Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0010490

Physical Location & Seq

Genomic Coordinates Forward (+)

1271209 .. 1274576

Gene Structure 3368 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1014400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2481 bp

ATGGCAAGACGTGTTTATCTCCTTCCCAAACCCGTATTGAATGCCATCAACAAACCTCAAAATCCAATCTCAGTCCCTCTATCTTTCAATCTCTGCACAGACTCTGCTGACAACCAAACTCATCAACCACAGATTGAAGGCCCAACAGAGAACCACAACCCCACCAACATCCACGACCAACAACGCCATGAGCAAGTCCGAAAACTCCGAGTCCTTCTCCAGCAGGGTCGCTCTGAAACAGCACGGAGGCTCATCAAGTCTCTCATTCTCCCCGGTTCACCCTTCTCTTCCCCCACTGAACTCTACACCCTGTTTTCTCTCTCTGCACCCTCCATGAAACCCACATTCTGCGACATGCTTTTAGCCGCTTGTTCAGAGTCTAAGATGCCGACTGAAGCAGTAGAATTGTACAGTTTGATGAGGAAAAGTGGTACACGCCCTTGCTTAGCTTCTGTCAATGTGATGCTTGAATCTTTGGTGACTTCAAAGCAGTTTGGTAAGACCCTTGAATTGTTTTCGGAGATTTTCGAGTCAGGTCAGGGCATTCGACCTGATAAGTTCACGTATGGGAAGGCAATACAAGCTTCTGTAAAGTTAGGGGACCTGGAAAGGGCTGGTCAGCTAGTGAATTCAATGAAGATGAAGCGGATGAATCCAGGTGTGTTTGTGTATAATGTTTTACTCGGTGGGCTGTGTAAAGAGAAGAAAATGAGGGACGCACAGAAGGTGTTTGATGAAATGATTGAGGGAAAGGTGACCCCGAATTTGGTTACGTACAATACGATGATTGATGGGTATTCTAAGGCGGGTGAGTTGGAGAAGGCCTTTGAATTGAGAGAGAGGATGAAGGATGAGAATGTGGAAGCTAATATGGTCACATATAATACAATGCTCAGTGGACTTTGTCGGGTGAAGAGAATCGAGGACGCGAAAAGGATTTTGGAAGAGATGGAAGCTCACAGCTTTGCACCTGATGGTTTTACTTATAGCATACTCTTTGATGGGCATTTCAGGTGTGGGGATGGTGAGGGGTCACTGGCTTTATTTGAAGAAGCAATTAGTAAAGGTGTGAGGATTAATGGTTATACTTGTAGCATTTTATTGAATGGCTTGTGCAAGGAAGGAAACATTGAGAAGGCAGAGGAGGTTTTGAAGAAACTAATGGAAACTGGTTTTATTCCAGATGTAGTGGTATATAATACTATTGTCAGTGGTTATTGTCGAAGGGGTGAGATAGACAAAGCCATTTTGGCCATTGAACAAATGGAAGTACGCGGGTTAAAGCCAAACTGCATCACCTTCAATTCTTTGATTGACAAGATCCGTGAGACAAAGGATATGGATATGGCTGAGGAATGGGTGAAGAAGATGGCAAAGAGGGGAGTTTTTCCAAATTTGGAGACGTATAACATCCTTATTAATGGCTACGGGCAGATGGGTGCATTCGATAAGTGTTTCCAGATTGTTGAAGAGATGGAAAATAAGGGGATTAAGCCAAATATTGTGAGCTATGGTTCTCTTGTAAATGGTTTGTGCAAGGACGGGAGGCTTCTTGAAGCTGAAATAGTACTGAGGGACATGCTAAGCAGAGGGGTTTTGCCAAATGCACAAATATATAATATGCTCATTGGTGGTTGCTGTACGGTGGGGAATCTGAAAGATGCTTACAGATTCCTCGATGAGATGGCGAAAACTGGGATAAGTCCAACACTTGTAACCTACAATGCACTCATTCATGGACTCTGCAAGAAAGGACGGTTCATGGAAGCTGAAGACTATGTATCTCAAATTACAAGTAGCGGTTATAGCCCGAATGTGATCACATACAACTCCCTAATTTCAGGTTATTCTGATGCTGGAAACACCCAGAAATGTCTGGAATTGTTTGAAACTATCAAGAGTCTGGGCATGAAACCTACTTTGTATACATATCATCCTCTAATTAGTGGGTGTATCAGGGAAGATATGGTATTAGCAGAGAAGCTATATAATGAAATGCTACAGATGGGCCTGGTTCCTGACCGGGTTGTATATAATGCACTCATTCACGGTTACGCAGAGCACGGAGACACTCAAAAGGCACTTGCTTTGCACTCTGAGATGGTAAACCAGAATGTTTATGTTGACAGGATGACATATAATAGCTTGATTCTGGGGCACTTCAAGCAAGGAAAAATTTCAGAGGTAAAGGATCTGGTAAATGATATGAAGGCTCAAGGATTGACTCCTAAAGCCGATACGTATAGCTTACTTGTTAAGGGACACTGTGAACTGAAGGACTTCAGTGGTGCCTATTTTTGGTACAGGGAACTGTTTGAGAGTGGTTTTCTTCTAAATGCCTCGACGTGCCATGAACTTACATCTGGCCTTCAAAAGGAGGGGAGGTTGCGAGAGGCCGAGATTGTCTGTTCAGAAATGAGCGCCAAAGGAATGAATGACTGCAGCTCTAATGAGGAAGTGTTTTCTGTTGCCAACGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0046872 GO:0046914 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

827

Amino Acids

92.44

Weight (kDa)

6.16

Isoelectric Point (pI)

33.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	180 - 229	8.6e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	184 - 233	2.7e-07	PPR repeat family
PPR_1	PF12854	217 - 247	7.4e-08	PPR repeat
PPR_2	PF13041	219 - 266	4.1e-16	PPR repeat family
PPR	PF01535	223 - 249	3.6e-06	PPR repeat
PPR_3	PF13812	224 - 266	3.8e-07	Pentatricopeptide repeat domain
PPR_long	PF17177	237 - 342	1e-08	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	240 - 350	5.1e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	252 - 283	2.5e-11	PPR repeat
PPR_2	PF13041	254 - 303	6.1e-19	PPR repeat family
PPR	PF01535	257 - 287	5.8e-10	PPR repeat
PPR_3	PF13812	280 - 332	4.7e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	286 - 318	5.8e-10	PPR repeat
PPR_2	PF13041	290 - 337	3.3e-15	PPR repeat family
PPR	PF01535	292 - 320	7e-07	PPR repeat
PPR_2	PF13041	334 - 373	3.7e-06	PPR repeat family
PPR_3	PF13812	348 - 406	2.6e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	355 - 386	9.1e-06	PPR repeat
TPR_24	PF23276	364 - 473	3.9e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	391 - 423	2.3e-10	PPR repeat
PPR_2	PF13041	394 - 439	5.1e-14	PPR repeat family
PPR_long	PF17177	419 - 509	6.4e-11	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	420 - 479	9.2e-12	Pentatricopeptide repeat domain
PPR_2	PF13041	435 - 476	3e-08	PPR repeat family
PPR_1	PF12854	460 - 493	1e-07	PPR repeat
PPR_2	PF13041	464 - 513	1.7e-15	PPR repeat family
