Rroxscaffold_6G00409400

Pentatricopeptide repeat-containing protein At5g12100

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0010490
Physical Location & Seq
Reverse (-)
31901361 .. 31904573
3213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00409400.1

Sequence Viewer

Length: 2430 bp
ATGGCAAGGCGCTTCTCTCTTCTTCTTCCCAAGTCTCTACCCACCAAAACCCAAAACCTCCGATCAGTTTCTCTCTGTACAGACTCCGACCCAAGAATCCAACCCTCCAATTCAAACCACCAAGACCAGCAACGCCATGAACAAGTCCGCAAGCTCCGAATCCTCCTCCAGCAGGGCCGAACCGACACCGCAAATCGCTTCATCAAGTCCCTCATTCTCCCCACCTCACCTTTCTCCTCCCCCTCCGACCTCTACAACCTCTTCTCCCTCTCCGCTCCCTCCATGACACCCACATTCTCCGACATGCTCCTCACGGCGTGCACGGAGTCCAAGATGCCCAGCGAAGCAGTTGAGTTGTATGGTCTGATGAGAAAGAGCGACCTCCGCCCGAGTTTGGCCACTCTCAATATGCTGCTAGAGTGCTTGGTGGCTTCGAAAATGTTTAGAAAAACCCTTGAGTTGTTTTCGCAGATTTTCGAGTCTGGTAAGGGCATTCGGCCGGATAAGTTCACTTACGGCAAGGCGGTGCAGGCGGCGGTGAAGCTGGGGGACTTGAATAGGGCTCTTGAGCTTGTGGAGTGCATGAAGAGAGAGAGGTTGAGTCCGAATGTGTTCATTTATAATGTTTTGATTAGTGGGTTGTGTAAAGAGAAGAAAATGAGGGATGCAGAGAAGGTGTTCGATGAAATGCGTGAGAGAAGGGGAGTGCCGACTTTGGTTACTTACAACACGTTGATCGATGGGTATTGTAAGGTGGGTGAGTTGGAGAAGGCGTTTGGGTTGAGAGAGTGGATGAAGGATCAGAAGGTGGAAGCGAATGTGGTGACTTATAATACAATGCTTAATGGGCTTTGTAAGGCAAAGAGAATGGAGGAAGCGAAAACGGTGTTGGAAGAGATGGAGGCTCATGGATTTGTGCCGGATGGTTTTACTTATAGTATACTTTTTGATGGACATCTGAGGTGTGGTGATGATCAGGGCGTGTTGGCTTTGTTTGAAGAAGCAACCAGGAAAGGTGTGAGGATCAATGGGTATACTTGTAGTATTCTGTTGAATGGGTTGTGCAAGAAAGGAAAGATAGAGAAAGCGGAGGAGATTTTGAAGAAATTAATGGAAACTGGTTTTGTTCCAGATGAAGTAGTGTTTAATACTCTGGTGAGTGGATATTGTCGACAATCTGACATTGACAGAGCCATTTTGGTCATTGAGAAAATGGAAGCTCGTGGGTTGAGGCCAAATTGCATCACTTTCAATTCTTTGATTCACAAATTCTGTGAGACAAAGGATATGGATAGGGCGGAGGAATGGGTACAGAAGATGGTAAAGAAGGGAGATTTGCCGAATTTGGAGACGTATAACACCCTTATTAATGGCTATGGGCAGATTTGCGCATTTGATAAGTGTTTCCAGATTCTTGAAGACATGGAAAGTAAGGCAATTAAACCAAATGTTGTGAGCTATGGTTCCCTTATAAATTGTTTATGCAAGGATGGGAGACTTCTTGAAGCTGAAATACAACTCAGAGACATGGTAGACCGAGGGGTTTTGCCAAATGCACAGATATATAATATGCTCATTGATGGTAGCTCTACAGAGGGGAAGCTGAAAGATGCTTTCAGATTTTTTGATGAGATGGCAAAAAATGGGATAAGTCCAACACTTGCAACCTACAATGCAATCATACATGGACTCTGCAAGAAAGGAAGGATGATTGAAGCTGAAGATTATGTGTCTCAAATTACAAGCAGTGGTTATAGACCCGATGTCATCACATACAACTCCCTAATTTCAGGTTATTCTGATACAATAAACACCCAGAAATGTCTGGAATTATATGAAACTATGAAGAATCTGGGCATCAAACCTACTTTGAATACATATCATCCTCTAATTAGTGGATGTAGCAGGGACAATATGGTATTAGCAGAAAAGTTGTTTAATGAGATGTTACAAATGGGCTTGCATCCTGATCGAATCATATATAATGCACTCATCCATGGTTATGCAGAGCATGGAGATACTCAGAAGGCACTTTCTTTGCACTGTGAGATGGTAAACCAGAAAATCAATGTTGACAAGATGACGTATAATAGCTTAATTCTTGGGCACTTCAAACAAGGGAAGGTGTCAGAGGTCAAGGACCTCGTCGATGATATGAAGGCTAAAGGATTGGCACCTAAAGCTGATACATATAACTTACTTGTGAAGGGACACTGTGAACTAAAGGACTTCAGTGGCGCATATTTTTGGTACAGGGAACTGGTTGAGAGTGGTTATCTTCTAAACGTCTCCACATGCAACGAGCTTACAACTGGCCTCCAAAAGGAGGGGAGGTTTCAAGAGGTCCAGGTTATCTGCTTAGAAATGAGTGCTAAAGGAATGGATGATGTTAGCTCTAATGAAGATGCAATTTCTGTTGCCAAGGTGTAG

Protein Analysis

809

Amino Acids

91.32

Weight (kDa)

6.89

Isoelectric Point (pI)

32.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 102 - 230 2e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 163 - 212 1.7e-08 Pentatricopeptide repeat domain
PPR_long PF17177 175 - 336 3.4e-13 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 200 - 231 5.3e-12 PPR repeat
PPR_2 PF13041 202 - 242 3e-12 PPR repeat family
PPR PF01535 206 - 233 1.8e-08 PPR repeat
PPR_3 PF13812 210 - 249 4.2e-06 Pentatricopeptide repeat domain
TPR_24 PF23276 223 - 334 8.2e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 236 - 265 9.7e-11 PPR repeat
PPR_2 PF13041 237 - 286 5.1e-20 PPR repeat family
PPR PF01535 240 - 263 1.4e-06 PPR repeat
PPR_3 PF13812 265 - 315 2.6e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 270 - 301 1.2e-11 PPR repeat
PPR_2 PF13041 273 - 319 2e-17 PPR repeat family
PPR PF01535 275 - 305 4.5e-09 PPR repeat
PPR_2 PF13041 315 - 356 2.8e-06 PPR repeat family
PPR_long PF17177 322 - 442 6.4e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 331 - 389 1.7e-07 Pentatricopeptide repeat domain
TPR_24 PF23276 347 - 460 7.9e-08 Fungal tetratrico peptide repeats
PPR_1 PF12854 374 - 406 2.4e-09 PPR repeat
PPR_2 PF13041 377 - 425 1.9e-13 PPR repeat family
PPR_3 PF13812 401 - 460 1.4e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 408 - 441 2.6e-07 PPR repeat
PPR_2 PF13041 415 - 459 4.2e-11 PPR repeat family
PPR_long PF17177 427 - 499 5e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 447 - 496 3.5e-14 PPR repeat family
