Rh3BG214600

Heme-binding-like protein At3g10130

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0008357
Physical Location & Seq
Forward (+)
19352751 .. 19355613
2863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG214600.1

Sequence Viewer

Length: 684 bp
ATGCTTCTTCACCAAGCTCCAATGTTCACCTGTACTACTCCCCTCACACGAAACCCTAACCGACTCACCGCCACAACCTCCATGGCCGCCGACCGCGCCGCAGCACCTTCTCCGCCGCAGCGAAGAAGGACCATGTCCGCTCTCGAAGCCCGAATCTCCCTCGTCGCCGCTCTCGCCGCCCAAGCCTCCACCGTCTCGCAGCGACTTTTGCTCGATTTGGCCGCCGAGACGGCCAAGTACGTCTTCCCCAAGCAGTTCGAGGCTCGGACTCTCGAGGAAGCTCTCATGTCAGTTCCGGACCTGGAAACGGTGAAGTTCAAAGTGTTGAGTAGGAGAGACCAGTATGAGATTAGAGAAGTTGAGGTGGAAAATGAAGATAAGTGGCAGATGTCCTTTGTCATGCCCTCAAAATACGGTGCCAATTTGCCATTGCCTAAAGATCCATCTGTAAAGGTCAAAGAGGTTCCAAAGAAATTAGTTGCAGTTGTTGCCTTTTCTGGTTTTGTCACTGATGAGGAAATTAAGCAACGGGAATCCAAGTTAAGGGAAGCTTTGAAAGATGATAGACAGTTCCAAGTGAAGCAGGGCACTTCAGTGGAAGTTGCACAGTATAACCCACCATTTACGCTTCCATTTCAACGGCGTAATGAGATCAGTCTGGAAGTTGAAAGTAAAGAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

227

Amino Acids

25.69

Weight (kDa)

8.59

Isoelectric Point (pI)