PPR	PF01535	468 - 497	3.2e-07	PPR repeat
PPR_long	PF17177	480 - 563	1.9e-08	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	495 - 527	3.7e-10	PPR repeat
PPR_2	PF13041	500 - 547	6.5e-11	PPR repeat family
PPR	PF01535	502 - 532	4.1e-06	PPR repeat
PPR_3	PF13812	523 - 579	8.9e-13	Pentatricopeptide repeat domain
PPR_1	PF12854	530 - 562	1.8e-09	PPR repeat
PPR_2	PF13041	534 - 583	3.8e-16	PPR repeat family
PPR_long	PF17177	552 - 648	3.4e-08	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	566 - 592	7.1e-10	PPR repeat
PPR_2	PF13041	569 - 617	1.4e-13	PPR repeat family
PPR	PF01535	572 - 601	4.9e-06	PPR repeat
PPR_3	PF13812	599 - 651	7.3e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	601 - 630	1.3e-06	PPR repeat
PPR_2	PF13041	604 - 652	1.8e-12	PPR repeat family
PPR	PF01535	607 - 636	2e-06	PPR repeat
PPR_1	PF12854	669 - 702	2.6e-07	PPR repeat
PPR_2	PF13041	673 - 719	1.9e-11	PPR repeat family
PPR	PF01535	676 - 705	2.1e-06	PPR repeat
PPR_3	PF13812	698 - 753	3.5e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	710 - 756	3.1e-11	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010490)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12100
fragaria_vesca	FvH4_6g17750 FvH4_6g17750 FvH4_6g17750 FvH4_6g17750
malus_domestica	MD12G1016300.v1.1 MD14G1014400.v1.1
prunus_persica	Prupe.7G119000_v2.0.a1
pyrus_communis	pycom12g01340 pycom14g01210
rosa_chinensis	RchiOBHm_Chr3g0471971
rosa_laevigata	RLG00000024115
rosa_multiflora	Rmu_sc0000090.1_g000022
rosa_roxburghii	Rroxscaffold_6G00409400
rosa_rugosa	Rorug03G0121400
rosa_samantha	Rh3AG172800 Rh3BG198300 Rh3CG189400 Rh3CG196800 Rh3DG176100
rosa_wichuraiana	Rw3G016780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2288
AccI	GTMKAC	1 cut(s)	1925
AccII	CGCG	2 cut(s)	929, 1275
AciI	CCGC	5 cut(s)	366, 646, 806, 1275, 1800
AclWI	GGATC	2 cut(s)	1315, 2199
AcoI	YGGCCR	1 cut(s)	1251
AcsI	RAATTY	4 cut(s)	628, 763, 1393, 2175
AcuI	CTGAAG	4 cut(s)	414, 1791, 2266, 2294
AfaI	GTAC	7 cut(s)	410, 433, 776, 1272, 1569, 1642, 2303
AfiI	CCNNNNNNNGG	4 cut(s)	610, 766, 2298, 2374
AflIII	ACRYGT	1 cut(s)	10
AjiI	CACGTC	2 cut(s)	11, 2346
AjnI	CCWGG	3 cut(s)	603, 655, 2010
AjuI	GAANNNNNNNTTGG	2 cut(s)	923, 955