PPR_1 PF12854 480 - 510 3.1e-08 PPR repeat
PPR_2 PF13041 482 - 528 4.4e-10 PPR repeat family
PPR_3 PF13812 507 - 562 2.7e-13 Pentatricopeptide repeat domain
PPR_long PF17177 510 - 631 3.2e-10 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 513 - 545 2.9e-08 PPR repeat
PPR_2 PF13041 517 - 566 1.3e-15 PPR repeat family
TPR_24 PF23276 520 - 614 1e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 548 - 575 5.6e-08 PPR repeat
PPR_2 PF13041 552 - 600 4.7e-12 PPR repeat family
PPR_3 PF13812 582 - 634 8.3e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 587 - 636 9.8e-12 PPR repeat family
TPR_24 PF23276 638 - 753 1.7e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 652 - 685 1.2e-06 PPR repeat
PPR_2 PF13041 656 - 700 5e-11 PPR repeat family
PPR PF01535 660 - 686 1.7e-06 PPR repeat
PPR_3 PF13812 682 - 736 2e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 693 - 739 1.3e-11 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010490)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12100
fragaria_vesca FvH4_6g17750 FvH4_6g17750 FvH4_6g17750 FvH4_6g17750
malus_domestica MD12G1016300.v1.1 MD14G1014400.v1.1
prunus_persica Prupe.7G119000_v2.0.a1
pyrus_communis pycom12g01340 pycom14g01210
rosa_chinensis RchiOBHm_Chr3g0471971
rosa_laevigata RLG00000024115
rosa_multiflora Rmu_sc0000090.1_g000022
rosa_roxburghii Rroxscaffold_6G00409400
rosa_rugosa Rorug03G0121400
rosa_samantha Rh3AG172800 Rh3BG198300 Rh3CG189400 Rh3CG196800 Rh3DG176100
rosa_wichuraiana Rw3G016780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 621, 831, 1472
Acc16I TGCGCA 1 cut(s) 1390
AccB1I GGYRCC 1 cut(s) 2173
AccBSI CCGCTC 1 cut(s) 275
AccI GTMKAC 4 cut(s) 940, 1034, 1171, 1533
AciI CCGC 9 cut(s) 148, 189, 273, 385, 524, 533, 536, 1088, 1298
AclWI GGATC 2 cut(s) 807, 1031
AcoI YGGCCR 2 cut(s) 396, 497
AcsI RAATTY 2 cut(s) 1268, 1342
AcuI CTGAAG 2 cut(s) 1740, 2215
AdeI CACNNNGTG 1 cut(s) 318
AfaI GTAC 3 cut(s) 79, 1311, 2252
AfiI CCNNNNNNNGG 4 cut(s) 172, 394, 2323, 2326
AflIII ACRYGT 1 cut(s) 729
AhdI GACNNNNNGTC 1 cut(s) 1763
AjnI CCWGG 2 cut(s) 1007, 2346
