RLG00000023981

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004049
Physical Location & Seq
Forward (+)
30688569 .. 30689327
759 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023981

Sequence Viewer

Length: 483 bp
ATGGGAAATTGCTTGGTTCTGCAACAAAATGTTACAAAGATAATGAAACCAGATGGGAAAGTCCTAGAGTACAGAGCATCCATAAAAGTTTACCAGGTGTTGTCCGAGTTTTCTGGCCACGCAATATCTGAGACGACTCCGGTGTACCAGCATCTCCGGTCAGATGCAAAGCTGCTTGGTGGTCGCCTGTACTATCTTGTACCTCTTCCTTTGCCGTCAGAGAAGGCATCTCAGGAGAAGGTGAGGTTTTCAACTGATCCTGAAGGTGAAGCTCAACAAGAAACTAAAGTTGTAAGAATTAAGCTAGTTATAAGCAAGCAACAACTGCAGGAGATGCTGGGAAAGGGAGGGGTTTCAGTTGATGACATGGTTTCCAGAATTCAGCGTCAAACAAGCACAGATAAGAAGACTGACAGCGTCGACAACGATGGTAGTTGTGATGGGTGGAAACCAGTTTTAGAAAGCATACCTGAAGTAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

161

Amino Acids

17.82

Weight (kDa)

