Rorug03G0135700

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004049
Physical Location & Seq
Forward (+)
11160682 .. 11163617
2936 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0135700.1

Sequence Viewer

Length: 2025 bp
ATGTTTGTCAAGCTTTCTGTTCTCCTAGTACTACTAGTATTTCTGGCACCCACAGAAGCCCAAGACCTCAATTTCATCTACAATGATGGTTTCACTGGCCGTTCTGGTCTTAATCTCAGTCTAGACGGCATAGCAGAGATCACACCAAAAGGTCTCTTGAAGCTTACAAACCACACCAAACTGAGAAGTGGTCATGCCTTCTACCCTAACCCAGTAACCTTCAAGAACTCAGAGAACAACACCGCTTTCTCCTTCTCCACCAAATTTATCTTTGCCATCCAATCAGAGTACACTACTTTCAGCGGCCATGGAATCGCCTTTGTCATCGCTCCGACGAGAGGCCTTCCCGGAGCTCTGTCGAACCAGTACCTGAGCCTGTTCAACGTCTCCAACAATGGGAAATTCAGCAATCATGTTTTTGCTGTGGAGCTTGACACGCAACAGAACACGGAATTCAATGACATCAATGAAAACCATGTTGGGATAGACATCAATGGCTTGCGCTCTGTCAAAGCTGCCCCAGCTGGTTATTTTGATGGTCAGTACTTCAAGAACCTAACTCTTAACAGTGGTAAAGAAATGAGAGTCTGGGTTGAATATGATGGTACCAAGAAGCAAATTGAAGTTACTATGGCTCCAATTGCTGTTGCAACTAAACCCCCAACTCCACTTTTGTCTTTGAAATATGACCTTTCCCCAATTCTCAACAAAACCATGTATGTTGGCTTTTCCTCTTCAACTGGTTCGTTCCTGACATCCCATTATGTAGTGGGTTGGAGCTTTAGGATGAATGGCCAAGCTCAAGACCTTATAGCTTCCAAACTTCCCAAGTTGCCTAGCATTGCAGGTAAAAAGAGGTCCATGCTTTTCACTTTTGGTGTGCCTCTGATTTCAGTGAATTTGGTTTTGCTAGTGGTTTCTGGGGTGCTTTATGTCATAAGAAGGAAGAGGAAGTTTGCAGAAGTGCTTGAAGATTGGGAGCTAGAGTATGGTCCTCAGAGGTTTAAGTACAAGGAACTGTATATAGCCACCAAAGGGTTTAGGGAAAAGGAGCTTTTGGGAAATGGGGGATTTGGTAAAGTTTATAGAGGTTTATTACCCTCCTCTGAAGTTGAGATAGCAGTGAAGAGGGTATCACATGAATCAAGACAGGGGATGAAGGAATTTGTAGCAGAAATTGTTAGTATTGGCCGGCTTCGTCACCGGAATTTAGTACAACTGTTGGGATATTGCAGGCGAAAAGGGGAGCTGCTTTTGGTCTATGACTACATGCCTAATGGAAGCTTGGACAAATACCTCTTTGATCAACCTGAGGTGACCCTTAATTGGAGCCAGAGGTTTAAAGTCATCAAAGGTGTGGCTTCAGGGCTGTTCTATCTTCATGAAGAATGGGAACAGGTTGTGATTCATAGAGATGTGAAGGCCAGTAATGTATTGCTAGATGGGGAATTCAATGGAAGGCTAGGAGATTTCGGGCTTGCAAGATTATACGACCATGGAACAGACCCTCAAACTACTCATATAGTTGGAACACTCGGGTACCTAGCCCCAGAGCACACAAGAACAGGTCGGGCCACCACGAGCACCGACGTGTTTGCTTTTGGGGCATTTTTGCTCGAAGTTGCTTGTGGAAAAAGGCCAATCAAGACACAGGGTCCTGAAGATGTGATTTTGGTTGTTTGGGTGTTTTCTTGTTGGAATAGAAGTAACATCCTTGAGGCAAGAGATCAGAGCTTTGGTACGGATTTTGTCGCCGAGGAAGTGGAGTTGGTGTTGAAGCTTGGGCTTTTATGCTCTCATTCGGAGCCAGCGGCAAGGCCAAGCATGCGACAAGTCGTTCAGTACTTGGCTGGTGATGTTGCTTTGCCGGAAGTGTCACTTCTCGGGCTTTCTTCTAGCGGCTTAACTGTTGGACACCGTGAATGTTTTGATGACTATGCTATGTCGTATCAGTCTTCCTTAGGTAAGGGGTCCTTGCATTCATCATATATTGCAGAGTCGGCACTACTTTCAGGTGGTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

