MD12G1036600.v1.1

Heme-binding-like protein At3g10130

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008357
Physical Location & Seq
Forward (+)
3926376 .. 3928950
2575 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1036600.v1.1.491

Sequence Viewer

Length: 909 bp
ATGTCGTTCACTCACACGCACCCTTCCAAAACCCTAACCTGCACTTCCTTCTCCGGAAACCCTAACATCTTCAGACGACTCACCACCATCTCCATGGCGACGGACCGAGCCGCCGCCGTCCCCTCCCCGACGCAGCAGCGGAGGAGGACCATGTCCGCTCTAGAAGCCCGAGTCTCCCTCGTTGCCGCTCTCGCTTCTCAAGCGTCGTCTCTCTCCCAGCGACTTTTGCTCGAATTGGCCACGGAGACTGCAAAATACGTCTTCCCCAAACAGTTCGAGGCTCGTACTCTCGAAGAAGCCCTCATGGCAGTTCCGGACCTTGAAACGGTGAAGTTCAAAGTTCTGAGTAAGAGAGACCAATATGAGATCAGAGAAATTGAGCCTTACATTATAGCAGAGACAACAATGCCTGGAAAGACTGGTTTTGATTTCAATGGTGCATCTCAATCCTTCAATGTCTTAGCTGAATACCTGTTTGGTAAGAATACCACAAAGGAGAAAATGGAGATGACCACACCGGTTTATACACGTAAAGTTCAATCTGATGGGGAGAAAATGGAAATGACAACTCCTGTGATAACAAAGAGGCTGGGAGATCAAGATAAATGGCAGATGTCCTTTGTCATTCCCTCGAAATACGGTGCCAATGTGCCATTGCCTAAAGATCCATCTGTAAGGATCGAAGAGGTCCCTAGGAAAGTAGTTGCAGTTGTTGCCTTTTCAGGGTTTGTTACTAATGAGGAAGTTAAGAAACGAGAATCCAAGCTAAGGGAAGCTCTGAAAAATGATGGACAGTTCCAAGTGAAGGAGGGAACTTCAGTGGAAGTTGCACAGTATAATCCACCATTTACACTTCCATTTCAACGGCGTAATGAGATCAGTCTGGAAGTCGAAAGTAAAGAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

303

Amino Acids

34.05

Weight (kDa)

8.96

Isoelectric Point (pI)

