MD14G1023100.v1.1

Syntaxin 6, N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004239
Physical Location & Seq
Forward (+)
2071288 .. 2074808
3521 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1023100.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGGCGTCGAGCTTGCACCAATGGGAGTCGGACCCTCTGTTCTCCGCAGCCGAAGTCGTCCAAGATTCCGCTGACAGGATGGAATCGGTTTTCCGCTCTCTGCTGCATGAGCTCAGTCTTGTTCAAGGTGACTGTCCAGACCCAAAGTTACGTGTGTCAATAGATTATCACAAACGTGATCTTGCTACAACACTTGAAACTGCAAAGTGGCAGTTGGAAGATTTTGAAAGAGCAGTAAGCTTTTCAGCTATGGCAGGAAGGTCTCAAAATTGGGAGGATGTAATTTCAAGACACAAACAGTTTATAGGAGCCATTAGGGAACACATACTTTATGTGGAGAAGAGCTTGGAGGGTACAGCTATAGGAGATGATCTCATGAGAAATACAGAGTGGGTTAACTTGAATGAACAAGATAGAGATGGTTTGGCATTGTTCCTTTCTGGGGGAAATAATGCCGAGCCCACTGATTGTCAAAAAGTGGAAGACAACAGTATCTTGAGAAGATTTCTTGATCCAACCACATCTAATGCTAAAGACGGCACTTCTGGTATCGTTGAGGACAAAAACAGAGAAATTGAGGACTTGAATATGAATGGAGTGGCACATGTGGACCGTGTTATTGAATCTATCAAGGAGAATAACTTGAGGAAAGTTGGTTCTTATAGCAGATTCGGTGAAGGTGGGAGTTGGGACATTGGCAGTACAAGGTTGAAATCTGACTTTACAGATTCTTTTCCAGAGACCTCTTGTAACAGATATGGAGGTGGTGAGAGTTGGGATCTGGAAGCTAACGAAGCCAAACCTGAAAGCTTCAGCAGAACAAACGTATTTGGGTTCTTGAATACGGCATGGAGTGTTTATGGGAGTAGGGTAACTGGGAGCTATACAAAGAGATTTAAAGATGGAGAAGAACAAAGTCATTCCCCATCAAGTGCTGGTGTTTCTCATGGCGCACAGGGTCAGCATCAGGGACCGTGGTTGTATAGAAATTTCAAAGAGTTGGGTATGGGGATTCGGACAAAAGTAATGTACTTACGGACCTGGCTTGGTGTATTTAGGGCAAGATATGAAAGATCACCTTATCACATTCAAGTTCAGCGGCATTCTATTCAAGTGGTGTTGATAATACTACTTACGCTCATTATTTTAGGCATACTGGTGTCCCGGCTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syntaxin-6_N

Protein Analysis

392

Amino Acids

44.13

Weight (kDa)