674

Amino Acids

74.87

Weight (kDa)

8.63

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 23 - 276 3.7e-82 Legume lectin domain
Lectin_L-type_dom PF18483 42 - 254 2.4e-09 Legume lectin beta-barrel domain
Pkinase PF00069 347 - 614 6.6e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 350 - 616 2.2e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 836
Acc65I GGTACC 2 cut(s) 605, 1539
AccB1I GGYRCC 3 cut(s) 46, 605, 1539
AciI CCGC 4 cut(s) 243, 303, 1811, 1899
AcoI YGGCCR 4 cut(s) 97, 304, 793, 1189
AcsI RAATTY 7 cut(s) 263, 401, 452, 898, 1163, 1207, 1448
AcuI CTGAAG 3 cut(s) 1128, 1347, 1680
AfiI CCNNNNNNNGG 4 cut(s) 338, 396, 1035, 1326
AflIII ACRYGT 1 cut(s) 1590
AhdI GACNNNNNGTC 1 cut(s) 1653
AhlI ACTAGT 1 cut(s) 34
AjiI CACGTC 1 cut(s) 1591
AjuI GAANNNNNNNTTGG 2 cut(s) 462, 494
AleI CACNNNNGTG 1 cut(s) 1589
Alw21I GWGCWC 3 cut(s) 355, 1557, 1586
Alw26I GTCTC 2 cut(s) 158, 391
AlwNI CAGNNNCTG 1 cut(s) 370
Ama87I CYCGRG 2 cut(s) 1535, 1883
AoxI GGCC 9 cut(s) 97, 304, 340, 793, 1189, 1422, 1572, 1637, 1817
ApeKI GCWGC 2 cut(s) 515, 1249
ApoI RAATTY 7 cut(s) 263, 401, 452, 898, 1163, 1207, 1448
Asp718I GGTACC 2 cut(s) 605, 1539
AspLEI GCGC 1 cut(s) 504
AspS9I GGNCC 5 cut(s) 858, 992, 1572, 1655, 1971
AsuC2I CCSGG 1 cut(s) 348
AsuHPI GGTGA 3 cut(s) 1193, 1327, 1865
AvaI CYCGRG 2 cut(s) 1535, 1883
AvaII GGWCC 4 cut(s) 858, 992, 1655, 1971
AxyI CCTNAGG 2 cut(s) 1311, 1960
BaeI ACNNNNGTAYC 2 cut(s) 1523, 1556
BalI TGGCCA 1 cut(s) 795
BanI GGYRCC 3 cut(s) 46, 605, 1539
BanII GRGCYC 1 cut(s) 355
BauI CACGAG 1 cut(s) 1579
BbsI GAAGAC 1 cut(s) 1947
Bbv12I GWGCWC 3 cut(s) 355, 1557, 1586
BbvI GCAGC 2 cut(s) 502, 1236
BccI CCATC 5 cut(s) 80, 284, 530, 596, 1436
BceAI ACGGC 2 cut(s) 84, 142
BcgI CGANNNNNNTGC 2 cut(s) 1577, 1611
BclI TGATCA 1 cut(s) 1303
BcnI CCSGG 1 cut(s) 348
BcoDI GTCTC 2 cut(s) 158, 391
BcuI ACTAGT 1 cut(s) 34
BfuAI ACCTGC 1 cut(s) 836
BisI GCNGC 5 cut(s) 304, 516, 1250, 1812, 1900
BlsI GCNGC 5 cut(s) 305, 517, 1251, 1813, 1901
BmcAI AGTACT 3 cut(s) 30, 545, 1844
Bme1390I CCNGG 1 cut(s) 348
Bme18I GGWCC 4 cut(s) 858, 992, 1655, 1971
BmeRI GACNNNNNGTC 1 cut(s) 1653
BmeT110I CYCGRG 2 cut(s) 1535, 1883
BmgBI CACGTC 1 cut(s) 1591
BmgT120I GGNCC 5 cut(s) 858, 992, 1572, 1655, 1971
BmiI GGNNCC 8 cut(s) 48, 607, 636, 1331, 1541, 1656, 1806, 1972
