Rw3G017030

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0004049
Physical Location & Seq
Reverse (-)
18354103 .. 18354746
644 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G017030.1

Sequence Viewer

Length: 564 bp
ATGGGGTCCAGAGACAAACAAGGTATATCTGTAATATTGATGAAGTATGAGGGGAAGGTTGTAACCATCTACTTTAAATACCACTCTTATTCCCACCACCCTCATTCCTTCACAACTTTTAAAACTAAGTTTTATGGGAAAGTCCTAGAGTACAGAGCATCCATAAAAGTTTACCAGGTGTTGTCCGAGTTTTCTGGCCACGCAATATCTGAGGCGACTCCGGTGTACCAGCATCTCCGGCCAGATACAAAGCTGCTCGGTGGTCGCCTGTACTATCTTGTACCTCTGCCTCTGCCGTCAGAGAAGGCATCACAGAAGAAGGTGAGGTTTTCAACTGATCCTGAAGGTGAAGCTCAACAAGAAACTAAAGTTGTAAGAATTAAGCTAGTTATAAGCAAGAAACAACTGCAGGAGATGTTGGGAAAGGGAGGGGTTTCAGTTGATGACATGGTTTCCAGAATTCAATGTCAAACAAGCACAGATAAGAAGACTGACAGCTCCGACAACGATGGTAGTTGTGAAGGGTGGAAACCAGTTTTAGAAAGCATACCTGAAGTAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

187

Amino Acids

21.14

Weight (kDa)

9.16

Isoelectric Point (pI)

