MD12G1036700.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004049
Physical Location & Seq
Reverse (-)
3931653 .. 3932144
492 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1036700.v1.1.491

Sequence Viewer

Length: 492 bp
ATGGGGAATTGCTTGGTTATGCAAGAAAATATAACAAAGGTTATGAGACCTGATGGGAAAATCTTAGAGTACAGAGCAGACATGAAAGTTTACCAGGTGTTATCGGAGTTTTCCGGCCATGCAATATCTGAGACGGCTCCAGTCTACCAGCATCTCCGGCCAGACTCCAAGTTGCTTGGTGGTCACCTCTACTATCTCGTACCTCTCCCTCTGCCACCGCCGAAAAAGGCTTCCCAGAAGAAGGTGAGGTTTTCGGAGCCTGCAGAAGCCGAACCTGAACAAGAAACAAAAGTCGTAAGGATTAAGCTGGTTATTAGAAAGCAAGAGTTGCAGGAGATGTTGATAAAGGGAGGAGTTGCGGTTCTTGATGAAATGGTCTCTCGGCTTCAAAGTGAAAAAGGCGTAGAGAAGAGTAATACTAGCAGCTTCAACGATGATGGTAACTGTGAAGGGTGGAAACCTGTTCTACAAAGCATACCTGAACTAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

164

Amino Acids

18.36

Weight (kDa)

6.84

Isoelectric Point (pI)