BmrFI CCNGG 1 cut(s) 348
BmrI ACTGGG 1 cut(s) 206
BmuI ACTGGG 1 cut(s) 206
BpiI GAAGAC 1 cut(s) 1947
Bpu10I CCTNAGC 1 cut(s) 371
BpuEI CTTGAG 2 cut(s) 786, 1736
BpuMI CCSGG 1 cut(s) 348
BsaBI GATNNNNATC 1 cut(s) 488
BsaI GGTCTC 1 cut(s) 158
BsaJI CCNNGG 3 cut(s) 307, 1495, 1755
BsaWI WCCGGW 1 cut(s) 1203
BsaXI ACNNNNNCTCC 6 cut(s) 619, 649, 971, 1001, 1757, 1787
Bsc4I CCNNNNNNNGG 4 cut(s) 338, 396, 1035, 1326
Bse118I RCCGGY 1 cut(s) 1191
Bse1I ACTGG 5 cut(s) 100, 212, 364, 745, 1425
Bse21I CCTNAGG 2 cut(s) 1311, 1960
Bse3DI GCAATG 1 cut(s) 840
Bse8I GATNNNNATC 1 cut(s) 488
BseDI CCNNGG 3 cut(s) 307, 1495, 1755
BseGI GGATG 5 cut(s) 276, 755, 792, 1161, 1710
BseJI GATNNNNATC 1 cut(s) 488
BseLI CCNNNNNNNGG 4 cut(s) 338, 396, 1035, 1326
BseMI GCAATG 1 cut(s) 840
BseMII CTCAG 6 cut(s) 130, 173, 243, 362, 1010, 1302
BseNI ACTGG 5 cut(s) 100, 212, 364, 745, 1425
BseRI GAGGAG 1 cut(s) 1093
BseXI GCAGC 2 cut(s) 502, 1236
BseYI CCCAGC 1 cut(s) 520
BshFI GGCC 9 cut(s) 99, 306, 342, 795, 1191, 1424, 1574, 1639, 1819
BshNI GGYRCC 3 cut(s) 46, 605, 1539
BsiHKAI GWGCWC 3 cut(s) 355, 1557, 1586
BsiHKCI CYCGRG 2 cut(s) 1535, 1883
BsiSI CCGG 4 cut(s) 348, 1192, 1204, 1868
BslI CCNNNNNNNGG 4 cut(s) 338, 396, 1035, 1326
BsmAI GTCTC 2 cut(s) 158, 391
BsmBI CGTCTC 1 cut(s) 391
BsmI GAATGC 1 cut(s) 1978
BsnI GGCC 9 cut(s) 99, 306, 342, 795, 1191, 1424, 1574, 1639, 1819
Bso31I GGTCTC 1 cut(s) 158
BsoBI CYCGRG 2 cut(s) 1535, 1883
Bsp1286I GDGCHC 3 cut(s) 355, 1557, 1586
Bsp143I GATC 3 cut(s) 138, 1303, 1726
Bsp19I CCATGG 2 cut(s) 307, 1495
BspACI CCGC 4 cut(s) 243, 303, 1811, 1899
BspANI GGCC 9 cut(s) 99, 306, 342, 795, 1191, 1424, 1574, 1639, 1819
BspCNI CTCAG 6 cut(s) 129, 174, 242, 363, 1009, 1303
BspHI TCATGA 1 cut(s) 1381
BspLI GGNNCC 8 cut(s) 48, 607, 636, 1331, 1541, 1656, 1806, 1972
BspMI ACCTGC 1 cut(s) 836
BspT107I GGYRCC 3 cut(s) 46, 605, 1539
BspTNI GGTCTC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 840
BsrFI RCCGGY 1 cut(s) 1191
BsrI ACTGG 5 cut(s) 100, 212, 364, 745, 1425
BssAI RCCGGY 1 cut(s) 1191
BssECI CCNNGG 3 cut(s) 307, 1495, 1755
BssMI GATC 3 cut(s) 138, 1303, 1726
BssSI CACGAG 1 cut(s) 1579
BssT1I CCWWGG 2 cut(s) 307, 1495
Bst2BI CACGAG 1 cut(s) 1579
Bst4CI ACNGT 5 cut(s) 569, 1020, 1221, 1909, 1919
Bst6I CTCTTC 3 cut(s) 739, 941, 1121
