MD12G1036300.v1.1

Syntaxin 6, N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004239
Physical Location & Seq
Forward (+)
3908404 .. 3912155
3752 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1036300.v1.1.491

Sequence Viewer

Length: 1158 bp
ATGGCGTCGAGCTTGCACCAATGGGAGTCGGACCCTCTGTATTCCGCGGCCGAAGTCGTCCAAGATTCCGCTGACAGGATGGAATCGGTTTTCCGCTCTCTGCTGCATGAGCTCAGTCTTGTTCAAGGTGACTGTCCAGACCCAAAGTTACATGTGTCAATAGATTATCACAAACGCGATCTTGCTACTACACTTGAAACGACAAAGTGGCAGTTGGAAGACTTTGAAAGAGCAGTGGCAGGCAGGTCTCAAAATCGGGAGGATGTAAATTCAAGACACAAGCAGTTTATCGGAGCCATGAGGGAACAAATACTTTATGTGGAGAAGAGCTTGGAGGGCACACCGATAGGAGATCCCATGCGAAATACAGAGTGGGTTAACTTGAATGAACAAGATAGACATGGTTTGGCATTGTTTCTTTCTGGTGGAAATAACGCCGAGCCCACTGATGGTCAAGAAGTGGAGGACAGCAGTATCTTGAGAAGATTTCTTGATCCAACCGCATCTAGTGCTAAAGACAGTACTTCTGGTATAGATGAGCACAAAAACAGAGAAATTGAGGACTTGAATACGAATGGAGTGGTGCATGTGGACCGTGTTATTGAATCTAGAAAGGAGAACAACTTGAGTAAAGTTGGTTACTATAACAGATTTGGTGAAGGTGGGAGTTGGGACATCGGCAATACAAGGTTGAAATCTGATTTTACAGATTCTTTTCCACAGACCTCTTGTAACAGATATGGAGGTGGTGAGAGTCGGGATCTGGAAGCTAATGAAGCCAAACCTGAAAGCTTCAGCAGAACAAACATATTGGGGTTCTTGAATACTGCATGGAGTGTGTATGGGAGTAGGGTCACTGGGAGCTATACAAAGAGATTTAAAGATGGAGAAGAACAAAGTCATTCCCCATCAATTGCTGATGTTTCTCATGCCGCTCAGGTTCAGCATCAGGGAACGTGGTTGTATAGAAATTTTAAAGAGTTGTGTATGGGGATCCGGACAATAGTTATGTACTTACGGACCTGGCTTGGTGTATTTAGGGCAAGATATGAAAGATCACCTTATCACATTCAAGTTCAGCGGCATTCTATTCACGTGGTATTGATAATACTACTTTCGCTCATTATTTTAGGCACACTGGTGTCCCGGCTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syntaxin-6_N

Protein Analysis

386

Amino Acids

43.68

Weight (kDa)

