Rroxscaffold_6G00407770

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004049
Physical Location & Seq
Forward (+)
30413391 .. 30415875
2485 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_6G00407770.1

Sequence Viewer

Length: 675 bp
ATGGGGTCCAGAGACAAACAAGGTATGTCTGTAATATTGATGAAGTATGAGGGGAAGGTTGTAACCATCTACTTTAAATACCACTCTTATTCCCATCACCCTCATTCCTTCACAACTTTTAAAACTAAAGTTTTGTCACCAAGCTTTGATAAAACAGGAGCAATGGGAAATTGTTTGGTTCTGCAACAAAATGTTACAAAGATAATGAAACCAGATGGGAAAGTCCTAGAGTACGGAGCATCCATAAAAGTTTACCAGGTGTTGTCCGAGTTTTCTGGCCACGCAATATCTGAGACGACTCCGGTGTACCAGCATCTCCGGCCAGATACAAAGCTGCTCGGTGGTCGACTGTACTATCTTGTACCTCTTCCTTTGCCGTCAGAGAAGGCATCTCAGAAGAAGGTGAGGTTTTCAACTGATCCTGAAGGTGAAGCTCAACAAGAAACTAAAGTTGTAAGAATTAAGCTAGTTATAAGCAAGCAACAACTGCAGGAGATGTTGGGAAAGGGAGGAGTTTCAGTTGATGACATGGTTTCCAGAATTCAATGTCAAACAAGCACAGATAAGAAGACTGACAGCTTCGACAACGATGGTAGTTGTGAAGGGTGGAAACCAGTTTTAGAAAGCATACCTGAAATGCCATTAGTAAGTTTGGCTTCCTATAGAAAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

224

Amino Acids

25.1

Weight (kDa)

9.06

Isoelectric Point (pI)