AjuI GAANNNNNNNTTGG 2 cut(s) 872, 904
Alw21I GWGCWC 1 cut(s) 323
Alw26I GTCTC 7 cut(s) 39, 1271, 1343, 1489, 1518, 1737, 2293
Alw44I GTGCAC 1 cut(s) 319
AlwI GGATC 2 cut(s) 807, 1031
Ama87I CYCGRG 1 cut(s) 388
AoxI GGCC 5 cut(s) 175, 396, 497, 1232, 2314
ApaLI GTGCAC 1 cut(s) 319
ApeKI GCWGC 1 cut(s) 412
ApoI RAATTY 2 cut(s) 1268, 1342
AseI ATTAAT 2 cut(s) 1109, 1368
Asp700I GAANNNNTTC 2 cut(s) 611, 677
AspLEI GCGC 3 cut(s) 12, 1391, 2240
AspS9I GGNCC 3 cut(s) 175, 2140, 2344
AsuHPI GGTGA 6 cut(s) 219, 550, 770, 835, 980, 1168
AsuII TTCGAA 1 cut(s) 434
AvaI CYCGRG 1 cut(s) 388
AvaII GGWCC 2 cut(s) 2140, 2344
BaeGI GKGCMC 2 cut(s) 323, 2109
BalI TGGCCA 1 cut(s) 398
BanI GGYRCC 1 cut(s) 2173
BanII GRGCYC 1 cut(s) 565
BarI GAAGNNNNNNTAC 2 cut(s) 1497, 1529
BauI CACGAG 1 cut(s) 1221
BbsI GAAGAC 1 cut(s) 1425
Bbv12I GWGCWC 1 cut(s) 323
BbvI GCAGC 1 cut(s) 399
BccI CCATC 9 cut(s) 734, 892, 917, 944, 1312, 1484, 1574, 1627, 2044
BceAI ACGGC 2 cut(s) 330, 532
BcgI CGANNNNNNTGC 2 cut(s) 1952, 1986
BciT130I CCWGG 2 cut(s) 1009, 2348
BclI TGATCA 1 cut(s) 973
BcoDI GTCTC 7 cut(s) 39, 1271, 1343, 1489, 1518, 1737, 2293
BfaI CTAG 1 cut(s) 416
BfmI CTRYAG 1 cut(s) 1590
BfoI RGCGCY 1 cut(s) 13
BisI GCNGC 2 cut(s) 413, 534
BlsI GCNGC 2 cut(s) 414, 535
Bme1390I CCNGG 2 cut(s) 1009, 2348
Bme18I GGWCC 2 cut(s) 2140, 2344
BmeRI GACNNNNNGTC 1 cut(s) 1763
BmeT110I CYCGRG 1 cut(s) 388
BmgT120I GGNCC 3 cut(s) 175, 2140, 2344
BmiI GGNNCC 2 cut(s) 1465, 2175
BmrFI CCNGG 2 cut(s) 1009, 2348
BmsI GCATC 7 cut(s) 324, 655, 1251, 1600, 1866, 1972, 2395
BpiI GAAGAC 1 cut(s) 1425
BpmI CTGGAG 1 cut(s) 152
Bpu14I TTCGAA 1 cut(s) 434
BpuEI CTTGAG 2 cut(s) 476, 587
Bsa29I ATCGAT 1 cut(s) 738
BsaBI GATNNNNATC 1 cut(s) 954
BsaJI CCNNGG 3 cut(s) 1537, 1996, 2421
BsaXI ACNNNNNCTCC 6 cut(s) 248, 278, 758, 788, 2318, 2348
Bsc4I CCNNNNNNNGG 4 cut(s) 172, 394, 2323, 2326
Bse1I ACTGG 3 cut(s) 1123, 2265, 2317
Bse8I GATNNNNATC 1 cut(s) 954
BseBI CCWGG 2 cut(s) 1009, 2348
BseCI ATCGAT 1 cut(s) 738
BseDI CCNNGG 3 cut(s) 1537, 1996, 2421
BseJI GATNNNNATC 1 cut(s) 954
BseLI CCNNNNNNNGG 4 cut(s) 172, 394, 2323, 2326
BseMII CTCAG 3 cut(s) 950, 1534, 2036