5.64

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
STX6_10_61_N PF09177 11 - 109 3.5e-15 Syntaxin 6/10/61, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 96
AciI CCGC 4 cut(s) 45, 69, 94, 1099
AclWI GGATC 2 cut(s) 506, 786
AcsI RAATTY 1 cut(s) 988
AcuI CTGAAG 1 cut(s) 796
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 355, 703, 1031
AfiI CCNNNNNNNGG 2 cut(s) 75, 442
AflIII ACRYGT 2 cut(s) 151, 604
AjnI CCWGG 1 cut(s) 1040
AleI CACNNNNGTG 1 cut(s) 174
AloI GAACNNNNNNTCC 4 cut(s) 23, 55, 640, 672
AluBI AGCT 9 cut(s) 12, 112, 240, 248, 345, 359, 788, 810, 882
AluI AGCT 9 cut(s) 12, 112, 240, 248, 345, 359, 788, 810, 882
Alw21I GWGCWC 1 cut(s) 114
Alw26I GTCTC 2 cut(s) 267, 734
AlwI GGATC 2 cut(s) 506, 786
ApeKI GCWGC 2 cut(s) 47, 103
ApoI RAATTY 1 cut(s) 988
AspLEI GCGC 1 cut(s) 953
AspS9I GGNCC 4 cut(s) 31, 610, 971, 1038
AsuC2I CCSGG 1 cut(s) 1165
AsuHPI GGTGA 4 cut(s) 140, 686, 779, 1068
AvaII GGWCC 4 cut(s) 31, 610, 971, 1038
BaeI ACNNNNGTAYC 2 cut(s) 475, 508
BanII GRGCYC 2 cut(s) 114, 462
BbsI GAAGAC 1 cut(s) 489
Bbv12I GWGCWC 1 cut(s) 114
BbvI GCAGC 2 cut(s) 59, 90
BccI CCATC 4 cut(s) 73, 413, 896, 934
BceAI ACGGC 2 cut(s) 553, 861
BciT130I CCWGG 1 cut(s) 1042
BcnI CCSGG 1 cut(s) 1165
BcoDI GTCTC 2 cut(s) 267, 734
BfmI CTRYAG 1 cut(s) 360
BisI GCNGC 3 cut(s) 48, 104, 1100
BlsI GCNGC 3 cut(s) 49, 105, 1101
Bme1390I CCNGG 2 cut(s) 1042, 1165
Bme18I GGWCC 4 cut(s) 31, 610, 971, 1038
BmgT120I GGNCC 4 cut(s) 31, 610, 971, 1038
BmiI GGNNCC 3 cut(s) 33, 310, 972
BmrFI CCNGG 2 cut(s) 1042, 1165
BmrI ACTGGG 1 cut(s) 885
BmsI GCATC 1 cut(s) 973
BmuI ACTGGG 1 cut(s) 885
BpiI GAAGAC 1 cut(s) 489
BplI GAGNNNNNCTC 2 cut(s) 357, 389
BpuEI CTTGAG 2 cut(s) 517, 664
BpuMI CCSGG 1 cut(s) 1165
BsaAI YACGTR 1 cut(s) 152
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 267, 734
BsaJI CCNNGG 1 cut(s) 974
Bsc4I CCNNNNNNNGG 2 cut(s) 75, 442
Bse1I ACTGG 2 cut(s) 880, 1161
BseBI CCWGG 1 cut(s) 1042
BseDI CCNNGG 1 cut(s) 974
BseGI GGATG 2 cut(s) 84, 283
BseLI CCNNNNNNNGG 2 cut(s) 75, 442
BseMII CTCAG 1 cut(s) 127
BseNI ACTGG 2 cut(s) 880, 1161
BseXI GCAGC 2 cut(s) 59, 90
BsiHKAI GWGCWC 1 cut(s) 114
BsiSI CCGG 1 cut(s) 1165
BslFI GGGAC 3 cut(s) 704, 984, 1147
BslI CCNNNNNNNGG 2 cut(s) 75, 442
BsmAI GTCTC 2 cut(s) 267, 734
BsmFI GGGAC 3 cut(s) 704, 984, 1147
BsmI GAATGC 1 cut(s) 1102
Bso31I GGTCTC 2 cut(s) 267, 734
Bsp1286I GDGCHC 2 cut(s) 114, 462
Bsp143I GATC 5 cut(s) 178, 370, 511, 778, 1073
BspACI CCGC 4 cut(s) 45, 69, 94, 1099
BspCNI CTCAG 1 cut(s) 126
BspHI TCATGA 1 cut(s) 375
BspLI GGNNCC 3 cut(s) 33, 310, 972
BspPI GGATC 2 cut(s) 506, 786
BspQI GCTCTTC 1 cut(s) 335
BspTNI GGTCTC 2 cut(s) 267, 734
BsrBI CCGCTC 1 cut(s) 96
BsrI ACTGG 2 cut(s) 880, 1161
BssECI CCNNGG 1 cut(s) 974
BssMI GATC 5 cut(s) 178, 370, 511, 778, 1073
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 1 cut(s) 1042
Bst4CI ACNGT 5 cut(s) 134, 300, 491, 614, 975