BstC8I GCNNGC 6 cut(s) 500, 1193, 1235, 1479, 1809, 1826
BstDEI CTNAG 7 cut(s) 116, 182, 229, 371, 996, 1311, 1960
BstDSI CCRYGG 2 cut(s) 307, 1495
BstEII GGTNACC 1 cut(s) 1315
BstF5I GGATG 5 cut(s) 276, 755, 792, 1161, 1710
BstHHI GCGC 1 cut(s) 504
BstKTI GATC 3 cut(s) 141, 1306, 1729
BstMAI GTCTC 2 cut(s) 158, 391
BstMBI GATC 3 cut(s) 138, 1303, 1726
BstMWI GCNNNNNNNGC 6 cut(s) 436, 521, 641, 1604, 1825, 2000
BstNSI RCATGY 2 cut(s) 1273, 1828
BstPI GGTNACC 1 cut(s) 1315
BstSCI CCNGG 1 cut(s) 346
BstV1I GCAGC 2 cut(s) 502, 1236
BstV2I GAAGAC 1 cut(s) 1947
Bsu36I CCTNAGG 2 cut(s) 1311, 1960
BsuRI GGCC 9 cut(s) 99, 306, 342, 795, 1191, 1424, 1574, 1639, 1819
BtgI CCRYGG 2 cut(s) 307, 1495
BtgZI GCGATG 1 cut(s) 310
BtrI CACGTC 1 cut(s) 1591
BtsCI GGATG 5 cut(s) 276, 755, 792, 1161, 1710
BtsI GCAGTG 1 cut(s) 1128
BtsIMutI CAGTG 4 cut(s) 93, 574, 900, 1128
BveI ACCTGC 1 cut(s) 836
Cac8I GCNNGC 6 cut(s) 500, 1193, 1235, 1479, 1809, 1826
CaiI CAGNNNCTG 1 cut(s) 370
CciI TCATGA 1 cut(s) 1381
CfoI GCGC 1 cut(s) 504
Cfr10I RCCGGY 1 cut(s) 1191
Cfr13I GGNCC 5 cut(s) 858, 992, 1572, 1655, 1971
DdeI CTNAG 7 cut(s) 116, 182, 229, 371, 996, 1311, 1960
DpnI GATC 3 cut(s) 140, 1305, 1728
DpnII GATC 3 cut(s) 138, 1303, 1726
DraI TTTAAA 1 cut(s) 1342
DriI GACNNNNNGTC 1 cut(s) 1653
EaeI YGGCCR 4 cut(s) 97, 304, 793, 1189
Eam1104I CTCTTC 3 cut(s) 739, 941, 1121
Eam1105I GACNNNNNGTC 1 cut(s) 1653
EarI CTCTTC 3 cut(s) 739, 941, 1121
Ecl136II GAGCTC 1 cut(s) 353
Eco130I CCWWGG 2 cut(s) 307, 1495
Eco147I AGGCCT 1 cut(s) 342
Eco24I GRGCYC 1 cut(s) 355
Eco31I GGTCTC 1 cut(s) 158
Eco47I GGWCC 4 cut(s) 858, 992, 1655, 1971
Eco53kI GAGCTC 1 cut(s) 353
Eco57I CTGAAG 3 cut(s) 1128, 1347, 1680
Eco81I CCTNAGG 2 cut(s) 1311, 1960
Eco88I CYCGRG 2 cut(s) 1535, 1883
Eco91I GGTNACC 1 cut(s) 1315
EcoICRI GAGCTC 1 cut(s) 353
EcoO109I RGGNCCY 2 cut(s) 1655, 1971
EcoO65I GGTNACC 1 cut(s) 1315
EcoRI GAATTC 2 cut(s) 452, 1448
EcoT14I CCWWGG 2 cut(s) 307, 1495
EcoT38I GRGCYC 1 cut(s) 355
ErhI CCWWGG 2 cut(s) 307, 1495
Esp3I CGTCTC 1 cut(s) 391
FalI AAGNNNNNCTT 4 cut(s) 1863, 1895, 1958, 1990
FbaI TGATCA 1 cut(s) 1303
Fnu4HI GCNGC 5 cut(s) 304, 516, 1250, 1812, 1900
FokI GGATG 5 cut(s) 263, 742, 799, 1168, 1697
FriOI GRGCYC 1 cut(s) 355
Fsp4HI GCNGC 5 cut(s) 304, 516, 1250, 1812, 1900
GlaI GCGC 1 cut(s) 503