53.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 124 - 220 3.4e-27 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 416
AccBSI CCGCTC 2 cut(s) 140, 170
AccII CGCG 1 cut(s) 96
AccIII TCCGGA 1 cut(s) 295
AclWI GGATC 1 cut(s) 434
AcoI YGGCCR 3 cut(s) 84, 219, 231
AcuI CTGAAG 1 cut(s) 576
AfaI GTAC 2 cut(s) 34, 239
AfiI CCNNNNNNNGG 2 cut(s) 307, 543
AgsI TTSAA 4 cut(s) 319, 556, 638, 668
AjnI CCWGG 1 cut(s) 300
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AleI CACNNNNGTG 1 cut(s) 593
AluBI AGCT 3 cut(s) 17, 281, 551
AluI AGCT 3 cut(s) 17, 281, 551
Alw26I GTCTC 3 cut(s) 199, 221, 330
AlwI GGATC 1 cut(s) 434
Ama87I CYCGRG 1 cut(s) 272
Aor13HI TCCGGA 1 cut(s) 295
AoxI GGCC 3 cut(s) 84, 219, 231
ApeKI GCWGC 3 cut(s) 101, 118, 199
AspLEI GCGC 1 cut(s) 98
AspS9I GGNCC 2 cut(s) 129, 298
AsuHPI GGTGA 3 cut(s) 19, 58, 322
AvaI CYCGRG 1 cut(s) 272
AvaII GGWCC 2 cut(s) 129, 298
BaeGI GKGCMC 1 cut(s) 590
BanI GGYRCC 1 cut(s) 416
BbsI GAAGAC 1 cut(s) 235
BbvI GCAGC 3 cut(s) 113, 130, 211
BccI CCATC 1 cut(s) 451
BceAI ACGGC 2 cut(s) 246, 656
BciT130I CCWGG 1 cut(s) 302
BcoDI GTCTC 3 cut(s) 199, 221, 330
BglI GCCNNNNNGGC 1 cut(s) 230
BisI GCNGC 9 cut(s) 87, 99, 102, 116, 119, 168, 177, 200, 222
BlsI GCNGC 9 cut(s) 88, 100, 103, 117, 120, 169, 178, 201, 223
Bme1390I CCNGG 1 cut(s) 302
Bme18I GGWCC 2 cut(s) 129, 298
BmeT110I CYCGRG 1 cut(s) 272
BmgT120I GGNCC 2 cut(s) 129, 298
BmiI GGNNCC 2 cut(s) 418, 465
BmrFI CCNGG 1 cut(s) 302
BpiI GAAGAC 1 cut(s) 235
BsaI GGTCTC 1 cut(s) 330
BsaJI CCNNGG 1 cut(s) 81
BsaWI WCCGGW 1 cut(s) 295
Bsc4I CCNNNNNNNGG 2 cut(s) 307, 543
Bse1I ACTGG 1 cut(s) 340
Bse3DI GCAATG 1 cut(s) 428
BseAI TCCGGA 1 cut(s) 295
BseBI CCWGG 1 cut(s) 302
BseDI CCNNGG 1 cut(s) 81
BseLI CCNNNNNNNGG 2 cut(s) 307, 543
BseMI GCAATG 1 cut(s) 428
BseNI ACTGG 1 cut(s) 340
BseSI GKGCMC 1 cut(s) 590
BseXI GCAGC 3 cut(s) 113, 130, 211
Bsh1236I CGCG 1 cut(s) 96
Bsh1285I CGRYCG 1 cut(s) 94
BshFI GGCC 3 cut(s) 86, 221, 233
BshNI GGYRCC 1 cut(s) 416
BsiEI CGRYCG 1 cut(s) 94
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 1 cut(s) 296
BslI CCNNNNNNNGG 2 cut(s) 307, 543
BsmAI GTCTC 3 cut(s) 199, 221, 330
BsmBI CGTCTC 2 cut(s) 199, 221
BsnI GGCC 3 cut(s) 86, 221, 233
Bso31I GGTCTC 1 cut(s) 330
BsoBI CYCGRG 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 590
Bsp13I TCCGGA 1 cut(s) 295
Bsp143I GATC 2 cut(s) 439, 651
Bsp19I CCATGG 1 cut(s) 81
BspANI GGCC 3 cut(s) 86, 221, 233
BspEI TCCGGA 1 cut(s) 295
BspFNI CGCG 1 cut(s) 96
BspLI GGNNCC 2 cut(s) 418, 465
BspPI GGATC 1 cut(s) 434
BspT107I GGYRCC 1 cut(s) 416
BspTNI GGTCTC 1 cut(s) 330
BsrBI CCGCTC 2 cut(s) 140, 170
BsrDI GCAATG 1 cut(s) 428
BsrI ACTGG 1 cut(s) 340
BssECI CCNNGG 1 cut(s) 81
BssMI GATC 2 cut(s) 439, 651
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 5 cut(s) 193, 310, 416, 570, 609
BstAPI GCANNNNNTGC 1 cut(s) 488
BstDSI CCRYGG 1 cut(s) 81
BstFNI CGCG 1 cut(s) 96
BstHHI GCGC 1 cut(s) 98
BstKTI GATC 2 cut(s) 442, 654
BstMAI GTCTC 3 cut(s) 199, 221, 330
BstMBI GATC 2 cut(s) 439, 651
BstMCI CGRYCG 1 cut(s) 94
BstMWI GCNNNNNNNGC 8 cut(s) 95, 146, 173, 176, 182, 208, 230, 488
BstNI CCWGG 1 cut(s) 302
BstSCI CCNGG 1 cut(s) 300
BstSLI GKGCMC 1 cut(s) 590
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 3 cut(s) 113, 130, 211
BstV2I GAAGAC 1 cut(s) 235
BstX2I RGATCY 1 cut(s) 439
BstYI RGATCY 1 cut(s) 439
BsuRI GGCC 3 cut(s) 86, 221, 233
BtgI CCRYGG 1 cut(s) 81
BtsIMutI CAGTG 2 cut(s) 507, 600
CfoI GCGC 1 cut(s) 98
Cfr13I GGNCC 2 cut(s) 129, 298
Csp6I GTAC 2 cut(s) 33, 238
CviAII CATG 4 cut(s) 82, 133, 286, 400
CviJI RGCY 9 cut(s) 17, 86, 149, 185, 221, 233, 263, 281, 551
CviKI_1 RGCY 9 cut(s) 17, 86, 149, 185, 221, 233, 263, 281, 551
CviQI GTAC 2 cut(s) 33, 238
DpnI GATC 2 cut(s) 441, 653
DpnII GATC 2 cut(s) 439, 651
EaeI YGGCCR 3 cut(s) 84, 219, 231
EciI GGCGGA 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 81
Eco31I GGTCTC 1 cut(s) 330