Alw21I	GWGCWC	1 cut(s)	2064
Alw26I	GTCTC	4 cut(s)	264, 1322, 1394, 2061
AlwI	GGATC	2 cut(s)	1315, 2199
AlwNI	CAGNNNCTG	1 cut(s)	104
AoxI	GGCC	6 cut(s)	139, 822, 1251, 2008, 2365, 2394
ApeKI	GCWGC	1 cut(s)	2442
ApoI	RAATTY	4 cut(s)	628, 763, 1393, 2175
ArsI	GACNNNNNNTTYG	2 cut(s)	748, 780
AseI	ATTAAT	2 cut(s)	1077, 1419
Asp700I	GAANNNNTTC	2 cut(s)	2176, 2279
AspLEI	GCGC	1 cut(s)	2423
AspS9I	GGNCC	3 cut(s)	140, 601, 2008
AsuC2I	CCSGG	2 cut(s)	273, 2024
AsuHPI	GGTGA	9 cut(s)	270, 490, 766, 821, 922, 1037, 1241, 1288, 1372
AvaII	GGWCC	1 cut(s)	601
BaeGI	GKGCMC	1 cut(s)	2160
BalI	TGGCCA	1 cut(s)	1253
BanI	GGYRCC	1 cut(s)	2288
BbsI	GAAGAC	1 cut(s)	1779
Bbv12I	GWGCWC	1 cut(s)	2064
BbvCI	CCTCAGC	1 cut(s)	1350
BbvI	GCAGC	1 cut(s)	2454
BciT130I	CCWGG	3 cut(s)	605, 657, 2012
BclI	TGATCA	1 cut(s)	1818
BcnI	CCSGG	2 cut(s)	273, 2024
BcoDI	GTCTC	4 cut(s)	264, 1322, 1394, 2061
BfaI	CTAG	2 cut(s)	623, 2479
BfmI	CTRYAG	2 cut(s)	2000, 2440
BfoI	RGCGCY	1 cut(s)	2424
BglI	GCCNNNNNGGC	1 cut(s)	1250
BisI	GCNGC	2 cut(s)	366, 2443
BlpI	GCTNAGC	2 cut(s)	445, 1583
BlsI	GCNGC	2 cut(s)	367, 2444
BmcAI	AGTACT	1 cut(s)	1569
Bme1390I	CCNGG	5 cut(s)	273, 605, 657, 2012, 2024
Bme18I	GGWCC	1 cut(s)	601
BmgBI	CACGTC	2 cut(s)	11, 2346
BmgT120I	GGNCC	3 cut(s)	140, 601, 2008
BmiI	GGNNCC	3 cut(s)	602, 2016, 2290
BmrFI	CCNGG	5 cut(s)	273, 605, 657, 2012, 2024
BmrI	ACTGGG	1 cut(s)	1704
BmsI	GCATC	5 cut(s)	375, 453, 1302, 1651, 1843
BmuI	ACTGGG	1 cut(s)	1704
BpiI	GAAGAC	1 cut(s)	1779
BplI	GAGNNNNNCTC	2 cut(s)	201, 233
BpmI	CTGGAG	1 cut(s)	203
Bpu10I	CCTNAGC	1 cut(s)	1350
Bpu1102I	GCTNAGC	2 cut(s)	445, 1583
BpuEI	CTTGAG	1 cut(s)	2199
BpuMI	CCSGG	2 cut(s)	273, 2024
BsaAI	YACGTR	3 cut(s)	564, 774, 2241
BsaJI	CCNNGG	1 cut(s)	271
BsaXI	ACNNNNNCTCC	2 cut(s)	2369, 2399
Bsc4I	CCNNNNNNNGG	4 cut(s)	610, 766, 2298, 2374
Bse1I	ACTGG	3 cut(s)	1041, 1174, 1699
BseBI	CCWGG	3 cut(s)	605, 657, 2012
BseDI	CCNNGG	1 cut(s)	271
BseGI	GGATG	8 cut(s)	168, 654, 849, 856, 1027, 1410, 1933, 2136
BseLI	CCNNNNNNNGG	4 cut(s)	610, 766, 2298, 2374
BseMII	CTCAG	5 cut(s)	84, 907, 1341, 1562, 2088
BseNI	ACTGG	3 cut(s)	1041, 1174, 1699
BseRI	GAGGAG	1 cut(s)	1157
BseSI	GKGCMC	1 cut(s)	2160
BseXI	GCAGC	1 cut(s)	2454