GluI GCNGC 5 cut(s) 304, 516, 1250, 1812, 1900
GsaI CCCAGC 1 cut(s) 524
HaeIII GGCC 9 cut(s) 99, 306, 342, 795, 1191, 1424, 1574, 1639, 1819
HapII CCGG 4 cut(s) 348, 1192, 1204, 1868
HhaI GCGC 1 cut(s) 504
Hin6I GCGC 1 cut(s) 502
HinP1I GCGC 1 cut(s) 502
HindIII AAGCTT 4 cut(s) 11, 161, 1282, 1778
HinfI GANTC 5 cut(s) 312, 585, 1142, 1405, 1997
HpaII CCGG 4 cut(s) 348, 1192, 1204, 1868
HphI GGTGA 3 cut(s) 1193, 1327, 1865
Hpy166II GTNNAC 1 cut(s) 291
Hpy188I TCNGA 8 cut(s) 232, 286, 333, 888, 999, 1108, 1731, 1804
Hpy8I GTNNAC 1 cut(s) 291
Hpy99I CGWCG 2 cut(s) 337, 1592
HpyAV CCTTC 8 cut(s) 208, 229, 262, 353, 936, 1153, 1414, 1452
HpyCH4III ACNGT 5 cut(s) 569, 1020, 1221, 1909, 1919
HpyCH4IV ACGT 2 cut(s) 384, 1590
HpyCH4V TGCA 7 cut(s) 650, 845, 959, 1233, 1481, 1978, 1994
HpyF10VI GCNNNNNNNGC 6 cut(s) 436, 521, 641, 1604, 1825, 2000
HpyF3I CTNAG 7 cut(s) 116, 182, 229, 371, 996, 1311, 1960
HpySE526I ACGT 2 cut(s) 384, 1590
HspAI GCGC 1 cut(s) 502
KpnI GGTACC 2 cut(s) 609, 1543
KroI GCCGGC 1 cut(s) 1191
KroNI GCCGGC 1 cut(s) 1193
Ksp22I TGATCA 1 cut(s) 1303
Kzo9I GATC 3 cut(s) 138, 1303, 1726
Lsp1109I GCAGC 2 cut(s) 502, 1236
MaeII ACGT 2 cut(s) 384, 1590
MaeIII GTNAC 6 cut(s) 214, 625, 1199, 1315, 1706, 1875
MalI GATC 3 cut(s) 140, 1305, 1728
MboI GATC 3 cut(s) 138, 1303, 1726
MboII GAAGA 9 cut(s) 726, 958, 983, 1138, 1370, 1397, 1673, 1884, 1947
MfeI CAATTG 1 cut(s) 639
MhlI GDGCHC 3 cut(s) 355, 1557, 1586
MlsI TGGCCA 1 cut(s) 795
MluNI TGGCCA 1 cut(s) 795
MlyI GAGTC 2 cut(s) 594, 2006
MmeI TCCRAC 6 cut(s) 356, 414, 755, 1507, 1676, 1891
Mox20I TGGCCA 1 cut(s) 795
MroNI GCCGGC 1 cut(s) 1191
MscI TGGCCA 1 cut(s) 795
MseI TTAA 6 cut(s) 111, 564, 1005, 1323, 1341, 1904
MslI CAYNNNNRTG 2 cut(s) 1413, 1589
Msp20I TGGCCA 1 cut(s) 795
MspA1I CMGCKG 3 cut(s) 303, 524, 1811
MspI CCGG 4 cut(s) 348, 1192, 1204, 1868
MspR9I CCNGG 1 cut(s) 348
MunI CAATTG 1 cut(s) 639
Mva1269I GAATGC 1 cut(s) 1978
MwoI GCNNNNNNNGC 6 cut(s) 436, 521, 641, 1604, 1825, 2000
NaeI GCCGGC 1 cut(s) 1193
NciI CCSGG 1 cut(s) 348
NcoI CCATGG 2 cut(s) 307, 1495
NdeII GATC 3 cut(s) 138, 1303, 1726
NgoMIV GCCGGC 1 cut(s) 1191
NlaIV GGNNCC 8 cut(s) 48, 607, 636, 1331, 1541, 1656, 1806, 1972
NmeAIII GCCGAG 1 cut(s) 1780
NmuCI GTSAC 3 cut(s) 1199, 1315, 1875
NspI RCATGY 2 cut(s) 1273, 1828