BseNI ACTGG 3 cut(s) 1123, 2265, 2317
BseRI GAGGAG 4 cut(s) 155, 226, 299, 1106
BseSI GKGCMC 2 cut(s) 323, 2109
BseX3I CGGCCG 1 cut(s) 497
BseXI GCAGC 1 cut(s) 399
BseYI CCCAGC 2 cut(s) 338, 544
BsgI GTGCAG 1 cut(s) 548
Bsh1285I CGRYCG 1 cut(s) 500
BshFI GGCC 5 cut(s) 177, 398, 499, 1234, 2316
BshNI GGYRCC 1 cut(s) 2173
BshVI ATCGAT 1 cut(s) 738
BsiEI CGRYCG 1 cut(s) 500
BsiHKAI GWGCWC 1 cut(s) 323
BsiHKCI CYCGRG 1 cut(s) 388
BsiSI CCGG 2 cut(s) 500, 920
BslFI GGGAC 4 cut(s) 193, 563, 1922, 2223
BslI CCNNNNNNNGG 4 cut(s) 172, 394, 2323, 2326
BsmAI GTCTC 7 cut(s) 39, 1271, 1343, 1489, 1518, 1737, 2293
BsmBI CGTCTC 2 cut(s) 1343, 2293
BsmFI GGGAC 4 cut(s) 193, 563, 1922, 2223
BsmI GAATGC 1 cut(s) 492
BsnI GGCC 5 cut(s) 177, 398, 499, 1234, 2316
BsoBI CYCGRG 1 cut(s) 388
Bsp119I TTCGAA 1 cut(s) 434
Bsp1286I GDGCHC 3 cut(s) 323, 565, 2109
Bsp1407I TGTACA 1 cut(s) 77
Bsp143I GATC 6 cut(s) 62, 735, 799, 973, 1023, 1969
Bsp19I CCATGG 1 cut(s) 1996
BspACI CCGC 9 cut(s) 148, 189, 273, 385, 524, 533, 536, 1088, 1298
BspANI GGCC 5 cut(s) 177, 398, 499, 1234, 2316
BspCNI CTCAG 3 cut(s) 951, 1533, 2035
BspDI ATCGAT 1 cut(s) 738
BspLI GGNNCC 2 cut(s) 1465, 2175
BspPI GGATC 2 cut(s) 807, 1031
BspT104I TTCGAA 1 cut(s) 434
BspT107I GGYRCC 1 cut(s) 2173
BsrBI CCGCTC 1 cut(s) 275
BsrGI TGTACA 1 cut(s) 77
BsrI ACTGG 3 cut(s) 1123, 2265, 2317
BssECI CCNNGG 3 cut(s) 1537, 1996, 2421
BssMI GATC 6 cut(s) 62, 735, 799, 973, 1023, 1969
BssNAI GTATAC 2 cut(s) 941, 1035
BssSI CACGAG 1 cut(s) 1221
BssT1I CCWWGG 2 cut(s) 1996, 2421
Bst1107I GTATAC 2 cut(s) 941, 1035
Bst2BI CACGAG 1 cut(s) 1221
Bst2UI CCWGG 2 cut(s) 1009, 2348
Bst4CI ACNGT 3 cut(s) 886, 2045, 2216
Bst6I CTCTTC 4 cut(s) 24, 266, 581, 888
BstAUI TGTACA 1 cut(s) 77
BstBI TTCGAA 1 cut(s) 434
BstC8I GCNNGC 4 cut(s) 152, 319, 531, 1961
BstDEI CTNAG 4 cut(s) 959, 1520, 2022, 2359
BstDSI CCRYGG 1 cut(s) 1996
BstENI CCTNNNNNAGG 2 cut(s) 170, 2321
BstH2I RGCGCY 1 cut(s) 13
BstHHI GCGC 3 cut(s) 12, 1391, 2240
BstKTI GATC 6 cut(s) 65, 738, 802, 976, 1026, 1972
BstMAI GTCTC 7 cut(s) 39, 1271, 1343, 1489, 1518, 1737, 2293
BstMBI GATC 6 cut(s) 62, 735, 799, 973, 1023, 1969