51.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 161 2.4e-20 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 144
AciI CCGC 2 cut(s) 218, 359
AcoI YGGCCR 2 cut(s) 115, 158
AfaI GTAC 2 cut(s) 71, 201
AfiI CCNNNNNNNGG 1 cut(s) 241
AgsI TTSAA 2 cut(s) 389, 430
AjnI CCWGG 1 cut(s) 93
AloI GAACNNNNNNTCC 2 cut(s) 345, 377
AluBI AGCT 2 cut(s) 307, 426
AluI AGCT 2 cut(s) 307, 426
Alw26I GTCTC 3 cut(s) 40, 125, 382
AoxI GGCC 2 cut(s) 115, 158
ApeKI GCWGC 1 cut(s) 423
AsuHPI GGTGA 2 cut(s) 176, 256
BbvI GCAGC 1 cut(s) 435
BccI CCATC 2 cut(s) 47, 431
BceAI ACGGC 1 cut(s) 150
BciT130I CCWGG 1 cut(s) 95
BcoDI GTCTC 3 cut(s) 40, 125, 382
BfaI CTAG 2 cut(s) 420, 490
BfmI CTRYAG 1 cut(s) 261
BisI GCNGC 1 cut(s) 424
BlsI GCNGC 1 cut(s) 425
Bme1390I CCNGG 1 cut(s) 95
BmiI GGNNCC 2 cut(s) 138, 258
BmrFI CCNGG 1 cut(s) 95
BmsI GCATC 1 cut(s) 160
BpmI CTGGAG 1 cut(s) 123
BsaI GGTCTC 2 cut(s) 40, 382
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse1I ACTGG 1 cut(s) 140
BseBI CCWGG 1 cut(s) 95
BseLI CCNNNNNNNGG 1 cut(s) 241
BseMII CTCAG 1 cut(s) 120
BseNI ACTGG 1 cut(s) 140
BseRI GAGGAG 1 cut(s) 366
BseXI GCAGC 1 cut(s) 435
BshFI GGCC 2 cut(s) 117, 160
BsiSI CCGG 2 cut(s) 114, 157
BslI CCNNNNNNNGG 1 cut(s) 241
BsmAI GTCTC 3 cut(s) 40, 125, 382
BsmBI CGTCTC 1 cut(s) 125
BsnI GGCC 2 cut(s) 117, 160
Bso31I GGTCTC 2 cut(s) 40, 382
BspACI CCGC 2 cut(s) 218, 359
BspANI GGCC 2 cut(s) 117, 160
BspCNI CTCAG 1 cut(s) 121
BspLI GGNNCC 2 cut(s) 138, 258
BspMAI CTGCAG 1 cut(s) 265
BspTNI GGTCTC 2 cut(s) 40, 382
BsrI ACTGG 1 cut(s) 140
Bst2UI CCWGG 1 cut(s) 95
Bst4CI ACNGT 1 cut(s) 446
Bst6I CTCTTC 1 cut(s) 404
BstAPI GCANNNNNTGC 1 cut(s) 328
BstC8I GCNNGC 1 cut(s) 261
BstDEI CTNAG 2 cut(s) 64, 129
BstEII GGTNACC 1 cut(s) 182
BstMAI GTCTC 3 cut(s) 40, 125, 382
BstMWI GCNNNNNNNGC 2 cut(s) 157, 328
BstNI CCWGG 1 cut(s) 95
BstPI GGTNACC 1 cut(s) 182
BstSCI CCNGG 1 cut(s) 93
BstSFI CTRYAG 1 cut(s) 261
BstV1I GCAGC 1 cut(s) 435
BsuRI GGCC 2 cut(s) 117, 160
Cac8I GCNNGC 1 cut(s) 261
CsiI ACCWGGT 1 cut(s) 93
Csp6I GTAC 2 cut(s) 70, 200
CviAII CATG 2 cut(s) 82, 119
CviJI RGCY 9 cut(s) 117, 137, 160, 230, 259, 269, 307, 385, 426
CviKI_1 RGCY 9 cut(s) 117, 137, 160, 230, 259, 269, 307, 385, 426
CviQI GTAC 2 cut(s) 70, 200
DdeI CTNAG 2 cut(s) 64, 129
EaeI YGGCCR 2 cut(s) 115, 158
Eam1104I CTCTTC 1 cut(s) 404
EarI CTCTTC 1 cut(s) 404
Eco31I GGTCTC 2 cut(s) 40, 382
Eco91I GGTNACC 1 cut(s) 182
EcoO65I GGTNACC 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 93
Esp3I CGTCTC 1 cut(s) 125
FaeI CATG 2 cut(s) 85, 122
FaiI YATR 6 cut(s) 20, 32, 44, 83, 120, 476
FatI CATG 2 cut(s) 81, 118
FblI GTMKAC 1 cut(s) 144
Fnu4HI GCNGC 1 cut(s) 424
Fsp4HI GCNGC 1 cut(s) 424
FspBI CTAG 2 cut(s) 420, 490
GluI GCNGC 1 cut(s) 424
GsuI CTGGAG 1 cut(s) 123
HaeIII GGCC 2 cut(s) 117, 160
HapII CCGG 2 cut(s) 114, 157
Hin1II CATG 2 cut(s) 85, 122
HinfI GANTC 1 cut(s) 164
HpaII CCGG 2 cut(s) 114, 157
HphI GGTGA 2 cut(s) 176, 256
Hpy166II GTNNAC 2 cut(s) 91, 145
Hpy188I TCNGA 3 cut(s) 106, 130, 256
Hpy188III TCNNGA 1 cut(s) 365
Hpy8I GTNNAC 2 cut(s) 91, 145
HpyAV CCTTC 2 cut(s) 235, 443
HpyCH4III ACNGT 1 cut(s) 446
HpyCH4V TGCA 4 cut(s) 22, 122, 263, 331
HpyF10VI GCNNNNNNNGC 2 cut(s) 157, 328
HpyF3I CTNAG 2 cut(s) 64, 129
Hsp92II CATG 2 cut(s) 85, 122
LmnI GCTCC 2 cut(s) 142, 256
Lsp1109I GCAGC 1 cut(s) 435
LweI GCATC 1 cut(s) 160
MabI ACCWGGT 1 cut(s) 93
MaeI CTAG 2 cut(s) 420, 490
MaeIII GTNAC 2 cut(s) 182, 440
MboII GAAGA 2 cut(s) 250, 421
MluCI AATT 1 cut(s) 7
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 5 cut(s) 197, 213, 219, 240, 344
MseI TTAA 1 cut(s) 303
MspI CCGG 2 cut(s) 114, 157
MspR9I CCNGG 1 cut(s) 95
MvaI CCWGG 1 cut(s) 95
MwoI GCNNNNNNNGC 2 cut(s) 157, 328
NlaIII CATG 2 cut(s) 85, 122
NlaIV GGNNCC 2 cut(s) 138, 258
NmeAIII GCCGAG 1 cut(s) 361
NmuCI GTSAC 1 cut(s) 182
PkrI GCNGC 1 cut(s) 425
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
Psp6I CCWGG 1 cut(s) 93
PspEI GGTNACC 1 cut(s) 182
PspGI CCWGG 1 cut(s) 93
PspN4I GGNNCC 2 cut(s) 138, 258
PstI CTGCAG 1 cut(s) 265
RsaI GTAC 2 cut(s) 71, 201
RsaNI GTAC 2 cut(s) 70, 200
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 1 cut(s) 424
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 95
SexAI ACCWGGT 1 cut(s) 93
SfaNI GCATC 1 cut(s) 160
SfcI CTRYAG 1 cut(s) 261
Sse9I AATT 1 cut(s) 7
SsiI CCGC 2 cut(s) 218, 359
SspMI CTAG 2 cut(s) 420, 490
StyD4I CCNGG 1 cut(s) 93
TaaI ACNGT 1 cut(s) 446
TasI AATT 1 cut(s) 7
TatI WGTACW 1 cut(s) 69
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TseFI GTSAC 1 cut(s) 182
TseI GCWGC 1 cut(s) 423
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 2 cut(s) 98, 384
XmiI GTMKAC 1 cut(s) 144
XspI CTAG 2 cut(s) 420, 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.