGT	5 cut(s)	494, 573, 1726, 2132, 2394
Bst6I	CTCTTC	4 cut(s)	373, 989, 1020, 1300
BstAUI	TGTACA	2 cut(s)	489, 1721
BstBAI	YACGTR	3 cut(s)	645, 855, 2322
BstC8I	GCNNGC	2 cut(s)	2351, 2450
BstENI	CCTNNNNNAGG	2 cut(s)	302, 2453
BstFNI	CGCG	3 cut(s)	517, 1010, 1356
BstHHI	GCGC	1 cut(s)	519
BstKTI	GATC	2 cut(s)	1977, 2104
BstMAI	GTCTC	5 cut(s)	789, 1403, 1475, 1867, 2142
BstMBI	GATC	2 cut(s)	1974, 2101
BstMWI	GCNNNNNNNGC	5 cut(s)	662, 1331, 1887, 2245, 2312
BstNI	CCWGG	3 cut(s)	45, 686, 738
BstNSI	RCATGY	2 cut(s)	95, 439
BstSCI	CCNGG	4 cut(s)	43, 684, 736, 1890
BstSFI	CTRYAG	3 cut(s)	30, 2081, 2521
BstSLI	GKGCMC	1 cut(s)	2241
BstSNI	TACGTA	2 cut(s)	855, 2322
BstUI	CGCG	3 cut(s)	517, 1010, 1356
BstV1I	GCAGC	1 cut(s)	458
BstV2I	GAAGAC	1 cut(s)	225
BstZ17I	GTATAC	2 cut(s)	1073, 2007
BsuRI	GGCC	5 cut(s)	695, 839, 905, 2448, 2477
BtrI	CACGTC	1 cut(s)	2427
BtsI	GCAGTG	1 cut(s)	2344
BtsIMutI	CAGTG	6 cut(s)	177, 772, 1115, 1731, 2344, 2371
Cac8I	GCNNGC	2 cut(s)	2351, 2450
CciI	TCATGA	1 cut(s)	51
CfoI	GCGC	1 cut(s)	519
Cfr10I	RCCGGY	1 cut(s)	695
Cfr13I	GGNCC	3 cut(s)	682, 693, 838
CseI	GACGC	3 cut(s)	806, 1016, 2491
Csp6I	GTAC	4 cut(s)	490, 856, 1649, 1722
CviAII	CATG	9 cut(s)	52, 92, 269, 415, 436, 1660, 1817, 1990, 2432
CviQI	GTAC	4 cut(s)	490, 856, 1649, 1722
DpnI	GATC	2 cut(s)	1976, 2103
DpnII	GATC	2 cut(s)	1974, 2101
DraI	TTTAAA	1 cut(s)	39
Eam1104I	CTCTTC	4 cut(s)	373, 989, 1020, 1300
EarI	CTCTTC	4 cut(s)	373, 989, 1020, 1300
Ecl136II	GAGCTC	1 cut(s)	382
Eco105I	TACGTA	2 cut(s)	855, 2322
Eco147I	AGGCCT	1 cut(s)	905
Eco24I	GRGCYC	1 cut(s)	384
Eco47I	GGWCC	1 cut(s)	682
Eco53kI	GAGCTC	1 cut(s)	382
Eco57I	CTGAAG	3 cut(s)	1872, 2347, 2375
EcoICRI	GAGCTC	1 cut(s)	382
EcoNI	CCTNNNNNAGG	2 cut(s)	302, 2453
EcoO109I	RGGNCCY	1 cut(s)	682
EcoRI	GAATTC	1 cut(s)	709
EcoRII	CCWGG	3 cut(s)	43, 684, 736
EcoT38I	GRGCYC	1 cut(s)	384
Esp3I	CGTCTC	2 cut(s)	789, 1475
FaeI	CATG	9 cut(s)	55, 95, 272, 418, 439, 1663, 1820, 1993, 2435
FaqI	GGGAC	5 cut(s)	140, 695, 1670, 2285, 2355
FatI	CATG	9 cut(s)	51, 91, 268, 414, 435, 1659, 1816, 1989, 2431
FauI	CCCGC	1 cut(s)	1349
FblI	GTMKAC	3 cut(s)	96, 1072, 2006
Fnu4HI	GCNGC	1 cut(s)	447
FriOI	GRGCYC	1 cut(s)	384
Fsp4HI	GCNGC	1 cut(s)	447
FspBI	CTAG	1 cut(s)	704
GlaI	GCGC	1 cut(s)	518
GluI	GCNGC	1 cut(s)	447
GsuI	CTGGAG	2 cut(s)	284, 1245
HaeIII	GGCC	5 cut(s)	695, 839, 905, 2448, 2477