BstMCI CGRYCG 1 cut(s) 500
BstMWI GCNNNNNNNGC 4 cut(s) 384, 530, 847, 2180
BstNI CCWGG 2 cut(s) 1009, 2348
BstNSI RCATGY 2 cut(s) 307, 2298
BstSCI CCNGG 2 cut(s) 1007, 2346
BstSFI CTRYAG 1 cut(s) 1590
BstSLI GKGCMC 2 cut(s) 323, 2109
BstV1I GCAGC 1 cut(s) 399
BstV2I GAAGAC 1 cut(s) 1425
BstZ17I GTATAC 2 cut(s) 941, 1035
BstZI CGGCCG 1 cut(s) 497
Bsu15I ATCGAT 1 cut(s) 738
BsuRI GGCC 5 cut(s) 177, 398, 499, 1234, 2316
BsuTUI ATCGAT 1 cut(s) 738
BtgI CCRYGG 1 cut(s) 1996
BtsI GCAGTG 1 cut(s) 1753
BtsIMutI CAGTG 4 cut(s) 1753, 2041, 2212, 2239
Cac8I GCNNGC 4 cut(s) 152, 319, 531, 1961
CfoI GCGC 3 cut(s) 12, 1391, 2240
Cfr13I GGNCC 3 cut(s) 175, 2140, 2344
ClaI ATCGAT 1 cut(s) 738
Csp6I GTAC 3 cut(s) 78, 1310, 2251
CviQI GTAC 3 cut(s) 78, 1310, 2251
DdeI CTNAG 4 cut(s) 959, 1520, 2022, 2359
DpnI GATC 6 cut(s) 64, 737, 801, 975, 1025, 1971
DpnII GATC 6 cut(s) 62, 735, 799, 973, 1023, 1969
DraIII CACNNNGTG 1 cut(s) 318
DriI GACNNNNNGTC 1 cut(s) 1763
EaeI YGGCCR 2 cut(s) 396, 497
EagI CGGCCG 1 cut(s) 497
Eam1104I CTCTTC 4 cut(s) 24, 266, 581, 888
Eam1105I GACNNNNNGTC 1 cut(s) 1763
EarI CTCTTC 4 cut(s) 24, 266, 581, 888
EciI GGCGGA 2 cut(s) 374, 1313
EclXI CGGCCG 1 cut(s) 497
Eco130I CCWWGG 2 cut(s) 1996, 2421
Eco24I GRGCYC 1 cut(s) 565
Eco47I GGWCC 2 cut(s) 2140, 2344
Eco52I CGGCCG 1 cut(s) 497
Eco57I CTGAAG 2 cut(s) 1740, 2215
Eco88I CYCGRG 1 cut(s) 388
EcoNI CCTNNNNNAGG 2 cut(s) 170, 2321
EcoO109I RGGNCCY 1 cut(s) 2140
EcoRII CCWGG 2 cut(s) 1007, 2346
EcoT14I CCWWGG 2 cut(s) 1996, 2421
EcoT38I GRGCYC 1 cut(s) 565
ErhI CCWWGG 2 cut(s) 1996, 2421
Esp3I CGTCTC 2 cut(s) 1343, 2293
FaqI GGGAC 4 cut(s) 193, 563, 1922, 2223
FbaI TGATCA 1 cut(s) 973
FblI GTMKAC 4 cut(s) 940, 1034, 1171, 1533
Fnu4HI GCNGC 2 cut(s) 413, 534
FriOI GRGCYC 1 cut(s) 565
Fsp4HI GCNGC 2 cut(s) 413, 534
FspBI CTAG 1 cut(s) 416
FspI TGCGCA 1 cut(s) 1390
GlaI GCGC 3 cut(s) 11, 1390, 2239
GluI GCNGC 2 cut(s) 413, 534
GsaI CCCAGC 2 cut(s) 342, 548
GsuI CTGGAG 1 cut(s) 152
HaeII RGCGCY 1 cut(s) 13
HaeIII GGCC 5 cut(s) 177, 398, 499, 1234, 2316
HapII CCGG 2 cut(s) 500, 920
HhaI GCGC 3 cut(s) 12, 1391, 2240