28.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 46 - 185 3.3e-16 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 392
AclWI GGATC 1 cut(s) 332
AcoI YGGCCR 2 cut(s) 196, 239
AcsI RAATTY 1 cut(s) 459
AcuI CTGAAG 1 cut(s) 363
AfaI GTAC 4 cut(s) 152, 227, 272, 282
AgsI TTSAA 2 cut(s) 333, 464
AjnI CCWGG 1 cut(s) 174
AluBI AGCT 4 cut(s) 253, 353, 385, 498
AluI AGCT 4 cut(s) 253, 353, 385, 498
Alw26I GTCTC 1 cut(s) 6
AlwI GGATC 1 cut(s) 332
AoxI GGCC 2 cut(s) 196, 239
ApeKI GCWGC 1 cut(s) 253
ApoI RAATTY 1 cut(s) 459
AspS9I GGNCC 1 cut(s) 6
AsuHPI GGTGA 2 cut(s) 334, 359
AvaII GGWCC 1 cut(s) 6
BalI TGGCCA 1 cut(s) 198
BbsI GAAGAC 1 cut(s) 494
BbvI GCAGC 1 cut(s) 240
BccI CCATC 2 cut(s) 74, 503
BceAI ACGGC 1 cut(s) 280
BciT130I CCWGG 1 cut(s) 176
BcoDI GTCTC 1 cut(s) 6
BfaI CTAG 3 cut(s) 146, 386, 562
BfmI CTRYAG 1 cut(s) 407
BisI GCNGC 1 cut(s) 254
BlsI GCNGC 1 cut(s) 255
Bme1390I CCNGG 1 cut(s) 176
Bme18I GGWCC 1 cut(s) 6
BmgT120I GGNCC 1 cut(s) 6
BmiI GGNNCC 1 cut(s) 7
BmrFI CCNGG 1 cut(s) 176
BmsI GCATC 3 cut(s) 167, 241, 317
BpiI GAAGAC 1 cut(s) 494
BsaWI WCCGGW 1 cut(s) 220
Bse1I ACTGG 1 cut(s) 533
BseBI CCWGG 1 cut(s) 176
BseGI GGATG 1 cut(s) 158
BseMII CTCAG 1 cut(s) 201
BseNI ACTGG 1 cut(s) 533
BseXI GCAGC 1 cut(s) 240
BshFI GGCC 2 cut(s) 198, 241
BsiSI CCGG 2 cut(s) 221, 238
BsmAI GTCTC 1 cut(s) 6
BsnI GGCC 2 cut(s) 198, 241
Bsp143I GATC 1 cut(s) 337
BspANI GGCC 2 cut(s) 198, 241
BspCNI CTCAG 1 cut(s) 202
BspLI GGNNCC 1 cut(s) 7
BspMAI CTGCAG 1 cut(s) 411
BspPI GGATC 1 cut(s) 332
BsrI ACTGG 1 cut(s) 533
BssMI GATC 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 176
BstDEI CTNAG 2 cut(s) 126, 210
BstF5I GGATG 1 cut(s) 158
BstKTI GATC 1 cut(s) 340
BstMAI GTCTC 1 cut(s) 6
BstMBI GATC 1 cut(s) 337
BstMWI GCNNNNNNNGC 1 cut(s) 238
BstNI CCWGG 1 cut(s) 176
BstSCI CCNGG 1 cut(s) 174
BstSFI CTRYAG 1 cut(s) 407
BstV1I GCAGC 1 cut(s) 240
BstV2I GAAGAC 1 cut(s) 494
BsuRI GGCC 2 cut(s) 198, 241
BtsCI GGATG 1 cut(s) 158
Cfr13I GGNCC 1 cut(s) 6
CsiI ACCWGGT 1 cut(s) 174
Csp6I GTAC 4 cut(s) 151, 226, 271, 281
CviAII CATG 1 cut(s) 448
CviJI RGCY 6 cut(s) 198, 241, 253, 353, 385, 498
CviKI_1 RGCY 6 cut(s) 198, 241, 253, 353, 385, 498
CviQI GTAC 4 cut(s) 151, 226, 271, 281
DdeI CTNAG 2 cut(s) 126, 210
DpnI GATC 1 cut(s) 339
DpnII GATC 1 cut(s) 337
DraI TTTAAA 2 cut(s) 76, 121
EaeI YGGCCR 2 cut(s) 196, 239
Eco47I GGWCC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 363
EcoRI GAATTC 1 cut(s) 459
EcoRII CCWGG 1 cut(s) 174
FaeI CATG 1 cut(s) 451
FaiI YATR 7 cut(s) 26, 48, 135, 164, 392, 449, 548
FatI CATG 1 cut(s) 447
Fnu4HI GCNGC 1 cut(s) 254
FokI GGATG 1 cut(s) 145
Fsp4HI GCNGC 1 cut(s) 254
FspBI CTAG 3 cut(s) 146, 386, 562
GluI GCNGC 1 cut(s) 254
HaeIII GGCC 2 cut(s) 198, 241
HapII CCGG 2 cut(s) 221, 238
Hin1II CATG 1 cut(s) 451
HinfI GANTC 1 cut(s) 217
HpaII CCGG 2 cut(s) 221, 238
HphI GGTGA 2 cut(s) 334, 359
Hpy166II GTNNAC 3 cut(s) 172, 226, 559
Hpy188I TCNGA 4 cut(s) 187, 211, 301, 502
Hpy188III TCNNGA 3 cut(s) 9, 341, 456
Hpy8I GTNNAC 3 cut(s) 172, 226, 559
HpyAV CCTTC 6 cut(s) 49, 118, 298, 313, 338, 515
HpyCH4V TGCA 1 cut(s) 409
HpyF10VI GCNNNNNNNGC 1 cut(s) 238
HpyF3I CTNAG 2 cut(s) 126, 210
Hsp92II CATG 1 cut(s) 451
Kzo9I GATC 1 cut(s) 337
LmnI GCTCC 1 cut(s) 503
Lsp1109I GCAGC 1 cut(s) 240
LweI GCATC 3 cut(s) 167, 241, 317
MabI ACCWGGT 1 cut(s) 174
MaeI CTAG 3 cut(s) 146, 386, 562
MaeIII GTNAC 1 cut(s) 61
MalI GATC 1 cut(s) 339
MboI GATC 1 cut(s) 337
MboII GAAGA 2 cut(s) 328, 499
MlsI TGGCCA 1 cut(s) 198
MluCI AATT 2 cut(s) 378, 459
MluNI TGGCCA 1 cut(s) 198
MlyI GAGTC 1 cut(s) 211
MmeI TCCRAC 1 cut(s) 525
MnlI CCTC 7 cut(s) 43, 111, 205, 294, 300, 318, 422
Mox20I TGGCCA 1 cut(s) 198
MscI TGGCCA 1 cut(s) 198
MseI TTAA 3 cut(s) 75, 120, 381
Msp20I TGGCCA 1 cut(s) 198
MspI CCGG 2 cut(s) 221, 238
MspR9I CCNGG 1 cut(s) 176
MvaI CCWGG 1 cut(s) 176
MwoI GCNNNNNNNGC 1 cut(s) 238
NdeII GATC 1 cut(s) 337
NlaIII CATG 1 cut(s) 451
NlaIV GGNNCC 1 cut(s) 7
PkrI GCNGC 1 cut(s) 255
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
PsiI TTATAA 1 cut(s) 392
Psp6I CCWGG 1 cut(s) 174
PspGI CCWGG 1 cut(s) 174
PspN4I GGNNCC 1 cut(s) 7
PspPI GGNCC 1 cut(s) 6
PstI CTGCAG 1 cut(s) 411
RsaI GTAC 4 cut(s) 152, 227, 272, 282
RsaNI GTAC 4 cut(s) 151, 226, 271, 281
SaqAI TTAA 3 cut(s) 75, 120, 381
SatI GCNGC 1 cut(s) 254
Sau3AI GATC 1 cut(s) 337
Sau96I GGNCC 1 cut(s) 6
SchI GAGTC 1 cut(s) 211
ScrFI CCNGG 1 cut(s) 176
SexAI ACCWGGT 1 cut(s) 174
SfaNI GCATC 3 cut(s) 167, 241, 317
SfcI CTRYAG 1 cut(s) 407
SinI GGWCC 1 cut(s) 6
Sse9I AATT 2 cut(s) 378, 459
SspI AATATT 1 cut(s) 36
SspMI CTAG 3 cut(s) 146, 386, 562
StyD4I CCNGG 1 cut(s) 174
TasI AATT 2 cut(s) 378, 459
TatI WGTACW 2 cut(s) 150, 270
Tru1I TTAA 3 cut(s) 75, 120, 381
Tru9I TTAA 3 cut(s) 75, 120, 381
TseI GCWGC 1 cut(s) 253
TspDTI ATGAA 1 cut(s) 56
VpaK11BI GGWCC 1 cut(s) 6
XapI RAATTY 1 cut(s) 459
XspI CTAG 3 cut(s) 146, 386, 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.