HapII	CCGG	2 cut(s)	696, 1892
HgaI	GACGC	3 cut(s)	806, 1016, 2491
HhaI	GCGC	1 cut(s)	519
Hin1II	CATG	9 cut(s)	55, 95, 272, 418, 439, 1663, 1820, 1993, 2435
Hin6I	GCGC	1 cut(s)	517
HinP1I	GCGC	1 cut(s)	517
HincII	GTYRAC	1 cut(s)	2206
HindII	GTYRAC	1 cut(s)	2206
HindIII	AAGCTT	1 cut(s)	663
HpaII	CCGG	2 cut(s)	696, 1892
HphI	GGTGA	9 cut(s)	351, 571, 902, 1003, 1118, 1322, 1369, 1453, 1852
Hpy166II	GTNNAC	7 cut(s)	97, 642, 980, 1073, 2007, 2188, 2206
Hpy188III	TCNNGA	8 cut(s)	52, 1262, 1540, 1547, 1634, 1958, 1978, 2099
Hpy8I	GTNNAC	7 cut(s)	97, 642, 980, 1073, 2007, 2188, 2206
Hpy99I	CGWCG	1 cut(s)	2428
HpyCH4III	ACNGT	5 cut(s)	494, 573, 1726, 2132, 2394
HpyCH4IV	ACGT	7 cut(s)	18, 644, 854, 1227, 1484, 2321, 2426
HpyF10VI	GCNNNNNNNGC	5 cut(s)	662, 1331, 1887, 2245, 2312
HpySE526I	ACGT	7 cut(s)	18, 644, 854, 1227, 1484, 2321, 2426
Hsp92II	CATG	9 cut(s)	55, 95, 272, 418, 439, 1663, 1820, 1993, 2435
HspAI	GCGC	1 cut(s)	517
Kzo9I	GATC	2 cut(s)	1974, 2101
LmnI	GCTCC	2 cut(s)	291, 2311
Lsp1109I	GCAGC	1 cut(s)	458
LweI	GCATC	5 cut(s)	456, 534, 1383, 1732, 1924
MaeI	CTAG	1 cut(s)	704
MaeII	ACGT	7 cut(s)	18, 644, 854, 1227, 1484, 2321, 2426
MaeIII	GTNAC	7 cut(s)	431, 559, 850, 955, 1113, 1795, 2132
MalI	GATC	2 cut(s)	1976, 2103
MbiI	CCGCTC	1 cut(s)	922
MboI	GATC	2 cut(s)	1974, 2101
MfeI	CAATTG	1 cut(s)	1335
MhlI	GDGCHC	3 cut(s)	384, 2145, 2241
MlyI	GAGTC	8 cut(s)	60, 176, 300, 467, 620, 742, 1815, 1990
MmeI	TCCRAC	2 cut(s)	876, 1811
MroXI	GAANNNNTTC	2 cut(s)	2257, 2360
MseI	TTAA	6 cut(s)	38, 1158, 1500, 1572, 2337, 2397
MspI	CCGG	2 cut(s)	696, 1892
MspR9I	CCNGG	4 cut(s)	45, 686, 738, 1892
MunI	CAATTG	1 cut(s)	1335
Mva1269I	GAATGC	2 cut(s)	127, 624
MvaI	CCWGG	3 cut(s)	45, 686, 738
MvnI	CGCG	3 cut(s)	517, 1010, 1356
MwoI	GCNNNNNNNGC	5 cut(s)	662, 1331, 1887, 2245, 2312
NciI	CCSGG	1 cut(s)	1892
NdeII	GATC	2 cut(s)	1974, 2101
NlaIII	CATG	9 cut(s)	55, 95, 272, 418, 439, 1663, 1820, 1993, 2435
NlaIV	GGNNCC	5 cut(s)	683, 840, 1733, 2097, 2307
NmeAIII	GCCGAG	1 cut(s)	2503
NmuCI	GTSAC	4 cut(s)	431, 559, 955, 1113
NspI	RCATGY	2 cut(s)	95, 439
PagI	TCATGA	1 cut(s)	51
PasI	CCCWGGG	1 cut(s)	44
PceI	AGGCCT	1 cut(s)	905
PcsI	WCGNNNNNNNCGW	1 cut(s)	860
PctI	GAATGC	2 cut(s)	127, 624
PdmI	GAANNNNTTC	2 cut(s)	2257, 2360
PfeI	GAWTC	5 cut(s)	356, 1543, 1752, 2062, 2230
PflFI	GACNNNGTC	1 cut(s)	984