BsgI	GTGCAG	2 cut(s)	79, 309
Bsh1236I	CGCG	2 cut(s)	929, 1275
BshFI	GGCC	6 cut(s)	141, 824, 1253, 2010, 2367, 2396
BshNI	GGYRCC	1 cut(s)	2288
BsiHKAI	GWGCWC	1 cut(s)	2064
BsiSI	CCGG	2 cut(s)	273, 2023
BslFI	GGGAC	5 cut(s)	59, 614, 728, 1589, 2274
BslI	CCNNNNNNNGG	4 cut(s)	610, 766, 2298, 2374
BsmAI	GTCTC	4 cut(s)	264, 1322, 1394, 2061
BsmBI	CGTCTC	1 cut(s)	1394
BsmFI	GGGAC	5 cut(s)	59, 614, 728, 1589, 2274
BsmI	GAATGC	3 cut(s)	46, 543, 1442
BsnI	GGCC	6 cut(s)	141, 824, 1253, 2010, 2367, 2396
Bsp1286I	GDGCHC	2 cut(s)	2064, 2160
Bsp1407I	TGTACA	1 cut(s)	408
Bsp143I	GATC	3 cut(s)	1320, 1818, 2191
Bsp1720I	GCTNAGC	2 cut(s)	445, 1583
BspACI	CCGC	5 cut(s)	366, 646, 806, 1275, 1800
BspANI	GGCC	6 cut(s)	141, 824, 1253, 2010, 2367, 2396
BspCNI	CTCAG	5 cut(s)	83, 906, 1342, 1563, 2089
BspFNI	CGCG	2 cut(s)	929, 1275
BspLI	GGNNCC	3 cut(s)	602, 2016, 2290
BspMAI	CTGCAG	1 cut(s)	2444
BspPI	GGATC	2 cut(s)	1315, 2199
BspT107I	GGYRCC	1 cut(s)	2288
BsrGI	TGTACA	1 cut(s)	408
BsrI	ACTGG	3 cut(s)	1041, 1174, 1699
BssECI	CCNNGG	1 cut(s)	271
BssMI	GATC	3 cut(s)	1320, 1818, 2191
BssNAI	GTATAC	1 cut(s)	1926
Bst1107I	GTATAC	1 cut(s)	1926
Bst2UI	CCWGG	3 cut(s)	605, 657, 2012
Bst4CI	ACNGT	6 cut(s)	413, 1645, 1758, 2051, 2267, 2313
Bst6I	CTCTTC	4 cut(s)	292, 908, 939, 1464
BstAUI	TGTACA	1 cut(s)	408
BstBAI	YACGTR	3 cut(s)	564, 774, 2241
BstDEI	CTNAG	9 cut(s)	70, 381, 445, 801, 893, 1350, 1571, 1583, 2097
BstEII	GGTNACC	1 cut(s)	754
BstENI	CCTNNNNNAGG	1 cut(s)	2372
BstF5I	GGATG	8 cut(s)	168, 654, 849, 856, 1027, 1410, 1933, 2136
BstFNI	CGCG	2 cut(s)	929, 1275
BstH2I	RGCGCY	1 cut(s)	2424
BstHHI	GCGC	1 cut(s)	2423
BstKTI	GATC	3 cut(s)	1323, 1821, 2194
BstMAI	GTCTC	4 cut(s)	264, 1322, 1394, 2061
BstMBI	GATC	3 cut(s)	1320, 1818, 2191
BstMWI	GCNNNNNNNGC	4 cut(s)	581, 1250, 1806, 2164
BstNI	CCWGG	3 cut(s)	605, 657, 2012
BstNSI	RCATGY	2 cut(s)	358, 1582
BstPI	GGTNACC	1 cut(s)	754
BstSCI	CCNGG	5 cut(s)	271, 603, 655, 2010, 2022
BstSFI	CTRYAG	2 cut(s)	2000, 2440
BstSLI	GKGCMC	1 cut(s)	2160
BstSNI	TACGTA	2 cut(s)	774, 2241
BstUI	CGCG	2 cut(s)	929, 1275
BstV1I	GCAGC	1 cut(s)	2454
BstV2I	GAAGAC	1 cut(s)	1779
BstX2I	RGATCY	2 cut(s)	1320, 2191
BstYI	RGATCY	2 cut(s)	1320, 2191
BstZ17I	GTATAC	1 cut(s)	1926