Bst6I CTCTTC 1 cut(s) 335
BstACI GRCGYC 1 cut(s) 5
BstBAI YACGTR 1 cut(s) 152
BstC8I GCNNGC 2 cut(s) 14, 1170
BstDEI CTNAG 1 cut(s) 113
BstDSI CCRYGG 1 cut(s) 974
BstF5I GGATG 2 cut(s) 84, 283
BstHHI GCGC 1 cut(s) 953
BstKTI GATC 5 cut(s) 181, 373, 514, 781, 1076
BstMAI GTCTC 2 cut(s) 267, 734
BstMBI GATC 5 cut(s) 178, 370, 511, 778, 1073
BstMWI GCNNNNNNNGC 2 cut(s) 109, 794
BstNI CCWGG 1 cut(s) 1042
BstNSI RCATGY 1 cut(s) 608
BstSCI CCNGG 2 cut(s) 1040, 1163
BstSFI CTRYAG 1 cut(s) 360
BstV1I GCAGC 2 cut(s) 59, 90
BstV2I GAAGAC 1 cut(s) 489
BstX2I RGATCY 1 cut(s) 778
BstYI RGATCY 1 cut(s) 778
BtgI CCRYGG 1 cut(s) 974
BtsCI GGATG 2 cut(s) 84, 283
BtsIMutI CAGTG 1 cut(s) 462
Cac8I GCNNGC 2 cut(s) 14, 1170
CciI TCATGA 1 cut(s) 375
CfoI GCGC 1 cut(s) 953
Cfr13I GGNCC 4 cut(s) 31, 610, 971, 1038
Csp6I GTAC 3 cut(s) 354, 702, 1030
CviAII CATG 5 cut(s) 107, 376, 605, 849, 947
CviQI GTAC 3 cut(s) 354, 702, 1030
DdeI CTNAG 1 cut(s) 113
DpnI GATC 5 cut(s) 180, 372, 513, 780, 1075
DpnII GATC 5 cut(s) 178, 370, 511, 778, 1073
DraI TTTAAA 1 cut(s) 898
Eam1104I CTCTTC 1 cut(s) 335
EarI CTCTTC 1 cut(s) 335
Ecl136II GAGCTC 1 cut(s) 112
Eco24I GRGCYC 2 cut(s) 114, 462
Eco31I GGTCTC 2 cut(s) 267, 734
Eco47I GGWCC 4 cut(s) 31, 610, 971, 1038
Eco53kI GAGCTC 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 796
EcoICRI GAGCTC 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 1040
EcoT38I GRGCYC 2 cut(s) 114, 462
FaeI CATG 5 cut(s) 110, 379, 608, 852, 950
FalI AAGNNNNNCTT 2 cut(s) 1063, 1095
FaqI GGGAC 3 cut(s) 704, 984, 1147
FatI CATG 5 cut(s) 106, 375, 604, 848, 946
Fnu4HI GCNGC 3 cut(s) 48, 104, 1100
FokI GGATG 2 cut(s) 91, 290
FriOI GRGCYC 2 cut(s) 114, 462
Fsp4HI GCNGC 3 cut(s) 48, 104, 1100
GlaI GCGC 1 cut(s) 952
GluI GCNGC 3 cut(s) 48, 104, 1100
HapII CCGG 1 cut(s) 1165
HhaI GCGC 1 cut(s) 953
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 110, 379, 608, 852, 950
Hin6I GCGC 1 cut(s) 951
HinP1I GCGC 1 cut(s) 951
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HindIII AAGCTT 2 cut(s) 238, 808
HinfI GANTC 7 cut(s) 26, 65, 83, 623, 669, 728, 1012
HpaI GTTAAC 1 cut(s) 397
HpaII CCGG 1 cut(s) 1165
HphI GGTGA 4 cut(s) 140, 686, 779, 1068
Hpy166II GTNNAC 2 cut(s) 397, 610
Hpy188I TCNGA 3 cut(s) 31, 718, 1017
Hpy188III TCNNGA 8 cut(s) 137, 288, 376, 496, 509, 737, 782, 838
Hpy8I GTNNAC 2 cut(s) 397, 610
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 252, 671
HpyCH4III ACNGT 5 cut(s) 134, 300, 491, 614, 975
HpyCH4IV ACGT 3 cut(s) 151, 175, 825
HpyCH4V TGCA 3 cut(s) 16, 106, 203
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 794
HpyF3I CTNAG 1 cut(s) 113
HpySE526I ACGT 3 cut(s) 151, 175, 825
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 110, 379, 608, 852, 950
HspAI GCGC 1 cut(s) 951
KspAI GTTAAC 1 cut(s) 397
Kzo9I GATC 5 cut(s) 178, 370, 511, 778, 1073
LguI GCTCTTC 1 cut(s) 335
LmnI GCTCC 2 cut(s) 308, 879