31.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 55 - 212 1.9e-21 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 473
AccI GTMKAC 1 cut(s) 346
AclWI GGATC 1 cut(s) 413
AcoI YGGCCR 2 cut(s) 277, 320
AcsI RAATTY 1 cut(s) 540
AcuI CTGAAG 1 cut(s) 444
AfaI GTAC 4 cut(s) 233, 308, 353, 363
AgsI TTSAA 2 cut(s) 414, 545
AjnI CCWGG 1 cut(s) 255
AluBI AGCT 5 cut(s) 144, 334, 434, 466, 579
AluI AGCT 5 cut(s) 144, 334, 434, 466, 579
Alw26I GTCTC 2 cut(s) 6, 287
AlwI GGATC 1 cut(s) 413
AoxI GGCC 2 cut(s) 277, 320
ApeKI GCWGC 1 cut(s) 334
ApoI RAATTY 1 cut(s) 540
AspS9I GGNCC 1 cut(s) 6
AsuHPI GGTGA 4 cut(s) 89, 129, 415, 440
AvaII GGWCC 1 cut(s) 6
BalI TGGCCA 1 cut(s) 279
BbsI GAAGAC 1 cut(s) 575
BbvI GCAGC 1 cut(s) 321
BccI CCATC 4 cut(s) 74, 102, 209, 584
BceAI ACGGC 1 cut(s) 361
BciT130I CCWGG 1 cut(s) 257
BcoDI GTCTC 2 cut(s) 6, 287
BfaI CTAG 3 cut(s) 227, 467, 673
BfmI CTRYAG 2 cut(s) 488, 661
BisI GCNGC 1 cut(s) 335
BlsI GCNGC 1 cut(s) 336
Bme1390I CCNGG 1 cut(s) 257
Bme18I GGWCC 1 cut(s) 6
BmgT120I GGNCC 1 cut(s) 6
BmiI GGNNCC 1 cut(s) 7
BmrFI CCNGG 1 cut(s) 257
BmsI GCATC 3 cut(s) 248, 322, 398
BpiI GAAGAC 1 cut(s) 575
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 2 cut(s) 504, 534
Bse1I ACTGG 1 cut(s) 614
Bse3DI GCAATG 1 cut(s) 168
BseBI CCWGG 1 cut(s) 257
BseGI GGATG 1 cut(s) 239
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 2 cut(s) 282, 407
BseNI ACTGG 1 cut(s) 614
BseRI GAGGAG 1 cut(s) 525
BseXI GCAGC 1 cut(s) 321
BshFI GGCC 2 cut(s) 279, 322
BsiSI CCGG 2 cut(s) 302, 319
BsmAI GTCTC 2 cut(s) 6, 287
BsmBI CGTCTC 1 cut(s) 287
BsnI GGCC 2 cut(s) 279, 322
Bsp143I GATC 1 cut(s) 418
BspANI GGCC 2 cut(s) 279, 322
BspCNI CTCAG 2 cut(s) 283, 406
BspLI GGNNCC 1 cut(s) 7
BspMAI CTGCAG 1 cut(s) 492
BspPI GGATC 1 cut(s) 413
BsrDI GCAATG 1 cut(s) 168
BsrI ACTGG 1 cut(s) 614
BssMI GATC 1 cut(s) 418
Bst2UI CCWGG 1 cut(s) 257
Bst4CI ACNGT 1 cut(s) 351
Bst6I CTCTTC 1 cut(s) 372
BstAPI GCANNNNNTGC 1 cut(s) 487
BstC8I GCNNGC 1 cut(s) 479
BstDEI CTNAG 2 cut(s) 291, 393
BstF5I GGATG 1 cut(s) 239
BstKTI GATC 1 cut(s) 421
BstMAI GTCTC 2 cut(s) 6, 287
BstMBI GATC 1 cut(s) 418
BstMWI GCNNNNNNNGC 2 cut(s) 319, 487
BstNI CCWGG 1 cut(s) 257
BstSCI CCNGG 1 cut(s) 255
BstSFI CTRYAG 2 cut(s) 488, 661
BstV1I GCAGC 1 cut(s) 321
BstV2I GAAGAC 1 cut(s) 575
BsuRI GGCC 2 cut(s) 279, 322
BtsCI GGATG 1 cut(s) 239
Cac8I GCNNGC 1 cut(s) 479
Cfr13I GGNCC 1 cut(s) 6
CsiI ACCWGGT 1 cut(s) 255
Csp6I GTAC 4 cut(s) 232, 307, 352, 362
CviAII CATG 1 cut(s) 529
CviJI RGCY 8 cut(s) 144, 279, 322, 334, 434, 466, 579, 656
CviKI_1 RGCY 8 cut(s) 144, 279, 322, 334, 434, 466, 579, 656
CviQI GTAC 4 cut(s) 232, 307, 352, 362
DdeI CTNAG 2 cut(s) 291, 393
DpnI GATC 1 cut(s) 420
DpnII GATC 1 cut(s) 418
DraI TTTAAA 2 cut(s) 76, 121
EaeI YGGCCR 2 cut(s) 277, 320
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
Eco47I GGWCC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 444
EcoRI GAATTC 1 cut(s) 540
