Rroxscaffold_3G00219750

Heme-binding-like protein At3g10130

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008357
Physical Location & Seq
Reverse (-)
1872386 .. 1873618
1233 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_3G00219750.1

Sequence Viewer

Length: 399 bp
ATGTCGGGAAAGACTGGCTTTGATTTCAATGGTGCTTCACAATCATTCAATGTCTTAGCTGAATACCTGTTTGGTAAGAATACCACAAAGGAGAAAATGGAGATGACCACACCGGTTTTTATGCATAAAACTCAATCTGATGAAGAGAAAATGGAAATGACAACTCCAACGATAACAAACACTTGTAAAGGCACAGACTATATTGCCAAGCTTTGCCCTAAGAAAGCTGAGTGGGATAAGCACAAGAATTGCAGAAAGAAGAATGAGAATGATATGGAGGGAAGTTATGTTATAATTGTGGGGAAACTGGGCATTCGTTTTCTAGATACCCCTTACCTCTTCAAGATGGAGGAACTAAATTTGCCAAGTGATTCATCTATAACGAGACTAGTCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

132

Amino Acids

15.07

Weight (kDa)

7.67

Isoelectric Point (pI)

33.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 10 - 53 3.2e-08 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 293
AcsI RAATTY 1 cut(s) 358
AgeI ACCGGT 1 cut(s) 112
AgsI TTSAA 3 cut(s) 28, 49, 343
AhlI ACTAGT 1 cut(s) 388
AjuI GAANNNNNNNTTGG 2 cut(s) 54, 86
AluBI AGCT 3 cut(s) 59, 211, 227
AluI AGCT 3 cut(s) 59, 211, 227
Alw26I GTCTC 1 cut(s) 379
ApoI RAATTY 1 cut(s) 358
AsiGI ACCGGT 1 cut(s) 112
AsuHPI GGTGA 1 cut(s) 385
BccI CCATC 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 379
BcuI ACTAGT 1 cut(s) 388
BfaI CTAG 2 cut(s) 323, 389
BmrI ACTGGG 1 cut(s) 317
BmuI ACTGGG 1 cut(s) 317
BsaWI WCCGGW 1 cut(s) 112
Bse118I RCCGGY 1 cut(s) 112
Bse1I ACTGG 2 cut(s) 19, 312
BseMII CTCAG 1 cut(s) 219
BseNI ACTGG 2 cut(s) 19, 312
BshTI ACCGGT 1 cut(s) 112
BsiSI CCGG 1 cut(s) 113
BsmAI GTCTC 1 cut(s) 379
BsmI GAATGC 1 cut(s) 312
BspCNI CTCAG 1 cut(s) 220
BsrFI RCCGGY 1 cut(s) 112
BsrI ACTGG 2 cut(s) 19, 312
BssAI RCCGGY 1 cut(s) 112
Bst6I CTCTTC 2 cut(s) 138, 344
BstDEI CTNAG 3 cut(s) 55, 219, 228
BstMAI GTCTC 1 cut(s) 379
Cfr10I RCCGGY 1 cut(s) 112
CspAI ACCGGT 1 cut(s) 112
CviJI RGCY 4 cut(s) 18, 59, 211, 227
CviKI_1 RGCY 4 cut(s) 18, 59, 211, 227
DdeI CTNAG 3 cut(s) 55, 219, 228
Eam1104I CTCTTC 2 cut(s) 138, 344
EarI CTCTTC 2 cut(s) 138, 344
EcoT22I ATGCAT 1 cut(s) 126
FaiI YATR 7 cut(s) 122, 126, 201, 275, 288, 293, 380
FalI AAGNNNNNCTT 1 cut(s) 34
FspBI CTAG 2 cut(s) 323, 389
HapII CCGG 1 cut(s) 113
HindIII AAGCTT 1 cut(s) 209
HinfI GANTC 1 cut(s) 371
HpaII CCGG 1 cut(s) 113
HphI GGTGA 1 cut(s) 385
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 3 cut(s) 6, 323, 343
HpyCH4V TGCA 2 cut(s) 124, 252
HpyF3I CTNAG 3 cut(s) 55, 219, 228
LpnPI CCDG 3 cut(s) 80, 126, 293
MaeI CTAG 2 cut(s) 323, 389
MaeIII GTNAC 1 cut(s) 391
MboII GAAGA 3 cut(s) 155, 271, 331
MluCI AATT 3 cut(s) 247, 294, 358
MmeI TCCRAC 1 cut(s) 191
MnlI CCTC 3 cut(s) 271, 343, 347
Mph1103I ATGCAT 1 cut(s) 126
MspI CCGG 1 cut(s) 113
Mva1269I GAATGC 1 cut(s) 312
NmuCI GTSAC 1 cut(s) 391
NsiI ATGCAT 1 cut(s) 126
PctI GAATGC 1 cut(s) 312
PfeI GAWTC 1 cut(s) 371
PinAI ACCGGT 1 cut(s) 112
PsiI TTATAA 1 cut(s) 293
SetI ASST 6 cut(s) 61, 69, 213, 229, 339, 398
SpeI ACTAGT 1 cut(s) 388
Sse9I AATT 3 cut(s) 247, 294, 358
SspMI CTAG 2 cut(s) 323, 389
TasI AATT 3 cut(s) 247, 294, 358
TfiI GAWTC 1 cut(s) 371
TseFI GTSAC 1 cut(s) 391
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 2 cut(s) 156, 363
XapI RAATTY 1 cut(s) 358
XbaI TCTAGA 1 cut(s) 322
XspI CTAG 2 cut(s) 323, 389
Zsp2I ATGCAT 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.