Lsp1109I GCAGC 2 cut(s) 59, 90
LweI GCATC 1 cut(s) 973
MaeII ACGT 3 cut(s) 151, 175, 825
MaeIII GTNAC 4 cut(s) 128, 147, 749, 871
MalI GATC 5 cut(s) 180, 372, 513, 780, 1075
MbiI CCGCTC 1 cut(s) 96
MboI GATC 5 cut(s) 178, 370, 511, 778, 1073
MboII GAAGA 5 cut(s) 230, 352, 494, 513, 920
MflI RGATCY 1 cut(s) 778
MhlI GDGCHC 2 cut(s) 114, 462
MluCI AATT 4 cut(s) 268, 282, 573, 988
MlyI GAGTC 1 cut(s) 35
MmeI TCCRAC 3 cut(s) 9, 195, 539
MnlI CCTC 8 cut(s) 45, 268, 343, 550, 571, 639, 754, 755
MseI TTAA 3 cut(s) 396, 897, 1174
MslI CAYNNNNRTG 2 cut(s) 174, 1157
MspA1I CMGCKG 2 cut(s) 71, 1099
MspI CCGG 1 cut(s) 1165
MspR9I CCNGG 2 cut(s) 1042, 1165
Mva1269I GAATGC 1 cut(s) 1102
MvaI CCWGG 1 cut(s) 1042
MwoI GCNNNNNNNGC 2 cut(s) 109, 794
NciI CCSGG 1 cut(s) 1165
NdeII GATC 5 cut(s) 178, 370, 511, 778, 1073
NlaIII CATG 5 cut(s) 110, 379, 608, 852, 950
NlaIV GGNNCC 3 cut(s) 33, 310, 972
NmeAIII GCCGAG 1 cut(s) 481
NmuCI GTSAC 1 cut(s) 128
NspI RCATGY 1 cut(s) 608
OliI CACNNNNGTG 1 cut(s) 174
PagI TCATGA 1 cut(s) 375
PciI ACATGT 1 cut(s) 604
PciSI GCTCTTC 1 cut(s) 335
PctI GAATGC 1 cut(s) 1102
PfeI GAWTC 6 cut(s) 65, 83, 623, 669, 728, 1012
PkrI GCNGC 3 cut(s) 49, 105, 1101
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
Ppu21I YACGTR 1 cut(s) 152
PscI ACATGT 1 cut(s) 604
Psp124BI GAGCTC 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 1040
PspGI CCWGG 1 cut(s) 1040
PspN4I GGNNCC 3 cut(s) 33, 310, 972
PspPI GGNCC 4 cut(s) 31, 610, 971, 1038
PsuI RGATCY 1 cut(s) 778
RsaI GTAC 3 cut(s) 355, 703, 1031
RsaNI GTAC 3 cut(s) 354, 702, 1030
RseI CAYNNNNRTG 2 cut(s) 174, 1157
SacI GAGCTC 1 cut(s) 114
SapI GCTCTTC 1 cut(s) 335
SaqAI TTAA 3 cut(s) 396, 897, 1174
SatI GCNGC 3 cut(s) 48, 104, 1100
Sau3AI GATC 5 cut(s) 178, 370, 511, 778, 1073
Sau96I GGNCC 4 cut(s) 31, 610, 971, 1038
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 2 cut(s) 1042, 1165
SduI GDGCHC 2 cut(s) 114, 462
SfaNI GCATC 1 cut(s) 973
SfcI CTRYAG 1 cut(s) 360
SinI GGWCC 4 cut(s) 31, 610, 971, 1038
SmiMI CAYNNNNRTG 2 cut(s) 174, 1157
SmlI CTYRAG 2 cut(s) 496, 643
SmoI CTYRAG 2 cut(s) 496, 643
Sse9I AATT 4 cut(s) 268, 282, 573, 988
SsiI CCGC 4 cut(s) 45, 69, 94, 1099
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 2 cut(s) 1040, 1163
TaaI ACNGT 5 cut(s) 134, 300, 491, 614, 975
TaiI ACGT 3 cut(s) 154, 178, 828
TaqI TCGA 1 cut(s) 8
TasI AATT 4 cut(s) 268, 282, 573, 988
TatI WGTACW 2 cut(s) 701, 1029
TauI GCSGC 1 cut(s) 1102
TfiI GAWTC 6 cut(s) 65, 83, 623, 669, 728, 1012
Tru1I TTAA 3 cut(s) 396, 897, 1174
Tru9I TTAA 3 cut(s) 396, 897, 1174
TscAI CASTG 1 cut(s) 469
TseFI GTSAC 1 cut(s) 128
TseI GCWGC 2 cut(s) 47, 103
Tsp45I GTSAC 1 cut(s) 128
TspDTI ATGAA 3 cut(s) 420, 605, 1083
TspGWI ACGGA 1 cut(s) 1051
TspRI CASTG 1 cut(s) 469
VpaK11BI GGWCC 4 cut(s) 31, 610, 971, 1038
XapI RAATTY 1 cut(s) 988
XceI RCATGY 1 cut(s) 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.