EcoRII CCWGG 1 cut(s) 255
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 1 cut(s) 532
FaiI YATR 7 cut(s) 26, 48, 245, 473, 530, 629, 663
FalI AAGNNNNNCTT 2 cut(s) 640, 672
FatI CATG 1 cut(s) 528
FblI GTMKAC 1 cut(s) 346
Fnu4HI GCNGC 1 cut(s) 335
FokI GGATG 1 cut(s) 226
Fsp4HI GCNGC 1 cut(s) 335
FspBI CTAG 3 cut(s) 227, 467, 673
GluI GCNGC 1 cut(s) 335
HaeIII GGCC 2 cut(s) 279, 322
HapII CCGG 2 cut(s) 302, 319
Hin1II CATG 1 cut(s) 532
HincII GTYRAC 1 cut(s) 347
HindII GTYRAC 1 cut(s) 347
HindIII AAGCTT 1 cut(s) 142
HinfI GANTC 1 cut(s) 298
HpaII CCGG 2 cut(s) 302, 319
HphI GGTGA 4 cut(s) 89, 129, 415, 440
Hpy166II GTNNAC 3 cut(s) 253, 307, 347
Hpy188I TCNGA 4 cut(s) 268, 292, 382, 396
Hpy188III TCNNGA 3 cut(s) 9, 422, 537
Hpy8I GTNNAC 3 cut(s) 253, 307, 347
HpyAV CCTTC 6 cut(s) 49, 118, 379, 394, 419, 596
HpyCH4III ACNGT 1 cut(s) 351
HpyCH4V TGCA 2 cut(s) 184, 490
HpyF10VI GCNNNNNNNGC 2 cut(s) 319, 487
HpyF3I CTNAG 2 cut(s) 291, 393
Hsp92II CATG 1 cut(s) 532
Kzo9I GATC 1 cut(s) 418
LmnI GCTCC 2 cut(s) 158, 236
Lsp1109I GCAGC 1 cut(s) 321
LweI GCATC 3 cut(s) 248, 322, 398
MabI ACCWGGT 1 cut(s) 255
MaeI CTAG 3 cut(s) 227, 467, 673
MaeIII GTNAC 3 cut(s) 61, 135, 193
MalI GATC 1 cut(s) 420
MboI GATC 1 cut(s) 418
MboII GAAGA 3 cut(s) 359, 409, 580
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 3 cut(s) 169, 459, 540
MluNI TGGCCA 1 cut(s) 279
MlyI GAGTC 1 cut(s) 292
MnlI CCTC 5 cut(s) 43, 111, 375, 399, 503
Mox20I TGGCCA 1 cut(s) 279
MscI TGGCCA 1 cut(s) 279
MseI TTAA 3 cut(s) 75, 120, 462
Msp20I TGGCCA 1 cut(s) 279
MspI CCGG 2 cut(s) 302, 319
MspR9I CCNGG 1 cut(s) 257
MvaI CCWGG 1 cut(s) 257
MwoI GCNNNNNNNGC 2 cut(s) 319, 487
NdeII GATC 1 cut(s) 418
NlaIII CATG 1 cut(s) 532
NlaIV GGNNCC 1 cut(s) 7
NmuCI GTSAC 1 cut(s) 135
PkrI GCNGC 1 cut(s) 336
PleI GAGTC 1 cut(s) 292
PpsI GAGTC 1 cut(s) 292
PsiI TTATAA 1 cut(s) 473
Psp6I CCWGG 1 cut(s) 255
PspGI CCWGG 1 cut(s) 255
PspN4I GGNNCC 1 cut(s) 7
PspPI GGNCC 1 cut(s) 6
PstI CTGCAG 1 cut(s) 492
RsaI GTAC 4 cut(s) 233, 308, 353, 363
RsaNI GTAC 4 cut(s) 232, 307, 352, 362
SalI GTCGAC 1 cut(s) 345
SaqAI TTAA 3 cut(s) 75, 120, 462
SatI GCNGC 1 cut(s) 335
Sau3AI GATC 1 cut(s) 418
Sau96I GGNCC 1 cut(s) 6
SchI GAGTC 1 cut(s) 292
ScrFI CCNGG 1 cut(s) 257
SexAI ACCWGGT 1 cut(s) 255
SfaNI GCATC 3 cut(s) 248, 322, 398
SfcI CTRYAG 2 cut(s) 488, 661
SinI GGWCC 1 cut(s) 6
Sse9I AATT 3 cut(s) 169, 459, 540
SspI AATATT 1 cut(s) 36
SspMI CTAG 3 cut(s) 227, 467, 673
StyD4I CCNGG 1 cut(s) 255
TaaI ACNGT 1 cut(s) 351
TaqI TCGA 2 cut(s) 346, 582
TasI AATT 3 cut(s) 169, 459, 540
TatI WGTACW 1 cut(s) 351
Tru1I TTAA 3 cut(s) 75, 120, 462
Tru9I TTAA 3 cut(s) 75, 120, 462
TseFI GTSAC 1 cut(s) 135
TseI GCWGC 1 cut(s) 334
Tsp45I GTSAC 1 cut(s) 135
TspDTI ATGAA 2 cut(s) 56, 221
TspGWI ACGGA 1 cut(s) 249
VpaK11BI GGWCC 1 cut(s) 6
XapI RAATTY 1 cut(s) 540
XmiI GTMKAC 1 cut(s) 346
XspI CTAG 3 cut(s) 227, 467, 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.