AT5G03890

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004049
Physical Location & Seq
Forward (+)
1046981 .. 1047955
975 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G03890.1

Sequence Viewer

Length: 540 bp
ATGGGGAACTGTTTAGTAATGGAGAAGAAAGTGATAAAGATAGTGAGAGATGATGGTAAGGTACTTGAATATAGAGAACCAATTAGTGTTCATCACATCCTTACTCAATTCTCTGGTCACTCCATCTCTCACAACAACACTCATCTCCTGCCTGATGCTAAGCTTCTCTCTGGCCGTCTCTATTATCTCCTGCCTACAACAATGACCAAGAAGAAGGTCAACAAGAAAGTGACCTTTGCGAATCCGGAGGTGGAAGGCGACGAAAGATTACTTAGAGAAGAAGAAGATTCTAGTGAAAGCAACAGCAATATCGATGGTGATGACACCAAGAACGTAACTGTTGTAAGAATGAAGATCGTTGTGCATAAGCAAGAGTTGGAGAAGCTACTTCAAGGAGGGTCGGTCCATGAGATGATGTACCAGACTCTTGAGAAACAACTTTTACTTACTTCGTCTGATGATGATGATCTTGAATGTAATAGCGGCTGGAGACCTGCGTTAGATAGCATACCCGAATCTGAAAGTCTCAGAAGAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

179

Amino Acids

20.39

Weight (kDa)

5.66

Isoelectric Point (pI)