BsuRI	GGCC	6 cut(s)	141, 824, 1253, 2010, 2367, 2396
BtrI	CACGTC	2 cut(s)	11, 2346
BtsCI	GGATG	8 cut(s)	168, 654, 849, 856, 1027, 1410, 1933, 2136
BtsIMutI	CAGTG	6 cut(s)	294, 901, 1034, 1216, 2263, 2290
CaiI	CAGNNNCTG	1 cut(s)	104
CfoI	GCGC	1 cut(s)	2423
Cfr13I	GGNCC	3 cut(s)	140, 601, 2008
CseI	GACGC	2 cut(s)	725, 935
Csp6I	GTAC	7 cut(s)	409, 432, 775, 1271, 1568, 1641, 2302
CviAII	CATG	8 cut(s)	188, 334, 355, 1579, 1736, 1762, 1909, 2351
CviQI	GTAC	7 cut(s)	409, 432, 775, 1271, 1568, 1641, 2302
DdeI	CTNAG	9 cut(s)	70, 381, 445, 801, 893, 1350, 1571, 1583, 2097
DpnI	GATC	3 cut(s)	1322, 1820, 2193
DpnII	GATC	3 cut(s)	1320, 1818, 2191
EaeI	YGGCCR	1 cut(s)	1251
Eam1104I	CTCTTC	4 cut(s)	292, 908, 939, 1464
EarI	CTCTTC	4 cut(s)	292, 908, 939, 1464
Eco105I	TACGTA	2 cut(s)	774, 2241
Eco147I	AGGCCT	1 cut(s)	824
Eco47I	GGWCC	1 cut(s)	601
Eco57I	CTGAAG	4 cut(s)	414, 1791, 2266, 2294
Eco91I	GGTNACC	1 cut(s)	754
EcoNI	CCTNNNNNAGG	1 cut(s)	2372
EcoO109I	RGGNCCY	1 cut(s)	601
EcoO65I	GGTNACC	1 cut(s)	754
EcoRI	GAATTC	1 cut(s)	628
EcoRII	CCWGG	3 cut(s)	603, 655, 2010
Esp3I	CGTCTC	1 cut(s)	1394
FaeI	CATG	8 cut(s)	191, 337, 358, 1582, 1739, 1765, 1912, 2354
FaqI	GGGAC	5 cut(s)	59, 614, 728, 1589, 2274
FatI	CATG	8 cut(s)	187, 333, 354, 1578, 1735, 1761, 1908, 2350
FauI	CCCGC	2 cut(s)	799, 1268
FbaI	TGATCA	1 cut(s)	1818
FblI	GTMKAC	1 cut(s)	1925
Fnu4HI	GCNGC	2 cut(s)	366, 2443
FokI	GGATG	8 cut(s)	155, 661, 856, 863, 1034, 1397, 1920, 2143
Fsp4HI	GCNGC	2 cut(s)	366, 2443
FspBI	CTAG	2 cut(s)	623, 2479
GlaI	GCGC	1 cut(s)	2422
GluI	GCNGC	2 cut(s)	366, 2443
GsuI	CTGGAG	1 cut(s)	203
HaeII	RGCGCY	1 cut(s)	2424
HaeIII	GGCC	6 cut(s)	141, 824, 1253, 2010, 2367, 2396
HapII	CCGG	2 cut(s)	273, 2023
HgaI	GACGC	2 cut(s)	725, 935
HhaI	GCGC	1 cut(s)	2423
Hin1II	CATG	8 cut(s)	191, 337, 358, 1582, 1739, 1765, 1912, 2354
Hin6I	GCGC	1 cut(s)	2421
HinP1I	GCGC	1 cut(s)	2421
HincII	GTYRAC	1 cut(s)	2125
HindII	GTYRAC	1 cut(s)	2125
HindIII	AAGCTT	1 cut(s)	582
HpaII	CCGG	2 cut(s)	273, 2023
HphI	GGTGA	9 cut(s)	270, 490, 766, 821, 922, 1037, 1241, 1288, 1372
Hpy166II	GTNNAC	8 cut(s)	278, 434, 561, 899, 1926, 2107, 2125, 2270
Hpy188III	TCNNGA	6 cut(s)	1181, 1459, 1553, 1877, 1897, 2018