5.81

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
STX6_10_61_N PF09177 11 - 105 1.4e-13 Syntaxin 6/10/61, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 234
AccBSI CCGCTC 2 cut(s) 96, 935
AccII CGCG 2 cut(s) 47, 177
AccIII TCCGGA 1 cut(s) 996
AciI CCGC 7 cut(s) 45, 47, 69, 94, 501, 933, 1081
AclWI GGATC 5 cut(s) 347, 488, 768, 988, 1001
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 2 cut(s) 268, 970
AcuI CTGAAG 1 cut(s) 778
AcvI CACGTG 1 cut(s) 1096
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 523, 1013
AfiI CCNNNNNNNGG 2 cut(s) 75, 449
AflIII ACRYGT 1 cut(s) 151
AjnI CCWGG 1 cut(s) 1022
AleI CACNNNNGTG 1 cut(s) 1139
AluBI AGCT 6 cut(s) 12, 112, 330, 770, 792, 864
AluI AGCT 6 cut(s) 12, 112, 330, 770, 792, 864
Alw21I GWGCWC 2 cut(s) 114, 543
Alw26I GTCTC 1 cut(s) 252
AlwI GGATC 5 cut(s) 347, 488, 768, 988, 1001
Aor13HI TCCGGA 1 cut(s) 996
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 1 cut(s) 103
ApoI RAATTY 2 cut(s) 268, 970
AspS9I GGNCC 3 cut(s) 31, 592, 1020
AsuC2I CCSGG 1 cut(s) 1147
AsuHPI GGTGA 4 cut(s) 140, 668, 761, 1050
AvaII GGWCC 3 cut(s) 31, 592, 1020
BaeGI GKGCMC 1 cut(s) 341
BaeI ACNNNNGTAYC 2 cut(s) 457, 490
BamHI GGATCC 1 cut(s) 993
BanII GRGCYC 2 cut(s) 114, 444
BbrPI CACGTG 1 cut(s) 1096
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 2 cut(s) 114, 543
BbvI GCAGC 1 cut(s) 90
BccI CCATC 4 cut(s) 73, 443, 878, 916
BciT130I CCWGG 1 cut(s) 1024
BcnI CCSGG 1 cut(s) 1147
BcoDI GTCTC 1 cut(s) 252
BfaI CTAG 2 cut(s) 507, 609
BfuAI ACCTGC 1 cut(s) 234
BisI GCNGC 4 cut(s) 48, 104, 933, 1082
BlsI GCNGC 4 cut(s) 49, 105, 934, 1083
BmcAI AGTACT 1 cut(s) 523
Bme1390I CCNGG 2 cut(s) 1024, 1147
Bme18I GGWCC 3 cut(s) 31, 592, 1020
BmgT120I GGNCC 3 cut(s) 31, 592, 1020
BmiI GGNNCC 3 cut(s) 33, 295, 995
BmrFI CCNGG 2 cut(s) 1024, 1147
BmrI ACTGGG 1 cut(s) 867
BmsI GCATC 2 cut(s) 512, 955
BmuI ACTGGG 1 cut(s) 867
BpiI GAAGAC 1 cut(s) 225
Bpu10I CCTNAGC 1 cut(s) 936
BpuEI CTTGAG 2 cut(s) 499, 646
BpuMI CCSGG 1 cut(s) 1147
BsaAI YACGTR 1 cut(s) 1096
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 45
BsaWI WCCGGW 1 cut(s) 996
Bsc4I CCNNNNNNNGG 2 cut(s) 75, 449
Bse1I ACTGG 2 cut(s) 862, 1143
BseAI TCCGGA 1 cut(s) 996
BseBI CCWGG 1 cut(s) 1024
BseDI CCNNGG 1 cut(s) 45
BseGI GGATG 2 cut(s) 84, 268
BseLI CCNNNNNNNGG 2 cut(s) 75, 449
BseMII CTCAG 2 cut(s) 127, 950
BseNI ACTGG 2 cut(s) 862, 1143
BseSI GKGCMC 1 cut(s) 341
BseX3I CGGCCG 1 cut(s) 48
BseXI GCAGC 1 cut(s) 90
Bsh1236I CGCG 2 cut(s) 47, 177
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 1 cut(s) 50