48.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 172 1.2e-25 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 502
AccIII TCCGGA 1 cut(s) 244
AciI CCGC 1 cut(s) 483
AcoI YGGCCR 1 cut(s) 172
AfaI GTAC 2 cut(s) 63, 419
AgsI TTSAA 3 cut(s) 68, 392, 473
AluBI AGCT 2 cut(s) 163, 385
AluI AGCT 2 cut(s) 163, 385
Alw26I GTCTC 3 cut(s) 182, 484, 530
Aor13HI TCCGGA 1 cut(s) 244
AoxI GGCC 1 cut(s) 172
AspS9I GGNCC 1 cut(s) 403
AsuHPI GGTGA 1 cut(s) 329
AvaII GGWCC 1 cut(s) 403
BccI CCATC 3 cut(s) 47, 131, 308
BceAI ACGGC 1 cut(s) 159
BcoDI GTCTC 3 cut(s) 182, 484, 530
BfaI CTAG 1 cut(s) 291
BfuAI ACCTGC 1 cut(s) 502
BisI GCNGC 1 cut(s) 484
BlpI GCTNAGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 485
Bme18I GGWCC 1 cut(s) 403
BmgT120I GGNCC 1 cut(s) 403
BmsI GCATC 1 cut(s) 145
BpmI CTGGAG 1 cut(s) 508
Bpu1102I GCTNAGC 1 cut(s) 159
BpuEI CTTGAG 1 cut(s) 449
Bsa29I ATCGAT 1 cut(s) 312
BsaBI GATNNNNATC 1 cut(s) 465
BsaI GGTCTC 1 cut(s) 484
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 2 cut(s) 387, 417
Bse8I GATNNNNATC 1 cut(s) 465
BseAI TCCGGA 1 cut(s) 244
BseCI ATCGAT 1 cut(s) 312
BseGI GGATG 1 cut(s) 96
BseJI GATNNNNATC 1 cut(s) 465
BshFI GGCC 1 cut(s) 174
BshVI ATCGAT 1 cut(s) 312
BsiSI CCGG 1 cut(s) 245
BsmAI GTCTC 3 cut(s) 182, 484, 530
BsmBI CGTCTC 1 cut(s) 182
BsnI GGCC 1 cut(s) 174
Bso31I GGTCTC 1 cut(s) 484
Bsp13I TCCGGA 1 cut(s) 244
Bsp143I GATC 2 cut(s) 354, 466
Bsp1720I GCTNAGC 1 cut(s) 159
BspACI CCGC 1 cut(s) 483
BspANI GGCC 1 cut(s) 174
BspCNI CTCAG 1 cut(s) 540
BspDI ATCGAT 1 cut(s) 312
BspEI TCCGGA 1 cut(s) 244
BspMI ACCTGC 1 cut(s) 502
BspTNI GGTCTC 1 cut(s) 484
BssMI GATC 2 cut(s) 354, 466
Bst4CI ACNGT 2 cut(s) 11, 340
BstDEI CTNAG 3 cut(s) 159, 272, 527
BstF5I GGATG 1 cut(s) 96
BstKTI GATC 2 cut(s) 357, 469
BstMAI GTCTC 3 cut(s) 182, 484, 530
BstMBI GATC 2 cut(s) 354, 466
Bsu15I ATCGAT 1 cut(s) 312
BsuRI GGCC 1 cut(s) 174
BsuTUI ATCGAT 1 cut(s) 312
BtsCI GGATG 1 cut(s) 96
BveI ACCTGC 1 cut(s) 502
Cfr13I GGNCC 1 cut(s) 403
ClaI ATCGAT 1 cut(s) 312
Csp6I GTAC 2 cut(s) 62, 418
CviAII CATG 1 cut(s) 407
CviJI RGCY 4 cut(s) 163, 174, 385, 486
CviKI_1 RGCY 4 cut(s) 163, 174, 385, 486
CviQI GTAC 2 cut(s) 62, 418
DdeI CTNAG 3 cut(s) 159, 272, 527
DpnI GATC 2 cut(s) 356, 468
DpnII GATC 2 cut(s) 354, 466
EaeI YGGCCR 1 cut(s) 172
Eco31I GGTCTC 1 cut(s) 484
Eco47I GGWCC 1 cut(s) 403
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 1 cut(s) 410
FaiI YATR 5 cut(s) 72, 366, 408, 509, 538
FatI CATG 1 cut(s) 406
Fnu4HI GCNGC 1 cut(s) 484
FokI GGATG 1 cut(s) 83
Fsp4HI GCNGC 1 cut(s) 484
FspBI CTAG 1 cut(s) 291
GluI GCNGC 1 cut(s) 484
GsuI CTGGAG 1 cut(s) 508
HaeIII GGCC 1 cut(s) 174
HapII CCGG 1 cut(s) 245
Hin1II CATG 1 cut(s) 410
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HindIII AAGCTT 1 cut(s) 161
HinfI GANTC 4 cut(s) 241, 287, 424, 515
HpaII CCGG 1 cut(s) 245
HphI GGTGA 1 cut(s) 329
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 3 cut(s) 457, 520, 530
Hpy188III TCNNGA 3 cut(s) 245, 428, 470
Hpy8I GTNNAC 1 cut(s) 220
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 2 cut(s) 208, 248
HpyCH4III ACNGT 2 cut(s) 11, 340
HpyCH4IV ACGT 1 cut(s) 333
HpyCH4V TGCA 1 cut(s) 364
HpyF3I CTNAG 3 cut(s) 159, 272, 527
HpySE526I ACGT 1 cut(s) 333
Hsp92II CATG 1 cut(s) 410
Kpn2I TCCGGA 1 cut(s) 244
Kzo9I GATC 2 cut(s) 354, 466
LpnPI CCDG 9 cut(s) 99, 156, 161, 165, 203, 258, 434, 472, 507
LweI GCATC 1 cut(s) 145
MaeI CTAG 1 cut(s) 291
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 3 cut(s) 116, 229, 334
MalI GATC 2 cut(s) 356, 468
MboI GATC 2 cut(s) 354, 466
MboII GAAGA 6 cut(s) 37, 223, 290, 293, 296, 364
MluCI AATT 2 cut(s) 81, 107
MlyI GAGTC 1 cut(s) 418
MmeI TCCRAC 1 cut(s) 357
MnlI CCTC 2 cut(s) 241, 389
MroI TCCGGA 1 cut(s) 244
MspI CCGG 1 cut(s) 245
NdeII GATC 2 cut(s) 354, 466
NlaIII CATG 1 cut(s) 410
NmuCI GTSAC 2 cut(s) 116, 229
PfeI GAWTC 3 cut(s) 241, 287, 515
PkrI GCNGC 1 cut(s) 485
PleI GAGTC 1 cut(s) 418
PpsI GAGTC 1 cut(s) 418
PspPI GGNCC 1 cut(s) 403
RsaI GTAC 2 cut(s) 63, 419
RsaNI GTAC 2 cut(s) 62, 418
SatI GCNGC 1 cut(s) 484
Sau3AI GATC 2 cut(s) 354, 466
Sau96I GGNCC 1 cut(s) 403
SchI GAGTC 1 cut(s) 418
SetI ASST 8 cut(s) 63, 165, 219, 236, 252, 336, 387, 496
SfaNI GCATC 1 cut(s) 145
SinI GGWCC 1 cut(s) 403
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 2 cut(s) 81, 107
SsiI CCGC 1 cut(s) 483
SspMI CTAG 1 cut(s) 291
TaaI ACNGT 2 cut(s) 11, 340
TaiI ACGT 1 cut(s) 336
TaqI TCGA 1 cut(s) 312
TaqII GACCGA 1 cut(s) 391
TasI AATT 2 cut(s) 81, 107
TauI GCSGC 1 cut(s) 486
TfiI GAWTC 3 cut(s) 241, 287, 515
TseFI GTSAC 2 cut(s) 116, 229
Tsp45I GTSAC 2 cut(s) 116, 229
TspDTI ATGAA 2 cut(s) 80, 365
VpaK11BI GGWCC 1 cut(s) 403
XspI CTAG 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.