Hin6I GCGC 3 cut(s) 10, 1389, 2238
HinP1I GCGC 3 cut(s) 10, 1389, 2238
HincII GTYRAC 2 cut(s) 1172, 2074
HindII GTYRAC 2 cut(s) 1172, 2074
HpaII CCGG 2 cut(s) 500, 920
HphI GGTGA 6 cut(s) 219, 550, 770, 835, 980, 1168
Hpy166II GTNNAC 9 cut(s) 321, 510, 941, 1035, 1172, 1534, 2056, 2074, 2219
Hpy188III TCNNGA 8 cut(s) 566, 1130, 1408, 1415, 1502, 1826, 1967, 2339
Hpy8I GTNNAC 9 cut(s) 321, 510, 941, 1035, 1172, 1534, 2056, 2074, 2219
Hpy99I CGWCG 1 cut(s) 2150
HpyCH4III ACNGT 3 cut(s) 886, 2045, 2216
HpyCH4IV ACGT 4 cut(s) 731, 1352, 2084, 2286
HpyF10VI GCNNNNNNNGC 4 cut(s) 384, 530, 847, 2180
HpyF3I CTNAG 4 cut(s) 959, 1520, 2022, 2359
HpySE526I ACGT 4 cut(s) 731, 1352, 2084, 2286
HspAI GCGC 3 cut(s) 10, 1389, 2238
Ksp22I TGATCA 1 cut(s) 973
Kzo9I GATC 6 cut(s) 62, 735, 799, 973, 1023, 1969
LmnI GCTCC 3 cut(s) 159, 280, 312
Lsp1109I GCAGC 1 cut(s) 399
LweI GCATC 7 cut(s) 324, 655, 1251, 1600, 1866, 1972, 2395
MaeI CTAG 1 cut(s) 416
MaeII ACGT 4 cut(s) 731, 1352, 2084, 2286
MaeIII GTNAC 3 cut(s) 718, 823, 1947
MalI GATC 6 cut(s) 64, 737, 801, 975, 1025, 1971
MbiI CCGCTC 1 cut(s) 275
MboI GATC 6 cut(s) 62, 735, 799, 973, 1023, 1969
MhlI GDGCHC 3 cut(s) 323, 565, 2109
MlsI TGGCCA 1 cut(s) 398
MluNI TGGCCA 1 cut(s) 398
MlyI GAGTC 5 cut(s) 77, 335, 488, 610, 1683
MmeI TCCRAC 7 cut(s) 111, 124, 270, 324, 744, 870, 1679
Mox20I TGGCCA 1 cut(s) 398
MroXI GAANNNNTTC 2 cut(s) 611, 677
MscI TGGCCA 1 cut(s) 398
MseI TTAA 7 cut(s) 843, 1109, 1146, 1368, 1440, 1938, 2096
MslI CAYNNNNRTG 1 cut(s) 2001
Msp20I TGGCCA 1 cut(s) 398
MspI CCGG 2 cut(s) 500, 920
MspR9I CCNGG 2 cut(s) 1009, 2348
Mva1269I GAATGC 1 cut(s) 492
MvaI CCWGG 2 cut(s) 1009, 2348
MwoI GCNNNNNNNGC 4 cut(s) 384, 530, 847, 2180
NcoI CCATGG 1 cut(s) 1996
NdeII GATC 6 cut(s) 62, 735, 799, 973, 1023, 1969
NlaIV GGNNCC 2 cut(s) 1465, 2175
NmuCI GTSAC 1 cut(s) 823
NsbI TGCGCA 1 cut(s) 1390
NspI RCATGY 2 cut(s) 307, 2298
NspV TTCGAA 1 cut(s) 434
PctI GAATGC 1 cut(s) 492
PdmI GAANNNNTTC 2 cut(s) 611, 677
PfeI GAWTC 6 cut(s) 96, 159, 1261, 1411, 1849, 1974
PflFI GACNNNGTC 1 cut(s) 2144
PkrI GCNGC 2 cut(s) 414, 535
PleI GAGTC 5 cut(s) 77, 334, 487, 609, 1683