BsiEI CGRYCG 1 cut(s) 51
BsiHKAI GWGCWC 2 cut(s) 114, 543
BsiSI CCGG 2 cut(s) 997, 1147
BslFI GGGAC 2 cut(s) 686, 1129
BslI CCNNNNNNNGG 2 cut(s) 75, 449
BsmAI GTCTC 1 cut(s) 252
BsmFI GGGAC 2 cut(s) 686, 1129
BsmI GAATGC 1 cut(s) 1084
BsnI GGCC 1 cut(s) 50
Bso31I GGTCTC 1 cut(s) 252
Bsp1286I GDGCHC 4 cut(s) 114, 341, 444, 543
Bsp13I TCCGGA 1 cut(s) 996
Bsp143I GATC 6 cut(s) 178, 352, 493, 760, 993, 1055
BspACI CCGC 7 cut(s) 45, 47, 69, 94, 501, 933, 1081
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 2 cut(s) 126, 949
BspEI TCCGGA 1 cut(s) 996
BspFNI CGCG 2 cut(s) 47, 177
BspLI GGNNCC 3 cut(s) 33, 295, 995
BspMI ACCTGC 1 cut(s) 234
BspPI GGATC 5 cut(s) 347, 488, 768, 988, 1001
BspQI GCTCTTC 1 cut(s) 320
BspTNI GGTCTC 1 cut(s) 252
BsrBI CCGCTC 2 cut(s) 96, 935
BsrI ACTGG 2 cut(s) 862, 1143
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 6 cut(s) 178, 352, 493, 760, 993, 1055
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 1 cut(s) 1024
Bst4CI ACNGT 3 cut(s) 134, 521, 596
Bst6I CTCTTC 1 cut(s) 320
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 509
BstBAI YACGTR 1 cut(s) 1096
BstC8I GCNNGC 3 cut(s) 14, 241, 1152
BstDEI CTNAG 2 cut(s) 113, 936
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 2 cut(s) 84, 268
BstFNI CGCG 2 cut(s) 47, 177
BstKTI GATC 6 cut(s) 181, 355, 496, 763, 996, 1058
BstMAI GTCTC 1 cut(s) 252
BstMBI GATC 6 cut(s) 178, 352, 493, 760, 993, 1055
BstMCI CGRYCG 1 cut(s) 51
BstMWI GCNNNNNNNGC 4 cut(s) 109, 336, 509, 776
BstNI CCWGG 1 cut(s) 1024
BstNSI RCATGY 2 cut(s) 155, 590
BstSCI CCNGG 2 cut(s) 1022, 1145
BstSLI GKGCMC 1 cut(s) 341
BstUI CGCG 2 cut(s) 47, 177
BstV1I GCAGC 1 cut(s) 90
BstV2I GAAGAC 1 cut(s) 225
BstX2I RGATCY 3 cut(s) 352, 760, 993
BstYI RGATCY 3 cut(s) 352, 760, 993
BstZI CGGCCG 1 cut(s) 48
BsuRI GGCC 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 2 cut(s) 84, 268
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 4 cut(s) 240, 444, 855, 1136
BveI ACCTGC 1 cut(s) 234
Cac8I GCNNGC 3 cut(s) 14, 241, 1152
Cfr13I GGNCC 3 cut(s) 31, 592, 1020
Cfr42I CCGCGG 1 cut(s) 48
Csp6I GTAC 2 cut(s) 522, 1012
CviAII CATG 8 cut(s) 107, 152, 298, 358, 401, 587, 831, 929
CviQI GTAC 2 cut(s) 522, 1012
DdeI CTNAG 2 cut(s) 113, 936
DpnI GATC 6 cut(s) 180, 354, 495, 762, 995, 1057
DpnII GATC 6 cut(s) 178, 352, 493, 760, 993, 1055
DraI TTTAAA 2 cut(s) 880, 976
EaeI YGGCCR 1 cut(s) 48
EagI CGGCCG 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 320
EarI CTCTTC 1 cut(s) 320
Ecl136II GAGCTC 1 cut(s) 112
EclXI CGGCCG 1 cut(s) 48
Eco24I GRGCYC 2 cut(s) 114, 444