OliI CACNNNNGTG 1 cut(s) 1589
PaeI GCATGC 1 cut(s) 1828
PagI TCATGA 1 cut(s) 1381
PceI AGGCCT 1 cut(s) 342
PctI GAATGC 1 cut(s) 1978
PdiI GCCGGC 1 cut(s) 1193
PfeI GAWTC 3 cut(s) 312, 1142, 1405
PfoI TCCNGGA 1 cut(s) 346
PkrI GCNGC 5 cut(s) 305, 517, 1251, 1813, 1901
PleI GAGTC 2 cut(s) 593, 2005
PpsI GAGTC 2 cut(s) 593, 2005
PpuMI RGGWCCY 2 cut(s) 1655, 1971
Psp124BI GAGCTC 1 cut(s) 355
Psp5II RGGWCCY 2 cut(s) 1655, 1971
PspEI GGTNACC 1 cut(s) 1315
PspFI CCCAGC 1 cut(s) 520
PspN4I GGNNCC 8 cut(s) 48, 607, 636, 1331, 1541, 1656, 1806, 1972
PspPI GGNCC 5 cut(s) 858, 992, 1572, 1655, 1971
PspPPI RGGWCCY 2 cut(s) 1655, 1971
PstNI CAGNNNCTG 1 cut(s) 370
PvuII CAGCTG 1 cut(s) 524
RseI CAYNNNNRTG 2 cut(s) 1413, 1589
SacI GAGCTC 1 cut(s) 355
SaqAI TTAA 6 cut(s) 111, 564, 1005, 1323, 1341, 1904
SatI GCNGC 5 cut(s) 304, 516, 1250, 1812, 1900
Sau3AI GATC 3 cut(s) 138, 1303, 1726
Sau96I GGNCC 5 cut(s) 858, 992, 1572, 1655, 1971
ScaI AGTACT 3 cut(s) 30, 545, 1844
SchI GAGTC 2 cut(s) 594, 2006
ScrFI CCNGG 1 cut(s) 348
SduI GDGCHC 3 cut(s) 355, 1557, 1586
SinI GGWCC 4 cut(s) 858, 992, 1655, 1971
SmiMI CAYNNNNRTG 2 cut(s) 1413, 1589
SmlI CTYRAG 2 cut(s) 801, 1715
SmoI CTYRAG 2 cut(s) 801, 1715
SpeI ACTAGT 1 cut(s) 34
SphI GCATGC 1 cut(s) 1828
SseBI AGGCCT 1 cut(s) 342
SsiI CCGC 4 cut(s) 243, 303, 1811, 1899
SstI GAGCTC 1 cut(s) 355
StuI AGGCCT 1 cut(s) 342
StyD4I CCNGG 1 cut(s) 346
StyI CCWWGG 2 cut(s) 307, 1495
TaaI ACNGT 5 cut(s) 569, 1020, 1221, 1909, 1919
TaiI ACGT 2 cut(s) 387, 1593
TaqI TCGA 2 cut(s) 359, 1617
TatI WGTACW 6 cut(s) 28, 288, 543, 1008, 1213, 1842
TauI GCSGC 3 cut(s) 306, 1814, 1902
TfiI GAWTC 3 cut(s) 312, 1142, 1405
Tru1I TTAA 6 cut(s) 111, 564, 1005, 1323, 1341, 1904
Tru9I TTAA 6 cut(s) 111, 564, 1005, 1323, 1341, 1904
TscAI CASTG 4 cut(s) 100, 574, 900, 1128
TseFI GTSAC 3 cut(s) 1199, 1315, 1875
TseI GCWGC 2 cut(s) 515, 1249
Tsp45I GTSAC 3 cut(s) 1199, 1315, 1875
TspDTI ATGAA 9 cut(s) 64, 483, 803, 1155, 1172, 1370, 1397, 1398, 1971
TspGWI ACGGA 2 cut(s) 464, 1757
TspRI CASTG 4 cut(s) 100, 574, 900, 1128
VpaK11BI GGWCC 4 cut(s) 858, 992, 1655, 1971
XapI RAATTY 7 cut(s) 263, 401, 452, 898, 1163, 1207, 1448
XbaI TCTAGA 1 cut(s) 121
XceI RCATGY 2 cut(s) 1273, 1828
ZrmI AGTACT 3 cut(s) 30, 545, 1844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.