PkrI	GCNGC	1 cut(s)	448
PleI	GAGTC	8 cut(s)	60, 176, 299, 466, 619, 741, 1815, 1989
PpsI	GAGTC	8 cut(s)	60, 176, 299, 466, 619, 741, 1815, 1989
Ppu21I	YACGTR	3 cut(s)	645, 855, 2322
PpuMI	RGGWCCY	1 cut(s)	682
PshBI	ATTAAT	2 cut(s)	1158, 1500
Psp124BI	GAGCTC	1 cut(s)	384
Psp5II	RGGWCCY	1 cut(s)	682
Psp6I	CCWGG	3 cut(s)	43, 684, 736
PspGI	CCWGG	3 cut(s)	43, 684, 736
PspN4I	GGNNCC	5 cut(s)	683, 840, 1733, 2097, 2307
PspPI	GGNCC	3 cut(s)	682, 693, 838
PspPPI	RGGWCCY	1 cut(s)	682
PstI	CTGCAG	2 cut(s)	34, 2525
PsyI	GACNNNGTC	1 cut(s)	984
RsaI	GTAC	4 cut(s)	491, 857, 1650, 1723
RsaNI	GTAC	4 cut(s)	490, 856, 1649, 1722
SacI	GAGCTC	1 cut(s)	384
SaqAI	TTAA	6 cut(s)	38, 1158, 1500, 1572, 2337, 2397
SatI	GCNGC	1 cut(s)	447
Sau3AI	GATC	2 cut(s)	1974, 2101
Sau96I	GGNCC	3 cut(s)	682, 693, 838
ScaI	AGTACT	1 cut(s)	1650
SchI	GAGTC	8 cut(s)	60, 176, 300, 467, 620, 742, 1815, 1990
ScrFI	CCNGG	4 cut(s)	45, 686, 738, 1892
SduI	GDGCHC	3 cut(s)	384, 2145, 2241
SfaNI	GCATC	5 cut(s)	456, 534, 1383, 1732, 1924
SfcI	CTRYAG	3 cut(s)	30, 2081, 2521
SinI	GGWCC	1 cut(s)	682
SmlI	CTYRAG	1 cut(s)	2295
SmoI	CTYRAG	1 cut(s)	2295
SnaBI	TACGTA	2 cut(s)	855, 2322
SseBI	AGGCCT	1 cut(s)	905
SsiI	CCGC	3 cut(s)	727, 922, 1356
SspI	AATATT	1 cut(s)	1582
SspMI	CTAG	1 cut(s)	704
SstI	GAGCTC	1 cut(s)	384
StuI	AGGCCT	1 cut(s)	905
StyD4I	CCNGG	4 cut(s)	43, 684, 736, 1890
TaaI	ACNGT	5 cut(s)	494, 573, 1726, 2132, 2394
TaiI	ACGT	7 cut(s)	21, 647, 857, 1230, 1487, 2324, 2429
TaqI	TCGA	4 cut(s)	628, 1303, 1758, 2423
TatI	WGTACW	3 cut(s)	489, 1648, 1721
TfiI	GAWTC	5 cut(s)	356, 1543, 1752, 2062, 2230
Tru1I	TTAA	6 cut(s)	38, 1158, 1500, 1572, 2337, 2397
Tru9I	TTAA	6 cut(s)	38, 1158, 1500, 1572, 2337, 2397
TscAI	CASTG	6 cut(s)	184, 772, 1122, 1731, 2351, 2371
TseFI	GTSAC	4 cut(s)	431, 559, 955, 1113
TseI	GCWGC	1 cut(s)	446
Tsp45I	GTSAC	4 cut(s)	431, 559, 955, 1113
TspGWI	ACGGA	2 cut(s)	340, 2160
TspRI	CASTG	6 cut(s)	184, 772, 1122, 1731, 2351, 2371
Tth111I	GACNNNGTC	1 cut(s)	984
VpaK11BI	GGWCC	1 cut(s)	682
VspI	ATTAAT	2 cut(s)	1158, 1500
XagI	CCTNNNNNAGG	2 cut(s)	302, 2453
XapI	RAATTY	5 cut(s)	5, 709, 844, 1474, 2256
XceI	RCATGY	2 cut(s)	95, 439
XmiI	GTMKAC	3 cut(s)	96, 1072, 2006
XmnI	GAANNNNTTC	2 cut(s)	2257, 2360
XspI	CTAG	1 cut(s)	704
ZrmI	AGTACT	1 cut(s)	1650

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.