Eco47I GGWCC 2 cut(s) 129, 298
Eco57I CTGAAG 1 cut(s) 576
Eco88I CYCGRG 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 300
EcoT14I CCWWGG 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 2 cut(s) 199, 221
FaeI CATG 4 cut(s) 85, 136, 289, 403
FaiI YATR 6 cut(s) 83, 134, 287, 345, 401, 612
FalI AAGNNNNNCTT 4 cut(s) 227, 259, 535, 567
FatI CATG 4 cut(s) 81, 132, 285, 399
Fnu4HI GCNGC 9 cut(s) 87, 99, 102, 116, 119, 168, 177, 200, 222
Fsp4HI GCNGC 9 cut(s) 87, 99, 102, 116, 119, 168, 177, 200, 222
GlaI GCGC 1 cut(s) 97
GluI GCNGC 9 cut(s) 87, 99, 102, 116, 119, 168, 177, 200, 222
HaeIII GGCC 3 cut(s) 86, 221, 233
HapII CCGG 1 cut(s) 296
HhaI GCGC 1 cut(s) 98
Hin1II CATG 4 cut(s) 85, 136, 289, 403
Hin6I GCGC 1 cut(s) 96
HinP1I GCGC 1 cut(s) 96
HindIII AAGCTT 1 cut(s) 549
HinfI GANTC 4 cut(s) 63, 153, 268, 533
HpaII CCGG 1 cut(s) 296
HphI GGTGA 3 cut(s) 19, 58, 322
Hpy166II GTNNAC 1 cut(s) 27
Hpy188I TCNGA 1 cut(s) 267
Hpy188III TCNNGA 4 cut(s) 143, 272, 296, 659
Hpy8I GTNNAC 1 cut(s) 27
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 2 cut(s) 117, 120
HpyCH4III ACNGT 5 cut(s) 193, 310, 416, 570, 609
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 2 cut(s) 482, 605
HpyF10VI GCNNNNNNNGC 8 cut(s) 95, 146, 173, 176, 182, 208, 230, 488
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 4 cut(s) 85, 136, 289, 403
HspAI GCGC 1 cut(s) 96
Kpn2I TCCGGA 1 cut(s) 295
Kzo9I GATC 2 cut(s) 439, 651
LmnI GCTCC 1 cut(s) 22
LpnPI CCDG 8 cut(s) 43, 287, 309, 314, 353, 483, 569, 644
Lsp1109I GCAGC 3 cut(s) 113, 130, 211
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 1 cut(s) 505
MalI GATC 2 cut(s) 441, 653
MbiI CCGCTC 2 cut(s) 140, 170
MboI GATC 2 cut(s) 439, 651
MboII GAAGA 3 cut(s) 135, 235, 386
MflI RGATCY 1 cut(s) 439
MhlI GDGCHC 1 cut(s) 590
MluCI AATT 3 cut(s) 421, 473, 519
MlyI GAGTC 2 cut(s) 57, 262
MroI TCCGGA 1 cut(s) 295
MseI TTAA 2 cut(s) 522, 542
MslI CAYNNNNRTG 1 cut(s) 593
MspI CCGG 1 cut(s) 296
MspR9I CCNGG 1 cut(s) 302
MvaI CCWGG 1 cut(s) 302
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 8 cut(s) 95, 146, 173, 176, 182, 208, 230, 488
NcoI CCATGG 1 cut(s) 81
NdeII GATC 2 cut(s) 439, 651
NlaIII CATG 4 cut(s) 85, 136, 289, 403
NlaIV GGNNCC 2 cut(s) 418, 465
NmeAIII GCCGAG 1 cut(s) 250
NmuCI GTSAC 1 cut(s) 505
OliI CACNNNNGTG 1 cut(s) 593
PaeR7I CTCGAG 1 cut(s) 272
PfeI GAWTC 2 cut(s) 153, 533
PflFI GACNNNGTC 1 cut(s) 133
PkrI GCNGC 9 cut(s) 88, 100, 103, 117, 120, 169, 178, 201, 223
PleI GAGTC 2 cut(s) 57, 262
PpsI GAGTC 2 cut(s) 57, 262
Psp6I CCWGG 1 cut(s) 300
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 2 cut(s) 418, 465
PspPI GGNCC 2 cut(s) 129, 298
PsuI RGATCY 1 cut(s) 439
PsyI GACNNNGTC 1 cut(s) 133
RsaI GTAC 2 cut(s) 34, 239
RsaNI GTAC 2 cut(s) 33, 238
RseI CAYNNNNRTG 1 cut(s) 593
SaqAI TTAA 2 cut(s) 522, 542
SatI GCNGC 9 cut(s) 87, 99, 102, 116, 119, 168, 177, 200, 222
Sau3AI GATC 2 cut(s) 439, 651
Sau96I GGNCC 2 cut(s) 129, 298
SchI GAGTC 2 cut(s) 57, 262
ScrFI CCNGG 1 cut(s) 302
SduI GDGCHC 1 cut(s) 590
Sfr274I CTCGAG 1 cut(s) 272
SinI GGWCC 2 cut(s) 129, 298
SlaI CTCGAG 1 cut(s) 272
SmiMI CAYNNNNRTG 1 cut(s) 593
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 3 cut(s) 421, 473, 519
StyD4I CCNGG 1 cut(s) 300
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 5 cut(s) 193, 310, 416, 570, 609
TaiI ACGT 1 cut(s) 243
TaqI TCGA 4 cut(s) 144, 213, 258, 273
TasI AATT 3 cut(s) 421, 473, 519
TatI WGTACW 1 cut(s) 32
TauI GCSGC 6 cut(s) 89, 101, 118, 170, 179, 224
TfiI GAWTC 2 cut(s) 153, 533
Tru1I TTAA 2 cut(s) 522, 542
Tru9I TTAA 2 cut(s) 522, 542
TscAI CASTG 2 cut(s) 514, 600
TseFI GTSAC 1 cut(s) 505
TseI GCWGC 3 cut(s) 101, 118, 199
Tsp45I GTSAC 1 cut(s) 505
TspDTI ATGAA 1 cut(s) 387
TspRI CASTG 2 cut(s) 514, 600
Tth111I GACNNNGTC 1 cut(s) 133
VpaK11BI GGWCC 2 cut(s) 129, 298
XcmI CCANNNNNNNNNTGG 1 cut(s) 79
XhoI CTCGAG 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.