53.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 107 - 295 2.7e-47 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 641
AccBSI CCGCTC 2 cut(s) 158, 188
AccIII TCCGGA 2 cut(s) 53, 313
AciI CCGC 5 cut(s) 111, 114, 139, 156, 186
AclWI GGATC 2 cut(s) 659, 686
AcoI YGGCCR 1 cut(s) 237
AcuI CTGAAG 2 cut(s) 55, 801
AfaI GTAC 1 cut(s) 286
AfiI CCNNNNNNNGG 4 cut(s) 325, 723, 768, 805
AflIII ACRYGT 1 cut(s) 527
AgeI ACCGGT 1 cut(s) 517
AgsI TTSAA 6 cut(s) 323, 337, 433, 454, 539, 863
AjnI CCWGG 1 cut(s) 409
AjuI GAANNNNNNNTTGG 2 cut(s) 459, 491
AluBI AGCT 3 cut(s) 464, 766, 776
AluI AGCT 3 cut(s) 464, 766, 776
Alw26I GTCTC 5 cut(s) 178, 213, 239, 348, 392
AlwI GGATC 2 cut(s) 659, 686
Ama87I CYCGRG 1 cut(s) 168
Aor13HI TCCGGA 2 cut(s) 53, 313
AoxI GGCC 1 cut(s) 237
ApeKI GCWGC 2 cut(s) 133, 136
AsiGI ACCGGT 1 cut(s) 517
AspA2I CCTAGG 1 cut(s) 692
AspS9I GGNCC 4 cut(s) 103, 147, 316, 688
AsuHPI GGTGA 2 cut(s) 73, 340
AvaI CYCGRG 1 cut(s) 168
AvaII GGWCC 4 cut(s) 103, 147, 316, 688
AvrII CCTAGG 1 cut(s) 692
BalI TGGCCA 1 cut(s) 239
BanI GGYRCC 1 cut(s) 641
BbsI GAAGAC 1 cut(s) 253
BbvI GCAGC 2 cut(s) 145, 148
BccI CCATC 4 cut(s) 95, 539, 676, 782
BceAI ACGGC 2 cut(s) 101, 881
BciT130I CCWGG 1 cut(s) 411
BcoDI GTCTC 5 cut(s) 178, 213, 239, 348, 392
BfaI CTAG 2 cut(s) 161, 693
BfuAI ACCTGC 1 cut(s) 47
BglI GCCNNNNNGGC 1 cut(s) 305
BisI GCNGC 5 cut(s) 111, 114, 134, 137, 186
BlnI CCTAGG 1 cut(s) 692
BlsI GCNGC 5 cut(s) 112, 115, 135, 138, 187
Bme1390I CCNGG 1 cut(s) 411
Bme18I GGWCC 4 cut(s) 103, 147, 316, 688
BmeT110I CYCGRG 1 cut(s) 168
BmgT120I GGNCC 4 cut(s) 103, 147, 316, 688
BmiI GGNNCC 2 cut(s) 643, 690
BmrFI CCNGG 1 cut(s) 411
BmsI GCATC 1 cut(s) 449
BpiI GAAGAC 1 cut(s) 253
BplI GAGNNNNNCTC 2 cut(s) 162, 194
Bpu10I CCTNAGC 1 cut(s) 767
BpuEI CTTGAG 1 cut(s) 183
BsaAI YACGTR 1 cut(s) 530
BsaI GGTCTC 1 cut(s) 348
BsaJI CCNNGG 3 cut(s) 93, 240, 692
BsaWI WCCGGW 3 cut(s) 53, 313, 517
Bsc4I CCNNNNNNNGG 4 cut(s) 325, 723, 768, 805
Bse118I RCCGGY 1 cut(s) 517
Bse1I ACTGG 1 cut(s) 424
Bse3DI GCAATG 1 cut(s) 653
BseAI TCCGGA 2 cut(s) 53, 313
BseBI CCWGG 1 cut(s) 411
BseDI CCNNGG 3 cut(s) 93, 240, 692
BseLI CCNNNNNNNGG 4 cut(s) 325, 723, 768, 805
BseMI GCAATG 1 cut(s) 653
BseMII CTCAG 1 cut(s) 335
BseNI ACTGG 1 cut(s) 424
BseRI GAGGAG 1 cut(s) 157
BseXI GCAGC 2 cut(s) 145, 148
BseYI CCCAGC 2 cut(s) 216, 589
BsgI GTGCAG 1 cut(s) 25
BshFI GGCC 1 cut(s) 239
BshNI GGYRCC 1 cut(s) 641
BshTI ACCGGT 1 cut(s) 517
BsiHKCI CYCGRG 1 cut(s) 168
BsiSI CCGG 3 cut(s) 54, 314, 518
BslFI GGGAC 2 cut(s) 104, 674
BslI CCNNNNNNNGG 4 cut(s) 325, 723, 768, 805
BsmAI GTCTC 5 cut(s) 178, 213, 239, 348, 392
BsmBI CGTCTC 1 cut(s) 213
BsmFI GGGAC 2 cut(s) 104, 674
BsnI GGCC 1 cut(s) 239
Bso31I GGTCTC 1 cut(s) 348
BsoBI CYCGRG 1 cut(s) 168
Bsp13I TCCGGA 2 cut(s) 53, 313
Bsp143I GATC 5 cut(s) 366, 595, 664, 678, 876
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 5 cut(s) 111, 114, 139, 156, 186