PpsI GAGTC 5 cut(s) 77, 334, 487, 609, 1683
PpuMI RGGWCCY 1 cut(s) 2140
PshBI ATTAAT 2 cut(s) 1109, 1368
PsiI TTATAA 3 cut(s) 621, 831, 1472
Psp5II RGGWCCY 1 cut(s) 2140
Psp6I CCWGG 2 cut(s) 1007, 2346
PspFI CCCAGC 2 cut(s) 338, 544
PspGI CCWGG 2 cut(s) 1007, 2346
PspN4I GGNNCC 2 cut(s) 1465, 2175
PspPI GGNCC 3 cut(s) 175, 2140, 2344
PspPPI RGGWCCY 1 cut(s) 2140
PsyI GACNNNGTC 1 cut(s) 2144
RsaI GTAC 3 cut(s) 79, 1311, 2252
RsaNI GTAC 3 cut(s) 78, 1310, 2251
RseI CAYNNNNRTG 1 cut(s) 2001
SalI GTCGAC 1 cut(s) 1170
SaqAI TTAA 7 cut(s) 843, 1109, 1146, 1368, 1440, 1938, 2096
SatI GCNGC 2 cut(s) 413, 534
Sau3AI GATC 6 cut(s) 62, 735, 799, 973, 1023, 1969
Sau96I GGNCC 3 cut(s) 175, 2140, 2344
SchI GAGTC 5 cut(s) 77, 335, 488, 610, 1683
ScrFI CCNGG 2 cut(s) 1009, 2348
SduI GDGCHC 3 cut(s) 323, 565, 2109
SfaNI GCATC 7 cut(s) 324, 655, 1251, 1600, 1866, 1972, 2395
SfcI CTRYAG 1 cut(s) 1590
SfuI TTCGAA 1 cut(s) 434
SinI GGWCC 2 cut(s) 2140, 2344
SmiMI CAYNNNNRTG 1 cut(s) 2001
SmlI CTYRAG 2 cut(s) 455, 566
SmoI CTYRAG 2 cut(s) 455, 566
SsiI CCGC 9 cut(s) 148, 189, 273, 385, 524, 533, 536, 1088, 1298
SspMI CTAG 1 cut(s) 416
StyD4I CCNGG 2 cut(s) 1007, 2346
StyI CCWWGG 2 cut(s) 1996, 2421
TaaI ACNGT 3 cut(s) 886, 2045, 2216
TaiI ACGT 4 cut(s) 734, 1355, 2087, 2289
TaqI TCGA 7 cut(s) 434, 477, 681, 738, 1171, 1972, 2148
TaqII GACCGA 1 cut(s) 1551
TatI WGTACW 1 cut(s) 77
TauI GCSGC 1 cut(s) 536
TfiI GAWTC 6 cut(s) 96, 159, 1261, 1411, 1849, 1974
Tru1I TTAA 7 cut(s) 843, 1109, 1146, 1368, 1440, 1938, 2096
Tru9I TTAA 7 cut(s) 843, 1109, 1146, 1368, 1440, 1938, 2096
TscAI CASTG 4 cut(s) 1753, 2048, 2219, 2239
TseFI GTSAC 1 cut(s) 823
TseI GCWGC 1 cut(s) 412
Tsp45I GTSAC 1 cut(s) 823
TspGWI ACGGA 1 cut(s) 338
TspRI CASTG 4 cut(s) 1753, 2048, 2219, 2239
Tth111I GACNNNGTC 1 cut(s) 2144
VneI GTGCAC 1 cut(s) 319
VpaK11BI GGWCC 2 cut(s) 2140, 2344
VspI ATTAAT 2 cut(s) 1109, 1368
XagI CCTNNNNNAGG 2 cut(s) 170, 2321
XapI RAATTY 2 cut(s) 1268, 1342
XceI RCATGY 2 cut(s) 307, 2298
XmiI GTMKAC 4 cut(s) 940, 1034, 1171, 1533
XmnI GAANNNNTTC 2 cut(s) 611, 677
XspI CTAG 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.