Hpy8I	GTNNAC	8 cut(s)	278, 434, 561, 899, 1926, 2107, 2125, 2270
Hpy99I	CGWCG	1 cut(s)	2347
HpyCH4III	ACNGT	6 cut(s)	413, 1645, 1758, 2051, 2267, 2313
HpyCH4IV	ACGT	7 cut(s)	10, 563, 773, 1403, 2240, 2345, 2475
HpyF10VI	GCNNNNNNNGC	4 cut(s)	581, 1250, 1806, 2164
HpyF3I	CTNAG	9 cut(s)	70, 381, 445, 801, 893, 1350, 1571, 1583, 2097
HpySE526I	ACGT	7 cut(s)	10, 563, 773, 1403, 2240, 2345, 2475
Hsp92II	CATG	8 cut(s)	191, 337, 358, 1582, 1739, 1765, 1912, 2354
HspAI	GCGC	1 cut(s)	2421
Ksp22I	TGATCA	1 cut(s)	1818
Kzo9I	GATC	3 cut(s)	1320, 1818, 2191
Lsp1109I	GCAGC	1 cut(s)	2454
LweI	GCATC	5 cut(s)	375, 453, 1302, 1651, 1843
MaeI	CTAG	2 cut(s)	623, 2479
MaeII	ACGT	7 cut(s)	10, 563, 773, 1403, 2240, 2345, 2475
MaeIII	GTNAC	7 cut(s)	478, 754, 769, 874, 1032, 1714, 2051
MalI	GATC	3 cut(s)	1322, 1820, 2193
MboI	GATC	3 cut(s)	1320, 1818, 2191
MflI	RGATCY	2 cut(s)	1320, 2191
MhlI	GDGCHC	2 cut(s)	2064, 2160
MlsI	TGGCCA	1 cut(s)	1253
MluNI	TGGCCA	1 cut(s)	1253
MlyI	GAGTC	7 cut(s)	95, 219, 386, 539, 1734, 1909, 2218
MmeI	TCCRAC	2 cut(s)	795, 1730
Mox20I	TGGCCA	1 cut(s)	1253
MroXI	GAANNNNTTC	2 cut(s)	2176, 2279
MscI	TGGCCA	1 cut(s)	1253
MseI	TTAA	5 cut(s)	1077, 1280, 1419, 1491, 2256
Msp20I	TGGCCA	1 cut(s)	1253
MspI	CCGG	2 cut(s)	273, 2023
MspR9I	CCNGG	5 cut(s)	273, 605, 657, 2012, 2024
Mva1269I	GAATGC	3 cut(s)	46, 543, 1442
MvaI	CCWGG	3 cut(s)	605, 657, 2012
MvnI	CGCG	2 cut(s)	929, 1275
MwoI	GCNNNNNNNGC	4 cut(s)	581, 1250, 1806, 2164
NciI	CCSGG	2 cut(s)	273, 2024
NdeII	GATC	3 cut(s)	1320, 1818, 2191
NlaIII	CATG	8 cut(s)	191, 337, 358, 1582, 1739, 1765, 1912, 2354
NlaIV	GGNNCC	3 cut(s)	602, 2016, 2290
NmeAIII	GCCGAG	1 cut(s)	2422
NmuCI	GTSAC	4 cut(s)	478, 754, 874, 1032
NspI	RCATGY	2 cut(s)	358, 1582
PceI	AGGCCT	1 cut(s)	824
PcsI	WCGNNNNNNNCGW	1 cut(s)	779
PctI	GAATGC	3 cut(s)	46, 543, 1442
PdmI	GAANNNNTTC	2 cut(s)	2176, 2279
PfeI	GAWTC	6 cut(s)	470, 652, 918, 1651, 1671, 2149
PflFI	GACNNNGTC	1 cut(s)	903
PkrI	GCNGC	2 cut(s)	367, 2444
PleI	GAGTC	7 cut(s)	95, 218, 385, 538, 1734, 1908, 2218
PpsI	GAGTC	7 cut(s)	95, 218, 385, 538, 1734, 1908, 2218
Ppu21I	YACGTR	3 cut(s)	564, 774, 2241
PpuMI	RGGWCCY	1 cut(s)	601
PshBI	ATTAAT	2 cut(s)	1077, 1419
Psp5II	RGGWCCY	1 cut(s)	601