BspANI GGCC 1 cut(s) 239
BspCNI CTCAG 1 cut(s) 336
BspEI TCCGGA 2 cut(s) 53, 313
BspLI GGNNCC 2 cut(s) 643, 690
BspMI ACCTGC 1 cut(s) 47
BspPI GGATC 2 cut(s) 659, 686
BspT107I GGYRCC 1 cut(s) 641
BspTNI GGTCTC 1 cut(s) 348
BsrBI CCGCTC 2 cut(s) 158, 188
BsrDI GCAATG 1 cut(s) 653
BsrFI RCCGGY 1 cut(s) 517
BsrI ACTGG 1 cut(s) 424
BssAI RCCGGY 1 cut(s) 517
BssECI CCNNGG 3 cut(s) 93, 240, 692
BssMI GATC 5 cut(s) 366, 595, 664, 678, 876
BssT1I CCWWGG 2 cut(s) 93, 692
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 5 cut(s) 273, 328, 641, 795, 834
Bst6I CTCTTC 1 cut(s) 678
BstAPI GCANNNNNTGC 1 cut(s) 713
BstBAI YACGTR 1 cut(s) 530
BstDEI CTNAG 3 cut(s) 344, 460, 767
BstDSI CCRYGG 2 cut(s) 93, 240
BstKTI GATC 5 cut(s) 369, 598, 667, 681, 879
BstMAI GTCTC 5 cut(s) 178, 213, 239, 348, 392
BstMBI GATC 5 cut(s) 366, 595, 664, 678, 876
BstMWI GCNNNNNNNGC 6 cut(s) 164, 191, 200, 226, 305, 713
BstNI CCWGG 1 cut(s) 411
BstSCI CCNGG 1 cut(s) 409
BstV1I GCAGC 2 cut(s) 145, 148
BstV2I GAAGAC 1 cut(s) 253
BstX2I RGATCY 1 cut(s) 664
BstXI CCANNNNNNTGG 1 cut(s) 94
BstYI RGATCY 1 cut(s) 664
BsuRI GGCC 1 cut(s) 239
BtgI CCRYGG 2 cut(s) 93, 240
BtsIMutI CAGTG 1 cut(s) 825
BveI ACCTGC 1 cut(s) 47
Cfr10I RCCGGY 1 cut(s) 517
Cfr13I GGNCC 4 cut(s) 103, 147, 316, 688
CpoI CGGWCCG 1 cut(s) 103
CseI GACGC 2 cut(s) 139, 192
Csp6I GTAC 1 cut(s) 285
CspAI ACCGGT 1 cut(s) 517
CspI CGGWCCG 1 cut(s) 103
CviAII CATG 3 cut(s) 94, 151, 304
CviQI GTAC 1 cut(s) 285
DdeI CTNAG 3 cut(s) 344, 460, 767
DpnI GATC 5 cut(s) 368, 597, 666, 680, 878
DpnII GATC 5 cut(s) 366, 595, 664, 678, 876
EaeI YGGCCR 1 cut(s) 237
Eam1104I CTCTTC 1 cut(s) 678
EarI CTCTTC 1 cut(s) 678
Eco130I CCWWGG 2 cut(s) 93, 692
Eco31I GGTCTC 1 cut(s) 348
Eco47I GGWCC 4 cut(s) 103, 147, 316, 688
Eco57I CTGAAG 2 cut(s) 55, 801
Eco88I CYCGRG 1 cut(s) 168
EcoO109I RGGNCCY 1 cut(s) 688
EcoRII CCWGG 1 cut(s) 409
EcoT14I CCWWGG 2 cut(s) 93, 692
ErhI CCWWGG 2 cut(s) 93, 692
Esp3I CGTCTC 1 cut(s) 213
FaeI CATG 3 cut(s) 97, 154, 307
FaiI YATR 7 cut(s) 95, 152, 305, 363, 392, 525, 837
FaqI GGGAC 2 cut(s) 104, 674
FatI CATG 3 cut(s) 93, 150, 303
Fnu4HI GCNGC 5 cut(s) 111, 114, 134, 137, 186
Fsp4HI GCNGC 5 cut(s) 111, 114, 134, 137, 186
FspBI CTAG 2 cut(s) 161, 693
GluI GCNGC 5 cut(s) 111, 114, 134, 137, 186
GsaI CCCAGC 2 cut(s) 220, 593
HaeIII GGCC 1 cut(s) 239
HapII CCGG 3 cut(s) 54, 314, 518
HgaI GACGC 2 cut(s) 139, 192
Hin1II CATG 3 cut(s) 97, 154, 307
HinfI GANTC 3 cut(s) 78, 171, 758
HpaII CCGG 3 cut(s) 54, 314, 518
HphI GGTGA 2 cut(s) 73, 340
Hpy166II GTNNAC 1 cut(s) 9
Hpy188I TCNGA 5 cut(s) 74, 345, 371, 544, 780
Hpy188III TCNNGA 6 cut(s) 54, 161, 290, 314, 599, 884
Hpy8I GTNNAC 1 cut(s) 9
Hpy99I CGWCG 3 cut(s) 103, 133, 208
HpyAV CCTTC 4 cut(s) 33, 58, 460, 799
HpyCH4III ACNGT 5 cut(s) 273, 328, 641, 795, 834
HpyCH4IV ACGT 2 cut(s) 258, 529
HpyCH4V TGCA 5 cut(s) 42, 251, 440, 707, 830