Eco31I GGTCTC 1 cut(s) 252
Eco47I GGWCC 3 cut(s) 31, 592, 1020
Eco52I CGGCCG 1 cut(s) 48
Eco53kI GAGCTC 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 778
Eco72I CACGTG 1 cut(s) 1096
EcoICRI GAGCTC 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 1022
EcoT38I GRGCYC 2 cut(s) 114, 444
FaeI CATG 8 cut(s) 110, 155, 301, 361, 404, 590, 834, 932
FalI AAGNNNNNCTT 2 cut(s) 1045, 1077
FaqI GGGAC 2 cut(s) 686, 1129
FatI CATG 8 cut(s) 106, 151, 297, 357, 400, 586, 830, 928
Fnu4HI GCNGC 4 cut(s) 48, 104, 933, 1082
FokI GGATG 2 cut(s) 91, 275
FriOI GRGCYC 2 cut(s) 114, 444
Fsp4HI GCNGC 4 cut(s) 48, 104, 933, 1082
FspBI CTAG 2 cut(s) 507, 609
GluI GCNGC 4 cut(s) 48, 104, 933, 1082
HaeIII GGCC 1 cut(s) 50
HapII CCGG 2 cut(s) 997, 1147
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 8 cut(s) 110, 155, 301, 361, 404, 590, 834, 932
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HindIII AAGCTT 1 cut(s) 790
HinfI GANTC 6 cut(s) 26, 65, 83, 605, 710, 754
HpaI GTTAAC 1 cut(s) 379
HpaII CCGG 2 cut(s) 997, 1147
HphI GGTGA 4 cut(s) 140, 668, 761, 1050
Hpy166II GTNNAC 2 cut(s) 379, 592
Hpy188I TCNGA 3 cut(s) 31, 293, 700
Hpy8I GTNNAC 2 cut(s) 379, 592
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 653
HpyCH4III ACNGT 3 cut(s) 134, 521, 596
HpyCH4IV ACGT 2 cut(s) 956, 1095
HpyCH4V TGCA 4 cut(s) 16, 106, 586, 830
HpyF10VI GCNNNNNNNGC 4 cut(s) 109, 336, 509, 776
HpyF3I CTNAG 2 cut(s) 113, 936
HpySE526I ACGT 2 cut(s) 956, 1095
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 8 cut(s) 110, 155, 301, 361, 404, 590, 834, 932
Kpn2I TCCGGA 1 cut(s) 996
KspAI GTTAAC 1 cut(s) 379
KspI CCGCGG 1 cut(s) 48
Kzo9I GATC 6 cut(s) 178, 352, 493, 760, 993, 1055
LguI GCTCTTC 1 cut(s) 320
LmnI GCTCC 2 cut(s) 293, 861
Lsp1109I GCAGC 1 cut(s) 90
LweI GCATC 2 cut(s) 512, 955
MaeI CTAG 2 cut(s) 507, 609
MaeII ACGT 2 cut(s) 956, 1095
MaeIII GTNAC 5 cut(s) 128, 147, 638, 731, 853
MalI GATC 6 cut(s) 180, 354, 495, 762, 995, 1057
MbiI CCGCTC 2 cut(s) 96, 935
MboI GATC 6 cut(s) 178, 352, 493, 760, 993, 1055
MboII GAAGA 4 cut(s) 230, 337, 495, 902
MfeI CAATTG 1 cut(s) 912
MflI RGATCY 3 cut(s) 352, 760, 993
MhlI GDGCHC 4 cut(s) 114, 341, 444, 543
MluCI AATT 4 cut(s) 268, 555, 912, 970
MlyI GAGTC 2 cut(s) 35, 763
MmeI TCCRAC 3 cut(s) 9, 195, 521
MnlI CCTC 8 cut(s) 45, 253, 294, 328, 457, 553, 736, 737
MroI TCCGGA 1 cut(s) 996
MseI TTAA 4 cut(s) 378, 879, 975, 1156
MslI CAYNNNNRTG 1 cut(s) 1139
MspA1I CMGCKG 3 cut(s) 47, 71, 1081
MspI CCGG 2 cut(s) 997, 1147
MspR9I CCNGG 2 cut(s) 1024, 1147
MunI CAATTG 1 cut(s) 912
Mva1269I GAATGC 1 cut(s) 1084