6.74

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 158 2.1e-22 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 311
AccI GTMKAC 1 cut(s) 420
AclWI GGATC 1 cut(s) 251
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 1 cut(s) 378
AcuI CTGAAG 1 cut(s) 282
AfaI GTAC 4 cut(s) 71, 146, 191, 201
AgsI TTSAA 1 cut(s) 252
AjnI CCWGG 1 cut(s) 93
AluBI AGCT 3 cut(s) 172, 272, 304
AluI AGCT 3 cut(s) 172, 272, 304
Alw26I GTCTC 1 cut(s) 125
AlwI GGATC 1 cut(s) 251
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 1 cut(s) 172
ApoI RAATTY 1 cut(s) 378
AsuHPI GGTGA 2 cut(s) 253, 278
BalI TGGCCA 1 cut(s) 117
BbsI GAAGAC 1 cut(s) 413
BbvI GCAGC 1 cut(s) 159
BccI CCATC 3 cut(s) 47, 422, 434
BceAI ACGGC 1 cut(s) 199
BciT130I CCWGG 1 cut(s) 95
BcoDI GTCTC 1 cut(s) 125
BfaI CTAG 3 cut(s) 65, 305, 481
BfmI CTRYAG 1 cut(s) 326
BisI GCNGC 1 cut(s) 173
BlsI GCNGC 1 cut(s) 174
Bme1390I CCNGG 1 cut(s) 95
BmrFI CCNGG 1 cut(s) 95
BmsI GCATC 5 cut(s) 86, 154, 160, 236, 324
BpiI GAAGAC 1 cut(s) 413
BsaWI WCCGGW 2 cut(s) 139, 156
Bse1I ACTGG 1 cut(s) 452
BseBI CCWGG 1 cut(s) 95
BseGI GGATG 1 cut(s) 77
BseMII CTCAG 2 cut(s) 120, 245
BseNI ACTGG 1 cut(s) 452
BseXI GCAGC 1 cut(s) 159
BseYI CCCAGC 1 cut(s) 337
BshFI GGCC 1 cut(s) 117
BsiSI CCGG 2 cut(s) 140, 157
BsmAI GTCTC 1 cut(s) 125
BsmBI CGTCTC 1 cut(s) 125
BsnI GGCC 1 cut(s) 117
Bsp143I GATC 1 cut(s) 256
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 2 cut(s) 121, 244
BspMAI CTGCAG 1 cut(s) 330
BspPI GGATC 1 cut(s) 251
BsrI ACTGG 1 cut(s) 452
BssMI GATC 1 cut(s) 256
Bst2UI CCWGG 1 cut(s) 95
Bst6I CTCTTC 1 cut(s) 210
BstAPI GCANNNNNTGC 2 cut(s) 325, 334
BstC8I GCNNGC 1 cut(s) 317
BstDEI CTNAG 2 cut(s) 129, 231
BstF5I GGATG 1 cut(s) 77
BstKTI GATC 1 cut(s) 259
BstMAI GTCTC 1 cut(s) 125
BstMBI GATC 1 cut(s) 256
BstMWI GCNNNNNNNGC 2 cut(s) 325, 334
BstNI CCWGG 1 cut(s) 95
BstSCI CCNGG 1 cut(s) 93
BstSFI CTRYAG 1 cut(s) 326
BstV1I GCAGC 1 cut(s) 159
BstV2I GAAGAC 1 cut(s) 413
BsuRI GGCC 1 cut(s) 117
BtsCI GGATG 1 cut(s) 77
Cac8I GCNNGC 1 cut(s) 317
CseI GACGC 2 cut(s) 374, 406
CsiI ACCWGGT 1 cut(s) 93
Csp6I GTAC 4 cut(s) 70, 145, 190, 200
CviAII CATG 1 cut(s) 367
CviJI RGCY 4 cut(s) 117, 172, 272, 304
CviKI_1 RGCY 4 cut(s) 117, 172, 272, 304
CviQI GTAC 4 cut(s) 70, 145, 190, 200
DdeI CTNAG 2 cut(s) 129, 231
DpnI GATC 1 cut(s) 258
DpnII GATC 1 cut(s) 256
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Eco57I CTGAAG 1 cut(s) 282
EcoRI GAATTC 1 cut(s) 378
EcoRII CCWGG 1 cut(s) 93
Esp3I CGTCTC 1 cut(s) 125
FaeI CATG 1 cut(s) 370
FaiI YATR 4 cut(s) 83, 311, 368, 467
FatI CATG 1 cut(s) 366
FblI GTMKAC 1 cut(s) 420
Fnu4HI GCNGC 1 cut(s) 173
FokI GGATG 1 cut(s) 64
Fsp4HI GCNGC 1 cut(s) 173
FspBI CTAG 3 cut(s) 65, 305, 481
GluI GCNGC 1 cut(s) 173
GsaI CCCAGC 1 cut(s) 341
HaeIII GGCC 1 cut(s) 117
HapII CCGG 2 cut(s) 140, 157
HgaI GACGC 2 cut(s) 374, 406
Hin1II CATG 1 cut(s) 370
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HinfI GANTC 1 cut(s) 136
HpaII CCGG 2 cut(s) 140, 157
HphI GGTGA 2 cut(s) 253, 278
Hpy166II GTNNAC 4 cut(s) 91, 145, 421, 478
Hpy188I TCNGA 4 cut(s) 106, 130, 163, 220
Hpy188III TCNNGA 3 cut(s) 233, 260, 375
Hpy8I GTNNAC 4 cut(s) 91, 145, 421, 478
Hpy99I CGWCG 1 cut(s) 422
HpyAV CCTTC 3 cut(s) 217, 232, 257
HpyCH4V TGCA 3 cut(s) 22, 167, 328
HpyF10VI GCNNNNNNNGC 2 cut(s) 325, 334
HpyF3I CTNAG 2 cut(s) 129, 231
Hsp92II CATG 1 cut(s) 370
Kzo9I GATC 1 cut(s) 256
Lsp1109I GCAGC 1 cut(s) 159
LweI GCATC 5 cut(s) 86, 154, 160, 236, 324
MabI ACCWGGT 1 cut(s) 93
MaeI CTAG 3 cut(s) 65, 305, 481
MaeIII GTNAC 1 cut(s) 31
MalI GATC 1 cut(s) 258
MboI GATC 1 cut(s) 256
MboII GAAGA 2 cut(s) 197, 418
MlsI TGGCCA 1 cut(s) 117
MluCI AATT 3 cut(s) 7, 297, 378
MluNI TGGCCA 1 cut(s) 117
MlyI GAGTC 1 cut(s) 130
MnlI CCTC 3 cut(s) 213, 237, 341
Mox20I TGGCCA 1 cut(s) 117
MscI TGGCCA 1 cut(s) 117
MseI TTAA 1 cut(s) 300
Msp20I TGGCCA 1 cut(s) 117
MspI CCGG 2 cut(s) 140, 157
MspR9I CCNGG 1 cut(s) 95
MvaI CCWGG 1 cut(s) 95
MwoI GCNNNNNNNGC 2 cut(s) 325, 334
NdeII GATC 1 cut(s) 256
NlaIII CATG 1 cut(s) 370
PflFI GACNNNGTC 1 cut(s) 416
PkrI GCNGC 1 cut(s) 174
PleI GAGTC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 130
PsiI TTATAA 1 cut(s) 311
Psp6I CCWGG 1 cut(s) 93
PspFI CCCAGC 1 cut(s) 337
PspGI CCWGG 1 cut(s) 93
PstI CTGCAG 1 cut(s) 330
PsyI GACNNNGTC 1 cut(s) 416
RsaI GTAC 4 cut(s) 71, 146, 191, 201
RsaNI GTAC 4 cut(s) 70, 145, 190, 200
SalI GTCGAC 1 cut(s) 419
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 1 cut(s) 173
Sau3AI GATC 1 cut(s) 256
SchI GAGTC 1 cut(s) 130
ScrFI CCNGG 1 cut(s) 95
SetI ASST 9 cut(s) 99, 174, 205, 243, 248, 268, 274, 306, 472
SexAI ACCWGGT 1 cut(s) 93
SfaNI GCATC 5 cut(s) 86, 154, 160, 236, 324
SfcI CTRYAG 1 cut(s) 326
Sse9I AATT 3 cut(s) 7, 297, 378
SspMI CTAG 3 cut(s) 65, 305, 481
StyD4I CCNGG 1 cut(s) 93
TaqI TCGA 1 cut(s) 420
TasI AATT 3 cut(s) 7, 297, 378
TatI WGTACW 2 cut(s) 69, 189
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TseI GCWGC 1 cut(s) 172
TspDTI ATGAA 1 cut(s) 59
Tth111I GACNNNGTC 1 cut(s) 416
XapI RAATTY 1 cut(s) 378
XmiI GTMKAC 1 cut(s) 420
XspI CTAG 3 cut(s) 65, 305, 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.