HpyF10VI GCNNNNNNNGC 6 cut(s) 164, 191, 200, 226, 305, 713
HpyF3I CTNAG 3 cut(s) 344, 460, 767
HpySE526I ACGT 2 cut(s) 258, 529
Hsp92II CATG 3 cut(s) 97, 154, 307
Kpn2I TCCGGA 2 cut(s) 53, 313
Kzo9I GATC 5 cut(s) 366, 595, 664, 678, 876
Lsp1109I GCAGC 2 cut(s) 145, 148
LweI GCATC 1 cut(s) 449
MaeI CTAG 2 cut(s) 161, 693
MaeII ACGT 2 cut(s) 258, 529
MaeIII GTNAC 1 cut(s) 730
MalI GATC 5 cut(s) 368, 597, 666, 680, 878
MbiI CCGCTC 2 cut(s) 158, 188
MboI GATC 5 cut(s) 366, 595, 664, 678, 876
MboII GAAGA 4 cut(s) 61, 253, 305, 695
MflI RGATCY 1 cut(s) 664
MlsI TGGCCA 1 cut(s) 239
MluCI AATT 2 cut(s) 233, 375
MluNI TGGCCA 1 cut(s) 239
MlyI GAGTC 2 cut(s) 72, 180
Mox20I TGGCCA 1 cut(s) 239
MroI TCCGGA 2 cut(s) 53, 313
MscI TGGCCA 1 cut(s) 239
MseI TTAA 1 cut(s) 747
MslI CAYNNNNRTG 1 cut(s) 92
Msp20I TGGCCA 1 cut(s) 239
MspA1I CMGCKG 1 cut(s) 139
MspI CCGG 3 cut(s) 54, 314, 518
MspR9I CCNGG 1 cut(s) 411
MvaI CCWGG 1 cut(s) 411
MwoI GCNNNNNNNGC 6 cut(s) 164, 191, 200, 226, 305, 713
NcoI CCATGG 1 cut(s) 93
NdeII GATC 5 cut(s) 366, 595, 664, 678, 876
NlaIII CATG 3 cut(s) 97, 154, 307
NlaIV GGNNCC 2 cut(s) 643, 690
PfeI GAWTC 1 cut(s) 758
PflFI GACNNNGTC 1 cut(s) 151
PinAI ACCGGT 1 cut(s) 517
PkrI GCNGC 5 cut(s) 112, 115, 135, 138, 187
PleI GAGTC 2 cut(s) 72, 179
PpsI GAGTC 2 cut(s) 72, 179
Ppu21I YACGTR 1 cut(s) 530
PpuMI RGGWCCY 1 cut(s) 688
Psp5II RGGWCCY 1 cut(s) 688
Psp6I CCWGG 1 cut(s) 409
PspFI CCCAGC 2 cut(s) 216, 589
PspGI CCWGG 1 cut(s) 409
PspN4I GGNNCC 2 cut(s) 643, 690
PspPI GGNCC 4 cut(s) 103, 147, 316, 688
PspPPI RGGWCCY 1 cut(s) 688
PsuI RGATCY 1 cut(s) 664
PsyI GACNNNGTC 1 cut(s) 151
RsaI GTAC 1 cut(s) 286
RsaNI GTAC 1 cut(s) 285
RseI CAYNNNNRTG 1 cut(s) 92
Rsr2I CGGWCCG 1 cut(s) 103
RsrII CGGWCCG 1 cut(s) 103
SaqAI TTAA 1 cut(s) 747
SatI GCNGC 5 cut(s) 111, 114, 134, 137, 186
Sau3AI GATC 5 cut(s) 366, 595, 664, 678, 876
Sau96I GGNCC 4 cut(s) 103, 147, 316, 688
SchI GAGTC 2 cut(s) 72, 180
ScrFI CCNGG 1 cut(s) 411
SetI ASST 9 cut(s) 41, 261, 321, 466, 474, 532, 690, 768, 778
SfaNI GCATC 1 cut(s) 449
SinI GGWCC 4 cut(s) 103, 147, 316, 688
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
Sse9I AATT 2 cut(s) 233, 375
SsiI CCGC 5 cut(s) 111, 114, 139, 156, 186
SspMI CTAG 2 cut(s) 161, 693
StyD4I CCNGG 1 cut(s) 409
StyI CCWWGG 2 cut(s) 93, 692
TaaI ACNGT 5 cut(s) 273, 328, 641, 795, 834
TaiI ACGT 2 cut(s) 261, 532
TaqI TCGA 6 cut(s) 231, 276, 291, 632, 681, 891
TaqII GACCGA 1 cut(s) 120
TasI AATT 2 cut(s) 233, 375
TauI GCSGC 3 cut(s) 113, 116, 188
TfiI GAWTC 1 cut(s) 758
Tru1I TTAA 1 cut(s) 747
Tru9I TTAA 1 cut(s) 747
TscAI CASTG 1 cut(s) 825
TseI GCWGC 2 cut(s) 133, 136
TspGWI ACGGA 2 cut(s) 116, 257
TspRI CASTG 1 cut(s) 825
Tth111I GACNNNGTC 1 cut(s) 151
VpaK11BI GGWCC 4 cut(s) 103, 147, 316, 688
XbaI TCTAGA 1 cut(s) 160
XcmI CCANNNNNNNNNTGG 1 cut(s) 91
XmaJI CCTAGG 1 cut(s) 692
XspI CTAG 2 cut(s) 161, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.