MvaI CCWGG 1 cut(s) 1024
MvnI CGCG 2 cut(s) 47, 177
MwoI GCNNNNNNNGC 4 cut(s) 109, 336, 509, 776
NciI CCSGG 1 cut(s) 1147
NdeII GATC 6 cut(s) 178, 352, 493, 760, 993, 1055
NlaIII CATG 8 cut(s) 110, 155, 301, 361, 404, 590, 834, 932
NlaIV GGNNCC 3 cut(s) 33, 295, 995
NmeAIII GCCGAG 1 cut(s) 463
NmuCI GTSAC 2 cut(s) 128, 853
NspI RCATGY 2 cut(s) 155, 590
OliI CACNNNNGTG 1 cut(s) 1139
PciI ACATGT 1 cut(s) 151
PciSI GCTCTTC 1 cut(s) 320
PctI GAATGC 1 cut(s) 1084
PfeI GAWTC 4 cut(s) 65, 83, 605, 710
PkrI GCNGC 4 cut(s) 49, 105, 934, 1083
PleI GAGTC 2 cut(s) 34, 762
PmaCI CACGTG 1 cut(s) 1096
PmlI CACGTG 1 cut(s) 1096
PpsI GAGTC 2 cut(s) 34, 762
Ppu21I YACGTR 1 cut(s) 1096
PscI ACATGT 1 cut(s) 151
Psp124BI GAGCTC 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 1022
PspCI CACGTG 1 cut(s) 1096
PspGI CCWGG 1 cut(s) 1022
PspN4I GGNNCC 3 cut(s) 33, 295, 995
PspPI GGNCC 3 cut(s) 31, 592, 1020
PsuI RGATCY 3 cut(s) 352, 760, 993
RsaI GTAC 2 cut(s) 523, 1013
RsaNI GTAC 2 cut(s) 522, 1012
RseI CAYNNNNRTG 1 cut(s) 1139
SacI GAGCTC 1 cut(s) 114
SacII CCGCGG 1 cut(s) 48
SapI GCTCTTC 1 cut(s) 320
SaqAI TTAA 4 cut(s) 378, 879, 975, 1156
SatI GCNGC 4 cut(s) 48, 104, 933, 1082
Sau3AI GATC 6 cut(s) 178, 352, 493, 760, 993, 1055
Sau96I GGNCC 3 cut(s) 31, 592, 1020
ScaI AGTACT 1 cut(s) 523
SchI GAGTC 2 cut(s) 35, 763
ScrFI CCNGG 2 cut(s) 1024, 1147
SduI GDGCHC 4 cut(s) 114, 341, 444, 543
SfaNI GCATC 2 cut(s) 512, 955
Sfr303I CCGCGG 1 cut(s) 48
SgrBI CCGCGG 1 cut(s) 48
SinI GGWCC 3 cut(s) 31, 592, 1020
SmiMI CAYNNNNRTG 1 cut(s) 1139
SmlI CTYRAG 2 cut(s) 478, 625
SmoI CTYRAG 2 cut(s) 478, 625
Sse9I AATT 4 cut(s) 268, 555, 912, 970
SsiI CCGC 7 cut(s) 45, 47, 69, 94, 501, 933, 1081
SspMI CTAG 2 cut(s) 507, 609
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 2 cut(s) 1022, 1145
TaaI ACNGT 3 cut(s) 134, 521, 596
TaiI ACGT 2 cut(s) 959, 1098
TaqI TCGA 1 cut(s) 8
TasI AATT 4 cut(s) 268, 555, 912, 970
TatI WGTACW 2 cut(s) 521, 1011
TauI GCSGC 3 cut(s) 50, 935, 1084
TfiI GAWTC 4 cut(s) 65, 83, 605, 710
Tru1I TTAA 4 cut(s) 378, 879, 975, 1156
Tru9I TTAA 4 cut(s) 378, 879, 975, 1156
TscAI CASTG 4 cut(s) 240, 451, 862, 1143
TseFI GTSAC 2 cut(s) 128, 853
TseI GCWGC 1 cut(s) 103
Tsp45I GTSAC 2 cut(s) 128, 853
TspDTI ATGAA 3 cut(s) 402, 789, 1065
TspGWI ACGGA 1 cut(s) 1033
TspRI CASTG 4 cut(s) 240, 451, 862, 1143
VpaK11BI GGWCC 3 cut(s) 31, 592, 1020
XapI RAATTY 2 cut(s) 268, 970
XbaI TCTAGA 1 cut(s) 608
XceI RCATGY 2 cut(s) 155, 590
XspI CTAG 2 cut(s) 507, 609
ZrmI AGTACT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.