Psp6I	CCWGG	3 cut(s)	603, 655, 2010
PspEI	GGTNACC	1 cut(s)	754
PspGI	CCWGG	3 cut(s)	603, 655, 2010
PspN4I	GGNNCC	3 cut(s)	602, 2016, 2290
PspPI	GGNCC	3 cut(s)	140, 601, 2008
PspPPI	RGGWCCY	1 cut(s)	601
PstI	CTGCAG	1 cut(s)	2444
PstNI	CAGNNNCTG	1 cut(s)	104
PsuI	RGATCY	2 cut(s)	1320, 2191
PsyI	GACNNNGTC	1 cut(s)	903
RsaI	GTAC	7 cut(s)	410, 433, 776, 1272, 1569, 1642, 2303
RsaNI	GTAC	7 cut(s)	409, 432, 775, 1271, 1568, 1641, 2302
SaqAI	TTAA	5 cut(s)	1077, 1280, 1419, 1491, 2256
SatI	GCNGC	2 cut(s)	366, 2443
Sau3AI	GATC	3 cut(s)	1320, 1818, 2191
Sau96I	GGNCC	3 cut(s)	140, 601, 2008
ScaI	AGTACT	1 cut(s)	1569
SchI	GAGTC	7 cut(s)	95, 219, 386, 539, 1734, 1909, 2218
ScrFI	CCNGG	5 cut(s)	273, 605, 657, 2012, 2024
SduI	GDGCHC	2 cut(s)	2064, 2160
SfaNI	GCATC	5 cut(s)	375, 453, 1302, 1651, 1843
SfcI	CTRYAG	2 cut(s)	2000, 2440
SinI	GGWCC	1 cut(s)	601
SmlI	CTYRAG	1 cut(s)	2214
SmoI	CTYRAG	1 cut(s)	2214
SnaBI	TACGTA	2 cut(s)	774, 2241
SseBI	AGGCCT	1 cut(s)	824
SsiI	CCGC	5 cut(s)	366, 646, 806, 1275, 1800
SspI	AATATT	1 cut(s)	1501
SspMI	CTAG	2 cut(s)	623, 2479
StuI	AGGCCT	1 cut(s)	824
StyD4I	CCNGG	5 cut(s)	271, 603, 655, 2010, 2022
TaaI	ACNGT	6 cut(s)	413, 1645, 1758, 2051, 2267, 2313
TaiI	ACGT	7 cut(s)	13, 566, 776, 1406, 2243, 2348, 2478
TaqI	TCGA	7 cut(s)	528, 547, 921, 1222, 1446, 1677, 2342
TatI	WGTACW	2 cut(s)	408, 1567
TauI	GCSGC	1 cut(s)	368
TfiI	GAWTC	6 cut(s)	470, 652, 918, 1651, 1671, 2149
Tru1I	TTAA	5 cut(s)	1077, 1280, 1419, 1491, 2256
Tru9I	TTAA	5 cut(s)	1077, 1280, 1419, 1491, 2256
TscAI	CASTG	6 cut(s)	301, 901, 1041, 1216, 2270, 2290
TseFI	GTSAC	4 cut(s)	478, 754, 874, 1032
TseI	GCWGC	1 cut(s)	2442
Tsp45I	GTSAC	4 cut(s)	478, 754, 874, 1032
TspGWI	ACGGA	3 cut(s)	259, 1313, 2079
TspRI	CASTG	6 cut(s)	301, 901, 1041, 1216, 2270, 2290
Tth111I	GACNNNGTC	1 cut(s)	903
VpaK11BI	GGWCC	1 cut(s)	601
VspI	ATTAAT	2 cut(s)	1077, 1419
XagI	CCTNNNNNAGG	1 cut(s)	2372
XapI	RAATTY	4 cut(s)	628, 763, 1393, 2175
XceI	RCATGY	2 cut(s)	358, 1582
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1261
XmiI	GTMKAC	1 cut(s)	1925
XmnI	GAANNNNTTC	2 cut(s)	2176, 2279
XspI	CTAG	2 cut(s)	623, 2479
ZrmI	AGTACT	1 cut(s)	1569

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.